CN1041258A - Put bed and seedling nursing device - Google Patents

Abstract

Seedling nursing device of the present invention has incubator, an end sealable and that all good culture medium carrier of moisture capacity and hydrophily is housed and is connected with this incubator and can freely installs and removes fungi-proofing connector, gas supply system and the several culture medium solution feed system that carries out sterilization treatment.Also have the culture apparatus that the tissue culture body is accommodated in the lump together with culture fluid, the sorting unit that comprises concentration adjusting device and sorting tissue culture body.

Description

Put bed and seedling nursing device

The present invention relates to put bed and seedling nursing device,, make it put bed and cultivate the device on the base and in incubator, carry out the cultivation of tissue culture body, the environment control unit of domestication continuously in nutrient solution particularly about the sorting cells body.

In recent years, about cultivating the technological research paid more and more attention of plant biological of useful plant.It is to take out by the various cells that will form plant corpus, histoorgan, as required, after the various artificial treatment such as enforcement cytomixis, the processing of variation source, hereditary factor control, makes the process of plant corpus regeneration, improves the subject of plant.

For example, the seedling-cultivating method that adopts the stem class to cultivate as shown in figure 26, is made up of following several stages, cuts certain established part of plant that is:, makes the plant tissue that therefrom takes out put on the agar medium bed in container and makes breeding stage of its breeding; Subsequently it is transplanted to the development stage that makes its germination in the other container; Again it separately is transplanted to but other container in make taking root the stage that it takes root; And, rinse agar well back and in the domestication device, make it be in dark moist environment short its further to take root, make it progressively adapt to the domestication stage of natural environment thereafter again by extracting in the container.After the domestication, it is transplanted to makes it cultivate into seedling in the seedling-growing container again.Because in each stage of breeding, germinateing, taking root, must use the agar medium that contains optimal components to cultivate respectively, therefore, its shortcoming is each stage of cultivating, must at least once transplant operation, so need more manpower and higher cost.

In addition, in each stage, for making gas and medium component keep optimum state at any time, need to change medium component the replenishing of promoting the circulation of qi body of going forward side by side, yet, because in vitro additional the and discharging of gas is all very difficult, just must transplant if will change medium component during again owing to the use agar medium, and consider and be convenient to operation and reduce plant corpus and damage these problems, in each stage, just only select a kind of medium component as far as possible, therefore, the root of particularly giving birth in the stage of taking root often can not be grown fully because of oxygen is not enough and be caused the deformity of growing up.

In addition, move into domestication during the stage, it is transplanted in the implantation medium of vermiculite etc. the seedling that will take root, yet, owing in used agar medium of the stage of taking root, contain sugar, for prevent mouldy just must agar is clean.This clean operation should spend many manpowers, and root is also impaired easily, also often becomes poky reason.

Therefore, the inventor once provided following apparatus, that is: the culture medium carrier that will soak into culture medium solution is seated in the airtight container, then tissue culture bodies such as indefinite bud, adventitious embryo are transplanted on this culture medium carrier, by replenish or discharge culture medium solution to these culture medium carriers and replenish or amount discharge container in gas componant, make and cultivate necessary nutrient solution and gas keeps optimum state at any time and it is taken root; Simultaneously, the inventor also provides and has made its domestication device that progressively adapts to natural conditions (seeing Japanese patent application 86-187691 and 86-187692).

Use this device can bring many excellent results, as: need not to transplant operation, and can make gas and medium component keep optimum state, and the tissue culture body is grown well and be convenient to operation.In addition,, make its domestication under the kilter of the growth that can in not, be completely cured owing to when domestication, also need not to transplant, thus low-cost growing seedlings at a high speed is achieved, or the like.

Yet, because the extremely difficult intrusion that prevents germ of tissue culture so operation must be carried out, also needs skilled operating personnel in clean bench.Owing to should carry out repeatedly adjustment, the supply of gas, the discharge of culture medium solution, prevent the intrusion of germ simultaneously again, this just brings very big difficulty to operation, and Here it is hinders one of actual reason of using of this device.

In addition, when coming the breeding plant body,, mainly use stem apex and axillalry bud from the viewpoint that prevents to make a variation with tissue culture method.

Past, a kind of tissue culture method that uses stem apex, axillalry bud etc. to carry out is to cut these positions earlier, the plant tissue that takes out in the thing is placed on 2 being cut by these again, make its organization healing in the agar medium of 4D etc., make these callus breedings with liquid nutrient medium after, after sieve.First filter that this sieve is made by the screen cloth of 100 μ m and form by second filter that the screen cloth of 50 μ m is made.

Subsequently, be placed on and do not contain 2, wash in the liquid nutrient medium of 4D, after carefully cleaning, be placed on again in the new liquid nutrient medium and make it develop into adventitious embryo remaining in consistent (50 μ m＜X＜100 μ m) cellule piece of size on second filter.

And then, obtain the consistent adventitious embryo of size with the filter filtration.

At last, Yi Bian observe the shape and the color of adventitious embryo,, make it put bed on medium again Yi Bian carry out sorting.

In such operation, the problem of existence is that the whole operation process is almost all carried out by hand, and for fear of by living contaminants, must carry out in clean bench, and needs careful and skilled operating technology.

Especially when putting bed, owing to will plant singly on medium a large amount of tissue culture body and functions of cultivating are manual in incubator, just making this put an operation becomes and hinders a large amount of keys of handling in the whole operation process.

In view of the foregoing, the objective of the invention is to: a kind of seedling nursing device is provided, and it can prevent the intrusion of germ and realize growing seedlings at a high speed with low cost.

In view of the foregoing, another object of the present invention is that the separation operation of raising tissue culture body and the operating efficiency of putting an operation are realized sterile working simultaneously, improves the efficient of tissue culture.

Therefore, the present invention has the incubator that can seal, the all good culture medium carrier of moisture capacity and hydrophily is housed in the case, also have and make an end be connected to this incubator and can freely installing and removing, carry out the fungi-proofing connector of sterilization treatment, and have gas supply system and a several culture medium solution feed system, so that according to cultivating the different phase that bulk-growth is grown, with the other end of fungi-proofing connector with being connected one of in above-mentioned gas supply system and the several culture medium solution feed systems, thereby realize supplying with to incubator, discharge gas and culture medium solution.

