CN103719024A - Microorganism culture method for enhancing blue-green algae ingestion capacity of planktonic microorganisms - Google Patents
Microorganism culture method for enhancing blue-green algae ingestion capacity of planktonic microorganisms Download PDFInfo
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Abstract
The invention discloses a microorganism culture method for enhancing blue-green algae ingestion capacity of planktonic microorganisms. The microorganism culture method includes the following specific steps that culture pools are built, fertilizer is applied to the culture pools, and then eutrophic pool water is obtained; composite algae culture solution is added to the eutrophic pool water; the temperature of the pool water of all the culture pools reaches 8-20 DEG C, and the planktonic microorganisms are transferred in the first culture pool; the planktonic microorganisms cultured in the first culture pool are transferred to the second culture pool, and the planktonic microorganisms cultured in the second culture pool are transferred to the next culture pool in the same way until the planktonic microorganisms high in blue-green algae ingestion capacity are obtained. The planktonic microorganisms high in blue-green algae ingestion capacity are domesticated through the similarly polluted eutrophic pool water and the special composite algae culture solution, and the planktonic microorganisms further have the advantages of being high in fertility and good in adaptability to the freshwater environment.
Description
Technical field
The present invention relates to microorganism field, particularly a kind of ingest microbial culture method of blue-green alge ability of plankton that strengthens.
Background technology
Why blue-green alge is broken out, because the mankind's various activities in production, to river, the water body discharge nitrogen such as lake, phosphorus nutrition material, in the situation that lake ecological self-purification capacity is destroyed, nitrogen, the constantly accumulation in a large number in water body of the nutriments such as phosphorus, causes unicellular alga, particularly blue-green algae (being mainly microcystic aeruginosa) is overflowed, the result of severe contamination water quality.
Cells of Blue-green Algae has very flourishing phycocolloid layer mostly, and by the thick polysaccharose substance of one deck, is surrounded outside cell, and these phycocolloid and polysaccharose substance almost can not be decomposed by the digestive ferment of any higher mammal.Therefore, the appearance of blue-green algae biology, has almost become the cecum of food chain and biological chain.The expert of domestic and international many environmental protection and biological aspect adopts higher mammal to comprise that fish improvement blue-green algae pollutes, and does not all obtain desirable result.
Undoubtedly, Xiang Shuizhong adds chemical herbicide and algae flocculant to kill very soon, precipitation algae.Chemical treatment is the solution that solves algae pollution most convenient, but on ecology, this is the worst method, because chemicals and their catabolite can finally run up in human body by biological chain.Importantly, chemical treatment can not be eliminated this basic problem of excessive fertilization, once chemicals is decomposed, dilutes, and the amount reproduction of algae will occur again, needs continual chemical treatment.
When dissolved oxygen is high, phosphate usually crystallization forms undissolved chemical combination state; And when dissolved oxygen is on the low side, will there is a large amount of dissolvings in phosphate.Therefore, the method for blowing aeration can reduce phosphatic solvability and greatly suppress algae reproduction.In the process of biotechnology, water oxygenation is very useful.But single oxygenation can not solve the too much problem of the original nutrient content of water body, the length of nutrition causes algal tufa still inevitable apart from recycle.
Microorganism formulation can be improved the microorganism in water body and substrate mud, and carries out benign decomposition and the mineralising of organic matter and nutritive element, and some nutritive elements are temporarily settled out with inertia form, and its result is similar to the method for blowing aeration.It should be noted that, itself is just decided by culture environment the existence of microorganism, and requiring to have the population of absolute predominance, general microbial treatment can only keep the good working state of 15～30 days, once condition changes, (comprises weather, substrate, various water quality conditions are as dissolved oxygen, acid-base value, temperature), these microorganism formulations must constantly add, otherwise the outburst of polluting may be more serious.
Algae is nutritious, decomposes rapidly, can be made into good organic manure.But it is too little to salvage the required mesh of algae, and water content is too high again, and from the viewpoint of business, the expense of salvaging is higher than the value of organic manure.
