CN1037061C - Method for preparing edible pollen - Google Patents

Abstract

The present invention relates to a method for preparing edible pollen, which belongs to the field of nutrient health-care foods. The method comprises the steps: bee pollen is added to enzymes to be fermented; the fermented mixture is dried, pulverized and soaked with acid alcohol to be dissolved, and then the dissolved mixture is centrifugally separated; supernatant fluid is decompressed or concentrated with sucking air to prepare pollen nutrient fluid; precipitates are dried, pulverized and sieved to prepare pollen nutrient powder. The powder does not have allergy sources, abnormal smell, adipocere, stimulation or side effect and is easy to be digested, and furthermore, nutrients are easy absorbed by human bodies. The pollen nutrient fluid and the nutrient powder are good health-care products for people. The method for preparing edible pollen has the advantages of simple technology, easy popularization and application, and obvious social and economic benefits.

Description

The new method that edible pollen is made

The invention belongs to the method that nutraceutical is made, outstanding tool is the new method of edible pollen manufacturing technology.

The melissa powder is the mixture of plant spermatid and the special secretion of honeybee.It contains many bodies requisite material of surviving.Existing known melissa powder contains 20 several amino acids, 30 several kinds of mineral elements, 10 multivitamins, tens kinds of organized enzymes, multiple antibiotic and glucides.So pollen is referred to as " miniature nutrition library ".China's medicine monograph Shennong's Herbal has the earliest been put down in writing " the health key medicine " of pollen as body-building, beauty treatment, longevity.Known to the clinical data record, pollen has good protective action to cardiovascular system, has the function that strengthens capillary intensity, can prevent the generation of patients with coronary heart disease headstroke.Pollen can promote the growth of brain cell, and the function of the refreshing smart system of enhancing maincenter has significant curative effect to insomnia, psychasthenia disease.Therefore the researcher is more.Pollen product through looking into present development and market sale is single mechanical breaking-wall method or breaking wall by fermentation.Because exposore has hard sporopollenin and cellulose, so sporoderm-broken rate is not high.Breaking wall by fermentation also can only make exposore produce germinal aperature and discharge some nutritions.The percentage of damage of these two kinds of methods is all below 90%, and the edible pollen of producing is all more coarse, and what have has peculiar smell and a sensitization source, the adipocere matter that can not digest in addition that has, even have also have toxicity, thereby influenced the nutritional utilization and the absorption of pollen.

The objective of the invention is in order to overcome the deficiencies in the prior art part, the spy provides a kind of pollen grain is added enzyme fermentation, uses Mechanical Crushing again, leaches a kind of integrated approach of nutrient solution and nutrient powder at last.This method can greatly improve the pollen percentage of damage, and can eliminate the peculiar smell of pollens nutrition thing, and anaphylactogen and stodgy adipocere matter make the absorption that is fully utilized of pollens nutrition material.

The objective of the invention is to realize by following measure:

