CN101095947B - Multilevel bionic melissa powder and the method for preparing the same - Google Patents
Multilevel bionic melissa powder and the method for preparing the same Download PDFInfo
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- CN101095947B CN101095947B CN2007100182315A CN200710018231A CN101095947B CN 101095947 B CN101095947 B CN 101095947B CN 2007100182315 A CN2007100182315 A CN 2007100182315A CN 200710018231 A CN200710018231 A CN 200710018231A CN 101095947 B CN101095947 B CN 101095947B
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Abstract
The invention relates to a method for producing multi-stage bionic bee pollen and its preparation. The method for producing bionic bee pollen comprises following steps: picking bee pollen, disinfecting, desensitizing, that is called first-stage treatment; treating comb bee pollen liquid, that is second-stage treatment; treating yeast liquid, that is third stage treatment; treating lactic acid bacteria liquid, that is fourth stage treatment; producing particle and drying, disinfecting, and producing capsule, or soft capsule, tablet or bee grease. The invention also relates to health-care preparation taking said bionic bee pollen as raw material and the production method. The bee pollen is converted stage by stage to make the active enzyme, organic peptide, amino acid and other component to be similar to bee pollen in comb. The invention optimizes the bee pollen preparation process and provides a safe and fast way for bee comb pollen production preparation.
Description
Technical field
The present invention relates to the preparation method of the extraction and preparation technique technical field, particularly a kind of multilevel bionic melissa powder of pollen, the present invention also comprises the preparation and the preparation method of the bee pollen preparation that makes with this method.
Background technology
By biology of bee discovered that Nidus Vespae pollen is only the real food of Apis.If the bee colony midium or long term has lacked pollen, the unsound phenomenons of growth such as wing will appear not having in the Apis offspring, so bee pollen is an indispensable food in the Apis vital movement.The mankind take the history of the existing more than one thousand years of bee pollen, but to the research of Nidus Vespae pollen just since modern age.Bee pollen product on the market mostly is in raw material level level, fails to reach the level of Nidus Vespae pollen, and this is because the production process of bee pollen is artificial obtaining with trap from the beehive doorway, and pollen is brought in the inherent lair of beehive without Apis and brewageed; And being Apis, Nidus Vespae pollen brewages the food that forms after in lair, constantly calling in Mel, self saliva, between the two difference that Here it is.Nidus Vespae pollen has certain adhesion with the lair wall in lair, and the scope that distributes on Nidus Vespae is very little, can not mass production Nidus Vespae pollen in the mode of present production Lac regis apis or Mel.From prior art, producing the bee pollen product has and adopts yeast fermentation, also has to adopt lactic acid bacteria fermentation, and this all is the single-stage bionics techniques, and bee pollen is transformed effectively.
Summary of the invention
Technical problem to be solved by this invention is to adopt the multilevel bionic technology, finishes bee pollen step by step and transforms, and makes composition such as the organized enzyme in the pollen, organic peptide, aminoacid and Nidus Vespae pollen approaching, and the preparation method of a kind of multilevel bionic melissa powder and preparation thereof is provided.
