CN86106482A - The method of pollen by use of eggshell and egg white broken wall - Google Patents
The method of pollen by use of eggshell and egg white broken wall Download PDFInfo
- Publication number
- CN86106482A CN86106482A CN86106482.8A CN86106482A CN86106482A CN 86106482 A CN86106482 A CN 86106482A CN 86106482 A CN86106482 A CN 86106482A CN 86106482 A CN86106482 A CN 86106482A
- Authority
- CN
- China
- Prior art keywords
- pollen
- egg white
- liquid
- eggshell
- broken wall
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Images
Landscapes
- Cosmetics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The method of the broken ancient piece of jade, round, flat and with a hole in its centre of a kind of pollen by use of eggshell and egg white is that eggshell egg white is added saline solution, extracting in water-bath, intermittently stir, transfer pH with hydrochloric acid, after filtration, collect filtrate, acetic acid is transferred pH, intensification makes albuminous degeneration, and through cooling, centrifugal, gained eggshell egg white causes sub-liquid to be mixed by a certain percentage with pollen again, the effect certain hour makes pollen break an ancient piece of jade, round, flat and with a hole in its centre.This method broken jade rate reaches 100%, and easy to operate, equipment is simple, and can follow the sterilization desensitization, and cost is low, the efficient height, and whole process is general only to need about two hours.
Description
Pollen is enjoyed the laudatory title of " king of food nutrition " with its nutrition comprehensively, is fabulous medicine source, can be used for cosmetics again.Therefore, the purposes of pollen more and more causes domestic and international expert's great attention.Nascent, middle life that pollen has and secondary three layers of hard wall, the quality of wall-breaking method can directly influence the utilization of pollen.At present, the domestic and international process for breaking wall of pollen that adopts is varied.The once disclosed breaking wall by fermentation method of known technology, Japan Patent and bee bread enzyme Jie broken wall method, the sporoderm-broken rate height, energy sterilization, but complicated operation, the time is long; The alternating temperature dry method broken wall of the once disclosed alternating temperature Wet-process wall breaking of DRP and Swiss Patent and China Liaoning, Jiangsu report, simple to operate, but sporoderm-broken rate only reaches about 90%, some nutrition destructible, and needing the low temperature facility, technological requirement is higher; Mixed solvent method is adopted in Guangxi, the sporoderm-broken rate height, and technology is simple, but unresolved sterilization desensitization.Linxiang, Hunan Province county bee product research institute discloses a kind of method of handling pollen with bee bread, pollen and 25～30 hours bee bread of brew are mixed thoroughly, added " southern wilsonii " below 30 ℃ honey catalytic liquid, after mixing thoroughly, in the tiling fermentation dish, cultivated about 35 ℃ 36～48 hours.Though this method is simple and easy to do, can not guarantee sporoderm-broken rate and sterilization desensitization well.
The object of the present invention is to provide a kind of broken wall fully, again can sterilization the process for breaking wall of pollen of simple and feasible of desensitization, the invention is characterized in from eggshell egg white, to extract and cause sub-liquid, carry out pollen broken wall and sterilization desensitization, pass through to add allicin liquid again, fresh-keeping and storing, the pollen broken wall overall process only needs about two hours, shell-broken effect is good, and sporoderm-broken rate reaches 100%.
The object of the present invention is achieved like this: will remove the eggshell egg white behind the yolk and give heat to 30 °～50 ℃ (0.08～0.19)
MNaCl-(0.01～0.07)
MHCl solution mixes in 1: 0.5～1: 4 ratio, smashs to pieces, with 1
NNaOH or 2
NHCl regulates the following water-bath extracting in PH4～7,30 °～50 ℃ 5～60 minutes, intermittently stirs.Filter with 10～20 order nylon cloths then, collect filtrate.Solids adds (0.08～0.19) more respectively according to the above ratio
MNaCl-(0.01～0.07)
MHCl solution repeats the extracting secondary, merges three times filtrate.HAC with 10～30% transfers filtrate PH3.5～6.5, puts and is warming up to 60 °～80 ℃ in the boiling water bath, and overall process continues 2～5 minutes, cooling immediately, again with 3000 rev/mins centrifugal 10～40 minutes, the gained supernatant is eggshell egg white and causes sub-liquid.During broken wall, earlier with pollen and (0.08～0.19)
MNaCl-(0.01～0.07)
MHCl solution wetting 1～3 hour in 1: 1～1: 3 ratio, causing sub-liquid in pollen and eggshell egg white again is that 1: 1～1: 5 ratio adds eggshell egg white immediately and causes sub-liquid, 20 °～40 ℃ allow it act on 1～4 hour, stir once every 5 minutes, can make the complete broken wall of pollen.Pollen behind the broken wall is centrifugal or filtration with 2500 rev/mins, can get broken pollen liquid.Press 5～15% of shell-broken liquid volume and add allicin liquid, can prevent to go mouldy storage life 4 months.Two days garlic of germination treatment is adopted in the preparation of allicin liquid, with (0.07～0.01)
MNaCl solution mixes in 1: 1 ratio, grinds homogenate, filter or 3000 rev/mins centrifugal 15 minutes, get supernatant and get final product.
