CN103650697A - Method for promoting cynomorium songaricum seeds to germinate - Google Patents

Abstract

The invention provides a method for promoting cynomorium songaricum seeds to germinate. The method comprises the following steps: collecting the cynomorium songaricum seeds, treating nitraria tangutorum bobr roots, preparing plaster, disinfecting the cynomorium songaricum seeds, planting the cynomorium songaricum seeds and dripping a nitraria tangutorum bobr root extracting solution. The cynomorium songaricum seeds have great possibility of being contacted with hosts, namely the nitraria tangutorum bobr roots, so as to establish a parasitic relation and promote a condition of germinating the cynomorium songaricum seeds; the germination period of the cynomorium songaricum seeds is shortened and the germination rate is greatly improved; the germination rate is improved to 45.5% from existing 10%; according to the method for promoting the cynomorium songaricum seeds to germinate, the cynomorium songaricum seeds are convenient to store and transport; artificial inoculation is convenient to carry out, the operation is simple and the cost is low; large-area popularization is easy to realize.

Description

A kind of method that promotes that cynomorium songaricum seed is sprouted

Technical field

The present invention relates to a kind of method that promotes that cynomorium songaricum seed is sprouted.

 

Background technology

Cynomorium songaricum (Cynomorium songaricum Rupr.) is Cynomoriaceae cynomorium songaricum platymiscium, multiparasitism is on the root of the white thorn of zygophyllaceae (Zygophy llaceae) plant, on the harmel root that parasitizes this section having, belong to obligate root parasitism plant, its fleshy stem is the traditional Chinese medicine material of China's Desert Area preciousness.Research in recent years shows, cynomorium songaricum is in anti-cancer, anticancer, treatment stomach ulcer, indigestion, impotence and premature ejaculation, the weak deficiency of Yin of gas, stomachache, hyperhydrochloria, kidney tonifying ease constipation, waist knee flaccidity (Chinese medicine voluminous dictionary), immunological regulation, delay senility, the aspect such as prevention and cure of cardiovascular disease, the minimizing for the treatment of leucocyte has important medical value; In anaesthetic, be also used for antidiarrheal stomach invigorating, the illnesss such as treatment intestines heat, gastritis, indigestion, dysentery.Therefore cynomorium songaricum is the resources of medicinal plant that arid-desert area produces, and has important value of exploiting and utilizing.

Cynomorium songaricum is prennical root obligatory parasitism draft, and seed is subsphaeroidal, diameter 1mm, and kind skin is hard.Mature seed is arranged in nutlet, and pericarp is hard, and all lignification, is brown.Embryo is spherical, and cytoplasm is dense and core is large, there is no the differentiation of plumule and plumular axis, and prior art discloses: cynomorium songaricum seed be difficult to sprout or germination rate low, be called as " dormancy " of seed.The main cause that causes cynomorium songaricum seed dormancy is the existence of Germination suppression material in the hard pericarp of cynomorium songaricum nutlet and seed.Chinese patent discloses " a kind of method of inducement of cynomorium songaricum seed callus and special culture media thereof ".The medium of this inducement of cynomorium songaricum seed callus is in B5 medium, to add the medium that following additive obtains; Its additive comprise 2,4-dichlorphenoxyacetic acid (2,4-D), kinetin (KT) and gibberellin (GA3).In order to improve the induction efficiency of callus, additive also can comprise tyrosine, and its final concentration is below 2gL-1.Application the method cynomorium songaricum seed germination rate is on the low side, only have 10%-20%, and cost is relatively high, and complicated operation, is not easy to spread.

Summary of the invention

By observation and test for many years, the object of the present invention is to provide a kind of method that promotes that cynomorium songaricum seed is sprouted.The method is used exogenous hormone GA ₃stimulate the method combining to improve the germination rate of cynomorium songaricum seed with host's root extract, germination rate can be up to 45.5%.

