CN103657548A - Method and equipment for automatically adding liquid reagent - Google Patents
Method and equipment for automatically adding liquid reagent Download PDFInfo
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- CN103657548A CN103657548A CN201210317128.1A CN201210317128A CN103657548A CN 103657548 A CN103657548 A CN 103657548A CN 201210317128 A CN201210317128 A CN 201210317128A CN 103657548 A CN103657548 A CN 103657548A
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Abstract
The invention relates to a method and equipment for automatically adding a liquid reagent. The method comprises the following steps: automatically adding the liquid reagent to at least one solid phase substrate material, wherein the liquid reagent is positioned in a chamber; calculating a residual amount x of the liquid reagent in the chamber; comparing the residual amount x of the liquid reagent with a reagent amount a needed by the solid phase substrate material to obtain a comparison result; determining whether continuing to add the liquid reagent to the solid phase substrate material according to the comparison result. Through the method and the equipment provided by the invention, the equipment for automatically adding liquid can be applied to practical production, so that the full-automatic function is realized, the production efficiency is improved, and meanwhile the production cost is reduced.
Description
Technical field
The present invention relates to a kind of method and equipment of automatic liquid feeding body reagent, particularly add the method and apparatus of micro liquid reagent.
Background technology
Whether utilize immune association reaction principle to detect in sample exists this technology of analyte to be widely used in every field.Can carry out monitoring of diseases and the mankind's health status (early pregnancy, tumour, infectious disease, drugs etc.) with the analyte that it detects in various biological specimens (saliva, blood, urine, serum, sweat etc.).The cardinal principle of this detection technique is to be based upon the performance between immune molecule with specific bond, for example antibody and antigen, haptens/antibody, biotin and antibiotin etc.In addition, a lot of such detections can complete on solid matrix material, conventional lateral flow solid-phase matrix material for example, and in glass or plastics porous disc, device for immunochromatography etc.How need to detect relevant material and be fixed on solid dielectric, in a lot of open files, describing, such as: US7736591B2 discloses a kind of liquid that adds to the method and apparatus on mounting medium.By this method and equipment, the material that analyte in sample can be combined is added on one or more solid dielectrics, in lateral flow test strips.But, in practical operation, there is following problem:
1. solution is housed in the cavity of liquid reagent automatically and while being added continuously on a plurality of solid-phase matrix materials on equipment, after the solution of a cavity adds, can just in time certain whole piece solid-phase matrix Material Addition can not be finished at every turn; And again fill it up with after solution when cavity, due to the design limitations of equipment, will be from initial position, namely one end of a new solid-phase matrix material starts to add.Like this, the previous solid-phase matrix material not adding just cannot continue to add, and will cause scrapping of this whole solid-phase matrix material.
2. when equipment Halfway Stopping in operation, then while starting to operate, equipment will start operation from initial position again, there will be equally the situation of problem 1.
Conventionally, for fear of above-mentioned problem, this equipment all can need manual operation when operation, by visually observing the liquid reagent amount in cavity, and judges the whole solid-phase matrix material of enough interpolations one, and artificial shut-down operation.And after each Halfway Stopping operation, when start operation next time again, first liquid in cavity is filled it up with at initial end resume operations, cannot be realized real automatic interpolation function, both reduced operating efficiency, improved again production cost.
Summary of the invention
[0003] the invention provides a kind of method of automatic liquid feeding body reagent, after addition by liquid reagent surplus and a solid-phase matrix material require in comparison cavity, automatically determine whether to continue add next solid-phase matrix material or stop adding solid-phase matrix material, and automatically liquid in cavity is supplemented full, can make to add the real function of automatically adding that realizes of equipment of liquid, reduce running cost, enhance productivity.
On the one hand, the method of the automatic liquid feeding body reagent in the present invention, comprise the following steps: liquid reagent automatic powder adding is added at least one solid-phase matrix material, wherein, liquid reagent is arranged in a cavity, calculates the liquid reagent surplus x in this cavity, allows the amount of reagent a of liquid reagent surplus x and a solid-phase matrix material require compare, draw comparative result, according to comparative result, judge whether to continue to add liquid reagent to solid-phase matrix material.
In a preferred mode, when liquid reagent surplus x is more than or equal to the amount of reagent a of a solid-phase matrix material require, liquid reagent is continued to add on next solid-phase matrix material.In another optimal way, when liquid reagent surplus x is less than the amount of reagent a of a solid-phase matrix material require, stop adding liquid reagent to next solid-phase matrix material.More preferred, allow liquid reagent be full of described cavity.
