CN107114414A - The application of the anti-Wolfberry Aphid of The Leaves And Stems of Stellera Chamaejasme extract - Google Patents
The application of the anti-Wolfberry Aphid of The Leaves And Stems of Stellera Chamaejasme extract Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
Abstract
Wolfberry Aphid is the Severe pests of matrimony vine woods, causes yield of medlar decline, blade and nutrient constituents of fruit and micronutrient levels to change.Long-term a large amount of use of chemical pesticide brings a variety of drawbacks, and Chinese Stellera Root can the utilization to Chinese Stellera Root has certain defect as biogenic pesticide, but at present, and whole strain, which is utilized, to be caused to gather whole strain Chinese Stellera Root, it is necessary to which constantly plantation, increases cost.The invention provides a kind of The Leaves And Stems of Stellera Chamaejasme extract for anti-Wolfberry Aphid, the extract contains Chinese Stellera Root flavones, is obtained by processes such as alcohol steep, chromatography, ethyl acetate and/or n-butanol elutions.The present invention replaces root as raw material using aerial part, both avoids the destruction that excavation root causes to be difficult to recover to vegetation, topsoil, the harm that the root of langdu wreaks havoc grassland can be efficiently controlled again.
Description
Technical field
The invention belongs to plant protection art, and in particular to the application of the anti-Wolfberry Aphid of The Leaves And Stems of Stellera Chamaejasme extract.
Background technology
Wolfberry Aphid（Aphid gosypiiGlover）It is the Severe pests of matrimony vine woods, it is difficult to control, is often grown in matrimony vine
Season population outbreak, causes fructus lycii yield reduction 30%~70%, product quality significantly declines, and commodity value is by serious shadow
Ring.The harm of Wolfberry Aphid mainly causes yield of medlar decline, blade and nutrient constituents of fruit and micronutrient levels to become
Change.When insect density reaches 6.3/haw, that is, there is production loss.Insect density is higher, and production loss is bigger, insect population
It is in logarithm clear-cut correlation between density and yield.Preventing and treating Wolfberry Aphid and the yield and quality of matrimony vine is improved has important meaning
Justice.
Chemical pesticide it is long-term a large amount of using bringing a variety of drawbacks, the generation of such as harmful biomolecule resistant, residual hazard and
Environmental pollution, kill a large amount of pest natural enemies in which cannot tell the good from the bad, the heavy damage ecological balance.Therefore, meet environmental protection, health, hold
Continuous the efficient of idea of development, low toxicity, low-residual, the biological pesticide exploitation that is compatible with the environment just turn into the master of current pesticide research
Topic.Biological pesticide because its safety non-toxic, noresidue, it is free from environmental pollution be public well-established and develop rapidly.90 years 20th century
Since generation, whole world chemical pesticide sales volume is constantly declined with 1%~2% speed, and biological pesticide is with 20% speed
Be incremented by, absolutely prove biological pesticide in environment, the great potential in social benefit.
Chinese Stellera Root (Stellera Chamaejasmel L.) is under the jurisdiction of Thymelaeceae stellera herbaceos perennial,
Also known as gelsemium elegan, toxic, its root is relieved oedema or abdominal distension through diuresis or purgation eliminating the phlegm, the effect of dissipating bind desinsection.Stem coca is thick, and matter is tough to be not easily broken, and bark has
Silk floss fiber；Aerial part is in filbert, and branch, smooth not hairless；Leaf lanceolar, basidixed capitulum, flower yellow or white,
Fruit is that pyrene is avette, and seed yield is big, and Seed Vitality is strong, 6~August of florescence, 9~October of fruiting period.Chinese Stellera Root is more
The endroit that height above sea level is higher, year-round average temperature is at 0 DEG C or so is grown in, China northeast, northwest, southwest etc. is distributed widely in
Ground.Chinese Stellera Root root drought resistance and competitiveness are extremely strong, and the obvious strongly fragrant effect of covering can be produced to other plant kind around.In addition,
Chinese Stellera Root also has certain Ecology Action in addition to some of medical value, is also used as biogenic pesticide, such as desinsection
Agent.
