CN103651268B - A kind of cultural method of Portunus pelagicus wheel animalcule bait - Google Patents
A kind of cultural method of Portunus pelagicus wheel animalcule bait Download PDFInfo
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- CN103651268B CN103651268B CN201310646536.6A CN201310646536A CN103651268B CN 103651268 B CN103651268 B CN 103651268B CN 201310646536 A CN201310646536 A CN 201310646536A CN 103651268 B CN103651268 B CN 103651268B
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Abstract
The invention discloses a kind of cultural method of Portunus pelagicus wheel animalcule bait, comprise the following steps: throwing in advance in (1) culture pond has Micro Algae, inoculating rotifer in culture pond, and the inoculum density of wheel animalcule is 100 ~ 200/mL; (2), in incubation, yeast of throwing something and feeding in culture pond every day, the feeding volume of every day is every 10
6individual wheel animalcule is thrown something and fed yeast 0.8 ~ 1.2g, cultivates and gathers in the crops wheel animalcule after 8 ~ 10 days, Micro Algae of throwing something and feeding in culture pond again before results.In culture pond, throw in the bait of Micro Algae as wheel animalcule in advance, but wheel animalcule will faster than the reproduction speed of Micro Algae to the consumption of Micro Algae, therefore, yeast of also will throwing something and feeding in the incubation of wheel animalcule; Because wheel animalcule is filter-feeding, can not be too low in the amount of yeast of throwing something and feeding, too low, can cause can not being taken in by wheel animalcule; In addition, Micro Algae of throwing something and feeding again before results, lack highly unsaturated fatty acid with the wheel animalcule of Yeast Cultivation, Micro Algae can balance the nutrition of wheel animalcule, thus more meets the growth needs of Portunus pelagicus.
Description
Technical field
The present invention relates to cultivating portunus pelagicus technology, particularly relate to a kind of cultural method of Portunus pelagicus wheel animalcule bait.
Background technology
China's sea crab nursery mainly uses wheel animalcule and artemia nauplii two kinds of living baits.Wheel animalcule can large-scale culture, but technology is unstable, often occurs that wheel animalcule is under-supply in nursery; Artemia nauplii lack highly unsaturated fatty acid, its fortification is current to solve not yet completely, and the isolation technics of the dead ovum of halogen worm wait improve perfect.Wheel animalcule is the excellent bait of zoea, but mass propgation also has because of difficulty.Therefore, the wheel animalcule culture technique studying a kind of high and stable yields is needed.
Summary of the invention
For above-mentioned technical problem, the invention provides a kind of cultural method of Portunus pelagicus wheel animalcule bait of stable, high yield.
Technical scheme provided by the invention is:
A cultural method for Portunus pelagicus wheel animalcule bait, comprises the following steps:
(1) in culture pond, input has Micro Algae, inoculating rotifer in culture pond in advance, and the inoculum density of wheel animalcule is 100 ~ 200/mL;
(2), in incubation, yeast of throwing something and feeding in culture pond every day, the feeding volume of every day is every 10
6individual wheel animalcule is thrown something and fed yeast 0.8 ~ 1.2g, cultivates and gathers in the crops wheel animalcule after 8 ~ 10 days, Micro Algae of throwing something and feeding in culture pond again before results.
Preferably, in the cultural method of described Portunus pelagicus wheel animalcule bait, in described step (2), in incubation, also inflate in culture pond.
Preferably, in the cultural method of described Portunus pelagicus wheel animalcule bait, in described step (2), when the density of wheel animalcule is less than 500/mL, every natural gift are thrown something and fed for 1 ~ 2 time yeast in culture pond, when the density of wheel animalcule is greater than 500/mL, every natural gift are thrown something and fed for 2 ~ 5 times yeast in culture pond.
Preferably, in the cultural method of described Portunus pelagicus wheel animalcule bait, in step (1), in culture pond, cultivating Micro Algae, treating that Micro Algae breeding is to making the water body in culture pond show color, then to culture pond inoculating rotifer.