If adopt the device of this structure,, thereby be easy to incubator is carried out sterilization treatment owing to incubator can freely be installed and removed with respect to fungi-proofing connector; Again because sterilization treatment also can be carried out in the coupling part, so can be after being transplanted to tissue culture bodies such as indefinite bud, adventitious embryo on the culture medium carrier, under germ-free condition, supplying with, discharge culture medium solution with proper composition and gas successively and be convenient to operation according to the different phase of growing.

In addition, owing to need not to transplant operation during the stage to domestication,, remove the sugared culture medium solution that contains that is used to take root so can culture medium carrier be cleaned in that the seedling that has taken root is kept intact under the motionless state from the step transition of taking root.After this, just can supply with successively and be suitable for the culture medium solution that seedling is used, like this, just can make root without any damage.

So,, and under the state of not encroached on, constantly grow by germ for the moisture avoiding the seedling lack of water to be fading supplying with and the culture medium solution of supply just can be absorbed well by root.

In addition, because the lack of water that produces because of the transfer in the stage phenomenon that phenomenon or termination grow of being fading is not constantly grown well, that is to say, can under lower humidity and stronger illumination condition, tame, can realize cheaply high-speed rapid growth and be convenient to operation.

In addition, the bed apparatus of putting of the present invention has following a few part: the culture apparatus that contains tissue culture body and culture fluid; The sorting unit that comprises concentration adjusting device and sorting tissue culture body, this sorting unit will wherein contain the tissue culture body sent here from culture apparatus, concentration certain culture liquid sends by a certain amount of portion aly and carries out sorting; The tissue culture body time interval in accordance with regulations that makes sorting falls into medium by the front end of carrier pipe makes it put the bed unit of putting of bed.

This puts bed apparatus owing to adopt sorting unit to carry out sorting, can prevent that the tissue culture body that makes a variation from putting bed, and can carry out sorting at an easy rate under aseptic condition and put bed.

In addition, can occur at interval by the tissue culture body that concentration adjusting device is regulated in the carrier pipe.If put bed at interval, then can realize putting one by one bed at an easy rate by this.

Brief description of drawings is as follows:

Fig. 1 is the principle schematic of the seedling nursing device of the expression embodiment of the invention, Fig. 2 (a)～(c) illustrates the incubator of this seedling nursing device, Fig. 3 illustrates the hermetically-sealed construction of this incubator, Fig. 4 (a) and Fig. 4 (b) illustrate the fungi-proofing connector of this seedling nursing device, Fig. 5 illustrates air supply system, Fig. 6 illustrates the bactericidal unit of air, Fig. 7 illustrates the nutrient solution feed system, Fig. 8 illustrates the aqua sterilisa feed system, Fig. 9 illustrates solution feeding system and mixing arrangement, Figure 10 is another version of the hermetically-sealed construction of incubator, Figure 11 is the layout drawing of incubator when utilizing sunlight collecting, Figure 12 (a)～(c) and Figure 13 (a)～(c) are respectively the version of the cooling structure of incubator, Figure 14, Figure 15 (a) and (b) be in-built other versions of incubator, Figure 16 is another version of nutrient solution feed system, Figure 17 is total figure of the seedling nursing device of the embodiment of the invention, Figure 18 is the schematic diagram of putting bed apparatus of the embodiment of the invention, Figure 19 puts the enlarged drawing of the sorting unit of bed apparatus for this, Figure 20 is the enlarged drawing of distinguishing color part of this sorting unit, Figure 21 (a) and (b) for putting the vertical view and the front view of bed unit, Figure 22 (a) and (b) entrance door of putting a chamber of putting bed unit is shown, Figure 23 illustrates the connector that is contained in the incubator, Figure 24 is another version example of incubator, Figure 25 illustrates another embodiment of the present invention, and Figure 26 adopts the schematic diagram of the seedling-cultivating method of Shoot Tip Culture for expression.

Below, in conjunction with the accompanying drawings embodiments of the invention are described in detail.

Fig. 1 is the principle schematic (Figure 17 is its total figure) of the seedling nursing device of the embodiment of the invention.

This seedling nursing device is that adventitious embryo that tissue culture method is cultivated etc. is put bed, does not transplant from the stage of taking root to the domestication stage and carries out continuously, and the supply of control nutrient composition and gas, discharge the device of usefulness.As shown in Figure 1, this device by all the other 5 omissions of six culture apparatus 100() and gas supply part 200, nutrient solution supply unit 300, control part 400 and the 1st and the 2nd wash mill 202,302 form.This culture apparatus 100 is made up of incubator 106, illuminace component 107, temperature control unit 108.Wherein, incubator 106 is included in air supply pipe 102, blast pipe 103, nutrient solution supply pipe 104 and the nutrient solution discharge pipe 105 that the top has the fungi-proofing connector 101 that can freely install and remove; Illuminace component 107 is supplied with the needed illumination of incubator; The temperature of temperature control unit 108 control incubators.Gas supply part 200 and nutrient solution supply unit 300 have first common concerning each culture apparatus the total pipeline 201 and second total pipeline 301 respectively, and can be connected with each fungi-proofing connector 101 respectively with freely installing and removing, the the 1st and the 2nd decontaminating apparatus is respectively applied for cleans above-mentioned first total pipeline and second total pipeline, make the tissue culture body at incubator 106 built-in beds, all need not to transplant from the stage of taking root to the domestication stage, different phase according to the growth of tissue culture bulk-growth, pass through common total pipeline 201 respectively by gas supply part and nutrient solution supply unit, 301 supply with the gas of the required composition of each nutrition device and the nutrient solution of required composition, simultaneously, when will change nutrient composition or gas componant, just total pipeline is rinsed well with the first and second total pipeline decontaminating apparatus, keep germ-free condition, thereby realize high-speed growing and taming.

Fig. 2 (a) to (c) is respectively end view, vertical view and the A-A cutaway view of incubator.As shown in these figures, each incubator 106 is made up of the lid 106b that box main body 106a is connected with gas-tight seal with it.In box main body, be paved with polywater body 110, for preventing that root is entangled on this polywater body, be provided with anti-root layer 111, in the volume part that separates with dividing plate 112, load the culture medium solution carrier made from polyester silk floss or vermiculite and so on material 113 simultaneously, draw nutrient solution discharge pipe 105 in the bottom by flange 114, by being contained in the flange so that the capillary module 115 that contacts with polywater body 110 is discharged liquid.In addition, draw air supply pipe 102, nutrient solution supply pipe 104, blast pipe 103 respectively in the side.