As everyone knows, plant waterborne (as weter hyacinth, water peanut, water lettuce, also comprise vegetables waterborne, flowers waterborne etc.) really can absorb the nutrient in water body, but they block the water surface, hide sunlight, cause biology at the bottom of part aquatic organism and mud, the particularly death of submerged plant, causes a large amount of dissolvings of nutritive salt in bed mud then, and their drawback is far longer than the benefit that they bring.
Summary of the invention
The object of the present invention is to provide a kind of ingest microbial culture method of blue-green alge ability of plankton that strengthens, the method can improve the ingest ability of algae of plankton.
For achieving the above object, the invention provides following technical scheme:
Strengthen the ingest microbial culture method of blue-green alge ability of plankton, concrete steps are as follows:
(1) build culture pond, the area of culture pond is 100～300 square metres, and the degree of depth is 80～120 centimetres, and soil property is shale or argillo arenaceous, at the bottom of pond and pool wall non-leakage; The first disinfection of culture pond, then add 40～60 centimetres of fresh water after sterilization to culture pond; To culture pond fertilising, obtain eutrophication Chi Shui;
The fertilizer that described fertilising adopts is fertilizer and inorganic fertilizer, and inorganic fertilizer is 2～8 grams/cubic metre of superphosphate, 15～20 grams/cubic metre, urea; Fertilizer is 100～300 grams/cubic metre of animal wastes, first fermentation process before fertilizer is used;
(2) in the water of eutrophication pond, add compound algae culture liquid; The consumption of described compound algae culture liquid is 3～6 liters/m; After 1～3 day, water colour is by clear thin out green or light brown;
Described compound algae culture liquid and preparation method thereof is: select and clean blue-green algae and green alga, and drop into leaching liquor, then broken, the clear liquid filtering, is inoculated into clear liquid in container and inflates cultivation, and the culture fluid in container is through disinfecting, adopt continuous culture method, at intensity of illumination 5000～6000Lx, cultivate 2～4 days under 20～28 degrees Celsius, shake container every day 2～6 times; When the total cellular score of blue-green algae and green alga exceedes 10
6during CFU/ml, obtain compound algae culture liquid;
In container, the component of every liter of culture fluid is: 50 milligrams, ammonium nitrate, 1 milligram of potassium dihydrogen phosphate, 5 milligrams of ironic citrates, 3 milligrams, calcium chloride, 3 milligrams, iron chloride, 5 milligrams, sodium chloride, 2 milliliters of animal wastes leachates, 998 milliliters of distilled water;
(3) described culture pond quantity is 2～6; Each culture pond all does the processing of step (1) and step (2);
(4) make all culture ponds pond water water temperature reach 8～20 ℃, to first culture pond inoculation plankton; The larger plankton of screening volume, inoculate in second culture pond, screen again the plankton that volume is larger, be inoculated in next culture pond, repeat above-mentioned steps until obtain ingesting blue-green algae and the strong plankton of green alga ability, the inoculum density of described plankton is 5～15/liter;
(5) plankton step (4) being obtained is inoculated into large cultivation in the large culture pond of 1000～5000 square metres, and before inoculation, large culture pond does the processing of step (1) and step (2); Also to large culture pond, drop into complex micro organism fungicide, brown sugar, soya-bean milk and wheat bran; The total number of bacteria of complex micro organism fungicide is not less than 5 × 10
9pic/g, injected volume is 1～5ppm; Complex micro organism fungicide comprises photosynthetic bacteria, nitrification and denitrification bacterium, bud pole bacterium, actinomycetes, saccharomycete and lactic acid bacteria, brown sugar 0.05～0.1 kilograms per cubic meter, dregs of beans 1～2 kilograms per cubic meter, wheat bran 1～2 kilograms per cubic meter.
As further scheme, what in step (1), culture pond disinfection adopted is bleaching powder, before bleaching powder is used, detects chlorinty, and the bleaching powder consumption of chlorinty 30% is 25～30 grams/m, and drug effect disappears for 3 days～5 days.
As further scheme, step (1) adopts repeatedly fertilising, applies fertilizer afterwards before midday.
As further scheme, step (2) adds water along with the breeding water colour of blue-green algae and green alga is deepened early stage in good time; Material recycle in later stage pond is accelerated, and constantly output plankton, makes Trace Element Imbalance in water body, therefore needs to supplement the fresh water after sterilization.
