CN103734426A

CN103734426A - Health-care cornus officinalis tea capable of improving immunity and preparation method of tea

Info

Publication number: CN103734426A
Application number: CN201310749520.8A
Authority: CN
Inventors: 不公告发明人
Original assignee: Qingdao Hengbo Instrument Co Ltd
Current assignee: Jiashan Luoxing Venture Capital Co ltd
Priority date: 2013-12-31
Filing date: 2013-12-31
Publication date: 2014-04-23
Anticipated expiration: 2033-12-31
Also published as: CN103734426B

Abstract

The invention provides a health-care cornus officinalis tea capable of improving the immunity. The health-care cornus officinalis tea is prepared from the following raw materials in parts by weight: 10-50 parts of cornus officinalis, 10-20 parts of cortex lycii radicis, 10-20 parts of atractylodes macrocephala koidz, 10-20 parts of radix rehmanniae preparata, 10-20 parts of curculigo orchioides, 10-20 parts of epimedium, 10-20 parts of codonopsis pilosula, 10-20 parts of astragalus membranaceus, 10-20 parts of astragalus complanatus, 10-20 parts of fructus schizandrae, 10-20 parts of cortex albiziae, 10-20 parts of fructus rubi, 10-20 parts of poria cocos, 10-20 parts of radix polygoni multiflori preparata and 10-20 parts of asparagus cochinchinensis. The cornus officinalis can be used for tonifying the spleen, moistening the lung and reducing the lipid, reducing the blood fat, reducing the blood pressure, reducing the cholesterol, and reducing the blood sugar, so that the ingredients of the blood are purified. Traditional Chinese medicines which are mild in taste, and do not affect the taste of the cornus officinalis are added, and the cornus officinalis does not contain a foul and inedible taste of the traditional Chinese medicines, so that the cornus officinalis is easy to accept by people, can be drunk for a long period of time, and can well improve easiness in cold, eye acidity, poor sleep quality, spleen-insufficiency, hypodynamia, less eating and dry mouth, drinking and urine, kidney deficiency and soreness and weakness of the waist and the knees, frequent micturition at night, impotence and emission, tinnitus and blurring vision, premature graying of hairs, weakness, thinness, wind lecture and tinea of sub-health people.

Description

A kind of Fructus Corni health protection tea of improving immunity and preparation method thereof

Technical field

The present invention relates to contain the health products of the raising immunity that derives from plant composition, particularly a kind of Fructus Corni health protection tea of improving immunity and preparation method thereof.

Background technology

Human immunity is the defensive enginery of human body pathogenic microorganism to external world etc., or perhaps system of defense, and under normal circumstances, it can guarantee that human body is not subject to the infringement of extraneous pathogenic microorganism.Conventionally the body immunity of saying is resisted the ability of the infringements such as exotic disease pathogenic microorganism with regard to referring to the immune system of human body.In the normal situation of body immunity, human body self can be resisted the infringement of considerable germ, avoids human body to cause damage.Immunity plays an important role in human life mechanism, and nutrition of human body, fatigue, mood, environment, disease, medication, radiation etc. cause the immunity degradation of self.Over millions of years, the mankind are at environment that is not only applicable to existence but also is fraught with risks of life, and the mankind are able to survival, have also obtained outstanding immunity.Immunity is the product of biological evolution process thus.

The health of hypoimmunity is easy to infected or cancer stricken; Immunity is extraordinary also can produce insalubrious result, as caused allergic reaction, autoimmune disease etc.A variety of causes makes immune system normally not bring into play protective effect, in the case, very easily causes the infection such as bacterium, virus, fungi, and therefore the most directly to show be exactly liable to illness to hypoimmunity.Because of often ill, increased the weight of the consumption of body, thus generally have a delicate constitution, the performance such as malnutritive, One's spirits are drooping, fatigue and weak, appetite reduction, sleep-disorder, sick, have an injection and take medicine the normal potluck that just gets married.Each sickly all will could recover for a long time, and usually outbreak repeatedly.If things go on like this can cause health and intelligence development bad, also easily bring out major disease.Underlying causes is that hypoimmunity or immunity are unsound.When immune function of human body imbalance, or when immune system is unsound, following point will show effect repeatedly:--repeatedly show effect repeatedly, asthma is shown effect repeatedly, bronchitis is shown effect repeatedly, pneumonia is shown effect repeatedly by outbreak, tonsillitis for flu, the outbreak repeatedly of suffering from diarrhoea ... so necessarily can't neglect.

Immunity refers to that body resists external invasion and attack, safeguards the ability of homoiostasis.Various microorganisms in air, have been full of: bacterium, virus, mycoplasma, Chlamydia, fungi etc.In the situation that body immunity is low, they can become the morning pathogen of sense.Although human body can produce corresponding antibody to different pathogen; to resist subinfection again; but antibody have selectivity and time sex-limited, such as streptococcus antibody can only within compared with short-term, protect body be not subject to streptococcic again invade, also can not resist the infection of other viruses.The people of hypoimmunity cannot resist the invasion and attack of common cold virus at all, and this is only the true cause of the frequent flu of Susceptible population.

< < Compendium of Materia Medica > > carries: " Fructus Corni, cures mainly perverse trend fever and chills under the heart, in temperature, by cold warm numbness, goes three worms, and clothes are made light of one's life by commiting suicide for a long time; There are reinforcing yin essence benefit essence, five viscera settling, logical nine orifices, stop the merit of dribbling urination; Take for a long time improving eyesight, brute force all the year round ".Domestic Fructus Corni, Gu is had it by oneself.Business > > repaiied by clear Kangxu < < achievement and the total will > > in Shangzhou, Qianrong < < Zhili is all on the books, and titled with " good " word.Fructus Corni, has another name called cornus officinalis sieb et zucc, medicine jujube, real jujube, fructus corni, meat jujube etc., is the conventional rare traditional Chinese medicine of China, and applicating history is long.It is gentle with its benefit power, establishing-Yang and do not help fire, enriching yin and oiliness diaphragm, convergence and do not stay the evil special efficacy that waits by successive dynasties use that medical science is liked.Zhang Zhongjing has been formulated " chinkuei shin chewan pills " take Fructus Corni as monarch.According to chemical analysis, Fructus Corni contains the active ingredients such as cornin, tartaric acid, gallic acid, malic acid, resin, tannin and the multivitamin that physiologically active is stronger, having the immunity, anti-inflammatory of enhancing, the pharmacological action such as antibacterial, is the medicine simply of commonly using in tcm clinical practice.

The acid of Fructus Corni taste, adstringency, tepor.Return liver, kidney channel.Tonify the liver and kidney, puckery essence is admittedly de-.For dizzy tinnitus, soreness of waist and knee joint, impotence and seminal emission, enuresis frequent micturition, under uterine bleeding band, profuse sweating collapse, interior heat is quenched one's thirst, for astringent drug, it contains cornin, the not multiple effective ingredient such as the iridoid glycosides such as sieve glucoside, Cornuside, sweroside, loganin and saponin, ursolic acid, tartaric acid, gallic acid, malic acid, Fructus Corni tannin 1,2,3 of sieve glucoside, 7-O-methyl not.There are obvious diuresis, step-down, the antibacterial and leukocytic effect of elevating blood, diseases such as dizzy tinnitus to treatment due to liver-kidney deficiency, the soreness of waist and because the diseases such as the seminal emission due to the deficiency of the kidney yin, frequent micturition have obvious curative effects.Because having astringing to arrest bleeding effect, it is usually used in the menorrhalgia due to human female void again, the inferior disease of uterine bleeding band, the common disease of andrology, gynaecology is all had to good therapeutic action, a large amount of production for help medicine, therefore be listed in one of 40 kinds of large kinds of traditional Chinese medicines, have market widely at traditional Chinese medicine circle.

