CN103734741B - A kind of preparation method and application of wolfberry fruit extract - Google Patents

A kind of preparation method and application of wolfberry fruit extract Download PDF

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CN103734741B
CN103734741B CN201310742729.1A CN201310742729A CN103734741B CN 103734741 B CN103734741 B CN 103734741B CN 201310742729 A CN201310742729 A CN 201310742729A CN 103734741 B CN103734741 B CN 103734741B
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extract
filtrate
filter
filter residue
wolfberry fruit
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CN103734741A (en
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张志年
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Jiangsu Haofeng Biotechnology Co ltd
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Xuzhou Lvzhiye Biological Foodstuff Co Ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/51—Concentration
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52—Adding ingredients
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
    • A23P10/00—Shaping or working of foodstuffs characterised by the products
    • A23P10/40—Shaping or working of foodstuffs characterised by the products free-flowing powder or instant powder, i.e. powder which is reconstituted rapidly when liquid is added
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/50—Soya sauce
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/60—Salad dressings; Mayonnaise; Ketchup
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
    • A23P10/00—Shaping or working of foodstuffs characterised by the products
    • A23P10/20—Agglomerating; Granulating; Tabletting
    • A23P10/28—Tabletting; Making food bars by compression of a dry powdered mixture
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
    • A23P10/00—Shaping or working of foodstuffs characterised by the products
    • A23P10/30—Encapsulation of particles, e.g. foodstuff additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C5/00—Other raw materials for the preparation of beer
    • C12C5/02—Additives for beer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
    • C12G3/00—Preparation of other alcoholic beverages
    • C12G3/04—Preparation of other alcoholic beverages by mixing, e.g. for preparation of liqueurs

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Non-Alcoholic Beverages (AREA)
  • Preparation Of Fruits And Vegetables (AREA)

Abstract

The invention discloses a kind of preparation method and application of wolfberry fruit extract.The method is mixed with benzinum by matrimony vine respectively, through extraction, Separation of Solid and Liquid, filtrate is through distilling to obtain extract, filter residue carries secondary through adding hydro-thermal again, filter, concentrating filter liquor obtains extract, extract is mixed, dry, be ground into the obtained and matrimony vine of 500-700 order fine powder and mix with ethanol, through extraction, Separation of Solid and Liquid, filtrate is through distilling to obtain extract, and filter residue carries secondary through adding hydro-thermal again, filters, concentrating filter liquor obtains extract, extract is mixed, dry, be ground into 500-700 order fine powder and obtain extract product.The inventive method is extracted completely, and efficiency is high, and economic worth is large, can be used as in wine goods, soft, solid beverage, flavor enhancement and nutriment and applies.

Description

A kind of preparation method and application of wolfberry fruit extract
Technical field
The present invention relates to the preparation method of the fruit extract of natural plants, also relate to field of functional food, particularly a kind of preparation method and application of wolfberry fruit extract.
Background technology
Matrimony vine taste is sweet and refreshing, nutritious, medicinal and edible with a long history to matrimony vine of China, dry fruit, herbal cuisine and dish is mainly contained as nutritious tonifying is edible, be developed to the product supply markets such as beverage, drinks, drying dried nuts, wolfberry fruit powder, medlar preserved fruit in recent years, meet the portion requirements in market to some extent, but, also be far from as a kind of matrimony vine with very high nutritive value and health-care efficacy being dissolved in the life of ordinary citizen and go, matrimony vine can't be widely used and more people is benefited.Along with the raising of people's living standard, pursue healthy, the hope of improving the quality of living makes health products trade constantly bring forth new ideas, and manufactures the more useful product with nutritve function to meet the demand of people.Matrimony vine, as a kind of tonic Chinese herbal medicine having long history in China, is constantly developed to different useful dosage form product in recent years, in different modes of nourishing, meets market segment demand.Along with the progress of science and technology, scientific research personnel starts with from the beneficiating ingredient matrimony vine, extracts respectively, makes matrimony vine be not only a kind of organically mixture, but ask its special efficacy from some distinctive composition Zhongmou County, as LBP-X is extensively applied.
Because matrimony vine contains diversity and the complexity of composition, a lot of single component is also difficult to effectively be extracted or utilize.Effective raw material application using wolfberry fruit extract as some goods in prior art is still general, and in order to make the active ingredient of matrimony vine be fully used, it is that a kind of technique is simple, production cost is low that its extract does not still lose, the reliable effective ways of effect.
