CN104273127B - A kind of pesticides composition for killing nematodes containing lantadene first element and amino-oligosaccharide - Google Patents
A kind of pesticides composition for killing nematodes containing lantadene first element and amino-oligosaccharide Download PDFInfo
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- CN104273127B CN104273127B CN201410574694.XA CN201410574694A CN104273127B CN 104273127 B CN104273127 B CN 104273127B CN 201410574694 A CN201410574694 A CN 201410574694A CN 104273127 B CN104273127 B CN 104273127B
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- 239000000203 mixture Substances 0.000 title claims abstract description 18
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- 241000244206 Nematoda Species 0.000 title claims description 26
- 230000002147 killing effect Effects 0.000 title claims description 7
- 239000004480 active ingredient Substances 0.000 claims abstract description 6
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- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 6
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- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 238000011835 investigation Methods 0.000 description 6
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- 231100000572 poisoning Toxicity 0.000 description 5
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 241000243785 Meloidogyne javanica Species 0.000 description 4
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- 229920002101 Chitin Polymers 0.000 description 1
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- ABUGVBRDFWGJRD-CHOYNLESSA-N [9-[(2r,3r,4s,5r)-3,4-dihydroxy-5-methyloxolan-2-yl]-2-(2,4-dinitrophenyl)sulfanylpurin-6-yl] [hydroxy(phosphonooxy)phosphoryl] hydrogen phosphate Chemical compound O[C@@H]1[C@H](O)[C@@H](C)O[C@H]1N1C2=NC(SC=3C(=CC(=CC=3)[N+]([O-])=O)[N+]([O-])=O)=NC(OP(O)(=O)OP(O)(=O)OP(O)(O)=O)=C2N=C1 ABUGVBRDFWGJRD-CHOYNLESSA-N 0.000 description 1
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- DUFVKSUJRWYZQP-UHFFFAOYSA-N fosthiazate Chemical compound CCC(C)SP(=O)(OCC)N1CCSC1=O DUFVKSUJRWYZQP-UHFFFAOYSA-N 0.000 description 1
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Abstract
The invention provides a kind of composition pesticide that can effectively prevent and treat root of the crop Root-knot. The constituent of the active ingredient of described composition pesticide is lantadene first element and amino-oligosaccharide. This composition pesticide can be made into aqua, missible oil, pulvis, granule, tablet etc. for preventing and treating root of the crop Root-knot. Result of practical application shows, it is remarkable that the granule of being prepared by this composition pesticide has the effect of control root of the crop Root-knot, to crop safety, and can conditioned soil loam matter, promote the features such as crop root growth.
Description
Technical field
The invention relates to a kind of pesticides composition for killing nematodes containing lantadene first element and amino-oligosaccharide, belong to technical field of pesticide.
Background technology
Over nearly 10 years, along with the adjustment of agricultural planting structure, in China's development rapidly, the ground of the existing protection of China at present vegetables area has exceeded 3,000,000 squares of hectares to the cultivation of protection ground. The development on protection ground, also, for generation, the development of root-knot nematode provide adapt circumstance, plants after 3 ~ 5 years conventionally, and the yield and quality of crop is subject to serious impact, the general underproduction 20% ~ 40%, and Severe Reduction more than 60%, even has no harvest. Along with the continuation development on protection ground and the cultivation year after year of high added value crop, the problem of root-knot nematode will be more and more outstanding, and protect by becoming serious restriction the key factor developing. Nemas is the disease being caused by nematode. Plant nematode, with the naked eye cannot see, main harm crop root and underground stem tuber, lepisphere stem, also can endanger the parts such as stem, leaf, flower and the seed of some crop aerial parts, after different nematosises and parasitic crop, can cause all kinds of diseases of crop, and the illness causing and other disease closely similar, allow people be difficult to distinguish. According to incompletely statistics, Plant nematode causes agricultural losses to exceed 1,500 hundred million dollars every year in worldwide, and nematodiasis makes China's all kinds of crop annual production loss reach 10% ~ 15%. American-European countries generally classifies Plant nematode as agricultural " serious threat ", and South China of China is the severely afflicated area of Plant nematode. Due to physilogical characteristics and their residing environment of nematode, cause the difficulty of prevention and cure of nematode very large. The main method that adopts chemical prevention and integrated control is prevented and treated at present. Compared with traditional agricultural chemicals, new and effective, noresidue, non-harmful botanical pesticide, because its active ingredient is natural materials, are used rear innocuous substance, the environmentally safe of being easily decomposed into, to beneficial organism (being pest natural enemy) safety, the advantage such as can plant in a large number becomes the focus of research and development.
