CN105401448B - A kind of bafta enzymatic scouring method that crude enzyme liquid is produced based on bolt bacterium - Google Patents
A kind of bafta enzymatic scouring method that crude enzyme liquid is produced based on bolt bacterium Download PDFInfo
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- CN105401448B CN105401448B CN201510954133.7A CN201510954133A CN105401448B CN 105401448 B CN105401448 B CN 105401448B CN 201510954133 A CN201510954133 A CN 201510954133A CN 105401448 B CN105401448 B CN 105401448B
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- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M16/00—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
- D06M16/003—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic with enzymes or microorganisms
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- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06L—DRY-CLEANING, WASHING OR BLEACHING FIBRES, FILAMENTS, THREADS, YARNS, FABRICS, FEATHERS OR MADE-UP FIBROUS GOODS; BLEACHING LEATHER OR FURS
- D06L1/00—Dry-cleaning or washing fibres, filaments, threads, yarns, fabrics, feathers or made-up fibrous goods
- D06L1/12—Dry-cleaning or washing fibres, filaments, threads, yarns, fabrics, feathers or made-up fibrous goods using aqueous solvents
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M2101/00—Chemical constitution of the fibres, threads, yarns, fabrics or fibrous goods made from such materials, to be treated
- D06M2101/02—Natural fibres, other than mineral fibres
- D06M2101/04—Vegetal fibres
- D06M2101/06—Vegetal fibres cellulosic
Abstract
The invention discloses a kind of bafta enzymatic scouring method that crude enzyme liquid is produced based on bolt bacterium, belong to textile technology field.The bolt bacterium Trametes sp.LEF01 that the inventive method is screened using nature and the crude enzyme liquid progress concise processing of bafta through the production of cotton seed hulls based biomass induction fermentation, crude enzyme liquid contain can Synergistic degradation cotton seed hulls a variety of enzyme components such as laccase, cellulase, hemicellulase, pectase, each enzyme has extraordinary compatibility in system, overcome single enzyme and compound brought poor compatibility problem, and while preferable wetability is obtained, the degraded for realizing cotton seed hulls is removed, and fundamentally solves the technology barrier of bafta enzymatic scouring industrialized production.
Description
Technical field
The present invention relates to a kind of bafta enzymatic scouring method that crude enzyme liquid is produced based on bolt bacterium, belong to textile technology field.
Background technology
The pre-treatment of bafta, including former cloth prepares, singed, desizing, concise (kiering), bleaching, open-width, squezzing, drying
And mercerizing process, to remove the impurity such as the pectin in fiber, wax, cotton seed hulls and slurry, improve the outward appearance and inherent matter of fabric
Amount.
When cotton fiber grows, there is natural impurity (pectic substance, waxy substance, nitrogen substance etc.) association together.Bafta is passed through
Cross after desizing, most of slurry and part natural impurity have been removed, but most of natural impurity in cotton fiber, such as wax
Matter, pectin substance, nitrogen substance, cotton seed hulls and part finish and a small amount of slurry etc. are also remained on bafta, make bafta cloth
Face is more yellow, poor permeability, it is impossible to adapt to dyeing, the requirement of stamp processing.Simultaneously as there is the presence of cotton seed hulls, largely effect on
The presentation quality of cotton.In order that bafta has certain water imbibition, be conducive to the absorption of dyestuff in dyeing process, expand
Dissipate, will also be by concise, to remove most of residual impurity in cotton fiber after desizing.
For a long time, bafta is concise uses alkali pretreatment always, using caustic soda and other boiling-off additives and pectic substance,
Chemical degradation reaction or emulsification, Swelling Functions etc. occur for waxy substance, nitrogen substance, cotton seed hulls, and impurity is made after washing
Stripped from fabric.The process consumes substantial amounts of water and alkali, pH value of waste water, the COD value severe overweight of discharge, have impact on ecological ring
Border, and stronger alkali and high-temperature process can also damage fiber.Enhancing with people to resource and environmental protection consciousness, urgently
Need to rebuild traditional industry using biotechnology.Foreign countries have started biology enzyme in textile since the 1980s
The upsurge of upper application study, but emphasis is biology enzyme Final finishing, and to application of the biology enzyme in terms of cotton pretreatment until
Last century does not just begin one's study.Novozymes Company of the nineties Denmark and Osaka, Japan university develop respectively alkaline pectase and
After protopectinase, the application of biology enzyme pretreatment technology industrially is gradually developed.
