CN103669006A

CN103669006A - Method for performing biological pretreatment on cotton woven fabric by use of helicase

Info

Publication number: CN103669006A
Application number: CN201210342754.6A
Authority: CN
Inventors: 袁久刚; 王强; 范雪荣; 王平; 周斌
Original assignee: Jiangnan University
Current assignee: Jiangnan University
Priority date: 2012-09-14
Filing date: 2012-09-14
Publication date: 2014-03-26
Anticipated expiration: 2032-09-14
Also published as: CN103669006B

Abstract

蜗牛酶是从蜗牛嗉囊或消化道中提取的复合酶，主要含有纤维素酶、果胶酶、淀粉酶、蛋白酶等20多种酶，是蜗牛综合利用开发的副产物。但是在实际加工过程中，蜗牛的内脏往往被抛弃掉，这造成了酶资源的很大浪费。对于纺织品前处理而言，蜗牛酶所含的果胶酶、淀粉酶和纤维素酶是一种很好的复合酶体系。本发明将蜗牛酶这一天然的复合酶体系应用到棉机织物的生物精练工艺中，利用蜗牛酶的水解作用去除棉纤维中的果胶、蜡质和其他杂质，从而达到提高棉织物润湿性和白度的目的。采用本发明对棉织物进行精练，不仅具有环保和节能的特点，而且可以综合利用蜗牛加工的副产物，符合当前可持续发展的要求，有利于染整行业以及蜗牛农产品加工的综合发展。Snail enzyme is a compound enzyme extracted from snail crop or digestive tract, mainly containing more than 20 kinds of enzymes such as cellulase, pectinase, amylase, protease, etc., and is a by-product of comprehensive utilization and development of snails. However, in the actual processing process, the internal organs of snails are often discarded, which causes a great waste of enzyme resources. For textile pretreatment, the pectinase, amylase and cellulase contained in helicase is a good complex enzyme system. The invention applies the natural compound enzyme system of helicase to the bioscouring process of cotton woven fabrics, and uses the hydrolysis of helicase to remove pectin, wax and other impurities in cotton fibers, so as to improve the wetting of cotton fabrics. The purpose of sex and whiteness. Using the invention to scouring cotton fabric not only has the characteristics of environmental protection and energy saving, but also can comprehensively utilize the by-products of snail processing, meets the current sustainable development requirements, and is beneficial to the comprehensive development of dyeing and finishing industry and snail agricultural product processing.

Description

A kind of glusulase that utilizes carries out the method for Bio-Pretreatment to cotton fabrics

Technical field

Utilize glusulase cotton fabrics to be carried out to a method for Bio-Pretreatment, belong to COTTON FABRIC dyeing and finishing processing pretreatment technology field.

Background technology

It is food that occurring in nature snail mainly be take blade and the tender shoots of plant, therefore, in long-term evolutionary process, has produced the biology enzyme that can digest various plant components in its body.1898, Bi Deerman found to exist a large amount of enzymes such as cellulase, hemicellulase, seminase and proteolytic enzyme in the glandula digestive of snail.Nineteen twenty-two, victory adds first separated from the glandula digestive of snail and has extracted this mixed enzyme, and is referred to as " glusulase ".China scientific worker also in 1974 respectively from Achatina and the brown glandula digestive with collar extension spiral shell, successfully separation and extraction glusulase.At present, glusulase has tentatively been realized suitability for industrialized production at home.

According to research reports, average every milliliter of snail digestive juice can extract 10-130mg dry state enzyme preparation.Primary Study shows mainly to contain in glusulase 20 plurality of enzymes such as cellulase, pectase, amylase and protease.What wherein content was maximum is respectively cellulase and pectase, and these two kinds of enzymes are the most frequently used enzyme preparations of Bio-Enzyme Pretreatment of Cotton Fabric.Wherein, cellulase can be removed impurity on fiber and the fine hair of fabric face by hydrocellulose, improves hygroscopicity and the fineness of fabric; Pectase can decompose pectin contained in cotton fiber, improves the wettability of fabric.Other contained enzymes of glusulase in addition, as: hemicellulase, seminase and protease etc. can with cellulase and pectase synergy, the impurity on cotton fiber also will be played to certain removal effect as hemicellulose and protein etc.Therefore,, by being rich in the biology enzyme pre-treatment of the glusulase of plurality of enzymes component for COTTON FABRIC, can produce good scouring result.

