CN104273127A - Nematode-killing pesticide composition containing lantana allyl methyl element and amino oligosaccharins - Google Patents
Nematode-killing pesticide composition containing lantana allyl methyl element and amino oligosaccharins Download PDFInfo
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- CN104273127A CN104273127A CN201410574694.XA CN201410574694A CN104273127A CN 104273127 A CN104273127 A CN 104273127A CN 201410574694 A CN201410574694 A CN 201410574694A CN 104273127 A CN104273127 A CN 104273127A
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- 239000000575 pesticide Substances 0.000 title claims abstract description 14
- 244000075898 Lantana strigocamara Species 0.000 title abstract 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 title abstract 2
- 239000004480 active ingredient Substances 0.000 claims abstract description 6
- KCLIRHUTOPOHKJ-LSZVMECJSA-N (4r,4as,6ar,6as,6br,8ar,12ar,14bs)-2,2,6a,6b,9,9,12a-heptamethyl-4-[(z)-2-methylbut-2-enoyl]oxy-10-oxo-3,4,5,6,6a,7,8,8a,11,12,13,14b-dodecahydro-1h-picene-4a-carboxylic acid Chemical compound C([C@@]12C)CC(=O)C(C)(C)[C@@H]1CC[C@]1(C)[C@@H]2CC=C2[C@@H]3CC(C)(C)C[C@@H](OC(=O)C(\C)=C/C)[C@]3(C(O)=O)CC[C@]21C KCLIRHUTOPOHKJ-LSZVMECJSA-N 0.000 claims description 26
- KCLIRHUTOPOHKJ-DSYIDUNCSA-N Lantadene A Natural products C([C@@]12C)CC(=O)C(C)(C)[C@@H]1CC[C@]1(C)[C@@H]2CC=C2[C@@H]3CC(C)(C)C[C@@H](OC(=O)C(/C)=C/C)[C@]3(C(O)=O)CC[C@]21C KCLIRHUTOPOHKJ-DSYIDUNCSA-N 0.000 claims description 26
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- 241000244206 Nematoda Species 0.000 claims description 26
- KCLIRHUTOPOHKJ-UHFFFAOYSA-N Rehmannic acid Natural products CC12CCC(=O)C(C)(C)C1CCC1(C)C2CC=C2C3CC(C)(C)CC(OC(=O)C(C)=CC)C3(C(O)=O)CCC21C KCLIRHUTOPOHKJ-UHFFFAOYSA-N 0.000 claims description 26
- 229920001542 oligosaccharide Polymers 0.000 claims description 25
- 230000002147 killing effect Effects 0.000 claims description 6
- 201000010099 disease Diseases 0.000 abstract description 15
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 15
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- 240000003768 Solanum lycopersicum Species 0.000 description 16
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 15
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 15
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- 238000000034 method Methods 0.000 description 14
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- 240000006550 Lantana camara Species 0.000 description 7
- 230000003449 preventive effect Effects 0.000 description 7
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
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- ABUGVBRDFWGJRD-CHOYNLESSA-N [9-[(2r,3r,4s,5r)-3,4-dihydroxy-5-methyloxolan-2-yl]-2-(2,4-dinitrophenyl)sulfanylpurin-6-yl] [hydroxy(phosphonooxy)phosphoryl] hydrogen phosphate Chemical compound O[C@@H]1[C@H](O)[C@@H](C)O[C@H]1N1C2=NC(SC=3C(=CC(=CC=3)[N+]([O-])=O)[N+]([O-])=O)=NC(OP(O)(=O)OP(O)(=O)OP(O)(O)=O)=C2N=C1 ABUGVBRDFWGJRD-CHOYNLESSA-N 0.000 description 1
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- MSWZFWKMSRAUBD-QZABAPFNSA-N beta-D-glucosamine Chemical compound N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O MSWZFWKMSRAUBD-QZABAPFNSA-N 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a pesticide composition capable of preventing and treating root knot nematode diseases. The active ingredients of the pesticide composition are lantana allyl methyl element and amino oligosaccharins. The pesticide composition can be prepared into water aqua, missible oil, powder, granula and tablets for preventing and treating root knot nematode diseases. The practical application result shows that the granula prepared from the pesticide composition has remarkable prevention and control effect on root knot nematode diseases, is safe to crops, and is capable of improving the soil quality and promoting the root growth of crops.
Description
Technical field
The invention relates to a kind of pesticides composition for killing nematodes containing lantadene A prime and amino-oligosaccharide, belong to technical field of pesticide.
