CN103718960B

CN103718960B - The breeding method of tree grape fast results

Info

Publication number: CN103718960B
Application number: CN201310696936.8A
Authority: CN
Inventors: 陈财宝
Original assignee: Liuzhou Tianzi Horticulture Co Ltd
Current assignee: Liuzhou Tianzi Horticulture Co Ltd
Priority date: 2013-12-18
Filing date: 2013-12-18
Publication date: 2016-02-10
Anticipated expiration: 2033-12-18
Also published as: CN103718960A

Abstract

The invention discloses the breeding method of tree grape fast results, adopt sponge liquid medium first Multiplying culture tree grape squamous subculture material, switching is cultured to liquid nutrient medium again, repeatedly be forwarded to culture of rootage in the solid culture medium being added with agar powder afterwards to fall asleep, namely obtain setting Tissue culture the seedling of grape.Beneficial effect of the present invention is: the breeding method of tree grape fast results provided by the invention, has the following advantages: 1, set grape group training material and fully can absorb nutrition, improve quality and output, the rate of increase can reach 5-6 doubly; 2, the tree grape adopting the inventive method to cultivate, has within 6 years, be reduced to 4 years by growth cycle, and ties 4 fruits in 1 year, substantially increases fruiting rate; 2, sponge can reuse, and liquid nutrient medium packing preparation is more convenient, and the apparatus such as bottle easily clean up; 3, save the expense buying agar powder, reduce production cost.

Description

The breeding method of tree grape fast results

Technical field

The present invention relates to the fast numerous field of plant tissue culture seedling, be specifically related to the breeding method setting grape fast results.

Background technology

Garbo fruit (Pliniacauliflora (Mart.) Kausel) belongs to Myrtaceae evergreen shrubs, because fruit shapes is like grape, therefore also known as tree grape.It grows up slowly, and bark is light grey, comes off, leave light tone speckle after coming off in flake.Leaf is to life, and petiole is short, has fine hair, blade keratin, and bottle green is glossy, lanceolar or ellipse.Its flowers are born on trunk and major branch, spend little, white, and stamen is most.

At present, tree grape mostly is introduces Taiwan 2 season fruit by Brazil, result after 6 years, and 1 year result 2 times, fruiting rate is extremely low.

Summary of the invention

Object of the present invention is exactly for above-mentioned defect of the prior art, provides a kind of breeding method that can make tree grape fast results.

To achieve these goals, technical scheme provided by the invention is: the breeding method of tree grape fast results, adopt sponge liquid medium first Multiplying culture tree grape squamous subculture material, switching is cultured to liquid nutrient medium again, repeatedly be forwarded to culture of rootage in the solid culture medium being added with agar powder afterwards to fall asleep, namely obtain setting Tissue culture the seedling of grape.

Further, the breeding method of above-mentioned tree grape fast results, comprises the following steps:

1) sponge liquid medium is prepared: select columniform high temperature resistant filtration sponge, in the same size bottom diameter and ventilating cover thread bottle, sponge thickness is 1cm, on sponge, evenly have 15 bores is 0.8cm, the degree of depth is the V-shape hole of 0.5cm size, sponge block is lain in bottom blake bottle, one of perforate faces up, direct running water prepare medicines and through constant volume mix up pH value liquid nutrient medium, be dispensed in right amount and be placed with in the blake bottle of sponge, nutrient solution has just been invaded and finishes sponge block; Described liquid nutrient medium is 1/2MS medium;

2) sterilizing of medium: point sponge liquid medium installed step 1) obtained is through autoclaving, and sterilising temp is 121-123 DEG C, and sterilization pressure is 0.11-0.13MP, and sterilization time is 22-25 minute, obtains sterilizing wild Oryza species;

3) inoculation of culture materials: under germ-free condition, tree grape culture materials is cut into simple bud and inserts respectively in the V-shape hole of planting the sponge liquid medium after sterilizing, carry out fast breeding cultivation, incubation time is 30-35 days, and the rate of increase is 5-6 times;

4) switching of propagation material: the material after propagation in step 3) is held under the arm out to cut to inoculate in fresh liquid nutrient medium and proceeded Multiplying culture; After this, when cultivating 30-35 days, again material being carried out cutting changing in fresh liquid nutrient medium and carrying out Multiplying culture;

5) culture of rootage: after the tree grape propagation material after Multiplying culture step 4) obtained is transferred to some, cut into individual plant to be transferred in the solid culture medium being added with agar powder and to carry out culture of rootage, after 2 ~ 3 months cultivate hardening, namely obtain tree Tissue culture the seedling of grape transplant strain.