In existing disclosed technology, as Chinese patent CN102924617A discloses " wolfberry fruit extract and preparation method thereof ", the method for this invention take Chinese wolfberry fruit as raw material, add water, then matrimony vine and aqueous mixtures are pulverized, obtain solid mixture slurries, slurries ultrafilter is concentrated, obtains concentrate, in concentrate, add the ethanol of volumetric concentration 40-60%, leave standstill, filter, add the ethanol of volumetric concentration 70-80% in filtrate, staticly settle thing, drying, obtains dry thing.By dry thing, then use water-soluble solution, be greater than the solution of 10000 by ultrafiltration molecular cut off, concentrated, concentrate is adsorbed in non-polar macroporous resin, is the toluene wash-out of 60-70% by concentration, reclaims toluene, obtains wolfberry fruit extract after dry, concentrated.Therefrom can find out that this invention is adsorbed through non-polar macroporous resin with water extract-alcohol precipitation again, then obtain product with toluene wash-out.Chinese patent CN102988605A adopts " supercritical CO 2the method of extraction wolfberry fruit extract ", mainly LBP-X in the lycium plant extract of the method for this technology extraction, the content of its LBP-X reaches 17%.Chinese patent CN103044569A discloses " wolfberry fruit extract containing matrimony vine acid and preparation method thereof ", this technical scheme shows matrimony vine acid containing 1%-10% in described wolfberry fruit extract and 2%-20% LBP-X, its preparation method comprise matrimony vine raw material reverse osmosis water washed away floating dust, reverse osmosis water lixiviate or heating extraction, filtration, the absorption of ion-exchange fibre post, wash-out, ultrafiltration, concentrate, sterilizing, spraying dry obtain product.What select during Chinese patent CN1101240A discloses " bean instant drink and production method thereof " is jujube medlar medicinal extract, the preparation of this medicinal extract is the date 4kg, the matrimony vine 1kg that take free from insect pests, about 3 times amount that add water are heated to 90 DEG C-100 DEG C, extract 2 hours, filter, filter filter residue, then extracting in water 1 hour, and three filtrates.Be condensed into the mixing medicinal extract (the 5th page of the 3rd section of 1-5 is capable for description) of same inventory weight ratio 1:1.Chinese patent CN102240035A discloses " a kind of preparation method of wolfberry extract health-care granules ", its technical scheme carries out supercritical carbon dioxide extracting by after matrimony vine ultramicro grinding, obtain liquid extract and solid-state extract remainder, then water-soluble for solid-state extract remainder extraction is obtained water-soluble extract, above-mentioned gained liquid extract and the water-soluble extract after concentrating are mixed, add sucrose, dextrin, drying and crushing, is wolfberry extract health-care granules after adopting dry method granulation processes to granulate after sieving.Chinese patent CN101991709A discloses " method for preparing antioxidant lycium ruthenicum extract ", this invention lixiviate, and high-pressure homogeneous, UF membrane and macroreticular resin are separated, dry, obtains anti-oxidant black fruit lyceum extract.
From above-mentioned existing published technical scheme, can find out that obtained wolfberry fruit extract mostly adopts water extraction, concentrates; Water extraction, separation, resin adsorption, concentrated; Water extraction, alcohol precipitation, the technique such as concentrated, the composition of its wolfberry fruit extract has single composition targetedly mostly.But, except containing except LBP-X in matrimony vine, still containing betaine, carotenoid, carotenoids ester, vitamin C, scopoletin, taurine, reduced sugar, fructose, sucrose, aldehyde radical sugar, cyclic peptide, matrimony vine element A-D, several amino acids and various trace elements; Wherein amino acid is containing 19 seed amino acids (comprising 8 kinds of essential amino acids), and total amount reaches 9.14%, and wherein element class contains mineral element and the trace elements such as manganese, zinc, copper, cobalt, cadmium, nickel, sodium, calcium, magnesium, potassium, strontium, selenium.Water soluble ingredient is had in composition described above, also liposoluble constituent is had, the preparation method of existing wolfberry fruit extract, the active ingredient in its matrimony vine is difficult to be extracted more fully, its effect also will disappear because of the loss of its composition, makes wolfberry fruit extract more effectively can not play its maximum effect.
Summary of the invention
The object of the invention is to for the deficiencies in the prior art, provide a kind of active ingredient by matrimony vine fully to be extracted, the wolfberry fruit extract obtained, is more conducive to the beneficial effect of absorption of human body and improving product effect.
