CN103749541B - Grape swelling agent and its preparation method and application - Google Patents
Grape swelling agent and its preparation method and application Download PDFInfo
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- CN103749541B CN103749541B CN201310711375.4A CN201310711375A CN103749541B CN 103749541 B CN103749541 B CN 103749541B CN 201310711375 A CN201310711375 A CN 201310711375A CN 103749541 B CN103749541 B CN 103749541B
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Abstract
The present invention relates to grape swelling agent and its preparation method and application, belong to proportion of crop planting cultivation field.Technical problem to be solved by this invention is to provide a kind of grape swelling agent and its preparation method and application.Grape swelling agent of the present invention, its active component carries out chelatropic reaction by nitrate of lime, magnesium nitrate and polyol in the water of x weight portion, and after completion of the reaction, interpolation gibberellic acid and brassin lactones obtain; Wherein, nitrate of lime 40 ~ 70 weight portion, magnesium nitrate 1 ~ 6 weight portion, polyol 10 ~ 25 weight portion; Gibberellic acid 0.04 ~ 0.4 weight portion, brassin lactones 0.0001 ~ 0.001 weight portion, x > 0; Described polyol is at least one in glycerine, ethylene glycol, mannitol, sorbierite, wood sugar alcohol and glucose.
Description
Technical field
The present invention relates to grape swelling agent and its preparation method and application, belong to proportion of crop planting cultivation field.
Background technology
In wine-growing, Fruit agent is its conventional conditioning agent, its quality that can improve output and improve grape.Fruit agent is actually a kind of plant growth promoter, has biological and chemical two kinds, and exist with fertilizer and medicament two kinds of dosage form, biological Fruit agent belongs to bioactivator, such as, and multienzyme albumen and photosynthetic enzyme etc.; The agent of chemistry Fruit belongs to hormonal substance CPPU, outstanding achievement spirit, swollen fruit dragon etc.Fruit agent can produce growth encourage, the long effect of speed to fruit.
At present, mainly orchard worker or technical staff, according to plantation experience or the single or simple hormone prescription of understanding use, use and do not have specific standards the swelling agent that orchard worker is used.The swelling agent used now mainly CPPU; CPPU is a kind of highly active phenyl urea derivatives; there is cytokine activity; have promote cell division and expansion, orga-nogenesis and protein synthesis, improve photosynthetic efficiency, strengthen resistance, delay senility, melon and fruit plant can promote flower bud differentiation, flower and fruit protecting, raising fruit-setting rate, promote the effect of Fruit.CPPU is mainly divided by irritation cell, and reaches and expand the effect of melon and fruit, but it expands effect and need to promote.
Summary of the invention
Technical problem to be solved by this invention is to provide grape swelling agent and its preparation method and application.
Grape swelling agent of the present invention, its active component carries out chelatropic reaction by nitrate of lime, magnesium nitrate and polyol in the water of x weight portion, and after completion of the reaction, interpolation gibberellic acid and brassin lactones obtain;
Wherein, nitrate of lime 40 ~ 70 weight portion, magnesium nitrate 1 ~ 6 weight portion, polyol 10 ~ 25 weight portion; Gibberellic acid 0.04 ~ 0.4 weight portion, brassin lactones 0.0001 ~ 0.001 weight portion, x > 0; Described polyol is at least one in glycerine, ethylene glycol, mannitol, sorbierite, wood sugar alcohol and glucose.
In the present invention, as long as there is the existence of a small amount of water, chelatropic reaction just can carry out, and what materially affect is the number of water consumption there is no to chelatropic reaction.But under normal circumstances, the consumption of water also there is no need more than 45 weight portions.
Gibberellic acid is gibberellin GA3.
Preferably after chelatropic reaction completes, add gibberellic acid and brassin lactones after cooling, preferably cooling adds gibberellic acid and brassin lactones below 45 DEG C.
After chelatropic reaction, brassin lactones and adding of gibberellic acid can be add after being mixed with brassin lactones by gibberellic acid again.
Further, preferred described nitrate of lime 60 ~ 70 weight portion, magnesium nitrate 2 ~ 4 weight portion, polyol 12 ~ 18 weight portion, gibberellic acid 0.07 ~ 0.11 weight portion, brassin lactones 0.0002 ~ 0.00035 weight portion.
Further, preferred described nitrate of lime 65 weight portion, magnesium nitrate 3 weight portion, polyol 15 weight portion, gibberellic acid 0.09 weight portion, brassin lactones 0.00027 weight portion.
Further, preferred described polyol is glycerine, mannitol or sorbierite; More preferably described polyol is glycerine.
Further, because gibberellic acid is not soluble, the mode of preferred described interpolation gibberellic acid and brassin lactones is: get gibberellic acid and be dissolved in the ethanol of y weight portion, then add the water of z weight portion, then add brassin lactones, adds after it dissolves again.
Described y=1.8 ~ 15, x+z=4 ~ 46 and z >=y.
Preferred described y=3 ~ 4, x+z=4 ~ 20 and z >=y.
More preferably described y=3.5, x+z=6.4 and z >=y.
Further, better expand effect to reach, after preferred chelatropic reaction except adding gibberellic acid and brassin lactones, also add auxiliary agent, described auxiliary agent is at least one in boric acid, amino acid, biochemical fulvic acid and surfactant.
Further, better expanding effect to reach, after preferred chelatropic reaction, also adding at least one in boric acid 0.05 ~ 0.4 weight portion, amino acid/11 ~ 8 weight portion, biochemical fulvic acid 0.3 ~ 1.2 weight portion and surfactant 0.5 ~ 5 weight portion.
Further, better expanding effect to reach, more preferably also adding boric acid 0.1 ~ 0.3 weight portion after chelatropic reaction, at least one in amino acid 3 ~ 5 weight portion, biochemical fulvic acid 0.6 ~ 1 weight portion and surfactant 1 ~ 3 weight portion.
Further, better expanding effect to reach, more preferably also adding boric acid 0.2 weight portion after chelatropic reaction, at least one in amino acid 4 weight portion, biochemical fulvic acid 0.8 weight portion and surfactant 2 weight portion.
Biochemical fulvic acid is application modern biotechnology, and with plant slag body for raw material, through biofermentation, produce class coalification fulvic acid material, it is a plant growth regulators, and energy Promoting plant growth, can improve plant stress-resistance, volume increase and oil recovery enhancement effect etc.
Amino acid herein can be amino acid stable for the purpose of the present invention, can be single amino acid, compound amino acid or fermentation span amino acid mother liquor.As long as containing all applicable for the amino acid whose material that system of the present invention is stable.
Further, preferred described amino acid is containing at least one in alanine, arginine, aspartic acid, asparagine, cysteine, glutamic acid, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenyl alanine, proline, serine, threonine, tryptophan, tyrosine, valine.
Further, preferred described surfactant is Tea Saponin, Tween-80, OP-10 and emulsifier 1601 at least one; More preferably described surfactant is Tea Saponin, more preferably Tea Saponin saponin content >=20wt%.
Tween-80: medicine name is polyoxyethylene sorbitan monooleate, referred to as Tween-80.
OP-10: a kind of industrial chemicals, composition is alkylphenol polyoxyethylene, white and milky pastel, soluble in water, pH value (1% water liquid): 6 ~ 7; HLB value: 14.5; Cloud point: 61 ~ 67 DEG C.
Emulsifier 1601: be again agriculture breast 1601, chemical name: phenethyl phenol polyoxyethylene poly-oxygen propylene aether, for the faint yellow mucus that flows, cloud point 76-77 DEG C, refractive index 1.4890-1.4900, adapt to water temperature range 15-35 DEG C, hydrophily is stronger, soluble in water, alcohol, benzene class equal solvent, the resistance to hard water of acid-fast alkali-proof.