Technical problem of the present invention solves by following method:
A kind of preparation method of multilevel bionic melissa powder prepares by following processing step:
A. the collection of raw material bee pollen, sterilization: manually take bee pollen, selected remove impurity sterilizes under normal temperature and pressure conditions, makes the raw material bee pollen reach the food hygiene index request;
B. desensitization is handled: press 15～30% of raw material bee pollen weight and spray normal saline, the back that stirs is to cultivate 48～72 hours under 30～38 ℃ of conditions in temperature, finishes the first order and handles;
C. the Nidus Vespae pollen liquid is handled: to finishing the bee pollen that the first order is handled, by the even Nidus Vespae pollen culture fluid that sprays 200～300ml of every part of 1KG, be to cultivate 48～72 hours under 30～35 ℃ of conditions in temperature, finish the second level and handle;
D. yeast liquid is handled: to finishing the bee pollen that handle the second level, evenly spraying 200～300ml yeast liquid by every part, is to cultivate 48～72 hours under 30～38 ℃ of conditions in temperature, finishes third level processing;
E. lactobacillus suspension is handled: to finishing the bee pollen that the third level is handled, evenly spraying 200～300ml lactobacillus suspension by every kg, is to cultivate 48～72 hours under 28～35 ℃ of conditions in temperature, finishes the fourth stage and handles;
F. granulate and oven dry: finish the bee pollen that the fourth stage is handled, every part of 1kg evenly adds the pepsin tablet powder of 9 120 units, make granule with 60 purpose gauzes after, dry processing, day temperature is controlled between 55～65 ℃, and stirs once every 2 hours; Nocturnal temperature is controlled between 40～45 ℃, and continuously baking makes in the pollen water content reach 3～5% and gets final product;
G. sterilization: the bee pollen that will finish oven dry places the ozone sterilization jar to sterilize, and sterilizing time is 20～30 minutes, finishes in the plastic bag of packing into after the sterilization standby;
The preparation method of Nidus Vespae pollen liquid is among the described step c:
C.1. manually from the Nidus Vespae Nidus Vespae, gather Nidus Vespae pollen;
C.2. in distilled water, add Mel, regulate its pol between 18～24%;
C.3. with Nidus Vespae pollen with in 5%～10% Mel solution that joins c.2, stir, the solution pH value is adjusted between 3.5～5;
C.4. be to cultivate under 35～38 ℃ of conditions to get final product in 48～72 hours in temperature.
The preparation method of yeast liquid is in the described steps d:
Measure distilled water 400ml, add 1/10～1/15 sterilization bee pollen, regulate its pol between 18～24% with Mel, the adjusting pH value carries out natural fermentation under 25～38 ℃ of conditions between 3.5～5, cultivate to get final product in 7～15 days.
The preparation method of lactobacillus suspension is among the described step e:
Measure distilled water 400ml, adding 1/10～1/15 sterilization bee pollen is measured pulp-water dish juice 100ml and is joined in the pollen liquid, carries out natural fermentation under 22～32 ℃ of conditions of temperature, cultivates to get final product in 5～7 days.
The dosage form of described multilevel bionic melissa powder comprises:
A. capsule or soft capsule preparation adopt 100% bionic melissa powder;
B. tablet adopts 80～85% bionic melissa powder, adds starch 15～20%;
C. sweet unguentum: batching adopts concentration ° Be42 Mel 50～60%, bionic melissa powder 40～50%.
The present invention also comprises the health food of the bionic melissa powder preparation that makes with said method, makes by following raw material and prescription:
Bionic melissa powder 50～60% is equipped with 40～50% Radix Angelicae Sinensis fine powder, or Radix Astragali fine powder, or the Radix Ginseng fine powder, and pharmaceutical methods is made tablet or capsule routinely.
Described health food, make by following raw material and prescription:
Bionic melissa powder 50%, royal jelly lyophilized powder 25%, propolis powder 25%, pharmaceutical methods is made tablet or capsule routinely.
The present invention comprises that also the bionic melissa powder that makes with said method is the oral liquid of preparation of raw material, makes as follows:
A. take by weighing 1 part of 1kg of bionic melissa powder that claim 1 makes, be dissolved in the ethanol liquid of 2L concentration 30～50%, extract pollen liquid;
B. take by weighing Radix Angelicae Sinensis moiety 0.5kg, cutting is with the ethanol extraction angelica fluid of 2L concentration 30～50%;
C. pollen liquid and angelica fluid are pressed 3: 2 mixed, add the Mel of 1/2～1/4 concentration ° Be42 in a mixed liquor, make the flower of Radix Angelicae Sinensis powder oral liquid.
Radix Angelicae Sinensis described in the above-mentioned oral liquid can be replaced with the Radix Astragali or Radix Ginseng.
The present invention makes bee pollen finish conversion step by step, makes compositions such as the organized enzyme in the pollen, organic peptide, aminoacid and Nidus Vespae pollen approaching.In the course of processing of bee pollen, finished a whole set of optimum organization, created an approach efficiently for the mankind take the Nidus Vespae pollen product.