As further scheme, described plankton is wheel animalcule, cladocera.
Beneficial effect: the eutrophication Chi Shui by similar pollution of the present invention and special compound algae culture liquid have been tamed out a kind of ingest blue-green algae and the strong plankton of green alga ability, and it is strong that it also has fertility, adapts to the good advantage of fresh water environment.
Embodiment
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described, obviously, described embodiment is only the present invention's part embodiment, rather than whole embodiment.Based on the embodiment in the present invention, those of ordinary skills, not making the every other embodiment obtaining under creative work prerequisite, belong to the scope of protection of the invention.
Embodiment 1
In the embodiment of the present invention, a kind of ingest microbial culture method of blue-green alge ability of plankton that strengthens, concrete steps are as follows:
(1) build culture pond, the area of culture pond is 100 square metres, and the degree of depth is 80 centimetres, and soil property is shale or argillo arenaceous, at the bottom of pond and pool wall non-leakage; The first disinfection of culture pond, then add 40 centimetres of fresh water after sterilization to culture pond; To culture pond fertilising, obtain eutrophication Chi Shui;
The fertilizer that described fertilising adopts is fertilizer and inorganic fertilizer, and inorganic fertilizer is 2 grams/cubic metre of superphosphate, 15 grams/cubic metre, urea; Fertilizer is 100 grams/cubic metre of animal wastes, first fermentation process before fertilizer is used;
(2) in the water of eutrophication pond, add compound algae culture liquid; The consumption of described compound algae culture liquid is 6 liters/m; After 1 day, water colour is by clear thin out green or light brown;
Described compound algae culture liquid and preparation method thereof is: select and clean blue-green algae and green alga, and drop into leaching liquor, then broken, the clear liquid filtering, is inoculated into clear liquid in container and inflates cultivation, and the culture fluid in container is through disinfecting, adopt continuous culture method, at intensity of illumination 5000Lx, cultivate 4 days under 20 degrees Celsius, shake container every day 6 times; When the total cellular score of blue-green algae and green alga exceedes 10
6during CFU/ml, obtain compound algae culture liquid;
In container, the component of every liter of culture fluid is: 50 milligrams, ammonium nitrate, 1 milligram of potassium dihydrogen phosphate, 5 milligrams of ironic citrates, 3 milligrams, calcium chloride, 3 milligrams, iron chloride, 5 milligrams, sodium chloride, 2 milliliters of animal wastes leachates, 998 milliliters of distilled water;
(3) described culture pond quantity is 2～6; Each culture pond all does the processing of step (1) and step (2);
(4) make all culture ponds pond water water temperature reach 8～20 ℃, to first culture pond inoculation plankton; The larger plankton of screening volume, inoculate in second culture pond, screen again the plankton that volume is larger, be inoculated in next culture pond, repeat above-mentioned steps until obtain ingesting blue-green algae and the strong plankton of green alga ability, the inoculum density of described plankton is 5～15/liter;
(5) plankton step (4) being obtained is inoculated into large cultivation in the large culture pond of 1000 square metres, and before inoculation, large culture pond does the processing of step (1) and step (2); Also to large culture pond, drop into complex micro organism fungicide, brown sugar, soya-bean milk and wheat bran; The total number of bacteria of complex micro organism fungicide is not less than 5 × 10
9pic/g, injected volume is 2ppm; Complex micro organism fungicide comprises photosynthetic bacteria, nitrification and denitrification bacterium, bud pole bacterium, actinomycetes, saccharomycete and lactic acid bacteria, brown sugar 0.05 kilograms per cubic meter, dregs of beans 1 kilograms per cubic meter, wheat bran 1 kilograms per cubic meter.
As further scheme, what in step (1), culture pond disinfection adopted is bleaching powder, before bleaching powder is used, detects chlorinty, and the bleaching powder consumption of chlorinty 30% is 25 grams/m, and drug effect disappears for 3 days.
As further scheme, step (1) adopts repeatedly fertilising, applies fertilizer afterwards before midday.
As further scheme, step (2) adds water along with the breeding water colour of blue-green algae and green alga is deepened early stage in good time; Material recycle in later stage pond is accelerated, and constantly output plankton, makes Trace Element Imbalance in water body, therefore needs to supplement the fresh water after sterilization.