The object of this invention is to provide a kind of preparation method of wolfberry fruit extract.
The present invention also aims to provide a kind of wolfberry fruit extract as the application in wine goods, flavouring, beverage and nutriment.
Except as otherwise noted, percentage of the present invention is percetage by weight.
Object of the present invention is achieved by the following technical programs:
A preparation method for wolfberry fruit extract, the method comprises the step of following order:
(1) get matrimony vine pure water clean up and drain away the water, wear into fine and smooth homogenate with colloid mill;
(2) mixed with benzinum by wolfberry fruit syrup, mixed weight is than being 1:15-30;
(3) 20-40h is extracted at ambient temperature;
(4) Separation of Solid and Liquid, collects supernatant liquid and filter residue respectively;
(5) liquid decompression distillation 0.5-2.5h under vacuum 0.01-0.1Mpa, vapo(u)rizing temperature 25 DEG C of-30 DEG C of conditions, for subsequent use except namely obtaining extract after desolventizing;
(6) added water by step (4) filter residue, heat carries, adjust pH value, repeat hot water stirs and extract 2 times, filter, merging filtrate, centrifugal removal of impurities, essence filter obtains filtrate;
(7) filtrate is concentrated into heavy paste extract through thin film evaporation for subsequent use;
(8) step (5) is mixed with step (7) is extract obtained, vacuum drying or freeze drying, be ground into fine powder and cross 500-700 mesh sieve, pack and obtain wolfberry fruit extract.
The preparation method of a kind of wolfberry fruit extract described above, wherein said filter residue adds water, heat is carried, and adjusts pH value, and hot water stirs extracts, and filters, centrifugal removal of impurities, and essence filter step is 1:5-10 by matrimony vine solid-liquid ratio; Hot temperature raising degree is 45-65 DEG C; Adjustment material liquid PH value is 4.6-5.5; Speed of agitator is 35-65rpm; Extraction time is 80-160min; Filter with 200 order industrial filter cloths; Centrifugal removal of impurities condition is 12000-16000r/min, and the time is 15min; Essence filter employing 0.22 μm of miillpore filter.
Described matrimony vine is well-done fresh fructus lycii or commercially available ripe dried fruit of lycium barbarum.
The preferred medical grade benzinum of described benzinum.
When described medical grade benzinum boiling point is 60 DEG C-90 DEG C, the preferred 20h of extraction time.
When described medical grade benzinum boiling point is 30 DEG C-60 DEG C, the preferred 40h of extraction time.
A kind of wolfberry fruit extract is obtained by preparation method described above.
Present invention also offers a kind of preparation method of wolfberry fruit extract, it is characterized in that the method comprises the step of following order:
(1) get matrimony vine peace and quiet with pure water, drop is moisture to the greatest extent, wears into fine and smooth homogenate with colloid mill;
(2) ethanol being 50-100% by wolfberry fruit syrup and concentration mixes, and mixed weight is than being 1:5-15;
(3) under 25 DEG C of-45 DEG C of conditions, 1-240h is extracted;
(4) Separation of Solid and Liquid, collects supernatant liquid and filter residue respectively;
(5) liquid first air-distillation 0.5h at 85 DEG C, then vacuum be 0.01-0.1Mpa, vapo(u)rizing temperature be 25 DEG C of-65 DEG C of conditions under decompression distillation 1.0-2.5h, obtain extract for subsequent use;
(6) step (4) filter residue is added hydro-thermal to carry, adjust pH value, repeat hot water stirs and extract 2 times, filter, merging filtrate, centrifugal removal of impurities, essence filters to obtain filtrate;
(7) filtrate is concentrated into heavy paste extract through thin film evaporation for subsequent use;
(8) step (5) extract is mixed with step (7) extract, vacuum drying, be ground into fine powder and cross 500-700 mesh sieve, pack and obtain wolfberry fruit extract.
The preparation method of a kind of wolfberry fruit extract described above, wherein said filter residue adds water, heat is carried, and adjusts pH value, and hot water stirs extracts, and filters, centrifugal removal of impurities, and essence filter step is 1:10-15 by matrimony vine solid-liquid ratio; Water extraction temperature is 45-65 DEG C; Adjustment material liquid PH value is 4.6-5.5; Speed of agitator is 35-65rpm; Extraction time is 80-160min; Filter with 200 order industrial filter cloths; Centrifugal removal of impurities condition is 12000-16000r/min, and the time is 15min; Essence filter employing 0.22 μm of miillpore filter.