The specific embodiment
Embodiment 1
1, the collection of raw material bee pollen, sterilization: manually take bee pollen 1kg, selected remove impurity sterilizes under normal temperature and pressure conditions, makes the raw material bee pollen reach the food hygiene index request.
2, desensitization is handled: spray the 200ml normal saline, the back that stirs is to cultivate 72 hours under 30 ℃ of conditions in temperature, finishes the first order and handles.
3, the Nidus Vespae pollen liquid is handled:
At first prepare the Nidus Vespae pollen liquid:
(1) manually from the Nidus Vespae Nidus Vespae, gathers Nidus Vespae pollen;
(2) in distilled water, add Mel, regulate its pol 18%;
(3) Nidus Vespae pollen is joined in the Mel solution of (2) with 5%, stir, the solution pH value is adjusted in 3.5;
(4) be to cultivate under 35 ℃ of conditions to get final product in 48 hours in temperature.
To finishing the bee pollen that the first order is handled, every kg evenly sprays the Nidus Vespae pollen culture fluid of 200ml, is to cultivate 48 hours under 30 ℃ of conditions in temperature, finishes the second level and handles.
4, yeast liquid is handled:
The preparation of yeast liquid:
Measure distilled water 400ml, add 1/15 sterilization bee pollen, regulate its pol 18% with Mel, the adjusting pH value carries out natural fermentation 3.5 under 25 ℃ of conditions, cultivate to get final product in 15 days.
To finishing the bee pollen that handle the second level, evenly spray the 200ml yeast liquid by every part, be that 30 ℃ of conditions were cultivated 72 hours in temperature, finish third level processing.
5, lactobacillus suspension is handled:
The preparation of lactobacillus suspension:
Measure distilled water 400ml, adding 1/15 sterilization bee pollen is measured pulp-water dish juice 100ml and is joined in the pollen liquid, carries out natural fermentation under 22 ℃ of conditions of temperature, cultivates to get final product in 7 days.
The pulp-water dish juice that the present invention adopts is to be popular in a kind of traditional lactic acid fermented drink of the sweet peaceful band in Shan, northwest, and manufacture method is:
Herba Apii graveolentis or wild vegetable are cut into chunks, boil in boiling water about 3 minutes, pull the dish section out and put into vessel, other adds boiled water, adds the wheat flour that accounts for gross weight about 1% in addition and sticks with paste, and after stirring, adds a cover, and natural fermentation under room temperature condition more than 22 ℃ got final product after 7 days.
To finishing the bee pollen that the third level is handled, evenly spray the 200ml lactobacillus suspension by every kg, be to cultivate 72 hours under 28 ℃ of conditions in temperature, finish the fourth stage and handle.
6, granulate and oven dry: finish the bee pollen that the fourth stage is handled, 1kg evenly adds the pepsin tablet powder of 9 120 units, make granule with 60 purpose gauzes after, dry processing, day temperature is controlled at 55 ℃, and every stirring in 2 hours once; Nocturnal temperature is controlled at 40 ℃, and continuously baking makes in the pollen water content equal 3 and gets final product.
7, sterilization: the bee pollen that will finish oven dry places the ozone sterilization jar to sterilize once more, and sterilizing time is 25 minutes, finishes in the plastic bag of packing into after the sterilization standby.
8, adopt 100% above-mentioned bionic melissa powder, make the capsule or the soft capsule preparation of all size.
Embodiment 2
1, the collection of raw material bee pollen, sterilization: manually take 1 bee pollen, selected remove impurity sterilizes under normal temperature and pressure conditions, makes the raw material bee pollen reach the food hygiene index request;
2, desensitization is handled: press 20% of raw material bee pollen weight and spray normal saline, the back that stirs is to cultivate 48 hours under 38 ℃ of conditions in temperature, finishes the first order and handles;
3, the Nidus Vespae pollen liquid is handled:
At first prepare the Nidus Vespae pollen liquid:
(1) manually from the Nidus Vespae Nidus Vespae, gathers Nidus Vespae pollen;
(2) in distilled water, add Mel, regulate its pol 24%;
(3) with Nidus Vespae pollen with in the 10% Mel solution that joins d.2, stir, the solution pH value is adjusted to 5;
(4) be to cultivate under 38 ℃ of conditions to get final product in 72 hours in temperature.