Fructus Corni, sour-puckery flavor, gas is flat, tepor, nontoxic.Enter kidney, liver two warps.The blood of temperature Liver Channel, the essence that kidney tonifying is dirty, Xing Yangdao is with firstborn's stem, warm waist knee and aid yang qi is adjustable through waiting, urine can be contracted, and water flowing key goes three worms, and brute force is prolonged life, the improving eyesight of making light of one's life by commiting suicide.Not use of its core, receives with involuntary emission is difficult, sage's pellet of real benefit the moon, the refreshing medicine of benefit marrow.So Zhong Jing Confucian scholar adopts in eight taste balls, get its controlling nocturnal emission with astringent drugs and unboiled water also.< < herbal classic > > calls its nine orifices and may lead to, and common people doubt person, too puckery with its taste, key closes and can not open, be probably difficult to lead to also.Give as not so.Madam's the five internal organs peace, nine orifices profit certainly, and within the five internal organs, a dirty uneasiness, four is dirty therefore uneasy.A so-called dirty person how be kidney also.Kidney is four dirty bases, and kidney is pacified and four dirty all peaces.Pacify four dirty and sharp nine orifices, what is doubted again.Fructus Corni is helped eight tastes with kidney tonifying, and Zhengan County's kidney with the medicine of five viscera settling also.The five internal organs were both pacified, and meaning nine orifices can not profit.And the more than sharp nine orifices of Fructus Corni also.Three warmers six internal organs, invariably mat its give shade, be subject to its grow benefit.Must use in this Eight-INgredients Decoction, and the newly vertical all sides of kidney tonifying of qi uncle Heavenly Teacher, that invariably uses hangs down exhausted disease also to rescue.

Fructus Corni has antidiabetic effect, cornusol extract has obvious hypoglycemic activity to alloxan and the sick rat of epinephrine glycosuria. and the diabetes rat that Streptozotocin (STZ) is formed also has similar effect. but normal rat blood sugar is had no significant effect, prompting Fructus Corni has certain therapeutic action to IDD patient. and experiment shows: Fructus Corni pulvis, ether extract and the ursolic acid further separating all can reduce blood sugar significantly, glucose in urine, amount of drinking water and the amount of urinating, illustrate that ursolic acid is the antidiabetic active component of Fructus Corni. there is report to point out, with rat epididymis adipose tissue, to test and find that Fructus Corni has ILA. Fructus Corni tannic acid can suppress lipid peroxidation, stop lipolysis, also can suppress adrenaline and cortex hormone of aadrenaline and promote fat-splitting effect.

Experiment in vitro shows: cornus fruit decoction can suppress the growth of staphylococcus aureus, but invalid to Escherichia coli. decoction (1: 1) has inhibitory action to shigella, its antibacterial circle diameter can reach 11～18mm (dull and stereotyped ring agar diffusion method). and also there is people from fresh dogwood fruits meat, to obtain a kind of dark red liquid, experiment shows that it is to typhoid bacillus, shigella dysenteriae has inhibitory action. and Fructus Corni water logging agent (1: 3) is in vitro to trichophyton, Trichophyton concentricum, permitted blue achorion, Du's big belly microspore tinea bacterium difficult to understand, rust tinea bacterium, ulotrichy microspore's tinea bacterium, epidermophyton inguinale, epidermophyton rubrum, Kaufman-Wolf Epidermophyton, the dermatophytes such as star-shaped nocardia all have inhibitory action in various degree.

Chinese medicine odor poverty, a lot of people are reluctant long-term taking; Even if take, be three points of poison of medicine, while allowing people's long-term taking, also produce resisting psychology.How the efficacy exertion of Fructus Corni not being injured again to human body to maximum, and obviously improve mouthfeel, people can be accepted, is our problem in the urgent need to address.Active material health products are safely and effectively contained in a large number in Fructus Corni the inside, can greatly improve immunity, are that people expect the key of dealing with problems always.That Chinese medicine Fructus Corni has advantages of is nontoxic, can be absorbed completely by organism, is the main direction that we study.Now market in the urgent need to develop a kind of nontoxic, can be absorbed completely by organism, greatly improve the health products of the high-quality of body immunity.

Summary of the invention

Technical problem to be solved by this invention is, it is more and more fragile that modern's immunity is just becoming, environmental pollution, work and rest irregular, rhythm of life fast, eat unhealthy, do not know continence, cause the incidence of disease of various diseases all in continuous rising.Especially gang of so-called office, the especially group of people at high risk of hypoimmunity.Most people are sub-health states, are overdrawing originally with regard to unsound health.Most people's symptom is insomnia and dreamful sleep, easy catching a cold, eyes are sour and astringent, insufficiency of the spleen weak; The few dry of food; Quench one's thirst; Deficiency of the kidney soreness and weakness of waist and knees; Impotence and seminal emission; Tinnitus order is dark; Poliosis; The empty win of body is thin; Wind favus disease.How to find active material health products safely and effectively, can greatly improve immunity, build up health is that people expect the key of dealing with problems always.Fructus Corni has hypotensive, hypoglycemic, reducing blood lipid, and atherosclerosis, delays senility and effect such as antibacterial grade.The acid of Fructus Corni taste, adstringency, tepor.Return liver, kidney channel.Tonify the liver and kidney, puckery essence is admittedly de-.For dizzy tinnitus, soreness of waist and knee joint, impotence and seminal emission, enuresis frequent micturition, under uterine bleeding band, profuse sweating collapse, interior heat is quenched one's thirst, for astringent drug, it contains cornin, the not multiple effective ingredient such as the iridoid glycosides such as sieve glucoside, Cornuside, sweroside, loganin and saponin, ursolic acid, tartaric acid, gallic acid, malic acid, Fructus Corni tannin 1,2,3 of sieve glucoside, 7-O-methyl not.There are obvious diuresis, step-down, the antibacterial and leukocytic effect of elevating blood, diseases such as dizzy tinnitus to treatment due to liver-kidney deficiency, the soreness of waist and because the diseases such as the seminal emission due to the deficiency of the kidney yin, frequent micturition have obvious curative effects.Because it has astringing to arrest bleeding effect, be usually used in the menorrhalgia due to human female void again, the inferior disease of uterine bleeding band, all has good therapeutic action to the common disease of andrology, gynaecology, in a large number for the production of help medicine.Add some mouthfeels light, do not affect the Chinese medicine of Fructus Corni taste, do not affect mouthfeel and make people be easy to accept, can reach preferably the effect that improves immunity.Chinese medicine odor poverty, a lot of people are reluctant long-term taking; Even if take, be three points of poison of medicine, while allowing people's long-term taking, also produce resisting psychology.How the efficacy exertion of Fructus Corni not being injured again to human body to maximum, and obviously improve mouthfeel, people can be accepted, is our problem in the urgent need to address.

For solving above-mentioned technical barrier, the invention provides a kind of Fructus Corni health protection tea of improving immunity, the parts by weight of the various raw materials of described health protection tea are: 10～50 parts of Fructus Cornis, 10～20 parts of the root barks of Chinese wolf-berry, 10～20 parts of the bighead atractylodes rhizomes, 10～20 parts, cultivated land, 10～20 parts of thizoma curculiginises, 10～20 parts of barrenwort, 10～20 parts of Radix Codonopsis, 10～20 parts of the Radixs Astragali, 10～20 parts of semen astragali complanatis, 10～20 parts, the fruit of Chinese magnoliavine, 10～20 parts of cortex albiziaes, 10～20 parts of raspberries, 10～20 parts, Poria cocos, 10～20 parts of RADIX POLYGONI MULTIFLORI PREPARATAs, 10～20 parts of lucid asparagus.

