CN103675280A - 一种检测丙肝核心抗原的标记物及试剂 - Google Patents
一种检测丙肝核心抗原的标记物及试剂 Download PDFInfo
- Publication number
- CN103675280A CN103675280A CN201310690679.7A CN201310690679A CN103675280A CN 103675280 A CN103675280 A CN 103675280A CN 201310690679 A CN201310690679 A CN 201310690679A CN 103675280 A CN103675280 A CN 103675280A
- Authority
- CN
- China
- Prior art keywords
- label
- hrp
- wash
- cag
- antibody
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 13
- 239000000427 antigen Substances 0.000 title abstract description 12
- 102000036639 antigens Human genes 0.000 title abstract description 12
- 108091007433 antigens Proteins 0.000 title abstract description 12
- 239000003550 marker Substances 0.000 title abstract description 6
- 108700039791 Hepatitis C virus nucleocapsid Proteins 0.000 title abstract 5
- 108010001336 Horseradish Peroxidase Proteins 0.000 claims abstract description 29
- 238000001514 detection method Methods 0.000 claims abstract description 26
- 238000006243 chemical reaction Methods 0.000 claims abstract description 9
- 239000007853 buffer solution Substances 0.000 claims abstract description 3
- 210000004185 liver Anatomy 0.000 claims description 13
- GBFLZEXEOZUWRN-VKHMYHEASA-N S-carboxymethyl-L-cysteine Chemical compound OC(=O)[C@@H](N)CSCC(O)=O GBFLZEXEOZUWRN-VKHMYHEASA-N 0.000 claims description 9
- 229960004399 carbocisteine Drugs 0.000 claims description 9
- UFULAYFCSOUIOV-UHFFFAOYSA-N cysteamine Chemical compound NCCS UFULAYFCSOUIOV-UHFFFAOYSA-N 0.000 claims description 6
- 229960003151 mercaptamine Drugs 0.000 claims description 6
- 230000009467 reduction Effects 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 4
- 238000004587 chromatography analysis Methods 0.000 claims description 3
- 238000009413 insulation Methods 0.000 claims description 3
- 239000002808 molecular sieve Substances 0.000 claims description 3
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 claims description 3
- NPAWNPCNZAPTKA-UHFFFAOYSA-M sodium;propane-1-sulfonate Chemical compound [Na+].CCCS([O-])(=O)=O NPAWNPCNZAPTKA-UHFFFAOYSA-M 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims 1
- 230000035945 sensitivity Effects 0.000 abstract description 16
- 239000000758 substrate Substances 0.000 abstract description 4
- 230000003197 catalytic effect Effects 0.000 abstract description 3
- 229920000642 polymer Polymers 0.000 abstract description 3
- 208000005176 Hepatitis C Diseases 0.000 abstract description 2
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 abstract 4
- JJVXHDTWVIPRBZ-VKHMYHEASA-N (2r)-2-[carboxy(methyl)amino]-3-sulfanylpropanoic acid Chemical compound OC(=O)N(C)[C@@H](CS)C(O)=O JJVXHDTWVIPRBZ-VKHMYHEASA-N 0.000 abstract 2
- 235000019766 L-Lysine Nutrition 0.000 abstract 2
- 239000004472 Lysine Substances 0.000 abstract 2
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 241000711549 Hepacivirus C Species 0.000 description 21
- 210000002966 serum Anatomy 0.000 description 16
- JQWHASGSAFIOCM-UHFFFAOYSA-M sodium periodate Chemical compound [Na+].[O-]I(=O)(=O)=O JQWHASGSAFIOCM-UHFFFAOYSA-M 0.000 description 8
- 238000000034 method Methods 0.000 description 7
- 101710132601 Capsid protein Proteins 0.000 description 4
- 238000002965 ELISA Methods 0.000 description 4
- 241000700605 Viruses Species 0.000 description 4
- 208000006454 hepatitis Diseases 0.000 description 4
- 231100000283 hepatitis Toxicity 0.000 description 4
- 208000015181 infectious disease Diseases 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 239000012505 Superdex™ Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 230000003053 immunization Effects 0.000 description 2