Summary of the invention
The object of the invention is to provides a kind of pesticides composition for killing nematodes containing lantadene first element and amino-oligosaccharide for the nemas of damage to crops.
The present invention in order to achieve the above object and adopt technical solution as follows:
Main active and the mass fraction thereof of composition composition pesticide of the present invention are: lantadene first element 0.1 ~ 10%, amino-oligosaccharide 0.1 ~ 10%.
Lantadene first element of the present invention is Verenaceae Lantana plant Lantana camaraLatanacamaraThe main chemical compositions of Linn, can make from the root of Lantana camara plant, stem, leaf, fruit or herb extraction separation and purification, can directly use Lantana camara plant herb or its crude extract according to actual service condition.
Amino-oligosaccharide of the present invention refers toD-Glucosamine withβThe compound sugar that-Isosorbide-5-Nitrae glycosidic bond connects, obtains after shitosan degraded again by chitin degrading and makes, or made by microorganism fermentation, is a kind of polysaccharide natural products of safe, nontoxic, noresidue, can be commercial or according to existing method preparation.
Taking above-mentioned active material as former medicine, or mix with other active component, then carry out homogeneous or mix with auxiliary agents such as carriers, can be made into aqua, missible oil, pulvis, granule, tablet etc. for preventing and treating root of the crop Root-knot.
Compared with prior art, the present invention has following beneficial effect:
1. laboratory nematicidal activity evaluation shows, lantadene first element of the present invention and amino-oligosaccharide all have to a certain degree nematocidal effect, and plain lantadene first and amino-oligosaccharide are combined and used to Meloidogyne incognitaMeloidogyneincognitaKilling action is more remarkable.
2. field control effectiveness test result shows, uses the pesticides composition for killing nematodes containing lantadene first element and amino-oligosaccharide of the present invention and can effectively prevent and treat root of the crop Root-knot, can significantly reduce the disease index of root of the crop Root-knot. For reagent thing, plant growth is had no adverse effects, without poisoning performance, particularly can promote plant growth.
3. lantadene first element of the present invention and amino-oligosaccharide are natural products, use rear easy decomposition, and noresidue, has no adverse effects to environment, can also conditioned soil loam matter.
Brief description of the drawings
Nothing.
Detailed description of the invention
Following examples are to further illustrate of the present invention, are not limitations of the present invention.
Embodiment 1: the preparation of lantadene first element
Get Lantana camara plant herb, dry rear pulverizing, sieve, take a certain amount of medicinal powder, add 2 times of volumes methanol, under room temperature, soak and extract 24h. Between soak period, frequently rock, filter, obtain methanol extract liquid and the dregs of a decoction. The dregs of a decoction repeat to extract 1 time again. Methanol extract liquid is merged, and with decolorizing with activated carbon, after decolouring, filtrate is in 50oThe following reduced pressure concentration of C reclaims methyl alcohol, obtains crude extract, and crude extract is suspended in water, and uses equal-volume chloroform extraction 2 times, and chloroform extraction liquid merges, in 50oAfter the following reduced pressure concentration of C reclaims chloroform, obtain chloroform extract, chloroform extract adds appropriate methyl alcohol low-grade fever and dissolves, in-18oC leaves standstill 24h, separates out a large amount of white crystals, and suction filtration, dries, and obtains lantadene first element, and its molecular structure is
。
Embodiment 2: lantadene first element and amino-oligosaccharide nematicidal activity evaluation
2.1 for having a try agent
L reagent: get lantadene first element that appropriate embodiment 1 makes in 50oC vacuum drying, is mixed with 1000mg/L, 500mg/L, 250mg/L solution for standby with methyl alcohol.
G reagent: get appropriate commercial amino-oligosaccharide in 50oC vacuum drying, is mixed with 1000mg/L, 500mg/L, 250mg/L solution for standby with distilled water.
2.2 for examination nematode
Be Meloidogyne incognita for examination nematodeMeloidogyneincognita. From infecting the tomato plant root of Meloidogyne incognita, picking egg capsule, egg capsule is sterilized after 0.5min with 0.2% liquor natrii hypochloritis, rushes Xian through sterilized water. Put egg capsule in sterilized water, 25 DEG C of hatchings in incubator, every 24h collects 2 instar larvaes in culture dish, and 4 DEG C of Refrigerator stores are for subsequent use.