In addition,, in recent years along with the development of biological enzyme technology, the biology enzyme of COTTON FABRIC is concise, and also from single enzyme, concise to develop into complex enzyme concise.This is mainly because enzyme has selectivity, and single enzyme can only be removed certain impurity on fabric, so the concise improvement to fabric property of traditional single enzyme is limited, fabric property promotes comprehensive not.Therefore, adopt complex enzyme system to carry out the concise concise inevitable development trend of following biology enzyme that become to fabric.Glusulase is a kind of so concise natural complex enzyme system of COTTON FABRIC that meets just.So it is concise to adopt this enzyme to carry out COTTON FABRIC, not only there is the environmental protection of biological treatment, the feature such as energy-conservation, and can fully utilize the accessory substance of snail processing, be conducive to the comprehensive development of dyeing and finishing industry and snail processing of farm products.

Summary of the invention

The present invention is incorporated into this natural compound concise enzyme system of glusulase in cotton fabrics pretreatment process, utilize the multiple biology enzyme components such as the contained pectase of glusulase, cellulase, amylase and protease to carry out Bio-Pretreatment to cotton fabrics grey cloth, remove the contained impurity such as pectin, wax, starch size, lignin and pigment on cotton fiber, finally reach the object that improves COTTON FABRIC wettability and whiteness.Adopt the present invention not only can solve the poor problem of single enzyme scouring result, and can improve the comprehensive utilization ratio of snail processing byproduct, promote the sustainable development of heliculture industry.

Main solution of the present invention:

A kind of technique of utilizing glusulase to carry out Bio-Pretreatment to cotton fabrics mainly adopts semi-continuous rolling and piling biology enzyme method for refining.Its technological process mainly consists of the following components: first destarch cotton fabrics carries out hot water or low alkali preliminary treatment, pad afterwards the concise liquid of glusulase and be incubated and bank up, finally by overheated washing (having the enzyme effect of going out concurrently), room temperature washing, dry, finally complete the bioanalysis pre-treatment of cotton fabrics.

1. cotton fabrics preliminary treatment

Hot-water pretreatment method: the cotton fabrics after destarch is first carried out to preliminary treatment in the hot water of 90-100 ℃, time is 2-10min, and bath raio 1: 50 (fabric weight: water volume), after being disposed, room temperature washing 3 times, dries (pick-up 100%) afterwards.

Low alkali preprocess method: the cotton fabrics after destarch is first padded to (alkali lye formula: NaOH 10g/L, non-ionic surface active agent 1g/L in the alkali lye of 90 ℃, two soak two rolls, pick-up 100%), time 2-5s; bath raio 1: 50; 120 ℃ of high temperature steaming 1-5min afterwards, last room temperature washing 3 times, dries (pick-up 100%).

2. glusulase is concise

(1) configuration of enzyme liquid: glusulase solid (Achatina product glusulase) is first melted with 40-50 ℃ of warm water, join afterwards in the buffer solution of being prepared by sodium hydrogen phosphate and citric acid, the nonionic or the anion surfactant that add 0-2g/L, stir and filter again.The concise liquid concentration of glusulase of final configuration is 2-10g/L, pH value 4.5-6.5.

(2) enzyme is processed: pretreated fabric is padded to glusulase liquid with the speed of a motor vehicle of 10-20m/min, and the mode of padding is two to soak two and roll, and the temperature of padding enzyme treatment fluid is room temperature to 65 ℃, and pick-up is 80%-100%, rolls laggard windrow and puts.

3. insulation is banked up

Temperature has material impact to the scouring result of enzyme, and cotton fabrics need be banked up after padding enzyme liquid under 50-65 ℃ of unsaturated steam condition, and the time, temperature was high between 30-90min, and the time of banking up is short, and the low time of banking up of temperature is long.

4. washing and oven dry

The fabric of heap postpone need be first with the hot wash of 95 ℃ of left and right 1 time, about 5-20s of time, this process not only can be removed most of impurity of enzyme processing but also glusulase is had to kill activity, prevent the continuation hydrolysis of enzyme to COTTON FABRIC, after hot wash, fabric with room temperature washing 3 times, is finally dried again.

The specific embodiment:

The present invention illustrates by following examples, but the present invention is not limited to following embodiment, and before and after not departing from, in the scope of described aim, change is included in technical scope of the present invention.

Embodiment 1

Sample: the textile poplin cloth fabric of destarch

A. preliminary treatment: the textile poplin cloth after destarch is first processed to 10min in the hot water of 95 ℃, bath raio 1: 50, after being disposed, room temperature washing 3 times, dries (pick-up 100%) afterwards.