- 238000002649 immunization Methods 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000013207 serial dilution Methods 0.000 description 2
- 238000012795 verification Methods 0.000 description 2
- VLARLSIGSPVYHX-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) 6-(2,5-dioxopyrrol-1-yl)hexanoate Chemical compound O=C1CCC(=O)N1OC(=O)CCCCCN1C(=O)C=CC1=O VLARLSIGSPVYHX-UHFFFAOYSA-N 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 206010016654 Fibrosis Diseases 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 208000037581 Persistent Infection Diseases 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- -1 amino, sulfydryl Chemical group 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007882 cirrhosis Effects 0.000 description 1
- 208000019425 cirrhosis of liver Diseases 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000003636 conditioned culture medium Substances 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 231100000284 endotoxic Toxicity 0.000 description 1
- 230000002346 endotoxic effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 208000010710 hepatitis C virus infection Diseases 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 201000007270 liver cancer Diseases 0.000 description 1
- 208000014018 liver neoplasm Diseases 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002510 pyrogen Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000003375 selectivity assay Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
- G01N33/545—Synthetic resin
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/576—Immunoassay; Biospecific binding assay; Materials therefor for hepatitis
- G01N33/5767—Immunoassay; Biospecific binding assay; Materials therefor for hepatitis non-A, non-B hepatitis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/08—RNA viruses
- G01N2333/18—Togaviridae; Flaviviridae
- G01N2333/183—Flaviviridae, e.g. pestivirus, mucosal disease virus, bovine viral diarrhoea virus, classical swine fever virus (hog cholera virus) or border disease virus
- G01N2333/186—Hepatitis C; Hepatitis NANB
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2458/00—Labels used in chemical analysis of biological material
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Virology (AREA)
- Communicable Diseases (AREA)
- Tropical Medicine & Parasitology (AREA)
- Peptides Or Proteins (AREA)
Abstract
Description
Claims (5)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310690679.7A CN103675280B (zh) | 2013-12-17 | 2013-12-17 | 一种检测丙肝核心抗原的标记物及试剂 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310690679.7A CN103675280B (zh) | 2013-12-17 | 2013-12-17 | 一种检测丙肝核心抗原的标记物及试剂 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN103675280A true CN103675280A (zh) | 2014-03-26 |
CN103675280B CN103675280B (zh) | 2015-05-20 |
Family
ID=50313466
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201310690679.7A Active CN103675280B (zh) | 2013-12-17 | 2013-12-17 | 一种检测丙肝核心抗原的标记物及试剂 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN103675280B (zh) |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1020727A1 (en) * | 1998-07-30 | 2000-07-19 | Advanced Life Science Institute, Inc. | Method for assaying hepatitis c virus |
EP1083428A2 (en) * | 1999-08-19 | 2001-03-14 | Kyowa Medex Co., Ltd. | Method and reagent for the detection or determination of HCV core antigens |
CN101419238A (zh) * | 2008-12-04 | 2009-04-29 | 山东莱博生物科技有限公司 | 丙型肝炎病毒核心抗原化学发光酶联免疫检测试剂盒 |
CN102775334A (zh) * | 2012-06-12 | 2012-11-14 | 宁波市镇海海德生化科技有限公司 | L-赖氨酸-s-羧甲基-l-半胱氨酸盐的生产工艺 |
CN102890154A (zh) * | 2012-10-12 | 2013-01-23 | 武汉康苑生物医药科技有限公司 | 丙型肝炎病毒核心抗原时间分辨免疫荧光分析法及检测试剂盒 |
-
2013
