CN103669006B - A kind of method that utilizes helicase to carry out biological pretreatment method to cotton woven fabric - Google Patents

Abstract

Snailase is the compound enzyme extracted from Limax crop or digestive tract, mainly contains more than the 20 kind of enzymes such as cellulase, pectase, amylase, protease, is the by-product of Limax comprehensive utilization exploitation.But in the actual course of processing, the internal organs of Limax are often abandoned, which results in the biggest waste of enzyme resource.For Pretreatment of textile, pectase, amylase and the cellulase contained by Snailase is a kind of well compound enzyme system.This natural compound enzyme system of Snailase is applied in the biological refining process of cotton fabrics by the present invention, utilizes the hydrolysis of Snailase to remove pectin, waxiness and other impurity in cotton fiber, thus reaches to improve bafta wettability and the purpose of whiteness.Use the present invention to carry out concise to bafta, not only there is environmental protection and energy-conservation feature, and the by-product of Limax processing can be comprehensively utilized, meet the comprehensive development of the requirement of current sustainable development, beneficially dyeing and finishing industry and Limax processing of farm products.

Description

A kind of method that utilizes helicase to carry out biological pretreatment method to cotton woven fabric

technical field

The invention discloses a method for biological pretreatment of cotton woven fabrics by using helicase, which belongs to the technical field of cotton fabric dyeing and finishing pretreatment.

Background technique

In nature, snails mainly feed on leaves and shoots of plants. Therefore, during the long-term evolution process, biological enzymes that can digest various plant components have been produced in their bodies. In 1898, Biederman discovered that there are a large number of enzymes such as cellulase, hemicellulase, mannase and proteolytic enzyme in the digestive glands of snails. In 1922, Jiejia first isolated and extracted this mixed enzyme from the digestive gland of the snail, and called it "snail enzyme". In 1974, Chinese scientists also successfully separated and extracted helicase from the digestive glands of the brown cloud agate snail and the brown belt ringmouth snail respectively. At present, the industrial production of helicase has been preliminarily realized in China.

According to research reports, on average, 10-130mg of dry enzyme preparation can be extracted from every milliliter of snail digestive juice. Preliminary research shows that snail enzyme mainly contains more than 20 kinds of enzymes such as cellulase, pectinase, amylase and protease. Among them, cellulase and pectinase are the most abundant, and these two enzymes are the most commonly used enzyme preparations for the biological enzyme pretreatment of cotton fabrics. Among them, cellulase can remove impurities on the fiber and fluff on the surface of the fabric by hydrolyzing cellulose, and improve the hygroscopicity and smoothness of the fabric; pectinase can decompose the pectin contained in the cotton fiber and improve the wettability of the fabric. . In addition, other enzymes contained in snail enzymes, such as: hemicellulase, mannase and protease, etc., can work together with cellulase and pectinase, and will also play a role in the impurities on cotton fibers such as hemicellulose and protein. to a certain removal effect. Therefore, the use of helicase rich in various enzyme components in the biological enzyme pretreatment of cotton fabrics will produce a better scouring effect.

In addition, with the development of bio-enzyme technology in recent years, the bio-enzyme scouring of cotton fabrics has also developed from single-enzyme scouring to compound enzyme scouring. This is mainly due to the specificity of enzymes, a single enzyme can only remove certain impurities on the fabric, so the traditional single enzyme scouring has limited improvement in fabric performance, and the improvement of fabric performance is not comprehensive enough. Therefore, using complex enzyme system to refine fabrics has become an inevitable development trend of biological enzyme refining in the future. And helicase is just such a natural complex enzyme system that is in line with the refining of cotton fabrics. Therefore, the use of this enzyme to refine cotton fabrics not only has the characteristics of environmental protection and energy saving of biological treatment, but also can comprehensively utilize the by-products of snail processing, which is beneficial to the comprehensive development of dyeing and finishing industry and snail agricultural product processing.