This air supply pipe 102 faces the top of the air intake duct 102a in box main body, to supply with and remain in nutrient solution in the hydrops district 117 that surrounds with housing 116 or water by nutrient solution supply pipe 104 drinks up and atomizes the tissue culture body on the supply culture medium solution carrier 113.In addition, this housing 116 is made up of fixed head 116a and adjustment plate 116b, and wherein adjusting plate 116b can be movable with internal magnet 118a.By the external magnet 118b of mobile incubator lateral wall, can regulate the height of adjusting plate 116b, and can realize the adjusting of atomization quantity by the height of water level in the adjustment housings and the spray volume of gas, thereby being become, the control of liquid measure is easy to.In addition, adjust plate 116b if improve, mist is bumped against with it, then the fog particles that size is big is returned hydrops district 117 again and is atomized, has only the little mist particulate of size to be full of in the incubator 106.

In addition, the direction of air supply pipe 102 head nozzles both can change as required, also can make variable.

Have, the inner of blast pipe 103 has funnelform suction inlet (not shown) and gets rid of vaporific sediment with sedimentation again.

In addition, the joint of box main body 106a and lid 106b, as shown in Figure 3, the main flange 120a and the lid flange 120b that make at box main body and lid periphery are separately linked together by O-ring seals 121, will these flanges around with snap ring 122 chuckings to form airtight sealed structure.Have, the lid flange makes again The shape section makes main flange can insert the lid flange recess, this lid flange

The outer side edges of shape section can prevent to enter O-ring seals 121 from the water droplet of outside, and its inner side edge then can prevent to enter O-ring seals 121 from the water droplet of inside.

Shown in Fig. 2 (c), the flange 114 interior capillary modules 115 that are arranged on incubator 106 bottoms are all made with materials such as porous ceramics or cotton, paper with the polywater body.And nutrient solution by anti-root layer 111 and polywater body 110 pinching culture medium solution carriers 113.Like this, capillarity is strong more, and the saturation pressure force is just high more, and air is difficult to by having only moisture to pass through; So by the pressure in the incubator 106 is raise a little, nutrient solution discharge pipe 105 is attracted, nutrient solution is successfully discharged.

Have again, at the sidewall mounting temperature sensor 123 and the humidity sensor 124 of incubator.The output of these sensors is sent to environment control part 125, and is treated there as control data uses such as control intensity of illumination, air demand sizes.This environment control part 125 is by control part 400 centralized Control.

, when incubator was penetrated by illumination, because incubator top is light transmissive material, temperature just rose thereupon.For this reason, implement temperature control with temperature control part 108.The temperature control part 108 that the instruction of sending by above-mentioned environment control part 125 is moved, as shown in Figure 1, by cooling water preparation facilities 128a, be installed in the cooling water nozzle 127 above the incubator and the bosh 128b that is installed in below the incubator forms.Overflow to the bosh 128b from incubator top by making from the cooling water of cooling water nozzle 127 ejection, thus the cooling incubator around, realize control to its internal temperature.

Secondly, Lighting Division is made up of with filter 130 light source 129 and adjusting that metal halide lamp makes.Regulate with filter 130 and control, control the luminous intensity and the Wavelength distribution of irradiation incubator 106 according to the needs of the different phase of growing by above-mentioned environment control part 125.

Have, fungi-proofing connector 101 as Fig. 4 (a) with (b), is made up of connector main body 101a that has steam supply port 131 and steam outlet 132 respectively and lid 101b again.The end of this connector main body 101a and lid 101b has flange 133a and 133b respectively, simultaneously, the air supply pipe 102, blast pipe 103, nutrient solution supply pipe 104, nutrient solution discharge pipe 105 of valve 134a, 134b are housed in this connector main body 101a and lid 101b.During connection, shown in Fig. 2 (a)～(c), at first with incubator this part (connector main body this part), after loading onto the culture medium solution carrier, intactly in autoclave, sterilize, with after putting bed apparatus (not shown) etc. and in clean bench, adventitious embryo being put bed, valve 134a at each pipeline, under the 134b closing state, connector main body is lumped together with the connector lid, connect by bolt 135, under the situation that has the gap between two flanges (as Fig. 4 (a)), by steam supply port 131 air feed, from 132 exhausts of steam outlet, feed 121 ℃, 2Kg/Cm ²High-pressure steam 10 minutes.Like this, all can be sterilized in the pipeline valve 134a, 134b from flange 133a, 133b.

, unclamp bolt 135, connector main body and lid are slided on slideway 136, realize being tightly connected shown in Fig. 4 (b), open valve 134a, 134b again, just realized the connection under sterilising conditions when both make flange 133a, 133b at a touch thereafter.

Below, illustrated with regard to gas supply part 200.

Gas supply part 200, as shown in Figure 5, prefilter 202, first filter 203, pump 204 and storage are housed successively by the gas tank 205 of the air of pump 204 supplies, adjusting device 206, drier 207, second filter 208 and the 3rd filter 209 etc. that adjusting pressure is used.Connect first total pipeline 201 of supplying with each culture apparatus 100 gas here again.Wherein, drier 207, second filter 208, the 3rd filter 209 also are equipped with the joint 210,211,212,213 of the band check valve that can change respectively.In addition, the three-way connection 216,217,218,219,220 of band check valve is housed in ducted many places, during for retubing pipeline is carried out sterilization treatment and use.That is to say, for example, when changing second filter 208,, discharge, the joint 212,213 of band check valve is sterilized through the three-way connection 218 of band check valve by the dry pressure-air of three-way connection 217 feedings of band check valve.At this moment, can around the second and the 3rd filter 208,209, load onto the heater (not shown) so that it is carried out high-temperature sterilization.In addition, also can adopt the method for these filters that are arranged side by side, when normally using air feed, another be changed sterilization.

In addition, the sterilization of the prefilter 202 and first filter 203 also can be loaded onto heater as required and carry out around it.

Have, as shown in Figure 6,206 these sections can be provided with air sterilization portion 221 from air accumulator 205 to adjusting device, so that the sterilization of pressurizeing (121 ℃, 10 minutes) again.This air sterilization portion 221 is from air accumulator 205, comprise the pump 223 air sent through air intake duct 222, the reel cooling tube 228 and the sterilization tank 229 of heating tube 225 that heating element heater 224 makes, pressure regulator 226, band fan 227 all around, at last, sterilization tank 229 connects with adjusting device 206.