Beneficial effect of the present invention is: the breeding method of tree grape fast results provided by the invention, has the following advantages:

1, set grape group training material and fully can absorb nutrition, improve quality and output, the rate of increase can reach 5-6 doubly;

2, the tree grape adopting the inventive method to cultivate, has within 6 years, be reduced to 4 years by growth cycle, and ties 4 fruits in 1 year, substantially increases fruiting rate;

2, sponge can reuse, and liquid nutrient medium packing preparation is more convenient, and the apparatus such as bottle easily clean up;

3, save the expense buying agar powder, reduce production cost.

Embodiment

embodiment 1:

(1) making of sponge liquid medium:

Select columniform high temperature resistant filtration sponge, in the same size bottom diameter and ventilating cover thread bottle, sponge thickness is 1cm, on sponge, evenly have 15 bores is 0.8cm, the degree of depth is the V-shape hole of 0.5cm size, is lain in by sponge block bottom blake bottle, and one of perforate faces up.Because not adding agar powder, can direct running water prepare medicines and through constant volume mix up pH value liquid nutrient medium, be finally dispensed in right amount and be placed with in the blake bottle of sponge, nutrient solution has just been invaded and finishes sponge block;

(2) sterilizing of medium:

Because the sponge selected is exotic material, main component is polyurethane, have anticorrosive, have no side effect, high temperature resistant (can reach 200 DEG C), intensity high (resilience is good), water imbibition be extremely strong, the advantages such as recoverable, after repeatedly autoclaving, modification is still not reusable; Divide the medium installed through autoclaving (sterilising temp is 121-123 degree, and pressure is 0.11-0.13MP, and the time is 22-25 minute), finally the liquid nutrient medium that sterilizing is good is put into medium storeroom for subsequent use;

(3) inoculation of culture materials:

Culture materials cuts into simple bud by aseptic working platform and inserts kind respectively in the V-shape hole of sponge liquid medium, because sponge has good resilience and water imbibition, there are certain fixation and moisture-keeping functions to the culture materials be inserted in V-shape hole, are beneficial to the growth of culture and the absorption of nutrient;

(4) Multiplying culture of culture materials:

Cultivate the material on sponge liquid medium, due to liquid nutrient medium Middle molecule or ion motion very fast, its base portion fully can touch nutrient solution and can absorb nutrient preferably, also the quinones harmful substance that self otch is secreted is diluted in liquid nutrient medium simultaneously, reduce the generation of brownization, thus reach the effect of fast breeding, generally cultivate the rate of increase after 30-35 days and can reach 5-6 doubly;

(5) switching of propagation material:

After cultivating 30-35 days in propagation material liquid medium within, culture materials is proliferate constantly, has absorbed most nutrition and moisture, also have accumulated the harmful components such as certain quinones in liquid nutrient medium simultaneously; Now the material after propagation should be held under the arm out to cut to inoculate in fresh liquid nutrient medium and proceed Multiplying culture, after this when cultivating 30-35 days, constantly material is carried out cutting changing in fresh liquid nutrient medium and carry out Multiplying culture, production can be made to reach the material of some and scale;

(6) culture of rootage:

After tree grape propagation material is transferred to the quantity needed for production, is cut into individual plant and be transferred in the solid culture medium being added with agar powder and carry out culture of rootage, can be transplanted by bottle outlet after 2 ~ 3 months cultivate hardening.

Last it is noted that the foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although with reference to previous embodiment to invention has been detailed description, for a person skilled in the art, it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.

Claims

1. set the breeding method of grape fast results, it is characterized in that, comprise the following steps:

1) sponge liquid medium is prepared: select columniform high temperature resistant filtration sponge, in the same size bottom diameter and ventilating cover thread bottle, sponge thickness is 1cm, on sponge, evenly have 15 bores is 0.8cm, and the degree of depth is the V-shape hole of 0.5cm size, is lain in by sponge block bottom blake bottle, one of perforate faces up, direct running water prepare medicines and through constant volume mix up pH value liquid nutrient medium, be dispensed in right amount and be placed with in the blake bottle of sponge, make nutrient solution just soak sponge block; Described liquid nutrient medium is 1/2MS medium;