CN105943819A - Traditional Chinese medicine alcohol extract for treating diabetic nephropathy and preparation method and application thereof - Google Patents
Traditional Chinese medicine alcohol extract for treating diabetic nephropathy and preparation method and application thereof Download PDFInfo
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- CN105943819A CN105943819A CN201610459903.5A CN201610459903A CN105943819A CN 105943819 A CN105943819 A CN 105943819A CN 201610459903 A CN201610459903 A CN 201610459903A CN 105943819 A CN105943819 A CN 105943819A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
- A61K36/8964—Anemarrhena
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/47—Euphorbiaceae (Spurge family), e.g. Ricinus (castorbean)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/481—Astragalus (milkvetch)
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/537—Salvia (sage)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
- A61K36/718—Coptis (goldthread)
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Abstract
The invention discloses a traditional Chinese medicine alcohol extract for treating diabetic nephropathy and a preparation method and application thereof. The preparation method comprises the following steps of: extracting a traditional Chinese medicine composition with an ethanol aqueous solution, filtering to obtain filtrate, and concentrating and drying to obtain the traditional Chinese medicine extract, wherein the traditional Chinese medicine composition comprises humifuse euphorbia herb, radix salviae miltiorrhizae, raw astragalus mongholicus, rhizoma anemarrhenae and coptis chinensis in a mass ratio of (1.32-5.34) to (1.76-7.12) to (0.88-6) to (0.88-3.56) to 1. The traditional Chinese medicine extract is obtained by carrying out alcohol extraction on the traditional Chinese medicine composition which comprises humifuse euphorbia herb, radix salviae miltiorrhizae, raw astragalus mongholicus, rhizoma anemarrhenae and coptis chinensis, and the method is simple. The traditional Chinese medicine extract is capable of lowering blood sugar concentration, lowering the content of urine micro albumin, lowering the content of urine urea, and lowering the ratio of the content of urine micro albumin to the content of the urine creatinine, and thus the traditional Chinese medicine extract can be used for preventing and/or treating diabetic nephropathy.
Description
Technical field
The present invention relates to a kind of Chinese medicine alcohol extraction thing treating diabetic nephropathy and preparation method and application, belong to Chinese medicine
Extract field.
Background technology
Diabetic nephropathy is a kind of serious Diabetic microvascular complication, is the main cause of induction end-stage renal failure,
Also the one of the main reasons be that diabetes are lethal, disabling.Treatment to diabetic nephropathy at present lacks effective specificity
Means.Chinese medicine have accumulated rich experience in treatment diabetic nephropathy field, is therefore expected to provide one to reduce
The Urinary Albumin Excretion of diabetic nephropathy patient, delays renal function of patients to go down, and the Chinese medicine improving life in patients carries
Take thing.
Summary of the invention
It is an object of the invention to provide a kind of Chinese medicine extract treating diabetic nephropathy and preparation method and application, this
The Chinese medicine extract that invention provides can reduce blood sugar concentration the content that can reduce microdose urine protein and reduce urine
The content of middle carbamide, therefore provides hope for diabetic nephropathy patients undergoing.
The preparation method of the Chinese medicine extract for the treatment of diabetic nephropathy provided by the present invention, comprises the steps:
Utilize ethanol water to extract Chinese medicine composition, filter to obtain filtrate, concentrated be drying to obtain described Chinese medicine extract；
Described Chinese medicine composition is made up of Herba Euphorbiae Humifusae, Radix Salviae Miltiorrhizae, Radix Astragali, the Rhizoma Anemarrhenae and Rhizoma Coptidis；
The mass ratio of described Herba Euphorbiae Humifusae, described Radix Salviae Miltiorrhizae, described Radix Astragali, the described Rhizoma Anemarrhenae and described Rhizoma Coptidis can be
1.32～5.34:1.76～7.12:0.88～6:0.88～3.56:1.
In above-mentioned preparation method, described Herba Euphorbiae Humifusae, described Radix Salviae Miltiorrhizae, described Radix Astragali, the described Rhizoma Anemarrhenae and described Rhizoma Coptidis
Mass ratio concretely 2.5:3.3:2.5:1.67:1.