In more preferred modes, x can be obtained to a numerical value divided by a value, when this numerical value is Integer n or non-integer n.m, n>=0, m>0, add liquid reagent and stop adding after n solid-phase matrix material, then allow liquid reagent be full of described cavity.
In some embodiments, when the comparative result of x and a is na<=x< (n+1) a, wherein n is integer, n>=0, continue to add liquid reagent and stop adding after n solid-phase matrix material, then allow liquid reagent be full of described cavity.In other embodiments, when the comparative result of x and a is x=(n+1) a, wherein n is integer, and n>=0 continues to add liquid reagent and stops adding after n+1 solid-phase matrix material, then allows liquid reagent be full of described cavity.
In a preferred mode, by a computing element, compare x and a, and draw comparative result.More specifically, computing element judges whether to continue to add liquid reagent to solid-phase matrix material by comparative result.
In other modes, by computing element, calculate cavity liquid reagent surplus x, x=cavity total capacity y-na, wherein, n is solid-phase matrix material quantity, n>=0.Or, in another embodiment, the liquid reagent amount z that liquid reagent surplus x=cavity total capacity y-has added.
In some embodiments, the test strips that solid-phase matrix material is immunoassay.In other modes, a plurality of reagent receives bar and is positioned in an agent plate, in an agent plate, a plurality of reagent strips, consists of.
In some concrete embodiments, liquid reagent is the gold mark solution for immunodiagnosis, and this liquid reagent automatically adding is gold mark solution.
On the other hand, the present invention also provides a kind of equipment of automatic liquid feeding body reagent, comprises a cavity, and this cavity is for receiving fluids reagent; Wherein, this equipment also comprises computing element, by the liquid reagent surplus x in this computing element comparison cavity and the liquid reagent requirement a of a solid-phase matrix material, and draws comparative result.
In preferred mode, this computing element judges whether to continue to add liquid reagent to solid-phase matrix material by comparative result.More specifically, in mode, computing element feeds back to this equipment by judged result.
In some embodiments, when the comparative result that computing element obtains is x>=a, this equipment continues to add liquid reagent to next solid-phase matrix material.In other embodiments, when the comparative result that computing element obtains is x<a, this equipment stops adding liquid reagent to next solid-phase matrix material; And this equipment is full of cavity by liquid reagent.
In a preferred mode, the manner of comparison of computing element is the mode of x/a, the comparative result as x and a is: x/a=n (integer), or x/a=n.m(non-integer), this equipment continues to add liquid reagent and stops adding after n solid-phase matrix material, then liquid reagent is full of to described cavity.
In another embodiment, when the comparative result that computing element obtains is na<=x< (n+1) a, wherein n is integer, n>=0, this equipment continues to add liquid reagent and stops adding after n solid-phase matrix material, then liquid reagent is full of to cavity.Or, in another mode, when the comparative result that computing element obtains is x=(n+1) a, wherein n is integer, n>=0, this equipment continues to add liquid reagent and stops adding after n+1 solid-phase matrix material, then liquid reagent is full of to cavity.
One preferred embodiment in, computing element can calculate the liquid reagent surplus x in cavity.Specifically, x=cavity total capacity y-na; Wherein, n is solid-phase matrix material quantity, n>=0.Or in another embodiment, the mode of calculating liquid reagent surplus x is: the liquid reagent amount z that x=cavity total capacity y-has added.
In some embodiment, this equipment also comprises the shower nozzle being connected with cavity one end, the driver and the controller that are connected with the cavity other end.
One more specifically in embodiment, described computing element is connected with controller.
In other embodiment, the equipment of this automatic liquid feeding body reagent also comprises a fluid reservoir, for storing liquid reagent.And this equipment can make the liquid reagent of fluid reservoir move in the cavity of receiving fluids reagent.
beneficial effect
by method and apparatus of the present invention, the equipment of automatic liquid feeding body can be applied in actual production, realize automation function completely, enhance productivity, reduce production costs simultaneously.
Accompanying drawing explanation
Fig. 1 is the equipment schematic diagram of traditional automatic liquid feeding body;
Fig. 2 is the equipment schematic diagram of automatic liquid feeding body of the present invention;
Fig. 3 is cavity and middle liquid reagent surplus view (during x>a) thereof in present device;
Fig. 4 is cavity and middle another view of liquid reagent surplus (during x=a) thereof in present device
Fig. 5 is cavity and middle another view of liquid reagent surplus (during x<a) thereof in present device;
Fig. 6 is the nozzle motion path schematic diagram of automatic liquid feeding body equipment.