Contents of the invention

The present invention introduces helicase, a natural compound scouring enzyme system, into the pretreatment process of cotton woven fabrics, and utilizes various biological enzyme components such as pectinase, cellulase, amylase and protease contained in helicase to improve the effect on cotton woven fabrics. The fabric gray cloth is subjected to biological pretreatment to remove impurities such as pectin, wax, starch slurry, lignin and pigment contained in the cotton fiber, and finally achieve the purpose of improving the wettability and whiteness of the cotton fabric. Adopting the invention can not only solve the problem of poor refining effect of a single enzyme, but also improve the comprehensive utilization rate of snail processing by-products and promote the sustainable development of the snail breeding industry.

Main solution of the present invention:

A process for biological pretreatment of cotton woven fabrics using helicase mainly adopts a semi-continuous pad-batch bio-enzyme refining method. The process flow is mainly composed of the following parts: the desized cotton woven fabric is firstly pretreated with hot water or low alkali, then the snail enzyme scouring solution is soaked and kept warm and piled up, and finally washed with hot water (also has the effect of killing enzymes), and kept at room temperature Washing, drying, and finally complete the biological pretreatment of cotton woven fabrics.

1. Cotton woven fabric pretreatment

Hot water pretreatment method: pretreat the desized cotton woven fabric in hot water at 90-100°C for 2-10 minutes, with a bath ratio of 1:50 (fabric weight: water volume). , washed with water at room temperature for 3 times, and then rolled dry (rolling rate 100%).

Low-alkali pretreatment method: pad the desized cotton woven fabric in lye at 90°C (lye formula: sodium hydroxide 10g/L, non-ionic surfactant 1g/L, two dipping and two padding , rolling rate 100%), time 2-5s, bath ratio 1:50, then high-temperature steaming at 120°C for 1-5min, and finally room temperature water washing 3 times, rolling dry (rolling rate 100%).

2. Snail enzyme refining

(1) Enzyme solution configuration: dissolve the solid helicase (helicase produced by Agate snail) with warm water at 40-50°C, then add it to the buffer prepared by disodium hydrogen phosphate and citric acid, and then Add 0-2g/L nonionic or anionic surfactant, stir well and filter. The concentration of the final prepared helicase refining solution is 2-10g/L, and the pH value is 4.5-6.5.

(2) Enzyme treatment: pad the pretreated fabric with snail enzyme solution at a speed of 10-20m/min. The ratio is 80%-100%, and it is stacked after rolling.

3. Insulation stacking

Temperature has an important influence on the scouring effect of enzymes. Cotton woven fabrics need to be stacked under unsaturated steam conditions of 50-65°C after padding with enzyme solution, and the time is between 30-90 minutes. The higher the temperature, the shorter the stacking time , the lower the temperature, the longer the stacking time.

4. Washing and drying

The stacked fabrics need to be washed once with hot water at about 95°C for about 5-20s. This process can not only remove most of the impurities treated with enzymes but also have a killing effect on snail enzymes, preventing enzymes from damaging cotton fabrics. Continue hydrolysis, after hot water washing, the fabric is washed 3 times with room temperature water, and finally dried.

detailed description:

The present invention is illustrated by the following examples, but the present invention is not limited to the following examples, and within the range not departing from the gist described before and after, changes and implementations are included in the technical scope of the present invention.

Example 1

Sample: Desized Cotton Poplin Fabric

a. Pretreatment: The pure cotton poplin after desizing is first treated in hot water at 95° C. for 10 minutes, with a liquor ratio of 1:50. After the treatment, it is washed with water at room temperature for 3 times, and then rolled dry (the scraping rate is 100%).

b. Pad the pretreated fabric with snail enzyme solution at a speed of 10m/min (enzyme concentration 5g/L, pH=6, temperature 50°C, level plus O concentration 2g/L), the padding method is double dipping Rolling, the excess rolling rate is 100%, stacking after rolling.

c. Stack the pure cotton poplin under the condition of unsaturated steam at 65°C for 45 minutes.

d. After stacking, wash once with hot water at 95°C for about 10 seconds, then wash three times with water at room temperature, and finally dry.

After the above treatment, the wetting time of pure cotton poplin was increased from 24s to 8s, and the whiteness value Wr was increased from 56.8 to 78.5.