The object of the invention is to be achieved through the following technical solutions:

Promote the method that cynomorium songaricum seed is sprouted, the steps include:

A. at the bottom of annual August, gather cynomorium songaricum seed, gather cynomorium songaricum seed normal temperature laboratory storage four months;

B. get the Bai Cigen phloem of natural air drying and put into acetone soln immersion 24h, then diluted for use;

C. producing of clay: with running water, rinse the sandy soil of taking from cynomorium songaricum growth habitat, after staticly settling, remove unnecessary water, make clay, then in clay, add disinfecting solution of potassium permanganate, standby;

D. choose the mercuric chloride sterilization 10 minutes for seed of a step, then soak with liquor natrii hypochloritis, and magnetic agitation 4h,

E. the cotton seed paper of selecting, on cotton seed paper surface, with brush, evenly brush the clay that c step is made, cynomorium songaricum seed after the sterilization of d step is contained in plastic bottle, on bottle cap, stamp the aperture that diameter is 2-3mm, then on clay, spray cynomorium songaricum seed, with skim toilet paper, cover cynomorium songaricum seed surface, by Gibberellins solution, soak 12h;

F. will e step soak the cynomorium songaricum seed that is furnished with peeling in cotton seed paper and take out, be placed in baking oven and sterilize after 4-6h, be seated in culture dish, with buret, drip Bai Cigen extract, observe cynomorium songaricum seed and sprout.

The beneficial effect of advantage of the present invention and generation is:

1, the present invention gently rubs cynomorium songaricum seed with the hands with sand paper, abolishes the hard pericarp of cynomorium songaricum, breaks seed dormancy, shortens the germinating cycle of seed, increases substantially the germination rate of cynomorium songaricum seed, and germination rate reaches more than 40%;

2, Bai Cigen soaks through acetone soln, make parasite, the cynomorium songaricum seed of peeling is placed in cotton seed paper, drip Bai Cigen extract, be seeded in the scope of white thorn root, can greatly increase the touch opportunity of cynomorium songaricum seed and Bai Cigen, for cynomorium songaricum seed is parasitic and growth provides needed material.

3, selecting the effect of cotton seed paper is that after inoculating, seed paper can absorb water and water conservation, when soil property is dry, and needed moisture in the time of can guaranteeing cynomorium songaricum seed sprouting.

4, the present invention facilitates storage and the transportation of cynomorium songaricum seed, facilitates artificial infection, simple to operate, with low cost, is easy to spread.

Accompanying drawing explanation

Fig. 1 is that cotton seed paper of the present invention is coated the clay that 1-2mm is thick, evenly sprays 300 cynomorium songaricum seed inoculating paper front elevations;

Fig. 2 is that Fig. 1 is as the inoculating paper back view of bottom;

Fig. 3 is that Fig. 1 covers one deck toilet paper in the whole inoculating paper schematic diagram on cynomorium songaricum seed surface.

Embodiment

Below in conjunction with drawings and Examples, then person's character is understood to technical scheme is further described:

Promote the method that cynomorium songaricum seed is sprouted, comprise the following steps:

1, seed storage: gather at the bottom of in August, 2010, seed is fallen in manual stranding from cynomorium songaricum plant, and natural wind is raised, and clean being contained in kraft envelope bag is placed on normal temperature laboratory storage later, and storage time is four months;

2, producing of Bai Cigen extract: choose robust growth, the phloem of fresh and alive Bai Cigen, air-dry under field conditions (factors), add 10ml acetone soln to soak 24h the every 100g of Bai Cigen phloem after natural air drying, then dilute 10 times standby;

3, producing of clay: with running water, rinse the sandy soil of taking from cynomorium songaricum growth habitat, after staticly settling, remove unnecessary water, make clay, then in clay, add the disinfecting solution of potassium permanganate of every 1kg clay 100ml10%, standby;