Fig. 7 is that the nozzle of automatic liquid feeding body equipment is got back to the schematic diagram of initial end by terminal.
Description of reference numerals:
Solid-phase matrix material (test strips) 175, shower nozzle 128, the nozzle 205 of shower nozzle, the movable valve 204 of shower nozzle, liquid outlet channel 152, controlled valve 145, feed pathway 150, liquid storage chamber 116, liquid reagent 130, piston 118, syringe pump shell 162, syringe pump (cavity of receiving fluids reagent) 120, driver 142, controller 114, computing element 113, the equipment 108 of traditional automatic liquid feeding body, the equipment 110 of automatic liquid feeding body of the present invention, the liquid reagent amount a 300 of a solid-phase matrix material require, the first circuit 401, the second circuit 402, tertiary circuit 403, the 4th circuit 404, the 5th circuit 405, shower nozzle is gone back to the path 500 of starting point by terminal
The specific embodiment
Below the structure the present invention relates to or these technical terms using are described further.
In the following detailed description, the subsidiary reference word of legend is a part here, and it illustrates to illustrate the mode that the present invention can actable specific concrete scheme.We do not get rid of the present invention and can also carry out other concrete scheme and without prejudice to the scope of application of the present invention in the situation that, change structure of the present invention.
As shown in Figure 1, be the equipment 108 of an existing traditional automatic liquid feeding body reagent, this equipment is by adding the liquid reagent in liquid storeroom 116 to syringe pump 120 and filling it up with in syringe pump 120, again by syringe pump 120 by continuous the adding in a plurality of solid-phase matrix materials or test strips 175 of liquid reagent, with complete by liquid reagent quantitative add the operation on solid-phase matrix material 175 to.When equipment has just started to operate, when in syringe pump 120, liquid reagent 130 is not the state being full of, first allow liquid reagent by feed pathway 150 and valve 145, add in syringe pump 120 from storeroom 116, make syringe pump 120 be full of liquid reagent, then controller 114 is controlled drivers 142 and is come driven plunger 118 allow to be full of the syringe pump 120 of liquid reagent that liquid is discharged, the liquid of discharging arrives shower nozzle 128 by liquid outlet channel 152, again by the valve 204 in shower nozzle and nozzle 205 by quantitative being added continuously on one or more solid matrix materials 175 of liquid reagent.Wherein, shower nozzle 128 is connected with controller 114, controller 114 is controlled the motion path of shower nozzle and nozzle, in a specific embodiment, as shown in Figure 6, while starting to add liquid, nozzle 205 is by a fixing initial position A, the initial end A of the first circuit 401 starts to add liquid and arrives after this circuit terminal B, stop adding liquid reagent and directly move to the second circuit 402 starting point C ends, continuing to start to add liquid reagent holds to the second circuit terminal D, stop adding liquid reagent and by D, hold the initiating terminal E place that moves to tertiary circuit 403 to start to add again, arrive after tertiary circuit terminal F, stop adding liquid reagent and move to G end, the initiating terminal of the 4th circuit 404 starts to add liquid reagent, until arrive the terminal H of the 4th circuit, stop adding liquid reagent, then from H, hold the starting point I end that directly moves to the 5th circuit 405 to start to add liquid reagent, the J that finally reaches home end.When nozzle 205 is positioned at after the 5th circuit terminal J point, stop adding liquid reagent and directly get back to initiating terminal A point by J point, as shown in arrow in Fig. 7 500, then start the automatic interpolation of next round.In practical operation, by transport tape, solid matrix material 175 is sent on 5 circuits above, by this equipment 108, automatically added, and conveyer belt is by ceaselessly moving, the solid matrix material 175 having added is seen off, and un-added host material 175 continuous supplementation are added in the interpolation circuit of nozzle 205.Wherein, the integral multiple of the amount of liquid of the host material that not necessarily will add because of a chamber liquid capacity of syringe pump 120, do not having in manually-operated situation, when the liquid reagent in syringe pump 120 has been added, there will be nozzle 205 to arrive the centre of a certain circuit, such as, be positioned at the E end of tertiary circuit 403 and the centre that F holds.And after in equipment 108 automatic detection discovery cavitys (syringe pump) there there is no liquid, start and add the liquid of fluid reservoir 116 to program in cavity 120, meanwhile, nozzle 205 is returned to initial position, i.e. A end.Like this, the solid-phase matrix material being positioned on tertiary circuit is not just scrapped because adding complete.In another embodiment; after a collection of solid-phase matrix Material Addition completes; the liquid reagent that also has in syringe pump also has residue; or the liquid during because of disorderly closedown in syringe pump has residue; before starting the operation of next group host material, this equipment starts after can again liquid reagent in syringe pump being full of again.Like this, just reduced operating efficiency.Therefore, the equipment 108 of traditional automatic liquid feeding body all has problems in production efficiency or on production cost.