As further scheme, described plankton is wheel animalcule, cladocera.
Embodiment 2
In the embodiment of the present invention, a kind of ingest microbial culture method of blue-green alge ability of plankton that strengthens, concrete steps are as follows:
(1) build culture pond, the area of culture pond is 200 square metres, and the degree of depth is 100 centimetres, and soil property is shale or argillo arenaceous, at the bottom of pond and pool wall non-leakage; The first disinfection of culture pond, then add 50 centimetres of fresh water after sterilization to culture pond; To culture pond fertilising, obtain eutrophication Chi Shui;
The fertilizer that described fertilising adopts is fertilizer and inorganic fertilizer, and inorganic fertilizer is 5 grams/cubic metre of superphosphate, 18 grams/cubic metre, urea; Fertilizer is 200 grams/cubic metre of animal wastes, first fermentation process before fertilizer is used;
(2) in the water of eutrophication pond, add compound algae culture liquid; The consumption of described compound algae culture liquid is 5 liters/m; After 1 day, water colour is by clear thin out green or light brown;
Described compound algae culture liquid and preparation method thereof is: select and clean blue-green algae and green alga, and drop into leaching liquor, then broken, the clear liquid filtering, is inoculated into clear liquid in container and inflates cultivation, and the culture fluid in container is through disinfecting, adopt continuous culture method, at intensity of illumination 5600Lx, cultivate 3 days under 25 degrees Celsius, shake container every day 3 times; When the total cellular score of blue-green algae and green alga exceedes 10
6during CFU/ml, obtain compound algae culture liquid;
In container, the component of every liter of culture fluid is: 50 milligrams, ammonium nitrate, 1 milligram of potassium dihydrogen phosphate, 5 milligrams of ironic citrates, 3 milligrams, calcium chloride, 3 milligrams, iron chloride, 5 milligrams, sodium chloride, 2 milliliters of animal wastes leachates, 998 milliliters of distilled water;
(3) described culture pond quantity is 2～6; Each culture pond all does the processing of step (1) and step (2);
(4) make all culture ponds pond water water temperature reach 8～20 ℃, to first culture pond inoculation plankton; The larger plankton of screening volume, inoculate in second culture pond, screen again the plankton that volume is larger, be inoculated in next culture pond, repeat above-mentioned steps until obtain ingesting blue-green algae and the strong plankton of green alga ability, the inoculum density of described plankton is 5～15/liter;
(5) plankton step (4) being obtained is inoculated into large cultivation in the large culture pond of 3000 square metres, and before inoculation, large culture pond does the processing of step (1) and step (2); Also to large culture pond, drop into complex micro organism fungicide, brown sugar, soya-bean milk and wheat bran; The total number of bacteria of complex micro organism fungicide is not less than 5 × 10
9pic/g, injected volume is 3ppm; Complex micro organism fungicide comprises photosynthetic bacteria, nitrification and denitrification bacterium, bud pole bacterium, actinomycetes, saccharomycete and lactic acid bacteria, brown sugar 0.1 kilograms per cubic meter, dregs of beans 1.5 kilograms per cubic meter, wheat bran 1.5 kilograms per cubic meter.
As further scheme, what in step (1), culture pond disinfection adopted is bleaching powder, before bleaching powder is used, detects chlorinty, and the bleaching powder consumption of chlorinty 30% is 30 grams/m, and drug effect disappears for 5 days.
As further scheme, step (1) adopts repeatedly fertilising, applies fertilizer afterwards before midday.
As further scheme, step (2) adds water along with the breeding water colour of blue-green algae and green alga is deepened early stage in good time; Material recycle in later stage pond is accelerated, and constantly output plankton, makes Trace Element Imbalance in water body, therefore needs to supplement the fresh water after sterilization.
As further scheme, described plankton is wheel animalcule, cladocera.
