CN103705711A - Chinese herbal compound for treating diabetes and preparation method thereof - Google Patents
Chinese herbal compound for treating diabetes and preparation method thereof Download PDFInfo
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- CN103705711A CN103705711A CN201410002188.3A CN201410002188A CN103705711A CN 103705711 A CN103705711 A CN 103705711A CN 201410002188 A CN201410002188 A CN 201410002188A CN 103705711 A CN103705711 A CN 103705711A
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Abstract
The invention discloses a Chinese herbal compound for treating diabetes and a preparation method thereof. The Chinese herbal compound is prepared from humifuse euphorbia herb, the root of red-rooted salvia, astragali radix, rhizoma anemarrhenae and coptis chinensis. In the Chinese herbal compound, the humifuse euphorbia herb has the main functions of clearing away heat and toxic materials, invigorating the circulation of blood and cooling blood and serves as monarch; the root of red-rooted salvia has the functions of promoting blood circulation to remove blood stasis and clearing away the heart fire to relieve restlessness; the coptis chinensis has the combined functions of clearing away heat and drying dampness as well as purging fire to remove toxin; the root of red-rooted salvia and the coptis chinensis serve as minister; the astragali radix has the functions of tonifying spleen and benefiting qi; the rhizoma anemarrhenae has the integrated functions of clearing heat and nourishing yin. The astragali radix and the rhizoma anemarrhenae serve as assistant and guide. The Chinese herbal compound has an obvious hypoglycemic effect.
Description
Technical field
The present invention relates to a kind of Chinese medicine compound for the treatment of diabetes and preparation method thereof.
Background technology
Diabetes spp is in motherland's medical science category of " quenching one's thirst ", ancient Chinese medicine doctor explicitly point out quench one's thirst for sick, no matter which kind of reason, no matter in what sick position, all by showing tremendous enthusiasm interior Sheng, looking angry pent-up is stagnant, consumption impairment of YIN Tianjin causes due to body fluid loss.Think that " pyretic toxicity " is the basic paathogenic factor of diabetes and the important pathogenesis.
Conventional hypoglycemic pcm is just like KELENING granule, YUQUAN WAN (Yuquan capsule), JIANGTANGSHU JIAONANG, SHENSHI JIANGTANG JIAONANG, blood sugar lowering first sheet etc. in the market, and these drug mains will, based on the scorching pathogenic pathogenesis of the deficiency of YIN, be take supplementing QI and nourishing YIN method for the treatment of as main.
Summary of the invention
The object of this invention is to provide a kind of Chinese medicine compound for the treatment of diabetes and preparation method thereof.
A kind of Chinese medicine composition for the treatment of diabetes provided by the invention, by Herba Euphorbiae Humifusae, Radix Salviae Miltiorrhizae, Radix Astragali, the Rhizoma Anemarrhenae and Rhizoma Coptidis form.
In above-mentioned Chinese medicine composition, described Herba Euphorbiae Humifusae, Radix Salviae Miltiorrhizae, Radix Astragali, the mass ratio of the Rhizoma Anemarrhenae and Rhizoma Coptidis is 1.32-5.34:1.76-7.12:0.88-6:0.88-3.56:1.
The method of preparing the Chinese medicine extract for the treatment of diabetes also belongs to a protection scope of the present invention, comprises the steps:
Described Chinese medicine composition is decocted in distilled water, obtain decoction liquor, decoction liquor is filtered and carried out concentrate drying again, obtain extract.
In said method, the mode of described decoction, for being first heated to boiling, continuing little fire and decocts after boiling.
In above-mentioned arbitrary described method, before described decoction, also comprise the step that described Chinese medicine composition is soaked in described distilled water.
In above-mentioned arbitrary described method, the time of described immersion is 2h;
The time that described little fire decocts is 1h.
In above-mentioned arbitrary described method, the volume ratio of described Chinese medicine composition and described distilled water is 1: 7.
The Chinese medicine extract that above-mentioned arbitrary described method obtains also belongs to protection scope of the present invention.
Traditional Chinese medicine liquid also belongs to a protection scope of the present invention, by above-mentioned Chinese medicine extract, is dissolved in distilled water and is made.
