CN103734745B - Method for preparing compound microcapsule of high-concentration lactoferrin and lactobacillus acidophilus - Google Patents

Method for preparing compound microcapsule of high-concentration lactoferrin and lactobacillus acidophilus Download PDF

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CN103734745B
CN103734745B CN201310693004.8A CN201310693004A CN103734745B CN 103734745 B CN103734745 B CN 103734745B CN 201310693004 A CN201310693004 A CN 201310693004A CN 103734745 B CN103734745 B CN 103734745B
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lactoferrin
concentration
lactobacillus acidophilus
capsule
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CN103734745A (en
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朱玲
刘敏
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Wuhan Yuanda Biological Science & Technology Co Ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/19—Dairy proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
    • A23P10/00—Shaping or working of foodstuffs characterised by the products
    • A23P10/30—Encapsulation of particles, e.g. foodstuff additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/113—Acidophilus

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Mycology (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicinal Preparation (AREA)

Abstract

The invention relates to a method for preparing a compound microcapsule of high-concentration lactoferrin and lactobacillus acidophilus. The method comprises the following steps: centrifuging to remove fat, eliminating casein by an isoelectric point precipitation method, sterilizing by a micro-filtration membrane, performing ultrafiltration and concentration, inoculating Lactobacillus acidophilus, adding emulgator to homogenize, concentrating in vacuum, slowly adding a protein concentrated solution into a dissolved wall material, adding a freeze-drying protective additive, freezing and drying. The compound microcapsule prepared by the method is high in content of lactoferrin, and the purity of the lactoferrin is about 48%; compared with the prior art, the compound microcapsule alleviates the decomposition of heat sensitive components in the lactoferrin structure and improves the yield of the lactoferrin; meanwhile, the compound microcapsule prepared by the method has the advantages that the resistance to the ambient environment is enhanced, the activity loss of lactoferrin in the processing and transportation process and in human body gastric acid is reduced, the physiological activity of lactoferrin and lactobacillus acidophilus reaching intestinal canals is remarkably improved, double activities of immunity and micro-ecology are effectively maintained, the healthcare function of the microcapsule to human bodies is enhanced, and the additional values of the microcapsule are increased.