Embodiment 3
In the embodiment of the present invention, a kind of ingest microbial culture method of blue-green alge ability of plankton that strengthens, concrete steps are as follows:
(1) build culture pond, the area of culture pond is 300 square metres, and the degree of depth is 120 centimetres, and soil property is shale or argillo arenaceous, at the bottom of pond and pool wall non-leakage; The first disinfection of culture pond, then add 60 centimetres of fresh water after sterilization to culture pond; To culture pond fertilising, obtain eutrophication Chi Shui;
The fertilizer that described fertilising adopts is fertilizer and inorganic fertilizer, and inorganic fertilizer is 8 grams/cubic metre of superphosphate, 20 grams/cubic metre, urea; Fertilizer is 300 grams/cubic metre of animal wastes, first fermentation process before fertilizer is used;
(2) in the water of eutrophication pond, add compound algae culture liquid; The consumption of described compound algae culture liquid is 3 liters/m; After 2 days, water colour is by clear thin out green or light brown;
Described compound algae culture liquid and preparation method thereof is: select and clean blue-green algae and green alga, and drop into leaching liquor, then broken, the clear liquid filtering, is inoculated into clear liquid in container and inflates cultivation, and the culture fluid in container is through disinfecting, adopt continuous culture method, at intensity of illumination 6000Lx, cultivate 3 days under 25 degrees Celsius, shake container every day 3 times; When the total cellular score of blue-green algae and green alga exceedes 10
6during CFU/ml, obtain compound algae culture liquid;
In container, the component of every liter of culture fluid is: 50 milligrams, ammonium nitrate, 1 milligram of potassium dihydrogen phosphate, 5 milligrams of ironic citrates, 3 milligrams, calcium chloride, 3 milligrams, iron chloride, 5 milligrams, sodium chloride, 2 milliliters of animal wastes leachates, 998 milliliters of distilled water;
(3) described culture pond quantity is 2～6; Each culture pond all does the processing of step (1) and step (2);
(4) make all culture ponds pond water water temperature reach 8～20 ℃, to first culture pond inoculation plankton; The larger plankton of screening volume, inoculate in second culture pond, screen again the plankton that volume is larger, be inoculated in next culture pond, repeat above-mentioned steps until obtain ingesting blue-green algae and the strong plankton of green alga ability, the inoculum density of described plankton is 5～15/liter;
(5) plankton step (4) being obtained is inoculated into large cultivation in the large culture pond of 1000～5000 square metres, and before inoculation, large culture pond does the processing of step (1) and step (2); Also to large culture pond, drop into complex micro organism fungicide, brown sugar, soya-bean milk and wheat bran; The total number of bacteria of complex micro organism fungicide is not less than 5 × 10
9pic/g, injected volume is 5ppm; Complex micro organism fungicide comprises photosynthetic bacteria, nitrification and denitrification bacterium, bud pole bacterium, actinomycetes, saccharomycete and lactic acid bacteria, brown sugar 0.1 kilograms per cubic meter, dregs of beans 2 kilograms per cubic meter, wheat bran 2 kilograms per cubic meter.
As further scheme, what in step (1), culture pond disinfection adopted is bleaching powder, before bleaching powder is used, detects chlorinty, and the bleaching powder consumption of chlorinty 30% is 30 grams/m, and drug effect disappears for 5 days.
As further scheme, step (1) adopts repeatedly fertilising, applies fertilizer afterwards before midday.
As further scheme, step (2) adds water along with the breeding water colour of blue-green algae and green alga is deepened early stage in good time; Material recycle in later stage pond is accelerated, and constantly output plankton, makes Trace Element Imbalance in water body, therefore needs to supplement the fresh water after sterilization.
As further scheme, described plankton is wheel animalcule, cladocera.
To those skilled in the art, obviously the invention is not restricted to the details of above-mentioned example embodiment, and in the situation that not deviating from spirit of the present invention or essential characteristic, can realize the present invention with other concrete form.Therefore, no matter from which point, all should regard embodiment as exemplary, and be nonrestrictive, scope of the present invention is limited by claims rather than above-mentioned explanation, is therefore intended to all changes that drop in the implication and the scope that are equal to important document of claim to include in the present invention.
In addition, be to be understood that, although this specification is described according to embodiment, but be not that each embodiment only comprises an independently technical scheme, this narrating mode of specification is only for clarity sake, those skilled in the art should make specification as a whole, and the technical scheme in each embodiment also can, through appropriately combined, form other embodiments that it will be appreciated by those skilled in the art that.