The insecticidal activity of Chinese Stellera Root is mainly reflected in it as the application in terms of botanical pesticide.Cao is waved etc. to winter daphne wolf
Poison root extract carries out determination of activity and finds that the insecticidal effect of Chinese Stellera Root shows as tagging and stomach poison activity.Li Jie etc. is with a variety of
Assay method compares the insecticidal effect of 4 kinds of different extracts of chameajasme, it is found that it is respectively provided with good insecticidal effect.High equality
Desk research and field test are carried out to the toxicity of tagging of cotten aphid, citrus aphid and cigarette aphid, antifeedant activity by SCEE, as a result table
It is bright：Chameajasme extract is fine to the insecticidal action of a few class aphids, but due to the difference in aphid source, insecticidal effect exists certain
Difference.Hou Taiping obtains 2 kinds of potent aphid components of going out from the root of langdu：1,5- diphenyl-1- pentanones and 1,5-2-1-diphenyl
Pi-allyl cyclopentanone, and using it as the main component of aphid biological removal agent, and field experiment effect is fine.But at present to auspicious
There is certain defect in the utilization of the fragrant root of langdu, whole strain, which is utilized, to be caused to gather whole strain Chinese Stellera Root, it is necessary to which constantly plantation, makes cost
Increase.
The content of the invention
To solve the deficiency in prior art, the invention provides answering for the anti-Wolfberry Aphid of The Leaves And Stems of Stellera Chamaejasme extract
With.
It is an object of the invention to provide the application of the anti-Wolfberry Aphid of The Leaves And Stems of Stellera Chamaejasme extract, substituted using biological pesticide
Chemical pesticide, utilizes cauline leaf, it is to avoid excavation root is to vegetation, the destruction of soil.
Technical scheme is as follows：
The present invention provides a kind of The Leaves And Stems of Stellera Chamaejasme extract for anti-Wolfberry Aphid, and it is yellow that the extract contains Chinese Stellera Root
Ketone, and obtained according to following process：
Cauline leaf dry powder 1kg, solid-to-liquid ratio 1 are weighed respectively：25-30, plus ethanol immersion, ultrasonic 50min, are extracted 2 times, filtering, filter residue
Repeat to extract, concentrated by rotary evaporation obtains just medicinal extract.After first medicinal extract and silica gel mixed sample, revolving, load in chromatographic column, according to polarity size
Eluted successively with organic solvent, obtained two kinds of different separation components.
It is preferred that, tomographic system solvent is ethyl acetate phase or n-butanol phase.
It is preferred that, the concentration of the extract is 0.1-0.0005mg/ml.
It is preferred that, the concentration of the extract is 0.01,0.005,0.0025mg/ml.
Described extract can be made as missible oil, aqueous suspension agent, oil-suspending agent, wettable powder, granule or moisture
Acceptable formulation on the agricultural chemicals such as shot agent.
Described extract formulation be included in it is acceptable on agricultural chemicals, one or more be selected from wetting agent, dispersant, thickening
Agent, antifreezing agent, disintegrant, binding agent, defoamer, the auxiliary agent of carrier or filler.
A kind of preparation method of The Leaves And Stems of Stellera Chamaejasme extract for anti-Wolfberry Aphid, the preparation method includes following
Step：
（1）The extraction of Chinese Stellera Root active insecticidal components
The Chinese Stellera Root powder 2kg of drying and crushing is weighed, according to solid-liquid ratio 1：25-30, add 95% ethanol, cold soaking is after 3 days,
Ultrasonic wave extraction 2 times, each 60min.Extraction three times is repeated, filters and merges 3 filtrates, depressurized using Rotary Evaporators
Concentration, when being concentrated into 1/10 amount, pours out placement, carries out precipitation desugar, and sugar is separated out with crystalline state.Liquid rotated evaporimeter again
It is concentrated to give just medicinal extract.
（2）The separation of Chinese Stellera Root active insecticidal components
According to flow chart 1 is extracted, Chinese Stellera Root ethanol phase medicinal extract dissolves the silica gel for admixing 10 times of amounts with ethanol, is steamed in rotating thin film
After hair instrument is evaporated, loads in chromatographic column and carry out elution separation with ethyl acetate, n-butanol successively, pass through thin plate detection and elution amount
According to replacing eluting solvent is carried out, to respectively obtain different separated parts, i.e. ethyl acetate phase and n-butanol phase.Will be variant
It is standby after solvent is mutually dried.
It is preferred that, the step（1）Middle solid-liquid ratio 1：25 or 1：30.
Application of the extract in preventing and treating Wolfberry Aphid.