Background technology
Over nearly 10 years, along with the adjustment of agricultural planting structure, in China's development rapidly, the existing vegetable at protected field area of current China is more than 3,000,000 squares of hectares for Protectorate cultivation.The development of protecting field, be also that generation, the development of root-knot nematode provides adapt circumstance, after usually planting 3 ~ 5 years, the yield and quality of crop is subject to serious impact, the general underproduction 20% ~ 40%, Severe Reduction more than 60%, even has no harvest.Along with the continuation development of protecting field and the cultivation year after year of high added value crop, the problem of root-knot nematode will be more and more outstanding, and will become the key factor of serious restriction protecting field development.Nemas is the disease caused by nematode.Plant nematode, visually see and lose, main harm crop root and underground stem tuber, lepisphere stem, also the parts such as the stem of some crop field upper part, leaf, flower and seed can be endangered, all kinds of disease of crop can be caused after different nematosis and host crop, and the illness caused and other disease closely similar, allow people be difficult to distinguish.According to incompletely statistics, Plant nematode causes agricultural losses more than 1,500 hundred million dollars every year in worldwide, and nematodiasis makes China's all kinds of crop annual production loss reach 10% ~ 15%.Plant nematode is generally classified as agricultural " serious threat " by American-European countries, and South China of China is the severely afflicated area of Plant nematode.Due to the physilogical characteristics of nematode and the environment residing for them, cause the difficulty of prevention and cure of nematode very large.The main method of chemical control and integrated control that adopts is prevented and treated at present.Compared with traditional agricultural chemicals, new and effective, noresidue, non-harmful botanical pesticide due to its active ingredient be natural materials, use and be easily decomposed into innocuous substance afterwards, environmentally safe, to beneficial organism (i.e. pest natural enemy) safety, the focus that advantage becomes research and development such as can to plant in a large number.
Summary of the invention
The object of the invention is to provide a kind of pesticides composition for killing nematodes containing lantadene A prime and amino-oligosaccharide for the nemas of damage to crops.
The present invention in order to achieve the above object and adopt technical solution as follows:
The main active and the mass fraction thereof that form composition pesticide of the present invention are: lantadene A prime 0.1 ~ 10%, amino-oligosaccharide 0.1 ~ 10%.
Lantadene A prime of the present invention is Verenaceae Lantana plant Lantana camara
latana camarathe main chemical compositions of Linn, can be obtained from the root of Lantana camara plant, stem, leaf, fruit or herb extraction separation and purification, directly can use Lantana camara plant herb or its crude extract according to actual service condition.
Amino-oligosaccharide of the present invention refers to
d-Glucosamine with
βthe oligosaccharide that-Isosorbide-5-Nitrae glycosidic bond connects, is obtained after shitosan by chitin degrading that degraded is obtained again, or is obtained by fermentable, is a kind of polysaccharose natural products of safe, nontoxic, noresidue, can be commercial or prepare according to existing method.
Being former medicine with above-mentioned active substance, or mixing with other active component, then carry out homogeneous with auxiliary agents such as carriers or mix, namely can be made into aqua, missible oil, pulvis, granule, tablet etc. for preventing and treating root of the crop Root-knot.
Compared with prior art, the present invention has following beneficial effect:
1. laboratory nematicidal activity evaluation shows, lantadene A prime of the present invention and amino-oligosaccharide all have to a certain degree nematocidal effect, lantadene A prime and amino-oligosaccharide are combined and use then to Meloidogyne incognita
meloidogyne incognitakilling action is more remarkable.
2. field control effectiveness test result shows, uses the pesticides composition for killing nematodes containing lantadene A prime and amino-oligosaccharide of the present invention and can effectively prevent and treat root of the crop Root-knot, significantly can reduce the disease index of root of the crop Root-knot.For reagent thing, plant growth is had no adverse effects, without poisoning performance, particularly can promote plant growth.
3. lantadene A prime of the present invention and amino-oligosaccharide are natural products, and use rear easy decomposition, noresidue, has no adverse effects to environment, can also conditioned soil property improvement.
Accompanying drawing explanation
Nothing.
Embodiment
Following examples further illustrate of the present invention, is not limitation of the present invention.