The Fructus Corni health protection tea of described raising immunity, described in it, the parts by weight of the various raw materials of smart health protection tea can be: 10～30 parts of Fructus Cornis, 10～15 parts of the root barks of Chinese wolf-berry, 10～15 parts of the bighead atractylodes rhizomes, 10～15 parts, cultivated land, 10～15 parts of thizoma curculiginises, 10～15 parts of barrenwort, 10～15 parts of Radix Codonopsis, 10～20 parts of the Radixs Astragali, 10～20 parts of semen astragali complanatis, 10～20 parts, the fruit of Chinese magnoliavine, 10～20 parts of cortex albiziaes, 10～20 parts of raspberries, 10～20 parts, Poria cocos, 10～20 parts of RADIX POLYGONI MULTIFLORI PREPARATAs, 10～20 parts of lucid asparagus.

The Fructus Corni health protection tea of described raising immunity, described in it, the parts by weight of the various raw materials of health protection tea can also be: 10～40 parts of Fructus Cornis, 10～20 parts of the root barks of Chinese wolf-berry, 10～20 parts of the bighead atractylodes rhizomes, 10～20 parts, cultivated land, 10～20 parts of thizoma curculiginises, 10～20 parts of barrenwort, 10～20 parts of Radix Codonopsis, 10～15 parts of the Radixs Astragali, 10～15 parts of semen astragali complanatis, 10～15 parts, the fruit of Chinese magnoliavine, 10～15 parts of cortex albiziaes, 10～15 parts of raspberries, 10～15 parts, Poria cocos, 10～15 parts of RADIX POLYGONI MULTIFLORI PREPARATAs, 10～15 parts of lucid asparagus.

For solving the problems of the technologies described above, the present invention also provides the preparation method of a kind of Fructus Corni health protection tea of improving immunity, and described in it, the preparation process of Fructus Corni health protection tea comprises following aspect:

A. the preparation of Fructus Corni;

B. by described other raw material pulverizing, immersion, heating, refluxing extraction 2 times;

C. the liquid again other raw materials being extracted is condensed into granule, pulvis or paste;

D. concentrated granule, pulvis or paste are mixed with the Fructus Corni preparing, be prepared into health care medicinal tea.

In described step a, picking fruit when the time of the year when autumn changes into winter, Fructus Corni pericarp reddened, after drying or put in boiling water and slightly to scald with slow fire, in time except removal core, dry, dry raw dogwood fruit and put a pot interior water proof steam 30-60 minute at 80-100 ℃, steam to puce, then dry.

In described step b, surplus stock can be cleaned to impurity elimination, steep into ethanol, heating and refluxing extraction 2 times, each 1～2 hour, merges standing by No. 2 extracts; Above-mentioned two kinds of extracts are merged, and decompression recycling ethanol to be concentrated into liquor strength be 0.6g crude drug/mL, after suction filtration, when the relative density of filtrate is about 20 ℃ 1.08; Be decompressed to 0.03-0.08MPa, temperature remains on 60-80 ℃, and being concentrated into relative density is 1.20, the medicinal extract that temperature to 60 is ℃-70 ℃.

In described step c, the medicinal extract that above-mentioned steps can be extracted be inserted in economic benefits and social benefits vacuum concentrator, the concentrate that while being concentrated into 90 ℃, relative density is 1.05, set to 0～5 ℃ of deepfreezes 24 hours; Refrigeration liquid is added to 0.3% filter aid diatomite, filter, filtrate is inserted in economic benefits and social benefits vacuum concentrator again, is concentrated into every 1ml containing 1g crude drug amount, then adds dextrin and puts into granulator and granulate.

In described steps d, the particle that the Fructus Corni preparing can be prepared with other raw materials mixes, and ultraviolet disinfection, packs and get final product.

For solving the problems of the technologies described above, the present invention also provides the preparation method of a kind of Fructus Corni health protection tea of improving immunity, when other raw materials of described raising immunity Fructus Corni health protection tea are made pulvis, step is: other raw materials are put into 5-10 times of amount ethanol, soak 1-2 hours, heating is extracted 2 times, each 1-2 hours, remove supernatant, merge extract, 100-120 orders filter, through the molecular weight that dams, be 5000-10000 ultrafiltration post ultrafiltration again, 1.36 medicinal extract when ultrafiltrate reduced pressure concentration relative density is 80 ℃, then put into drying baker and be dried into powder, after ultraviolet radiation for sterilizing and disinfecting, merge packing with the Fructus Corni preparing.

For solving the problems of the technologies described above, when the present invention provides other raw materials that improve immunity Fructus Corni health protection tea described in the preparation method of a kind of Fructus Corni health protection tea of improving immunity to make paste again, step is: other raw materials are put into 5-10 times of amount ethanol, soak 1-2 hours, heating is extracted 2 times, each 1-2 hours, remove supernatant, merge extract, 100-120 orders filter; The dregs of a decoction of other bulk drugs of more above-mentioned alcohol extract being crossed add 10 times of water gaging heating and refluxing extraction 2 times, each 1～2 hour, No. 2 extracts are merged standing; Above-mentioned two kinds of extracts are merged, and decompression recycling ethanol, and to be concentrated into liquor strength be 0.6g crude drug/mL, after suction filtration, when the relative density of filtrate is about 20 ℃ 1.08; Be decompressed to 0.03-0.08MPa, temperature remains on 60-80 ℃, and being concentrated into relative density is 1.20, the medicinal extract that temperature to 60 is ℃-70 ℃.

The technique effect that the present invention is useful is, that the present invention has advantages of is nontoxic, can be absorbed completely by organism.Fructus Corni has hypotensive, hypoglycemic, reducing blood lipid, and atherosclerosis, delays senility and effect such as antibacterial grade.The acid of Fructus Corni taste, adstringency, tepor.Return liver, kidney channel.Tonify the liver and kidney, puckery essence is admittedly de-.For dizzy tinnitus, soreness of waist and knee joint, impotence and seminal emission, enuresis frequent micturition, under uterine bleeding band, profuse sweating collapse, interior heat is quenched one's thirst, for astringent drug, it contains cornin, the not multiple effective ingredient such as the iridoid glycosides such as sieve glucoside, Cornuside, sweroside, loganin and saponin, ursolic acid, tartaric acid, gallic acid, malic acid, Fructus Corni tannin 1,2,3 of sieve glucoside, 7-O-methyl not.There are obvious diuresis, step-down, the antibacterial and leukocytic effect of elevating blood, diseases such as dizzy tinnitus to treatment due to liver-kidney deficiency, the soreness of waist and because the diseases such as the seminal emission due to the deficiency of the kidney yin, frequent micturition have obvious curative effects; Add some promoting blood circulation and removing blood stasis, the Chinese medicine of invigorating spleen to remove dampness yin-nourishing, the most mouthfeel of these medicinal materials is light, do not affect the taste of Fructus Corni tea, there is no the unpleasant taste of traditional Chinese medicine of swallowing of being difficult to, make people be easy to accept, can long-term taking, can improve preferably sub-health population easy catching a cold, eyes are sour and astringent, sleep quality is not high, insufficiency of the spleen weak; The few dry of food; Quench one's thirst; Deficiency of the kidney soreness and weakness of waist and knees; Impotence and seminal emission; Tinnitus order is dark; Poliosis; The empty win of body is thin; The symptom of wind favus disease, can reach the effect that improves immunity preferably.

The specific embodiment

Human immunity is the defensive enginery of human body pathogenic microorganism to external world etc., or perhaps system of defense, and under normal circumstances, it can guarantee that human body is not subject to the infringement of extraneous pathogenic microorganism.Conventionally the body immunity of saying is resisted the ability of the infringements such as exotic disease pathogenic microorganism with regard to referring to the immune system of human body.In the normal situation of body immunity, human body self can be resisted the infringement of considerable germ, avoids human body to cause damage.Immunity plays an important role in human life mechanism, and nutrition of human body, fatigue, mood, environment, disease, medication, radiation etc. cause the immunity degradation of self.More and more research shows, Chinese medicine more and more plays a significant role to the adjusting of body immunity.

