CN103766140A - High-pressure blowing type liquid edible fungi production process - Google Patents
High-pressure blowing type liquid edible fungi production process Download PDFInfo
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- CN103766140A CN103766140A CN201410042594.2A CN201410042594A CN103766140A CN 103766140 A CN103766140 A CN 103766140A CN 201410042594 A CN201410042594 A CN 201410042594A CN 103766140 A CN103766140 A CN 103766140A
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Abstract
The invention relates to a high-pressure blowing type liquid edible fungi production process which comprises the following steps: preparing a culture solution; starting a microcomputer system; performing high-pressure steam sterilization; performing flame sterilization on a culture tank; performing blowing and steam-water separation; injecting fungi; performing ventilating, oxygen supplying and stirring; inoculating. The process is simple, is not influenced by an environment as being conducted in a closed manner, can greatly reduce the infection probability of a culture medium, and can be carried out in a mushroom house and a mushroom shed. According to the process, a sterilization mode of a 200-400 L high-pressure cooker is adopted for replacing sterilization modes of a large steam boiler and a sterilizer, so that the equipment investment cost is greatly reduced and the production cost is lowered; the conventional fungi production time is 3-4 months, and the fungi production time in the process is 7-10 days, so that the edible fungi production time is greatly shortened, the culture efficiency is high, the edible fungi yield is increased, the labor intensity is reduced, the production cost of mushroom farmers is lowered, and the income of the mushroom farmers is raised.
Description
technical field:
The present invention relates to a kind of edible mashroom cultivating technical field, particularly a kind of edible mushroom high pressure spray tapping system kind technique.
background technology:
At present, edible bacterium planting technology is just in developing stage, and along with the development of industrialized agriculture and agricultural modernization, planting edible mushroom output is also in continuous increase.In the domestic cultivation of the production of hybrid seeds edible mushroom, adopt asepticize operation, have complete infrastructure and good condition of culture.The production of hybrid seeds is the most important link of Edible Fungi.The quality of bacterial classification, directly has influence on output and the quality of edible mushroom.Edible fungus liquid inoculation cost is low, environmental protection, safe and can improve the level of aggregation of edible mushroom industry, has also improved greatly the quality of edible mushroom.Therefore, produce good liquid spawn and could meet the demand of enterprise, especially small-sized edible mushroom enterprise and mushroom agriculture are necessary very much.But, the bacterial classification that at present traditional seeding technique is made, workload is large, is not suitable for implant mass, and equipment is many, cost is high, is difficult for promoting; Risk is high, and traditional seeding technique sterilization effect is not good, once cause biologically active strong, large batch of medium will be infected by bacterium; The production cycle of whole liquid spawn is long, produces consuming time, consumption manpower, power consumption source, and production cost is high.
summary of the invention:
The object of this invention is to provide a kind of edible mushroom high pressure spray tapping system kind technique, can greatly reduce the probability that medium infects, shorten the bacterium time of sending out, improve edible mushroom output, thereby reduce the production cost of mushroom agriculture, improve the income of mushroom agriculture.
The present invention is achieved through the following technical solutions: a kind of edible mushroom high pressure spray tapping system kind technique, comprises the following steps:
S1: preparation culture fluid: culture fluid raw material is measured and prepared according to a certain ratio, join in 200 ~ 400L pressure cooker;
S2: open microcomputer system: corresponding data is set, debugs as automatically completing pattern;
S3: autoclaving sterilizing: enclosed high pressure pot cover, under 0.2 ~ 0.3MPa condition, culture fluid is carried out to autoclaving sterilizing 1.5 ~ 2h, sterilising temp is 126 ~ 131 ℃;
S4: culture tank flame sterilizing: 500L edible fungus species culture tank is carried out to sterilizing in tank from end opening flame;
S5: spurt carbonated drink separation: raw material good boiling sterilization in pressure cooker is spurted in electromagnetism steam-water separator from pipeline, carry out vapor-liquid separation, steam is discharged, decompression liquid flows in bacteria culture tank;
S6: inject bacterial classification: in the time that fluid temperature in bacteria culture tank drops to below 30 ℃, open in confined conditions bacterial classification injector and inject bacterial classification;
S7: ventilation oxygen-supplying stirs: after bacterial classification is injected in bacteria culture tank, starts filtrated air pump and carry out oxygen supply stirring, enter production of hybrid seeds operation 7 ~ 10 days;
S8: inoculation: after production of hybrid seeds operation, close air bleeding valve, filtrated air pump pressurization is directly inoculated with inoculating gun in culture bag.
The formula of culture fluid described in step S1 is by weight percentage: corn flour 60%, wheat bran 25%, rice bran 8.5%, wood chip 5%, gypsum 1.5%, sugar 1%.
The sterilizing of the flame of culture tank described in step S4 is to adopt alcohol fire blowing device to carry out alcohol fire blowing formula sterilizing in tank.
Liquid spawn in bacteria culture tank is in production of hybrid seeds process, and the vigor of its bacterial classification changes and growth conditions, can monitor by microcomputer system, can effectively guarantee quality and the growth rate of bacterial classification.
