CN103733975A - Method suitable for rapid rooting of blueberry tissue culture seedlings - Google Patents
Method suitable for rapid rooting of blueberry tissue culture seedlings Download PDFInfo
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- CN103733975A CN103733975A CN201410001429.2A CN201410001429A CN103733975A CN 103733975 A CN103733975 A CN 103733975A CN 201410001429 A CN201410001429 A CN 201410001429A CN 103733975 A CN103733975 A CN 103733975A
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- cultivation cup
- culturing rack
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
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- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Abstract
The invention discloses a method suitable for rapid rooting of blueberry tissue culture seedlings. Three culture frames which are connected in series one above the other are used, and corresponding most suitable culture solutions are used at different stages and flow on a longer path, thereby increasing the yield and culture speed of the culture seedlings, and saving the using quantities of the culture solutions. In the method, a culture solution ingredient sensor is also used.
Description
Technical field
The invention belongs to field of plant tissue culture technique, be specifically related to a kind of method of suitable blueberry tissue culture seedling Rapid Rooting.
Background technology
The cultivation cycle of blueberry often can not meet the needs of time compared with length.The cultivation speed of cultivating seedling in order to improve blueberry, can adopt the method for using different culture fluids in different phase.And cultivate in the cultivating process of seedling at large batch of blueberry, need to expend quite a large amount of culture fluids.And in prior art, also do not have to cultivate seedling, the scheme that can save again culture fluid by fast culture blueberry.
Summary of the invention
In order to solve the problems of the technologies described above, the invention provides a kind of method of suitable blueberry tissue culture seedling Rapid Rooting, it can obviously improve the grown cultures speed of blueberry, can save the utilization of culture fluid again simultaneously.
The scheme that the present invention adopts is:
A kind of method of suitable blueberry tissue culture seedling Rapid Rooting, it uses a kind of blueberry tissue culture seedling Rapid Rooting system, described system comprises: the first culturing rack, the second culturing rack, the 3rd culturing rack and culture fluid allocation units, described the first culturing rack, the second culturing rack and the 3rd culturing rack are arranged successively up and down, described culture fluid allocation units are connected with the cultivation cup in three culturing racks by main line, wherein, cultivation cup in described the first culturing rack is connected with main line by the first valve, cultivation cup in described the second culturing rack is connected with main line by the second valve, cultivation cup in described the 3rd culturing rack is connected with main line by the 3rd valve,
Wherein, in described the first culturing rack, there is the first left cultivation cup and the first right cultivation cup, described the first left cultivation cup is connected with described the first valve by the first left valve, described the first right cultivation cup is connected with described the first valve by the first right valve, described the first left bottom of cultivating cup is connected with the first left discharge pipe by the first left dump valve, in described the first left cultivation cup, is also provided with the first left culture fluid component sensor; Described the first right bottom of cultivating cup is connected with the first right discharge pipe by the first right dump valve, in described the first right cultivation cup, is also provided with the first right culture fluid component sensor;
In described the second culturing rack, there is the second left cultivation cup and the second right cultivation cup, described the second left cultivation cup is connected with described the second valve by the second left valve, described the second right cultivation cup is connected with described the second valve by the second right valve, described the second left bottom of cultivating cup is connected with the second left discharge pipe by the second left dump valve, in described the second left cultivation cup, is also provided with the second left culture fluid component sensor; Described the second right bottom of cultivating cup is connected with the second right discharge pipe by the second right dump valve, in described the second right cultivation cup, is also provided with the second right culture fluid component sensor;
In described the 3rd culturing rack, there is the 3rd left cultivation cup and the 3rd right cultivation cup, described the 3rd left cultivation cup is connected with described the 3rd valve by the 3rd left valve, described the 3rd right cultivation cup is connected with described the 3rd valve by the 3rd right valve, described the 3rd left bottom of cultivating cup is connected with the 3rd left discharge pipe by the 3rd left dump valve, in described the 3rd left cultivation cup, is also provided with the 3rd left culture fluid component sensor; Described the 3rd right bottom of cultivating cup is connected with the 3rd right discharge pipe by the 3rd right dump valve, in described the 3rd right cultivation cup, is also provided with the 3rd right culture fluid component sensor;