Wherein, described matrimony vine is well-done fresh fructus lycii or commercially available ripe dried fruit of lycium barbarum.
Present invention also offers a kind of wolfberry fruit extract as the application in wine goods, soft drink, particle beverage; Seasoning is as baste, soy sauce, vinegar, tartar sauce, soup stock, and nutriment is as the application in the formulations such as tablet, soft capsule, hard shell capsules, granule, oral liquid.
Beneficial effect of the present invention
1, the present invention be by matrimony vine respectively after benzinum and alcohol extract, its filter residue is again through water extraction, obtained extract respectively, again extract is mixed, vacuum drying, be ground into 500-700 order fine powder, through packing the two kinds of preparation methods made, its advantage is after the liposoluble constituent in matrimony vine is fully extracted, again its water soluble ingredient is carried out water extraction, obtain extract, make the beneficiating ingredient of matrimony vine can obtain extracting completely fully, make the beneficiating ingredient of extract not reason unilaterally extract and run off, make extract really play the effect of its beneficiating ingredient.
2, the inventive method technique is simple, and production cost is low, and extraction efficiency is high, and economic worth is large, and suitability for industrialized is produced, and production process can effectively recycle by recycling design, non-environmental-pollution.
3, the present invention adopts organic solvent and water to be that solvent extracts wolfberry fruit extract, and expanded the new method developed matrimony vine, the extract of extraction is after micro mist process, its Dispersion on surface degree increases, and makes consumption little, because product is fine and smooth, granularity is little, is beneficial to absorption and the utilization of human body.
Detailed description of the invention
Embodiment 1
(1) take dried fruit of lycium barbarum 10 kilograms, after cleaning up with pure water, drain away the water, wear into fine and smooth homogenate with colloid mill; (2) load extractor, add the medical grade benzinum that 150 liters of boiling points are 60 DEG C-90 DEG C; (3) under 22 DEG C of conditions, 20h is soaked; (4) with flame filter press filter, respectively clarified solution and filter residue for subsequent use; (5) clarified solution (4) obtained is decompression distillation 1h under vacuum 0.06MPa, vapo(u)rizing temperature 30 DEG C of conditions, obtains extract except after desolventizing; (6) filter residue in (4) is added pure water 100 kilograms, be heated to 50 DEG C, adjustment pH value is 4.8, being set in mixer rotating speed is 40rpm, after extracting 160min, filters with 200 order industrial filter cloths under constantly stirring, respectively filtrate and filter residue for subsequent use, filter residue similarity condition repeats extraction 1 time, filters with 200 order industrial filter cloths, merges secondary filtrate, with centrifuge centrifugal 15min under the condition of 12000r/min, obtain centrifugate, then use 0.22 μm of filtering with microporous membrane, obtain filtrate; (7) filtrate is concentrated into heavy paste extract through thin film evaporation; (8) mix extract obtained to (5) with (7) are extract obtained, through vacuum drying, be ground into 500-700 order fine powder with airslide disintegrating mill; Adopt aluminum foil compound bag vacuum packaging, obtain wolfberry fruit extract product.
Embodiment 2
(1) take well-done new fresh fructus lycii 10 kilograms, after cleaning up with pure water, drain away the water, wear into fine and smooth homogenate with colloid mill; (2) load extractor, add the medical grade benzinum that 200 liters of boiling points are 60 DEG C-90 DEG C; (3) under 25 DEG C of conditions, 30h is soaked; (4) with flame filter press filter, respectively clarified solution and filter residue for subsequent use; (5) clarified solution (4) obtained is decompression distillation 2h under vacuum 0.08MPa, vapo(u)rizing temperature 28 DEG C of conditions, obtains extract except after desolventizing; (6) filter residue in (4) is added pure water 50 kilograms, be heated to 65 DEG C, adjustment pH value is 5.0, being set in mixer rotating speed is 65rpm, after extracting 90min, filters with 200 order industrial filter cloths under constantly stirring, respectively filtrate and filter residue for subsequent use, filter residue similarity condition repeats extraction 1 time, filters with 200 order industrial filter cloths, merges secondary filtrate, with centrifuge centrifugal 15min under the condition of 15000r/min, obtain centrifugate, then use 0.22 μm of filtering with microporous membrane, obtain filtrate; (7) filtrate is concentrated into heavy paste extract through thin film evaporation; (8) mix extract obtained to (5) with (7) are extract obtained, through vacuum drying, be ground into 500-700 order fine powder with airslide disintegrating mill; Adopt aluminum foil compound bag vacuum packaging, obtain wolfberry fruit extract product.