Fructus Corni can suppress the effect of platelet aggregation: dogwood fruit injecting fluid external administration, can obviously suppress the rabbit platelet aggregation of threshold concentration adenosine diphosphate (ADP) (ADP) sodium salt, collagen or arachidonic acid-induction, inhibitory action strengthens and strengthens with its consumption, and dosage is relevant to effect; Intravenously administrable also shows that it can suppress the rabbit platelet aggregation of ADP induction, illustrate that entirety is consistent with isolated test result. in addition, the thrombus that dogwood fruit parenteral solution can also suppress Rat common carotid-vena jugularis externa bypass circuit generates. suppress platelet aggregation, antithrombotic formation, to alleviating DIC, being formed with definite meaning, being conducive to the treatment to shock. this is also one of mechanism of dogwood fruit treatment shock.

Fructus Corni has the effect of killing ascites cells in vitro. also there is the effect of anti-experimental character hepatic lesion. the leucocyte causing because of chemotherapy and radiotherapy is declined, there is the effect that makes its rising. cornin toxicity is very low, not haemolysis, but have the weak parasympathetic effect of excitement .6. scapulohumeral periarthritis (scapulohumeral periarthritis): 35 grams of Fructus Cornis.Decocting divides clothes, 1 dose of every day 2 times.After sb.'s illness took a favorable turn, dosage is kept to 10～15 grams, decocts soup or is taken after being infused in hot water or decoction for tea.There is good curative effect, generally take medicine 4～5 doses and just start to take effect.The empty hidrosis of body: each 15 grams of Fructus Corni, Radix Codonopsis, 9 grams, the fruit of Chinese magnoliavine.Be decocted in water for oral dose, 1 dose of every day.To the empty hidrosis of body, the person that easily do not catch a cold is effective.

Radix Codonopsis is the conventional traditional tonic of China, and the Radix Codonopsis that produce ancient times take area, Shangdang, Shanxi, as top grade, has tonifying middle-Jiao and Qi, the effect of invigorating the spleen benefit lung.Modern study, Radix Codonopsis, containing various saccharides, phenols, sterol, volatile oil, scutellarein glucosid, saponin and Alkaloid, has immunity, hemangiectasis, the step-down of enhancing, improves microcirculation, strengthens the effects such as hematopoiesis function.In addition leucocyte chemotherapy radiotherapy being caused declines castering action.Radix Codonopsis has tonifying middle-Jiao and Qi, the effect of invigorating the spleen benefit lung.For a little less than the spleen deficiency syndrome of the lung, the palpitaition of breathing hard, anorexia and loose stool, void is breathed with cough and is coughed, and interior heat is quenched one's thirst etc.< < new compilation of materia medica > > records: " tonifying middle-Jiao and Qi and taste, relieving thirst and restlessness.Middle gas is faint, in order to adjust, mends, very smooth and proper.”

Poria cocos taste is sweet, light, property is flat, be used as medicine there is clearing damp and promoting diuresis, the function of beneficial spleen and stomach, antitoxic heart-soothing and sedative.Modern medicine study: Poria cocos energy enhanced machine body immunity function, pachymaran has significantly antitumor and protect the liver dirty effect.Poria cocos slightly sweet flavor is flat, enters the heart, lung, the spleen channel.There is eliminating dampness and diuresis, strengthening the spleen and stomach, the effect of antitoxic heart-soothing and sedative.Can control difficult urination, oedema turgor, phlegm and retained fluid is coughed contrary, vomits contrary, and vomiting during early pregnancy, has loose bowels, seminal emission, stranguria with turbid discharge, palpitation with fear, forgetful disease such as grade.The Li Shui of Poria cocos, reaches by strong fortune spleen PFT, different from the Chinese medicine of other direct Li Shui.For splenasthenic diarrhea, the lower Poria cocos of band can invigorating the spleen, again can excreting dampness, and due to insufficiency of the spleen dysfunction of the spleen in transportation and transformation, to have loose bowels, be with down, application Poria cocos has the effect of giving consideration to both the incidental and fundamental, the compatibilities such as normal and Radix Codonopsis, the bighead atractylodes rhizome, Chinese yam.Can be used as tonifying lung spleen, control the adjuvant of the deficiency of vital energy.

Fruit of Chinese magnoliavine temperature; Sour, sweet; Return lung, the heart, kidney channel; Restrain astringent or styptic treatment for spontaneous sweating, nourishing generate fluid, kidney calming.For the void of coughing for a long time, breathe heavily, emission, enuresis frequent micturition, endless diarrhea, spontaneous perspiration, night sweat, Tianjin wound is thirsty, the feeble pulse of losing heart, interior heat is quenched one's thirst, palpitation and insomnia.< < Bencao Jingshu > > carries: the main beneficial gas person of the fruit of Chinese magnoliavine, and all gas of lung master, acid can be received, and is just entering lung tonifying lung, therefore beneficial gas is also.Its main cough with dyspnea person, the deficiency of vital energy is above stopped up and is not returned unit, and sour flavor having the property of astringing, takes the photograph gas and returns unit, and cough with dyspnea is from removing.Internal lesion caused by overexertion is won thin, tonifying for the deficiency, reinforcing yin essence, beneficial man's essence.< < does not record > > and supports the five internal organs, heat extraction, flesh person in raw the moon, the special kidney tonifying of the fruit of Chinese magnoliavine, the tonifying five zang organs of holding concurrently, kidney storing essence, essence is contained cloudy strong, receipts are taken the photograph vital qi and are returned unit, and public region is warm, digesting food, steam waste matter and change precise and tiny, smart spontaneous, essence is raw cloudy long, therefore main as above all diseases also.

Cultivated land has another name called prepared rhizome of rehmannia or throws oneself on the ground, belong to goatweed, be a kind of first-class Chinese medicine, there is the enriching yin of enriching blood effect, can be used for the deficiency of blood sallow, dizzy, palpitation and insomnia, irregular menstruation, the diseases such as uterine bleeding, also can be used for hectic fever hectic fever due to yin, the night sweat of the deficiency of the kidney yin, the disease such as pass out semen, quench one's thirst, is one of asthenic symptoms class non-prescribed medicine medicine Liuwei Dihuang Wan main component.This quality is moistened into kidney, kind nourishing kidney yin, and life essence-filling, marrow-benefitting, is the key medicine of tonifying kidney-yin.Ancients call it " large tonifying five zang organs is Kidney-Yin ", " mending greatly true water ".Normal be equal to use with Chinese yam, Fructus Corni, treat the deficiency of liver-yin and kidney-yin, soreness and weakness of waist and knees, seminal emission, night sweat, tinnitus, deafness and quench one's thirst etc., can filling liver kidney, and beneficial marrow, as Liuwei Dihuang Wan.

The Radix Astragali has the effects such as invigorating qi for strengthening superficies, sharp water detumescence, pus draining and toxin expelling, myogenic.The medicinal history of existing more than 2000 year so far of the Radix Astragali, modern study, the Radix Astragali is containing various trace elements such as saponin, sucrose, polysaccharide, several amino acids, folic acid and selenium, zinc, copper.Have enhanced machine body immunity function, protect the liver, diuresis, anti-ageing, anti-stress, step-down and antibacterial action more widely.But table is real evil, contain, retention of dampness due to stagnation of QI, dyspepsia is stagnated, ulcer from the beginning of or the real example such as routed after heat poison Shang Sheng.The Radix Astragali has invigorating qi for strengthening superficies, diuresis holder poison, apocenosis, the effect of expelling pus and promoting granulation.Weak for the deficiency of vital energy, anorexia and loose stool, the sinking of qi of middle-jiao, rushes down prolapse of the anus for a long time, the uterine bleeding of having blood in stool, exterior deficiency spontaneous perspiration, ulcer is difficult bursts, and bursts and does not hold back for a long time, and the deficiency of blood is sallow, and interior heat is quenched one's thirst.< < herbal classic > > records: " main ulcer, loses sore for a long time, apocenosis pain relieving.Qi-restoratives, children's's all kinds of diseases and ailments." < < Japan hanako materia medica > > record: " help gas to strengthen the bone, put on flesh and enrich blood.”