It is preferred that, application of the extract in preventing and treating matrimony vine 3 age aphid
The beneficial effects of the invention are as follows：Root is replaced as raw material using aerial part, excavation root is both avoided to vegetation, top layer
Soil causes the destruction for being difficult to recover, and the harm that the root of langdu wreaks havoc grassland can be efficiently controlled again.Meanwhile, solve change evil into
Treasured, is conducive to preventing and kill off and developing for the root of langdu.
Brief description of the drawings
Fig. 1 is Chinese Stellera Root extraction process flow chart.
Fig. 2 is the insect resistant effect of cauline leaf crude extract.
Fig. 3 is anti-insect activity research of the crude extract of cauline leaf and root to aphid.
The anti-insect activity of the ethyl acetate phase of Fig. 4 cauline leafs Wolfberry Aphid relative with cauline leaf n-butanol compares.
Anti-insect activity research of Fig. 5 cauline leafs difference separation phase component to aphid.
Embodiment
Further detailed description is done to the present invention with reference to embodiment：
The The Leaves And Stems of Stellera Chamaejasme of embodiment 1, root extract are extracted
1.1 experiment material
Material：Chameajasme, cauline leaf, cabbage caterpillar, beet exigua larvae.
Reagent：Rutin standard items, ethanol, n-butanol, ethyl acetate, natrium nitrosum, aluminum nitrate, sodium hydroxide etc. and other
AR and distilled water.
The extraction of 1.2 Chinese Stellera Root active insecticidal components
The Chinese Stellera Root powder 2kg of drying and crushing is weighed, according to solid-liquid ratio 1：30, the ethanol of addition 95%, cold soaking is after 3 days, ultrasound
Ripple is extracted 2 times, each 60min.Extraction three times is repeated, filters and merges 3 filtrates, it is dense to carry out decompression using Rotary Evaporators
Contracting, when being concentrated into 1/10 amount, pours out placement, carries out precipitation desugar, and sugar is separated out with crystalline state.Rotated evaporimeter is dense again for liquid
Contract to obtain first medicinal extract.
The separation of 1.3 Chinese Stellera Root active insecticidal components
According to Fig. 1, Chinese Stellera Root ethanol phase medicinal extract dissolves the silica gel for admixing 10 times of amounts with ethanol, is evaporated in rotating thin film evaporimeter
Afterwards, load in chromatographic column and carry out elution separation with ethyl acetate, n-butanol successively, be that foundation is entered by thin plate detection and elution amount
Row changes eluting solvent, respectively obtains different separated parts, i.e. ethyl acetate phase and n-butanol phase.Variant solvent is mutually dried
It is standby after dry.
The The Leaves And Stems of Stellera Chamaejasme of embodiment 2 and root extract anti-insect activity compare
2.1 Chinese Stellera Root biological activity determination methods
2.1.1 the anti-insect activity assay method of The Leaves And Stems of Stellera Chamaejasme and root crude extract
Take each 0.1g of crude extract of The Leaves And Stems of Stellera Chamaejasme and root, with 10mL acetone solutions as sample, control group 10mL acetone
Make same processing.
2.1.1.1 its Antifeedant Effect Against method
The measure of antifeedant activity is carried out using dipping leaf dish feeding method.Fresh cabbage leaf is cut into the cm of 1 cm × 1 leaf
Dish, is put into 2 ~ 3s of leaching in the acetone dilution of test sample, dries naturally.A metafiltration is spread in a diameter of 9 cm culture dish bottom
Paper, and add distilled water moisturizing, the leaf dish that correspondence medicine processing is put into each ware is a piece of, and the leaf dish crossed with acetone treatment is compared.
Picking is in the same size, hungry 4h 3 age cabbage caterpillar, beet exigua larvae, 3 instar larvae 1 is put into each culture dish, with 20
For one group, if 3 repetitions.Raise at room temperature, add the treated leaf dish one of same procedure after 1 leaf dish again if being eaten up for test worm
Piece, record takes food the quantity of leaf dish.Leaf area is taken food every 24h graph papers record, and changes the malicious blade of fresh leaching, continuous note
72h is recorded, anti-food rate is calculated according to the following formula.