The application that above-mentioned Chinese medicine composition, above-mentioned Chinese medicine extract and/or above-mentioned traditional Chinese medicine liquid prevent and/or treat in the product of diabetes in preparation also belongs to protection scope of the present invention;
And/or,
The application in the product of preparation reduction blood sugar concentration of above-mentioned Chinese medicine composition, above-mentioned Chinese medicine extract and/or above-mentioned traditional Chinese medicine liquid also belongs to protection scope of the present invention.
In Chinese medicine compound provided by the invention, Herba Euphorbiae Humifusae heat-clearing and toxic substances removing, promoting blood circulation and cooling blood is monarch; Radix Salviae Miltiorrhizae blood circulation promoting and blood stasis dispelling, the relieving restlessness that clears away heart-fire, Rhizoma Coptidis heat clearing and damp drying, eliminating fire and detoxication is minister altogether; Radix Astragali invigorating the spleen and replenishing QI, Rhizoma Anemarrhenae clearing away heat and nourishing YIN is altogether for assistant makes.The present invention sets forth one's views so that the traditional Chinese medical science " pyretic toxicity " is theoretical, and treatment diabetes be take heat-clearing and toxic substances removing as main, are equipped with blood circulation promoting and blood stasis dispelling, and blood sugar lowering is evident in efficacy.
Accompanying drawing explanation
Fig. 1 is OGTT testing result before administration.
Fig. 2 is OGTT testing result after administration.
The specific embodiment
The experimental technique using in following embodiment if no special instructions, is conventional method.
In following embodiment, material used, reagent etc., if no special instructions, all can obtain from commercial channels.
Metformin hydrochloride (crude drug) is purchased from China Resources Double-Crane Pharmaceutical Co., Ltd., and lot number is A-11411212002.
Herba Euphorbiae Humifusae, Radix Salviae Miltiorrhizae, Radix Astragali, the Rhizoma Anemarrhenae and Rhizoma Coptidis are all purchased from Chinese medicine pharmacy of Peking University First Hospital.
Healthy male C 57 BL/6 J mouse, 25-30g, 9 week age, purchased from Beijing Vital River Experimental Animals Technology Co., Ltd..
Healthy male kk/Upj-Ay mice, 30-40g, 9 week age, purchased from China biotech inc, Fukang, Beijing.
Chinese medicine compound and the preparation thereof of embodiment 1, treatment diabetes
One, the preparation of Chinese medicine compound 1 extract: get Herba Euphorbiae Humifusae 158.4g, Radix Salviae Miltiorrhizae 211.2g, Radix Astragali 105.6g, Rhizoma Anemarrhenae 105.6g, it is 1.32:1.76:0.88:0.88:1 that Rhizoma Coptidis 120g amounts to ratio between each medicine of 700.8g(), to start decoction after 7 times of volume distilled water immersion 2h, be heated to boiling, after boiling, continue little fire and decoct 1h, filter, collect medicinal liquid, decoct altogether twice.The medicinal liquid that merges twice reduces pressure after cryoconcentration, through lyophilization, collects lyophilized powder 260.71g altogether after 2 days, and extraction ratio is 0.372.
Two, the preparation of Chinese medicine compound 2 extracts: get Herba Euphorbiae Humifusae 360g, Radix Salviae Miltiorrhizae 480g, Radix Astragali 240g, Rhizoma Anemarrhenae 240g, it is 3:4:2:2:1 that Rhizoma Coptidis 120g amounts to ratio between each medicine of 1440g() to start decoction after 7 times of volume distilled water immersion 2h, be heated to boiling, after boiling, continue little fire and decoct 1h, filter, collect medicinal liquid, decoct altogether twice.The medicinal liquid that merges twice reduces pressure after cryoconcentration, through lyophilization, collects lyophilized powder 551.93g altogether after 3 days, and extraction ratio is 0.383.
Three, the preparation of Chinese medicine compound 3 extracts: get Herba Euphorbiae Humifusae 640.8g, Radix Salviae Miltiorrhizae 854.4g, Radix Astragali 720g, Rhizoma Anemarrhenae 427.2g, it is 5.34:7.12:6:3.56:1 that Rhizoma Coptidis 120g amounts to ratio between each medicine of 2762.4g(), to start decoction after 7 times of volume distilled water immersion 2h, be heated to boiling, after boiling, continue little fire and decoct 1h, filter, collect medicinal liquid, decoct altogether twice.The medicinal liquid that merges twice reduces pressure after cryoconcentration, through lyophilization, collects lyophilized powder 1038.66g altogether after 5 days, and extraction ratio is 0.376.