RADIX POLYGONI MULTIFLORI PREPARATA be by Radix Polygoni Multiflori and black soya bean with the tuber of multiflower knotweed drying after boiling, be filling liver kidney simply, benefiting essence-blood, the good medicine that nourishes heart and allay excitement.Be mainly used in treatment and because of kidney deficiency and liver, essence and blood, lose the diseases such as the soreness and weakness of waist and knees that causes, dizzy, palpitation and insomnia, graying of hair.It is to bring into play by kidney tonifying, benefit essence, the effect of allaying excitement of nourishing blood that the crow of the fleece-flower root sends out function.RADIX POLYGONI MULTIFLORI PREPARATA has enrich and benefit essence and blood, and nourishing the liver is calmed the nerves, strengthening the bones and muscles, and the crow palpus that reinforces the kidney, is shown in Light-headedness for the deficiency of blood, palpitation and insomnia is forgetful, sallow weak, the dizzy tinnitus of liver kidney blood and essence asthenia, soreness and weakness of waist and knees, the diseases such as band, poliosis are collapsed in seminal emission.

The root bark of Chinese wolf-berry is cold in nature, and taste is sweet.Function cures mainly: cool blood is except steaming, and fire falls in clearing lung-heat.For deficiency of Yin hectic fever, hectic fever due to yin night sweat, cough with lung heat, spitting of blood, bleeding from five sense organs or subcutaneous tissue.< < does not record > >: " main air is wet, lower breast side of body gas, the headache of visitor's heat, mends internal injury great Lao suctions of hissing, hard muscle, reinforcing yin essence, sharp intestine and small intestine, heat resists cold.< < book on Chinese herbal medicine is stated > >: " cure mainly deficient fever, alternative fever and chill, is allly shown in blood trouble, nosebleed epistaxis, cough blood; cough, breathe heavily, the illness that disappears, apoplexy; dizzy, convulsion is chronic, pain in the back; migratory arthralgia, and beriberi, oedema; dysphoria, throb with fear, forgetful; urinary obstruction, reddish and whitish turbid urine.″

The bighead atractylodes rhizome can strengthen shrinkage amplitude, the contraction frequency of intestinal smooth muscle significantly, extends ST under anaerobic conditions of intestinal smooth muscle, adjusts gastrointestinal motility function, has antiulcer, protects the liver and the effect of enhanced machine body immunity function.Bighead atractylodes rhizome bitter, sweet, temperature.Return spleen, stomach warp.Cure mainly strengthening the spleen and replenishing qi, eliminating dampness Li Shui, hidroschesis, antiabortive.Few for insufficiency of the spleen food, abdominal distension is had loose bowels, and phlegm and retained fluid is dizzy throbs with fear, oedema, spontaneous perspiration, fetal irritability.The bighead atractylodes rhizome has CDCC to oncocyte, can reduce the rate of increase of oncocyte, lowers the aggressive of tumor tissue, improves the ability of the antitumor reaction of body.< < property of medicine opinion > > carries it: monarch, taste is sweet, pungent, nontoxic.The large wandering arthritis of energy master, diarrhea due to disorder of qi for many years, trusted subordinate's distending pain, the broken place food that disappears, appetizing, removes sputum, removes fever and chills, under stopping, lets out.Interarea light is happy, preserves youthful looks, and de-black.The swelling of harnessing the river is full, tells to vomit contraryly, and crymodynia in abdomen, vomits and diarrhoea incessantly, and the cold dysentery of stomach Qi deficiency.

Thizoma curculiginis taste is sweet, and property is flat.Return lung; Stomach warp.Nourishing Yin and moistening lung; Nourishing the stomach to improve the production of body fluid.Cough caused by dryness; Labor is coughed; The dry throat and mouth of the cloudy liquid consumption of pyreticosis wound; Interior heat is quenched one's thirst; Deficiency of Yin diseases caused by external factors; Dizzy dizzy; Muscle arteries and veins contraction pain.The sweet greasiness of thizoma curculiginis taste, matter is soft and moisten, and is the good medicine of nourishing Yin and promoting production of body fluid simply.Contained vitamin A in thizoma curculiginis, improves dry and cracked, rough skin situation, makes it soft lubricated.Play the effect of beauty and skin care.< < detailed outline > >: " main air temperature spontaneous perspiration is scorching hot, and malaria with general debility fever and chills, and taste void is weary, and man's frequent urination is lost essence, and all are deficient.”

Cortex albiziae nature and flavor are sweet, flat.There is the merit of Xie Yu and blood, the peaceful heart, subduing inflammation.Have control that confused and worried, melancholy, insomnia, lung carbuncle, carbuncle are swollen, the effect of scrofula, injured bone.< < Sheng Nong's herbal classic > >: " silk tree, taste is sweet flat.Main five viscera settling, sharp will, makes us happy carefree ... raw mountain valley." cortex albiziae is the skin of legume silk tree.Xia Qiushicai bark, dries medicinal.Nature and flavor are sweet, flat.There is the merit of Xie Yu and blood, the peaceful heart, subduing inflammation.Have control that confused and worried, melancholy, insomnia, lung carbuncle, carbuncle are swollen, the effect of scrofula, injured bone.

Raspberry is warm in nature; Sweet, sour; Return kidney, urinary bladder channel.Cure mainly: kidney-nourishing, controlling nocturnal emission with astringent drugs, contracting urine.For enuresis with renal asthenia, frequent urination, impotence and premature ejaculation, emission and spermatorrhea.< < Bencao Jingshu > >: " raspberry, its main beneficial gas person, says beneficial vital essence also.Kidney storing essence, kidney are received gas, vital essence abundance, and body, from light, is sent out in vain not also.The respectful main qi-restoratives of reviving is absolutely continuous, reinforcing yin essence Jianyang, smoothing skin, peace and internal organs.The main man's kidney-jing deficiency of Zhen Quan exhausts, impotence, woman's food have a son.Large bright main five viscera settling, beneficial color, supports vital essence, long hair, strong will.All get its kidney-nourishing and add essence, the adopted ear of sweet acid convergence." < < property of medicine opinion > > carries it: " main man's kidney-jing deficiency exhausts, woman's food have a son.Main impotence.″

Barrenwort, excessive sheep flower bud is Chinese conventional Chinese medicine, has very high medical value, herb hyoscine.Cure mainly impotence and premature ejaculation, pain in low back and legs, numb limb, hemiplegia, neurasthenia, forgetful, tinnitus, dizzy disease such as grade.

Lucid asparagus, Latin literary fame Radix Asparagi. has another name called asparagus fern.Cold in nature, taste is sweet, micro-hardship.There is replenishing the vital essence and removing heat, the effect of moistening lung nourshing kidney.Be used for controlling fever due to yin deficiency, cough haematemesis, lung carbuncle, abscess of throat, quench one's thirst, the illness such as constipation.Originating in South Africa, is the common cultivations in China various places.

Semen astragali complanati tonifying liver, kidney-nourishing, improving eyesight, controlling nocturnal emission with astringent drugs.Control kidney deficiency and liver, soreness of waist and knee joint, blurred vision, seminal emission premature ejaculation, frequent urination, the enuresis, hematuria, leukorrhea.< < Amplification on Materia Medica > >: kidney tonifying.< < detailed outline > >: kidney tonifying, control pain in the back and let out essence, deficient tired.

Specific embodiment 1:

First prepared by Fructus Corni.

Again by all the other raw material root barks of Chinese wolf-berry, the bighead atractylodes rhizome, cultivated land, thizoma curculiginis, barrenwort, Radix Codonopsis, the Radix Astragali, semen astragali complanati, the fruit of Chinese magnoliavine, cortex albiziae, raspberry, Poria cocos, RADIX POLYGONI MULTIFLORI PREPARATA, lucid asparagus, extracts concentrated granulation agent, pulvis or paste.