Because chelatropic reaction thing is very sticky, directly by boric acid, amino acid, biochemical fulvic acid and surfactant add wherein can cause and dissolve slowly, even stir and all can not dissolve completely for 1 hour, if but first adding after water-soluble for the materials such as boric acid in chelatropic reaction thing just easily again, stirring and just can mix for 5 minutes.
Brassin lactones, gibberellic acid, boric acid, amino acid in the present invention, biochemical fulvic acid and adding of surfactant can be first gibberellic acid is dissolved in ethanol, add water hydrotropy again, and then add brassin lactones, boric acid, amino acid wherein, in the material that biochemical fulvic acid and surfactant obtain after joining chelatropic reaction again after obtaining mixture.In order to make each material fully dissolve, can be gibberellic acid is dissolved in after in ethanol adding brassin lactones, boric acid, amino acid successively again, biochemical fulvic acid and surfactant, a material after adding again after last substance dissolves.
If foam is too much in the preparation process of grape swelling agent of the present invention, can defoamer be added, as: silicone defoaming agent.
The preparation method of grape swelling agent of the present invention, comprises the steps:
(I) get nitrate of lime, magnesium nitrate and polyol and carry out chelatropic reaction in the water of x weight portion, reaction temperature is 80 ~ 120 DEG C, and the reaction time is 1 ~ 5h, reacts complete, and cooling, obtains material A;
(II) get gibberellic acid and brassin lactones, stir evenly, obtain material B;
(III) material A and material B are mixed, leave standstill, filter to get filtrate and be grape swelling agent;
Wherein, nitrate of lime 40 ~ 70 weight portion, magnesium nitrate 1 ~ 6 weight portion, polyol 10 ~ 25 weight portion; Gibberellic acid 0.04 ~ 0.4 weight portion, brassin lactones 0.0001 ~ 0.001 weight portion, x > 0; Described polyol is at least one in glycerine, ethylene glycol, mannitol, glucose, sorbierite and xylitol.
Further, preferred described nitrate of lime 60 ~ 70 weight portion, magnesium nitrate 2 ~ 4 weight portion, polyol 12 ~ 18 weight portion, gibberellic acid 0.07 ~ 0.11 weight portion, brassin lactones 0.0002 ~ 0.00035 weight portion.
Further, preferred described nitrate of lime 65 weight portion, magnesium nitrate 3 weight portion, polyol 15 weight portion, gibberellic acid 0.09 weight portion, brassin lactones 0.00027 weight portion.
Further, described in preferred steps (I), reaction temperature is 90 ~ 98 DEG C, and more preferably reaction temperature is 95 DEG C.
Owing to adopting glucose to carry out chelatropic reaction, easily to carbonize if temperature is too high, so preferable temperature is 80 ~ 85 DEG C when preferably adopting glucose to carry out chelatropic reaction.
Further, the reaction time described in preferred steps (I) is 1 ~ 3h, and more preferably the reaction time is 2h.
Further, the preparation method of material B described in preferred steps (II) gets gibberellic acid to be dissolved in the ethanol of y weight portion, then adds the water of z weight portion, then adds brassin lactones, stirs, obtains material B, described y=1.8 ~ 15, x+z=4 ~ 46 and z >=y.
Preferred described y=3 ~ 4, x+z=4 ~ 20 and z >=y.
More preferably described y=3.5, x+z=6.4 and z >=y.
Further, except gibberellic acid and brassin lactones, also add auxiliary agent in material B in preferred steps (II), described auxiliary agent is at least one in boric acid, amino acid, biochemical fulvic acid and surfactant.
Further, better expanding effect to reach, after preferred chelatropic reaction, also adding at least one in boric acid 0.05 ~ 0.4 weight portion, amino acid/11 ~ 8 weight portion, biochemical fulvic acid 0.3 ~ 1.2 weight portion and surfactant 0.5 ~ 5 weight portion.
Further, better expanding effect to reach, also adding boric acid 0.1 ~ 0.3 weight portion after preferred chelatropic reaction, at least one in amino acid 3 ~ 5 weight portion, biochemical fulvic acid 0.6 ~ 1 weight portion and surfactant 1 ~ 3 weight portion.
Further, better expanding effect to reach, also adding boric acid 0.2 weight portion after preferred chelatropic reaction, at least one in amino acid 4 weight portion, biochemical fulvic acid 0.8 weight portion and surfactant 2 weight portion.
Amino acid herein can be amino acid stable for the purpose of the present invention, can be single amino acid, compound amino acid or fermentation span amino acid mother liquor.As long as containing all applicable for the amino acid whose material that system of the present invention is stable.
Further, preferred described amino acid is containing at least one in alanine, arginine, aspartic acid, asparagine, cysteine, glutamic acid, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenyl alanine, proline, serine, threonine, tryptophan, tyrosine, valine.
Further, preferred described surfactant is Tea Saponin, Tween-80, OP-10 and emulsifier 1601 at least one; More preferably described surfactant is Tea Saponin, more preferably Tea Saponin saponin content >=20wt%.
Tween-80: medicine name is polyoxyethylene sorbitan monooleate, referred to as Tween-80.
OP-10: a kind of industrial chemicals, composition is alkylphenol polyoxyethylene, white and milky pastel, soluble in water, pH value (1% water liquid): 6 ~ 7; HLB value: 14.5; Cloud point: 61 ~ 67 DEG C.
Emulsifier 1601: be again agriculture breast 1601, chemical name: phenethyl phenol polyoxyethylene poly-oxygen propylene aether, for the faint yellow mucus that flows, cloud point 76-77 DEG C, refractive index 1.4890-1.4900, adapt to water temperature range 15-35 DEG C, hydrophily is stronger, soluble in water, alcohol, benzene class equal solvent, the resistance to hard water of acid-fast alkali-proof.
Because chelatropic reaction thing is very sticky, directly by boric acid, amino acid, biochemical fulvic acid and surfactant add wherein can cause and dissolve slowly, even stir and all can not dissolve completely for 1 hour, if but first dissolve mixing just easily carry out adding this step of chelatropic reaction thing after water-soluble for the materials such as boric acid again, stir and just can mix for 5 minutes.
Brassin lactones, gibberellic acid, boric acid, amino acid, biochemical fulvic acid and adding of surfactant can be first be dissolved in ethanol by gibberellic acid, add water hydrotropy again, and then add brassin lactones, boric acid, amino acid wherein, biochemical fulvic acid and surfactant, mixing, in the material obtained after joining chelatropic reaction again after obtaining mixture.In order to make each material fully dissolve, can be that gibberellic acid is dissolved in ethanol, then adding brassin lactones, boric acid, amino acid successively, biochemical fulvic acid and surfactant, a material after adding again after last substance dissolves.
Further, in preferred steps (II), the feed postition of auxiliary agent is add next material again after last substance dissolves.
Further, the time stirred evenly described in preferred steps (II) is 5 ~ 15 minutes; The described time stirred evenly is 7 ~ 9 minutes; The time stirred evenly is preferably 8 minutes.
Further, preferred steps (III) the described standing time is 2 ~ 48h; The preferred described standing time is 10 ~ 14h.
After chelatropic reaction, cooling, adds gibberellic acid, brassin lactones and auxiliary agent, is generally cooled to 45 DEG C can add material B.
In the process of above-mentioned preparation grape swelling agent of the present invention, if foam is too much, then need to add defoamer, adding defoamer is should agitation as appropriate, to promote that it dissolves.
The amount of above-mentioned defoamer according to foam number and determine, eliminate foam.
The application of grape swelling agent of the present invention in wine-growing.
Single usage amount when preferred grape swelling agent sprays is 25 ~ 80mL/ mu, and when more preferably grape swelling agent sprays, the usage amount of single is 30 ~ 70mL/ mu; Spray or soak fruit repeatedly, preferably 3 times.
The using method of grape swelling agent of the present invention, for measuring grape swelling agent, after dilution, sprays or soaks fruit.
The beneficial effect that the present invention has:
1, grape swelling agent of the present invention expand remarkably productive.