Embodiment 3
(1) take well-done new fresh fructus lycii 10 kilograms, after cleaning up with pure water, drain away the water, wear into fine and smooth homogenate with colloid mill; (2) load extractor, add the medical grade benzinum that 300 liters of boiling points are 60 DEG C-90 DEG C; (3) under 23 DEG C of conditions, 25h is soaked; (4) with flame filter press filter, respectively clarified solution and filter residue for subsequent use; (5) clarified solution (4) obtained is decompression distillation 2.5h under vacuum 0.1MPa, vapo(u)rizing temperature 30 DEG C of conditions, obtains extract except after desolventizing; (6) filter residue in (4) is added pure water 75 kilograms, be heated to 60 DEG C, adjustment pH value is 5.5, being set in mixer rotating speed is 60rpm, after extracting 120min, filters with 200 order industrial filter cloths under constantly stirring, respectively filtrate and filter residue for subsequent use, filter residue similarity condition repeats extraction 1 time, filters with 200 order industrial filter cloths, merges secondary filtrate, with centrifuge centrifugal 15min under the condition of 16000r/min, obtain centrifugate, then use 0.22 μm of filtering with microporous membrane, obtain filtrate; (7) filtrate is concentrated into heavy paste extract through thin film evaporation; (8) mix extract obtained to (5) with (7) are extract obtained, through vacuum drying, be ground into 500-700 order fine powder with airslide disintegrating mill; Adopt aluminum foil compound bag vacuum packaging, obtain wolfberry fruit extract product.
Embodiment 4
(1) take dried fruit of lycium barbarum 10 kilograms, after cleaning up with pure water, drain away the water, wear into fine and smooth homogenate with colloid mill; (2) load extractor, add the medical grade benzinum that 300 liters of boiling points are 30 DEG C-60 DEG C; (3) under 25 DEG C of conditions, 40h is soaked; (4) with flame filter press filter, respectively clarified solution and filter residue for subsequent use; (5) clarified solution (4) obtained is decompression distillation 1.5h under vacuum 0.03MPa, vapo(u)rizing temperature 25 DEG C of conditions, obtains extract except after desolventizing; (6) filter residue in (4) is added pure water 90 kilograms, be heated to 65 DEG C, adjustment pH value is 4.6, being set in mixer rotating speed is 35rpm, after extracting 150min, filters with 200 order industrial filter cloths under constantly stirring, respectively filtrate and filter residue for subsequent use, filter residue similarity condition repeats extraction 1 time, filters with 200 order industrial filter cloths, merges secondary filtrate, with centrifuge centrifugal 15min under the condition of 13000r/min, obtain centrifugate, then use 0.22 μm of filtering with microporous membrane, obtain filtrate; (7) filtrate is concentrated into heavy paste extract through thin film evaporation; (8) mix extract obtained to (5) with (7) are extract obtained, through vacuum drying, be ground into 500-700 order fine powder with airslide disintegrating mill; Adopt aluminum foil compound bag vacuum packaging, obtain wolfberry fruit extract product.
Embodiment 5
(1) take well-done new fresh fructus lycii 10 kilograms, after cleaning up with pure water, drain away the water, wear into fine and smooth homogenate with colloid mill; (2) load extractor, add the medical grade benzinum that 150 liters of boiling points are 30 DEG C-60 DEG C; (3) under 23 DEG C of conditions, 38h is soaked; (4) with flame filter press filter, respectively clarified solution and filter residue for subsequent use; (5) clarified solution (4) obtained is decompression distillation 2h under vacuum 0.05MPa, vapo(u)rizing temperature 26 DEG C of conditions, obtains extract except after desolventizing; (6) filter residue in (4) is added pure water 100 kilograms, be heated to 60 DEG C, adjustment pH value is 5.2, being set in mixer rotating speed is 40rpm, after extracting 120min, filters with 200 order industrial filter cloths under constantly stirring, respectively filtrate and filter residue for subsequent use, filter residue similarity condition repeats extraction 1 time, filters with 200 order industrial filter cloths, merges secondary filtrate, with centrifuge centrifugal 15min under the condition of 15000r/min, obtain centrifugate, then use 0.22 μm of filtering with microporous membrane, obtain filtrate; (7) filtrate is concentrated into heavy paste extract through thin film evaporation; (8) mix extract obtained to (5) with (7) are extract obtained, through vacuum drying, be ground into 500-700 order fine powder with airslide disintegrating mill; Adopt aluminum foil compound bag vacuum packaging, obtain wolfberry fruit extract product.