Example 2

Sample: Desized Cotton Gauze Fabric

a. Pretreatment: Put the desized pure cotton yarn card in hot alkali at 90°C (lye formula: sodium hydroxide 10g/L, nonionic surfactant 1g/L, two dipping and two rolling, rate of 100%,) for padding, time 2s, bath ratio 1:50, then steamed at 120°C for 4 minutes at high temperature, and finally washed with water at room temperature for 3 times, and rolled dry (surplus rate 100%).

b. Pad the pretreated fabric with snail enzyme solution at a speed of 15m/min (enzyme concentration 5g/L, pH=6.5, temperature 50°C, level plus O concentration 1g/L), the padding method is double dipping Rolling, the excess rolling rate is 100%, stacking after rolling.

c. Stack the pure cotton poplin under the condition of unsaturated steam at 60°C for 30 minutes.

d. After stacking, wash once with hot water at 95°C for about 10 seconds, then wash three times with water at room temperature, and finally dry.

After the treatment by the above method, the wetting time of the pure cotton yarn card was increased from 20s before treatment to 3s, and the whiteness value Wr was increased from 58.5 to 82.3.

Example 3

Sample: Desized Cotton Khaki Fabric

a. Pretreatment: Put the pure cotton khaki after desizing in hot alkali at 90°C (lye formula: sodium hydroxide 10g/L, non-ionic surfactant 1g/L, two dipping and two rolling, the excess rate 100%,) padding, time 2s, bath ratio 1:50, then 120 ℃ high temperature steaming 3min, finally room temperature water washing 3 times, rolling dry (surplus rate 100%).

b. pad the pretreated fabric with snail enzyme solution (enzyme concentration 10g/L, pH=4.5, temperature 50°C, sodium dodecylbenzenesulfonate concentration 1g/L) at a speed of 10m/min, and pad The method is two-dip and two-rolling, and the excess rate of rolling is 100%, and it is stacked after rolling.

c. Stack the pure cotton khaki under the condition of unsaturated steam at 50°C for 60 minutes.

d. After stacking, wash once with hot water at 95°C for about 10 seconds, then wash three times with water at room temperature, and finally dry.

After the above treatment, the wetting time of pure cotton khaki was increased from 15s to 3.5s, and the whiteness value Wr was increased from 60.5 to 80.3.

Claims (4)

1. utilizing the method that Snailase carries out Bio-Pretreatment to cotton fabrics, its technological process includes the following aspects:

(1) pretreatment: the cotton fabrics after desizing carries out pretreatment initially with 90-100 DEG C of hot water or 10g/L concentration Caustic soda；

(2) ferment treatment: pretreated cotton fabrics is padded Snailase liquid, the mode of padding is that two leachings two are rolled, and pads the temperature for the treatment of fluid Degree is for room temperature to 65 DEG C, and pick-up is 80%-100%；

(3) insulation is banked up: bank up under the conditions of 50-65 DEG C of unsaturated steam after padding enzyme liquid, time 30-90min；

(4) washing and dry: after having banked up, cotton fabrics need to be first by the hot wash 1 time of 95 DEG C, time 5-20s, hot water wash After washing, cotton fabrics is washed 3 times by room temperature again, finally dries.

A kind of method utilizing Snailase that cotton fabrics is carried out Bio-Pretreatment the most according to claim 1, it is characterised in that adopt Enzyme be Snailase, the source of this enzyme is escargot (snails), China Limax, flower garden Limax and Achates Limax.

A kind of method utilizing Snailase that cotton fabrics is carried out Bio-Pretreatment the most according to claim 1, it is characterised in that should The cotton fabrics that technique is suitable for includes plain cloth, yarn card, poplin cloth and khaki.

A kind of method utilizing Snailase that cotton fabrics is carried out Bio-Pretreatment the most according to claim 1, it is characterised in that should The Snailase liquid that method uses consists of: Snailase concentration 2-10g/L, nonionic or anionic surfactant concentration are 0-2g/L, buffer is disodium hydrogen phosphate and citric acid systems, and pH value is between 4.5-6.5.