2, grape swelling agent of the present invention can promote crop Chlorophyll synthesis, strengthens photosynthesis, thus can increase yield.
3, grape swelling agent of the present invention is entirely water-soluble, is watered leaching fruit or spraying, easy to use.
4, grape swelling agent of the present invention can increase the content of vitamin C, soluble sugar in the size of grape fruit and single fruit weight, raising grape.
Embodiment
Technical problem to be solved by this invention is to provide grape swelling agent and its preparation method and application.
Grape swelling agent of the present invention, its active component carries out chelatropic reaction by nitrate of lime, magnesium nitrate and polyol in the water of x weight portion, and after completion of the reaction, interpolation gibberellic acid and brassin lactones obtain;
Wherein, nitrate of lime 40 ~ 70 weight portion, magnesium nitrate 1 ~ 6 weight portion, polyol 10 ~ 25 weight portion; Gibberellic acid 0.04 ~ 0.4 weight portion, brassin lactones 0.0001 ~ 0.001 weight portion, x > 0; Described polyol is at least one in glycerine, ethylene glycol, mannitol, sorbierite, wood sugar alcohol and glucose.
In the present invention, as long as there is the existence of a small amount of water, chelatropic reaction just can carry out, and what materially affect is the number of water consumption there is no to chelatropic reaction.But under normal circumstances, the consumption of water also there is no need more than 45 weight portions.
Gibberellic acid is gibberellin GA3.
Preferably after chelatropic reaction completes, add gibberellic acid and brassin lactones after cooling, preferably cooling adds gibberellic acid and brassin lactones below 45 DEG C.
After chelatropic reaction, brassin lactones and adding of gibberellic acid can be add after being mixed with brassin lactones by gibberellic acid again.
Further, preferred described nitrate of lime 60 ~ 70 weight portion, magnesium nitrate 2 ~ 4 weight portion, polyol 12 ~ 18 weight portion, gibberellic acid 0.07 ~ 0.11 weight portion, brassin lactones 0.0002 ~ 0.00035 weight portion.
Further, preferred described nitrate of lime 65 weight portion, magnesium nitrate 3 weight portion, polyol 15 weight portion, gibberellic acid 0.09 weight portion, brassin lactones 0.00027 weight portion.
Further, preferred described polyol is glycerine, mannitol or sorbierite; More preferably described polyol is glycerine.
Further, because gibberellic acid is not soluble, the mode of preferred described interpolation gibberellic acid and brassin lactones is: get gibberellic acid and be dissolved in the ethanol of y weight portion, then add the water of z weight portion, then add brassin lactones, adds after it dissolves again.
Described y=1.8 ~ 15, x+z=4 ~ 46 and z >=y.
Preferred described y=3 ~ 4, x+z=4 ~ 20 and z >=y.
More preferably described y=3.5, x+z=6.4 and z >=y.
Further, better expand effect to reach, after preferred chelatropic reaction except adding gibberellic acid and brassin lactones, also add auxiliary agent, described auxiliary agent is at least one in boric acid, amino acid, biochemical fulvic acid and surfactant.
Further, better expanding effect to reach, after preferred chelatropic reaction, also adding at least one in boric acid 0.05 ~ 0.4 weight portion, amino acid/11 ~ 8 weight portion, biochemical fulvic acid 0.3 ~ 1.2 weight portion and surfactant 0.5 ~ 5 weight portion.
Further, better expanding effect to reach, more preferably also adding boric acid 0.1 ~ 0.3 weight portion after chelatropic reaction, at least one in amino acid 3 ~ 5 weight portion, biochemical fulvic acid 0.6 ~ 1 weight portion and surfactant 1 ~ 3 weight portion.
Further, better expanding effect to reach, more preferably also adding boric acid 0.2 weight portion after chelatropic reaction, at least one in amino acid 4 weight portion, biochemical fulvic acid 0.8 weight portion and surfactant 2 weight portion.
Biochemical fulvic acid is application modern biotechnology, and with plant slag body for raw material, through biofermentation, produce class coalification fulvic acid material, it is a plant growth regulators, and energy Promoting plant growth, can improve plant stress-resistance, volume increase and oil recovery enhancement effect etc.
Amino acid herein can be amino acid stable for the purpose of the present invention, can be single amino acid, compound amino acid or fermentation span amino acid mother liquor.As long as containing all applicable for the amino acid whose material that system of the present invention is stable.
Further, preferred described amino acid is containing at least one in alanine, arginine, aspartic acid, asparagine, cysteine, glutamic acid, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenyl alanine, proline, serine, threonine, tryptophan, tyrosine, valine.
Further, preferred described surfactant is Tea Saponin, Tween-80, OP-10 and emulsifier 1601 at least one; More preferably described surfactant is Tea Saponin, more preferably Tea Saponin saponin content >=20wt%.
Tween-80: medicine name is polyoxyethylene sorbitan monooleate, referred to as Tween-80.
OP-10: a kind of industrial chemicals, composition is alkylphenol polyoxyethylene, white and milky pastel, soluble in water, pH value (1% water liquid): 6 ~ 7; HLB value: 14.5; Cloud point: 61 ~ 67 DEG C.
Emulsifier 1601: be again agriculture breast 1601, chemical name: phenethyl phenol polyoxyethylene poly-oxygen propylene aether, for the faint yellow mucus that flows, cloud point 76-77 DEG C, refractive index 1.4890-1.4900, adapt to water temperature range 15-35 DEG C, hydrophily is stronger, soluble in water, alcohol, benzene class equal solvent, the resistance to hard water of acid-fast alkali-proof.
Because chelatropic reaction thing is very sticky, directly by boric acid, amino acid, biochemical fulvic acid and surfactant add wherein can cause and dissolve slowly, even stir and all can not dissolve completely for 1 hour, if but first adding after water-soluble for the materials such as boric acid in chelatropic reaction thing just easily again, stirring and just can mix for 5 minutes.
Brassin lactones, gibberellic acid, boric acid, amino acid in the present invention, biochemical fulvic acid and adding of surfactant can be first gibberellic acid is dissolved in ethanol, add water hydrotropy again, and then add brassin lactones, boric acid, amino acid wherein, in the material that biochemical fulvic acid and surfactant obtain after joining chelatropic reaction again after obtaining mixture.In order to make each material fully dissolve, can be gibberellic acid is dissolved in after in ethanol adding brassin lactones, boric acid, amino acid successively again, biochemical fulvic acid and surfactant, a material after adding again after last substance dissolves.
If foam is too much in the preparation process of grape swelling agent of the present invention, can defoamer be added, as: silicone defoaming agent.
The preparation method of grape swelling agent of the present invention, comprises the steps:
(I) get nitrate of lime, magnesium nitrate and polyol and carry out chelatropic reaction in the water of x weight portion, reaction temperature is 80 ~ 120 DEG C, and the reaction time is 1 ~ 5h, reacts complete, and cooling, obtains material A;
(II) get gibberellic acid and brassin lactones, stir evenly, obtain material B;
(III) material A and material B are mixed, leave standstill, filter to get filtrate and be grape swelling agent;
Wherein, nitrate of lime 40 ~ 70 weight portion, magnesium nitrate 1 ~ 6 weight portion, polyol 10 ~ 25 weight portion; Gibberellic acid 0.04 ~ 0.4 weight portion, brassin lactones 0.0001 ~ 0.001 weight portion, x > 0; Described polyol is at least one in glycerine, ethylene glycol, mannitol, glucose, sorbierite and xylitol.
Further, preferred described nitrate of lime 60 ~ 70 weight portion, magnesium nitrate 2 ~ 4 weight portion, polyol 12 ~ 18 weight portion, gibberellic acid 0.07 ~ 0.11 weight portion, brassin lactones 0.0002 ~ 0.00035 weight portion.
Further, preferred described nitrate of lime 65 weight portion, magnesium nitrate 3 weight portion, polyol 15 weight portion, gibberellic acid 0.09 weight portion, brassin lactones 0.00027 weight portion.