In the present invention, the annex solution body method of automation is provided, pass through the method, can go out the whole solid-phase matrix material of the enough interpolations one of liquid reagent surplus in cavity by automatic decision, and take corresponding step to avoid liquid in cavity to be added the light time, and the situation that a whole solid-phase matrix material has not added.Can really realize the full automation of the equipment 110 of automatic liquid feeding body, save human cost, improve production efficiency, meanwhile, also eliminate scrapping of solid-phase matrix material, save cost.
The method is used in the process of adding at liquid, when the liquid reagent that is positioned at cavity 120 is added continuously on a plurality of solid-phase matrix materials 175, start to calculate the liquid reagent surplus x in syringe pump 120 simultaneously, by the amount of reagent a(300 of the liquid reagent surplus x calculating and a solid-phase matrix material require) compare, draw comparative result, then, according to comparative result, judge whether syringe pump 120 continues to add liquid reagent to next solid-phase matrix material 175.Wherein, when syringe pump 120 is full of liquid reagent, liquid reagent surplus equals syringe pump total amount of liquid y.In addition,, for a solid-phase matrix material, amount of reagent a is fixed value.When each solid-phase matrix material is different, the amount of reagent a needing also may ensue variation.
More specifically, in embodiment, when the liquid reagent surplus x in comparative result is cavity is more than or equal to the amount of reagent a of a solid-phase matrix material require, continue liquid reagent to add on next solid-phase matrix material.The liquid reagent amount 300 of a solid-phase matrix material require is a, and unit is ml.The surplus x ml of liquid 130 in syringe pump, when x is more than or equal to a, this equipment continues running, liquid reagent in syringe pump 120 130 is continued to add on next solid-phase matrix material, Fig. 3 is the schematic diagram that the liquid reagent surplus 130 in cavity is greater than the amount of reagent a that host material needs, and liquid reagent surplus 130 in Fig. 4 cavity equals the schematic diagram of the amount of reagent a that a host material needs.
In another specific embodiment, when comparative result is liquid reagent surplus x while being less than the amount of reagent a of a solid-phase matrix material require, the interpolation of stop liquid reagent.As shown in Figure 5, when in syringe pump 120, the surplus x of liquid 130 is less than the liquid reagent amount a of a solid-phase matrix material require, because remaining liquid can not complete a solid-phase matrix Material Addition, when in syringe pump 120, liquid exhausts, one whole solid-phase matrix material can not all be added by liquid reagent, can cause like this this host material 175 to be scrapped, so stop adding liquid.More specifically, in mode, when liquid reagent surplus x is less than the amount of reagent a of a solid-phase matrix material require, will in the cavity of receiving fluids reagent, be full of liquid reagent.Stopping adding liquid reagent after host material, will in syringe pump 120, be full of liquid reagent, then the liquid reagent that carries out next round adds the operation of host material to, thereby realize full-automatic cycling.
Therefore according to said method, after the complete solid-phase matrix material 175 of the every interpolation of liquid reagent, liquid reagent surplus x in the cavity of receiving fluids reagent is calculated in capital again, and a solid-phase matrix material requirement a of surplus x and interpolation is compared, and by comparative result, judge next step operating procedure: when x>=a, continue to add liquid reagent to next solid-phase matrix material; When x<a, stop adding liquid reagent to next solid-phase matrix material, meanwhile, liquid reagent is supplemented and also supplements full into the cavity of receiving fluids reagent.Then, carry out the automatic interpolation of next round and calculate relatively.Like this, when this method of automatically adding has been avoided prosthetic operation, equipment is when in cavity, amount of liquid adds a whole solid-phase matrix material not and continue to add and cause scrapping of host material, and can make the equipment automatically adding realize real full-automatic interpolation function.