Description

The preparation method of a kind of high-concentration lactoferrin and lactobacillus acidophilus composite micro-capsule
Technical field
The present invention relates to health food manufacture field, be specifically related to the preparation method of a kind of high-concentration lactoferrin and lactobacillus acidophilus composite micro-capsule.
Background technology
Lactoferrin be a kind of iron in conjunction with glycoprotein, be extensively present in the exocrine secretions such as milk, saliva, tear or blood plasma, neutrophil leucocyte.It is a kind of protein with various biological function, can participate in the transhipment of iron, has solubilization to the iron ion in intestines, and has the functions such as broad-spectrum antiseptic, anti-oxidant, anticancer, immunity moderation system.Because lactoferrin has the extensively biological function of uniqueness and physicochemical property, as the emerging member of food, medicine and other fields, receiving increasing concern.It will have broad application prospects in treatment, nutritional supplementation, food etc., therefore have very much R and D and be worth.
Lactobacillus acidophilus does not exist only in stomach, the main probio in it or human small intestine.It can adjust gut flora balance, suppresses the propagation of harmful intestinal tract microorganism.Lactobacillus acidophilus has antagonism to pathogenic microorganisms.The antibiotin class materials such as the acidolin secreted by it, bacillus acidophilus's element, lactein, effectively can resist the growth of pathogenic entero becteria, alleviate liver detoxification burden.After lactobacillus acidophilus is fermented in enteron aisle, also can produce lactic acid and acetic acid, the utilization rate of calcium, phosphorus, iron can be improved, promote the absorption of iron and vitamin D, produce vitamin K and Cobastab, the absorption of cholesterol can also be reduced, and the injury of Radiation On Human body can be reduced.
Recent years, bovine colostrum product has become the focus of food and functional dairy product exploitation, and colostrum manufacturing and processing enterprise, bovine colostrum product kind get more and more.But owing to starting late, the most scale of colostrum manufacturing enterprise of current China is less, product quality is not high, mainly with the colostrum class power-product that common colostrum is produced for raw material, specificity lactoferrin product is little, and these products mainly apply the heat treatment technics such as pasteurize, spraying dry in process of production simultaneously, and in product, the activity of lactoferrin has greater loss, not easily preservation, stomach and intestine percent of pass is very low.This experiment is then explore how under the condition of suitability for industrialized production, keeps the dual biological activity of lactoferrin and lactobacillus acidophilus to greatest extent, overcomes killing and wounding of hydrochloric acid in gastric juice simultaneously, strengthens goods to the health care of human body.
Summary of the invention
The invention provides the preparation method of a kind of high-concentration lactoferrin and lactobacillus acidophilus composite micro-capsule, with the lactobacillus acidophilus of fresh colostrum and High Density Cultivation for raw material, adopt the preparation of ultrafiltration concentration, Vacuum Freezing & Drying Technology simultaneously to possess composite micro-capsule that immunity and Tiny ecosystem regulate double activity.High-concentration lactoferrin and lactobacillus acidophilus is rich in the colostral milk products that the method obtains.
It realizes as follows:
1) the fresh colostrum of centrifugal degreasing under 0-4 DEG C of condition through the centrifugal 20min degreasing of 3000-3500r/min;
2) isoelectric point precipitation is removed casein and skimmed milk is placed in 35-40 DEG C of water-bath, slowly drips lactic acid solution and adjusts its pH value to 4.6-4.8, after leaving standstill 30min, through centrifugal at 20 DEG C, removing casein, collect centrifugal after supernatant obtain colostrum whey;
3) micro-filtration membrane sterilization is by pretreated whey ceramic membrane filter, and fat, albumen and thalline etc. residual in removing whey, obtain the whey liquid of clarification;
4) hollow fiber uf membrane system that above-mentioned permeate employing molecule interception is 10kD is carried out ultra-filtration and separation by ultrafiltration concentration, retains the concentrate being rich in lactoferrin;
5) inoculating lactobacillus acidophilus presses the 3-5% access lactobacillus acidophilus of concentrate volume, and 30-40 DEG C of fermented and cultured 24-36h in constant incubator, makes live lactobacillus acidophilus quantity reach 10 9more than cfu/L, obtains the lactoferrin zymotic fluid being rich in live lactobacillus acidophilus body;
6) adding emulsifying agent homogeneous joins in whey fermentation liquid by after glyceride lactate and granulesten Solution with the 0.1%-0.5% of zymotic fluid quality, homogeneous;
7) emulsion Vacuum Concentration under 45-55 DEG C of condition of step 6 being obtained of Vacuum Concentration, makes concentration reach 18-27%;
8) concentrate step 7 obtained slowly joins in the wall material dissolved, and adjustment solution ph is 6.0-8.0;
9) add freeze drying protectant in gained concentrate, add the glucan of 5-10% mass concentration and the sweet mellow wine of 1-10% mass concentration, control the thickness of feed liquid within the scope of 10-20mm;