Anti-food rate=（Control group averagely takes food leaf area-treatment group and averagely takes food leaf area）/ control group averagely takes food leaf
Area × 100%
2.1.1.2 Contact toxicity assay method
The measure of Contact toxicity is carried out using dip method.The cabbage leaf of cleaning is put into each culture dish.From the big of hungry 4h
In small 3 consistent age cabbage caterpillars, beet exigua larvae larva, acetone dilution and comparison liquid that larva is entered to test sample, leaching 2
Taken out after ~ 3s, treat that decoction dries naturally, larva is put in culture dish.Every group of 20 cephalonts, are repeated 3 times.Respectively at after processing
24h, 48h and 72h are observed, and the death rate is calculated according to following equation note.
Corrected mortality=（The treatment group death rate-control group the death rate）/ (1-control group death rate) × 100%
2.1.2 The Leaves And Stems of Stellera Chamaejasme and root extract difference separate the anti-insect activity assay method of phase
The ethyl acetate phase of Example 1, mutually each 0.1g of n-butanol, sample, control group 10mL third are used as with 10mL acetone solutions
Ketone makees same processing.
2.1.2.1 its Antifeedant Effect Against method
Experimental method is with " 2.1.1.1 " its Antifeedant Effect Against method.
2.1.2.2 Contact toxicity assay method
Experimental method is with " 2.1.1.2 " Contact toxicity assay method.
2.2 experimental results and analysis
2.2.1 the anti-insect activity of The Leaves And Stems of Stellera Chamaejasme and root crude extract
2.2.1.1 The Leaves And Stems of Stellera Chamaejasme and root crude extract are to cabbage caterpillar, the Antifeedant Effects of beet exigua larvae
As can be seen from Table 1 during 24h, the crude extract of cauline leaf and root reaches highest, root crude extract to the average anti-food rate of cabbage caterpillar
Average anti-food rate to cabbage caterpillar is 82.61%, and cauline leaf crude extract is 84.06% to the average anti-food rate of cabbage caterpillar；4 treatment groups
Take food area notable with contrast difference.
As can be seen from Table 2 during 24h, the crude extract of cauline leaf and root reaches most to the average anti-food rate of beet exigua larvae
Height, root crude extract is 81.69% to the average anti-food rate of beet exigua larvae, and cauline leaf crude extract is averaged to beet exigua larvae
Anti-food rate is 82.39%；It is notable with contrast difference that 4 treatment groups take food area.
It can be drawn by Tables 1 and 2, the crude extract of cauline leaf and root to cabbage caterpillar, beet exigua larvae to having well
Antifeedant Effects, cauline leaf crude extract is better than root crude extract.
The The Leaves And Stems of Stellera Chamaejasme crude extract of table 1 and Antifeedant Effects of the root crude extract to cabbage caterpillar
The The Leaves And Stems of Stellera Chamaejasme crude extract of table 2 and Antifeedant Effects of the root crude extract to beet exigua larvae
2.2.1.2 The Leaves And Stems of Stellera Chamaejasme and root crude extract are to cabbage caterpillar, the action of contace poison of beet exigua larvae
As can be seen from Table 3, cauline leaf and root crude extract are to cabbage caterpillar, the action of contace poison extension over time of beet exigua larvae
Bioactivity strengthens.After 72h, cauline leaf and root crude extract all reach highest to the corrected mortality of cabbage caterpillar and beet armyworm children,
Root crude extract is respectively 100% and 99.86% to the corrected mortality of cabbage caterpillar and beet armyworm children, and cauline leaf crude extract is respectively
99.96% and 100%.Cauline leaf crude extract is to cabbage caterpillar, the corrected mortality when height of root crude extract always of beet armyworm children.
In summary, the crude extract of cauline leaf and root is overall to there is fine pest-resistant effect to cabbage caterpillar, beet exigua larvae
For, cauline leaf crude extract is better than root crude extract.
The The Leaves And Stems of Stellera Chamaejasme crude extract of table 3 is with root crude extract to cabbage caterpillar, the action of contace poison of beet exigua larvae
2.2.2 The Leaves And Stems of Stellera Chamaejasme and root crude extract difference separate the anti-insect activity of phase
2.2.2.1 The Leaves And Stems of Stellera Chamaejasme and root crude extract difference separate relative cabbage caterpillar, the Antifeedant Effects of beet exigua larvae
As can be seen from Table 4, after 24h, the anti-food rate of each relative cabbage caterpillar reaches highest.Root ethyl acetate phase anti-food rate reaches
90.85%, cauline leaf ethyl acetate phase anti-food rate reaches 92.25%, and root n-butanol phase anti-food rate reaches 65.49%, cauline leaf n-butanol phase
Anti-food rate reaches 69.72%.It is notable with contrast difference that 4 treatment groups take food area.
