CN103733975B - Method suitable for rapid rooting of blueberry tissue culture seedlings - Google Patents
Method suitable for rapid rooting of blueberry tissue culture seedlings Download PDFInfo
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- CN103733975B CN103733975B CN201410001429.2A CN201410001429A CN103733975B CN 103733975 B CN103733975 B CN 103733975B CN 201410001429 A CN201410001429 A CN 201410001429A CN 103733975 B CN103733975 B CN 103733975B
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- cultivation cup
- culturing rack
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Abstract
The invention discloses a method suitable for rapid rooting of blueberry tissue culture seedlings. Three culture frames which are connected in series one above the other are used, and corresponding most suitable culture solutions are used at different stages and flow on a longer path, thereby increasing the yield and culture speed of the culture seedlings, and saving the using quantities of the culture solutions. In the method, a culture solution ingredient sensor is also used.
Description
Technical field
The invention belongs to field of plant tissue culture technique, be specifically related to a kind of method of suitable blueberry tissue culture seedling Rapid Rooting.
Background technology
The cultivation cycle of blueberry is often longer and can not meet the needs of time.Cultivating the cultivation speed of seedling in order to improve blueberry, the method using different culture fluid in different phase can be adopted.And cultivate in the cultivating process of seedling at large batch of blueberry, need to expend quite a large amount of culture fluids.And also do not have in prior art to cultivate seedling by fast culture blueberry, the scheme of culture fluid can be saved again.
Summary of the invention
In order to solve the problems of the technologies described above, the invention provides a kind of method of suitable blueberry tissue culture seedling Rapid Rooting, it can significantly improve the grown cultures speed of blueberry, can save again the utilization of culture fluid simultaneously.
The scheme that the present invention adopts is:
A kind of method of suitable blueberry tissue culture seedling Rapid Rooting, it uses a kind of blueberry tissue culture seedling Rapid Rooting system, described system comprises: the first culturing rack, second culturing rack, 3rd culturing rack and culture fluid allocation units, described first culturing rack, second culturing rack and the 3rd culturing rack are arranged successively up and down, described culture fluid allocation units are connected with the cultivation cup in three culturing racks by main line, wherein, cultivation cup in described first culturing rack is connected with main line by the first valve, cultivation cup in described second culturing rack is connected with main line by the second valve, cultivation cup in described 3rd culturing rack is connected with main line by the 3rd valve,
Wherein, there is in described first culturing rack the first left cultivation cup and the first right cultivation cup, described first left cultivation cup is connected with described first valve by the first left valve, described first right cultivation cup is connected with described first valve by the first right valve, the bottom of described first left cultivation cup is connected with the first left discharge pipe by the first left dump valve, is also provided with the first left culture fluid component sensor in described first left cultivation cup; The bottom of described first right cultivation cup is connected with the first right discharge pipe by the first right dump valve, is also provided with the first right culture fluid component sensor in described first right cultivation cup;
There is in described second culturing rack the second left cultivation cup and the second right cultivation cup, described second left cultivation cup is connected with described second valve by the second left valve, described second right cultivation cup is connected with described second valve by the second right valve, the bottom of described second left cultivation cup is connected with the second left discharge pipe by the second left dump valve, is also provided with the second left culture fluid component sensor in described second left cultivation cup; The bottom of described second right cultivation cup is connected with the second right discharge pipe by the second right dump valve, is also provided with the second right culture fluid component sensor in described second right cultivation cup;