- 2013-12-17 CN CN201310690679.7A patent/CN103675280B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1020727A1 (en) * | 1998-07-30 | 2000-07-19 | Advanced Life Science Institute, Inc. | Method for assaying hepatitis c virus |
EP1083428A2 (en) * | 1999-08-19 | 2001-03-14 | Kyowa Medex Co., Ltd. | Method and reagent for the detection or determination of HCV core antigens |
CN101419238A (zh) * | 2008-12-04 | 2009-04-29 | 山东莱博生物科技有限公司 | 丙型肝炎病毒核心抗原化学发光酶联免疫检测试剂盒 |
CN102775334A (zh) * | 2012-06-12 | 2012-11-14 | 宁波市镇海海德生化科技有限公司 | L-赖氨酸-s-羧甲基-l-半胱氨酸盐的生产工艺 |
CN102890154A (zh) * | 2012-10-12 | 2013-01-23 | 武汉康苑生物医药科技有限公司 | 丙型肝炎病毒核心抗原时间分辨免疫荧光分析法及检测试剂盒 |
Non-Patent Citations (2)
Title |
---|
GIANCARLO ICARDI 等: "Novel Approach To Reduce the Hepatitis C Virus (HCV) Window Period: Clinical Evaluation of a New Enzyme-Linked Immunosorbent Assay for HCV Core Antigen", 《JOURNAL OF CLINICAL MICROBIOLOGY》 * |
KATSUMI AOYAGI 等: "Development of a Simple and Highly Sensitive Enzyme Immunoassay for Hepatitis C Virus Core Antigen", 《JOURNAL OF CLINICAL MICROBIOLOGY》 * |
Also Published As
Publication number | Publication date |
---|---|
CN103675280B (zh) | 2015-05-20 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN103314297B (zh) | 免疫层析测试条及其制造方法 | |
CN104360060A (zh) | 一种基于微流控芯片的肺炎支原体和流感病毒特异抗体IgM的检测方法 | |
CN105122060A (zh) | 样本中的血红蛋白A1c的免疫测定方法 | |
WO2022107737A1 (ja) | SARS-CoV-2の検出キットおよびSARS-CoV-2の検出方法 | |
EP3339862A1 (en) | Immunological detection method and test strip used therefor | |
JP4704662B2 (ja) | 免疫学的ラテックス比濁分析方法及びその試薬 | |
WO2021232714A1 (zh) | 一种新型冠状病毒IgM抗体的酶联免疫法检测试剂盒 | |
Lakos | Interference in antiphospholipid antibody assays | |
WO2015037635A1 (ja) | インフルエンザウイルスの免疫測定における検体処理方法及び免疫測定法 | |
CN110818800A (zh) | 一种通过构建桥连复合物间接检测目的分析物的检测方法 | |
JP6304231B2 (ja) | 未感作ラテックス試薬の劣化防止方法 | |
WO2013146977A1 (ja) | 免疫分析方法及び試薬 | |
JP6729995B2 (ja) | 免疫分析方法及び試薬 | |
JP2009162535A (ja) | 固相作製用試薬及び該試薬を使用して作製した固相 | |
CN109633163B (zh) | 降钙素原/c反应蛋白二合一检测试剂盒 | |
CN103675280B (zh) | 一种检测丙肝核心抗原的标记物及试剂 | |
EP4365195A1 (en) | Antibody against nucleocapsid protein of sars-cov-2 | |
CN102253225B (zh) | 一种东南亚型α地中海贫血检测试剂盒 | |
CN114689869A (zh) | 试纸条、制备方法及pla2r、thsd7a、nell-1自身抗体联合定量检测方法 | |
CN109187943B (zh) | 一种抗干扰试剂杯以及试剂杯内抗干扰涂层的制备方法 | |
CN102433339B (zh) | 一种HCV核酸适配体及其在制备HCV-cAg检测试剂盒中的应用 | |
KR20230043100A (ko) | 시그널의 저하를 개선한 검사 시약 | |
WO2017090103A1 (ja) | 免疫分析方法及び試薬キット | |
CN101975857B (zh) | 病毒解裂剂及解裂病毒抗原抗体复合物并检测hcv抗原的方法 | |
CN105785046A (zh) | 一种高性能的人血液免疫球蛋白a检测试剂盒 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A marker and reagent for detecting hepatitis C core antigen Effective date of registration: 20211101 Granted publication date: 20150520 Pledgee: Overpass Branch of Jinan Rural Commercial Bank Co.,Ltd. Pledgor: BIOBASE BIODUSTRY (SHANDONG) Co.,Ltd. Registration number: Y2021370000122 |
|
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20220624 Granted publication date: 20150520 Pledgee: Overpass Branch of Jinan Rural Commercial Bank Co.,Ltd. Pledgor: BIOBASE BIODUSTRY (SHANDONG) Co.,Ltd. Registration number: Y2021370000122 |
|
PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A marker and reagent for detecting hepatitis C core antigen Effective date of registration: 20230206 Granted publication date: 20150520 Pledgee: Overpass Branch of Jinan Rural Commercial Bank Co.,Ltd. Pledgor: BIOBASE BIODUSTRY (SHANDONG) Co.,Ltd. Registration number: Y2023980032056 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20230531 Granted publication date: 20150520 Pledgee: Overpass Branch of Jinan Rural Commercial Bank Co.,Ltd. Pledgor: BIOBASE BIODUSTRY (SHANDONG) Co.,Ltd. Registration number: Y2023980032056 |
|
PC01 | Cancellation of the registration of the contract for pledge of patent right |