CN103704109B - Fast rooting method for blueberry tissue culture seedlings outside bottles and rooting culture medium - Google Patents
Fast rooting method for blueberry tissue culture seedlings outside bottles and rooting culture medium Download PDFInfo
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- CN103704109B CN103704109B CN201410001446.6A CN201410001446A CN103704109B CN 103704109 B CN103704109 B CN 103704109B CN 201410001446 A CN201410001446 A CN 201410001446A CN 103704109 B CN103704109 B CN 103704109B
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- nutrient chemical
- dividing plate
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- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
Abstract
Provided are a fast rooting method for blueberry tissue culture seedlings out bottles and a rooting culture medium. An outside-bottle seedling culture tray system is used, and the outside-bottle seedling culture tray system comprises a seedling culture tray, a culture agent supply assembly and a supply pipeline assembly. Two supply pipelines are respectively provided with a front liquid discharge nozzle and a rear liquid discharge nozzle in every seedling culture unit lattice, and the front liquid discharge nozzles and the rear liquid discharge nozzles incline downwards and face the inner sides of the corresponding unit lattices to discharge liquid. The culture agent supply assembly comprises a first culture agent supply device, a second culture agent supply device and a third culture agent supply device.
Description
Technical field
The invention belongs to field of plant tissue culture technique, be specifically related to a kind of quick outside sprout-cultivating-bottle method and culture of rootage matrix of blueberry tissue culture seedling.
Background technology
The cultivation cycle of blueberry is often longer and can not meet the needs of time.Cultivating the cultivation speed of seedling in order to improve blueberry, the method using different nutrient chemical in different phase can be adopted.And due to matrix when blueberry seedling cultivated outward by bottle be solid, and the root system dynamic growth of blueberry in matrix, is therefore difficult to the corresponding site be supplied to targetedly by the nutrient chemical of needs in matrix.
Summary of the invention
In order to solve the problems of the technologies described above, the invention provides a kind of quick outside sprout-cultivating-bottle method of the blueberry tissue culture seedling with inclination liquid outlet, nutrient chemical can be provided in culture matrix by targetedly, can significantly improve the grown cultures speed of blueberry.
According to an aspect of the present invention, the scheme that the present invention adopts is:
A quick outside sprout-cultivating-bottle method for blueberry tissue culture seedling, it uses a kind of bottle to train seedling disc system outward, and described bottle is trained seedling disc system outward and comprised: training seedling dish, nutrient chemical feeding assembly and feeding pipe assembly.
Training seedling dish comprises outside framework, outside framework is the cuboid of opened top, the inside of outside framework from top to bottom successively level be fixed with first can sepage dividing plate, second can sepage dividing plate and the 3rd can sepage dividing plate, thus form three layers of seedbed frame in the inside of described outside framework, describedly can to make by degradation material by sepage dividing plate, the inside of outside framework can the below of sepage dividing plate be liquid collecting chamber the 3rd, the bottom righthand side of described liquid collecting chamber is provided with liquid collecting chamber liquid outlet, the inner vertical of outside framework is provided with multiple vertical dividing plate, vertical dividing plate from the At The Height of the end face of the cuboid of described outside framework through first can sepage dividing plate and second can sepage dividing plate extend to the 3rd can sepage dividing plate, thus the inner space of outside framework is divided into multiple training seedling cell, the low order end of the inner space of described outside framework is provided with discharge opeing lattice, the first liquid-impermeable dividing plate is disposed with from top to bottom in described discharge opeing lattice, second liquid-impermeable dividing plate and the 3rd liquid-impermeable dividing plate, the left part of described three liquid-impermeable dividing plates is from left to right downward-sloping, right part level, the left part of described three liquid-impermeable dividing plates respectively with described three can the right part termination of sepage dividing plate, the right part of described three liquid-impermeable dividing plates is all fixedly connected with outside framework, described discharge opeing lattice are divided into three the discharge opeing compartments corresponding with described three layers of seedbed frame by described three liquid-impermeable dividing plates, leakage fluid dram is provided with on the right side of each discharge opeing compartment.
Every layer of three layers of seedbed frame can be provided with nutrient chemical supply pipe group above sepage dividing plate accordingly, the quantity of described nutrient chemical supply pipe group is three, described nutrient chemical supply pipe group in every layer extends each training seedling cell penetrated in the layer of place, described nutrient chemical supply pipe group has two supply pipelines, described two supply pipelines are separately positioned on front side wall and the rear wall place of respective cells, and described two supply pipelines are liquid outlet and rear liquid outlet before each training seedling cell place is respectively arranged with, described front liquid outlet and rear liquid outlet are all inclined downward towards the inner side fluid of respective cells.