There is in described 3rd culturing rack the 3rd left cultivation cup and the 3rd right cultivation cup, described 3rd left cultivation cup is connected with described 3rd valve by the 3rd left valve, described 3rd right cultivation cup is connected with described 3rd valve by the 3rd right valve, the bottom of described 3rd left cultivation cup is connected with the 3rd left discharge pipe by the 3rd left dump valve, is also provided with the 3rd left culture fluid component sensor in described 3rd left cultivation cup; The bottom of described 3rd right cultivation cup is connected with the 3rd right discharge pipe by the 3rd right dump valve, is also provided with the 3rd right culture fluid component sensor in described 3rd right cultivation cup;
Described first left discharge pipe is connected with described second left cultivation cup, and described second left discharge pipe is connected with described 3rd left cultivation cup, and described first right discharge pipe is connected with described second right cultivation cup, and described second right discharge pipe is connected with described 3rd right cultivation cup;
Described method comprises the steps:
1) first is cultivated the cultivation cup that seedling puts into described first culturing rack;
2) in the cultivation cup of described first culturing rack, the first culture fluid is injected by culture fluid allocation units;
3) after suitable culture period, the first culture fluid in the cultivation cup of described first culturing rack is discharged in the cultivation cup in described second culturing rack;
4) in the cultivation cup of the first culturing rack, the second culture fluid is injected by described culture fluid allocation units;
5) second batch is cultivated the cultivation cup that seedling puts into described second culturing rack;
6) after suitable culture period, the first culture fluid in the cultivation cup of described second culturing rack is discharged in the cultivation cup in described 3rd culturing rack;
7) the second culture fluid in the cultivation cup of described first culturing rack is discharged in the cultivation cup in described second culturing rack;
8) in the cultivation cup of described first culturing rack, the 3rd culture fluid is injected by culture fluid allocation units;
9) cultivate the 3rd batch the cultivation cup that seedling puts into described 3rd culturing rack;
10) after suitable culture period, the first culture fluid in the cultivation cup in described 3rd culturing rack is discharged;
11) the second culture fluid in the cultivation cup of described second culturing rack is discharged in the cultivation cup in described 3rd culturing rack;
12) the 3rd culture fluid in the cultivation cup of described first culturing rack is discharged in the cultivation cup in described second culturing rack; First is cultivated seedling and shifts out cultivation cup;
13) after suitable culture period, the 3rd culture fluid in the cultivation cup of described second culturing rack is discharged in the cultivation cup in described 3rd culturing rack; Second batch is cultivated seedling and is shifted out cultivation cup;
14) after suitable culture period, the 3rd culture fluid in the cultivation cup of described 3rd culturing rack is discharged; Cultivate seedling for 3rd batch and shift out cultivation cup;
In incubation, by the composition of culture fluid component sensor monitoring culture fluid, when the composition of culture fluid does not meet the demands, inject corresponding culture fluid by described culture fluid allocation units to corresponding cultivation in cup.
By such scheme, owing to employing corresponding culture fluid the most suitable in the different stages, therefore improve the cultivation speed that blueberry cultivates seedling; Meanwhile, in fact three culturing racks too increase the cultivation quantity that blueberry cultivates seedling; And three culturing racks are connected up and down, in fact make culture fluid to trickle on longer path use, make it use more abundant, save the consumption of culture fluid; Be equipped with culture fluid component sensor simultaneously, the composition quality of culture fluid is ensured.
Accompanying drawing explanation
Fig. 1 is the structural representation of cultural method of the present invention.