To finishing the bee pollen that the first order is handled, 1kg evenly sprays the Nidus Vespae pollen culture fluid of 300ml, is to cultivate 72 hours under 35 ℃ of conditions in temperature, finishes the second level and handles;
4, yeast liquid is handled:
The preparation of yeast liquid:
Measure distilled water 400ml, add 1/10 sterilization bee pollen, regulate its pol 24% with Mel, the adjusting pH value carries out natural fermentation 5 under 38 ℃ of conditions, cultivate to get final product in 7 days.
To finishing the bee pollen that handle the second level, evenly spray the 300ml yeast liquid by every part, be to cultivate 48 hours under 38 ℃ of conditions in temperature, finish third level processing.
5, lactobacillus suspension is handled:
The preparation of lactobacillus suspension:
Measure distilled water 400ml, adding 1/10 sterilization bee pollen is measured pulp-water dish juice 100ml and is joined in the pollen liquid, carries out natural fermentation under 32 ℃ of conditions of temperature, cultivates to get final product in 5 days.
To finishing the bee pollen that the third level is handled, evenly spray the 300ml lactobacillus suspension by every kg, be to cultivate 48 hours under 35 ℃ of conditions in temperature, finish the fourth stage and handle.
6, granulate and oven dry: finish the bee pollen that the fourth stage is handled, 1kg evenly adds the pepsin tablet powder of 9 120 units, make granule with 60 purpose gauzes after, dry processing, day temperature is controlled at 65 ℃, and every stirring in 2 hours once; Nocturnal temperature is controlled at 45 ℃, and continuously baking makes in the pollen water content equal 5 and gets final product.
7, sterilization: the bee pollen that will finish oven dry places the ozone sterilization jar to sterilize once more, and sterilizing time is 25 minutes, finishes in the plastic bag of packing into after the sterilization standby;
8, get the 800g bionic melissa powder, add 200g starch, make 4000 according to a conventional method, every 0.25 tablet.Or the Mel 300g of employing concentration ° Be42, bionic melissa powder 200g makes 1 bottle of 500g honey unguentum according to a conventional method.
Embodiment 3: health food
Get bionic melissa powder 50kg, Radix Angelicae Sinensis fine powder 50kg, or Radix Astragali fine powder, or Radix Ginseng fine powder, pharmaceutical methods is made Radix Angelicae Sinensis pollen tablet routinely.
Embodiment 4
Get bionic melissa powder 60kg, Radix Astragali fine powder 40kg, pharmaceutical methods is made the astragalus farina tablet routinely.
Embodiment 5
Get bionic melissa powder 50kg, royal jelly lyophilized powder 25kg, propolis powder 25kg, pharmaceutical methods is made honeybee three health-care capsules routinely.
Embodiment 6: oral liquid
1. multilevel bionic melissa powder 1kg is dissolved in the ethanol liquid of 2L concentration 30%, extracts pollen liquid;
B. take by weighing Radix Angelicae Sinensis 0.5kg, cutting is with the ethanol extraction angelica fluid of 2L concentration 30%;
C. pollen liquid and angelica fluid are pressed 3: 2 mixed, add the Mel of 1/2 concentration ° Be42 in a mixed liquor, make the flower of Radix Angelicae Sinensis powder oral liquid.
Embodiment 7
1. multilevel bionic melissa powder 1kg is dissolved in the ethanol liquid of 2L concentration 50%, extracts pollen liquid;
B. take by weighing Radix Angelicae Sinensis 0.5kg, cutting is with the ethanol extraction angelica fluid of 2L concentration 50%;
C. pollen liquid and angelica fluid are pressed 3: 2 mixed, and the Mel of the concentration ° Be42 of adding 1/4 is made the flower of Radix Angelicae Sinensis powder oral liquid in a mixed liquor.