By the gas (being air here) of this gas supply part, respectively through the fungi-proofing connector 101 of choke valve 230 and filter 231 each culture apparatus of feeding, supply in the incubator 106 by air supply pipe 102 again from first total pipeline.The adjusting of air demand is carried out with adjusting device 206.Also can replace choke valve 230 with damping hole.

In addition, first decontaminating apparatus 202 that cleaning is used comprises: joint 233 and vent valve 233b that the band check valve is housed by valve 232 at the end of first total pipeline, carry dry pressure-air from the three-way connection 234 that is loaded on this total pipeline other end (that end of gas supply part) to the joint 233 of above-mentioned band check valve, in order to sterilize.

The gas of being discharged through blast pipe 103 by incubator is discharged to gas discharge section 237 by gas outlet pipe road 235.This gas outlet pipe road 235 is connected to first through valve 236 and cleans on the water supplying pipe road 240, washes and make water be discharged to gas discharge section 237 with clean water pipeline 235 is purified.238 is blow vent, and 239 is rhone.

Below, nutrient solution supply unit 300 is illustrated.Nutrient solution supply unit 300, as shown in Figure 7, consist of the following components: the solution feeding system 304 of aqua sterilisa feed system 303, base feed solution, pH value regulator solution feed system 305, material solution and pH value regulator solution and aqua sterilisa mixed and be adjusted to the mixing arrangement 306 of the nutrient solution that is suitable for cultivating usefulness and the various nutrient solutions that will mix are supplied with the nutrient solution feed system 307 of each culture apparatus 100.

Aqua sterilisa feed system 303, as shown in Figure 8, have a sterilization portion 319, this sterilization portion composed as follows: three-way connection 316a and (when changing filter) filter 316 that can bypass, the joint 317 and the three-way connection 318 of band check valve all are equipped with in joint 315, the two ends that are connected to joint 308, valve 309, the pressure regulator 310 of the band check valve on the water pipe (not shown), the filter 311 that uses active carbon, first and second exchange resin towers 312,313, three-way connection 314, band check valve.The aqua sterilisa that has carried out sterilization treatment through sterilization portion 319 is stored in the aqua sterilisa supplying tank 321 by valve 320, on the other hand, also can directly clean water supplying pipe 336 by first clean water supplying pipe 240, the second clean water supplying pipe 390 and the 3rd and directly supply with.322 is float, and the water yield of aqua sterilisa supplying tank is opened valve 320 automatically once reducing.In addition, the 323rd, in order from aqua sterilisa supplying tank 321, to extract first pumping plant that pure water is used out.This first pumping plant is cleaned water supplying pipe 390 and is connected with solution feeding system 304, mixing arrangement 306, the 3rd total pipeline 324, second.Have again, also the pure water jar can be connected on the distilled water pipeline replacing aqua sterilisa service 319 by fungi-proofing connector, yet, because of usage amount is big, not too reasonable.

Have again, solution feeding system 304, as shown in Figure 9, it is composed as follows: second pumping plant 371 of the joint 370 of the band check valve that can be directly connected on the original fluid container (not shown) is housed, and itself and filter 372 have connected to form the first sterilization pipeline 373 successively; The stoste of being supplied with by original fluid container is supplied with the 1st～6 NaOH solution tank NaOH 325 by the 3rd total pipeline 324 after 373 sterilizations of first sterile tube.Joint 376,377, three-way connection 378,379, the rhone 380 of band check valve have been installed in this first sterile tube 373 too; Filter 372 also makes the form that can carry out sterilization treatment when changing.

In addition, the 3rd total pipeline 324 becomes the first and second branch pipe(tube) 324a, 324b by valve 331a, 331b branch respectively; 324 the other end passes to aqua sterilisa supplying tank 321 through valve 341.

Have again, by aqua sterilisa supply pipe 319(with reference to Fig. 8) branch form the 3rd clean water supplying pipe 336 and be branched off into the 3rd branch pipe(tube) 336b through valve 337, also be branched off into aqua sterilisa conveying end 339 simultaneously through valve 338, pass to pH value regulator solution feed system 305 through valve 340.Each solution supplying tank 325 is connected on the first branch pipe(tube) 324a by three-way connection 332 separately, and the three-way connection 342 by separately is connected on the second branch pipe(tube) 324b simultaneously.

And each solution supplying tank 325 is connected on the 3rd branch pipe(tube) 336b by shower valve 343 and dump valve 344, can supply with spray nozzle 343a by shower valve 343 and clean the pure water of using in the jar.The 3rd branch pipe(tube) 336b also is connected to rhone 335 by valve 345.The stoste of supplying with through the 3rd total pipeline 324 herein, by opening one of valve 331a and six NaOH solution tank NaOHs 325, for example the three-way connection 332 of the 3rd NaOH solution tank NaOH 325 is supplied with corresponding solution supplying tank 325 through the first branch pipe(tube) 324a.Herein, 333 is gas vent, and 334 is valve.

Much less, the solution that has nothing in common with each other can inject each solution supplying tank successively, inject solution once finishing to open valve 344 to a solution supplying tank, aqua sterilisa is flow in the 3rd total pipeline 324 by aqua sterilisa supply pipe 319, be discharged in the rhone 335 by above-mentioned supply valve 331a, thereby can clean the first branch pipe(tube) 324a.In addition, supply with solution to mixing arrangement 306, can carry out through the 3rd pumping plant 346 by the second branch pipe(tube) 324b by opening the three-way connection 342 that is contained in each solution supplying tank 325 top from each of these solution supplying tanks 325.

Herein, the 3rd pumping plant 346 is equipped with three-way connection 348a, 348b at its two ends, and rhone 349 is housed simultaneously, can carry out sterilization treatment by these three-way connections.

Like this, in case finish the supply of solution by the 3rd pumping plant 346 to mixing arrangement, the solution supplying tank 325 that becomes slack tank just will be cleaned water by shower valve 343 by the 3rd branch pipe(tube) 336b and supply with spray nozzle 343a it is rinsed well.At this moment, valve 345 cuts out in advance, and after cleaning, opens valve 344 and 345, and detergent remover is discharged in the rhone 335.

In addition, because per three of solution supplying tank is formed two groups of one group of ground formation, so be provided with and the 3rd pumping plant 346 identical the 4th pumping plants 347.The reason that is divided into two groups is: make the capacity of the jar of each group have difference, thereby can the solution that usage amount is big pack big capacity jar into, will amount little and solution that matter the is good low capacity jar of packing into.