The extract particles agent of the Fructus Corni preparing and other raw materials, ultraviolet radiation for sterilizing and disinfecting pack, directly can take after mixing it with hot water during use.

Specific embodiment 2:

Get picking fruit when the time of the year when autumn changes into winter, Fructus Corni pericarp reddened, approximately 5000g, after drying or put and slightly scald in boiling water with slow fire, in time except removal core, dry, dry raw dogwood fruit and put a pot interior water proof steam 30-60 minute at 80-100 ℃, steam to puce, then dry; By surplus stock root bark of Chinese wolf-berry 1500g, bighead atractylodes rhizome 1500g, cultivated land 1500g, thizoma curculiginis 1500g, barrenwort 1500g, Radix Codonopsis 1500g, Radix Astragali 1500g, semen astragali complanati 1500g, fruit of Chinese magnoliavine 1500g, cortex albiziae 1500g, raspberry 1500g, Poria cocos 1500g, RADIX POLYGONI MULTIFLORI PREPARATA 1500g, lucid asparagus 1500g, cleans impurity elimination, steeps into ethanol, heating and refluxing extraction 2 times, each 1～2 hour, merges standing by No. 2 extracts; Above-mentioned two kinds of extracts are merged, and decompression recycling ethanol to be concentrated into liquor strength be 0.6g crude drug/mL, after suction filtration, when the relative density of filtrate is about 20 ℃ 1.08; Be decompressed to 0.03-0.08MPa, temperature remains on 60-80 ℃, and being concentrated into relative density is 1.20, the medicinal extract that temperature to 60 is ℃-70 ℃.The medicinal extract that above-mentioned steps is extracted is inserted in economic benefits and social benefits vacuum concentrator, the concentrate that while being concentrated into 90 ℃, relative density is 1.05, set to 0～5 ℃ of deepfreezes 24 hours; Refrigeration liquid is added to 0.3% filter aid diatomite, filter, filtrate is inserted in economic benefits and social benefits vacuum concentrator again, is concentrated into every 1ml containing 1g crude drug amount, then adds dextrin and puts into granulator and granulate.The particle that the Fructus Corni preparing is prepared with other raw materials mixes, and ultraviolet disinfection is packed and be get final product.

Specific embodiment 3:

Get picking fruit when the time of the year when autumn changes into winter, Fructus Corni pericarp reddened, approximately 3000g, after drying or put and slightly scald in boiling water with slow fire, in time except removal core, dry, dry raw dogwood fruit and put a pot interior water proof steam 30-60 minute at 80-100 ℃, steam to puce, then dry; By surplus stock root bark of Chinese wolf-berry 1200g, bighead atractylodes rhizome 1200g, cultivated land 1300g, thizoma curculiginis 1200g, barrenwort 1200g, Radix Codonopsis 1300g, Radix Astragali 1200g, semen astragali complanati 1100g, fruit of Chinese magnoliavine 1200g, cortex albiziae 1200g, raspberry 1200g, Poria cocos 1200g, RADIX POLYGONI MULTIFLORI PREPARATA 1200g, lucid asparagus 1300g, puts it in 10 times of water gagings and soaks.Drop into multi-function extractor and extract twice, add for the first time 8-10 times of water gagings and decoct 1-2 hours, add for the second time 4-8 times of water gagings and decoct 1-2 hours, merge twice decocting liquid, filter to get filtrate; Heating is concentrated into powder, then puts into baking oven inner drying powdered, and hybrid packed with the good lucid asparagus of frying after ultraviolet radiation for sterilizing and disinfecting, during use, direct boiling water is taken after mixing it with water.

Specific embodiment 4:

Get picking fruit when the time of the year when autumn changes into winter, Fructus Corni pericarp reddened, approximately 3000g, after drying or put and slightly scald in boiling water with slow fire, in time except removal core, dry, dry raw dogwood fruit and put a pot interior water proof steam 30-60 minute at 80-100 ℃, steam to puce, then dry; By surplus stock root bark of Chinese wolf-berry 1200g, bighead atractylodes rhizome 1200g, cultivated land 1300g, thizoma curculiginis 1200g, barrenwort 1200g, Radix Codonopsis 1300g, Radix Astragali 1200g, semen astragali complanati 1100g, fruit of Chinese magnoliavine 1200g, cortex albiziae 1200g, raspberry 1200g, Poria cocos 1200g, RADIX POLYGONI MULTIFLORI PREPARATA 1200g, lucid asparagus 1300g.Other raw materials are put into 5-10 times of amount ethanol, soak 1-2 hours, heating is extracted 2 times, and each 1-2 hours, remove supernatant, merge extract, 100-120 orders filter; The dregs of a decoction of other bulk drugs of more above-mentioned alcohol extract being crossed add 10 times of water gaging heating and refluxing extraction 2 times, each 1～2 hour, No. 2 extracts are merged standing; Above-mentioned two kinds of extracts are merged, and decompression recycling ethanol, and to be concentrated into liquor strength be 0.6g crude drug/mL, after suction filtration, when the relative density of filtrate is about 20 ℃ 1.08; Be decompressed to 0.03-0.08MPa, temperature remains on 60-80 ℃, and being concentrated into relative density is 1.20, the medicinal extract that temperature to 60 is ℃-70 ℃.

Pharmacology toxicity test

Experimental example 1: acute toxicity test of the present invention

One, test material: animal: Kunming mouse, body weight 18-25g, male and female half and half, Shandong University's biology laboratory breeding.Medicine: the present invention's (all raw material mixes decoction 2 times, merges and filters, taking liquid) is containing 0.0365mg/ml.

Two, method:

1, LD50 calculates: adopt improvement karber's method, mouse is divided into 5 groups at random, 10 every group, male and female half and half, dissolve adding distil water of the present invention, are made into Cmax, by the administration of the maximum permission capacity of mouse, institute is followed successively by 18,14.4 to dosage by crude drug amount, 11.5,9.2,7.4 (g.kg-1), in animal fasting (can't help water) after 18 hours, administration at twice in one day (interval half an hour), each 0.5ml, observes animal dead situation.

2, maximum tolerated dose is measured (MTD value): get 20 of mouse, each 10 of male and female.Adding distil water of the present invention is dissolved, be made into maximum concentration, by the maximal tolerance dose of animal, to inject, fill with hello device and can twitch and be as the criterion.In animal fasting (can't help water) after 18 hours, administration at twice in one day (interval half an hour), each 0.5ml (every ml is containing crude drug 0.36g), total dose is 18g crude drug/kg.d, 300 times of quite clinical adult 50Kg body weight consumption.Continuous Observation 7 days after administration.

Three, result of the test:

LD50 calculate in when giving mouse with maximum acceptable concentration and maximum permission capacity (18g/Kg.d), have no dead mouse, do not measure LD50, ask only maximum tolerated dose, in 7 day observation period, its appetite of animal, activity, hair color, the state of mind etc. are all normal, grow normally, there are no death.Select 300 times of doses that are equivalent to clinical dosage, and have no adverse reaction, show that acute toxicity is minimum, MTD>18g/Kg.d.

Experimental example 2: the result of the test of acute toxicity and long term toxicity

Acute toxicity test: give the present invention by the disposable gavage of small white mouse, maximum concentration 35%, maximum gavage capacity 0.4ml/10g, dosage 14g/kg (every g medicinal powder is equivalent to 10g crude drug), Continuous Observation 7 days, do not find any toxic reaction, because concentration and dosage cannot increase, therefore fail to measure the LD50 of this medicine.Maximum tolerance determination: with maximum concentration, maximum gavage capacity, mice lavage administration 3 times, 5 hours, interval, then Continuous Observation 7 days, none example is dead.Medicinal powder dosage is >42g/kg. day (every g medicinal powder is equivalent to 10g crude drug), by kg body weight, calculate the clinical consumption per day that is equivalent to be grown up 420 times.