3, choosing the cynomorium songaricum seed full, particle is large sterilizes 10 minutes with 0.1% mercuric chloride, with running water, rinse and in 10% liquor natrii hypochloritis, soak 12h 10-15 time, then with running water, rinse 3-5 time, after having rinsed, be contained in and in beaker, use magnetic agitation 4h, in whipping process, if while there is muddiness in beaker, change immediately water.After stirring finishes, with running water, rinse 6-8 time, then use aseptic water washing 2 times, with softening kind of the skin of liquor natrii hypochloritis of mass concentration 10%, abolish hard pericarp, and magnetic agitation 4h;

4, select the cotton seed paper of 19*6cm, after the effect of selecting cotton seed paper is inoculation, seed paper can absorb water and water conservation, when soil property is dry, and needed moisture in the time of can guaranteeing cynomorium songaricum seed sprouting; Then on cotton seed paper surface, with brush, evenly brush clay, thickness is 1-2mm; Cynomorium songaricum seed after sterilization is contained in plastic bottle, on bottle cap, stamps the aperture that diameter is 2-3mm, then toward clay on, spray on every inoculating paper, every inoculating paper plant 300-320-cynomorium songaricum seed; Seed covers cynomorium songaricum seed surface with skim toilet paper after spraying evenly, and toilet paper is that toilet paper can not be too thick for fixing dropping of cynomorium songaricum seed, the too thick sprouting of can impact inoculating rear cynomorium songaricum seed;

5, then will be placed in cynomorium songaricum seed in clean cotton seed paper, cotton seed paper sucks the moisture of the surface of the seed fast, by the Gibberellins solution of 50mg/l, soaks 12h.Then be placed in 150 ℃ of baking ovens and sterilize after 4-6h, place in lower Ф 9cm culture dish, by the cotton seed paper after sterilization, adopt two cotton seed paper methods to cultivate.Cultivation temperature is that 25 ℃ of-35 ℃ of artificial intelligence incubators carry out alternating temperature and secretly cultivate, and culture dish is wrapped up in lighttight black paper bag.Every 24h observes once, dry culture dish is dripped to Bai Cigen extract 4-6 with buret at every turn and drips, and dripping Bai Cigen extract can not be too much, makes filter paper or the surface of the seed keep moisture state best.

Cynomorium songaricum seed starts to sprout after the cultivation of 100 days, continue to sprout 60, after cynomorium songaricum seed is sprouted, first radiculodium by embryo grows column sprout, and front end has a thick head, contains a large amount of starch graiies, after being that host contacts, adhesion is also invaded host's root cortex, draws nutrient and enlarges into cynomorium songaricum sprout, and then form new cynomorium songaricum plant at host's root.

Claims (1)

1. promote the method that cynomorium songaricum seed is sprouted, the steps include:

A. at the bottom of annual August, gather cynomorium songaricum seed, gather cynomorium songaricum seed normal temperature laboratory storage four months;

B. get the Bai Cigen phloem of natural air drying and put into acetone soln immersion 24h, then diluted for use;

C. producing of clay: with running water, rinse the sandy soil of taking from cynomorium songaricum growing environment, after staticly settling, remove unnecessary water, make clay, then in clay, add disinfecting solution of potassium permanganate, standby;

D. choose the mercuric chloride sterilization 10 minutes for cynomorium songaricum seed of a step, then soak with liquor natrii hypochloritis, and magnetic agitation 4h;

E. select cotton seed paper, on cotton seed paper surface, with brush, evenly brush the clay that c step is made, cynomorium songaricum seed after the sterilization of d step is contained in plastic bottle, on bottle cap, stamp the aperture that diameter is 2-3mm, then on clay, spray cynomorium songaricum seed, with a thin layer toilet paper, cover cynomorium songaricum seed surface, by Gibberellins solution, soak 12h;

F. will e step soak the cynomorium songaricum seed that is furnished with peeling in cotton seed paper and take out, be placed in baking oven and sterilize after 4-6h, be seated in culture dish, with buret, drip Bai Cigen extract, observe cynomorium songaricum seed and sprout.

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