Both at home and abroad about the research of Bio-Enzyme Pretreatment of Cotton Fabric, initially pay attention to the selection of enzyme, single enzyme is such as emphatically
The effect to raising cotton fiber wetability such as pectase, lipase.Under the same terms, compared to pectase, cellulase makes
With then there may be weightless and strength loss.Start to turn to the complex enzyme constituted after the different enzyme mixing of research in recent years to improving
The synergy of cotton fiber wetability, its emphasis is pectase and cellulase, or adds protease, lipase, and it is right after using to spell
Cotton pretreatment has certain synergy, can effectively remove the impurity on cotton fiber, but different enzyme zymologic properties are not
Equally, often there are problems that poor compatibility, and also exist in terms of wetability improves with cotton seed hulls removal compared with alkali process
A certain distance.Moreover, the beginning of this century, the country is proposed a variety of business complex enzyme formulations, but these products are mostly biology enzyme
With the product of chemicals (the especially mixture of alkali) phase blending, and up to 10g/L~15g/L (100% must be used when using
H2O2) hydrogen peroxide carry out oxygen bleaching processing, its alkali consumption is high, and oxidation is fierce, easily makes fabric damage.In addition, these " complex enzymes " are to the greatest extent
Pipe various types, but because the factors such as effect and environmental protection are not yet widely applied in production.
In a word, existing bafta enzymatic scouring technology either uses single enzyme or complex enzyme, is removed to cotton seed hulls
Undesirable in effect, particularly the fabric higher to cloth cover cotton seed hulls is (even if wetability obtains good effect) even more so, very
Many documents or patent report avoid this point (not carrying cotton seed hulls treatment effect).However, cotton seed hulls naked eyes are visible, it is that factory is actual
Most intuitively one of cotton scouring result in production.Though this, which is also enzymatic scouring, there are a large amount of reports, industrialization life is still not implemented at present
The main reason of production.
The content of the invention
In order to overcome current biology enzyme refining process there are problems that cotton seed hulls be difficult to remove, particularly give birth to
The problem of thing enzyme process is for the relatively poor bafta of some qualities (cloth cover contains more cotton seed hulls) poor processing effect, the present invention
The bolt bacterium screened using nature is simultaneously produced through cotton seed hulls constituent analogue (sodium lignin sulfonate) induction fermentation
Crude enzyme liquid (containing can Synergistic degradation cotton seed hulls a variety of enzyme components such as laccase, cellulase, hemicellulase, pectase) carry out
The concise processing of bafta, employing each enzyme in a bacterium multienzyme zymotechnique of low cost, system has extraordinary compatibility, gram
Take single enzyme and compounded brought poor compatibility problem, and while preferable wetability is obtained, realize cotton seed hulls
Degraded is removed, and fundamentally solves the technology barrier of bafta enzymatic scouring industrialized production.
Present invention screening first obtains one plant of bolt bacterium Trametes sp.LEF01, is preserved in on May 18th, 2015
China Committee for Culture Collection of Microorganisms's common micro-organisms center CGMCC, preserving number is CGMCC No.10489.The bolt
Bacterium CGMCC No.10489 fermentations can obtain in multi-enzyme system crude enzyme liquid, crude enzyme liquid laccase activity up to 4100.8U/L, cellulose
Enzyme enzyme activity is up to 2310U/L, and hemicellulase enzyme activity is up to 21271.8U/L, and pectase enzyme activity is up to 19800U/L.
It is first to pad bafta it is an object of the invention to provide a kind of bafta enzymatic scouring method that bolt bacterium produces crude enzyme liquid
A period of time is banked up in enzyme liquid, then insulation, is finally washed, go out enzyme.