As can be seen from Table 5, after 24h, the anti-food rate of each relative beet exigua larvae reaches highest.Root ethyl acetate phase is refused
Food rate reaches 90.41%, and cauline leaf ethyl acetate phase anti-food rate reaches 92.47%, and root n-butanol phase anti-food rate reaches 65.07% cauline leaf
N-butanol phase anti-food rate reaches 68.49%.It is notable with contrast difference that 4 treatment groups take food area.
The Antifeedant Effects of the The Leaves And Stems of Stellera Chamaejasme of table 4 and root crude extract different solvents with respect to cabbage caterpillar
The Antifeedant Effects of the The Leaves And Stems of Stellera Chamaejasme of table 5 and root crude extract different solvents with respect to beet exigua larvae
In same concentrations same time it can be seen from table 4 and table 5, the ethyl acetate phase of The Leaves And Stems of Stellera Chamaejasme and root crude extract
Biological activity ratio's n-butanol it is mutually high.
2.2.2.2 The Leaves And Stems of Stellera Chamaejasme and root crude extract difference separate relative cabbage caterpillar, the work of tagging of beet exigua larvae
With
As can be seen from Table 6, ethyl acetate phase corrected mortality is mutually higher than n-butanol, and bioactivity is strong.
After 24h, root ethyl acetate is respectively 71.86% He with respect to the corrected mortality of cabbage caterpillar and beet armyworm children
70.41%, it is respectively 73.58% and 71.96% followed by cauline leaf ethyl acetate phase；Root n-butanol is with respect to cabbage caterpillar and beet night
The corrected mortality of moth children is respectively 50.51% and 49.07%, and cauline leaf n-butanol phase is respectively 52.46%, 50.09%；After 48h,
Each phase bioactivity is further enhanced, and corrected mortality all increased；After 72h, root, cauline leaf ethyl acetate with respect to cabbage caterpillar and
The corrected mortality of beet armyworm children all reaches that absolutely, root, cauline leaf n-butanol has mutually also all reached more than 90 percent.
Proved by data above, in same concentrations same time, acetic acid ethyl ester extract is tagged to cabbage caterpillar and beet armyworm children
Activity is most strong, and n-butyl alcohol extract activity is weaker.
The The Leaves And Stems of Stellera Chamaejasme of table 6 and root crude extract different solvents are with respect to cabbage caterpillar, the action of contace poison of beet exigua larvae
It is above-mentioned test result indicates that, cauline leaf separates relative cabbage caterpillar, beet exigua larvae with root extract and its difference and is respectively provided with
Certain anti-insect activity.On the whole, the anti-insect activity of cauline leaf crude extract is better than root crude extract；Same concentrations same time
Interior, biological activity ratio's n-butanol of the ethyl acetate phase of The Leaves And Stems of Stellera Chamaejasme and root crude extract is mutually high.
The anti-Wolfberry Aphid effect of the The Leaves And Stems of Stellera Chamaejasme extract of embodiment 3
3.1. anti-insect activity assay method of the The Leaves And Stems of Stellera Chamaejasme extract to Wolfberry Aphid
3.1. anti-insect activity assay method of the 1 The Leaves And Stems of Stellera Chamaejasme crude extract to Wolfberry Aphid
The crude extract of the The Leaves And Stems of Stellera Chamaejasme extracted and different separation are mutually taken it is a certain amount of, it is dilute with certain density acetone
Liquid dilution is released, configuration sample solution concentration is respectively 0.01,0.005,0.0025mg/ml.With acetone as a control group.
（1）Contact toxicity is determined
According to Zhang Zong Ping method, the insect resistant effect that direct impregnation checks each component is carried out to tried aphid.Take a certain size
Plate, spreads a metafiltration paper and a fresh matrimony vine blade of the same size, and add 1ml distilled water with pipettor in bottom respectively
Keep humidity.The 3 age aphid 30 of indoor feeding is chosen, each concentration for the treatment of is 90.According to the order of concentration from low to high,
Immersion 10s during three age aphids are placed in gauze in each test sample and comparison liquid prepared in advance respectively, taking-up is drained, and is inhaled
Decoction is removed, is placed in ready plate, closes the lid to prevent aphid from climbing mistake.