In above-mentioned preparation method, the volumetric concentration of described ethanol water can be 40～80%, concretely 60%.
In above-mentioned preparation method, the consumption of described ethanol water can be: the 5～10 of described Chinese medicine composition weight
Times, 6 times of the most described Chinese medicine composition weight.
In above-mentioned preparation method, described in be extracted as percolation, decoct, reflux or any one extracting mode such as supersound extraction.
In above-mentioned preparation method, before described extraction, described Chinese medicine composition is placed in described ethanol water and carries out
Soak.
In above-mentioned preparation method, the time of described immersion can be 0.5～12h, concretely 30min.
In above-mentioned preparation method, the described mode extracting employing reflux, extract,；
The number of times of described reflux, extract, is 1～3 time, concretely 2 times；
The time of each described reflux, extract, can be 0.5～2h, concretely 1h.
Invention further provides the Chinese medicine extract prepared by said method.
Chinese medicine extract of the present invention can be used in prevention and/or treatment diabetic nephropathy.
The low dosage (2g/kg) of Chinese medicine extract of the present invention, middle dosage (4g/kg) and high dose (8g/kg)
All can reduce blood sugar concentration, reduce the content of microdose urine protein, the content reducing carbamide in urine and reduction urine
The ratio of the content of microdose urine protein and urine creatine in liquid.
Chinese medicine extract of the present invention can reduce the Urinary Albumin Excretion of diabetic nephropathy patient, delays renal function of patients to subtract
Move back.
Chinese medicine extract of the present invention, by Chinese medicine composition (Herba Euphorbiae Humifusae, Radix Salviae Miltiorrhizae, Radix Astragali, the Rhizoma Anemarrhenae and Rhizoma Coptidis group
Become) carry out alcohol extraction and obtain, method is simple；Chinese medicine extract of the present invention can reduce blood sugar concentration, reduce urine trace white
The content of microdose urine protein and urine creatine in the content of albumen, the content reducing carbamide in urine and reduction urine
Ratio, can be consequently used for prevention and/or the treatment of diabetic nephropathy.
Accompanying drawing explanation
Fig. 1 is that Chinese medicine alcohol extraction thing of the present invention is on the impact of microdose urine protein in KK-Ay urine.
Fig. 2 is that Chinese medicine alcohol extraction thing of the present invention is on the impact of carbamide in KK-Ay urine.
Fig. 3 is that Chinese medicine alcohol extraction thing of the present invention is on the impact of MA/UCREA in KK-Ay urine.
Fig. 4 is the Chinese medicine alcohol extraction thing of the present invention impact on KK-Ay creatinine in serum.
Fig. 5 is the Chinese medicine alcohol extraction thing of the present invention impact on KK-Ay urea in serum.
Fig. 6 is that Chinese medicine alcohol extraction thing of the present invention is on the impact of GA/GALB in KK-Ay serum.
Detailed description of the invention
Experimental technique used in following embodiment if no special instructions, is conventional method.
Material used in following embodiment, reagent etc., if no special instructions, the most commercially obtain.
Embodiment 1, the extraction of Chinese medicine extract
Weigh following prescription:
Herba Euphorbiae Humifusae 150g, Radix Salviae Miltiorrhizae 200g, Radix Astragali 150g, Rhizoma Anemarrhenae 100g, Rhizoma Coptidis 60g.
Add to the ethanol water that volume content is 60% of 6 times of quality after each Chinese medicine is mixed, soak 30min,
Reflux, extract, 2 times the most mutually, extracts 1 hour every time, and filtration, merging filtrate concentrate, be evaporated, be in the present invention
Medicament extract.
Chinese medicine extract prepared by the present embodiment is extractum shape, altogether 202g, and therefore 1g Chinese medicine extract is equivalent to 3.267g
Chinese medicine composition.