Choose fresh energetic melissa powder (preferably purity is at the pure pollen more than 80%) and get a little pollen then on slide, drip 0.5% triphenyltetrazolium chloride on pollen, covered, putting into 35 ℃ of water-baths keeps taking out after 15 minutes, examine under a microscope color and judge its activity, redness is active strong; Pale red, it is colourless to take second place, and does not have activity.With energetic pollen with the impurity elimination of sieving of 18 order/inches, with 75 degree edible ethanol sterilizations.Pollen after the impurity elimination sterilization adds 10% brewer yeast liquid (compound method of S.cerevisiae brewer yeast: add 10 milliliters of brewer yeasts in 90 ml distilled waters), add 0.2% cellulase again, 0.2% pectase (compound method of cellulase and pectase: add the pectase that adds 0.2 milligram in 0.2 milligram cellulase 100 ml distilled waters in 100 ml distilled waters) and pol reach the pollen glues of 12 degree, and (compound method of pollen glue: the pure honey more than 60 degree adds 8 parts of water purification for 1 part, in 65 ℃ water-bath, change honey 15 minutes, being cooled to 40 then can use when spending, consumption is 1: 1, promptly 50 grams, thousand pollen add 50 gram pollen glues), pollen water content is reached about 28%, abundant mixing, put into the fermentation dish, thickness is about 2 centimetres, fermentation is 48 hours in 37 ℃ of constant incubators, can smell the sugared fragrance of pollen, just remove anaphylactogen.Melissa powder after the fermentation is dried under 45 ℃ hot blast, need one hour approximately, pulverize with micronizer the cooling back, powder adds 10 times the sour alcohol infiltration dissolving (compound method of the sour alcohol that infiltration is for the first time used: add 40 milliliters of pure absolute ethyl alcohols of analysis in 60 ml distilled waters, transfer PH to reach 4 with 0.2% citric acid more then; The collocation method of citric acid solution: add 0.2 gram citric acid in 100 ml distilled waters) stirring 3 hours rapidly below 50 ℃, filter with the sieve of 160 order/inches the cooling back, filtrate was with centrifugal 15 minutes of 2000 rev/mins of centrifuges, ladle out the adipocere matter on centrifuge tube upper strata with the spoon spoon, pour out supernatant, be placed on below 25 ℃ standby.Sediment add again 6 times be different from for the first time the sour alcohol of concentration and soak into dissolving (the sour alcohol compound method of using: add 20 milliliters in 80 ml distilled waters and analyze pure absolute ethyl alcohols, PH is 4 with 0.2% citric acid solution accent) for the second time.Carry out extracting the second time supernatant according to primary method.The supernatant of twice gained is lumped together, and it is standby to be placed on preservation below 25 ℃.The sediment of getting supernatant carries out suction filtration again, dries under 45 ℃ hot blast, and pulverize with common pulverizer the cooling back, sieves with 80 order/inches, and the fine powder that obtains can be made food additive, and meal can be made concentrated feed.The supernatant after taking out, add four pull-up fat filtered through gauze and thoroughly remove adipocere matter with 160 orders/inch sieve again.Bleed with vacuum kettle then or concentrate and remove vinosity and peculiar smell, just obtain to concentrate pollen nutrient liquid with 30-50 millimetres of mercury decompression.

Embodiment:

Choose fresh energetic melissa powder (purity is at the pure pollen more than 80%), get a little pollen then on slide, dripping 0.5% triphenyltetrazolium chloride mixes with pollen, covered, put into 35 ℃ of water-baths and kept 15 minutes, examine under a microscope its color after the taking-up, redness is active strong, pale red takes second place, and colourless do not have an activity.Get the energetic melissa powder of 50 grams, with the impurity elimination of sieving of 18 order/inches, spend 50 milliliters of sprinklings of edible ethanols pollen with 75, turn over while spraying, sterilization carries out disinfection.Add 5 milliliters of brewer yeast liquid again, cellulase solution 0.1 gram, pectase liquid 0.1 gram and pol reach 12 degree, change 50 milliliters of pollen glues that are cooled to 40 ℃ behind the honey through 65 ℃, and fully mixing reaches about 28% pollen water content, and promptly hand is pinched agglomeratingly, and loosing of touching is to spend.Put into the fermentation dish, thickness is about 2 centimetres, and fermentation is 48 hours in 37 ℃ of constant incubators, smells sugared fragrance and has just removed anaphylactogen.After the taking-up, dry under 45 ℃ hot blast, need one hour approximately, pulverize with micronizer the cooling back, and percentage of damage reaches more than 98%.It is that 40% sour alcohol soaks into dissolving for 500 milliliters that pollen end after the fragmentation adds concentration, using magnetic stirrer 3 hours below 50 ℃, filter with the sieve of 160 order/inches the cooling back, filtrate was with centrifugal 15 minutes of 2000 rev/mins of high schemings, ladle out the adipocere matter of centrifuge tube upper strata white with the spoon spoon, the supernatant of pouring out is continued to employ.It is that 20% sour alcohol soaks into dissolving for 300 milliliters that sediment after centrifugal adds concentration again.Carry out extracting the second time supernatant according to the method described above.The supernatant of twice gained is lumped together, be placed on below 25 ℃ and preserve.Remaining sediment carries out suction filtration, dries about one hour under 45 ℃ hot blast.Pulverize with common pulverizer at last, sieve, just can get fine powder 30 grams and make food additive, make concentrated feed about meal 5 grams with 80 order/inches.Take out supernatant, add four pull-up fat filtered through gauze and thoroughly remove adipocere matter with 160 orders/inch sieve.Be concentrated to about 30 degree of refraction pol through 45 ℃ of vacuum 30-50 millimetres of mercury decompressions, just removed vinosity and peculiar smell, obtain 200 milliliters pollen concentrated nutrient solution at last, add 0.2 gram ascorbic acid, can preserve half a year below 25 ℃.The low more preservation time of temperature is long more.