Crude enzyme liquid containing bolt bacterium Trametes sp.LEF01 fermenting and producings in the enzyme liquid；The bacterium was May 18 in 2015
Day is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center CGMCC, and preserving number is CGMCC
No.10489。
The bolt bacterium CGMCC No.10489 fermentations can obtain multi-enzyme system crude enzyme liquid, and the crude enzyme liquid contains laccase, fiber
The compound enzyme system such as plain enzyme, hemicellulase, pectase.
In one embodiment of the invention, the crude enzyme liquid is the thick enzyme produced through sodium lignin sulfonate induction fermentation
Liquid.
In one embodiment of the invention, the preparation method of the crude enzyme liquid is：By bolt bacterium CGMCC No.10489
It is inoculated in potato fluid nutrient medium, shaken cultivation 5-7 days under conditions of 28-30 DEG C, 120-180rpm, centrifuges after activation
Supernatant is taken to produce crude enzyme liquid.Laccase activity 630.6U/L in obtained crude enzyme liquid, cellulose enzyme activity 1108.9U/L, hemicellulose
Plain enzyme enzyme activity 3844.9U/L, pectase enzyme activity 520.7U/L.
In one embodiment of the invention, lignosulfonates are also added with the potato fluid nutrient medium.
In one embodiment of the invention, the preparation method of the potato fluid nutrient medium：Formula per L, takes
Peeled potatoes 200g, stripping and slicing, boil, filter, then complementing to 1L, and add 20.0g glucose.
In one embodiment of the invention, the addition of the lignosulfonates is 0.8-1g.Obtained thick enzyme
Laccase activity 3080.8-4100.8U/L in liquid, cellulose enzyme activity 1910-2310U/L, hemicellulase enzyme activity 16271.8-
21271.8U/L, pectase enzyme activity 16800U-19800U/L.
In one embodiment of the invention, the consumption of crude enzyme liquid when padding is 1-5g/L, and treatment temperature is 30-
50℃。
In one embodiment of the invention, it is to be banked up at 20-30 DEG C 8-24 hours that the insulation, which is banked up,.
In one embodiment of the invention, the washing is washed in 90-95 DEG C, to remove the impurity for dezymotizing degraded,
And have concurrently and make enzyme deactivation.
In one embodiment of the invention, methods described also contains blanching step after washing, without cottonseed after bleaching
Shell is remained.
In one embodiment of the invention, methods described is specifically：(1) enzyme liquid is padded：Using bolt bacterium CGMCC
The crude enzyme liquid of No.10489 fermenting and producings, rolls 30-50 DEG C of enzyme temperature, crude enzyme liquid consumption 1-5g/L, bleeding agent 1-5g/L are rolled after enzyme
Fabric clot；(2) insulation is banked up：Clot fabric is banked up 8-24 hours at 20-30 DEG C, and fabric is slowly rotated during banking up；(3)
Washing：Fabric uncoiling is after 90-95 DEG C of washing, to remove the impurity for dezymotizing degraded, and have concurrently to make enzyme deactivation.
In one embodiment of the invention, solid-liquid ratio is 1 when methods described is padded：20-1：50.
In one embodiment of the invention, the bafta for through singing, desizing processing after pure cotton woven fabric.
In one embodiment of the invention, the pure cotton woven fabric is poplin cloth, yarn card, plain cloth.
Beneficial effects of the present invention：
(1) containing can Synergistic degradation cotton seed hulls a variety of enzyme components such as laccase, cellulase, hemicellulase, pectase,
Each enzyme has extraordinary compatibility, overcomes single enzyme and compounds brought poor compatibility problem；Scouring result is good, wetting
Property：The time drip less than 1s, whiteness more than 70%, strength loss is less than 5%, and COD value of waste water is the concise 10-20% of alkali；Through
Cloth cover is remained without cotton seed hulls after follow-up conventional bleaching.
(2) the enzymatic scouring effect of General Promotion bafta
(a) fiber is not damaged, fabric weightlessness reduction can improve bafta quality；
(b) fabrics feel soft, plentiful, cloth cover is glossy, without cotton seed hulls residual, clean mark；
(c) dyeability is improved, level-dyeing property is improved, tinctorial yield rises, dyeing defect rate declines to a great extent；
(d) to special sensitive fiber type it is concise more safety and reliability, such as natural color-cotton, cotton/hair, cotton/silk,
Cotton/fiber crops etc..