Embodiment 1: the preparation of lantadene A prime
Get Lantana camara plant herb, dry rear pulverizing, sieve, take a certain amount of medicinal powder, add 2 times of volumes methanol, soak extraction 24h under room temperature.Frequently rock between soak period, filter, obtain methanol extract liquid and the dregs of a decoction.The dregs of a decoction repeat extraction 1 time again.Merged by methanol extract liquid, with decolorizing with activated carbon, after decolouring, filtrate is in 50
obelow C reduced pressure concentration reclaims methyl alcohol, obtains crude extract, is suspended in water by crude extract, and with equal-volume chloroform extraction 2 times, chloroform extraction liquid merges, in 50
obelow C reduced pressure concentration obtains chloroform extract after reclaiming chloroform, and chloroform extract adds proper amount of methanol low-grade fever and dissolves, in-18
oc leaves standstill 24h, separates out a large amount of white crystal, suction filtration, dries, and obtains lantadene A prime, and its molecular structure is
。
Embodiment 2: lantadene A prime and amino-oligosaccharide nematicidal activity evaluation
2.1 for having a try agent
L reagent: get the obtained lantadene A prime of appropriate embodiment 1 in 50
oc vacuum drying, is mixed with 1000mg/L, 500mg/L, 250mg/L solution for standby with methyl alcohol.
G reagent: get appropriate commercial amino-oligosaccharide in 50
oc vacuum drying, is mixed with 1000mg/L, 500mg/L, 250mg/L solution for standby with distilled water.
2.2 for examination nematode
Examination nematode is supplied to be Meloidogyne incognita
meloidogyne incognita.From the tomato plant root infecting Meloidogyne incognita, picking egg capsule, egg capsule is sterilized with 0.2% liquor natrii hypochloritis after 0.5min, rushes Xian through sterile water.Put egg capsule in sterile water, 25 DEG C of hatchings in incubator, every 24 h collect 2 instar larvaes in culture dish, and 4 DEG C of Refrigerator stores are for subsequent use.
2.3 nematicidal activity evaluation
Separately 10mL variable concentrations L reagent or G reagent being added diameter is respectively in the culture dish of 9cm, or in culture dish, respectively add variable concentrations L reagent 5mL and variable concentrations G reagent 5mL, add 50 μ L, 2000/mL nematode suspension again, be placed in room temperature 25 DEG C cultivation.The blank of not adding medicine is set, often processes repetition 3 times.Observe the active situation of nematode after 24h, and count dead, the borer population of living of root-knot nematode.Dead worm and the criterion of worm of living: allly not move or polypide is stiff, be bendingly judged to dead worm in arciform or spirality person; All motions or polypide is S-shaped, waveform person is judged to worm alive.If when judging that stiff nematode is had any problem anyway, NaOH stimulus method can be adopted, in the capsule suspecting nematode death, namely add the NaOH solution of a 1mol/L, if nematode body keeps motionless in 3min, be then judged to be dead nematode.Calculate nemic death rate and corrected mortality.
2.4 results and analysis
Table 1 lantadene A prime and amino-oligosaccharide nematicidal activity evaluation
Eelworm-killing activity result of the test is in table 1.
As seen from Table 1, lantadene A prime and amino-oligosaccharide all have insecticidal activity in various degree under variable concentrations.Under identical pharmaceutical quantities, the insecticidal activity of lantadene A prime is higher than amino-oligosaccharide.Under the condition that total active constituent content is constant, by lantadene A prime and amino-oligosaccharide used in combination, find that its insecticidal activity is significantly better than and be used alone lantadene A prime or amino-oligosaccharide.Therefore, can infer, in insecticidal activity test, lantadene A prime and amino-oligosaccharide have mutual promoting action.This is innovation of the present invention especially.
Embodiment 3: a kind of containing lantadene A prime and amino-oligosaccharide pesticides composition for killing nematodes granule
3.1 formula compositions: lantadene A prime 0.5%, amino-oligosaccharide 0.5%, Lantana camara plant medicine dregs 64%, clay 35%.
In this formula composition, lantadene A prime is by the preparation of embodiment 1 method, and the commercial acquisition of amino-oligosaccharide, Lantana camara plant medicine dregs comes from the dregs of a decoction after embodiment 1 methyl alcohol soak extraction, the commercial acquisition of clay.
3.2 technical process: first lantadene A prime is dissolved in ethanol, amino-oligosaccharide is water-soluble, then the Lantana camara plant medicine dregs drying, pulverize and sieve and clay are placed in airtight cylinder, mix the rear carrier spray medicine to rolling, carrier granular is made to adsorb medicine original agent, water becomes granule product, hereinafter referred to as LG granule with after ethanol volatilization.
The sick field control effectiveness test of embodiment 4:LG granule control tomato root-knot eelworm
4.1 experimental condition
4.1.1 the selection of subjects, crop and kind
Subjects is Meloidogyne incognita
meloidogyne incognita, trial crops is tomato.
4.1.2 crop genetics and environmental condition
This test is carried out in great Gang town, Panyu District of Guangzhou City, and be experimental field sandy loam, the content of organic matter is medium.