Claims (5)
1. strengthen the ingest microbial culture method of blue-green alge ability of plankton, it is characterized in that, concrete steps are as follows:
Build culture pond, the area of culture pond is 100～300 square metres, and the degree of depth is 80～120 centimetres, and soil property is shale or argillo arenaceous, at the bottom of pond and pool wall non-leakage; The first disinfection of culture pond, then add 40～60 centimetres of fresh water after sterilization to culture pond; To culture pond fertilising, obtain eutrophication Chi Shui;
The fertilizer that described fertilising adopts is fertilizer and inorganic fertilizer, and inorganic fertilizer is 2～8 grams/cubic metre of superphosphate, 15～20 grams/cubic metre, urea; Fertilizer is 100～300 grams/cubic metre of animal wastes, first fermentation process before fertilizer is used;
In the water of eutrophication pond, add compound algae culture liquid; The consumption of described compound algae culture liquid is 3～6 liters/m; After 1～3 day, water colour is by clear thin out green or light brown;
Described compound algae culture liquid and preparation method thereof is: select and clean blue-green algae and green alga, and drop into leaching liquor, then broken, the clear liquid filtering, is inoculated into clear liquid in container and inflates cultivation, and the culture fluid in container is through disinfecting, adopt continuous culture method, at intensity of illumination 5000～6000Lx, cultivate 2～4 days under 20～28 degrees Celsius, shake container every day 2～6 times; When the total cellular score of blue-green algae and green alga exceedes 10
6during CFU/ml, obtain compound algae culture liquid;
In container, the component of every liter of culture fluid is: 50 milligrams, ammonium nitrate, 1 milligram of potassium dihydrogen phosphate, 5 milligrams of ironic citrates, 3 milligrams, calcium chloride, 3 milligrams, iron chloride, 5 milligrams, sodium chloride, 2 milliliters of animal wastes leachates, 998 milliliters of distilled water;
Described culture pond quantity is 2～6; Each culture pond all does the processing of step (1) and step (2);
Make all culture ponds pond water water temperature reach 8～20 ℃, to first culture pond inoculation plankton; The larger plankton of screening volume, inoculate in second culture pond, screen again the plankton that volume is larger, be inoculated in next culture pond, repeat above-mentioned steps until obtain ingesting blue-green algae and the strong plankton of green alga ability, the inoculum density of described plankton is 5～15/liter;
The plankton that step (4) is obtained is inoculated into large cultivation in the large culture pond of 1000～5000 square metres, and before inoculation, large culture pond does the processing of step (1) and step (2); Also to large culture pond, drop into complex micro organism fungicide, brown sugar, soya-bean milk and wheat bran; The total number of bacteria of complex micro organism fungicide is not less than 5 × 10
9pic/g, injected volume is 1～5ppm; Complex micro organism fungicide comprises photosynthetic bacteria, nitrification and denitrification bacterium, bud pole bacterium, actinomycetes, saccharomycete and lactic acid bacteria, brown sugar 0.05～0.1 kilograms per cubic meter, dregs of beans 1～2 kilograms per cubic meter, wheat bran 1～2 kilograms per cubic meter.
2. the enhancing according to claim 1 plankton microbial culture method of blue-green alge ability of ingesting, it is characterized in that, what in step (1), culture pond disinfection adopted is bleaching powder, before being used, bleaching powder detects chlorinty, the bleaching powder consumption of chlorinty 30% is 25～30 grams/m, and drug effect disappears for 3 days～5 days.
3. the enhancing according to claim 1 plankton microbial culture method of blue-green alge ability of ingesting, is characterized in that, step (1) adopts repeatedly fertilising, applies fertilizer afterwards before midday.
4. the enhancing according to claim 1 plankton microbial culture method of blue-green alge ability of ingesting, is characterized in that, step (2) adds water along with the breeding water colour of blue-green algae and green alga is deepened early stage in good time; Material recycle in later stage pond is accelerated, and constantly output plankton, makes Trace Element Imbalance in water body, therefore needs to supplement the fresh water after sterilization.
5. the enhancing according to claim 1 plankton microbial culture method of blue-green alge ability of ingesting, is characterized in that, described plankton is wheel animalcule, cladocera.
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