10) freeze drying is by above-mentioned concentrate pre-freeze extremely-40 DEG C, water vessel is cooled to-60 DEG C, open vavuum pump, system vacuum is evacuated to 10Pa, opens heating, make material obtain certain latent heat of sublimation, and keeping system vacuum at 20-30Pa until freeze-drying, system vacuum reaches 5Pa, keeps certain hour, puts in storage for subsequent use after making the thorough freeze-drying of material;
Closer, described step 2) in, lactic acid solution concentration is 0.8mol/L, and centrifuge speed is 4000-6000r/min.
Closer, in described step 3), ceramic membrane aperture is 0.4-1.2 μm, and during filtration, operating temperature is 50-55 DEG C, and inlet pressure is 0.5MPa, and outlet pressure is 0.27MPa, and surface velocity is 75L/min.
Closer, described step 4), the technological parameter of ultrafiltration is: operating temperature 35-55 DEG C, operation pressure 0.08-0.12MPa, ultrasiltrated rate 3.5-4.5 L/min.
Closer, in described step 6), emulsifying agent is glyceride lactate: granulesten is the mixture of 1:1.
Closer, in described step 8), wall material is carboxymethyl cellulose: Arabic gum is the mixture of 1:1.
The high-concentration lactoferrin prepared by above method and lactobacillus acidophilus composite micro-capsule, the content of lactoferrin is higher, shows through SDS-PAGE Gel electrophoresis results, and the purity of obtained lactoferrin is approximately 48%.Compared with traditional handicraft, owing to adopting membrane technology to carry out " cold sterilization ", low temperature concentrates, and reduces the decomposition of heat-sensitive ingredients in lactoferrin structure, improves the yield of lactoferrin; The composite micro-capsule that simultaneously the method prepares gained can strengthen the resistance of its environment to external world, reduce the loss of activity of lactoferrin in processing, transportation and in human body hydrochloric acid in gastric juice, significantly improve the physiologically active of lactoferrin and lactobacillus acidophilus arrival enteron aisle, effective maintenance immunity and Tiny ecosystem double activated, strengthen product to the health care of human body, improve the surcharge of product.And every physical and chemical index of these goods and sanitary index all meet national standard, be applicable to industrial actual needs, substantially increase production efficiency.
Detailed description of the invention
Below by embodiment, technical scheme of the present invention is described in further detail.
Embodiment
One, the preparation of lactoferrin concentrate
1) alternative 50kg colostrum is placed in large-scale low-temperature centrifuge, and temperature controls at 4 DEG C, through the centrifugal 20min degreasing of 3500r/min.
2) skimmed milk is placed in 38 DEG C of water-baths, slowly dripping concentration is that the lactic acid solution of 0.8mol/L adjusts its pH value to 4.8, after leaving standstill 30min, through the centrifugal 20min of 5000r/min under 20 DEG C of conditions, collect centrifugal after supernatant obtain colostrum whey.
3) by pretreated whey aperture be the ceramic membrane filter of 0.4 μm, filtration parameter is: operating temperature is 55 DEG C, and inlet pressure is 0.5MPa, and outlet pressure is 0.27MPa, and surface velocity is 75L/min.
4) hollow fiber uf membrane system being 10kD by above-mentioned permeate employing molecule interception carries out ultra-filtration and separation, and the technological parameter of ultrafiltration is: operating temperature 51 DEG C, operation pressure 0.11MPa, ultrasiltrated rate 4.2L/min.
5) access lactobacillus acidophilus mother liquor by concentrate volume 5%, in constant incubator, 30 DEG C of anaerobic fermentations cultivate 24h, and live lactobacillus acidophilus quantity reaches 2.74 × 10 9cfu/L;
6) by glyceride lactate and granulesten be 1:1 compound dissolve after join in whey fermentation liquid with 0.3% of zymotic fluid quality, homogeneous;
7) emulsion step 6 obtained is Vacuum Concentration under 50 DEG C of conditions, and concentration is 21%;
8) concentrate slowly being joined carboxymethyl cellulose, the Arabic gum mixing ratio of having dissolved is in the wall material of 1:1, regulates solution ph to be 6.5 with 2NHCl;
9) in gained concentrate, add the glucan of 5% mass concentration and the sweet mellow wine of 4% mass concentration, the dress liquid thickness of feed liquid is 10mm;
10) by above-mentioned concentrate first pre-freeze 2h under-40 DEG C of conditions, water vessel is cooled to-60 DEG C, open vavuum pump, system vacuum is evacuated to 10Pa, open heating, and keeping system vacuum at 30Pa until freeze-drying, system vacuum reaches 5Pa, keep 30min, put in storage for subsequent use after making the thorough freeze-drying of material;
Test example product of the present invention is to the research of immune function of mice aspect
1. test material and method
1.1 animals used as test and grouping:
SPF level Male Kunming strain mice 120, body weight is 18-22g, is provided by Henan Province's Experimental Animal Center, animal used as test production licence number SCXK(Henan) 2005-0001.Feed is provided by Changsha Kaifu District Dong Chuan Animal Science service department, and production licence number is SCXK(Hunan) 2006-0001.Every 30 are divided into 1 group, totally four groups.Every large group is divided into distilled water control group, common colostrum control group, high-concentration lactoferrin and lactobacillus acidophilus composite micro-capsule group at random, often organizes 10 mouse.
1.2 dosage choice and sample treatment:
Get high-concentration lactoferrin and lactobacillus acidophilus composite micro-capsule 1.67g(converts gained by human oral's recommended amounts) adding distil water is to 200ml, get common colostrum 1.67g adding distil water to 200ml, blank group gives isopyknic distilled water, give animal subject gavage respectively, every day gavage once, gavage volume is 4ml, continuous 30 days.