Preferably, in the cultural method of described Portunus pelagicus wheel animalcule bait, in described step (1), cultivate Micro Algae in culture pond before, in culture pond, add cultivation salt, make the concentration of each cultivation salt in the water body of culture pond be: sodium nitrate 60ppm, potassium dihydrogen phosphate 5ppm, ironic citrate 1ppm, accesses to Unicellular Algae in culture pond, and the volume ratio of the water body of access amount and culture pond is 1: 2 ~ 5.
Preferably, in the cultural method of described Portunus pelagicus wheel animalcule bait, in described step (1), depth direction along described culture pond in described culture pond is placed with multilayer gas tube, every layer of gas tube is included in the multiple gas tubes extended in same level, the bottom of the tube wall of each gas tube has some pores, and multilayer gas tube is connected to air pump by a flexible pipe, and the link position of every layer of gas tube and flexible pipe is provided with valve; In described step (2), in incubation, multilayer gas tube opens inflation from the bottom to top successively, the inflationtime of every layer of gas tube is 2 ~ 3 minutes, continuous circulation 4 ~ 5 times, thus complete a gas replenishment process, once inflated at interval of 1 ~ 2 hour.
Preferably, in the cultural method of described Portunus pelagicus wheel animalcule bait, in described step (1), each gas tube is also connected with lamellar parts, multilayer gas tube is fixed on a movable support, described movable support is can the relatively described fixed support mode that swings 5 ~ 10 ° be arranged on a fixed support, and described movable support is also connected to motor; In described step (2), when inflating, described motor also drives described movable support at every turn, thus makes the water body in described lamellar parts disturbance culture pond.
Preferably, in the cultural method of described Portunus pelagicus wheel animalcule bait, the lamellar parts on each gas tube are arrange with the radial skew 5 ~ 10 ° of this gas tube.
The cultural method of wheel animalcule bait of the present invention has following beneficial effect: in culture pond, throw in the bait of Micro Algae as wheel animalcule in advance, but wheel animalcule will faster than the reproduction speed of Micro Algae to the consumption of Micro Algae, therefore, also to throw something and feed in the incubation of wheel animalcule yeast; Because wheel animalcule is filter-feeding, can not be too low in the amount of yeast of throwing something and feeding, too low, can cause can not being taken in by wheel animalcule; In addition, Micro Algae of throwing something and feeding again before results, lack highly unsaturated fatty acid with the wheel animalcule of Yeast Cultivation, Micro Algae can balance the nutrition of wheel animalcule, thus more meets the growth needs of Portunus pelagicus.
Accompanying drawing explanation
Fig. 1 is the schematic flow sheet of the cultural method of Portunus pelagicus wheel animalcule bait of the present invention;
Fig. 2 is the structural representation of culture pond of the present invention;
Fig. 3 is the structural representation of gas tube of the present invention.
Embodiment
Below in conjunction with accompanying drawing, the present invention is described in further detail, can implement according to this with reference to specification word to make those skilled in the art.
As shown in Figure 1, the invention provides a kind of cultural method of Portunus pelagicus wheel animalcule bait, comprise the following steps: throwing in advance in (1) culture pond has Micro Algae, inoculating rotifer in culture pond, and the inoculum density of wheel animalcule is 100 ~ 200/mL (step 101); (2), in incubation, yeast of throwing something and feeding in culture pond every day, the feeding volume of every day is every 10
6individual wheel animalcule is thrown something and fed yeast 0.8 ~ 1.2g, cultivates and gathers in the crops wheel animalcule after 8 ~ 10 days, Micro Algae (step 102) of throwing something and feeding in culture pond again before results.
In above-mentioned steps (2), when Micro Algae can not meet the nutritional need of wheel animalcule, must based on yeast of throwing something and feeding, with 250 order silk cover filterings before throwing something and feeding.Use two days later with Micro Algae strengthening before results.The special mesh bag that available 200 mesh sieve thin,tough silk are made filters results.
Preferably, in described step (2), in incubation, also inflate in culture pond.
Preferably, in described step (2), when the density of wheel animalcule is less than 500/mL, every natural gift are thrown something and fed for 1 ~ 2 time yeast in culture pond, and when the density of wheel animalcule is greater than 500/mL, every natural gift are thrown something and fed for 2 ~ 5 times yeast in culture pond.Namely, when early stage wheel animalcule density is low, bait can suitably be thrown more, and when wheel animalcule density is higher, bait can suitably be thrown less.
