CN103642938A - Biological detection method for black-streaked dwarf virus transmission rate of laodelphax striatellus - Google Patents
Biological detection method for black-streaked dwarf virus transmission rate of laodelphax striatellus Download PDFInfo
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- CN103642938A CN103642938A CN201310605649.1A CN201310605649A CN103642938A CN 103642938 A CN103642938 A CN 103642938A CN 201310605649 A CN201310605649 A CN 201310605649A CN 103642938 A CN103642938 A CN 103642938A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The invention relates to a living-body biological detection method, and specifically relates to a biological detection method for black-streaked dwarf virus transmission rate of laodelphax striatellus. The detection method comprises: breeding laodelphax striatellus on intensively cultivated black-streaked dwarf viral disease plants, after 7 days, transferring laodelphax striatellus forcedly bred with virus to healthy disease-free rice seedlings in the three-leaf-and-one-leaflet stage and enabling laodelphax striatellus to feed for 48 h, then transplanting eaten price seedlings to an observation garden, observing the occurrence time and the occurrence number of black-streaked dwarf viral disease, and obtaining the black-streaked dwarf virus transmission rate of laodelphax striatellus and the incubation period of the virus in the plant by statistics. The invention aims at providing an accurate, reliable, short-period scientific effective biological detection method for black-streaked dwarf virus transmission rate of laodelphax striatellus.
Description
Technical field
The present invention relates to a kind of living body biological and learn detection method, specifically refer to that a kind of small brown rice planthopper passes the detection method of malicious rate to black-streaked dwarf virus.
Background technology
Black streaked dwarf virus of rice is to cause a kind of virus disease that rice plant stunts, and this virus is mainly propagated by small brown rice planthopper.Small brown rice planthopper takes food after band poison on diseased plant, shifts while causing harm virus is reached to healthy tree.At present, small brown rice planthopper infects virus to interval of aobvious disease etc. to this viral spreading rate height, plant after with poison, there is no the detection method of complete practicality.
Summary of the invention
The object of the invention is to overcome above shortcomings in prior art, and provide accurately and reliably a kind of, the cycle is short, and scientific and effective small brown rice planthopper passes the biological detection method of malicious rate to black-streaked dwarf virus.
Realize technical scheme of the present invention as follows: a kind of small brown rice planthopper passes the biological detection method of malicious rate to black-streaked dwarf virus, mainly following steps, consist of:
(1) raise poison: gather black streak dwarf diseased plant, concentrate cultivation, collect on sufficient small brown rice planthopper access black streak dwarf diseased plant, keep the environment of black streak dwarf diseased plant and small brown rice planthopper normal growth, the small brown rice planthopper 6-8 days that feeds continuously;
(2) pass poison: the Glass tubing of selecting both ends open, Glass tubing mouth of pipe one end covers with gauze, the three leaf one heart stage rice seedlings that health is anosis are inserted in Glass tubing, then access the small brown rice planthopper of feeding through raising poison in step (1), every strain rice seedling connects a small brown rice planthopper, rice seedling has accessed the rear the other end with gauze cover upper glass pipe, keeps the normal growth of small brown rice planthopper and rice seedling, and rice seedling takes food 45-50 hour continuously;
(3) detect: get the rice seedling being taken food in step (2) and plant to observing garden, observe rice plant incidence in observation garden, until stable disease, record time, continuous days that morbidity occurs, statistics show that small brown rice planthopper is to the biography poison rate of black streak dwarf and viral latent period in plant body.
As preferably, the black streak dwarf diseased plant gathering in described step (1) is concentrated the Turnover Box of cultivation in insect-proof net chamber, each Turnover Box is planted 9 strain black streak dwarf diseased plants, and the fly net specification of insect-proof net chamber is 80 orders, and insect-proof net chamber length * wide * height is 50cm * 45cm * 70cm.
As preferably, the Glass tubing after described step (2) has accessed is vertically put into the Turnover Box that fills clear water, and rice seedling root is immersed in the water 1-2cm down.
As preferably, described observation garden is isolated from the outside with insect-proof net chamber, and uses the medicament of control small brown rice planthopper in observation garden, and the fly net specification of insect-proof net chamber is 80 orders.