1.3 experimental techniques:
1.3.1 delayed allergy (DTH) (the sufficient sole of the foot thickens method)
Mouse peritoneal injection 2% (v/v) SRBC(0.2ml/ every mouse) after sensitization 4 days, measure left back sufficient sole of the foot portion thickness, then measuring point hypodermic injection 20% (v/v) SRBC (the every mouse of 20ul/), after injection, 24h measures left back sufficient sole of the foot portion thickness, same position measures three times, averages.To attack the degree that the sufficient sole of the foot thickness difference (swelling degree of the paw) in front and back represents DTH.
1.3.2 antibody-producting cell detects (Jerne improves slide method)
Get sheep blood, with brine 3 times, hematocrit SRBC physiological saline is made into the cell suspension of 2% (v/v) by centrifugal (2000 r/min) l0min. at every turn, every mouse lumbar injection 0.2ml.By the sacrifice of rear for immunity 4 days, get spleen, grind gently, make cell suspension with Hanks liquid, 200 eye mesh screens filter, washing, centrifugal 2 times, finally cell is suspended in 8ml Hanks liquid, counting cells, and cell concentration is adjusted to 5 × 10 6individual/mL.Mix after the culture medium heating for dissolving of top layer with the pH7.4 of equivalent, the Hanks liquid of 2 times of concentration, packing small test tube, often pipe 0.5ml, then in pipe, add 10%SRBC50ul (v/v), the 20ul splenocyte suspension (5 × 10 with the preparation of SA liquid 6individual/mL), be poured on the slide of brush thin layer agarose after rapid mixing, slide level being buckled after agarose solidifies is placed on slide frame, put into CO2gas incubator incubation 1.5h, then join in slide groove with the complement (1:8) of SA liquid dilution, after continuing incubation 1.5h, count hemolysis plaque number.
1.3.3 the mensuration (determination of lactate dehydrogenase method) of NK cytoactive
Test mice cervical dislocation is put to death, asepticly get spleen, make splenocyte suspension, 2 times are washed with Hanks liquid, each centrifugal 10min (l000r/min), abandon supernatant cytoplasm is upspring, add 0.5ml sterilized water 20 seconds, 0.5ml 2 times of Hanks liquid and 8mlHanks liquid is added again after splitting erythrocyte, the centrifugal 10min of 1000rpm, resuspended containing the RPMI1640 complete culture solution of 10% calf serum with 1mL, with counting after 1% glacial acetic acid dilution, with the blue dyeing counting viable count of platform phenol (viable count should more than 95%), adjustment cell concentration be 2 × 10 7individual/ml, this is effector cell, and getting the well-grown YAC-1 cell of 24h after going down to posterity RPMI1640 complete culture solution adjustment cell concentration is 4 × 10 5individual/ml, this is target cell; Get target cell and each 100ul of effector cell (effect target is than 50:1), add in U-shaped 96 well culture plates; Target cell Spontaneous release hole adds target cell and each 100ul of nutrient solution, and the maximum release aperture of target cell adds target cell and each 100ul of 2.5%Triton; Above-mentionedly everyly all establish three parallel holes, in 37 DEG C, 4h is cultivated in 5% CO2gas incubator, then by 96 well culture plates with the centrifugal 5min of 1500r/min, every hole is drawn in 96 well culture plates at the bottom of supernatant 100ul horizontalization, adds LDH matrix liquid 100ul, according to room temperature reaction 3-10min simultaneously, every hole adds the HCl 30ul of 1mol/L, measures optical density (OD) at ELIASA 490nm place.
NK cytoactive=[(reacting hole OD-Spontaneous release hole OD)/(maximum release aperture OD-Spontaneous release hole OD)] × 100%
2. result of the test
2.1 products of the present invention are on the impact of mouse delayed allergy (DTH)
As shown in table 1, mouse more common colostrum control group of its paw swelling of 24h after injection SRBC of test group improves a lot, and compare with blank group and colostrum control group, its P value is all less than 0.05, shows that the delayed allergy ability of mouse after taking product of the present invention is significantly increased.
Table 1 product of the present invention is on the impact (x ± s) of mouse delayed allergy (DTH)
2.2 products of the present invention are on the impact of mouse antibodies cellulation number
As shown in table 2, the more common colostrum control group of mouse hemolysis plaque number of test group improves a lot, and compare with blank group and common colostrum control group, its P value is all less than 0.05, and the antibody-producting cell number showing mouse after taking product of the present invention comparatively control group is significantly increased.
Table 2 product of the present invention is on the impact (x ± s) of mouse antibodies cellulation number
2.3 products of the present invention are on the impact of NK cells in mice activity
As shown in table 3, the more common colostrum control group of NK cells in mice activity of test group improves a lot, and compare with blank group and common colostrum control group, its P value is all less than 0.05, and the NK cytoactive showing mouse after taking product of the present invention comparatively control group is significantly increased.
Table 3 product of the present invention is on the impact (x ± s) of NK cells in mice activity
Should be understood that above-described embodiment is only that protection scope of the present invention is not limited thereto in order to absolutely prove the preferred embodiment that the present invention lifts.The equivalent alternative or conversion that those skilled in the art do on basis of the present invention, all within protection scope of the present invention.Protection scope of the present invention is as the criterion with claims.