Above-mentioned each treatment group is repeated 3 times, be placed in (25 ± 1) DEG C, relative humidity 80% or so, photoperiod be L/D=
In 16/8 h incubator.Every 12h, check dead with surviving number, calculate the death rate, continuously record 72h.According to following public affairs
Formula calculates corrected mortality.
Corrected mortality=（The experimental group aphid death rate-control group aphid death rate）/（The 1- control group aphid death rates）×
100%
（2）Interior suction toxicity test
Using the method that first leaching leaf is followed by worm.By without drug-treated and without aphid worm's ovum matrimony vine blade, take size one
Cause, in being soaked 15 seconds in the decoction configured, taking-up is dried, and is put into the plate containing filter paper and moistening handled well, each ware
The middle 3 age aphid 30 for moving into indoor feeding with writing brush respectively, each concentration for the treatment of is 90.Test in triplicate.
3.1.2 the anti-insect activity of the ethyl acetate phase of cauline leaf Wolfberry Aphid relative with cauline leaf n-butanol compares
The Leaves And Stems of Stellera Chamaejasme ethyl acetate phase and n-butanol are diluted with acetone, be each configured to concentration for 0.01,
0.005mg/ml sample solution, with acetone as a control group.Its Contact toxicity and interior drug abuse power are determined respectively, and method is same
“3.1.1”。
3.2. result and analysis
3.2.1 the anti-insect activity analysis of The Leaves And Stems of Stellera Chamaejasme crude extract
3.2. 1.1 contact toxicities are analyzed
Cauline leaf crude extract is studied by dip method to find the prevention effect of aphid, cauline leaf crude extract has aphid obvious preventing and treating
Effect, crude extract concentration is directly proportional to corrected mortality, and concentration is higher, and anti-insect activity is more obvious；Anti-insect activity has obvious
Time dependence（Fig. 2）.
3.2. 1.2 systemic activities are analyzed
When the anti-insect activity studies have shown that difference crude extract concentration of Wolfberry Aphid is carried out to cauline leaf crude extract using leaf dipping method, stem
The anti-insect activity significant difference of Leave extract, concentration is higher, and anti-insect activity is stronger；And its anti-insect activity have the regular hour according to
Lai Xing（Fig. 3）.
3.2.2 the anti-insect activity of The Leaves And Stems of Stellera Chamaejasme extract difference separation phase compares
3.2.2.1 contact toxicity is analyzed
Tested by above-mentioned two, choose cauline leaf ethyl acetate phase and n-butanol that concentration is respectively 0.01 and 0.005 mg/ml
It is compared as 2 dosage, as a result as shown in Figure 4：Cauline leaf ethyl acetate phase reaches 0.01 with n-butanol phase concentration
During mg/ml, the anti-insect activity of cauline leaf ethyl acetate phase is notable；It is 0.005 in cauline leaf ethyl acetate phase and n-butanol phase concentration
During mg/ml, the anti-insect activity of cauline leaf ethyl acetate phase is slightly better than cauline leaf n-butanol phase.As a result show, cauline leaf ethyl acetate phase with
N-butanol is in same concentrations, and the ethyl acetate phase insect resistant effect of stem-leaf extract is mutually more notable than n-butanol.
3.2.2.2 systemic activity
The anti-insect activity research of Wolfberry Aphid is mutually carried out with cauline leaf n-butanol to cauline leaf ethyl acetate phase using leaf dipping method, from stem
Leaves ethyl acetate phase and the concentration of n-butanol phase are respectively 0.01 to carry out anti-insect activities with 0.005 mg/ml, two dosage and compared,
As a result as shown in figure 5, when cauline leaf ethyl acetate phase with n-butanol in same concentrations when, cauline leaf ethyl acetate phase treatment group it is anti-
Worm effect is significantly higher than cauline leaf n-butanol phase, and anti-insect activity has regular hour dependence.
This experiment has selected The Leaves And Stems of Stellera Chamaejasme crude extract and its different separation mutually to carry out pest-resistant experiment respectively and carry out
Expression activitiy, research shows that stem-leaf extract and its relative Wolfberry Aphid of different separation are respectively provided with certain anti-insect activity, cauline leaf
Ethyl acetate phase effect is mutually better than n-butanol, all with certain concentration-time depended.