Further, described in preferred steps (I), reaction temperature is 90 ~ 98 DEG C, and more preferably reaction temperature is 95 DEG C.
Owing to adopting glucose to carry out chelatropic reaction, easily to carbonize if temperature is too high, so preferable temperature is 80 ~ 85 DEG C when preferably adopting glucose to carry out chelatropic reaction.
Further, the reaction time described in preferred steps (I) is 1 ~ 3h, and more preferably the reaction time is 2h.
Further, the preparation method of material B described in preferred steps (II) gets gibberellic acid to be dissolved in the ethanol of y weight portion, then adds the water of z weight portion, then adds brassin lactones, stirs, obtains material B, described y=1.8 ~ 15, x+z=4 ~ 46 and z >=y.
Preferred described y=3 ~ 4, x+z=4 ~ 20 and z >=y.
More preferably described y=3.5, x+z=6.4 and z >=y.
Further, except gibberellic acid and brassin lactones, also add auxiliary agent in material B in preferred steps (II), described auxiliary agent is at least one in boric acid, amino acid, biochemical fulvic acid and surfactant.
Further, better expanding effect to reach, after preferred chelatropic reaction, also adding at least one in boric acid 0.05 ~ 0.4 weight portion, amino acid/11 ~ 8 weight portion, biochemical fulvic acid 0.3 ~ 1.2 weight portion and surfactant 0.5 ~ 5 weight portion.
Further, better expanding effect to reach, also adding boric acid 0.1 ~ 0.3 weight portion after preferred chelatropic reaction, at least one in amino acid 3 ~ 5 weight portion, biochemical fulvic acid 0.6 ~ 1 weight portion and surfactant 1 ~ 3 weight portion.
Further, better expanding effect to reach, also adding boric acid 0.2 weight portion after preferred chelatropic reaction, at least one in amino acid 4 weight portion, biochemical fulvic acid 0.8 weight portion and surfactant 2 weight portion.
Amino acid herein can be amino acid stable for the purpose of the present invention, can be single amino acid, compound amino acid or fermentation span amino acid mother liquor.As long as containing all applicable for the amino acid whose material that system of the present invention is stable.
Further, preferred described amino acid is containing at least one in alanine, arginine, aspartic acid, asparagine, cysteine, glutamic acid, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenyl alanine, proline, serine, threonine, tryptophan, tyrosine, valine.
Further, preferred described surfactant is Tea Saponin, Tween-80, OP-10 and emulsifier 1601 at least one; More preferably described surfactant is Tea Saponin, more preferably Tea Saponin saponin content >=20wt%.
Tween-80: medicine name is polyoxyethylene sorbitan monooleate, referred to as Tween-80.
OP-10: a kind of industrial chemicals, composition is alkylphenol polyoxyethylene, white and milky pastel, soluble in water, pH value (1% water liquid): 6 ~ 7; HLB value: 14.5; Cloud point: 61 ~ 67 DEG C.
Emulsifier 1601: be again agriculture breast 1601, chemical name: phenethyl phenol polyoxyethylene poly-oxygen propylene aether, for the faint yellow mucus that flows, cloud point 76-77 DEG C, refractive index 1.4890-1.4900, adapt to water temperature range 15-35 DEG C, hydrophily is stronger, soluble in water, alcohol, benzene class equal solvent, the resistance to hard water of acid-fast alkali-proof.
Because chelatropic reaction thing is very sticky, directly by boric acid, amino acid, biochemical fulvic acid and surfactant add wherein can cause and dissolve slowly, even stir and all can not dissolve completely for 1 hour, if but first dissolve mixing just easily carry out adding this step of chelatropic reaction thing after water-soluble for the materials such as boric acid again, stir and just can mix for 5 minutes.
Brassin lactones, gibberellic acid, boric acid, amino acid, biochemical fulvic acid and adding of surfactant can be first be dissolved in ethanol by gibberellic acid, add water hydrotropy again, and then add brassin lactones, boric acid, amino acid wherein, biochemical fulvic acid and surfactant, mixing, in the material obtained after joining chelatropic reaction again after obtaining mixture.In order to make each material fully dissolve, can be that gibberellic acid is dissolved in ethanol, then adding brassin lactones, boric acid, amino acid successively, biochemical fulvic acid and surfactant, a material after adding again after last substance dissolves.
Further, in preferred steps (II), the feed postition of auxiliary agent is add next material again after last substance dissolves.
Further, the time stirred evenly described in preferred steps (II) is 5 ~ 15 minutes; The described time stirred evenly is 7 ~ 9 minutes; The time stirred evenly is preferably 8 minutes.
Further, preferred steps (III) the described standing time is 2 ~ 48h; The preferred described standing time is 10 ~ 14h.
After chelatropic reaction, cooling, adds gibberellic acid, brassin lactones and auxiliary agent, is generally cooled to 45 DEG C can add material B.
In the process of above-mentioned preparation grape swelling agent of the present invention, if foam is too much, then need to add defoamer, adding defoamer is should agitation as appropriate, to promote that it dissolves.
The amount of above-mentioned defoamer according to foam number and determine, eliminate foam.
The application of grape swelling agent of the present invention in wine-growing.
Single usage amount when preferred grape swelling agent sprays is 25 ~ 80mL/ mu, and when more preferably grape swelling agent sprays, the usage amount of single is 30 ~ 70mL/ mu; Spray or soak fruit repeatedly, preferably 3 times.
The using method of grape swelling agent of the present invention, for measuring grape swelling agent, after dilution, sprays or soaks fruit.
Below in conjunction with embodiment, the specific embodiment of the present invention is further described, does not therefore limit the present invention among described scope of embodiments.
The preparation of embodiment 1 grape swelling agent
(I) get nitrate of lime 65g, magnesium nitrate 3g and glycerine 15g and be placed in Steam Heating reactor, mixing, open steam heater again, be warming up to 95 DEG C of backflows after opening condensation reflux unit, reaction 2h, reacts complete, is cooled to 45 DEG C, obtains material A;
(II) get gibberellic acid 0.09g, add after ethanol 3.5g stirs 5 minutes, then add water 6.4g, stir 5 minutes, then add brassin lactones 0.00027g successively, after it dissolves, add boric acid 0.2g again, after it dissolves, then add proline-4 g, after it dissolves, add biochemical fulvic acid 0.8g again, after it dissolves, then add Tween-80 2g, add and completely stir 8 minutes again, obtain material B;
(III) in material A, add material B, stir evenly, leave standstill 10h, filtration obtains filtrate and is grape swelling agent.
Embodiment 1 mesophytization fulvic acid purchased from Zhong Cai Sheng Hua bio tech ltd, product batch number: 20121114.
The preparation of embodiment 2 grape swelling agent
(I) get nitrate of lime 60g, magnesium nitrate 2g and ethylene glycol 18g and be placed in Steam Heating reactor, mixing, then add water 5g, open steam heater again after opening condensation reflux unit, be warming up to 90 DEG C of backflows, reaction 1h, react complete, be cooled to 45 DEG C, obtain material A;
(II) get gibberellic acid 0.11g, add ethanol 3g, stir 5 minutes, add water 15g again, stir 5 minutes, then add brassin lactones 0.0002g successively, after it dissolves, then add boric acid 0.3g, after it dissolves, add compound amino acid 5g again, after it dissolves, then add biochemical fulvic acid 0.6g, after it dissolves, add OP-101g again, add and completely stir 5 minutes again, obtain material B;
(III) in material A, add material B, stir evenly, leave standstill 12h, filtration obtains filtrate and is grape swelling agent.
Embodiment 2 mesophytization fulvic acid purchased from Zhong Cai Sheng Hua bio tech ltd, product batch number: 20121114.
In embodiment 2, compound amino acid is purchased from roc generationization Co., Ltd of city of Sichuan Mianzhu, product batch number: 20120614.