Have, pH value regulator solution feed system 305 is made up of the alkali supplying tank 350 and the sour supplying tank 351 that have with solution supplying tank 325 identical construction again.About also carrying out equally with cleaning of tube connector in the jar with solution supplying tank 325.

In addition, alkali supplying tank 350, sour supplying tank 351 are connected on the mixing arrangement by the 5th and the 6th pump mechanism 352,353 respectively.The structure of the 5th and the 6th pump mechanism is also identical with third and fourth pumping plant 346,347.

Mixing arrangement 306 is by forming with the lower part: blending tank 354, the dilution ware 355 that is arranged on blending tank top, damper 356, with magnet 357 from the rotating spoon 358 of external drive, pH value sensor 359, through valve 360 be connected to aqua sterilisa supply pipe 319 for jar in the spray nozzle 361 and the rhone 362 of clean usefulness.This mixing arrangement 306 is supplied with nutrient solution feed system 307 by being connected with the 7th pumping plant 364 by the pipette 363 of drawing near the blending tank bottom side with the nutrient solution that is mixed into required composition.

Have, the effect of dilution ware 355 is again: if stoste is directly dropped into often generation precipitation in the jar, dilute while therefore drop into pure water on this dilution ware.

The 365th, filter, the 7th pumping plant 364 and its common second sterilization pipeline 366 of forming, its structure used first sterile tube 373 with by above-mentioned original fluid container (not shown) supply stoste the time is identical.

Like this, as shown in Figure 7, nutrient solution is just charged into nutrient solution through the first nutrient solution pipe 382a the 1st to the 3rd nutrient tank 381 of nutrient solution feed system 307 by mixing arrangement 306.

Nutrient solution feed system 307 is realized supplying with nutrient solution to incubator 106 by aforementioned second total pipeline 301, this second total pipeline 301 comprises: construct identical the 1st to the 3rd nutrient solution supplying tank 381 with aforementioned solution supplying tank, the 2nd nutrient solution supply pipe 382b and the 3rd sterile tube 383 that will be sent by the nutrient solution that the 1st to the 3rd nutrient solution supplying tank is drawn.Have, the 3rd sterile tube 383 is the same with first and second sterile tubes to have pump mechanism 384 and filter 385, is connected on second total pipeline 301 by valve 386 again.

In addition, the end of second total pipeline 301 is equipped with the joint 388 and the rhone 389 of valve 387, band check valve, and the other end is connected on the 3rd sterile tube 383, is connected on the first clean water supplying pipe 240 of being drawn by aqua sterilisa supply unit 303 through valve 391 again on the other hand.This second total pipeline 301 has constituted the second total pipeline decontaminating apparatus 302 that can clean with aqua sterilisa.

In addition, nutrient solution is discharged by nutrient solution discharge pipe 395 by incubator 106, and its end communicates with gas vent 397 through valve 396.398 is rhone.In addition, the other end of this nutrient solution discharge pipe 395 is connected on the first clean water supplying pipe 240 through valve 399.

Have, the 3rd total pipeline 324 is connected on the second nutrient solution supply pipe 382b through valve 393 again, also can directly be added the solution (with reference to Figure 17) of regulation by the solution supplying tank.

If install with this, because incubator can freely be installed and removed through fungi-proofing connector, make incubator be easy to carry out sterilization treatment, and it is connected with the solution supply unit with gas supply part under germ-free condition, thereby can prevent pathogen contamination significantly.

In addition, because several nutrient tanks can be connected on the incubator by total pipeline with freely switching, and total pipeline can rinse well with the clean water of aqua sterilisa composition after each change nutrient solution, so can make needed pump mechanism number minimizing.Have, for such device, " fungi-proofing " is considered to most important again, because fungi-proofing equipment increases with the use number of pump with being directly proportional, the number that therefore reduces pump installation is effective especially.In the present embodiment,, two cover pumps must be arranged owing to used two groups of different jars of capacity, if it is use the jar of same capability entirely, passable once the cover pump.

Also identical therewith about supplying with the employed pump installation of solution to mixing arrangement by the solution supplying tank.

Have again, in this device, since in mixing arrangement, be easy to the solution by the various compositions that come in several solution supplying tanks is mixed, can carry out meticulous adjusting according to the needs of different vegetative stages, thus promote growing and taming of tissue culture body.

In addition, the parts such as filter that must change in use are that the joint by the band check valve connects fully, so fungi-proofing during to replacing also is effective.

Have again, in the present embodiment,, also can use the stable polyester silk floss that is difficult to be dissociated into ion, peat bog etc. though use vermiculite as culture medium carrier.During sterilization operation or tissue culture, about the ion situation of disassociation in solution, can pass through to measure in advance in addition, the ion in the adjusting nutrient solution is formed keeps under suitable condition it.

The airtight sealed structure of incubator is not limited to the sort of structure shown in Figure 3, and its structure also can make as shown in figure 10, two O-ring seals 121 of packing between main flange 120a ' and the lid flange 120b ' ' and, S is at interval arranged therebetween.

Illuminating light source 129 is not limited to metal halide lamp, also can utilize sunshine.At this moment, as shown in figure 11, for the efficient that improves light with effectively utilize shared space, also can tilt several incubators 106 are installed at two root posts 140.141 is the cooling water nozzle, and 142 is bosh, and 143 is cooling water pipe, makes cooling water be easy to flow.

Shown in Figure 12 (a) and (b), (c), also can on incubator 106, make frame 144, in this frame, flow into cooling water by cooling water supply port 145, discharge by cooling water outlet 146 again.

Shown in Figure 13 (a) and (b), (c), also can make incubator not only on incubator, also add upper ledge in its side, cooling water is discharged through cooling water outlet 149 by cooling water supply port 148.

As shown in figure 14, also heat exchanger 150 can be set in incubator 106, carry out adjustment with fan 151 disturbance air.In addition, this fan also can use for form moving air in incubator, seedling can be placed under the stimulation of wind in the domestication stage.If can cultivate like this, the healthy and strong seedling of ability irriate.

Have, this fan can be used for replacing magnetic stirrer and make it reach fungi-proofing fully again.

Also can will make triangularity above the incubator,, can also get rid of water droplet with the electromagnetism wiper so that the water droplet of case inner face flows down.