The present invention has following characteristics:

1. small investment instant effect of the present invention, convenient operation is implemented.

2. this method reaches more than 98% the percentage of damage of pollen, whole technological process all be Operate below 50 ℃, the nutritional labeling of pollen self is not destroyed, nutritional labeling Very abundant, nutritive value is high.

3. concentrate method such as bleed by fermentation, pulverizing, infiltration, stirring, centrifugal and vacuum, removed anaphylactogen, peculiar smell and vinosity, therefore food taste purity, nonirritant and the side effect of making.

4. removed adipocere matter, more helped body digesting and assimilating nutritional labeling.

5. the pollen concentrate and the nutrient powder of gained of the present invention can be used in the nutraceutical field of health care.

Claims (1)

1. method that edible pollen is made is to carry out Mechanical Crushing or the pollens nutrition agent is produced in the fragmentation of fermenting with the melissa powder, it is characterized in that:

(1) mix covered with a little melissa powder on 0.5% triphenyltetrazolium chloride and the slide, put into 35 ℃ of water-baths and protected special 15 minutes, after the taking-up, examine under a microscope color and judge its activity, red, active strong; Pale red, take second place; Colourless, there is not activity;

(2) with energetic melissa powder 50 grams, with the impurity elimination of sieving of 18 order/inches, with 75 degree edible ethanol (50 milliliters) sterilizations, add 5 milliliters of 10% brewer yeast (S.cer-evisiae) liquid (compound method of brewer yeast: add 10 gram brewer yeasts) then with 90 ml distilled waters, add 0.2% cellulase solution 0.1 gram again, 0.2% pectase liquid 0.1 gram, (the compound method of cellulase and pectase liquid: with the cellulase of 0.2 milligram of 100 ml distilled waters adding, 100 ml distilled waters add 0.2 milligram pectase) and pol reach 50 milliliters of the 12 pollen glues of spending, (the compound method of pollen glue: add 8 parts of water purification for 1 part with the above pure honey of 60 degree, in 65 ℃ water-bath, change honey 15 minutes, being cooled to 40 can use when spending, consumption is 1: 1, promptly 50 gram dried flower powder add 50 gram pollen glues), pollen water content is reached about 28%, abundant mixing, put into the fermentation dish, thickness is about 2 centimetres, fermentation is 48 hours in 37 ℃ of constant incubators, can smell the sugared fragrance of pollen, just remove anaphylactogen;

(3) the melissa powder after the fermentation is dried under 45 ℃ hot blast, need one hour approximately, pulverize with micronizer the cooling back, then, it is that 4 concentration is that 40% sour alcohol (500 milliliters) soaks into the dissolving (compound method of sour alcohol: add 40 milliliters of pure absolute ethyl alcohols of analysis with 60 ml distilled waters that pollen end adds 10 times pH value, transfer PH to reach 4 with 0.2% citric acid solution again, the compound method of citric acid solution: add 0.2 gram citric acid) with 100 ml distilled waters, stirring 3 hours rapidly below 50 ℃, filter with the sieve of 160 order/inches the cooling back, filtrate was with centrifugal 15 minutes of 2000 rev/mins of centrifuges, ladle out the adipocere matter on centrifuge tube upper strata with the spoon spoon, pour out supernatant, it is standby to be placed on preservation below 25 ℃, it is that 4 concentration is that 20% sour alcohol (300 milliliters) soaks into the dissolving (compound method of sour alcohol: add 20 milliliters of pure absolute ethyl alcohols of analysis with 80 ml distilled waters that sediment adds 6 times PH again, transferring PH with 0.2% citric acid is 4), carry out extracting the second time supernatant according to primary method, the supernatant of twice gained is lumped together;

(4) sediment after centrifugal is dried under 45 ℃ hot blast, and about one hour, pulverized with common pulverizer the cooling back, sieves with 80 order/inches, just gets the pollens nutrition agent;

(5) supernatant of twice gained is lumped together, add four pull-up fat filtered through gauze with 160 orders/inch sieve again, thoroughly remove adipocere matter, filtrate is bled with vacuum kettle or is concentrated with the decompression of 30-50 millimetres of mercury, removes vinosity and peculiar smell, just obtains to concentrate pollen nutrient liquid.