The preparation of embodiment 3 grape swelling agent
(I) get nitrate of lime 70g, magnesium nitrate 4g and ethylene glycol 12g and be placed in Steam Heating reactor, mixing, open steam heater again, be warming up to 90 DEG C of backflows after opening condensation reflux unit, reaction 1h, reacts complete, is cooled to 45 DEG C, obtains material A;
(II) get gibberellic acid 0.09g, add ethanol 4g, stir 5 minutes, add water 4g again, stir 5 minutes, then add brassin lactones 0.00035g successively, after it dissolves, then add boric acid 0.1g, after it dissolves, add glycine 4g again, after it dissolves, then add biochemical fulvic acid 1g, after it dissolves, add Tween-80 3g again, add and completely stir 5 minutes again, obtain material B;
(III) in material A, add material B, stir evenly, leave standstill 13h, filtration obtains filtrate and is grape swelling agent.
Embodiment 3 mesophytization fulvic acid purchased from Zhong Cai Sheng Hua bio tech ltd, product batch number: 20121114.
The preparation of embodiment 4 grape swelling agent
(I) get nitrate of lime 40g, magnesium nitrate 1g and xylitol 15g and be placed in Steam Heating reactor, mixing, open steam heater again, be warming up to 92 DEG C of backflows after opening condensation reflux unit, reaction 4h, reacts complete, is cooled to 45 DEG C, obtains material A;
(II) get gibberellic acid 0.2g, after adding ethanol 7.5g dissolving, then add water 8.2g, stir 5 minutes, then add brassin lactones 0.0001g successively, after it dissolves, add boric acid 0.4g again, after it dissolves, then add compound amino acid 1g, after it dissolves, add biochemical fulvic acid 0.3g again, after it dissolves, then add Tea Saponin 0.5g, add and completely stir 7 minutes again, obtain material B;
(III) in material A, add material B, stir evenly, leave standstill 8h, filtration obtains filtrate and is grape swelling agent.
Embodiment 4 mesophytization fulvic acid purchased from Zhong Cai Sheng Hua bio tech ltd, product batch number: 20121114.
In embodiment 4, compound amino acid is purchased from E Mei dragon and tiger bio tech ltd, product batch number: 20120802.
The preparation of embodiment 5 grape swelling agent
(I) get nitrate of lime 70g, magnesium nitrate 6g and glucose 10g and be placed in Steam Heating reactor, mixing, then add water 20g, open steam heater again after opening condensation reflux unit, be warming up to 82 DEG C of backflows, reaction 5h, react complete, be cooled to 45 DEG C, obtain material A;
(II) get gibberellic acid 0.04g, after adding ethanol 15g dissolving, then add water 26g, stir 8 minutes, then add brassin lactones 0.001g successively, after it dissolves, add boric acid 0.05g again, after it dissolves, then add compound amino acid 8g, after it dissolves, add biochemical fulvic acid 1.2g again, after it dissolves, then add emulsifier 16015g, add and completely stir 9 minutes again, obtain material B;
(III) in material A, add material B, stir evenly, leave standstill 14h, filtration obtains filtrate and is grape swelling agent.
Embodiment 5 mesophytization fulvic acid purchased from Zhong Cai Sheng Hua bio tech ltd, product batch number: 20121114.
In embodiment 5, compound amino acid is purchased from open-birth in morning Tetramune Co., Ltd of Sichuan Luojiang County, product batch number: 20120529.
The preparation of embodiment 6 grape swelling agent
(I) get nitrate of lime 65g, magnesium nitrate 3g and glucose 15g and be placed in Steam Heating reactor, mixing, then add water 22g, open steam heater again after opening condensation reflux unit, be warming up to 82 DEG C of backflows, reaction 5h, react complete, be cooled to 45 DEG C, obtain material A;
(II) get gibberellic acid 0.09g and be dissolved in 3.5g ethanol, then add water 20g, stir 7 minutes, then add brassin lactones 0.00027g, stir 8 minutes, obtain material B;
(III) in material A, add material B, stir evenly, leave standstill 16h, filtration obtains filtrate and is grape swelling agent.
The preparation of embodiment 7 grape swelling agent
(I) get nitrate of lime 70g, magnesium nitrate 6g and glycerine 10g and be placed in Steam Heating reactor, mixing, open steam heater again, be warming up to 92 DEG C of backflows after opening condensation reflux unit, reaction 5h, reacts complete, is cooled to 45 DEG C, obtains material A;
(II) get gibberellic acid 0.04g and be dissolved in 15g ethanol, then add water 20g, then add brassin lactones 0.00027g wherein, treat that it dissolves, then add boric acid 0.3g wherein, stir 9 minutes, obtain material B;
(III) in material A, add material B, stir evenly, leave standstill 10h, filtration obtains filtrate and is grape swelling agent.
The preparation of embodiment 8 grape swelling agent
(I) get nitrate of lime 45g, magnesium nitrate 3g and mannitol 15g and be placed in Steam Heating reactor, mixing, open steam heater again, be warming up to 92 DEG C of backflows after opening condensation reflux unit, reaction 3h, reacts complete, is cooled to 45 DEG C, obtains material A;
(II) get gibberellic acid 0.4g and be dissolved in 15g ethanol, then add water 20g, then add brassin lactones 0.00015g wherein, treat that it dissolves, then add boric acid 0.4g, after it dissolves, add compound amino acid 8g again, stir 10 minutes, obtain material B;
(III) in material A, add material B, stir evenly, leave standstill 18h, filtration obtains filtrate and is grape swelling agent.
In embodiment 8, compound amino acid is purchased from open-birth in morning Tetramune Co., Ltd of Sichuan Luojiang County, product batch number: 20120529.
The preparation of embodiment 9 grape swelling agent
(I) get nitrate of lime 55g, magnesium nitrate 2g and sorbierite 22g and be placed in Steam Heating reactor, mixing, then add water 10g, open steam heater again after opening condensation reflux unit, be warming up to 92 DEG C of backflows, reaction 4h, react complete, be cooled to 45 DEG C, obtain material A;
(II) get gibberellic acid 0.4g and be dissolved in 15g ethanol, then add water 15g, stir 10 minutes, add brassin lactones 0.00026g more wherein and treat that it dissolves, then add biochemical fulvic acid 1.2g, after it dissolves, add emulsifier 16015g again, stir 5 minutes, obtain material B;
(III) in material A, add material B, stir evenly, leave standstill 10h, filtration obtains filtrate and is grape swelling agent.
Embodiment 9 mesophytization fulvic acid purchased from Zhong Cai Sheng Hua bio tech ltd, product batch number: 20121114.
The preparation of embodiment 10 grape swelling agent
(I) get nitrate of lime 48g, magnesium nitrate 4g and glycerine 13g and be placed in Steam Heating reactor, mixing, open steam heater again, be warming up to 92 DEG C of backflows after opening condensation reflux unit, reaction 5h, reacts complete, is cooled to 45 DEG C, obtains material A;
(II) get gibberellic acid 0.3g and be dissolved in 10g ethanol, then add water 12g, stir 5 minutes, then add brassin lactones 0.0003g wherein, treat that it dissolves, then boric acid 0.2g wherein, treat that it dissolves, then add Tea Saponin 5g, stir 7 minutes, obtain material B;
(III) in material A, add material B, stir evenly, leave standstill 12h, filtration obtains filtrate and is grape swelling agent.
The preparation method of the brassin lactones described in embodiment 1 ~ 10 is that the patent system of 201310063327.9 is for obtaining according to application number.
Test of pesticide effectiveness part:
Reagent agent is the grape swelling agent that embodiment 1 ~ 10 obtains, 0.1% CPPU soluble liquid and clear water.
The preparation of medicament is in table 1.
The preparation of table 1 medicament
In table 1,0.1% CPPU soluble liquid is bought from Sichuan Lanyue Science & Technology Co., Ltd., lot number: 20120922.