Incubator shown in Figure 15 (a) and (b) replaces the gas outlet pipe and the nutrient solution discharge pipe of incubator with a discharge pipe 153 that reversal valve 152 is housed, drive reversal valve 152 with solenoid 154, reversal valve one rises, exhaust nozzle 155 just becomes closed condition, and apocenosis mouth 156 is opened, and nutrient solution is just discharged by discharge pipe 153.On the contrary, reversal valve one descends, and gas just is discharged from.In addition, can on the bottom surface of incubator, process groove 157, capillary module 158 compressions are seated in wherein, replace the capillary module in the original structure flange.

In the present embodiment, discharge opeing is discharged by sump pit.As shown in figure 16, also it can be stored in the Drainage tank 500, turn back in the culture apparatus again.Like this, can adjust liquid level, also can in pipeline, install adsorbent equipment additional, be used for adsorbing waste material to reclaim, to utilize again according to the different phase of growing.Wherein 501 is pump mechanism.

Below, with reference to accompanying drawing second embodiment of the present invention described in detail.

Figure 18 is the overall construction drawing of putting bed apparatus of the expression embodiment of the invention.

This puts bed apparatus is the device that is used for the adventitious embryo of genseng is put bed, is to putting the device that bed all carries out automatically from callus.It consists of the following components: with nutrient solution cultivate callus and generate adventitious embryo culture apparatus 1100, will contain adventitious embryo nutrient solution by culture apparatus 1100 measure in accordance with regulations that a part of ground takes out, a sorting unit 1200 of sorting and will put one by one with the adventitious embryo that sorting unit 1200 sub-elects and put bed unit 1300 bed on each unit are of incubator.

Culture apparatus 1100 is made up of culture tank 1107, it is connected on the nutrient solution feed system (not shown) by valve 1101, be connected respectively on first discharge pipe 1104 and the rhone 1105 by valve 1102 and valve 1103 again simultaneously and be equipped with and supply to drop into the sterility cover 1106 that callus is used, stir a nutrient solution that contains callus 1109 that drops in the jar with propeller 1108, short its quickens to cultivate under aseptic condition, with liquid suction pipe 1110 the adventitious embryo nutrient solution that forms through cultivation delivered to sorting unit 1200 simultaneously.

Liquid suction pipe 1110, as shown in figure 19, it is composed as follows: refill 5 rollers free to rotate 1111 through bearing pin 1112 on the pivotal parts 1113 that itself can rotate freely, every the stipulated time in roller 1111 and 1114 pushings of guide rail, because volume-variation is so can extract a certain amount of adventitious embryo nutrient solution in the culture tank at every turn.

Sorting unit 1200 as shown in figure 19, by filter 1210, concentration adjusting device 1220 with distinguish that look device 1230 forms.It is to be used for sub-electing from the adventitious embryo nutrient solution of being extracted out by liquid suction pipe 1110 through valve 1201 the good adventitious embryo that can put bed.

Filter 1210 comprises second filter 1212 that first filter 1211 be made up of 100 μ m screen clothes and 50 μ m screen clothes are formed.Remove the adventitious embryo that bed is put in excessive being unsuitable for first filter 1211, discharged by second discharge pipe 1214 through valve 1213, the suitable adventitious embryo (50 μ m＜a＜100 μ m) of size that will stay simultaneously on second filter is sent by carrier pipe 1216 through valve 1215.The 3rd discharge pipe 1218 that connects through valve 1217 is to be used for the pipeline that utilizable adventitious embryo foldback with surplus goes back, promptly, when the concentration of concentration adjusting device 1220 surpasses the concentration of defined, just these utilizable adventitious embryos are not delivered to concentration adjusting device 1220 and send back to as residue.To contain in addition and deliver to the first circulating pipe 1219b by the culture fluid of the less adventitious embryo of the size of second filter through valve 1219a it is turned back in the culture tank 1107.Have again, the 1240th, when the adventitious embryo excessive concentration in the culture fluid, be used for supplying with the pure water of adjusting usefulness or first adjustable pipe of nutrient solution, the 1241st, valve.

The structure of concentration adjusting device is as follows: the window 1222 that has pair of light-transmissive on the relative two side of light tight container 1221, at window on one side first light-emitting component 1224 is set, condenser 1223 also is housed therebetween, at the another side window first photo detector 1225 is set, measures the concentration of adventitious embryo according to the luminous flux of first photo detector, 1225 receptions.The 1226th, the screw of stirring usefulness drives with motor 1227.The structure of this part makes and stirs with screw with this stirring on one side, Yi Bian the concentration of mensuration before the concentration that reaches regulation, is all supplied with culture fluid or pure water by second adjustable pipe 1228.When a concentration that reaches regulation, shut off valve 1229 stops to supply with at once, and the adventitious embryo concentration in the container is remained unchanged always.

Like this, the culture fluid that contains adventitious embryo that concentration has been mixed up by the constant displacement pump 1243 that drives with motor 1242 is delivered to and is distinguished look device 1230, makes it only contain an adventitious embryo at every turn.Wherein 1244,1245 all is check valve, the 1246th, and valve.

Shown in the enlarged drawing of Figure 20, with this constant displacement pump 1243 culture fluid of definite capacity is delivered in the detector tube 1231 of light-permeable by a part, the light that is sent by second light-emitting component 1232 shines on the culture fluid of sending here by condenser 1233, detect the light that is seen through with second photo detector 1236 by filter 1234 and condenser 1235, thereby measure color and size by the adventitious embryo in the detector tube 1231.

In addition, the 1237th, triple valve is when second photo detector 1236 detects the adventitious embryo H that is passed through, these triple valve 1237 actions are connected and are put bed unit 1300, along with pipeline is opened, valve 1238 also is opened, and the nutrient solution band adventitious embryo H and is sent to and puts bed unit 1300.

Behind certain hour, valve 1238 cuts out, above-mentioned triple valve 1237 is opened bypass pipe 1239 simultaneously, culture fluid is discharged in the rhone 1247 by bypass pipe 1239, perhaps turn back in the concentration adjusting device, detect the adventitious embryo that the next one passes through up to second photo detector by second circulating pipe 1248.

Like this, when carrying out sorting with sorting unit, the time interval is in accordance with regulations delivered to adventitious embryo singly and puts bed unit 1300.

Put bed unit 1300 as Figure 21 (a) with (b), constitute by following several parts: to preparing adventitious embryo is put the sterilizing chamber 1310 that the incubator 1301 of bed is implemented sterilization treatment, the adventitious embryo of sending here from aforementioned sorting unit 1200 is put bed putting a chamber 1400 and send the rearmounted chamber 1500 of the incubator 1301 after putting bed under the maintenance germ-free condition in incubator 1301.Figure 21 (a) is for taking the vertical view behind the upper cover plate away, and Figure 21 (b) is for taking the front view behind the side plate away.