In some embodiment, relatively time, by liquid reagent surplus x is drawn to a numerical value P divided by a host material requirement a, when this numerical value P is Integer n, such as n=27, add the interpolation of stop liquid after 27 host materials, now in cavity, amount of liquid is 0, be in cavity, there is no liquid, then will in cavity, be full of the operation of carrying out next round after liquid.And when P is non-integer n.m, such as P=35.6, or P=35.58 etc., add the interpolation of stop liquid after 35 host materials, now in cavity, remaining amount of liquid x is less than a, then will in cavity, be full of the operation of carrying out next round after liquid reagent.
In other specific embodiments, when the liquid reagent amount a of the liquid reagent surplus x in cavity and solid-phase matrix material require compares, when comparative result is na<=x< (n+1) a, wherein n is integer, and n>=0, add after n solid-phase matrix material, will in cavity, be full of liquid reagent.That is to say, when in cavity, liquid reagent surplus x is at na and (n+1) between a when identical with na (comprise x), can add n solid-phase matrix material, after having added, now in cavity, remaining amount of liquid x is less than a or has been 0, therefore, stop continuing to add, and will in cavity, be full of liquid reagent; Then carry out next round cycling then.And when comparative result is x=(n+1) a, wherein n is integer, n>=0, add after n+1 solid-phase matrix material, now in cavity, liquid is all emptying, and x=0, carries out next round cycling again by being full of liquid reagent in cavity 120.For instance, the liquid reagent amount needing when solid-phase matrix material of interpolation 175 is 0.15ml, and the total capacity of syringe pump (cavity) is 10ml, in a kind of situation, in syringe pump, be full of solution, x=y=10ml, a=0.15ml, x is between 66a and 67a, after allowing syringe pump add 66 solid-phase matrix materials, stop continuing to add liquid to solid-phase matrix material, subsequently, syringe pump is full of for after 10ml, again calculates and add liquid to solid-phase matrix material.In another kind of situation, while starting to operate, in syringe pump, remaining amount of liquid x is less than y value 10ml, the equipment of this automatic liquid feeding body reagent is when starting to operate, syringe pump 120 is not at initial position, that is to say that piston is positioned at the somewhere of syringe pump 120, such as, x value is 6ml, and a value is constant, be still 0.15ml, x equals 40a, after now allowing syringe pump 120 add 40 solid-phase matrix materials, stop continuing to add liquid to solid-phase matrix material, subsequently, syringe pump 120 is full of to liquid reagent, be 10ml, again calculate and add liquid reagent to the operation of solid-phase matrix material.
In some specific embodiments, can calculate liquid reagent surplus x, liquid reagent surplus x=cavity total capacity y-na by a computing element 113; Wherein, n is solid-phase matrix material quantity, n>=0.Now, computing element stores total capacity y and a solid matrix material require amount of liquid a of cavity (syringe pump) in advance, computing element, by recording after the frequency n (that is to say solid-phase matrix material quantity) of syringe pump 120 interpolations, calculates liquid reagent surplus x in syringe pump 120 according to the program formula x=y-na setting.In other embodiment, syringe pump 120 liquid reagent surplus x pass through formula: the liquid reagent amount z that liquid reagent surplus x=cavity total capacity y-has added.Wherein, the total amount of liquid z having added is by computing element real time record.The mode of liquid reagent surplus in this calculating syringe pump (cavity) 120, because the amount of liquid z having added is real time record, so a very large advantage is exactly to calculate in real time surplus x, namely has up-to-date x value all the time.Like this, even if utilize the equipment of this method cut in operation, and then while continuing operation, x value is also accurately.This just guarantees when follow-up computing element 113 compares x and a value can not make a mistake.
In other specific embodiments, can be by the liquid reagent surplus x in computing element 113 comparison cavitys and the amount of reagent a of a solid-phase matrix material require.After obtaining liquid reagent surplus x, compare with a value being arranged in advance in computing element 113, thereby obtain the result that x is greater than a or is less than a.More specifically, in embodiment, further by computing element 113, judge whether to continue to add liquid, and judged result is fed back to equipment.When computing element 113 calculates x and is more than or equal to a, judge and need to continue to add liquid reagent, then, computing element 113 is given this result feedback the equipment automatically adding.Same, when computing element 113 comparative results are x while being less than a, by computing element 113, judge and need to stop adding liquid, and the liquid in syringe pump 120 need to be full of.This information is fed back to automatic powder adding oil (gas) filling device 110 equally, the instruction that automatic powder adding oil (gas) filling device 110 can be carried out halt instruction and fill it up with syringe pump 120 like this.