1, supplying to study thing is kyoto grape
This test grape swelling agent designs 2 concentration, and contrast medicament only uses to be recommended best working concentration to carry out leaching fruit namely to get 0.1% CPPU soluble liquid 15mL and dilute 100 times, if clear water blank group.Select a kyoto grape planting base, be divided into 22 communities, random label is 1 ~ 22, and every plot area is 20m
2, the medicament prepared in table 1 is used according to numbering and community label one_to_one corresponding.The first time Dressing date of grape swelling agent is the kyoto grape physiology fallen flowers phase, use three times, second time dispenser and for the first time dispenser interval 14 days, third time dispenser and for the second time dispenser interval 14 days, in whole process of the test, all process communities adopt same management method.
Sampling when kyoto grape is ripe, detect single fruit weight, Vitamin C content, soluble sugar content, the standard of sampling is random sampling, gets 25 string kyoto grapes and detects, and single fruit weight is the mean value calculated.
After kyoto grape detects, parameters is in table 2.
The parameters of table 2 kyoto grape
| Numbering | Single fruit weight (g) | Vc content (mg/100g) | Soluble sugar content (wt%) |
| 1 | 8.53 | 4.77 | 14.21 |
| 2 | 8.57 | 4.79 | 14.33 |
| 3 | 8.50 | 4.71 | 13.70 |
| 4 | 8.52 | 4.74 | 13.68 |
| 5 | 8.48 | 4.69 | 14.15 |
| 6 | 8.49 | 4.73 | 14.22 |
| 7 | 8.45 | 4.65 | 14.05 |
| 8 | 8.47 | 4.69 | 14.10 |
| 9 | 8.41 | 4.63 | 14.06 |
| 10 | 8.46 | 4.70 | 14.11 |
| 11 | 8.27 | 4.51 | 14.01 |
| 12 | 8.31 | 4.53 | 14.07 |
| 13 | 8.29 | 4.52 | 14.03 |
| 14 | 8.34 | 4.55 | 13.89 |
| 15 | 8.31 | 4.53 | 14.04 |
| 16 | 8.33 | 4.57 | 14.09 |
| 17 | 8.29 | 4.53 | 14.06 |
| 18 | 8.35 | 4.59 | 14.11 |
| 19 | 8.34 | 4.55 | 13.77 |
| 20 | 8.38 | 4.62 | 14.13 |
| 21 | 7.84 | 4.23 | 13.43 |
| 22 | 7.57 | 3.94 | 13.56 |
Application of the kyoto grape single fruit weight of grape swelling agent of the present invention, Vc content, soluble sugar content as can be seen from Table 2 all higher than the kyoto grape of the grape and clear water blank group of using CPPU.
2, supplying to study thing is Qarnet grape
This test grape swelling agent designs 2 concentration, and contrast medicament only uses to be recommended best working concentration to carry out leaching fruit namely to get 0.1% CPPU soluble liquid 15mL and dilute 100 times, if clear water blank group.Select a Qarnet grape-planting regions, be divided into 22 communities, random label is 1 ~ 22, and every plot area is 20m
2, the medicament prepared in table 1 is used according to numbering and community label one_to_one corresponding.The first time Dressing date of grape swelling agent is the Qarnet grape physiology fallen flowers phase, use three times, second time dispenser and for the first time dispenser interval 14 days, third time dispenser and for the second time dispenser interval 14 days, in whole process of the test, all process communities adopt same management method.
Sample when Qarnet grape maturity, detect single fruit weight, Vitamin C content, soluble sugar content, the standard of sampling is random sampling, gets 25 string Qarnet grapes and detects, and single fruit weight is the mean value calculated.
After Qarnet grape detects, parameters is in table 3.
The parameters of table 3 Qarnet grape
| Numbering | Single fruit weight (g) | Vc content (mg/100g) | Soluble sugar content (wt%) |
| 1 | 8.57 | 4.79 | 14.33 |
| 2 | 8.61 | 4.83 | 13.80 |
| 3 | 8.54 | 4.74 | 14.25 |
| 4 | 8.57 | 4.78 | 14.29 |
| 5 | 8.52 | 4.71 | 14.27 |
| 6 | 8.56 | 4.76 | 14.31 |
| 7 | 8.48 | 4.68 | 14.21 |
| 8 | 8.51 | 4.72 | 14.29 |
| 9 | 8.45 | 4.64 | 14.20 |
| 10 | 8.49 | 4.71 | 13.77 |
| 11 | 8.32 | 4.52 | 14.17 |
| 12 | 8.37 | 4.59 | 14.24 |
| 13 | 8.34 | 4.53 | 14.16 |
| 14 | 8.37 | 4.61 | 14.25 |
| 15 | 8.35 | 4.52 | 14.18 |
| 16 | 8.38 | 4.57 | 14.25 |
| 17 | 8.33 | 4.59 | 14.17 |
| 18 | 8.41 | 4.63 | 13.84 |
| 19 | 8.37 | 4.57 | 14.20 |
| 20 | 8.45 | 4.65 | 14.25 |
| 21 | 7.94 | 3.92 | 13.20 |
| 22 | 7.62 | 4.01 | 13.43 |
Application of the Qarnet grape single fruit weight of grape swelling agent of the present invention, Vc content, soluble sugar content as can be seen from Table 3 all higher than the Qarnet grape using CPPU and clear water blank group.
3, supplying to study thing is that rattan harvests grape
This test grape swelling agent designs 2 concentration, and contrast medicament only uses to be recommended best working concentration to carry out leaching fruit namely to get 0.1% CPPU soluble liquid 15mL and dilute 100 times, if clear water blank group.Select a rattan and harvest grape-planting regions, be divided into 22 communities, random label is 1 ~ 22, and every plot area is 20m
2, the medicament prepared in table 1 is used according to numbering and community label one_to_one corresponding.The first time Dressing date of grape swelling agent is that rattan harvests the grape physiology fallen flowers phase, use three times, second time dispenser and for the first time dispenser interval 14 days, third time dispenser and for the second time dispenser interval 14 days, in whole process of the test, all process communities adopt same management method.
Sample when rattan harvests grape maturity, detect single fruit weight, Vitamin C content, soluble sugar content, the standard of sampling is random sampling, gets 25 string rattans and harvests grape and detect, and single fruit weight is the mean value calculated.
Rattan is harvested grape and detects rear parameters in table 4.
Table 4 rattan harvests the parameters of grape
| Numbering | Single fruit weight (g) | Vc content (mg/100g) | Soluble sugar content (wt%) |
| 1 | 10.38 | 4.68 | 13.93 |
| 2 | 10.41 | 4.71 | 14.28 |
| 3 | 10.33 | 4.63 | 14.21 |
| 4 | 10.37 | 4.65 | 14.22 |
| 5 | 10.32 | 4.61 | 14.19 |
| 6 | 10.35 | 4.67 | 14.21 |
| 7 | 10.28 | 4.57 | 14.14 |
| 8 | 10.30 | 4.61 | 13.78 |
| 9 | 10.24 | 4.53 | 14.15 |
| 10 | 10.29 | 4.59 | 14.19 |
| 11 | 10.18 | 4.47 | 14.12 |
| 12 | 10.22 | 4.51 | 14.15 |
| 13 | 10.21 | 4.49 | 14.13 |
| 14 | 10.24 | 4.53 | 14.17 |
| 15 | 10.20 | 4.51 | 14.11 |
| 16 | 10.26 | 4.54 | 13.68 |
| 17 | 10.22 | 4.50 | 14.13 |
| 18 | 10.25 | 4.55 | 14.19 |
| 19 | 10.24 | 4.52 | 14.15 |
| 20 | 10.27 | 4.55 | 14.18 |
| 21 | 9.68 | 3.68 | 13.13 |
| 22 | 9.45 | 3.51 | 13.41 |
The rattan that application of grape swelling agent of the present invention is as can be seen from Table 4 harvested grape single fruit weight, Vc content, soluble sugar content and all harvests grape higher than the rattan of using CPPU and clear water blank group.
