CN103667362A - 一种利用木质纤维素原料高温同步糖化发酵生产乙醇的新工艺 - Google Patents
一种利用木质纤维素原料高温同步糖化发酵生产乙醇的新工艺 Download PDFInfo
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- CN103667362A CN103667362A CN201210362282.0A CN201210362282A CN103667362A CN 103667362 A CN103667362 A CN 103667362A CN 201210362282 A CN201210362282 A CN 201210362282A CN 103667362 A CN103667362 A CN 103667362A
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- fermentation
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- ethanol
- saccharification
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title claims abstract description 101
- 238000000034 method Methods 0.000 title claims abstract description 30
- 230000008569 process Effects 0.000 title claims abstract description 21
- 239000002994 raw material Substances 0.000 title claims abstract description 21
- 230000001360 synchronised effect Effects 0.000 title claims abstract description 12
- 230000002478 diastatic effect Effects 0.000 title 1
- 235000019441 ethanol Nutrition 0.000 title 1
- 238000000855 fermentation Methods 0.000 claims abstract description 50
- 230000004151 fermentation Effects 0.000 claims abstract description 50
- 239000000758 substrate Substances 0.000 claims abstract description 24
- 108010059892 Cellulase Proteins 0.000 claims abstract description 19
- 229940106157 cellulase Drugs 0.000 claims abstract description 19
- 230000007071 enzymatic hydrolysis Effects 0.000 claims abstract description 9
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims abstract description 9
- 238000004519 manufacturing process Methods 0.000 claims abstract description 9
- 239000007787 solid Substances 0.000 claims abstract description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract 3
- 229910052799 carbon Inorganic materials 0.000 claims abstract 3
- 239000008103 glucose Substances 0.000 claims description 16
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 14
- 239000001888 Peptone Substances 0.000 claims description 8
- 108010080698 Peptones Proteins 0.000 claims description 8
- 235000019319 peptone Nutrition 0.000 claims description 8
- 229940041514 candida albicans extract Drugs 0.000 claims description 7
- 239000012138 yeast extract Substances 0.000 claims description 7
- 240000008042 Zea mays Species 0.000 claims description 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 4
- 230000001580 bacterial effect Effects 0.000 claims description 4
- 235000005822 corn Nutrition 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 4
- 239000002028 Biomass Substances 0.000 claims description 2
- 235000015278 beef Nutrition 0.000 claims description 2
- 239000000284 extract Substances 0.000 claims description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims 4
- 229910052757 nitrogen Inorganic materials 0.000 claims 2
- 235000017060 Arachis glabrata Nutrition 0.000 claims 1
- 244000105624 Arachis hypogaea Species 0.000 claims 1
- 235000010777 Arachis hypogaea Nutrition 0.000 claims 1
- 235000018262 Arachis monticola Nutrition 0.000 claims 1
- 244000046052 Phaseolus vulgaris Species 0.000 claims 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims 1
- 235000019764 Soybean Meal Nutrition 0.000 claims 1
- 229920002472 Starch Polymers 0.000 claims 1
- 229930006000 Sucrose Natural products 0.000 claims 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims 1
- 238000012262 fermentative production Methods 0.000 claims 1
- 235000013312 flour Nutrition 0.000 claims 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 claims 1
- 235000012054 meals Nutrition 0.000 claims 1
- 235000013379 molasses Nutrition 0.000 claims 1
- 235000020232 peanut Nutrition 0.000 claims 1
- 239000004455 soybean meal Substances 0.000 claims 1
- 239000008107 starch Substances 0.000 claims 1
- 235000019698 starch Nutrition 0.000 claims 1
- 239000005720 sucrose Substances 0.000 claims 1
- 229940088594 vitamin Drugs 0.000 claims 1
- 229930003231 vitamin Natural products 0.000 claims 1
- 235000013343 vitamin Nutrition 0.000 claims 1
- 239000011782 vitamin Substances 0.000 claims 1
- 150000003722 vitamin derivatives Chemical class 0.000 claims 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract description 23
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 abstract description 23
- 238000002474 experimental method Methods 0.000 abstract description 7
- 239000000446 fuel Substances 0.000 abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 31
- 108090000790 Enzymes Proteins 0.000 description 22
- 102000004190 Enzymes Human genes 0.000 description 22
- 229940088598 enzyme Drugs 0.000 description 22
- 239000000463 material Substances 0.000 description 13
- 239000002609 medium Substances 0.000 description 13
- 238000006243 chemical reaction Methods 0.000 description 9