Described the first left discharge pipe is connected with described the second left cultivation cup, and described the second left discharge pipe is connected with described the 3rd left cultivation cup, and described the first right discharge pipe is connected with described the second right cultivation cup, and described the second right discharge pipe is connected with described the 3rd right cultivation cup;
Described method comprises the steps:
1) first is cultivated to the cultivation cup that seedling is put into described the first culturing rack;
2) by culture fluid allocation units, in the cultivation cup of described the first culturing rack, inject the first culture fluid;
3), after suitable culture period, the first culture fluid in the cultivation cup of described the first culturing rack is discharged in the cultivation cup in described the second culturing rack;
4) by described culture fluid allocation units, in the cultivation cup of the first culturing rack, inject the second culture fluid;
5) second batch is cultivated to the cultivation cup that seedling is put into described the second culturing rack;
6), after suitable culture period, the first culture fluid in the cultivation cup of described the second culturing rack is discharged in the cultivation cup in described the 3rd culturing rack;
7) the second culture fluid in the cultivation cup of described the first culturing rack is discharged in the cultivation cup in described the second culturing rack;
8) by culture fluid allocation units, in the cultivation cup of described the first culturing rack, inject the 3rd culture fluid;
9) by the 3rd batch, cultivate the cultivation cup that seedling is put into described the 3rd culturing rack;
10), after suitable culture period, the first culture fluid in the cultivation cup in described the 3rd culturing rack is discharged;
11) the second culture fluid in the cultivation cup of described the second culturing rack is discharged in the cultivation cup in described the 3rd culturing rack;
12) the 3rd culture fluid in the cultivation cup of described the first culturing rack is discharged in the cultivation cup in described the second culturing rack; First is cultivated seedling and shifts out cultivation cup;
13), after suitable culture period, the 3rd culture fluid in the cultivation cup of described the second culturing rack is discharged in the cultivation cup in described the 3rd culturing rack; Second batch is cultivated seedling and is shifted out cultivation cup;
14), after suitable culture period, the 3rd culture fluid in the cultivation cup of described the 3rd culturing rack is discharged; Cultivate seedling for the 3rd batch and shift out cultivation cup;
In incubation, by the composition of culture fluid component sensor monitoring culture fluid, when the composition of culture fluid does not meet the demands, by described culture fluid allocation units, in corresponding cultivation cup, inject corresponding culture fluid.
By such scheme, owing to having used corresponding the most suitable culture fluid in the different stages, therefore improved the cultivation speed that blueberry is cultivated seedling; Meanwhile, three culturing racks have in fact also increased the cultivation quantity that blueberry is cultivated seedling; And three culturing racks are connected up and down, in fact make the culture fluid use of trickling on longer path, make its use more abundant, saved the consumption of culture fluid; Be equipped with culture fluid component sensor simultaneously, the composition quality of culture fluid is guaranteed.
Accompanying drawing explanation
Fig. 1 is the structural representation of cultural method of the present invention.
Embodiment
With reference to Fig. 1, a kind of method of suitable blueberry tissue culture seedling Rapid Rooting, it uses a kind of blueberry tissue culture seedling Rapid Rooting system, described system comprises: the first culturing rack 1, the second culturing rack 2, the 3rd culturing rack 3 and culture fluid allocation units 10, described the first culturing rack 1, the second culturing rack 2 and the 3rd culturing rack 3 are arranged successively up and down, described culture fluid allocation units 10 are connected with the cultivation cup in three culturing racks by main line 11, wherein, cultivation cup in described the first culturing rack 1 is connected with main line 11 by the first valve 12, cultivation cup in described the second culturing rack 2 is connected with main line 11 by the second valve 13, cultivation cup in described the 3rd culturing rack 3 is connected with main line 11 by the 3rd valve 14,