Sterilizing chamber 1310 has airtight sealed structure and fills water in the bottom, heater 1311 is housed in these water, because heater heating, water is evaporated into steam, make indoor 121 ℃, two atmospheric pressure that reach, in order to carrying out sterilization treatment through the incubator that netted supporting 1312 is placed on the transport platform 1313.On this transport platform 1313, hook 1314 is housed also.Herein, the 1315th, feed is advanced the entrance door of incubator, the 1316th, temperature sensor, the 1317th, pressure sensor.

Putting a chamber 1400 usefulness sends ultraviolet sterilamp 1401 and makes indoor maintenance germ-free condition.Lead to the structure of the entrance door 1402 of above-mentioned sterilizing chamber 1310, as Figure 22 (a) with (b), when strut 1403 is pushed to right-hand under cylinder 1404 effects, then bar 1405, bar 1406, bar 1407 are just through strut 1408, be pulled out by strut 1409,1410,1411 again, the retaining mechanism of hook 1405a, 1406a, 1407a is opened, and is fulcrum with hinge 1412, and door 1402 is opened.

Like this, entrance door one is opened, and chassis 1413 is just right-hand with shifting to of the steel wire rope 1415 that connects with metal card 1414, hangs up with the hook 1314 of hook 1416 with above-mentioned transport platform 1313, incubator 1301 is drawn out together with transport platform and is contained on the chassis 1413, delivers to assigned position.Steel wire rope 1415 usefulness motors 1417 drive by the pulley 1418 that rotates.Hook 1416 fixedly is also to move on pulley 1419 and 1420 mobile steel wire ropes 1421 similarly thereupon in addition.

In addition, Acetabula device 1422 is housed on the top board of putting a chamber, before putting bed, holds earlier, unload, put to give again behind the bed and cover with its lid 1301b with incubator 1301.

Put a mouth 1423 usefulness carriage 1423b and hang under the top board of putting a chamber, the adventitious embryo of being sent here singly by the sorting unit time interval is in accordance with regulations put on the culture medium carrier of bed in incubator.This front end of putting a mouth 1423 both can move on directions X along guide rail 1423a, also can be moved with the spacing of regulation on the y direction by chassis.After putting bed, just lid 1301b is sent back to assigned position.Have, 1424 is filter again, comprises filter 1425, ultraviolet lamp 1426 and fin 1427.In addition, below filter, be provided with dull and stereotypedly 1429, between it and top board carriage 1428 be housed, this plays the effect of wind shelves.

Rearmounted chamber 1500 links together with putting a chamber 1400 by entrance door 1501, use the sterilamp of forming by ultraviolet lamp 1502 to make it keep germ-free condition, similarly be loaded on the chassis 1513 together with transport platform 1313 and move it incubator with above-mentioned hook 1416.The 1524th, with 1424 filters with same structure.

The 1503rd, outlet portal, the structure of entrance door, outlet portal all is to make a lever 1504,1505 that promotes separately, door is with regard to openable form.Owing to adopt interlock, can not open simultaneously.Open outlet portal, can take out incubator.In addition, in case its structure also makes outlet portal be opened close again after, through the stipulated time, before the indoor sterilization of postposition finished, entrance door 1501 all can not be opened.

In addition, in any case arranging many slotting seedling devices in the incubator 1301, as Figure 23 (a) with (b), its structure is: disposed the gel 1319 of putting a usefulness in culture medium solution carriers 1318 such as vermiculite or perlite, will wrap around the carrier 1318 with gel 1320 simultaneously.Should insert the seedling device and also can make several one group.

So, just can at an easy rate under aseptic condition, only be put bed and easy to operate to those good adventitious embryos.

Also incubator can be rested always rearmounted indoor to its supply with discharge nutrient solution and gas is grown seedlings.

As shown in figure 24, if load onto the axial-flow type filter, just can prevent the pipeline blockage of first drainpipe 1104 in the bottom of incubator 1107.

For the mesh that prevents first and second filters 1211,1212 stops up, also can make the axial-flow type filter, the recoil loop can also be set or filter is applied mechanical oscillation.

Have again, not only can make and put a mouth and move, also can arrange some mouths of putting, accept the adventitious embryo supplied with by sorting unit successively, and unified depositing get ready, and then simultaneously each row is put bed in the x direction in the x direction.

Present embodiment is to cultivate adventitious embryo in incubator, yet as shown in figure 25, also can increase the incubator 1007 that is used for cultured calli before the flow process of adventitious embryo incubator.This method was the sorting in order to obtain good adventitious embryo, cultured calli originally, yet, because can shared sorting unit and make device obtain more efficient use.In addition, in sorting unit 1200, used triple valve 1237, but sorting and this adventitious embryo moving in culture fluid in order to guarantee adventitious embryo also can apply vibration to employed pipe, perhaps in employed pipe, electrode is set, in order to hold adventitious embryo or to make it and pipe is separated.

Be not difficult to find out according to above explanation, if use the present invention, because Zai Zhuan You can keep nutrient solution to cultivate the incubator Zhong of the culture medium carrier of base, but tear open by free-style clothing, can carry out to the coupling part the fungi-proofing connector of sterilization treatment, incubator is connected on the nutrient solution feed system and gas supply system with several nutrient tanks, just can cultivate basic Zhong Zu Zhi according to Zhi bed Yu nutrient solution and cultivate the different phase that bulk-growth is grown, realization has supply and the discharge of nutrient solution and the gas of certain ingredients under the Zai germ-free condition, thereby can low-costly realize high speed Yu seedling.

In addition, if Yong Zhi bed apparatus of the present invention, this Zhuan Zhi of You Yu have culture apparatus, according to color and/or size separation Zu Zhi cultivate the sorting unit of body, Zu Zhi that sorting is obtained cultivates the body time interval in accordance with regulations Zhi bed unit on the Zhi bed Yu culture medium singly, so realize sorting and Zhi bed and convenient operation under the Zai aseptic condition at an easy rate.