Certainly, the mode of the amount of reagent a of computing element 113 comparative liquid reagent surplus x and a solid-phase matrix material require can be also x/a=n(integer) or x/a=n.m(non-integer), (n>=0, m>=0), computing element draws and adds after n solid-phase matrix material, then will in cavity, be full of the judgement of liquid reagent.
In other specific embodiments, computing element 113 can also relatively judge whether syringe pump 120 stops adding liquid reagent to solid-phase matrix material 175 in the following manner, when comparative result is na<=x< (n+1) a, whether the liquid reagent surplus x that is syringe pump 120 (comprises x=na at na with (n+1) between a, do not comprise x=(n+1) a), x is in the words in this interval, judgment result is that and add after n solid-phase matrix material 175, to in cavity 120, be full of liquid reagent, by this result feedback to automatic liquid feeding body equipment 108.And comparative result is x=(n+1) during a, judgment result is that and add after n+1 solid-phase matrix material, then will in cavity 120, be full of liquid reagent, by this result feedback to automatic liquid feeding body equipment 110.
In some embodiment, solid-phase matrix material 175 is for being exclusively used in the test strips of immunoassay.In other embodiment, a plurality of reagent receives bar and is positioned in an agent plate, in an agent plate, a plurality of reagent strips, consists of.And the liquid reagent being added is gold mark solution.
The present invention also comprises the equipment 110 of automatic liquid feeding body, and as shown in Figure 2, this equipment is compared with traditional equipment 108, has set up a computing element 113, by this computing element 113, uses method of the present invention to realize the function of full-automatic interpolation liquid reagent.Specifically, as shown in Figure 2, the equipment 110 of this automatic liquid feeding body comprises the cavity of receiving fluids reagent, it is syringe pump 120, and the driver 142 that drives syringe pump work, controller 114 and liquid is added to the shower nozzle 128 of the external world's (on solid-phase matrix material), also comprises computing element 113 certainly.The piston 118 that wherein syringe pump 120 comprises shell 162 and is positioned at syringe pump, this piston 118 is connected with driver 142, the other end of driver 142 is connected with controller 114 simultaneously, controller 114 is connected with computing element 113 in addition simultaneously, and in computing element, all results are all passed in controller.Controller 114 is also connected with shower nozzle 128, to control the liquid adding procedure of shower nozzle.The other end at syringe pump 120 is also connected with a valve 145, and one side of valve leads to fluid reservoir 116 by feed pathway 150, for by the liquid reagent of fluid reservoir 116 130, such as gold mark solution is sent in syringe pump 120; The other end of valve 145 is connected to shower nozzle 128 by liquid outlet channel 152, for the liquid reagent of syringe pump 120 130 is sent to shower nozzle 128.Valve 145 control syringe pumps liquid reagent 130 turnover and guarantee feed liquor and fluid can not carried out simultaneously.In order to control the quantitative interpolation of liquid reagent, shower nozzle 128 comprises and is positioned at the nozzle 205 of shower nozzle bottom and the valve 204 of Control Nozzle folding.Before equipment starts operation, the amount of reagent a value of the solid-phase matrix material require that this need to be added is preset in computing element 113, when the equipment 110 of this automatic liquid feeding body comes into operation, under the jointly controlling of controller 114 and computing element 113, by driver 142, drive, piston 118 withdraws, while valve 145 leads to feed pathway 150 ends to be opened, the piston 118 of syringe pump 120 will be full of liquid reagent 130 by motion in syringe pump 120, then computing element 113 the method according to this invention are carried out computing and comparison, and send operation result and judged result to controller 114, by controller 114, according to instruction, order about syringe pump 120 and carry out next step operation.Specifically, after computing element 113 calculates the liquid reagent surplus x in syringe pump 120, computing element 113 compares x value with the amount of liquid a that the host material being preset in computing element 113 needs, when comparative result is that x value is while being more than or equal to a value, computing element 113 is judged and can be continued to add liquid reagent to next host material, this result is transported in controller 114, by controller 114, controlled, that is: valve 145 is opened towards liquid outlet channel 152 ends, the piston 118 of syringe pump is pushed ahead under the driving of driver 142, make the liquid reagent in syringe pump 120 arrive shower nozzle by liquid outlet channel 152, shower nozzle is under the effect of controller 114 simultaneously, the