The hypoglycemic activity of the Chinese medicine compound of embodiment 2, treatment diabetes
One, animal grouping
Be divided into following 6 groups:
Blank group: healthy male C 57 BL/6 J mouse, 25-30g, 9 week age, totally 8.
Diabetic groups: healthy male kk/Upj-Ay mice, 30-40g, 9 week age, totally 60.The male kk/Upj-Ay mice of health adaptability is raised 1 week, survey random blood sugar >11.1mmolL
-1or fasting glucose >7.0mmolL
-1be considered as diabetes model, select altogether 45, and it is divided into model control group, metformin hydrochloride group (200mg/kg), Chinese medicine compound 1 experimental group, Chinese medicine compound 2 experimental grouies, Chinese medicine compound 3 experimental grouies, 9 every group at random.
Two, respectively organize the dosage of mice
During blank group mouse stomach, give distilled water, according to 0.1ml10g
-1carry out gavage, every day, gavage was 1 time, and gavage is 2 weeks continuously.
During model control group gavage, give distilled water, according to 0.1ml10g
-1carry out gavage, every day, gavage was 1 time, and gavage is 2 weeks continuously.
The administration of metformin hydrochloride group is metformin hydrochloride, according to 200mg/kg, carries out gavage, during gavage, metformin hydrochloride is dissolved with distilled water, is mixed with 20mg/ml concentration, and every day, gavage was 1 time, and gavage is 2 weeks continuously.
Chinese medicine compound 1 experimental group administration is the extract of the Chinese medicine compound 1 of embodiment 1, according to 8g/kg, carries out gavage, during gavage, the extract of Chinese medicine compound 1 is dissolved with distilled water, is mixed with 0.8g/ml concentration, and every day, gavage was 1 time, and gavage is 2 weeks continuously.
Chinese medicine compound 3 experimental grouies are the extract of the Chinese medicine compound 3 of embodiment 1 preparation, according to 8g/kg, carry out gavage, during gavage, the extract of Chinese medicine compound 3 are dissolved with distilled water, are mixed with 0.8g/ml concentration, and every day, gavage was 1 time, and gavage is 2 weeks continuously.
Three, the detection of blood glucose before administration and after administration
(1) detection of index before administration
1, take the body weight of respectively organizing mice.
2, each group mice fasting be can't help to water 4h, get tail point venous blood and adopt glucose oxidase method to detect fasting glucose.
3, oral glucose tolerance (OGTT) detects
After having surveyed fasting glucose, by the dosage of 2g/kg, give immediately respectively to organize mouse stomach glucose solution, and after administration 30,60,120min collects Mouse Tail-tip venous blood, adopts glucose oxidase method to detect each time point blood glucose, calculates blood sugar concentration area under curve (AUCg).
Testing result is as shown in table 1.
Before table 1 administration, respectively organize Mouse Weight, fasting glucose and OGTT area under curve
As shown in Figure 1, in Fig. 1,1 group of ratio represents Chinese medicine compound 1 experimental group to OGTT testing result before administration, and 2 groups of ratios represent Chinese medicine compound 2 experimental grouies, and 3 groups of ratios represent Chinese medicine compound 3 experimental grouies.
(2) detection of index after administration
1, after gavage, take the body weight of respectively organizing mice the last time.
2, respectively organize mice the last time after gavage, water 4h is can't help in fasting, surveys fasting glucose, surveys 2h gastric infusion before fasting glucose.
3, oral glucose tolerance (OGTT) detects
After having surveyed fasting glucose, by the dosage of 2g/kg, give immediately respectively to organize mouse stomach glucose solution, and after administration 30,60,120min collects Mouse Tail-tip venous blood, adopts glucose oxidase method to detect each time point blood glucose,
Testing result is as shown in table 2.
Table 2 Chinese medicine compound is organized the impact of Mouse Weight, fasting glucose and OGTT area under curve to each
Note: 1. body weight: with model control group comparison, * * * P<0.001.
2. fasting glucose and OGTT area under curve (AUCg): with model control group comparison, * P<0.05, * * P<0.01, * * * P<0.001.