Embodiment 6
(1) take well-done new fresh fructus lycii 10 kilograms, after cleaning up with pure water, drain away the water, wear into fine and smooth homogenate with colloid mill; (2) load extractor, add the medical grade benzinum that 200 liters of boiling points are 30 DEG C-60 DEG C; (3) under 22 DEG C of conditions, 30h is soaked; (4) with flame filter press filter, respectively clarified solution and filter residue for subsequent use; (5) clarified solution (4) obtained is decompression distillation 2.5h under vacuum 0.06MPa, vapo(u)rizing temperature 28 DEG C of conditions, obtains extract except after desolventizing; (6) filter residue in (4) is added pure water 70 kilograms, be heated to 45 DEG C, adjustment pH value is 5.0, being set in mixer rotating speed is 65rpm, after extracting 80min, filters with 200 order industrial filter cloths under constantly stirring, respectively filtrate and filter residue for subsequent use, filter residue similarity condition repeats extraction 1 time, filters with 200 order industrial filter cloths, merges secondary filtrate, with centrifuge centrifugal 15min under the condition of 12000r/min, obtain centrifugate, then use 0.22 μm of filtering with microporous membrane, obtain filtrate; (7) filtrate is concentrated into heavy paste extract through thin film evaporation; (8) mix extract obtained to (5) with (7) are extract obtained, through vacuum drying, be ground into 500-700 order fine powder with airslide disintegrating mill; Adopt aluminum foil compound bag vacuum packaging, obtain wolfberry fruit extract product.
Embodiment 7
(1) take dried fruit of lycium barbarum 10 kilograms, after cleaning up with pure water, drain away the water, wear into fine and smooth homogenate with colloid mill; (2) load extractor, add the ethanol 150 kilograms that concentration is 65%; (3) under 25 DEG C of conditions, 240h is extracted; (4) with flame filter press filter, respectively clarified solution and filter residue for subsequent use; (5) clarified solution that (4) obtained is air-distillation 0.5h at 85 DEG C first, then vacuum be 0.03MPa, vapo(u)rizing temperature be 30 DEG C of conditions under decompression distillation 2h, obtain extract; (6) filter residue in (4) is added pure water 150 kilograms, be heated to 65 DEG C, adjustment pH value is 4.6, being set in mixer rotating speed is 35rpm, after extracting 160min, filters with 200 order industrial filter cloths under constantly stirring, respectively filtrate and filter residue for subsequent use, filter residue similarity condition repeats extraction 1 time, filters with 200 order industrial filter cloths, merges secondary filtrate, with centrifuge centrifugal 15min under the condition of 12000r/min, obtain centrifugate, then use 0.22 μm of filtering with microporous membrane, obtain filtrate; (7) filtrate is concentrated into heavy paste extract through thin film evaporation; (8) mix extract obtained to (5) with (7) are extract obtained, through vacuum drying, be ground into 500-700 order fine powder with airslide disintegrating mill; Adopt aluminum foil compound bag vacuum packaging, obtain wolfberry fruit extract product.
Embodiment 8
(1) take well-done new fresh fructus lycii 10 kilograms, after cleaning up with pure water, drain away the water, wear into fine and smooth homogenate with colloid mill; (2) load extractor, add the ethanol 50 kilograms that concentration is 95%; (3) under 30 DEG C of conditions, 100h is extracted; (4) with flame filter press filter, respectively clarified solution and filter residue for subsequent use; (5) clarified solution that (4) obtained is air-distillation 0.5h at 85 DEG C first, then vacuum be 0.08MPa, vapo(u)rizing temperature be 60 DEG C of conditions under decompression distillation 1.5h, obtain extract; (6) filter residue in (4) is added pure water 100 kilograms, be heated to 50 DEG C, adjustment pH value is 5.0, being set in mixer rotating speed is 65rpm, after extracting 100min, filters with 200 order industrial filter cloths under constantly stirring, respectively filtrate and filter residue for subsequent use, filter residue similarity condition repeats extraction 1 time, filters with 200 order industrial filter cloths, merges secondary filtrate, with centrifuge centrifugal 15min under the condition of 15000r/min, obtain centrifugate, then use 0.22 μm of filtering with microporous membrane, obtain filtrate; (7) filtrate is concentrated into heavy paste extract through thin film evaporation; (8) mix extract obtained to (5) with (7) are extract obtained, through vacuum drying, be ground into 500-700 order fine powder with airslide disintegrating mill; Adopt aluminum foil compound bag vacuum packaging, obtain wolfberry fruit extract product.