(3) it is energy-saving, reduce integrated cost
(a) energy consumption, water consume and sewage disposal expense are significantly reduced；
(b) COD value and wastewater flow rate of waste water are substantially reduced, cleanly production requirement is reached.
(c) operating environment safety, small to equipment damage.
Biomaterial preservation：
A kind of bolt bacterium, taxology is named as bolt bacterium Trametes sp., and China Microbiological is preserved on May 18th, 2015
Culture presevation administration committee common micro-organisms center CGMCC, preserving number is CGMCC No.10489, and preservation address is Beijing
The institute 3 of Chaoyang District North Star West Road 1.
Embodiment
(1) laccase activity
Definition：Enzyme amount needed for 1min internal oxiditions ABTS generates 1 μm of ol ABTS free radical is 1 enzyme-activity unit.
Assay method：It is that substrate determines paint with ABTS (2,2'- phenodiazines-bis- (3- ethyl-benzothiazole -6- sulfonic acid) ammonium salt)
Enzyme enzyme activity, that is, take 2mL 0.5mmol/L ABTS solution (to use pH5.0, concentration is 0.05mol/L Acetic acid-sodium acetate buffer solution
Prepare), blank sample is substituted with equivalent cushioning liquid, 37 DEG C of preheatings, is added 1mL crude enzyme liquids, is determined absorbance change at 420nm
Rate.Laccase activity=absorbance change rate × reaction solution volume × 1000 × extension rate/molar absorptivity number.
(2) cellulose enzyme activity
Definition：Enzyme amount needed for 1 μm of ol glucose is produced in 1min is 1 enzyme-activity unit.
Assay method：Cellulose enzyme activity is determined using DNS methods (3,5- dinitrosalicylic acid system), that is, takes the thick enzymes of 0.2mL
Liquid, add sodium carboxymethylcellulose (CMC-Na) solution of 1.8mL mass fractions 0.625% (with pH be 4.8,0.2mol/L acetic acid-
Sodium acetate buffer is prepared), blank sample adds equivalent NaAc_HAc buffer solution, reacts and adds immediately after 30min in 50 DEG C
Enter 2mL DNS reagents, boiling water bath 10min colour developings, cooling, dilution is settled to certain multiple, fully mixed, and determines and is inhaled at 550nm
Luminosity.Cellulose enzyme activity=concentration of glucose × reaction solution volume × 1000 × extension rate/reaction time.
(3) hemicellulase enzyme activity
Definition：Enzyme amount needed for 1 μm of ol xylose is produced in 1min is 1 enzyme-activity unit.
Assay method：Hemicellulase enzyme activity is determined using DNS methods, that is, takes 1mL crude enzyme liquids, adding 2mL mass fractions is
0.5% xylan suspension (using pH4.8, concentration 0.05mol/L NaAc_HAc buffer solution is prepared), blank sample adds
Enter equivalent NaAc_HAc buffer solution, 2mLDNS reagent terminating reactions, boiling water are added immediately after handling 30min at 60 DEG C
10min colour developings are bathed, room temperature is cooled to, dilution is settled to proper volume, absorbance at 540nm is determined after being well mixed.Hemicellulose
Plain enzyme enzyme activity computational methods are：Xylose concentration, hemicellulase enzyme activity=xylose concentration × anti-are obtained according to xylose standard curve
Liquid is answered to accumulate × 1000 × extension rate/reaction time.
(4) pectase enzyme activity
Definition：Polygalacturonic acid-catalyzed cleavage is set to produce the enzyme amount of the unsaturated galacturonic acids of 1 μm of ol in 1min.
Assay method：Pectase enzyme activity is determined by substrate of pectin, that is, takes 1mL crude enzyme liquids, the pectin of 2mL 0.2% is added
Glycine-NaOH buffer solution (pH9.4), blank sample adds equivalent pH9.4 Glycine-NaOH buffer solution, 45 DEG C
15min is reacted in water-bath, 0.03mol/L phosphoric acid 3mL is added, light absorption value at 235nm is determined.Pectase enzyme activity computational methods
For：Pectase enzyme activity=enzyme activity determination value × reaction solution volume × 1000 × extension rate/reaction time.