Wherein, in described the first culturing rack 1, there is the right cultivation cup 4 of the first left cultivation cup 5 and first, described the first left cultivation cup 5 is connected with described the first valve 12 by the first left valve 51, described the first right cultivation cup 4 is connected with described the first valve 12 by the first right valve 41, described the first left bottom of cultivating cup 5 is connected with the first left discharge pipe 54 by the first left dump valve 52, in described the first left cultivation cup 5, is also provided with the first left culture fluid component sensor 53; Described the first right bottom of cultivating cup 4 is connected with the first right discharge pipe 44 by the first right dump valve 42, in described the first right cultivation cup 4, is also provided with the first right culture fluid component sensor 43;
In described the second culturing rack 2, there is the right cultivation cup 7 of the second left cultivation cup 6 and second, described the second left cultivation cup 6 is connected with described the second valve 13 by the second left valve 61, described the second right cultivation cup 7 is connected with described the second valve 13 by the second right valve 71, described the second left bottom of cultivating cup 6 is connected with the second left discharge pipe 64 by the second left dump valve 62, in described the second left cultivation cup 6, is also provided with the second left culture fluid component sensor 63; Described the second right bottom of cultivating cup 7 is connected with the second right discharge pipe 74 by the second right dump valve 72, in described the second right cultivation cup 7, is also provided with the second right culture fluid component sensor 73;
In described the 3rd culturing rack 3, there is the right cultivation cup 9 of the 3rd left cultivation cup the 8 and the 3rd, described the 3rd left cultivation cup 8 is connected with described the 3rd valve 14 by the 3rd left valve 81, described the 3rd right cultivation cup 9 is connected with described the 3rd valve 14 by the 3rd right valve 91, described the 3rd left bottom of cultivating cup 8 is connected with the 3rd left discharge pipe 84 by the 3rd left dump valve 82, in described the 3rd left cultivation cup 8, is also provided with the 3rd left culture fluid component sensor 83; Described the 3rd right bottom of cultivating cup 9 is connected with the 3rd right discharge pipe 94 by the 3rd right dump valve 92, in described the 3rd right cultivation cup 9, is also provided with the 3rd right culture fluid component sensor 93;
Described the first left discharge pipe 54 is connected with described the second left cultivation cup 6, described the second left discharge pipe 64 is connected with described the 3rd left cultivation cup 8, described the first right discharge pipe 44 is connected with described the second right cultivation cup 7, and described the second right discharge pipe 74 is connected with described the 3rd right cultivation cup 9;
Described method comprises the steps:
1) first is cultivated to the cultivation cup that seedling is put into described the first culturing rack 1;
2) by culture fluid allocation units 10, in the cultivation cup of described the first culturing rack 1, inject the first culture fluid;
3), after suitable culture period, the first culture fluid in the cultivation cup of described the first culturing rack 1 is discharged in the cultivation cup in described the second culturing rack 2;
4) by described culture fluid allocation units 10, in the cultivation cup of the first culturing rack 1, inject the second culture fluid;
5) second batch is cultivated to the cultivation cup that seedling is put into described the second culturing rack 2;
6), after suitable culture period, the first culture fluid in the cultivation cup of described the second culturing rack 2 is discharged in the cultivation cup in described the 3rd culturing rack 3;
7) the second culture fluid in the cultivation cup of described the first culturing rack 1 is discharged in the cultivation cup in described the second culturing rack 2;
8) by culture fluid allocation units 10, in the cultivation cup of described the first culturing rack 1, inject the 3rd culture fluid;
9) by the 3rd batch, cultivate the cultivation cup that seedling is put into described the 3rd culturing rack;
10), after suitable culture period, the first culture fluid in the cultivation cup in described the 3rd culturing rack is discharged;
11) the second culture fluid in the cultivation cup of described the second culturing rack 2 is discharged in the cultivation cup in described the 3rd culturing rack 3;
12) the 3rd culture fluid in the cultivation cup of described the first culturing rack 1 is discharged in the cultivation cup in described the second culturing rack 2; First is cultivated seedling and shifts out cultivation cup;
13), after suitable culture period, the 3rd culture fluid in the cultivation cup of described the second culturing rack 2 is discharged in the cultivation cup in described the 3rd culturing rack 3; Second batch is cultivated seedling and is shifted out cultivation cup;
14), after suitable culture period, the 3rd culture fluid in the cultivation cup of described the 3rd culturing rack 3 is discharged; Cultivate seedling for the 3rd batch and shift out cultivation cup;
In incubation, by the composition of culture fluid component sensor monitoring culture fluid, when the composition of culture fluid does not meet the demands, by described culture fluid allocation units 10, in corresponding cultivation cup, inject corresponding culture fluid.