2.3 nematicidal activity evaluation
Separately 10mL variable concentrations L reagent or G reagent being added respectively to diameter is in the culture dish of 9cm, or in culture dish, respectively add variable concentrations L reagent 5mL and variable concentrations G reagent 5mL, add again 50 μ L2000 bar/mL nematode suspension, be placed in 25 DEG C of cultivations of room temperature. The not blank of adding medicine is set, and every processing repeats 3 times. After 24h, observe the active situation of nematode, and count dead, the borer population of living of root-knot nematode. The criterion of dead worm and the worm that lives: all do not move or polypide is stiff, bending is arciform or spirality person and is judged to dead worm; All motions or polypide are S-shaped, waveform person is judged to worm alive. If judged when stiff nematode is had any problem anyway, can adopt NaOH stimulus method, in the capsule of suspecting nematode death, add the NaOH solution of a 1mol/L, if nematode health keeps motionless in 3min, be judged to be dead nematode. Calculate nemic death rate and corrected mortality.
2.4 results and analysis
Table 1 lantadene first element and amino-oligosaccharide nematicidal activity evaluation
Eelworm-killing activity result of the test is in table 1.
As seen from Table 1, lantadene first element and amino-oligosaccharide all have insecticidal activity in various degree under variable concentrations. Under identical pharmaceutical quantities, the insecticidal activity of lantadene first element is higher than amino-oligosaccharide. Under the condition that always active constituent content is constant, lantadene first element and amino-oligosaccharide are mixed to use, find that its insecticidal activity is significantly better than independent use lantadene first element or amino-oligosaccharide. Therefore, can infer, in insecticidal activity test, lantadene first element and amino-oligosaccharide have mutual promoting action. This is innovation of the present invention especially.
Embodiment 3: a kind of containing lantadene first element and amino-oligosaccharide pesticides composition for killing nematodes granule
3.1 formula compositions: lantadene first element 0.5%, amino-oligosaccharide 0.5%, Lantana camara plant medicine dregs 64%, clay 35%.
During this formula forms, lantadene first element is pressed embodiment 1 method preparation, the commercial acquisition of amino-oligosaccharide, and Lantana camara plant medicine dregs comes from embodiment 1 methyl alcohol and soaks the rear dregs of a decoction of extraction, the commercial acquisition of clay.
3.2 technical process: first lantadene first element is dissolved in to ethanol, amino-oligosaccharide is water-soluble, then the Lantana camara plant medicine dregs that dries, pulverizes and sieve and clay are placed in airtight cylinder, mix rear to the carrier spray medicine rolling, make the agent of carrier granular absorption medicine original, water becomes granule product with after ethanol volatilization, is designated hereinafter simply as LG granule.
The sick field control effectiveness test of embodiment 4:LG granule control tomato root-knot eelworm
4.1 experimental condition
4.1.1 the selection of subjects, crop and kind
Subjects is Meloidogyne incognitaMeloidogyneincognita, trial crops is tomato.
4.1.2 arable farming and environmental condition
This test is carried out in great Gang town, Panyu District of Guangzhou City, is experimental field sandy loam, and the content of organic matter is medium.
4.2 experimental design and arrangement
4.2.1 medicament
Test medicine: LG granule;
Control drug: 10% fosthiazate granule;
Being Fushan City, Guangdong Province Ying Hui crop science Co., Ltd produces.
Table 2 reagent agent experimental design
(note: A, B, C process number in active ingredient amount lantadene first element and amino-oligosaccharide respectively account for 50%. )
4.2.2 community arranges
Random district, community group arranges.
Community area and repetition: community area: 20 square metres of every communities. Number of repetition: 4 times.
4.2.3 application method
Using method: first turn over whole soil, make soil particle pine evenly thin, by treatment dosage requirement, medicament is mixed to a small amount of fine sand, early stage, spreads fertilizer over the fields in plantation ditch.
Spraying time and number of times: in the time that tomato is transplanted, dispenser once.
Prevent and treat the medicament data of other disease and pest: duration of test is not used other nematode killing agent.