The used chemical reagent of the present invention is commercially available analysis pure grade, and eggshell egg white is from commercially available fresh-laid egg or chilled storage egg.
Accompanying drawing one is the pollen grain electron micrograph (1500X) of not broken wall.
Accompanying drawing two is with the pollen grain electron micrograph (1200X) behind the broken wall of the present invention.
Further specify the present invention by the following examples.
Embodiment 1:
With 0.08
MNaCl～0.05
M125 milliliters of HCl solution give heat after 40 ℃ in thermostat water bath, take by weighing egg shell egg white 50 grams and mix with it, smash to pieces, with 2
NHCl transfers PH6, and 40 ℃ of following water-bath extractings 10 minutes are intermittently stirred, and filters collection filtrate then with 15 order nylon cloths.Solids adds 0.08 of 50 milliliters and 25 milliliters 40 ℃ more respectively
MNaCl～0.05
MHCl solution repeats the extracting secondary by this method, merges three times filtrate.Transfer filtrate PH to 46 with 20% HAC, put in the boiling water bath insulation to 75 ℃, 35 minutes duration, immediately with the flowing water cooling, with 3000 rev/mins centrifugal 20 minutes, collect supernatant, be eggshell egg white and cause sub-liquid.
Take by weighing pollen 5 grams, add 0.08
MNaCl～0.05
M10 milliliters of HCl solution, wetting 1 hour, add eggshell egg white immediately and cause 5 milliliters of sub-liquid, effect is 2 hours in 30 ℃ of insulating boxs, stirs once in per 5 minutes.Sampling is observed the broken wall situation at microscopically with 16 * 10,16 * 40 multiplication factors, and rate of breaking pollen wall reaches 100%.Through relevant department's check, the sterilization desensitization all reaches standard.Assay is up to specification.10% the ratio long-pending in pollen broken wall liquid adds allicin liquid, finds after 4 months to go mouldy.
Embodiment 2:
The concrete operations step is with embodiment 1.Experiment condition changes to some extent.0.12
MNaCl～0.6
MHCl solution gives heat to 35 ℃, and 100 milliliters add quail eggshell egg white 50 gram, and PH5.5 is transferred in 35 ℃ of water-bath extractings 20 minutes.Filtrate is transferred PH5 with 10%HAC, and boiling water bath is warming up to 60 ℃, continues 4.5 minutes, and centrifugation time is 15 minutes.Add allicin liquid rate of breaking pollen wall in 5% long-pending ratio of pollen broken wall liquid and reach 100%, the sterilization desensitization all reaches the standard of embodiment 1.
Embodiment 3:
The concrete operations step is with embodiment 1.Experiment condition changes to some extent.0.04
MNaCl～0.01
MHCl solution gives heat to 45 ℃, and 150 milliliters add snake eggshell egg white 50 gram, and PH6.5 is transferred in 45 ℃ of water-bath extractings 8 minutes, and filtrate is with 25% HAC accent PH5.5, and boiling water bath is warming up to 80 ℃, and the duration is 2.5 minutes, and centrifugation time is 30 minutes.15% the ratio long-pending in pollen broken wall liquid adds allicin, and rate of breaking pollen wall reaches 100%, and the sterilization desensitization all reaches the standard of embodiment 1.
It below is broken pollen liquid assay of the present invention.
1) check of sterilization conditions:
Total number of bacteria Escherichia coli bacterium colony staphylococcus, salmonella,
(individual/milliliter) (individual/liter) Shigella
1# 4,100 9 does not detect before handling
2＃ 4000 9 ″
Handle back 3# 2,700 9 "
4＃ 2300 4 ″
Former powder 5# 3,400 25 "
2) toxicity inspection: unit: ppm
The sample title
Shell-broken liquid shell-broken liquid pollen not
Interventions Requested
Lead does not detect
Arsenic＜0.2＜0.2 0.3
BHC 0.0035 0.0049 0.039
DDT 0.0015 0.0001 0.712
Organophosphor does not detect the positive
Claims (5)
1, a kind of method with the pollen by use of eggshell and egg white broken wall is characterized in that extracting from eggshell egg white and causes sub-liquid, carries out pollen broken wall and sterilization desensitization, again by adding allicin liquid fresh-keeping and storing.