Claims (1)
1. the method for a suitable blueberry tissue culture seedling Rapid Rooting, it uses a kind of blueberry tissue culture seedling Rapid Rooting system, described system comprises: the first culturing rack (1), the second culturing rack (2), the 3rd culturing rack (3) and culture fluid allocation units (10), described the first culturing rack (1), the second culturing rack (2) and the 3rd culturing rack (3) are arranged successively up and down, described culture fluid allocation units (10) are connected with the cultivation cup in three culturing racks by main line (11), wherein, cultivation cup in described the first culturing rack (1) is connected with main line (11) by the first valve (12), cultivation cup in described the second culturing rack (2) is connected with main line (11) by the second valve (13), cultivation cup in described the 3rd culturing rack (3) is connected with main line (11) by the 3rd valve (14),
Wherein, in described the first culturing rack (1), there is the first left cultivation cup (5) and the first right cultivation cup (4), described the first left cultivation cup (5) is connected with described the first valve (12) by the first left valve (51), described the first right cultivation cup (4) is connected with described the first valve (12) by the first right valve (41), described the first left bottom of cultivating cup (5) is connected with the first left discharge pipe (54) by the first left dump valve (52), in described the first left cultivation cup (5), is also provided with the first left culture fluid component sensor (53); Described the first right bottom of cultivating cup (4) is connected with the first right discharge pipe (44) by the first right dump valve (42), in described the first right cultivation cup (4), is also provided with the first right culture fluid component sensor (43);
In described the second culturing rack (2), there is the second left cultivation cup (6) and the second right cultivation cup (7), described the second left cultivation cup (6) is connected with described the second valve (13) by the second left valve (61), described the second right cultivation cup (7) is connected with described the second valve (13) by the second right valve (71), described the second left bottom of cultivating cup (6) is connected with the second left discharge pipe (64) by the second left dump valve (62), in described the second left cultivation cup (6), is also provided with the second left culture fluid component sensor (63); Described the second right bottom of cultivating cup (7) is connected with the second right discharge pipe (74) by the second right dump valve (72), in described the second right cultivation cup (7), is also provided with the second right culture fluid component sensor (73);
In described the 3rd culturing rack (3), there is the 3rd left cultivation cup (8) and the 3rd right cultivation cup (9), described the 3rd left cultivation cup (8) is connected with described the 3rd valve (14) by the 3rd left valve (81), described the 3rd right cultivation cup (9) is connected with described the 3rd valve (14) by the 3rd right valve (91), described the 3rd left bottom of cultivating cup (8) is connected with the 3rd left discharge pipe (84) by the 3rd left dump valve (82), in described the 3rd left cultivation cup (8), is also provided with the 3rd left culture fluid component sensor (83); Described the 3rd right bottom of cultivating cup (9) is connected with the 3rd right discharge pipe (94) by the 3rd right dump valve (92), in described the 3rd right cultivation cup (9), is also provided with the 3rd right culture fluid component sensor (93);
Described the first left discharge pipe (54) is connected with described the second left cultivation cup (6), described the second left discharge pipe (64) is connected with described the 3rd left cultivation cup (8), described the first right discharge pipe (44) is connected with described the second right cultivation cup (7), and described the second right discharge pipe (74) is connected with described the 3rd right cultivation cup (9);
Described method comprises the steps:
1) first is cultivated to the cultivation cup that seedling is put into described the first culturing rack (1);
2) by culture fluid allocation units (10), in the cultivation cup of described the first culturing rack (1), inject the first culture fluid;
3), after suitable culture period, the first culture fluid in the cultivation cup of described the first culturing rack (1) is discharged in the cultivation cup in described the second culturing rack (2);
4) by described culture fluid allocation units (10), in the cultivation cup of the first culturing rack (1), inject the second culture fluid;
5) second batch is cultivated to the cultivation cup that seedling is put into described the second culturing rack (2);
6), after suitable culture period, the first culture fluid in the cultivation cup of described the second culturing rack (2) is discharged in the cultivation cup in described the 3rd culturing rack (3);
7) the second culture fluid in the cultivation cup of described the first culturing rack (1) is discharged in the cultivation cup in described the second culturing rack (2);
8) by culture fluid allocation units (10), in the cultivation cup of described the first culturing rack (1), inject the 3rd culture fluid;
9) by the 3rd batch, cultivate the cultivation cup that seedling is put into described the 3rd culturing rack;
10), after suitable culture period, the first culture fluid in the cultivation cup in described the 3rd culturing rack is discharged;
11) the second culture fluid in the cultivation cup of described the second culturing rack (2) is discharged in the cultivation cup in described the 3rd culturing rack (3);
12) the 3rd culture fluid in the cultivation cup of described the first culturing rack (1) is discharged in the cultivation cup in described the second culturing rack (2); First is cultivated seedling and shifts out cultivation cup;
13), after suitable culture period, the 3rd culture fluid in the cultivation cup of described the second culturing rack (2) is discharged in the cultivation cup in described the 3rd culturing rack (3); Second batch is cultivated seedling and is shifted out cultivation cup;
14), after suitable culture period, the 3rd culture fluid in the cultivation cup of described the 3rd culturing rack (3) is discharged; Cultivate seedling for the 3rd batch and shift out cultivation cup;
In incubation, by the composition of culture fluid component sensor monitoring culture fluid, when the composition of culture fluid does not meet the demands, by described culture fluid allocation units (10), in corresponding cultivation cup, inject corresponding culture fluid.