4.3 investigation, record and measuring method
4.3.1 meteorology and soil information
Meteorological data: dispenser is when daily temperature 14 ~ 25oC, relative humidity 75 ~ 100%, weather is overcast to cloudy, blows slight southerly; Whole duration of test temperature 10 ~ 29oC, relative humidity 25 ~ 100%, rainfall is more early stage.
Soil information: be experimental field sandy loam, the content of organic matter is medium.
4.3.2 investigation method, time and number of times
Control time and number of times: 2 months investigation disease indexs after dispenser respectively.
4.3.3 investigation method
5 points are got in the investigation An Mei community of disease index, and 10 strain tomatoes are investigated in every 2 strains altogether, carry out classification counting, represent disease index with root knot index. The grade scale of root knot index is as table 3.
Table 3 root-knot nematode grade scale
4.3.4 drug effect computational methods
4.4 direct impacts on crop
O&E medicament has or not poisoning to crop, poisoning type and degree. Duration of test is not found poisoning phenomenon, and finding has facilitation to tomato growth.
The quantity and quality of 4.5 products
Do not investigate the quantity and quality of tomato.
4.6 impacts on other biology
4.6.1 the impact on other disease and pest
Other plant nematode kind in soil is had to inhibitory action.
4.6.2 the impact on other non-target organism
Except some nematodes of soil are had certain inhibitory action, this medicament on other non-target organism without impact.
4.7 results and analysis
The sick result of the test of table 4LG granule control tomato root-knot eelworm
Test data is analyzed (in 0.05 level and 0.01 level) by Deng Kenshi multipole poor (DMRT) method, and result of the test is in table 4. In table 4, the total active ingredient of LG granule refers to the active ingredient sum of lantadene first element and amino-oligosaccharide.
As can be seen from Table 4, use various dose LG granule and can effectively prevent and treat tomato root-knot eelworm disease, can significantly reduce the disease index of tomato root-knot eelworm disease. Investigation in 2 months after dispenser, LG granule preventive effect is 75.59% ~ 89.68%. The preventive effect of 225 grams/ha of processing of reagent agent LG granule effective ingredient consumption is slightly worse than the preventive effect of contrast medicament, but middle and high dosage reagent agent preventive effect is all higher than the preventive effect that contrasts medicament, and the preventive effect of high dose reagent agent is significantly higher than the preventive effect of low dosage reagent agent.
Observe early stage in growth, reagent agent LG granule has no adverse effects to tomato under this experimental condition, does not observe poisoning phenomenon, but LG granule chemicals treatment district tomato is obviously better than check plot tomato growing way, the sturdy prosperity of its root system, and the number of bearing fruit obviously increases.
Result of the test shows, reagent agent LG granule has good prevention effect to tomato root-knot eelworm, to not only safety but also can promote tomato growth of tomato growth, and soil soil property had to improving effect, suitablely promotes the use of aborning.
Claims (1)
1. a pesticides composition for killing nematodes, its active ingredient is made up of lantadene first element and amino-oligosaccharide.
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CN101223889A (en) * | 2008-01-31 | 2008-07-23 | 华南农业大学 | Food-intake preventer and preparing method thereof for striped flea beetle |
EP2422630A1 (en) * | 2010-08-24 | 2012-02-29 | Corn Products International, Inc. | Production of isomaltooligosaccharides and uses therefore |
CN102696693A (en) * | 2012-05-15 | 2012-10-03 | 贵阳学院 | Compound type botanical pesticide and manufacturing method and application thereof |
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CN101223889A (en) * | 2008-01-31 | 2008-07-23 | 华南农业大学 | Food-intake preventer and preparing method thereof for striped flea beetle |
EP2422630A1 (en) * | 2010-08-24 | 2012-02-29 | Corn Products International, Inc. | Production of isomaltooligosaccharides and uses therefore |
CN102696693A (en) * | 2012-05-15 | 2012-10-03 | 贵阳学院 | Compound type botanical pesticide and manufacturing method and application thereof |
Non-Patent Citations (2)
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氨基寡糖素(0.5%水剂)对黄瓜根结线虫病的防治试验;郭志刚;《农业科技通讯》;20140317;第123-124页 * |
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Denomination of invention: A Nematocidal Pesticide Combination Containing Malachitin and Aminooligosaccharides Effective date of registration: 20231229 Granted publication date: 20160518 Pledgee: China Co. truction Bank Corp Foshan branch Pledgor: FOSHAN BRIGHTMART CROP SCIENCE Co.,Ltd. Registration number: Y2023980075485 |