Preferably, in step (1), in culture pond, cultivating Micro Algae, treating that Micro Algae breeding is to making the water body in culture pond show color, then to culture pond inoculating rotifer.Specifically, in described step (1), cultivate Micro Algae in culture pond before, cultivation salt is added in culture pond, the concentration of each cultivation salt in the water body of culture pond is made to be: sodium nitrate 60ppm, potassium dihydrogen phosphate 5ppm, ironic citrate 1ppm, accessed to by Unicellular Algae in culture pond, the volume ratio of the water body of access amount and culture pond is 1: 2 ~ 5.Inoculation time is typically chosen between 8:00 ~ 10:00 in the morning and carries out.Micro Algae is at normal temperatures through the cultivation of 10 days, and concentration reaches 4 × 10
5individual/more than mL, now can be used as bait feeding.
Wheel animalcule culture pond, through the cultivation of 7 days, just must carry out down pond, with remove residual bait corruption cause be unfavorable for the factor that wheel animalcule is cultivated.All the more so when High Density Cultivation.In order to ensure that High Density Cultivation wheel animalcule can normally carry out, preferably ensure to fall for 2 ~ 3 days 1 pond.Filter wheel animalcule like this and a part of wheel animalcule may be lost in transfer process, but the object reaching short time High Density Cultivation can be ensured.
In a preferred embodiment, in described step (1), as shown in Figure 1, depth direction along described culture pond in described culture pond is placed with multilayer gas tube, every layer of gas tube is included in the multiple gas tubes extended in same level, the bottom of the tube wall of each gas tube has some pores, and multilayer gas tube is connected to air pump by a flexible pipe, and the link position of every layer of gas tube and flexible pipe is provided with valve; In described step (2), in incubation, multilayer gas tube opens inflation from the bottom to top successively, the inflationtime of every layer of gas tube is 2 ~ 3 minutes, continuous circulation 4 ~ 5 times, thus complete a gas replenishment process, once inflated at interval of 1 ~ 2 hour.Multiple gas tubes included by every layer of gas tube all be arranged in parallel, and the gas tube between adjacent two layers gas tube is then perpendicular to one another.
In once inflating, continuous circulation 4 ~ 5 times, in each circulation, multilayer gas tube opens inflation from the bottom to top successively, the dissolved oxygen concentration in water body can be made on the one hand as far as possible even, meet the besoin de respirer of wheel animalcule in whole water body; On the other hand, in wheel animalcule reproductive process, residual bait and ight soil get more and more, and the ammonia nitrogen amount in water also increases thereupon, will suppress the growth of wheel animalcule, and dissolved oxygen reduces the ammonia nitrogen amount in water body by contributing to, and above-mentioned design also contributes to preventing the ammonia nitrogen amount of local too high; Another aspect, the airing form of this circulation, also can play the effect of stirring, makes the various nutrient component in the waters near each layer gas tube and wheel animalcule all be in comparatively slight flow regime, thus more contributes to the growth of wheel animalcule.
Further, in described step (1), each gas tube is also connected with lamellar parts, multilayer gas tube is fixed on a movable support, described movable support is can the relatively described fixed support mode that swings 5 ~ 10 ° be arranged on a fixed support, and described movable support is also connected to motor; In described step (2), when inflating, described motor also drives described movable support at every turn, thus makes the water body in described lamellar parts disturbance culture pond.
When multilayer gas tube is all fixed on a movable support, when driving the relative fixed support of this movable support to swing a low-angle by motor, lamellar parts meeting disturbance water body, larger stirring action is produced to water body, thus makes dissolved oxygen more even, and break the inactive state of water body, improve the environment in water body, thus making the growing state of wheel animalcule better, the survival rate of wheel animalcule is the highest, is best suited for the cultivation of high density wheel animalcule.Movable support pendulum angle is excessive, excessive to the disturbance of water body, is also unfavorable for the survival and growth of wheel animalcule.
When the lamellar parts on each gas tube are arrange with the radial skew 5 ~ 10 ° of this gas tube, when multilayer gas tube moves, the most suitable to the level of disruption of water body.