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| CN201410001429.2A CN103733975B (en) | 2014-01-03 | 2014-01-03 | Method suitable for rapid rooting of blueberry tissue culture seedlings |
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| CN201410001429.2A CN103733975B (en) | 2014-01-03 | 2014-01-03 | Method suitable for rapid rooting of blueberry tissue culture seedlings |
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| CN103733975B CN103733975B (en) | 2015-02-04 |
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US4729188A (en) * | 1986-12-09 | 1988-03-08 | Cheng Ming Kwei | Automatic sprouts culture bag |
| JPS6474936A (en) * | 1987-09-14 | 1989-03-20 | Sumitomo Cement Co | Culture through pebble by multi-stage bed and device therefor |
| CN1143893A (en) * | 1994-03-11 | 1997-02-26 | 株式会社诚和 | Multistage plant culture method and multistage plant culture apparatus for use therein |
| JP2002101757A (en) * | 2000-09-27 | 2002-04-09 | Hokuriku Electric Power Co Inc:The | Method for culturing plant and plant culture device used for the method |
| JP2006280252A (en) * | 2005-03-31 | 2006-10-19 | Koken Boring Mach Co Ltd | Underground agricultural factory system |
| CN201230501Y (en) * | 2008-07-17 | 2009-05-06 | 蒲志理 | Water-ploughing circulation cultivation system |
| CN201682856U (en) * | 2010-02-24 | 2010-12-29 | 丽莱登实业有限公司 | Base frame device for water plow homework |
| CN102577921A (en) * | 2011-01-10 | 2012-07-18 | 太仓戈林农业科技有限公司 | Soilless culture device |
-
2014
- 2014-01-03 CN CN201410001429.2A patent/CN103733975B/en not_active Expired - Fee Related
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4729188A (en) * | 1986-12-09 | 1988-03-08 | Cheng Ming Kwei | Automatic sprouts culture bag |
| JPS6474936A (en) * | 1987-09-14 | 1989-03-20 | Sumitomo Cement Co | Culture through pebble by multi-stage bed and device therefor |
| CN1143893A (en) * | 1994-03-11 | 1997-02-26 | 株式会社诚和 | Multistage plant culture method and multistage plant culture apparatus for use therein |
| JP2002101757A (en) * | 2000-09-27 | 2002-04-09 | Hokuriku Electric Power Co Inc:The | Method for culturing plant and plant culture device used for the method |
| JP2006280252A (en) * | 2005-03-31 | 2006-10-19 | Koken Boring Mach Co Ltd | Underground agricultural factory system |
| CN201230501Y (en) * | 2008-07-17 | 2009-05-06 | 蒲志理 | Water-ploughing circulation cultivation system |
| CN201682856U (en) * | 2010-02-24 | 2010-12-29 | 丽莱登实业有限公司 | Base frame device for water plow homework |
| CN102577921A (en) * | 2011-01-10 | 2012-07-18 | 太仓戈林农业科技有限公司 | Soilless culture device |
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