Beneficial effect of the present invention:
(1) technique of the present invention is simple, is all in air-tight manner, to carry out not affected by environmently, can greatly reduce the probability that medium infects, can be in mushroom room and mushroom canopy job run;
(2) in technique of the present invention, adopt the sterilizing of 200-400L pressure cooker to replace the sterilizing pattern of large-scale steam boiler and sterilizer, greatly reduce equipment investment expense, reduced production cost;
(3) traditional production of hybrid seeds time is 3 ~ 4 months, and this technique production of hybrid seeds time is 7 ~ 10 days, and the edible mashroom cultivating time shortens greatly, and culture efficiency is high, has not only improved edible mushroom output, has alleviated labour intensity, and reduces the production cost of mushroom agriculture, improves the income of mushroom agriculture.
accompanying drawing explanation:
?fig. 1 is the process chart of edible mushroom high pressure spray tapping system kind technique of the present invention.
In figure, 1-pressure cooker, 2-high-pressure pot cover, 3-blow-off valve, 4-blow-off pipe, 5-water injection pipe, 6-microcomputer system, 7-electromagnetism steam-water separator, 8-bacteria culture tank, 9-non-return valve, the manual control of 10-bacteria culture tank, 11-alcohol fire blowing device, 12-bacterial classification injector, 13-air inlet pipe, 14-filtrated air pump, 15-air bleeding valve.
embodiment:
Below in conjunction with accompanying drawing, technical scheme of the present invention is elaborated.
Embodiment 1
As shown in Figure 1, the production of hybrid seeds technique of mushroom liquid bacterial, comprises the following steps:
S1: the composition of raw materials of mushroom liquid bacterial culture fluid is pressed following percentage by weight: corn flour 60%, wheat bran 25%, rice bran 8.5%, wood chip 5%, gypsum 1.5%, sugar 1% etc. are formulated, and join in 200 ~ 400L pressure cooker 1;
S2: open microcomputer system 6, corresponding data is set, debug as automatically completing pattern;
S3: enclosed high pressure pot cover 2, under 0.2 ~ 0.3MPa condition, mushroom liquid bacterial culture fluid is carried out to autoclaving sterilizing 1.5 ~ 2h, sterilising temp is 126 ~ 131 ℃;
S4: open alcohol fire blowing device 11, from 500L bacteria culture tank 8 end openings, alcohol fire blowing formula in tank is carried out to sterilizing;
S5: open blow-off valve 3, make the interior boiling sterilization of pressure cooker good that mushroom liquid bacterial culture fluid spurts in electromagnetism steam-water separator 7 by blow-off pipe 4, carry out carbonated drink separation; Open after non-return valve 9, steam is discharged, and decompression liquid flows in bacteria culture tank 8;
S6: in the time that the fluid temperature in bacteria culture tank 8 drops to below 30 ℃, open in confined conditions bacterial classification injector 12 and inject mushroom liquid bacterial;
S7: mushroom liquid bacterial is injected in bacteria culture tank 8, starts filtrated air pump 14, carries out ventilation oxygen-supplying, stirring by air inlet pipe 13, enters production of hybrid seeds operation; During the production of hybrid seeds, carry out mushroom liquid bacterial by water injection pipe 5 and cultivate supplementing of moisture;
S8: mushroom liquid bacterial production of hybrid seeds operation, after 7 ~ 10 days, is closed air bleeding valve 15, and filtrated air pump 14 pressurizes directly inoculates with inoculating gun in culture bag.
Embodiment 2
As shown in Figure 1, the production of hybrid seeds technique of auricularia auriculajudae liquid spawn, comprises the following steps:
S1: the composition of raw materials of auricularia auriculajudae strain cultivation liquid is pressed following percentage by weight: corn flour 60%, wheat bran 25%, rice bran 8.5%, wood chip 5%, gypsum 1.5%, sugar 1% etc. are formulated, and join in 200 ~ 400L pressure cooker 1;
S2: open microcomputer system 6, corresponding data is set, debug as automatically completing pattern;
S3: enclosed high pressure pot cover 2, under 0.2 ~ 0.3MPa condition, auricularia auriculajudae strain cultivation liquid is carried out to autoclaving sterilizing 1.5 ~ 2h, sterilising temp is 126 ~ 131 ℃;
S4: open alcohol fire blowing device 11, from 500L bacteria culture tank 8 end openings, alcohol fire blowing formula in tank is carried out to sterilizing;
S5: open blow-off valve 3, make the interior boiling sterilization of pressure cooker good that auricularia auriculajudae strain cultivation liquid spurts in electromagnetism steam-water separator 7 by blow-off pipe 4, carry out carbonated drink separation; Open after non-return valve 9, steam is discharged, and decompression liquid flows in bacteria culture tank 8;
S6: in the time that the fluid temperature in bacteria culture tank 8 drops to below 30 ℃, open in confined conditions bacterial classification injector 12 and inject auricularia auriculajudae liquid spawn;
S7: auricularia auriculajudae liquid spawn is injected in bacteria culture tank 8, starts filtrated air pump 14, carries out ventilation oxygen-supplying, stirring by air inlet pipe 13, enters production of hybrid seeds operation; During the production of hybrid seeds, carry out supplementing of auricularia auriculajudae strain cultivation moisture by water injection pipe 5;
S8: auricularia auriculajudae liquid spawn production of hybrid seeds operation, after 7 ~ 10 days, is closed air bleeding valve 15, and filtrated air pump 14 pressurizes directly inoculates with inoculating gun in culture bag.