Embodiment
With reference to Fig. 1, a kind of method of suitable blueberry tissue culture seedling Rapid Rooting, it uses a kind of blueberry tissue culture seedling Rapid Rooting system, described system comprises: the first culturing rack 1, second culturing rack 2, 3rd culturing rack 3 and culture fluid allocation units 10, described first culturing rack 1, second culturing rack 2 and the 3rd culturing rack 3 are arranged successively up and down, described culture fluid allocation units 10 are connected with the cultivation cup in three culturing racks by main line 11, wherein, cultivation cup in described first culturing rack 1 is connected with main line 11 by the first valve 12, cultivation cup in described second culturing rack 2 is connected with main line 11 by the second valve 13, cultivation cup in described 3rd culturing rack 3 is connected with main line 11 by the 3rd valve 14,
Wherein, there is in described first culturing rack 1 first left cultivation cup 5 and the first right cultivation cup 4, described first left cultivation cup 5 is connected with described first valve 12 by the first left valve 51, described first right cultivation cup 4 is connected with described first valve 12 by the first right valve 41, the bottom of described first left cultivation cup 5 is connected with the first left discharge pipe 54 by the first left dump valve 52, is also provided with the first left culture fluid component sensor 53 in described first left cultivation cup 5; The bottom of described first right cultivation cup 4 is connected with the first right discharge pipe 44 by the first right dump valve 42, is also provided with the first right culture fluid component sensor 43 in described first right cultivation cup 4;
There is in described second culturing rack 2 second left cultivation cup 6 and the second right cultivation cup 7, described second left cultivation cup 6 is connected with described second valve 13 by the second left valve 61, described second right cultivation cup 7 is connected with described second valve 13 by the second right valve 71, the bottom of described second left cultivation cup 6 is connected with the second left discharge pipe 64 by the second left dump valve 62, is also provided with the second left culture fluid component sensor 63 in described second left cultivation cup 6; The bottom of described second right cultivation cup 7 is connected with the second right discharge pipe 74 by the second right dump valve 72, is also provided with the second right culture fluid component sensor 73 in described second right cultivation cup 7;
There is in described 3rd culturing rack 3 the 3rd left cultivation cup 8 and the 3rd right cultivation cup 9, described 3rd left cultivation cup 8 is connected with described 3rd valve 14 by the 3rd left valve 81, described 3rd right cultivation cup 9 is connected with described 3rd valve 14 by the 3rd right valve 91, the bottom of described 3rd left cultivation cup 8 is connected with the 3rd left discharge pipe 84 by the 3rd left dump valve 82, is also provided with the 3rd left culture fluid component sensor 83 in described 3rd left cultivation cup 8; The bottom of described 3rd right cultivation cup 9 is connected with the 3rd right discharge pipe 94 by the 3rd right dump valve 92, is also provided with the 3rd right culture fluid component sensor 93 in described 3rd right cultivation cup 9;
Described first left discharge pipe 54 is connected with described second left cultivation cup 6, described second left discharge pipe 64 is connected with described 3rd left cultivation cup 8, described first right discharge pipe 44 is connected with described second right cultivation cup 7, and described second right discharge pipe 74 is connected with described 3rd right cultivation cup 9;
Described method comprises the steps:
1) first is cultivated the cultivation cup that seedling puts into described first culturing rack 1;
2) in the cultivation cup of described first culturing rack 1, the first culture fluid is injected by culture fluid allocation units 10;
3) after suitable culture period, the first culture fluid in the cultivation cup of described first culturing rack 1 is discharged in the cultivation cup in described second culturing rack 2;
4) in the cultivation cup of the first culturing rack 1, the second culture fluid is injected by described culture fluid allocation units 10;
5) second batch is cultivated the cultivation cup that seedling puts into described second culturing rack 2;
6) after suitable culture period, the first culture fluid in the cultivation cup of described second culturing rack 2 is discharged in the cultivation cup in described 3rd culturing rack 3;
7) the second culture fluid in the cultivation cup of described first culturing rack 1 is discharged in the cultivation cup in described second culturing rack 2;
8) in the cultivation cup of described first culturing rack 1, the 3rd culture fluid is injected by culture fluid allocation units 10;
9) cultivate the 3rd batch the cultivation cup that seedling puts into described 3rd culturing rack;
10) after suitable culture period, the first culture fluid in the cultivation cup in described 3rd culturing rack is discharged;
11) the second culture fluid in the cultivation cup of described second culturing rack 2 is discharged in the cultivation cup in described 3rd culturing rack 3;
12) the 3rd culture fluid in the cultivation cup of described first culturing rack 1 is discharged in the cultivation cup in described second culturing rack 2; First is cultivated seedling and shifts out cultivation cup;
13) after suitable culture period, the 3rd culture fluid in the cultivation cup of described second culturing rack 2 is discharged in the cultivation cup in described 3rd culturing rack 3; Second batch is cultivated seedling and is shifted out cultivation cup;
14) after suitable culture period, the 3rd culture fluid in the cultivation cup of described 3rd culturing rack 3 is discharged; Cultivate seedling for 3rd batch and shift out cultivation cup;
In incubation, by the composition of culture fluid component sensor monitoring culture fluid, when the composition of culture fluid does not meet the demands, inject corresponding culture fluid by described culture fluid allocation units 10 to corresponding cultivation in cup.