Embodiment 2, Chinese medicine extract of the present invention are on blood glucose and the impact of renal function
1, animal
Male spontaneous type 2 diabetes mellitus model KK-Ay mice 40,8w age, Beijing China Fukang biotechnology share
Company limited, licence numbering: SCXK (capital) 2014-0004；Healthy male C 57 BL/6 J mouse 10,8w
Age, Beijing Vital River Experimental Animals Technology Co., Ltd., licence numbering: SCXK (capital) 2012-0001.KK-Ay
Mice fed with high, Beijing HFK Bio-Technology Co., Ltd., licence numbering: SCXK (capital)
2014-0008；C57BL/6J mice normal diet is raised.All animals freely drink water, in room temperature (22 ± 1) DEG C,
In 12h periodicity of illumination SPF environment, adaptability is raised 1 week.Tail point takes blood and surveys KK-Ay mice random blood sugar
≥11.1mmol·L-1Or fasting glucose (fasting blood glucose, FBG) >=7.0mmol L-1I.e. it is considered as diabetes mould
Type.
2, each group mice dosage
Give distilled water during blank group mouse stomach, carry out gavage according to 0.1ml/10g, once a day, continuously
Gavage 12 weeks.
Give distilled water during model control group mouse stomach, carry out gavage according to 0.1ml/10g, once a day, continuously
Gavage 12 weeks.
Give distilled water during metformin hydrochloride group (positive controls) mouse stomach, carry out gavage according to 200mg/kg,
Being dissolved by metformin hydrochloride distilled water during gavage, be configured to 20mg/ml concentration, every day, gavage 1 time, connected
Continuous gavage 12 weeks.
Chinese medicine takes thing low dose group and carries out gavage according to 2g/kg, is dissolved by alcohol extraction thing distilled water, configuration during gavage
Become 0.8g/ml concentration, every day gavage 1 time, continuous 12 weeks.
Chinese medicine takes thing low dose group and carries out gavage according to 4g/kg, is dissolved by alcohol extraction thing distilled water, configuration during gavage
Become 0.8g/ml concentration, every day gavage 1 time, continuous 12 weeks.
Chinese medicine takes thing low dose group and carries out gavage according to 8g/kg, is dissolved by alcohol extraction thing distilled water, configuration during gavage
Become 0.8g/ml concentration, every day gavage 1 time, continuous 12 weeks.
3, impact on KK-Ay mouse blood sugar after being administered
3.1 fasting glucose (FBG)
All selected KK-Ay mices have become diabetes model the most.Compared with matched group, after being administered 8 weeks, metformin
And all administration groups all can be obviously improved mice fasting glucose (P ＜ 0.05)；After being administered 12 weeks, metformin, in
Medicament extract low dose group all can be obviously improved mice fasting glucose (P ＜ 0.05), and result is as shown in table 1.
The impact (χ ± s, n=8) on KK-Ay mice fasting glucose of the table 1 each process group
Note: compared with model group, * P ＜ 0.05, * * P ＜ 0.01, * * * P ＜ 0.001.
3.2 post-prandial glycemia (postprandial blood sugar, PPBS)
Compared with matched group, from being administered the 4th week, after being administered 4 weeks and 12 weeks, metformin and all administration groups are equal
Mice post-prandial glycemia (P ＜ 0.05) can be obviously improved；After being administered 8 weeks, metformin and Chinese medicine extract is low, neutralize
High dose group all can significantly improve mice post-prandial glycemia (P ＜ 0.05), and result is as shown in table 2.
The impact (χ ± s, n=8) on KK-Ay post-prandial glycemia of the table 2 each process group
Note: compared with model group, * P ＜ 0.05, * * P ＜ 0.01, * * * P ＜ 0.001.