After administration, as shown in Figure 2, in Fig. 2,1 group of ratio represents Chinese medicine compound 1 experimental group to OGTT testing result, and 2 groups of ratios represent Chinese medicine compound 2 experimental grouies, and 3 groups of ratios represent Chinese medicine compound 3 experimental grouies.
Table 2 shows, after two weeks, Chinese medicine compound of the present invention all has the effect that reduces diabetic mice fasting glucose and reduce OGTT area under curve.
Claims (10)
1. treat a Chinese medicine composition for diabetes, by Herba Euphorbiae Humifusae, Radix Salviae Miltiorrhizae, Radix Astragali, the Rhizoma Anemarrhenae and Rhizoma Coptidis form.
2. Chinese medicine composition according to claim 1, is characterized in that: described Herba Euphorbiae Humifusae, Radix Salviae Miltiorrhizae, Radix Astragali, and the mass ratio of the Rhizoma Anemarrhenae and Rhizoma Coptidis is 1.32-5.34:1.76-7.12:0.88-6:0.88-3.56:1;
Described Herba Euphorbiae Humifusae, Radix Salviae Miltiorrhizae, Radix Astragali, the mass ratio of the Rhizoma Anemarrhenae and Rhizoma Coptidis is specially the ratio shown in (1)-(3) are arbitrary as follows:
（1）1.32:1.76:0.88:0.88:1；
（2）3:4:2:2:1；
（3）5.34:7.12:6:3.56:1。
3. a method of preparing the Chinese medicine extract for the treatment of diabetes, comprises the steps:
Chinese medicine composition described in claim 1 or 2 is decocted in distilled water, obtain decoction liquor, decoction liquor is filtered and carried out concentrate drying again, obtain extract.
4. method according to claim 3, is characterized in that: the mode of described decoction, for being first heated to boiling, continuing little fire and decocts after boiling.
5. according to the method described in claim 3 or 4, it is characterized in that: before described decoction, also comprise the step that described Chinese medicine composition is soaked in described distilled water.
6. according to the arbitrary described method of claim 3-5, it is characterized in that: the time of described immersion is 2h;
The time that described little fire decocts is 1h.
7. according to the arbitrary described method of claim 3-6, it is characterized in that: the volume ratio of described Chinese medicine composition and described distilled water is 1: 7.
8. the Chinese medicine extract being obtained by the arbitrary described method of claim 3-7.
9. a traditional Chinese medicine liquid, is dissolved in distilled water and is made by Chinese medicine extract claimed in claim 8.
10. the Chinese medicine composition described in claim 1 or 2, Chinese medicine extract claimed in claim 8 and/or traditional Chinese medicine liquid claimed in claim 9 prevent and/or treat the application in the product of diabetes in preparation;
And/or,
The application in the product of preparation reduction blood sugar concentration of Chinese medicine composition described in claim 1 or 2, Chinese medicine extract claimed in claim 8 and/or traditional Chinese medicine liquid claimed in claim 9.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105943819A (en) * | 2016-06-22 | 2016-09-21 | 北京大学第医院 | Traditional Chinese medicine alcohol extract for treating diabetic nephropathy and preparation method and application thereof |
| CN105943818A (en) * | 2016-06-22 | 2016-09-21 | 北京大学第医院 | Chinese traditional medicine water extract for treating diabetic nephropathy as well as preparation method and application thereof |
| CN108452115A (en) * | 2017-02-21 | 2018-08-28 | 成惠娜 | A kind of health instant powder that diabetes patient takes |
-
2014
- 2014-01-03 CN CN201410002188.3A patent/CN103705711B/en active Active
Non-Patent Citations (1)
| Title |
|---|
| 韩天雄等: "颜乾麟教授辨治糖尿病临证思维特点探析", 《浙江中医药大学学报》, vol. 36, no. 5, 31 May 2012 (2012-05-31) * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105943819A (en) * | 2016-06-22 | 2016-09-21 | 北京大学第医院 | Traditional Chinese medicine alcohol extract for treating diabetic nephropathy and preparation method and application thereof |
| CN105943818A (en) * | 2016-06-22 | 2016-09-21 | 北京大学第医院 | Chinese traditional medicine water extract for treating diabetic nephropathy as well as preparation method and application thereof |
| CN108452115A (en) * | 2017-02-21 | 2018-08-28 | 成惠娜 | A kind of health instant powder that diabetes patient takes |
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