Claims (8)
1. the preparation method of a multilevel bionic melissa powder is characterized in that by following processing step preparation:
A. the collection of raw material bee pollen, sterilization: manually take bee pollen, selected remove impurity sterilizes under normal temperature and pressure conditions, makes the raw material bee pollen reach the food hygiene index request;
B. desensitization is handled: press 15～30% of raw material bee pollen weight and spray normal saline, the back that stirs is to cultivate 48～72 hours under 30～38 ℃ of conditions in temperature, finishes the first order and handles;
C. the Nidus Vespae pollen liquid is handled: to finishing the bee pollen that the first order is handled, by the even Nidus Vespae pollen culture fluid that sprays 200～300ml of every part of 1kg, be to cultivate 48～72 hours under 30～35 ℃ of conditions in temperature, finish the second level and handle;
D. yeast liquid is handled: to finishing the bee pollen that handle the second level, evenly spraying 200～300ml yeast liquid by every part, is to cultivate 48～72 hours under 30～38 ℃ of conditions in temperature, finishes third level processing;
E. lactobacillus suspension is handled: to finishing the bee pollen that the third level is handled, evenly spraying 200～300ml lactobacillus suspension by every KG, is to cultivate 48～72 hours under 28～35 ℃ of conditions in temperature, finishes the fourth stage and handles;
F. granulate and oven dry: finish the bee pollen that the fourth stage is handled, every part of 1kg evenly adds the pepsin tablet powder of 9 120 units, make granule with 60 purpose gauzes after, dry processing, day temperature is controlled between 55～65 ℃, and stirs once every 2 hours; Nocturnal temperature is controlled between 40～45 ℃, and continuously baking makes in the pollen water content reach 3～5% and gets final product;
G. sterilization: the bee pollen that will finish oven dry places the ozone sterilization jar to sterilize, and sterilizing time is 20～30 minutes, finishes in the plastic bag of packing into after the sterilization standby;
The preparation method of Nidus Vespae pollen liquid is among the above-mentioned steps c:
C.1. manually from the Nidus Vespae Nidus Vespae, gather Nidus Vespae pollen;
C.2. in distilled water, add Mel, regulate its pol between 18～24%;
C.3. with Nidus Vespae pollen with in 5%～10% Mel solution that joins c.2, stir, the solution pH value is adjusted between 3.5～5;
C.4. be to cultivate under 35～38 ℃ of conditions to get final product in 48～72 hours in temperature.
2. the preparation method of multilevel bionic melissa powder according to claim 1 is characterized in that the preparation method of yeast liquid in the described steps d is:
Measure distilled water 400ml, add 1/10～1/15 sterilization bee pollen, regulate its pol between 18～24% with Mel, the adjusting pH value carries out natural fermentation under 25～38 ℃ of conditions between 3.5～5, cultivate to get final product in 7～15 days.
3. the preparation method of multilevel bionic melissa powder according to claim 1 is characterized in that the preparation method of lactobacillus suspension among the described step e is:
Measure distilled water 400ml, adding 1/10～1/15 sterilization bee pollen is measured pulp-water dish juice 100ml and is joined in the pollen liquid, carries out natural fermentation under 22～32 ℃ of conditions of temperature, cultivates to get final product in 5～7 days;
Described pulp-water dish juice be popular in Shan, northwest sweet peaceful one the band a kind of traditional lactic acid fermented drink, its manufacture method is:
Herba Apii graveolentis or wild vegetable are cut into chunks, boil in boiling water about 3 minutes, pull the dish section out and put into vessel, add boiled water, add the wheat flour that accounts for gross weight 1% in addition and stick with paste, after stirring, add a cover, natural fermentation under room temperature condition more than 22 ℃ got final product after 7 days.