(5) assay method of wetability：Water (0.02mL) is dripped at water drop test length of normal cloth cover 1cm by determining, is remembered
The time that record water droplet is fully absorbed by cloth cover, 10 points of follow-on test, average on same sample.
Embodiment 1：The preparation method of bolt bacterium fermentation enzyme liquid
Peeled potatoes 200g is weighed, is cut into small pieces, the 1.0L that adds water boils 30min, 4 layers of filtered through gauze filter off potato
Block, 1.0L is complemented to by filtrate, adds 20.0g glucose, adds 0.8g lignosulfonates, fully dissolving.121 DEG C after packing
After autoclaving 20min, the Trametes of 5~6 days has been cultivated in cooling, sterile working, access on PDA slant mediums
The bacteria suspension of sp.LEF01 bacterial strains, shaken cultivation 6 days under the conditions of 28 DEG C in constant-temperature shaking incubator, rotating speed is 150rpm.Hair
Ferment terminates, zymotic fluid 4000rpm centrifugation 30min, takes supernatant to survey enzyme activity, laccase activity 3080.8U/L, cellulose enzyme activity
1910U/L, hemicellulase enzyme activity 16271.8U/L, pectase enzyme activity 16800U/L.
Embodiment 2：The bafta enzymatic scouring of crude enzyme liquid is produced based on bolt bacterium
Bafta is the pure cotton woven fabric such as poplin cloth, yarn card, plain cloth through singing, after desizing processing.
Carried out by following steps concise：
(1) enzyme liquid is padded：The bolt bacterium CGMCC No.10489 crude enzyme liquids obtained using the method according to embodiment 1, roll enzyme
30 DEG C of temperature, crude enzyme liquid consumption 5g/L, bleeding agent 1g/L, rolls fabric clot after enzyme；
(2) insulation is banked up：Clot fabric is banked up 8 hours at 30 DEG C, and fabric is slowly rotated during banking up；
(3) wash：Fabric uncoiling is after 95 DEG C of washings, to remove the impurity for dezymotizing degraded, and have concurrently to make enzyme deactivation.
Embodiment 3：The bafta enzymatic scouring of crude enzyme liquid is produced based on bolt bacterium
Bafta is the pure cotton woven fabric such as poplin cloth, yarn card, plain cloth through singing, after desizing processing.
Carried out by following steps concise：
(1) enzyme liquid is padded：The bolt bacterium CGMCC No.10489 crude enzyme liquids obtained using the method according to embodiment 1, roll enzyme
Temperature 50 C, crude enzyme liquid consumption 1g/L, bleeding agent 5g/L, rolls fabric clot after enzyme；
(2) insulation is banked up：Clot fabric is banked up 24 hours at 20 DEG C, and fabric is slowly rotated during banking up；
(3) wash：Fabric uncoiling is after 90 DEG C of washings, to remove the impurity for dezymotizing degraded, and have concurrently to make enzyme deactivation.
It is as shown in table 1 to the treatment effect of bafta using embodiment 2-3 method.
The bafta scouring result of table 1
Wherein, control 1 is traditional alkali process, and specific method is that alkali is concise：NaOH 25g/L, Na2SiO31g/L, 98 DEG C,
1h, bath raio 1: 20.
Control 2 is the effect using single enzyme (pectase) processing, pectin enzyme dosage 0.1g/L, pectase enzyme activity 170U/
L, pH value 8.5.Remaining condition is consistent with embodiment 2.
Control 3 is the effect using compound ferment treatment, and complex enzyme is cellulose enzyme activity 2000U/L, pectase enzyme activity
182U/L, remaining condition is consistent with embodiment 3.
In a word, as shown in Table 1, using the method for the present invention, scouring result is good, wetability：The time drip less than 1s, whiteness
More than 80%, strength loss is less than 5%, and COD value of waste water is the concise 10-20% of alkali；Cloth cover is without cotton after follow-up conventional bleaching
Seed shell is remained.