4, supplying to study thing is Manicure Finger
This test grape swelling agent designs 2 concentration, and contrast medicament only uses to be recommended best working concentration to carry out leaching fruit namely to get 0.1% CPPU soluble liquid 15mL and dilute 100 times, if clear water blank group.Select a Manicure Finger planting base, be divided into 22 communities, random label is 1 ~ 22, and every plot area is 20m
2, the medicament prepared in table 1 is used according to numbering and community label one_to_one corresponding.The first time Dressing date of grape swelling agent is the Manicure Finger physiology fallen flowers phase, use three times, second time dispenser and for the first time dispenser interval 14 days, third time dispenser and for the second time dispenser interval 14 days, in whole process of the test, all process communities adopt same management method.
Sampling when Manicure Finger is ripe, detect single fruit weight, Vitamin C content, soluble sugar content, the standard of sampling is random sampling, gets 25 string Manicure Finger and detects, and single fruit weight is the mean value calculated.
After Manicure Finger detects, parameters is in table 5.
The parameters of table 5 Manicure Finger
| Numbering | Single fruit weight (g) | Vc content (mg/100g) | Soluble sugar content (wt%) |
| 1 | 13.56 | 5.67 | 14.47 |
| 2 | 13.77 | 5.70 | 13.53 |
| 3 | 13.48 | 5.63 | 14.43 |
| 4 | 13.53 | 5.66 | 14.49 |
| 5 | 13.49 | 5.63 | 14.43 |
| 6 | 13.51 | 5.67 | 13.60 |
| 7 | 13.43 | 5.55 | 14.38 |
| 8 | 13.48 | 5.61 | 13.64 |
| 9 | 13.45 | 4.54 | 13.35 |
| 10 | 13.50 | 5.58 | 14.43 |
| 11 | 13.34 | 5.42 | 14.32 |
| 12 | 13.37 | 5.48 | 14.37 |
| 13 | 13.36 | 5.45 | 14.35 |
| 14 | 13.40 | 5.51 | 13.51 |
| 15 | 13.38 | 5.46 | 14.32 |
| 16 | 13.42 | 5.49 | 14.38 |
| 17 | 13.40 | 5.48 | 14.36 |
| 18 | 13.43 | 5.53 | 13.42 |
| 19 | 13.45 | 4.51 | 14.39 |
| 20 | 13.49 | 5.57 | 14.41 |
| 21 | 12.88 | 4.16 | 13.09 |
| 22 | 12.46 | 3.86 | 12.87 |
Application of the Manicure Finger single fruit weight of grape swelling agent of the present invention, Vc content, soluble sugar content as can be seen from Table 5 all higher than the Manicure Finger using CPPU and clear water blank group.
In effect experiment part, ascorbic assay method measures with reference to the determination method (2,6-dichloroindophenol titration) of GB/T6195-1986 fruit, vegetables Vitamin C content.
In effect experiment part, the assay method of soluble sugar is with reference to the mensuration copper reduction iodometric determination of soluble sugar in NY/T1278-2007 vegetables and goods thereof.
Claims (43)
1. grape swelling agent, is characterized in that: its active component carries out chelatropic reaction by nitrate of lime, magnesium nitrate and polyol in the water of x weight portion, and after completion of the reaction, interpolation gibberellic acid and brassin lactones obtain;
Wherein, nitrate of lime 40 ~ 70 weight portion, magnesium nitrate 1 ~ 6 weight portion, polyol 10 ~ 25 weight portion; Gibberellic acid 0.04 ~ 0.4 weight portion, brassin lactones 0.0001 ~ 0.001 weight portion, x > 0; Described polyol is at least one in glycerine, ethylene glycol, mannitol, sorbierite, wood sugar alcohol and glucose.
2. grape swelling agent according to claim 1, it is characterized in that: described nitrate of lime 60 ~ 70 weight portion, magnesium nitrate 2 ~ 4 weight portion, polyol 12 ~ 18 weight portion, gibberellic acid 0.07 ~ 0.11 weight portion, brassin lactones 0.0002 ~ 0.00035 weight portion.
3. grape swelling agent according to claim 2, is characterized in that: described nitrate of lime 65 weight portion, magnesium nitrate 3 weight portion, polyol 15 weight portion, gibberellic acid 0.09 weight portion, brassin lactones 0.00027 weight portion.
4. the grape swelling agent according to any one of claims 1 to 3, is characterized in that: described polyol is glycerine, mannitol or sorbierite.
5. grape swelling agent according to claim 4, is characterized in that: described polyol is glycerine.
6. the grape swelling agent according to any one of claims 1 to 3, it is characterized in that: the mode of described interpolation gibberellic acid and brassin lactones is: get gibberellic acid and be dissolved in the ethanol of y weight portion, add the water of z weight portion again, then add brassin lactones, add again after it dissolves; Described y=1.8 ~ 15, x+z=4 ~ 46 and z >=y.
7. grape swelling agent according to claim 4, it is characterized in that: the mode of described interpolation gibberellic acid and brassin lactones is: get gibberellic acid and be dissolved in the ethanol of y weight portion, add the water of z weight portion again, then add brassin lactones, add again after it dissolves; Described y=1.8 ~ 15, x+z=4 ~ 46 and z >=y.
8. grape swelling agent according to claim 5, it is characterized in that: the mode of described interpolation gibberellic acid and brassin lactones is: get gibberellic acid and be dissolved in the ethanol of y weight portion, add the water of z weight portion again, then add brassin lactones, add again after it dissolves; Described y=1.8 ~ 15, x+z=4 ~ 46 and z >=y.
9. grape swelling agent according to claim 6, is characterized in that: described gibberellic acid and brassin lactones are dissolved in the ethanol of y weight portion and the water of z weight portion; Described y=3 ~ 4, x+z=4 ~ 20 and z >=y.
10. grape swelling agent according to claim 7, is characterized in that: described gibberellic acid and brassin lactones are dissolved in the ethanol of y weight portion and the water of z weight portion; Described y=3 ~ 4, x+z=4 ~ 20 and z >=y.
11. grape swelling agents according to claim 8, is characterized in that: described gibberellic acid and brassin lactones are dissolved in the ethanol of y weight portion and the water of z weight portion; Described y=3 ~ 4, x+z=4 ~ 20 and z >=y.
12. grape swelling agents according to claim 9, is characterized in that: described gibberellic acid and brassin lactones are dissolved in the ethanol of y weight portion and the water of z weight portion; Described y=3.5, x+z=6.4 and z >=y.
13. grape swelling agents according to any one of claims 1 to 3, it is characterized in that: after chelatropic reaction except interpolation gibberellic acid and brassin lactones, also add auxiliary agent, described auxiliary agent is at least one in boric acid, amino acid, biochemical fulvic acid and surfactant; Described boric acid 0.05 ~ 0.4 weight portion, amino acid/11 ~ 8 weight portion, biochemical fulvic acid 0.3 ~ 1.2 weight portion and surfactant 0.5 ~ 5 weight portion.
14. grape swelling agents according to claim 4, is characterized in that: after chelatropic reaction except interpolation gibberellic acid and brassin lactones, also add auxiliary agent, described auxiliary agent is at least one in boric acid, amino acid, biochemical fulvic acid and surfactant; Described boric acid 0.05 ~ 0.4 weight portion, amino acid/11 ~ 8 weight portion, biochemical fulvic acid 0.3 ~ 1.2 weight portion and surfactant 0.5 ~ 5 weight portion.
15. grape swelling agents according to claim 13, is characterized in that: described boric acid 0.1 ~ 0.3 weight portion, amino acid 3 ~ 5 weight portion, biochemical fulvic acid 0.6 ~ 1 weight portion and surfactant 1 ~ 3 weight portion.