4. the preparation method of multilevel bionic melissa powder according to claim 1 is characterized in that the dosage form of described multilevel bionic melissa powder comprises:
A. capsule or soft capsule preparation adopt 100% bionic melissa powder;
B. tablet adopts 80～85% bionic melissa powder, adds starch 15～20%;
C. sweet unguentum: batching adopts concentration ° Be42 Mel 50～60%, bionic melissa powder 40～50%.
5. the health food of a bee pollen preparation that makes with the preparation method of the described multilevel bionic melissa powder of claim 1 is characterized in that making by following raw material and prescription:
Bionic melissa powder 50～60% is equipped with 40～50% Radix Angelicae Sinensis fine powder, or Radix Astragali fine powder, or the Radix Ginseng fine powder, and pharmaceutical methods is made tablet or capsule routinely.
6. the health food of a bee pollen preparation that makes with the preparation method of the described multilevel bionic melissa powder of claim 1 is characterized in that making by following raw material and prescription:
Bionic melissa powder 50%, royal jelly lyophilized powder 25%, propolis powder 25%, pharmaceutical methods is made tablet or capsule routinely.
7. the oral liquid of a bee pollen preparation that makes with the preparation method of the described multilevel bionic melissa powder of claim 1 is characterized in that making by the following method:
A. take by weighing 1 part of 1kg of bionic melissa powder that preparation method according to claim 1 makes, be dissolved in the ethanol liquid of 2L concentration 30～50%, extract pollen liquid;
B. take by weighing Radix Angelicae Sinensis moiety 0.5kg, cutting is with the ethanol extraction angelica fluid of 2L concentration 30～50%;
C. pollen liquid and angelica fluid are pressed 3: 2 mixed, add the Mel of 1/2～1/4 concentration ° Be42 in a mixed liquor, make the flower of Radix Angelicae Sinensis powder oral liquid.
8. the oral liquid of a kind of bee pollen preparation that makes with the preparation method of the described multilevel bionic melissa powder of claim 1 according to claim 7 is characterized in that described Radix Angelicae Sinensis replaces with the Radix Astragali or Radix Ginseng.
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| CN2007100182315A CN101095947B (en) | 2007-07-11 | 2007-07-11 | Multilevel bionic melissa powder and the method for preparing the same |
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| CN2007100182315A CN101095947B (en) | 2007-07-11 | 2007-07-11 | Multilevel bionic melissa powder and the method for preparing the same |
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| CN101095947B true CN101095947B (en) | 2010-11-03 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101999569A (en) * | 2010-10-19 | 2011-04-06 | 吉林修正药业新药开发有限公司 | Health food for dispelling chloasma |
| CN102784171A (en) * | 2012-08-08 | 2012-11-21 | 杨茂森 | Preparation method of freeze-drying activated bionic honeycomb pollen and preparation thereof |
| CN107373555A (en) * | 2017-06-22 | 2017-11-24 | 杨佳林 | A kind of natural honey Bee Pollen joint product and preparation method thereof |
| CN109170902A (en) * | 2018-11-07 | 2019-01-11 | 中国科学院兰州化学物理研究所 | A kind of fructus lycii Bee Pollen Amti-sanility health-care food and preparation method thereof |
| CN110613113A (en) * | 2019-09-24 | 2019-12-27 | 武汉市农业科学院 | Preparation method of functional biological fermentation bee pollen |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1079367A (en) * | 1992-06-01 | 1993-12-15 | 四川大学 | The new method that edible pollen is made |
| CN1452888A (en) * | 2003-03-24 | 2003-11-05 | 浙江大学 | Fermenting and wall-breaking process for rape pollen |
| CN1488282A (en) * | 2003-08-15 | 2004-04-14 | 浙江大学 | Method for preparing artificial bee bread |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1079367A (en) * | 1992-06-01 | 1993-12-15 | 四川大学 | The new method that edible pollen is made |
| CN1452888A (en) * | 2003-03-24 | 2003-11-05 | 浙江大学 | Fermenting and wall-breaking process for rape pollen |
| CN1488282A (en) * | 2003-08-15 | 2004-04-14 | 浙江大学 | Method for preparing artificial bee bread |
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