interior valve of establishing 204 is opened, liquid reagent 130 is ejected on the solid-phase matrix material 175 that is arranged in assigned address by nozzle 205.Identical with traditional interpolation equipment, the moving line of nozzle as shown in Figure 6 and Figure 7, nozzle is according to the first order to the 5th circuit, by the path of A-B-C-D-E-F-G-H-I-J, moved, and solid-phase matrix material 175 is by continuous also continual 5 moving lines 401,402 that are transported to nozzle of conveyer belt, 403, on 404,405, allow nozzle 205 carry out liquid interpolation.Meanwhile, after each host material has been added, a route has moved, while arriving the terminal B of the first circuit 401 such as nozzle 205, controller 114 is by this information conveyance to computing element 113, and computing element 113 calculates liquid reagent surplus x again, and comparison x and a, if during x>=a, continue operation above, nozzle moves to C end by B end to start to add.So move in circles, until computing element 113 is reached a conclusion while being x<a, be when in syringe pump 120, liquid reagent surplus x is less than the requirement a of a solid-phase matrix material, such as, now nozzle 205 has arrived the terminal H of the 4th route, computing element 113 draws and stops adding liquid to the result on solid-phase matrix material, by this result feedback to controller 114, controller 114 makes nozzle stop adding the action of liquid, with Time Controller 114, make syringe pump 120 stop fluid according to instruction, valve 145 is closed towards liquid outlet channel end simultaneously, and valve 145 is opened towards feed pathway end, by feed pathway 150, the liquid reagent of fluid reservoir 116 is drawn in syringe pump 120 and is full of syringe pump 120.Then, under the control of computing element 113 and controller 114, nozzle 205 moves to I end from H point, then starts the interpolation of the 5th circuit 405, realizes automatic interpolation next time.Circulation, realizes the automation mechanized operation of this equipment and so forth.
And the comparison formula arranging in computing element 113 is x/a=n, or during x/a=n.m, controller 114 Control Nozzles 205 have added after n host material, such as nozzle 205 has now arrived behind the terminal J place of the 5th circuit, nozzle stops adding liquid and turns back to initiating terminal A point, meanwhile, controller 114 is opened valve 145 connection feed pathway places, makes the liquid reagent of fluid reservoir 116 enter into syringe pump 120 and be full of syringe pump.Then, controller 114 makes valve 145 cut out feed pathway end again, and opens liquid outlet channel end, makes liquid reagent arrive shower nozzle 128, makes nozzle 205 start the interpolation of next round.
Certainly, computing element 113 in this equipment 110 automatically adding can also be set another kind of account form, be about to liquid reagent surplus x and default a value comparison, draw x na and (n+1) a(comprise x=na) between, thereby judge, add after n solid-phase matrix material, equipment 110 stops adding, and liquid is full of to syringe pump 120, then carries out operation next time.Or, as x=(n+1) during a, to judge and add after n+1 solid-phase matrix material, equipment 110 stops interpolation, and liquid is full of to syringe pump, then carries out operation next time.
Claims (27)
1. a method for automatic liquid feeding body reagent, comprises the following steps:
Liquid reagent automatic powder adding is added at least one solid-phase matrix material;
Wherein, liquid reagent is arranged in a cavity;
Calculate the liquid reagent surplus x in this cavity;
Allow the amount of reagent a of liquid reagent surplus x and a solid-phase matrix material require compare, draw comparative result;
According to comparative result, judge whether to continue to add liquid reagent to solid-phase matrix material.
2. method according to claim 1, is characterized in that, when liquid reagent surplus x is more than or equal to the amount of reagent a of a solid-phase matrix material require, liquid reagent is continued to add on next solid-phase matrix material.
3. method according to claim 1, is characterized in that, when liquid reagent surplus x is less than the amount of reagent a of a solid-phase matrix material require, stops adding liquid reagent to next solid-phase matrix material.
4. method according to claim 3, is characterized in that, allows liquid reagent be full of described cavity.
5. method according to claim 1, it is characterized in that, when the comparative result of x and a is x/a=n (integer), or x/a=n.m(non-integer), continue to add liquid reagent and stop adding after n solid-phase matrix material, then allow liquid reagent be full of described cavity.
6. method according to claim 1, it is characterized in that, when the comparative result of x and a is na<=x< (n+1) a, wherein n is integer, n>=0, continue to add liquid reagent and stop adding after n solid-phase matrix material, then allow liquid reagent be full of described cavity.