Long term toxicity test: whether produce toxic reaction for observing long-term prescription, give respectively rat the present invention and raise clothes, by adult 70 times of clinical consumption per day and 35 times (being 7g/kg/ day and 3.5g/kg/ day), to give and feed 8 weeks continuously, the behavior, feed, the body weight that have no rat occur extremely, with control group ratio, routine blood test, hepatic and renal function, various important organs are all without abnormal change, within the scope of institute's dosage, do not found any toxicity of the present invention.By the acute and chronic toxicity test confirmation of animal, safe range of the present invention is larger, is a kind of safe and reliable health products.

Pharmacodynamic experiment:

By modern Immunology and technology, Study of Traditional Chinese Medicine is to strengthening and regulating the effect of body's immunity, and this has very important meaning to illustrating about the material base of theory of traditional Chinese medical science.The present invention is formed through science compatibility by several natural drugs such as Fructus Corni, Radix Codonopsis, the Radixs Astragali.Existing research discovery, the function of the each system internal organs of Fructus Corni capable of regulating body, all has regulating action to immunocyte and immune molecule.This experiment is intended to further inquire into the molecular immunology mechanism of the present invention to hypoimmunity mouse immune regulating action.

1 materials and methods

1.1 instruments and reagent: Fructus Corni health protection tea of the present invention provides (by embodiment 2 method preparations) by the court; Syklofosfamid ampoule is provided by Hualian Pharmaceutical Co., Ltd., Shanghai.Cytokines measurement kit is all purchased from U.S. R & D company.Flow cytometer, 2000FCA, U.S. company BD production.

1.2 animal Kunming are 30 of male mices, and in 5-7 weeks, body weight is 25 ± 2g, by animal used as test Co., Ltd of Shandong University, is provided.

1.3 method

1.3.1 Animal Model is that male mice is divided into 3 groups at random with grouping by Kunming: blank group, model group and medicine group, 10 every group.Blank group: in experiment process, any processing is not done in normal raising; Model group: intraperitoneal injection 100mg/kg endoxan causes hypoimmunity model, 1 time/d, for three days on end; Medicine group: intraperitoneal injection 100mg/kg endoxan causes hypoimmunity model, 1 time/d, for three days on end, and at the left tibialis anterior of mouse, smear administration at modeling first day, 0.1mL/, booster immunization 1 time after a week, dosage is with for the first time.Collecting sample after 4d.

1.3.2 after the mensuration mouse of mouse immune organ index weighs in, mouse is put to death in cervical vertebra dislocation, get thymus gland, spleen claims its weight in wet base, calculate thymus index and spleen index: heavy × 1000/ body weight 1.3.3 mice serum IgG of heavy × 1000/ body weight spleen index=spleen of thymus index=thymus gland, the mensuration of IgM level is pressed kit description method, measure IgG in mice serum, IgM content.

1.3.4 the T of mouse boosting tissue lymphocyte and subgroup CD4+ thereof, the aseptic spleen of getting of mensuration of CD8+ and CD4+/CD8+, be placed in fill appropriate without Hank ' s liquid plate, gently spleen is ground with tweezers, make single born of the same parents' suspension, through 200 eye mesh screens, filter, cell is suspended in the complete culture solution of 1mL, adjust cell concentration 1 × 10 ⁹l ^-1with the splenocyte suspension that micropipettor fully mixes 100 μ L carefully, be added to the bottom of sample feeding pipe, add 20 μ L monoclonal antibodies, low-speed oscillation mixes 3S, room temperature lucifuge is hatched 30min, after The medicine studyPREP broken red blood cell, up flow type cell instrument detects T cell subsets content.

1.3.5 the mensuration of mice serum IFN-Y, IL-10 level is pressed kit description method, measures the level of IFN-Y, IL-10 in serum.

The all numerical value of 1.2 statistical procedures all represents with x ± s, uses SPSS13.0 statistical software to carry out data analysis, relatively adopts t check between group.Using P<0.05 as statistical significant difference standard.

2 results

2.1 are drawn by table 1 impact of hypoimmunity mouse immune organ, and compared with Normal group, model group Thymus and spleen index significantly reduces, and difference has statistical significance, P<0.01.With model group comparison, medicine group mouse spleen index obviously increases, and has statistical significance, P<0.01, and thymus index also increases to some extent, but difference is not remarkable.

Table 1 the present invention on hypoimmunity mouse immune organ weight impact (n=10, mean ± s)

Group	Index and spleen index (mg/g)	Thymus index (mg/g)
			Normal group	6.06±1.04	3.23±1.01
Model group	2.91±0.89ΔΔ	1.45±0.75ΔΔ
			Of the present invention group	5.28±0.42**	1.85±0.86

With Normal group ratio: Δ: P<0.05, Δ Δ: P<0.01; With model group ratio: *: P<0.05, * *: P<0.01

2.2 are found out by table 2 impact of the T of hypoimmunity mouse boosting tissue lymph subgroup, compared with Normal group, model group mouse spleen CD4+ cell subsets percentage declines, CD8+ cell subsets percentage rises, there is significant difference, P<0.05, CD4+/CD8+ ratio obviously reduces, there is utmost point significant difference, P<0.01.And medicine group can obviously be offset the effect of endoxan destruction immunity, there is significant difference, P<0.05.

Table 2 Buddha's warrior attendant sky shield liquid on hypoimmunity mouse body fluid T cell subsets impact (n=10, mean ± SD)

With Normal group ratio: Δ P<0.05, Δ Δ P<0.05; With than model group: * P<0.05, * * P<0.05.

3 conclusions

The important lymphoid organ that thymus gland, spleen are body.Thymus gland is T cell development, differentiation, and ripe place, its structure and function and fuselage state are closely related.The reduction of thymus index and atrophy of thymus gland are all that mouse is take cellular immunity as main immunosuppressant performance.Spleen is peripheral immune organ maximum in body, and the immunocyte of spleen is take bone-marrow-derived lymphocyte as main, and bone-marrow-derived lymphocyte is the main cell that participates in humoral immunity, and therefore spleen and humoral immunity of organism is in close relations.Thymus index and spleen index have reacted the power of immune body function intuitively.This experiment shows, the present invention has facilitation to the growth of caused by cyclophosphamide hypoimmunity mouse spleen, and the growth of thymus gland is also had to certain facilitation.T lymphocyte can be divided into CD4+ and two subgroups of CD8+ by its function.CD4+T lymphocyte in the immune response of body in very important Central Position, it is by secreting other T of multiple Lymphokine, B cell subsets and participating in immunoreactive other cells, make its propagation and differentiation, and coordinate the interaction between immunocyte, bring into play the function of body immune system.The ratio of CD4+/CD8+ rises, and the just adjusting of prompting immune response is preponderated, and ratio declines, prompting immunologic hypofunction.T lymphocyte subset group's mensuration in human peripheral blood, show that the present invention can make the lymphocytic percentage composition of caused by cyclophosphamide hypoimmunity mouse CD4+T raise, make the ratio of CD4+/CD8+ increase, prompting the present invention plays positive control to T lymphocyte immunity system.IgG, IgM level have reflected humoral immunity ability.This research shows that the low mouse IgG of Fructus Corni health protection tea Promote immunity power, IgM secrete increase.Illustrate that the present invention has not only promoted the enhancing of Immune Function, also has facilitation to humoral immunity simultaneously.

Specific embodiment: go into business certain, man, 43 years old, in first visit on August 9 in 2011.Readme is white collar, and every day, pressure was large at office building, smoked and drink.Often flu of sensation, and insomnia and dreamful sleep recently, easily dry, hypoimmunity.Sexual disorder, can not normal erection.Blood item, B ultrasonic has no organic disease.People in middle age, various pressure and environmental pollution make immune system normally not bring into play protective effect, in the case, very easily cause the infection such as bacterium, virus, fungi, and therefore the most directly to show be exactly liable to illness to hypoimmunity.Because of often ill, increased the weight of the consumption of body, thus generally have a delicate constitution, the performance such as malnutritive, One's spirits are drooping, fatigue and weak, hyposexuality, night pollakiuria, appetite reduction, sleep-disorder, sick, have an injection and take medicine the normal potluck that just gets married.Each sickly all will could recover for a long time, and usually repeatedly outbreak because of take medicine throughout the year, medicine is produced to resentment, suggestion is taken Chinese medicine with the replacement of health tea-drinking.