B. pretreated fabric is padded to glusulase liquid (enzyme concentration 5g/L, pH=6, temperature 50 C, paregal O concentration 2g/L) with the speed of 10m/min, the mode of padding is two to soak two and roll, and pick-up is 100%, rolls laggard windrow and puts.

C. textile poplin cloth is banked up under 65 ℃ of unsaturated steam conditions, time 45min.

D., after banking up, first use the hot wash 1 time of 95 ℃, about 10s of time, room temperature washing afterwards 3 times, finally dries.

After said method is processed, the wetting time of textile poplin cloth is promoted to 8s by the 24s before processing, and whiteness value Wr is promoted to 78.5. by 56.8

Embodiment 2

Sample: destarch Quality Pure Cotton Yarn Production card fabric

A. preliminary treatment: by Quality Pure Cotton Yarn Production card elder generation (the alkali lye formula: NaOH 10g/L, non-ionic surface active agent 1g/L in the thermokalite of 90 ℃ after destarch, two soak two rolls, pick-up 100%,) pad, time 2s, bath raio 1: 50, afterwards 120 ℃ of high temperature steaming 4min, last room temperature washing 3 times, dries (pick-up 100%).

B. pretreated fabric is padded to glusulase liquid (enzyme concentration 5g/L, pH=6.5, temperature 50 C, paregal O concentration 1g/L) with the speed of 15m/min, the mode of padding is two to soak two and roll, and pick-up is 100%, rolls laggard windrow and puts.

C. textile poplin cloth is banked up under 60 ℃ of unsaturated steam conditions, time 30min.

After said method is processed, the wetting time of Quality Pure Cotton Yarn Production card is promoted to 3s by the 20s before processing, and whiteness value Wr is promoted to 82.3. by 58.5

Embodiment 3

Sample: destarch Weaving Pure Cotton Khaki Drills fabric

A. preliminary treatment: by Weaving Pure Cotton Khaki Drills elder generation (the alkali lye formula: NaOH 10g/L, non-ionic surface active agent 1g/L in the thermokalite of 90 ℃ after destarch, two soak two rolls, pick-up 100%,) pad, time 2s, bath raio 1: 50, afterwards 120 ℃ of high temperature steaming 3min, last room temperature washing 3 times, dries (pick-up 100%).

B. pretreated fabric is padded to glusulase liquid (enzyme concentration 10g/L, pH=4.5, temperature 50 C, neopelex concentration 1g/L) with the speed of 10m/min, the mode of padding is two to soak two and roll, and pick-up is 100%, rolls laggard windrow and puts.

C. Weaving Pure Cotton Khaki Drills is banked up under 50 ℃ of unsaturated steam conditions, time 60min.

After said method is processed, the wetting time of Weaving Pure Cotton Khaki Drills is promoted to 3.5s by the 15s before processing, and whiteness value Wr is promoted to 80.3 by 60.5.

Claims

1. A method utilizing helicase to carry out biological pretreatment to cotton woven fabrics, its technological process comprises the following aspects:

(1) Pretreatment: Cotton woven fabrics are first pretreated with hot water or low-concentration caustic soda;

(2) Enzyme treatment: padding the pretreated woven fabric with snail enzyme solution, the padding method is two dipping and two padding, the temperature of the padding treatment solution is from room temperature to 65°C, and the excess rate is 80%-100%;

(3) Insulation stacking: After padding the enzyme solution, stack it under the condition of unsaturated steam at 50-65°C for 30-90min;

(4) Washing and drying: After the stacking is completed, the fabric needs to be washed once with hot water at about 95°C for about 5-20s. After washing in hot water, the fabric is washed 3 times in room temperature water, and finally dried.

2. according to a kind of method that utilizes helicase to carry out biological pretreatment to cotton woven fabric according to right 1, it is characterized in that the biological enzyme that adopts is novel natural compound enzyme system---helicase, and the source of this enzyme can be France Among snails such as snails, Chinese snails, garden snails, and agate snails, the enzyme performance of the brown cloud agate snail is better.

3. a kind of method utilizing helicase to carry out biological pretreatment to cotton woven fabric according to right 1, it is characterized in that the desizing cotton woven fabric that this technique is applicable comprises cotton woven fabrics such as plain cloth, gauze card, poplin, khaki fabric.

4. A method of utilizing helicase to carry out biological pretreatment of cotton woven fabrics according to right 1, characterized in that the helicase liquid used in the method consists of: helicase concentration 2-10g/L, non-ionic or anionic The surfactant concentration is 0-2g/L, the buffer solution is disodium hydrogen phosphate and citric acid system, and the pH value is between 4.5-6.5.