Although embodiment of the present invention are open as above, but it is not restricted to listed in specification and embodiment utilization, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can easily realize other amendment, therefore do not deviating under the universal that claim and equivalency range limit, the present invention is not limited to specific details and illustrates here and the legend described.
Claims (5)
1. a cultural method for Portunus pelagicus wheel animalcule bait, is characterized in that, comprises the following steps:
(1) in culture pond, input has Micro Algae, inoculating rotifer in culture pond in advance, and the inoculum density of wheel animalcule is 100 ~ 200/mL;
In described step (1), depth direction along described culture pond in described culture pond is placed with multilayer gas tube, every layer of gas tube is included in the multiple gas tubes extended in same level, the bottom of the tube wall of each gas tube has some pores, multilayer gas tube is connected to air pump by a flexible pipe, and the link position of every layer of gas tube and flexible pipe is provided with valve; Each gas tube is also connected with lamellar parts, and multilayer gas tube is fixed on a movable support, and described movable support is can relatively swinging the mode of 5 ~ 10 ° and be arranged on a fixed support by fixed support, and described movable support is also connected to motor;
(2), in incubation, yeast of throwing something and feeding in culture pond every day, the feeding volume of every day is every 10
6individual wheel animalcule is thrown something and fed yeast 0.8 ~ 1.2g, cultivates and gathers in the crops wheel animalcule after 8 ~ 10 days, Micro Algae of throwing something and feeding in culture pond again before results;
In described step (2), in incubation, multilayer gas tube opens inflation from the bottom to top successively, the inflationtime of every layer of gas tube is 2 ~ 3 minutes, continuous circulation 4 ~ 5 times, thus complete a gas replenishment process, once inflated at interval of 1 ~ 2 hour; During each inflation, described motor also drives described movable support, thus makes the water body in described lamellar parts disturbance culture pond.
2. the cultural method of Portunus pelagicus wheel animalcule bait as claimed in claim 1, it is characterized in that, in described step (2), when the density of wheel animalcule is less than 500/mL, every natural gift are thrown something and fed for 1 ~ 2 time yeast in culture pond, when the density of wheel animalcule is greater than 500/mL, every natural gift are thrown something and fed for 2 ~ 5 times yeast in culture pond.
3. the cultural method of Portunus pelagicus wheel animalcule bait as claimed in claim 1, it is characterized in that, in step (1), in culture pond, cultivate Micro Algae, treat that Micro Algae breeding is to making the water body in culture pond show color, then to culture pond inoculating rotifer.
4. the cultural method of Portunus pelagicus wheel animalcule bait as claimed in claim 3, it is characterized in that, in described step (1), cultivate Micro Algae in culture pond before, in culture pond, add cultivation salt, make the concentration of each cultivation salt in the water body of culture pond be: sodium nitrate 60ppm, potassium dihydrogen phosphate 5ppm, ironic citrate 1ppm, accesses to Unicellular Algae in culture pond, and the volume ratio of the water body of access amount and culture pond is 1: 2 ~ 5.
5. the cultural method of Portunus pelagicus wheel animalcule bait as claimed in claim 1, it is characterized in that, the lamellar parts on each gas tube are arrange with the radial skew 5 ~ 10 ° of this gas tube.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105475238A (en) * | 2015-12-05 | 2016-04-13 | 陕西省水产研究所 | Non-circulating-water cultivation method of rotifera |
| CN105393991B (en) * | 2015-12-09 | 2018-08-21 | 苏州大学 | A kind of detection method of toxicity of ammonia nitrogen to Brachionus calyciflorus |
| CN108124796A (en) * | 2018-01-08 | 2018-06-08 | 广西壮族自治区海洋研究所 | A kind of method for cultivating wheel animalcule |
| CN108719151A (en) * | 2018-05-15 | 2018-11-02 | 盘锦光合蟹业有限公司 | A kind of circulating outdoor earth pond wheel animalcule culture apparatus and method |
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| CN201032792Y (en) * | 2007-04-20 | 2008-03-12 | 宁波市海洋与渔业研究院 | Bottom oxygen replenishing device for cultivating portunid |
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| CN201451060U (en) * | 2009-06-16 | 2010-05-12 | 陈杰 | Tridimensional micropore aeration and oxygenation device |
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