2, method according to claim 1, it is characterized in that described extraction eggshell egg white cause sub-liquid with eggshell egg white with give heat 30 °～50 ℃ (0.08～0.19)
MNaCl-(0.01～0.07)
MHCl solution mixes in 1: 0.5～1: 4 ratio, transfers PH4～7,30 °～50 ℃ water-bath extracting 5～60 minutes, and extracting is three times repeatedly, and gained filtrate is transferred PH3.5～6.5 with 10～30% HAC, and boiling water bath is warming up to 60 °～80 ℃, lasting 2-5 minute.
3, method according to claim 1 is characterized in that described pollen broken wall and sterilization desensitization is with pollen and (0.08～0.19)
MNaCl-(0.01～0.07) MHCl solution is in 1: 1～1: 3 ratio wetting 1～3 hour, adds eggshell egg white immediately and causes sub-liquid, and the ratio that the eggshell egg white of adding causes sub-liquid and pollen is 1～1～5: 1,20 °～40 ℃ effects 1～4 hour.
4, method according to claim 1 is characterized in that described allicin liquid adds by the volume of pollen broken wall liquid 5～15%.
5,, it is characterized in that described allicin liquid is two days garlic of germination treatment and (0.07～0.1) according to claim 1 or 3 described methods
MNaCl solution mixes in 1: 1 ratio, grinds homogenate, filters or centrifugal gained supernatant.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN86106482A CN1012792B (en) | 1986-09-29 | 1986-09-29 | Wall-breaking method for pollen by use of eggshell and egg white |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN86106482A CN1012792B (en) | 1986-09-29 | 1986-09-29 | Wall-breaking method for pollen by use of eggshell and egg white |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN86106482A true CN86106482A (en) | 1988-04-06 |
| CN1012792B CN1012792B (en) | 1991-06-12 |
Family
ID=4803237
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN86106482A Expired CN1012792B (en) | 1986-09-29 | 1986-09-29 | Wall-breaking method for pollen by use of eggshell and egg white |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1012792B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1037061C (en) * | 1992-06-01 | 1998-01-21 | 四川大学 | Method for preparing edible pollen |
-
1986
- 1986-09-29 CN CN86106482A patent/CN1012792B/en not_active Expired
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1037061C (en) * | 1992-06-01 | 1998-01-21 | 四川大学 | Method for preparing edible pollen |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1012792B (en) | 1991-06-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Yan et al. | High intensity pulsed electric field as an innovative technique for extraction of bioactive compounds—A review | |
| CN104703611B (en) | For producing the multi-stage method of immunomodulator | |
| CA1108463A (en) | Process to obstruct the attack by viruses | |
| Ogur et al. | Desoxyribonucleic acid and the budding cycle in the yeasts | |
| CN113717297B (en) | Rose polysaccharide and preparation method and application thereof | |
| JP2743247B2 (en) | Lycopene oil production method | |
| CN1721546A (en) | Process for preparing II-type collagen compound | |
| CN102296014A (en) | Preparation method for selenium cordyceps wine | |
| CN86106482A (en) | The method of pollen by use of eggshell and egg white broken wall | |
| JP4307800B2 (en) | Immunostimulating composition containing mannooligosaccharide | |
| CN108840926A (en) | The high efficiency preparation method of liquid Poultry blood hemoglobin | |
| CN1130368C (en) | Process for preparing stachyose and its products and application | |
| KR101596219B1 (en) | Method for producing fermentation food | |
| CN1029540C (en) | Jellied fruit making technology using apple waste | |
| Szyczewski et al. | Preparation of Samples for Determining Heavy Metals Fe and Mn in Vegetable Oils: a Comparison of Different Extraction Methods. | |
| JP2007110989A (en) | Health supplementary food | |
| CN1023902C (en) | Method for preparation of humic acid, amino acid, ribose and nucleic acid mixture by biochemistry method | |
| Judith et al. | Evaluation of the proximate and elemental composition of traditional and industrial produced vinegar | |
| CN111772141A (en) | Honey pollen paste and preparation process thereof | |
| CN112876431B (en) | Method for extracting microalgae fucoxanthin, fucoxanthin and eicosapentaenoic acid | |
| JP2004222501A (en) | Healthy food using ant and earthworm and method for producing the same | |
| LU506424B1 (en) | Water-soluble illicium verum polysaccharide and preparation method and application thereof | |
| CN1073836A (en) | The halogen worm does (powder) production Technology | |
| CN1264423C (en) | Preparation method of selenium enriched garlic product possessing health care function and medical use | |
| CN1189301A (en) | Active pollen paste and its processing method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C13 | Decision | ||
| C14 | Grant of patent or utility model | ||
| C19 | Lapse of patent right due to non-payment of the annual fee |