4.2 experimental scheme and arrangement
4.2.1 medicament
Test medicine: LG granule;
Control drug: 10% fosthiazate granule;
Be Fushan City, Guangdong Province Ying Hui crop science Co., Ltd to produce.
Table 2 reagent agent experimental scheme
(note: in A, B, C process number, active ingredient amount lantadene A prime and amino-oligosaccharide respectively account for 50%.）
4.2.2 community arranges
Random district, community group arranges.
Plot area and repetition: plot area: every 20 square metres, community.Number of repetition: 4 times.
4.2.3 application method
Using method: first turn over whole soil, makes soil particle pine evenly thin, by treatment dosage requirement, medicament is mixed a small amount of fine sand, their early stage, spread fertilizer over the fields in plantation ditch.
Spraying time and number of times: when tomato is transplanted, dispenser once.
Prevent and treat the medicament data of other damage by disease and insect: duration of test does not use other nematode killing agent.
4.3 investigation, record and measuring method
4.3.1 meteorological and soil information
Meteorological data: dispenser is when daily temperature 14 ~ 25
oc, relative moisture 75 ~ 100%, weather is overcast to cloudy, blows slight southerly; Whole duration of test temperature 10 ~ 29
oc, relative moisture 25 ~ 100%, rainfall is more for early stage.
Soil information: be experimental field sandy loam, the content of organic matter is medium.
4.3.2 investigation method, time and number of times
Control time and number of times: within 2 months, investigate disease index after administration respectively.
4.3.3 investigation method
The investigation of disease index gets 5 points by every community, and often 2 strains are investigated 10 strain tomatoes altogether, carried out classification counting, represent disease index with root knot index.The grade scale of root knot index is as table 3.
Table 3 root-knot nematode grade scale
4.3.4 drug effect computational methods
4.4 direct impacts on crop
O&E medicament to crop with or without poisoning, poisoning type and degree.Duration of test does not find poisoning phenomenon, finds there is facilitation to tomato growth.
The quantity and quality of 4.5 products
Do not investigate the quantity and quality of tomato.
4.6 impacts on other biology
4.6.1 on the impact of other damage by disease and insect
Inhibitory action is had to other plant nematode kind in soil.
4.6.2 on the impact of other non-target organism
Except having certain inhibitory action to some nematodes of soil, this medicament on other non-target organism without impact.
4.7 results and analysis
The sick result of the test of table 4 LG granule control tomato root-knot eelworm
Test data Deng Kenshi multipole difference (DMRT) method carries out analyzing (in 0.05 level and 0.01 level), and result of the test is in table 4.In table 4, the total active ingredient of LG granule refers to the active ingredient sum of lantadene A prime and amino-oligosaccharide.
As can be seen from Table 4, use various dose LG granule and can effectively prevent and treat tomato root-knot eelworm disease, significantly can reduce the disease index of tomato root-knot eelworm disease.Investigation in 2 months after dispenser, LG granule preventive effect is 75.59% ~ 89.68%.The preventive effect of reagent agent LG granule effective ingredient consumption 225 grams/ha process is slightly worse than the preventive effect of contrast medicament, but middle and high dosage reagent agent preventive effect is all higher than the preventive effect of contrast medicament, and the preventive effect of high dose reagent agent is significantly higher than the preventive effect of low dosage reagent agent.
Observe early stage in growth, reagent agent LG granule has no adverse effects to tomato under this experimental condition, does not observe poisoning phenomenon, but LG granule medicament treatment region tomato is obviously better than check plot tomato growing way, the sturdy prosperity of its root system, and number of bearing fruit obviously increases.
Result of the test shows, reagent agent LG granule has good control efficiency to tomato root-knot eelworm, to tomato growth not only safety but also can promote tomato growth, and has improving effect to soil soil property, suitablely promotes the use of aborning.
Claims (1)
1. a pesticides composition for killing nematodes, its active ingredient is made up of lantadene A prime and amino-oligosaccharide.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109824749A (en) * | 2019-01-18 | 2019-05-31 | 广西中医药大学 | Triterpenoid and its preparation method and application |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109824749A (en) * | 2019-01-18 | 2019-05-31 | 广西中医药大学 | Triterpenoid and its preparation method and application |
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Denomination of invention: A Nematocidal Pesticide Combination Containing Malachitin and Aminooligosaccharides Effective date of registration: 20231229 Granted publication date: 20160518 Pledgee: China Co. truction Bank Corp Foshan branch Pledgor: FOSHAN BRIGHTMART CROP SCIENCE Co.,Ltd. Registration number: Y2023980075485 |