- 238000002360 preparation method Methods 0.000 description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- 239000001913 cellulose Substances 0.000 description 6
- 229920002678 cellulose Polymers 0.000 description 6
- 238000003756 stirring Methods 0.000 description 6
- GUBGYTABKSRVRQ-CUHNMECISA-N D-Cellobiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-CUHNMECISA-N 0.000 description 5
- 238000012546 transfer Methods 0.000 description 5
- 238000009423 ventilation Methods 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 241000282414 Homo sapiens Species 0.000 description 4
- HYBBIBNJHNGZAN-UHFFFAOYSA-N furfural Chemical compound O=CC1=CC=CO1 HYBBIBNJHNGZAN-UHFFFAOYSA-N 0.000 description 4
- 238000004128 high performance liquid chromatography Methods 0.000 description 4
- 230000005764 inhibitory process Effects 0.000 description 4
- 108010047754 beta-Glucosidase Proteins 0.000 description 3
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- 238000009776 industrial production Methods 0.000 description 3
- 239000002054 inoculum Substances 0.000 description 3
- 239000007222 ypd medium Substances 0.000 description 3
- NOEGNKMFWQHSLB-UHFFFAOYSA-N 5-hydroxymethylfurfural Chemical compound OCC1=CC=C(C=O)O1 NOEGNKMFWQHSLB-UHFFFAOYSA-N 0.000 description 2
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
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- 238000003912 environmental pollution Methods 0.000 description 2
- 239000003966 growth inhibitor Substances 0.000 description 2
- RJGBSYZFOCAGQY-UHFFFAOYSA-N hydroxymethylfurfural Natural products COC1=CC=C(C=O)O1 RJGBSYZFOCAGQY-UHFFFAOYSA-N 0.000 description 2
- 239000013067 intermediate product Substances 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 230000035484 reaction time Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 239000010902 straw Substances 0.000 description 2
- 230000009469 supplementation Effects 0.000 description 2
- 230000001502 supplementing effect Effects 0.000 description 2
- 241000499912 Trichoderma reesei Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000005202 decontamination Methods 0.000 description 1
- 230000003588 decontaminative effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000009229 glucose formation Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
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- 238000011218 seed culture Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
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CN201210362282.0A CN103667362A (zh) | 2012-09-21 | 2012-09-21 | 一种利用木质纤维素原料高温同步糖化发酵生产乙醇的新工艺 |
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CN201210362282.0A CN103667362A (zh) | 2012-09-21 | 2012-09-21 | 一种利用木质纤维素原料高温同步糖化发酵生产乙醇的新工艺 |
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CN103667362A true CN103667362A (zh) | 2014-03-26 |
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CN201210362282.0A Pending CN103667362A (zh) | 2012-09-21 | 2012-09-21 | 一种利用木质纤维素原料高温同步糖化发酵生产乙醇的新工艺 |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105624208A (zh) * | 2016-03-30 | 2016-06-01 | 辽宁石油化工大学 | 一种木质纤维素高温酶解发酵产乙醇的方法 |
CN105671090A (zh) * | 2016-03-30 | 2016-06-15 | 辽宁石油化工大学 | 一种木质纤维素高温酶解发酵产乙醇的方法 |
CN109642244A (zh) * | 2016-06-16 | 2019-04-16 | 波特研究公司 | 基于淀粉的增殖微生物的方法和系统 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102041235A (zh) * | 2009-10-21 | 2011-05-04 | 中国石油化工股份有限公司 | 一株耐高温酿酒酵母菌及其应用 |
CN103374532A (zh) * | 2012-04-24 | 2013-10-30 | 天津工业生物技术研究所 | 一株生产乙醇的耐高温酵母菌及其应用 |
-
2012
- 2012-09-21 CN CN201210362282.0A patent/CN103667362A/zh active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102041235A (zh) * | 2009-10-21 | 2011-05-04 | 中国石油化工股份有限公司 | 一株耐高温酿酒酵母菌及其应用 |
CN103374532A (zh) * | 2012-04-24 | 2013-10-30 | 天津工业生物技术研究所 | 一株生产乙醇的耐高温酵母菌及其应用 |
Non-Patent Citations (1)
Title |
---|
田沈 等: "一株酵母菌高温驯化与同步糖化发酵研究", 《太阳能学报》 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105624208A (zh) * | 2016-03-30 | 2016-06-01 | 辽宁石油化工大学 | 一种木质纤维素高温酶解发酵产乙醇的方法 |
CN105671090A (zh) * | 2016-03-30 | 2016-06-15 | 辽宁石油化工大学 | 一种木质纤维素高温酶解发酵产乙醇的方法 |
CN109642244A (zh) * | 2016-06-16 | 2019-04-16 | 波特研究公司 | 基于淀粉的增殖微生物的方法和系统 |
US11959119B2 (en) | 2016-06-16 | 2024-04-16 | Poet Research, Inc. | Methods and systems for starch based propagation of a microorganism |
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Owner name: TIANJIN INSTITUTE OF INDUSTRIAL BIOTECHNOLOGY, CHI Free format text: FORMER OWNER: TIANJIN INSTITUTE OF INDUSTRIAL BIOTECHNOLOGY Effective date: 20140319 |
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Effective date of registration: 20140319 Address after: 300308 Tianjin Airport Economic Zone seven West Road No. 32 Applicant after: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences Address before: 300308 Tianjin Airport Economic Zone seven West Road No. 32 Applicant before: Tianjin Institute of Industrial Biotechnology |
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