Claims (17)

1, a kind of seedling nursing device is characterized in that, this device consists of the following components:

Be equipped with the incubator of the culture medium carrier that can keep nutrient solution to cultivate base culture apparatus,

Supply with this incubator gas air supply system,

By this incubator discharge gas gas extraction system,

Be equipped with supply with this incubator nutrient solution cultivate the nutrient solution feed system of the nutrient tank of base,

By this incubator discharge the nutrient solution that has become useless cultivate the nutrient solution of base discharge system,

This incubator is connected with the discharge system with the supply of these gases and nutrient solution has the fungi-proofing connector that this connecting portion is carried out the system of sterilization treatment simultaneously, according to putting the different phase that the tissue culture bulk-growth of bed on culture medium carrier grown, implement supply and discharge that gas and nutrient solution are cultivated base.

According to the seedling nursing device of claim 1, it is characterized in that 2, above-mentioned fungi-proofing connector has following structure:

Have at least one inlet tube, the other end is open with above-mentioned incubator connection for an end of this inlet tube, at this other end first valve that can blind off a line is housed also,

Have fairlead, the one end is equipped with second flange that first flange with above-mentioned inlet tube matches, and on the pipeline section in this second flange second valve that can blind off a line is housed simultaneously, and system connectivity is supplied with, discharged to its other end with gas and nutrient solution,

Have under the approaching situation in the open end that makes these inlet tubes and fairlead, make its sealing and connecting device that forms confined space all around with the formation little clearance,

Have the bactericidal unit that the space in this obturator is carried out sterilization treatment,

Have under motionless situation that above-mentioned confined space is kept intact, make the fastener of first flange and the second flange driving fit, after the open end sterilization of bactericidal unit, make inlet tube and fairlead driving fit, connection each pipe.

3, seedling nursing device according to claim 2 is characterized in that, above-mentioned fungi-proofing connector has:

Air supply pipe,

Blast pipe,

The nutrient solution supply pipe,

The nutrient solution discharge pipe,

The front end of nutrient solution supply pipe is introduced the hydrops district that is located in the incubator, culture fluid is supplied in the incubator with realization with the gas atomization nutrient solution simultaneously.

4, seedling nursing device according to claim 2 is characterized in that, above-mentioned fungi-proofing connector has:

Air supply pipe,

Blast pipe,

The nutrient solution supply pipe,

The nutrient solution discharge pipe,

The front end of nutrient solution supply pipe is introduced the hydrops district that is located in the incubator, makes nutrient solution realize nutrient solution is supplied in the incubator by filling bubble method simultaneously.

5, according to claim 3 or 4 described seedling nursing device, it is characterized in that in above-mentioned four pipelines, having at least two to be public pipe, reversal valve is housed so that freely switch at its front end.

6, seedling nursing device according to claim 2, it is characterized in that, be provided with capillary module so that contact in the bottom of above-mentioned incubator with above-mentioned culture medium carrier and nutrient solution discharge pipe, and, also have controlled pressure and make the slightly high nutrient solution of pressure of the pressure ratio nutrient solution discharge pipe that incubator is interior discharge the promotion device.

7, seedling nursing device according to claim 1, it is characterized in that above-mentioned nutrient solution feed system has several nutrient tanks, also have the clean pipe of an aggregate supply pipe and clean aggregate supply pipe, this aggregate supply Guan Jijing valve is connected on the nutrient tank, also is connected on the fungi-proofing connector.

8, seedling nursing device according to claim 1 is characterized in that, above-mentioned nutrient solution feed system has following a few part:

Have the NaOH solution tank NaOH of several splendid attire solution the solution feedway,

The pH value regulator solution feedway of supply pH value regulator solution,

The aqua sterilisa feedway that concentration adjustment is used,

Select needed solution to mix from above-mentioned NaOH solution tank NaOH, carry out simultaneously that pH value is regulated and concentration adjustment with the mixing arrangement that obtains needed nutrient solution,

Nutrient solution feedway with several nutrient tanks, these nutrient tanks are used for storing the nutrient solution that is obtained by mixing arrangement and supply with incubator.

9, seedling nursing device according to claim 1 is characterized in that, above-mentioned culture apparatus has several incubators.

10, seedling nursing device according to claim 1 is characterized in that, above-mentioned incubator applies stimulation with wind to the tissue culture body in inside, and interior dress fan produces moving air with short its domestication.

11, a kind of bed apparatus of putting is characterized in that, this device has following a few part:

The culture apparatus that the tissue culture body is accommodated and cultivated together with culture fluid,

The sorting unit of the tissue culture body of sending here by culture apparatus according to color and/or size separation,

The tissue culture body time interval in accordance with regulations of sorting is put bed singly on medium, put bed unit.

12, the bed apparatus of putting according to claim 11, it is characterized in that, above-mentioned sorting unit comprises by first filter and the filter formed than the second little filter of screen cloth mesh of first filter, and make contain the tissue culture body culture fluid successively by and the retentate on second filter is sent to puts bed unit.

13, according to the bed apparatus of putting of claim 11 or 12, it is characterized in that: above-mentioned sorting unit has with the concentration detection apparatus of optical method detectable concentration and the result that detects according to concentration detection apparatus before reaching needed concentration supplies with the concentration adjusting device that the fluid Supplying apparatus of nutrient solution or pure water is formed.

14, the bed apparatus of putting according to claim 13, it is characterized in that, above-mentioned concentration detection apparatus has detector tube, realize concentration adjustment with above-mentioned concentration adjusting device, make and contain a tissue culture body in each quantitative culture fluid, this detector tube allows the culture fluid that contains the tissue culture body pass through by above-mentioned a certain amount of portion aly; Comprise that also the tissue culture health check-up that detects the size of tissue culture body according to the attenuation of light by this detector tube surveys device, and the tissue culture body that only will satisfy rated condition is sent to and puts bed unit.

15, the bed apparatus of putting according to claim 14 is characterized in that, above-mentioned tissue culture health check-up survey device also have detect whether the tissue culture body have a specified color distinguish the look device.

16, the bed apparatus of putting according to claim 11 is characterized in that, the above-mentioned bed unit of putting has following a few part:

To incubator carry out sterilization treatment the sterilizing chamber,

In this incubator will by the tissue culture body that above-mentioned sorting unit is sent here put successively the bed put a chamber,

The rearmounted chamber that incubator after having bactericidal unit and will putting bed takes out under germ-free condition,

And the transfer device of between each chamber, transferring incubator successively.

17, the bed apparatus of putting according to claim 16 is characterized in that, the structure of the entrance and exit of above-mentioned each chamber can not be opened it simultaneously, puts the indoor germ-free condition that keeps at any time of bed thereby make.

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