Nutrient chemical feeding assembly comprises the first nutrient chemical feedway, second nutrient chemical feedway and the 3rd nutrient chemical feedway, described first nutrient chemical feedway, second nutrient chemical feedway is connected with feeding pipe assembly respectively by three supply valves with the 3rd nutrient chemical feedway, feeding pipe assembly is connected with three described nutrient chemical supply pipe groups respectively by three receiving valves, described nutrient chemical feeding assembly is also provided with nutrient chemical supplementary device, described nutrient chemical supplementary device is respectively by three replenishment valve and the first nutrient chemical feedway, second nutrient chemical feedway is connected with the 3rd nutrient chemical feedway.
The step of described method is:
1) corresponding culture matrix is respectively charged at every layer of three layers of seedbed frame;
2) blueberry seedling is inserted in each training seedling cell, make the end of blueberry seedling can sepage dividing plate place first;
3) in the nursery first stage, control corresponding valve, making the first nutrient chemical in the first nutrient chemical feedway be supplied to first can in the training seedling cell above sepage dividing plate;
4) in nursery second stage, control corresponding valve, making the second nutrient chemical in the second nutrient chemical feedway be supplied to second can in the training seedling cell above sepage dividing plate;
5) in the phase III of nursery, control corresponding valve, making the 3rd nutrient chemical in the 3rd nutrient chemical feedway be supplied to the 3rd can in the training seedling cell above sepage dividing plate.
When nutrient chemical in every layer of three layers of seedbed frame spills in described discharge opeing lattice, nutrient chemical is discharged by corresponding leakage fluid dram; When nutrient chemical penetrates in liquid collecting chamber, nutrient chemical is discharged by liquid collecting chamber liquid outlet.
According to a further aspect in the invention, according to the quick outside sprout-cultivating-bottle method of the above-mentioned blueberry tissue culture seedling with inclination liquid outlet and obtained culture matrix.
By such scheme, owing to employing corresponding nutrient chemical the most suitable in the different stages, therefore improve the cultivation speed that blueberry cultivates seedling; Meanwhile, employ different culture matrixes in different phase, also meet the needs of blueberry seedling Different growth phases; Meanwhile, unnecessary nutrient chemical can be discharged in time by the leakage fluid dram of right side and below, and the consumption of nutrient chemical can be made moderate, prevents blueberry bad root phenomenon from occurring.And corresponding nutrient chemical also can be input to the position of corresponding blueberry seedling by the feed mechanism of nutrient chemical targetedly, facilitate the quick training seedling of blueberry seedling.
Accompanying drawing explanation
Fig. 1 is training seedling disc system structural representation of the present invention.
Fig. 2 is the liquid outlet structure schematic diagram of nutrient chemical supply pipe group of the present invention.
Embodiment
With reference to Fig. 1-2, a kind of quick outside sprout-cultivating-bottle method of blueberry tissue culture seedling, it uses a kind of bottle to train seedling disc system outward, and described bottle is trained seedling disc system outward and comprised: training seedling dish, nutrient chemical feeding assembly and feeding pipe assembly 16.
Training seedling dish 12 comprises outside framework 12, outside framework 12 is the cuboid of opened top, the inside of outside framework 12 from top to bottom successively level be fixed with first can sepage dividing plate 6, second can sepage dividing plate 7 and the 3rd can sepage dividing plate 8, thus form three layers of seedbed frame in the inside of described outside framework 12, describedly can to make by degradation material by sepage dividing plate, the inside of outside framework 12 can the below of sepage dividing plate 8 be liquid collecting chamber 17 the 3rd, the bottom righthand side of described liquid collecting chamber 17 is provided with liquid collecting chamber liquid outlet 14, the inner vertical of outside framework 12 is provided with multiple vertical dividing plate 2, vertical dividing plate 2 from the At The Height of the end face of the cuboid of described outside framework 12 through first can sepage dividing plate 6 and second can sepage dividing plate 7 extend to the 3rd can sepage dividing plate 8, thus the inner space of outside framework 12 is divided into multiple training seedling cell 1, the low order end of the inner space of described outside framework 12 is provided with discharge opeing lattice 15, the first liquid-impermeable dividing plate 9 is disposed with from top to bottom in described discharge opeing lattice 15, second liquid-impermeable dividing plate 10 and the 3rd liquid-impermeable dividing plate 11, described three liquid-impermeable dividing plates 9, 10, the left part of 11 is from left to right downward-sloping, right part level, the left part of described three liquid-impermeable dividing plates 9 respectively with described three can sepage dividing plate 6, 7, the right part termination of 8, described three liquid-impermeable dividing plates 9, 10, the right part of 11 is all fixedly connected with outside framework 12, described three liquid-impermeable dividing plates 9, 10, described discharge opeing lattice 15 are divided into three the discharge opeing compartments corresponding with described three layers of seedbed frame by 11, leakage fluid dram 13 is provided with on the right side of each discharge opeing compartment.