Claims (5)

1. a preparation method for high-concentration lactoferrin and lactobacillus acidophilus composite micro-capsule, is characterized in that comprising the steps:
1) centrifugal degreasing: fresh colostrum under 0-4 DEG C of condition through the centrifugal 20min degreasing of 3000-3500r/min;
2) isoelectric point precipitation removes casein: skimmed milk is placed in 35-40 DEG C of water-bath, slowly drips lactic acid solution and adjusts its pH value to 4.6-4.8, after leaving standstill 30min, centrifugal removing casein under 20 DEG C of conditions, collect centrifugal after supernatant obtain colostrum whey; Lactic acid solution concentration is 0.8mol/L, and centrifuge speed is 4000-6000r/min;
3) micro-filtration membrane sterilization: by above-mentioned colostrum whey ceramic membrane filter, fat, albumen and thalline etc. residual in removing whey, obtain the whey liquid of clarification;
4) ultrafiltration concentration: the hollow fiber uf membrane system being 10kD by above-mentioned permeate employing molecule interception carries out ultra-filtration and separation, retains the concentrate being rich in lactoferrin;
5) inoculating lactobacillus acidophilus: access high density lactobacillus acidophilus by the 3-5% of concentrate volume, 30-40 DEG C of fermented and cultured 24-36h in constant incubator, makes live lactobacillus acidophilus quantity reach 10 9more than cfu/L, obtains the lactoferrin zymotic fluid being rich in live lactobacillus acidophilus body;
6) emulsifying agent homogeneous is added: join in lactoferrin zymotic fluid by after glyceride lactate and soybean lecithin Solution with the 0.1%-0.5% of zymotic fluid quality, homogeneous;
7) Vacuum Concentration: emulsion step 6 obtained is Vacuum Concentration under 45-55 DEG C of condition, makes concentration reach 18-27%;
8) concentrate step 7 obtained slowly joins in the wall material dissolved, and adjustment solution ph is 6.0-8.0;
9) add freeze drying protectant: in gained concentrate, add the glucan of 5-10% mass concentration and the sweet mellow wine of 1-10% mass concentration, control the thickness of feed liquid within the scope of 10-20mm;
10) freeze drying: by the concentrate pre-freeze of step 9 gained to-40 DEG C, water vessel is cooled to-60 DEG C, open vavuum pump, system vacuum is evacuated to 10Pa, opens heating, make material obtain certain latent heat of sublimation, and keeping system vacuum at 20-30Pa until freeze-drying, system vacuum reaches 5Pa, keeps certain hour, puts in storage for subsequent use after making the thorough freeze-drying of material.
2. the preparation method of high-concentration lactoferrin as claimed in claim 1 and lactobacillus acidophilus composite micro-capsule, it is characterized in that: in described step 3), ceramic membrane aperture is 0.4-1.2 μm, during filtration, operating temperature is 50-55 DEG C, inlet pressure is 0.5MPa, outlet pressure is 0.27MPa, and surface velocity is 75L/min.
3. the preparation method of high-concentration lactoferrin as claimed in claim 1 and lactobacillus acidophilus composite micro-capsule, it is characterized in that: in described step 4), the technological parameter of ultrafiltration is: operating temperature 35-55 DEG C, operation pressure 0.08-0.12MPa, ultrasiltrated rate 3.5-4.5 L/min.
4. the preparation method of high-concentration lactoferrin as claimed in claim 1 and lactobacillus acidophilus composite micro-capsule, it is characterized in that: in described step 6), emulsifying agent is glyceride lactate: soybean lecithin is the mixture of 1:1.
5. the preparation method of high-concentration lactoferrin as claimed in claim 1 and lactobacillus acidophilus composite micro-capsule, is characterized in that: described step 8) mesospore material is carboxymethyl cellulose: Arabic gum is the mixture of 1:1.
CN201310693004.8A 2013-12-18 2013-12-18 Method for preparing compound microcapsule of high-concentration lactoferrin and lactobacillus acidophilus Active CN103734745B (en)

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CN105558041A (en) * 2015-12-14 2016-05-11 东北农业大学 Preparation method of food-grade lactoferrin micro-emulsion with heat-resisting protection effect
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