16. grape swelling agents according to claim 14, is characterized in that: described boric acid 0.1 ~ 0.3 weight portion, amino acid 3 ~ 5 weight portion, biochemical fulvic acid 0.6 ~ 1 weight portion and surfactant 1 ~ 3 weight portion.
17. grape swelling agents according to claim 15, is characterized in that: described boric acid 0.2 weight portion, amino acid 4 weight portion, biochemical fulvic acid 0.8 weight portion and surfactant 2 weight portion.
18. grape swelling agents according to claim 16, is characterized in that: described boric acid 0.2 weight portion, amino acid 4 weight portion, biochemical fulvic acid 0.8 weight portion and surfactant 2 weight portion.
19. grape swelling agents according to claim 13, is characterized in that: described amino acid is containing at least one in alanine, arginine, aspartic acid, asparagine, cysteine, glutamic acid, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenyl alanine, proline, serine, threonine, tryptophan, tyrosine, valine; Described surfactant is Tea Saponin, Tween-80, OP-10 and emulsifier 1601 at least one.
20. grape swelling agents according to claim 14, is characterized in that: described amino acid is containing at least one in alanine, arginine, aspartic acid, asparagine, cysteine, glutamic acid, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenyl alanine, proline, serine, threonine, tryptophan, tyrosine, valine; Described surfactant is Tea Saponin, Tween-80, OP-10 and emulsifier 1601 at least one.
21. grape swelling agents according to claim 15, is characterized in that: described amino acid is containing at least one in alanine, arginine, aspartic acid, asparagine, cysteine, glutamic acid, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenyl alanine, proline, serine, threonine, tryptophan, tyrosine, valine; Described surfactant is Tea Saponin, Tween-80, OP-10 and emulsifier 1601 at least one.
22. grape swelling agents according to claim 16, is characterized in that: described amino acid is containing at least one in alanine, arginine, aspartic acid, asparagine, cysteine, glutamic acid, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenyl alanine, proline, serine, threonine, tryptophan, tyrosine, valine; Described surfactant is Tea Saponin, Tween-80, OP-10 and emulsifier 1601 at least one.
23. grape swelling agents according to claim 17, is characterized in that: described amino acid is containing at least one in alanine, arginine, aspartic acid, asparagine, cysteine, glutamic acid, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenyl alanine, proline, serine, threonine, tryptophan, tyrosine, valine; Described surfactant is Tea Saponin, Tween-80, OP-10 and emulsifier 1601 at least one.
24. grape swelling agents according to claim 18, is characterized in that: described amino acid is containing at least one in alanine, arginine, aspartic acid, asparagine, cysteine, glutamic acid, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenyl alanine, proline, serine, threonine, tryptophan, tyrosine, valine; Described surfactant is Tea Saponin, Tween-80, OP-10 and emulsifier 1601 at least one.
25. grape swelling agents according to claim 19, is characterized in that: described surfactant is Tea Saponin.
26. grape swelling agents according to claim 20, is characterized in that: described surfactant is Tea Saponin.
27. grape swelling agents according to claim 21, is characterized in that: described surfactant is Tea Saponin.
28. grape swelling agents according to claim 22, is characterized in that: described surfactant is Tea Saponin.
29. grape swelling agents according to claim 23, is characterized in that: described surfactant is Tea Saponin.
30. grape swelling agents according to claim 24, is characterized in that: described surfactant is Tea Saponin.
31. grape swelling agents according to claim 25, is characterized in that: described surfactant Tea Saponin saponin content >=20wt%.
32. grape swelling agents according to claim 26, is characterized in that: described surfactant Tea Saponin saponin content >=20wt%.
33. grape swelling agents according to claim 27, is characterized in that: described surfactant Tea Saponin saponin content >=20wt%.
34. grape swelling agents according to claim 28, is characterized in that: described surfactant Tea Saponin saponin content >=20wt%.
35. grape swelling agents according to claim 29, is characterized in that: described surfactant Tea Saponin saponin content >=20wt%.
36. grape swelling agents according to claim 30, is characterized in that: described surfactant Tea Saponin saponin content >=20wt%.
The preparation method of grape swelling agent described in 37. claims 1, is characterized in that: comprise the steps:
(I) get nitrate of lime, magnesium nitrate and polyol and carry out chelatropic reaction in the water of x weight portion, reaction temperature is 80 ~ 120 DEG C, and the reaction time is 1 ~ 5h, reacts complete, and cooling, obtains material A;
(II) get gibberellic acid and brassin lactones, stir evenly, obtain material B;
(III) material A and material B are mixed, leave standstill, filter to get filtrate and be grape swelling agent;
Wherein, nitrate of lime 40 ~ 70 weight portion, magnesium nitrate 1 ~ 6 weight portion, polyol 10 ~ 25 weight portion; Gibberellic acid 0.04 ~ 0.4 weight portion, brassin lactones 0.0001 ~ 0.001 weight portion, x > 0; Described polyol is at least one in glycerine, ethylene glycol, mannitol, glucose, sorbierite and xylitol.
38., according to the preparation method of grape swelling agent according to claim 37, is characterized in that: described in step (I), reaction temperature is 90 ~ 100 DEG C.
39., according to the preparation method of grape swelling agent according to claim 38, is characterized in that: described in step (I), reaction temperature is 93 ~ 97 DEG C.
40. according to the preparation method of grape swelling agent according to claim 37, and it is characterized in that: described polyol is glucose, described reaction temperature is 80 ~ 85 DEG C.
41., according to the preparation method of grape swelling agent according to claim 37, is characterized in that: the reaction time described in step (I) is 1.5 ~ 3.5h;
The preparation method of material B described in step (II) gets gibberellic acid to be dissolved in the ethanol of y weight portion, then adds the water of z weight portion, then adds brassin lactones, stirs, obtains material B;
Described y=1.8 ~ 15, x+z=4 ~ 46 and z >=y;
Except adding gibberellic acid and brassin lactones, also add auxiliary agent in step (II), described auxiliary agent is at least one in boric acid, amino acid, biochemical fulvic acid and surfactant; The feed postition of described auxiliary agent is add next material again after last substance dissolves.
The preparation method of 42. grape swelling agents according to claim 41, is characterized in that: the reaction time described in step (I) is 2h.
The application of grape swelling agent in viticulture described in 43. any one of claims 1 to 36.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310711375.4A CN103749541B (en) | 2013-12-20 | 2013-12-20 | Grape swelling agent and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310711375.4A CN103749541B (en) | 2013-12-20 | 2013-12-20 | Grape swelling agent and its preparation method and application |
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| CN104447041B (en) * | 2014-12-16 | 2017-08-29 | 成都新朝阳作物科学有限公司 | Kiwi berry liquid expands Resource and its preparation method and application |
| CN104996448A (en) * | 2015-07-29 | 2015-10-28 | 句容市茅山镇恒泰家庭农场 | Big fruit culture agent for kyoho grape and preparing method and application of big fruit culture agent |
| CN105076253B (en) * | 2015-08-26 | 2018-01-09 | 山东省农业科学院农业质量标准与检测技术研究所 | A kind of cucumber specific complex type growth regulator |
| CN106069565A (en) * | 2016-06-22 | 2016-11-09 | 马鞍山市国华农业发展有限公司 | A kind of breeding method of currant |
| CN108990684B (en) * | 2018-09-04 | 2021-01-12 | 山东省果树研究所 | Grape cultivation method suitable for non-buried cold-proof area |
| CN110074114A (en) * | 2019-04-29 | 2019-08-02 | 天津市汉邦植物保护剂有限责任公司 | A kind of brassinolide-containing, aqua of sugar alcohol calcium and preparation method and application |
| CN113598171B (en) * | 2021-08-27 | 2023-07-18 | 江苏省农业科学院 | Melon and fruit vegetable fruit swelling agent and application thereof |
| CN115152767B (en) * | 2022-08-19 | 2024-03-15 | 硅羿科技（上海）有限公司 | Pesticide preparation containing 24-epibrassinolide and gibberellic acid, and preparation method and application thereof |
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