7. method according to claim 1, is characterized in that, when the comparative result of x and a is x=(n+1) a, wherein n is integer, n>=0, continues to add liquid reagent and stops adding after n+1 solid-phase matrix material, then allow liquid reagent be full of described cavity.
8. according to the method one of claim 1-7 Suo Shu, it is characterized in that, by a computing element, compare x and a, and draw comparative result.
9. method according to claim 8, is characterized in that, computing element judges whether to continue to add liquid reagent to solid-phase matrix material by comparative result.
10. method according to claim 8, is characterized in that, by computing element, calculates liquid reagent surplus x in cavity, x=cavity total capacity y-na, and wherein, n is solid-phase matrix material quantity, n>=0.
11. methods according to claim 10, is characterized in that, the liquid reagent amount z that liquid reagent surplus x=cavity total capacity y-has added.
12. methods according to claim 1, is characterized in that, the test strips that described solid-phase matrix material is immunoassay.
13. methods according to claim 1, is characterized in that, described liquid reagent is the gold mark solution for immunodiagnosis.
14. an equipment for automatic liquid feeding body reagent, comprises a cavity, for receiving fluids reagent; Wherein, this equipment also comprises computing element, by the liquid reagent surplus x in this computing element comparison cavity and the liquid reagent requirement a of a solid-phase matrix material, and draws comparative result.
15. equipment according to claim 14, is characterized in that, this computing element judges whether to continue to add liquid reagent to solid-phase matrix material by comparative result.
16. equipment according to claim 15, is characterized in that, computing element feeds back to this equipment by judged result.
17. equipment according to claim 15, is characterized in that, when the comparative result that computing element obtains is x>=a, this equipment continues to add liquid reagent to next solid-phase matrix material.
18. equipment according to claim 15, is characterized in that, when the comparative result that computing element obtains is x<a, this equipment stops adding liquid reagent to next solid-phase matrix material; And this equipment is full of cavity by liquid reagent.
19. equipment according to claim 15, it is characterized in that, the comparative result that computing element obtains is x/a=n (integer), or x/a=n.m(non-integer), this equipment continues to add liquid reagent and stops adding after n solid-phase matrix material, then liquid reagent is full of to cavity.
20. equipment according to claim 15, it is characterized in that, when the comparative result that computing element obtains is na<=x< (n+1) a, wherein n is integer, n>=0, this equipment continues to add liquid reagent and stops adding after n solid-phase matrix material, then liquid reagent is full of to cavity.
21. equipment according to claim 15, it is characterized in that, when the comparative result that computing element obtains is x=(n+1) a, wherein n is integer, n>=0, this equipment continues to add liquid reagent and stops adding after n+1 solid-phase matrix material, then liquid reagent is full of to cavity.
22. according to the equipment one of claim 14-21 Suo Shu, it is characterized in that, computing element calculates the liquid reagent surplus x in cavity.
23. equipment according to claim 22, is characterized in that, x=cavity total capacity y-na; Wherein, n is solid-phase matrix material quantity, n>=0.
24. equipment according to claim 22, is characterized in that, the liquid reagent amount z that x=cavity total capacity y-has added.
25. equipment according to claim 14, is characterized in that, this equipment also comprises the shower nozzle being connected with cavity one end, the driver and the controller that are connected with the cavity other end.
26. equipment according to claim 25, is characterized in that, described computing element is connected with controller.
27. equipment according to claim 26, is characterized in that, this equipment also comprises a fluid reservoir, for storing liquid reagent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210317128.1A CN103657548A (en) | 2012-08-31 | 2012-08-31 | Method and equipment for automatically adding liquid reagent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210317128.1A CN103657548A (en) | 2012-08-31 | 2012-08-31 | Method and equipment for automatically adding liquid reagent |
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| Publication Number | Publication Date |
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| CN103657548A true CN103657548A (en) | 2014-03-26 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201210317128.1A Pending CN103657548A (en) | 2012-08-31 | 2012-08-31 | Method and equipment for automatically adding liquid reagent |
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| JPH05196626A (en) * | 1991-06-26 | 1993-08-06 | Boehringer Mannheim Gmbh | Analyzer for automatic analysis of body fluid |
| US6447729B1 (en) * | 1993-02-01 | 2002-09-10 | Labsystems Oy | Method and means for magnetic particle specific binding assay |
| CN1315913A (en) * | 1998-07-07 | 2001-10-03 | 笛卡尔技术公司 | Tip design and random access array for microfluidic transfer |
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Application publication date: 20140326 |