Every layer of three layers of seedbed frame accordingly can sepage dividing plate 6, 7, nutrient chemical supply pipe group 3 is provided with above in the of 8, the quantity of described nutrient chemical supply pipe group 3 is three, described nutrient chemical supply pipe group 3 in every layer extends each training seedling cell 1 penetrated in the layer of place, described nutrient chemical supply pipe group 3 has two supply pipelines, described two supply pipelines are separately positioned on front side wall and the rear wall place of respective cells, and described two supply pipelines are liquid outlet 31 and rear liquid outlet 32 before each training seedling cell 1 place is respectively arranged with, described front liquid outlet 31 and rear liquid outlet 32 are all inclined downward towards the inner side fluid of respective cells.
Nutrient chemical feeding assembly comprises the first nutrient chemical feedway 22, second nutrient chemical feedway 23 and the 3rd nutrient chemical feedway 24, described first nutrient chemical feedway 22, second nutrient chemical feedway 23 is connected with feeding pipe assembly 16 respectively by three supply valves with the 3rd nutrient chemical feedway 24, feeding pipe assembly 16 is connected with three described nutrient chemical supply pipe groups 3 respectively by three receiving valves, described nutrient chemical feeding assembly is also provided with nutrient chemical supplementary device 25, described nutrient chemical supplementary device 25 is respectively by three replenishment valve and the first nutrient chemical feedway 22, second nutrient chemical feedway 23 is connected with the 3rd nutrient chemical feedway 24.
The step of described method is:
1) corresponding culture matrix is respectively charged at every layer of three layers of seedbed frame;
2) blueberry seedling is inserted in each training seedling cell 1, make the end of blueberry seedling can sepage dividing plate 6 place first;
3) in the nursery first stage, control corresponding valve, making the first nutrient chemical in the first nutrient chemical feedway 22 be supplied to first can in the training seedling cell 1 above sepage dividing plate 6;
4) in nursery second stage, control corresponding valve, making the second nutrient chemical in the second nutrient chemical feedway 23 be supplied to second can in the training seedling cell 1 above sepage dividing plate 7;
5) in the phase III of nursery, control corresponding valve, making the 3rd nutrient chemical in the 3rd nutrient chemical feedway 24 be supplied to the 3rd can in the training seedling cell 1 above sepage dividing plate 8.
When nutrient chemical in every layer of three layers of seedbed frame spills in described discharge opeing lattice 15, nutrient chemical is discharged by corresponding leakage fluid dram 13; When nutrient chemical penetrates in liquid collecting chamber 17, nutrient chemical is discharged by liquid collecting chamber liquid outlet 14.
Three layers of matrix can comprise sand, dry liver moss, peat soil etc.
Claims (2)
1. a quick outside sprout-cultivating-bottle method for blueberry tissue culture seedling, it uses a kind of bottle to train seedling disc system outward, and described bottle is trained seedling disc system outward and comprised: training seedling dish, nutrient chemical feeding assembly and feeding pipe assembly (16),
Training seedling dish (12) comprises outside framework (12), the cuboid that outside framework (12) is opened top, the inside of outside framework (12) from top to bottom successively level be fixed with first can sepage dividing plate (6), second can sepage dividing plate (7) and the 3rd can sepage dividing plate (8), thus form three layers of seedbed frame in the inside of described outside framework (12), describedly can to make by degradation material by sepage dividing plate, the inside of outside framework (12) can the below of sepage dividing plate (8) be liquid collecting chamber (17) the 3rd, the bottom righthand side of described liquid collecting chamber (17) is provided with liquid collecting chamber liquid outlet (14), the inner vertical of outside framework (12) is provided with multiple vertical dividing plate (2), vertical dividing plate (2) from the At The Height of the end face of the cuboid of described outside framework (12) through first can sepage dividing plate (6) and second can sepage dividing plate (7) extend to the 3rd can sepage dividing plate (8), thus the inner space of outside framework (12) is divided into multiple training seedling cell (1), the low order end of the inner space of described outside framework (12) is provided with discharge opeing lattice (15), the first liquid-impermeable dividing plate (9) is disposed with from top to bottom in described discharge opeing lattice (15), second liquid-impermeable dividing plate (10) and the 3rd liquid-impermeable dividing plate (11), described three liquid-impermeable dividing plates (9, 10, 11) left part is from left to right downward-sloping, right part level, described three liquid-impermeable dividing plates (9, 10, 11) left part respectively with described three can sepage dividing plate (6, 7, 8) right part termination, described three liquid-impermeable dividing plates (9, 10, 11) right part is all fixedly connected with outside framework (12), described three liquid-impermeable dividing plates (9, 10, 11) described discharge opeing lattice (15) are divided into three the discharge opeing compartments corresponding with described three layers of seedbed frame, leakage fluid dram (13) is provided with on the right side of each discharge opeing compartment,