Side uses: Fructus Corni 30g, root bark of Chinese wolf-berry 12g, bighead atractylodes rhizome 11g, cultivated land 12g, thizoma curculiginis 12g, barrenwort 12g, Radix Codonopsis 14g, Radix Astragali 12g, semen astragali complanati 12g, fruit of Chinese magnoliavine 13g, cortex albiziae 13g, raspberry 12g, Poria cocos 12g, RADIX POLYGONI MULTIFLORI PREPARATA 13g, lucid asparagus 13g, makes tea bag, wrap every day two, takes after 6 days, and patient is conscious, and all diseases are improved, appetite increases, sleep-disorder lowers, One's spirits are drooping improvement, fatigue and weak improve, morning vigorous phenomenon increase, enuresis nocturna does not almost have.Continue to take 1 month, sleep, the state of mind is good greatly, and sexual life harmony.Continue every day and use, all diseases all disappear, full of vitality.

Claims

1. a Fructus Corni health protection tea of improving immunity, is characterized in that, the parts by weight of the various raw materials of described health protection tea comprise: 10～50 parts of Fructus Cornis, 10～20 parts of the root barks of Chinese wolf-berry, 10～20 parts of the bighead atractylodes rhizomes, 10～20 parts, cultivated land, 10～20 parts of thizoma curculiginises, 10～20 parts of barrenwort, 10～20 parts of Radix Codonopsis, 10～20 parts of the Radixs Astragali, 10～20 parts of semen astragali complanatis, 10～20 parts, the fruit of Chinese magnoliavine, 10～20 parts of cortex albiziaes, 10～20 parts of raspberries, 10～20 parts, Poria cocos, 10～20 parts of RADIX POLYGONI MULTIFLORI PREPARATAs, 10～20 parts of lucid asparagus.

2. improve according to claim 1 the Fructus Corni health protection tea of immunity, it is characterized in that, the parts by weight of the various raw materials of described smart health protection tea are: 10～30 parts of Fructus Cornis, 10～15 parts of the root barks of Chinese wolf-berry, 10～15 parts of the bighead atractylodes rhizomes, 10～15 parts, cultivated land, 10～15 parts of thizoma curculiginises, 10～15 parts of barrenwort, 10～15 parts of Radix Codonopsis, 10～20 parts of the Radixs Astragali, 10～20 parts of semen astragali complanatis, 10～20 parts, the fruit of Chinese magnoliavine, 10～20 parts of cortex albiziaes, 10～20 parts of raspberries, 10～20 parts, Poria cocos, 10～20 parts of RADIX POLYGONI MULTIFLORI PREPARATAs, 10～20 parts of lucid asparagus.

3. improve according to claim 1 the Fructus Corni health protection tea of immunity, it is characterized in that, the parts by weight of the various raw materials of described health protection tea are: 10～40 parts of Fructus Cornis, 10～20 parts of the root barks of Chinese wolf-berry, 10～20 parts of the bighead atractylodes rhizomes, 10～20 parts, cultivated land, 10～20 parts of thizoma curculiginises, 10～20 parts of barrenwort, 10～20 parts of Radix Codonopsis, 10～15 parts of the Radixs Astragali, 10～15 parts of semen astragali complanatis, 10～15 parts, the fruit of Chinese magnoliavine, 10～15 parts of cortex albiziaes, 10～15 parts of raspberries, 10～15 parts, Poria cocos, 10～15 parts of RADIX POLYGONI MULTIFLORI PREPARATAs, 10～15 parts of lucid asparagus.

4. a preparation method who improves the Fructus Corni health protection tea of immunity as described in claim 1～3 any one, is characterized in that, the preparation process of described Fructus Corni health protection tea comprises following aspect:

A. the preparation of Fructus Corni;

5. improve according to claim 4 the preparation method of the Fructus Corni health protection tea of immunity, it is characterized in that, in described step a, picking fruit when the time of the year when autumn changes into winter, Fructus Corni pericarp reddened, after drying or put in boiling water and slightly to scald with slow fire, in time except removal core, dry, dry raw dogwood fruit and put a pot interior water proof steam 30-60 minute at 80-100 ℃, steam to puce, then dry.

6. the preparation method who improves according to claim 4 the Fructus Corni health protection tea of immunity, is characterized in that, in described step b, surplus stock is cleaned to impurity elimination, steeps into ethanol, and heating and refluxing extraction 2 times, each 1～2 hour, merges standing by No. 2 extracts; Above-mentioned two kinds of extracts are merged, and decompression recycling ethanol to be concentrated into liquor strength be 0.6g crude drug/mL, after suction filtration, when the relative density of filtrate is about 20 ℃ 1.08; Be decompressed to 0.03-0.08MPa, temperature remains on 60-80 ℃, and being concentrated into relative density is 1.20, the medicinal extract that temperature to 60 is ℃-70 ℃.

7. improve according to claim 4 the preparation method of the Fructus Corni health protection tea of immunity, it is characterized in that, in described step c, the medicinal extract that above-mentioned steps is extracted is inserted in economic benefits and social benefits vacuum concentrator, the concentrate that while being concentrated into 90 ℃, relative density is 1.05, set to 0～5 ℃ of deepfreezes 24 hours; Refrigeration liquid is added to 0.3% filter aid diatomite, filter, filtrate is inserted in economic benefits and social benefits vacuum concentrator again, is concentrated into every 1ml containing 1g crude drug amount, then adds dextrin and puts into granulator and granulate.

8. the preparation method who improves according to claim 4 the Fructus Corni health protection tea of immunity, is characterized in that, in described steps d, the particle that the Fructus Corni preparing is prepared with other raw materials mixes, and ultraviolet disinfection is packed and be get final product.

9. a basis improves the preparation method of the Fructus Corni health protection tea of immunity as described in claim 1～3 any one, it is characterized in that, when other raw materials of described raising immunity Fructus Corni essence health protection tea are made pulvis, step is: other raw materials are put into 5-10 times of amount ethanol, soak 1-2 hours, heating is extracted 2 times, each 1-2 hours, remove supernatant, merge extract, 100-120 orders filter, through the molecular weight that dams, be 5000-10000 ultrafiltration post ultrafiltration again, 1.36 medicinal extract when ultrafiltrate reduced pressure concentration relative density is 80 ℃, then put into drying baker and be dried into powder, after ultraviolet radiation for sterilizing and disinfecting, merge packing with the Fructus Corni preparing.

10. a basis improves the preparation method of the Fructus Corni health protection tea of immunity as described in claim 1～3 any one, it is characterized in that, when other raw materials of described raising immunity Fructus Corni health protection tea are made paste, step is: other raw materials are put into 5-10 times of amount ethanol, soak 1-2 hours, heating is extracted 2 times, each 1-2 hours, removes supernatant, merge extract, 100-120 orders filter; The dregs of a decoction of other bulk drugs of more above-mentioned alcohol extract being crossed add 10 times of water gaging heating and refluxing extraction 2 times, each 1～2 hour, No. 2 extracts are merged standing; Above-mentioned two kinds of extracts are merged, and decompression recycling ethanol, and to be concentrated into liquor strength be 0.6g crude drug/mL, after suction filtration, when the relative density of filtrate is about 20 ℃ 1.08; Be decompressed to 0.03-0.08MPa, temperature remains on 60-80 ℃, and being concentrated into relative density is 1.20, the medicinal extract that temperature to 60 is ℃-70 ℃.