Embodiment 9
(1) take well-done new fresh fructus lycii 10 kilograms, after cleaning up with pure water, drain away the water, wear into fine and smooth homogenate with colloid mill; (2) load extractor, add the ethanol 100 kilograms that concentration is 55%; (3) under 45 DEG C of conditions, 10h is extracted; (4) with flame filter press filter, respectively clarified solution and filter residue for subsequent use; (5) clarified solution that (4) obtained is air-distillation 0.5h at 85 DEG C first, then vacuum be 0.1MPa, vapo(u)rizing temperature be 65 DEG C of conditions under decompression distillation 1h, obtain extract; (6) filter residue in (4) is added pure water 120 kg, be heated to 65 DEG C, adjustment pH value is 4.8, being set in mixer rotating speed is 60rpm, after extracting 80min, filters with 200 order industrial filter cloths under constantly stirring, respectively filtrate and filter residue for subsequent use, filter residue similarity condition repeats extraction 1 time, filters with 200 order industrial filter cloths, merges secondary filtrate, with centrifuge centrifugal 15min under the condition of 16000r/min, obtain centrifugate, then use 0.22 μm of filtering with microporous membrane, obtain filtrate; (7) filtrate is concentrated into heavy paste extract through thin film evaporation; (8) mix extract obtained to (5) with (7) are extract obtained, through vacuum drying, be ground into 500-700 order fine powder with airslide disintegrating mill; Adopt aluminum foil compound bag vacuum packaging, obtain wolfberry fruit extract product.
Application Example 1: the preparation of Lycium chinense wine
After the wolfberry fruit extract that Example 1 is obtained and white wine are in harmonious proportion stir in 1:100-5:80 ratio, under 10 DEG C of environment, ageing is after 70 days, after filtration, fillingly obtains Lycium chinense wine.
Application Example 2: the preparation of matrimony vine beer
After the obtained wolfberry fruit extract of Example 2 is in harmonious proportion in 1:500-3:150 ratio with draft beer, under 5-8 DEG C of environment, ageing is after 30 days, after filtration, sterilization, filling must matrimony vine beer.
Application Example 3: the preparation of Chinese wolfberry fruit drink
With following raw materials according, the preparation method according to general soft drink produces.
The wolfberry fruit extract that Example 3 obtains is base stock
Wolfberry fruit extract 50g
Honey 100g
Citric acid 2g
Tea Polyphenols 1g
Matrimony vine essence 0.5g
Pure water 846.5g
Application Example 4: the preparation of wolfberry fruit sauce
Traditional production of condiments method is adopted to produce
Flour paste 50%, white granulated sugar 5%, citric acid 0.5%, wolfberry fruit extract 10%, vitamin C 0.25%, strawberry jam 34.25%.
Application Example 5: the preparation of medlar soy
The wolfberry fruit extract 100g that Example 5 the is obtained and 900g that makes soy sauce is in harmonious proportion, and is placed in 10-15 DEG C of environment ageing 20 days, after filtration, filling, sterilizing, obtained medlar soy.
Application Example 6: the preparation of matrimony vine soft capsule
Produce with the conventional production process of following raw materials according by general soft capsule.
Wolfberry fruit extract 100mg, vitamin E oil 50mg, palm oil 210mg.
Application Example 7: the preparation of matrimony vine tablet
Produce with the production method of following raw materials according by general tablet.
Wolfberry fruit extract 0.15g, powdered sugar 0.3g, citric acid 0.002g, maltodextrin 0.05g, Magnesium Stearate proper quantity.
Application Example 8: the preparation of capsule
Produce with the production method of following raw materials according by general capsule.
Wolfberry fruit extract 0.25g, cornstarch 0.2g, sodium carboxymethylcellulose 0.05g.