Every layer of three layers of seedbed frame accordingly can sepage dividing plate (6, 7, 8) top is provided with nutrient chemical supply pipe group (3), the quantity of described nutrient chemical supply pipe group (3) is three, each training seedling cell (1) that described nutrient chemical supply pipe group (3) extension in every layer penetrates in the layer of place, described nutrient chemical supply pipe group (3) has two supply pipelines, described two supply pipelines are separately positioned on front side wall and the rear wall place of respective cells, and described two supply pipelines are respectively arranged with front liquid outlet (31) and rear liquid outlet (32) at each training seedling cell (1) place, described front liquid outlet (31) and rear liquid outlet (32) are all inclined downward towards the inner side fluid of respective cells,
Nutrient chemical feeding assembly comprises the first nutrient chemical feedway (22), second nutrient chemical feedway (23) and the 3rd nutrient chemical feedway (24), described first nutrient chemical feedway (22), second nutrient chemical feedway (23) is connected with feeding pipe assembly (16) respectively by three supply valves with the 3rd nutrient chemical feedway (24), feeding pipe assembly (16) is connected with three described nutrient chemical supply pipe groups (3) respectively by three receiving valves, described nutrient chemical feeding assembly is also provided with nutrient chemical supplementary device (25), described nutrient chemical supplementary device (25) is respectively by three replenishment valve and the first nutrient chemical feedway (22), second nutrient chemical feedway (23) is connected with the 3rd nutrient chemical feedway (24),
The step of described method is:
Every layer at three layers of seedbed frame is respectively charged into corresponding culture matrix;
Blueberry seedling is inserted in each training seedling cell (1), make the end of blueberry seedling can sepage dividing plate (6) place first;
In the nursery first stage, control corresponding valve, make the first nutrient chemical in the first nutrient chemical feedway (22) be supplied to first can sepage dividing plate (6) top training seedling cell (1) in;
In nursery second stage, control corresponding valve, make the second nutrient chemical in the second nutrient chemical feedway (23) be supplied to second can sepage dividing plate (7) top training seedling cell (1) in;
In the phase III of nursery, control corresponding valve, make the 3rd nutrient chemical in the 3rd nutrient chemical feedway (24) be supplied to the 3rd can sepage dividing plate (8) top training seedling cell (1) in;
When nutrient chemical in every layer of three layers of seedbed frame spills in described discharge opeing lattice (15), nutrient chemical is discharged by corresponding leakage fluid dram (13); When nutrient chemical penetrates in liquid collecting chamber (17), nutrient chemical is discharged by liquid collecting chamber liquid outlet (14).
2. utilize the quick outside sprout-cultivating-bottle method of a kind of blueberry tissue culture seedling described in claim 1 and obtained culture matrix.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410001446.6A CN103704109B (en) | 2014-01-03 | 2014-01-03 | Fast rooting method for blueberry tissue culture seedlings outside bottles and rooting culture medium |
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| CN201410001446.6A CN103704109B (en) | 2014-01-03 | 2014-01-03 | Fast rooting method for blueberry tissue culture seedlings outside bottles and rooting culture medium |
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| CN103704109B true CN103704109B (en) | 2015-05-13 |
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| CN115968784A (en) * | 2023-02-06 | 2023-04-18 | 广州智源农业科技发展有限公司 | Low-cost rapid breeding method for blueberry seedlings |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1041258A (en) * | 1988-09-24 | 1990-04-18 | 株式会社小松制作所 | Put bed and seedling nursing device |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JPH03216122A (en) * | 1990-01-17 | 1991-09-24 | Iwatani Internatl Corp | Sterilization of culture liquid for soilless culture |
| JP5147796B2 (en) * | 2008-09-11 | 2013-02-20 | 日本グリーンファーム株式会社 | Plant cultivation system and plant cultivation plant |
| JP5707065B2 (en) * | 2010-07-06 | 2015-04-22 | 国立大学法人高知大学 | Plant cultivation method |
| CN102860250B (en) * | 2011-07-08 | 2014-10-22 | 乔奥华塑胶制品(深圳)有限公司 | Culture solution planting device |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1041258A (en) * | 1988-09-24 | 1990-04-18 | 株式会社小松制作所 | Put bed and seedling nursing device |
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