Claims (1)
1.
a kind of method of suitable blueberry tissue culture seedling Rapid Rooting, it uses a kind of blueberry tissue culture seedling Rapid Rooting system, described system comprises: the first culturing rack (1), second culturing rack (2), 3rd culturing rack (3) and culture fluid allocation units (10), described first culturing rack (1), second culturing rack (2) and the 3rd culturing rack (3) are arranged successively up and down, described culture fluid allocation units (10) are connected with the cultivation cup in three culturing racks by main line (11), wherein, cultivation cup in described first culturing rack (1) is connected with main line (11) by the first valve (12), cultivation cup in described second culturing rack (2) is connected with main line (11) by the second valve (13), cultivation cup in described 3rd culturing rack (3) is connected with main line (11) by the 3rd valve (14),
wherein, there is in described first culturing rack (1) the first left cultivation cup (5) and the first right cultivation cup (4), described first left cultivation cup (5) is connected with described first valve (12) by the first left valve (51), described first right cultivation cup (4) is connected with described first valve (12) by the first right valve (41), the bottom of described first left cultivation cup (5) is connected with the first left discharge pipe (54) by the first left dump valve (52), is also provided with the first left culture fluid component sensor (53) in described first left cultivation cup (5); The bottom of described first right cultivation cup (4) is connected with the first right discharge pipe (44) by the first right dump valve (42), is also provided with the first right culture fluid component sensor (43) in described first right cultivation cup (4);
there is in described second culturing rack (2) the second left cultivation cup (6) and the second right cultivation cup (7), described second left cultivation cup (6) is connected with described second valve (13) by the second left valve (61), described second right cultivation cup (7) is connected with described second valve (13) by the second right valve (71), the bottom of described second left cultivation cup (6) is connected with the second left discharge pipe (64) by the second left dump valve (62), is also provided with the second left culture fluid component sensor (63) in described second left cultivation cup (6); The bottom of described second right cultivation cup (7) is connected with the second right discharge pipe (74) by the second right dump valve (72), is also provided with the second right culture fluid component sensor (73) in described second right cultivation cup (7);
there is in described 3rd culturing rack (3) the 3rd left cultivation cup (8) and the 3rd right cultivation cup (9), described 3rd left cultivation cup (8) is connected with described 3rd valve (14) by the 3rd left valve (81), described 3rd right cultivation cup (9) is connected with described 3rd valve (14) by the 3rd right valve (91), the bottom of described 3rd left cultivation cup (8) is connected with the 3rd left discharge pipe (84) by the 3rd left dump valve (82), is also provided with the 3rd left culture fluid component sensor (83) in described 3rd left cultivation cup (8); The bottom of described 3rd right cultivation cup (9) is connected with the 3rd right discharge pipe (94) by the 3rd right dump valve (92), is also provided with the 3rd right culture fluid component sensor (93) in described 3rd right cultivation cup (9);
described first left discharge pipe (54) is connected with described second left cultivation cup (6), described second left discharge pipe (64) is connected with described 3rd left cultivation cup (8), described first right discharge pipe (44) is connected with described second right cultivation cup (7), and described second right discharge pipe (74) is connected with described 3rd right cultivation cup (9);
described method comprises the steps:
1) first is cultivated the cultivation cup that seedling puts into described first culturing rack (1);
2) in the cultivation cup of described first culturing rack (1), the first culture fluid is injected by culture fluid allocation units (10);