Although the present invention is disclosed as above with preferred embodiment, it is not limited to the present invention, any to be familiar with this skill
The people of art, without departing from the spirit and scope of the present invention, can do various changes and modification, therefore the protection model of the present invention
Enclose being defined of being defined by claims.
Claims (10)
1. a kind of bafta enzymatic scouring method, it is characterised in that methods described is that bafta is first padded into enzyme liquid, is then incubated heap
A period of time is put, is finally washed, go out enzyme；Thick enzyme containing bolt bacterium CGMCC No.10489 fermenting and producings in the enzyme liquid
Liquid；The bolt bacterium was preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on May 18th, 2015
CGMCC, preserving number is CGMCC No.10489.
2. according to the method described in claim 1, it is characterised in that crude enzyme liquid consumption when padding is 1-5g/L, processing temperature
Spend for 30-50 DEG C.
3. according to the method described in claim 1, it is characterised in that the crude enzyme liquid is similar through cotton seed hulls constituent structure
The crude enzyme liquid of thing induction fermentation production.
4. according to the method described in claim 1, it is characterised in that the preparation method of the crude enzyme liquid is：By bolt bacterium CGMCC
It is inoculated in after No.10489 activation in potato fluid nutrient medium, the shaken cultivation 5- under conditions of 28-30 DEG C, 120-180rpm
7 days, centrifuging and taking supernatant produced crude enzyme liquid.
5. method according to claim 4, it is characterised in that lignin is also added with the potato fluid nutrient medium
Sulfonate.
6. method according to claim 5, it is characterised in that the addition of the lignosulfonates is 0.8-1g.
7. according to the method described in claim 1, it is characterised in that methods described is specifically：(1) enzyme liquid is padded：Using bolt bacterium
The crude enzyme liquid of CGMCC No.10489 fermenting and producings, rolls 30-50 DEG C of enzyme temperature, the consumption 1-5g/L of crude enzyme liquid, bleeding agent 1-5g/
L, rolls fabric clot after enzyme；(2) insulation is banked up：Clot fabric is banked up 8-24 hours at 20-30 DEG C, and fabric is slow during banking up
Rotate；(3) wash：Fabric uncoiling is after 90-95 DEG C of washing, to remove the impurity for dezymotizing degraded, and have concurrently to make enzyme deactivation.
8. according to the method described in claim 1, it is characterised in that solid-liquid ratio is 1 when methods described is padded：20 to 1：50.
9. according to the method described in claim 1, it is characterised in that the bafta is the pure cotton through singing, after desizing processing
Woven fabric.
10. method according to claim 8, it is characterised in that the pure cotton woven fabric is poplin cloth, yarn card, plain cloth.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102146633A (en) * | 2011-01-15 | 2011-08-10 | 江南大学 | Method for scouring cotton fabric by applying cutinase and CBD (cellulose-binding domain)-alkaline pectinase |
| CN103669006A (en) * | 2012-09-14 | 2014-03-26 | 江南大学 | Method for performing biological pretreatment on cotton woven fabric by use of helicase |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IN2013MU03881A (en) * | 2013-12-12 | 2015-07-31 | Ajay Kumar Pillai |
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2015
- 2015-12-18 CN CN201510954133.7A patent/CN105401448B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102146633A (en) * | 2011-01-15 | 2011-08-10 | 江南大学 | Method for scouring cotton fabric by applying cutinase and CBD (cellulose-binding domain)-alkaline pectinase |
| CN103669006A (en) * | 2012-09-14 | 2014-03-26 | 江南大学 | Method for performing biological pretreatment on cotton woven fabric by use of helicase |
Non-Patent Citations (1)
| Title |
|---|
| 棉及棉毛混纺织物生物酶前处理研究;周民革;《中国优秀硕士论文全文数据库 工程科技I辑》;20091015;第B024-80页 * |
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Address after: 214122 Jiangsu city of Wuxi Province District Liangxi No. 898 South Road 7 layer beam Creek area of food science and Technology Park Patentee after: Jiangnan University Address before: 1800 No. 214122 Jiangsu city of Wuxi Province Li Lake Avenue Patentee before: Jiangnan University |
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