Claims (3)
1. an edible mushroom high pressure spray tapping system kind technique, comprises the following steps:
S1: preparation culture fluid: culture fluid raw material is measured and prepared according to a certain ratio, join in 200 ~ 400L pressure cooker;
S2: open microcomputer system: corresponding data is set, debugs as automatically completing pattern;
S3: autoclaving sterilizing: enclosed high pressure pot cover, under 0.2 ~ 0.3MPa condition, culture fluid is carried out to autoclaving sterilizing 1.5 ~ 2h, sterilising temp is 126 ~ 131 ℃;
S4: culture tank flame sterilizing: 500L edible fungus species culture tank is carried out to sterilizing in tank from end opening flame;
S5: spurt carbonated drink separation: raw material good boiling sterilization in pressure cooker is spurted in electromagnetism steam-water separator from pipeline, carry out vapor-liquid separation, steam is discharged, decompression liquid flows in bacteria culture tank;
S6: inject bacterial classification: in the time that fluid temperature in bacteria culture tank drops to below 30 ℃, open in confined conditions bacterial classification injector and inject bacterial classification;
S7: ventilation oxygen-supplying stirs: after bacterial classification is injected in bacteria culture tank, starts filtrated air pump and carry out oxygen supply stirring, enter production of hybrid seeds operation 7 ~ 10 days;
S8: inoculation: after production of hybrid seeds operation, close air bleeding valve, filtrated air pump pressurization is directly inoculated with inoculating gun in culture bag.
2. a kind of edible mushroom high pressure spray tapping system kind technique according to claim 1, is characterized in that: the composition of raw materials of culture fluid described in step S1 is by weight percentage: corn flour 60%, wheat bran 25%, rice bran 8.5%, wood chip 5%, gypsum 1.5%, sugar 1%.
3. a kind of edible mushroom high pressure spray tapping system kind technique according to claim 1, is characterized in that: the sterilizing of the flame of culture tank described in step S4 is to adopt alcohol fire blowing device to carry out alcohol fire blowing formula sterilizing in tank.
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| CN201410042594.2A CN103766140B (en) | 2014-01-29 | 2014-01-29 | High-pressure liquid spraying seed production process for edible mushrooms |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104003812A (en) * | 2014-06-16 | 2014-08-27 | 王晓良 | Processing method for edible fungus culture medium |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004180504A (en) * | 2002-11-29 | 2004-07-02 | Nippon Seiki Co Ltd | Method for sterilization and apparatus for sterilization each in apparatus for inoculating liquid spawn |
| KR20040077385A (en) * | 2003-02-28 | 2004-09-04 | 전민현 | Liquid mushroom spawn-cultivating device for mushroom production to shorten culture period, remove risk of bacterial contamination and enable working convenience, and consequently to improve productivity |
| CN2675680Y (en) * | 2003-12-11 | 2005-02-02 | 李吉春 | Liquid bacterial preparing bank |
| CN201517106U (en) * | 2009-07-27 | 2010-06-30 | 车斌 | Double pump alternation air feeding type liquid bacteria fermentation cylinder |
| CN202077430U (en) * | 2011-05-24 | 2011-12-21 | 湖北启明生物工程有限公司 | Fermentation tank with sterilizer at outer part |
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- 2014-01-29 CN CN201410042594.2A patent/CN103766140B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004180504A (en) * | 2002-11-29 | 2004-07-02 | Nippon Seiki Co Ltd | Method for sterilization and apparatus for sterilization each in apparatus for inoculating liquid spawn |
| KR20040077385A (en) * | 2003-02-28 | 2004-09-04 | 전민현 | Liquid mushroom spawn-cultivating device for mushroom production to shorten culture period, remove risk of bacterial contamination and enable working convenience, and consequently to improve productivity |
| CN2675680Y (en) * | 2003-12-11 | 2005-02-02 | 李吉春 | Liquid bacterial preparing bank |
| CN201517106U (en) * | 2009-07-27 | 2010-06-30 | 车斌 | Double pump alternation air feeding type liquid bacteria fermentation cylinder |
| CN202077430U (en) * | 2011-05-24 | 2011-12-21 | 湖北启明生物工程有限公司 | Fermentation tank with sterilizer at outer part |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104003812A (en) * | 2014-06-16 | 2014-08-27 | 王晓良 | Processing method for edible fungus culture medium |
| CN104003812B (en) * | 2014-06-16 | 2015-12-02 | 王晓良 | A kind of culture medium of edible fungus working method |
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