3) after suitable culture period, the first culture fluid in the cultivation cup of described first culturing rack (1) is discharged in the cultivation cup in described second culturing rack (2);
4) in the cultivation cup of the first culturing rack (1), the second culture fluid is injected by described culture fluid allocation units (10);
5) second batch is cultivated the cultivation cup that seedling puts into described second culturing rack (2);
6) after suitable culture period, the first culture fluid in the cultivation cup of described second culturing rack (2) is discharged in the cultivation cup in described 3rd culturing rack (3);
7) the second culture fluid in the cultivation cup of described first culturing rack (1) is discharged in the cultivation cup in described second culturing rack (2);
8) in the cultivation cup of described first culturing rack (1), the 3rd culture fluid is injected by culture fluid allocation units (10);
9) cultivate the 3rd batch the cultivation cup that seedling puts into described 3rd culturing rack (3);
10) after suitable culture period, the first culture fluid in the cultivation cup in described 3rd culturing rack (3) is discharged;
11) the second culture fluid in the cultivation cup of described second culturing rack (2) is discharged in the cultivation cup in described 3rd culturing rack (3);
12) the 3rd culture fluid in the cultivation cup of described first culturing rack (1) is discharged in the cultivation cup in described second culturing rack (2); First is cultivated seedling and shifts out cultivation cup;
13) after suitable culture period, the 3rd culture fluid in the cultivation cup of described second culturing rack (2) is discharged in the cultivation cup in described 3rd culturing rack (3); Second batch is cultivated seedling and is shifted out cultivation cup;
14) after suitable culture period, the 3rd culture fluid in the cultivation cup of described 3rd culturing rack (3) is discharged; Cultivate seedling for 3rd batch and shift out cultivation cup;
in incubation, by the composition of culture fluid component sensor monitoring culture fluid, when the composition of culture fluid does not meet the demands, inject corresponding culture fluid by described culture fluid allocation units (10) to corresponding cultivation in cup.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410001429.2A CN103733975B (en) | 2014-01-03 | 2014-01-03 | Method suitable for rapid rooting of blueberry tissue culture seedlings |
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| CN201410001429.2A CN103733975B (en) | 2014-01-03 | 2014-01-03 | Method suitable for rapid rooting of blueberry tissue culture seedlings |
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| CN103733975B true CN103733975B (en) | 2015-02-04 |
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Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6474936A (en) * | 1987-09-14 | 1989-03-20 | Sumitomo Cement Co | Culture through pebble by multi-stage bed and device therefor |
| JP2002101757A (en) * | 2000-09-27 | 2002-04-09 | Hokuriku Electric Power Co Inc:The | Method for culturing plant and plant culture device used for the method |
| JP2006280252A (en) * | 2005-03-31 | 2006-10-19 | Koken Boring Mach Co Ltd | Underground agricultural factory system |
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2014
- 2014-01-03 CN CN201410001429.2A patent/CN103733975B/en not_active Expired - Fee Related
Patent Citations (5)
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|---|---|---|---|---|
| US4729188A (en) * | 1986-12-09 | 1988-03-08 | Cheng Ming Kwei | Automatic sprouts culture bag |
| CN1143893A (en) * | 1994-03-11 | 1997-02-26 | 株式会社诚和 | Multistage plant culture method and multistage plant culture apparatus for use therein |
| CN201230501Y (en) * | 2008-07-17 | 2009-05-06 | 蒲志理 | Water-ploughing circulation cultivation system |
| CN201682856U (en) * | 2010-02-24 | 2010-12-29 | 丽莱登实业有限公司 | Base frame device for water plow homework |
| CN102577921A (en) * | 2011-01-10 | 2012-07-18 | 太仓戈林农业科技有限公司 | Soilless culture device |
Non-Patent Citations (3)
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| JP特开2006-280252A 2006.10.19 * |
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