4, impact on KK-Ay Mouse Kidney function after being administered
As shown in figs 1 to 6, by upper figure it can be seen that compared with matched group, after being administered 12 weeks, diformazan is double for result
Guanidine and each administration group all can reduce MA in urine (microdose urine protein), MA/UCREA (urine to a certain extent
Microalbumin/urine creatine), UREA (carbamide) value, wherein, metformin group and Chinese medicine extract is low, neutralize
High dose group reduces MA and has statistical significance (P ＜ 0.05)；Metformin group and Chinese medicine extract are low, medium and high
Dosage group reduces MA/UCREA and has statistical significance (P ＜ 0.05)；The low high dose group of Chinese medicine extract reduces
UREA has statistical significance (P ＜ 0.05).After being administered 12 weeks, metformin and each administration group are to CREA in serum
Impact be not statistically significant；Metformin group and each administration group all can reduce UREA in serum to a certain extent,
Wherein metformin group, Chinese medicine extract low, medium and high dosage group reduce UREA in serum and compare with model group and have
Statistical significance (P ＜ 0.05).
Claims (10)
1. treat a preparation method for the Chinese medicine extract of diabetic nephropathy, comprise the steps:
Utilize ethanol water to extract Chinese medicine composition, filter to obtain filtrate, concentrated be drying to obtain described Chinese medicine extract；
Described Chinese medicine composition is made up of Herba Euphorbiae Humifusae, Radix Salviae Miltiorrhizae, Radix Astragali, the Rhizoma Anemarrhenae and Rhizoma Coptidis；
The mass ratio of described Herba Euphorbiae Humifusae, described Radix Salviae Miltiorrhizae, described Radix Astragali, the described Rhizoma Anemarrhenae and described Rhizoma Coptidis is 1.32～5.34:
1.76～7.12:0.88～6:0.88～3.56:1.
Preparation method the most according to claim 1, it is characterised in that: the volumetric concentration of described ethanol water
It is 40～80%.
Preparation method the most according to claim 1 and 2, it is characterised in that: the consumption of described ethanol water
For: 5～10 times of described Chinese medicine composition weight.
4. according to the preparation method according to any one of claim 1-3, it is characterised in that be extracted as described in: percolation,
Decoct, reflux or supersound extraction.
5. according to the preparation method according to any one of claim 1-4, it is characterised in that: before described extraction, by institute
State Chinese medicine composition to be placed in described ethanol water and soak；
The time of described immersion is 0.5～12h.
6. according to the preparation method described in claim 4 or 5, it is characterised in that: described extraction uses reflux, extract,
Mode；
The number of times of described reflux, extract, is 2 times；
The time of each described reflux, extract, is 0.5～2h.
7. the Chinese medicine extract that prepared by method according to any one of claim 1-6.
8. the application in prevention and/or treatment diabetic nephropathy of the Chinese medicine extract described in claim 7.
9. Chinese medicine extract described in claim 7 is preparing following 1)-5) in application in any one product:
1) prevent and/or treat the product of diabetic nephropathy；
2) product of blood sugar concentration is reduced；
3) product of the content of microdose urine protein is reduced；
4) product of the content of carbamide in urine is reduced；
5) product of the ratio of the content of microdose urine protein and urine creatine in urine is reduced.
10. prevention and/or a method for treatment diabetic nephropathy, in using described in claim 7 to experimenter
The step of medicament extract；
The using dosage of described Chinese medicine extract is 2～8g/kg.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610459903.5A CN105943819A (en) | 2016-06-22 | 2016-06-22 | Traditional Chinese medicine alcohol extract for treating diabetic nephropathy and preparation method and application thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110772607A (en) * | 2019-10-21 | 2020-02-11 | 南方医科大学 | Traditional Chinese medicine composition for improving diabetic nephropathy and preparation thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103705711A (en) * | 2014-01-03 | 2014-04-09 | 北京大学第一医院 | Chinese herbal compound for treating diabetes and preparation method thereof |
-
2016
- 2016-06-22 CN CN201610459903.5A patent/CN105943819A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103705711A (en) * | 2014-01-03 | 2014-04-09 | 北京大学第一医院 | Chinese herbal compound for treating diabetes and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 洪金妮 等: "中药降糖复方对KK-Ay小鼠血糖的影响", 《2015年国际中西医结合内分泌代谢病学术研讨会暨第八次全国中西医结合内分泌代谢病学术年会讲义论文汇编》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110772607A (en) * | 2019-10-21 | 2020-02-11 | 南方医科大学 | Traditional Chinese medicine composition for improving diabetic nephropathy and preparation thereof |
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