Claims (3)

1. a wolfberry fruit extract, is characterized in that: (1) takes dried fruit of lycium barbarum 10 kilograms, after cleaning up, drains away the water with pure water, wears into fine and smooth homogenate with colloid mill; (2) load extractor, add the ethanol 150 kilograms that concentration is 65%; (3) under 25 DEG C of conditions, 240h is extracted; (4) with flame filter press filter, respectively clarified solution and filter residue for subsequent use; (5) clarified solution that (4) obtained is air-distillation 0.5h at 85 DEG C first, then vacuum be 0.03MPa, vapo(u)rizing temperature be 30 DEG C of conditions under decompression distillation 2h, obtain extract; (6) filter residue in (4) is added pure water 150 kilograms, be heated to 65 DEG C, adjusted to ph is 4.6, being set in mixer rotating speed is 35rpm, after extracting 160min, filters with 200 order industrial filter cloths under constantly stirring, respectively filtrate and filter residue for subsequent use, filter residue similarity condition repeats extraction 1 time, filters with 200 order industrial filter cloths, merges secondary filtrate, with centrifuge centrifugal 15min under the condition of 12000r/min, obtain centrifugate, then use 0.22 μm of filtering with microporous membrane, obtain filtrate; (7) filtrate is concentrated into heavy paste extract through thin film evaporation; (8) mix extract obtained to (5) with (7) are extract obtained, through vacuum drying, be ground into 500-700 order fine powder with airslide disintegrating mill; Adopt aluminum foil compound bag vacuum packaging, obtain wolfberry fruit extract product.
2. a wolfberry fruit extract, is characterized in that: (1) takes well-done new fresh fructus lycii 10 kilograms, after cleaning up, drains away the water with pure water, wears into fine and smooth homogenate with colloid mill; (2) load extractor, add the ethanol 50 kilograms that concentration is 95%; (3) under 30 DEG C of conditions, 100h is extracted; (4) with flame filter press filter, respectively clarified solution and filter residue for subsequent use; (5) clarified solution that (4) obtained is air-distillation 0.5h at 85 DEG C first, then vacuum be 0.08MPa, vapo(u)rizing temperature be 60 DEG C of conditions under decompression distillation 1.5h, obtain extract; (6) filter residue in (4) is added pure water 100 kilograms, be heated to 50 DEG C, adjusted to ph is 5.0, being set in mixer rotating speed is 65rpm, after extracting 100min, filters with 200 order industrial filter cloths under constantly stirring, respectively filtrate and filter residue for subsequent use, filter residue similarity condition repeats extraction 1 time, filters with 200 order industrial filter cloths, merges secondary filtrate, with centrifuge centrifugal 15min under the condition of 15000r/min, obtain centrifugate, then use 0.22 μm of filtering with microporous membrane, obtain filtrate; (7) filtrate is concentrated into heavy paste extract through thin film evaporation; (8) mix extract obtained to (5) with (7) are extract obtained, through vacuum drying, be ground into 500-700 order fine powder with airslide disintegrating mill; Adopt aluminum foil compound bag vacuum packaging, obtain wolfberry fruit extract product.
3. a wolfberry fruit extract, is characterized in that: (1) takes well-done new fresh fructus lycii 10 kilograms, after cleaning up, drains away the water with pure water, wears into fine and smooth homogenate with colloid mill; (2) load extractor, add the ethanol 100 kilograms that concentration is 55%; (3) under 45 DEG C of conditions, 10h is extracted; (4) with flame filter press filter, respectively clarified solution and filter residue for subsequent use; (5) clarified solution that (4) obtained is air-distillation 0.5h at 85 DEG C first, then vacuum be 0.1MPa, vapo(u)rizing temperature be 65 DEG C of conditions under decompression distillation 1h, obtain extract; (6) filter residue in (4) is added pure water 120 kg, be heated to 65 DEG C, adjusted to ph is 4.8, being set in mixer rotating speed is 60rpm, after extracting 80min, filters with 200 order industrial filter cloths under constantly stirring, respectively filtrate and filter residue for subsequent use, filter residue similarity condition repeats extraction 1 time, filters with 200 order industrial filter cloths, merges secondary filtrate, with centrifuge centrifugal 15min under the condition of 16000r/min, obtain centrifugate, then use 0.22 μm of filtering with microporous membrane, obtain filtrate; (7) filtrate is concentrated into heavy paste extract through thin film evaporation; (8) mix extract obtained to (5) with (7) are extract obtained, through vacuum drying, be ground into 500-700 order fine powder with airslide disintegrating mill; Adopt aluminum foil compound bag vacuum packaging, obtain wolfberry fruit extract product.
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