Described above is only the preferred embodiment of this patent, it is noted that for the ordinary skill people of the art
For member, on the premise of the art of this patent principle is not departed from, some improvement and replacement can also be made, these improve and replaced
Also it should be regarded as the protection domain of this patent.
Claims (10)
1. a kind of The Leaves And Stems of Stellera Chamaejasme extract for anti-Wolfberry Aphid, it is characterised in that：The extract contains winter daphne wolf
Malicious flavones, and obtained according to following process：
Cauline leaf dry powder 1kg, solid-to-liquid ratio 1 are weighed respectively：25-30, plus ethanol immersion, ultrasonic 50min, are extracted 2 times, filtering, filter residue
Repeat to extract, concentrated by rotary evaporation obtains just medicinal extract；After first medicinal extract and silica gel mixed sample, revolving, load in chromatographic column, according to polarity size
Eluted successively with organic solvent, obtained different separation components.
2. a kind of The Leaves And Stems of Stellera Chamaejasme extract for anti-Wolfberry Aphid according to claim 1, it is characterised in that：Layer
The described organic solvent of analysis elution is ethyl acetate phase and/or n-butanol phase.
3. a kind of The Leaves And Stems of Stellera Chamaejasme extract for anti-Wolfberry Aphid according to claim 2, it is characterised in that：Institute
The concentration for stating extract is 0.1-0.0005mg/ml.
4. a kind of The Leaves And Stems of Stellera Chamaejasme extract for anti-Wolfberry Aphid according to claim 3, it is characterised in that：Institute
The concentration for stating extract is 0.01,0.005,0.0025mg/ml.
5. a kind of preparation method of The Leaves And Stems of Stellera Chamaejasme extract for anti-Wolfberry Aphid according to claim 1-4,
It is characterized in that：Described extract can be made as missible oil, aqueous suspension agent, oil-suspending agent, wettable powder, granule or water
Acceptable formulation on the agricultural chemicals such as dispersible granule.
6. a kind of preparation method of The Leaves And Stems of Stellera Chamaejasme extract for anti-Wolfberry Aphid according to claim 1, its
It is characterised by：Described extract formulation be included in it is acceptable on agricultural chemicals, one or more be selected from wetting agent, dispersant, increasing
Thick dose, antifreezing agent, disintegrant, binding agent, defoamer, the auxiliary agent of carrier or filler.
7. a kind of preparation method of The Leaves And Stems of Stellera Chamaejasme extract for anti-Wolfberry Aphid, the preparation method includes following step
Suddenly：
（1）The extraction of Chinese Stellera Root active insecticidal components
The Chinese Stellera Root powder 2kg of drying and crushing is weighed, according to solid-liquid ratio 1：25-30, add 95% ethanol, cold soaking is after 3 days,
Ultrasonic wave extraction 2 times, each 60min；Extraction three times is repeated, filters and merges 3 filtrates, depressurized using Rotary Evaporators
Concentration, when being concentrated into 1/10 amount, pours out placement, carries out precipitation desugar, and sugar is separated out with crystalline state；Liquid rotated evaporimeter again
It is concentrated to give just medicinal extract；
（2）The separation of Chinese Stellera Root active insecticidal components
Chinese Stellera Root ethanol phase medicinal extract dissolves the silica gel for admixing 10 times of amounts with ethanol, after rotating thin film evaporimeter is evaporated, and loads
Elution separation is carried out with ethyl acetate, n-butanol successively in chromatographic column, is that foundation is changed by thin plate detection and elution amount
Eluting solvent, respectively obtains different separated parts, i.e. ethyl acetate phase and n-butanol phase；After variant solvent is mutually dried,
It is standby.
8. a kind of preparation method of The Leaves And Stems of Stellera Chamaejasme extract for anti-Wolfberry Aphid according to claim 7, its
It is characterised by：The step（1）Middle solid-liquid ratio 1：25 or 1：30.
9. a kind of The Leaves And Stems of Stellera Chamaejasme extract for anti-Wolfberry Aphid according to claim 1-4, it is characterised in that：
Application of the extract in preventing and treating Wolfberry Aphid.
10. a kind of The Leaves And Stems of Stellera Chamaejasme extract for anti-Wolfberry Aphid according to claim 1-4, its feature exists
In：Application of the extract in preventing and treating matrimony vine 3 age aphid.
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