As preferably, described Turnover Box length * wide * height is 50cm * 45cm * 30cm.
As preferably, described Glass tubing length is 15cm, and described diameter glass tube is 3.5cm.
The present invention has the following advantages and beneficial effect: the result that 1, statistics detects accurately and reliably, in whole testing process, all adopts fly net to be isolated from the outside, and gets rid of the extraneous poisonous insect source that passes and disturbs; 2, the cycle short, be chosen in three leaf one heart stage rice seedlings, rice plant easily shows the symptom of typically stunting after obtaining poison, has shortened the time that small brown rice planthopper passes malicious rate data that obtains, and shortens the test period, is more conducive to carrying out of this type of correlative study; 3, scientific and effective, provide a kind of small brown rice planthopper to pass malicious rate biological detection method accurately to black-streaked dwarf virus, solved the blank of current industry, for control small brown rice planthopper provides scientific basis to the propagation of black-streaked dwarf virus.
Accompanying drawing explanation
Fig. 1 is the schematic flow sheet of the embodiment of the present invention.
Embodiment
Below in conjunction with embodiment, the invention will be further described, but embodiments of the present invention are not limited to this.
Embodiment.
Referring to Fig. 1, small brown rice planthopper of the present invention passes the biological detection method of malicious rate to black-streaked dwarf virus, mainly following steps, consist of:
(1) raise poison: gather black streak dwarf diseased plant, concentrate cultivation in grow * wide * high Turnover Box for 50cm * 45cm * 30cm, each Turnover Box is planted 9 strains, Turnover Box is removed to insect-proof net chamber, the fly net specification of insect-proof net chamber is 80 orders, insect-proof net chamber length * wide * height is 50cm * 45cm * 70cm, and then on the diseased plant in field rice is collected sufficient small brown rice planthopper access fly net, the environment that keeps diseased plant and small brown rice planthopper normal growth, feed continuously 7 days, insect-proof net chamber can prevent moving into of peripheral small brown rice planthopper and the flight of netting interior small brown rice planthopper, and the space of migrating of the interior small brown rice planthopper of effective confinement cells, force small brown rice planthopper to stay on rice plant, improve the toxic effect rate of raising,
(2) pass poison: the Glass tubing of selecting both ends open, Glass tubing length is 15cm, diameter is 3.5cm, Glass tubing mouth of pipe one end covers with gauze, the three leaf one heart stage rice seedlings that health is anosis are inserted in Glass tubing, every Glass tubing one strain, then access the small brown rice planthopper of feeding through raising poison in step (1), every strain rice seedling connects a small brown rice planthopper, after rice seedling access worm with the other end of gauze cover upper glass pipe, Glass tubing is vertically put into the Turnover Box that fills clear water, guarantee that rice seedling root is immersed in the water down, 1-2cm is advisable, guarantee the normal growth of small brown rice planthopper and rice seedling, rice seedling takes food 48 hours continuously,
(3) detect: get the rice seedling being taken food in step (2) and plant to observing garden, observation garden is isolated from the outside with insect-proof net chamber, the fly net specification of insect-proof net chamber is 80 orders, getting rid of the extraneous poisonous insect source that passes disturbs, and use the medicament of control small brown rice planthopper in observation garden, avoiding small brown rice planthopper secondary to take food causes measurement result inaccurate, every day, rice plant incidence in observation garden observed in record, until stable disease, record time, continuous days that morbidity occurs, statistics show that small brown rice planthopper is to the biography poison rate of black streak dwarf and viral latent period in plant body.
The present invention all adopts insect-proof net chamber to be isolated from the outside in whole testing process, getting rid of the extraneous poisonous insect source that passes disturbs, while utilizing this invention technology for detection small brown rice planthopper to the malicious rate of the biography of black streak dwarf, being chosen in rice seedling (three leaf one heart stage) carries out, now detect, rice plant easily shows the symptom of typically stunting after obtaining poison, has shortened the time that small brown rice planthopper passes malicious rate data that obtains, shorten the test period, for control small brown rice planthopper provides scientific basis to the propagation of black-streaked dwarf virus.
Claims (6)
1. small brown rice planthopper passes a biological detection method for malicious rate to black-streaked dwarf virus, it is characterized in that: mainly following steps, consist of:
(1) raise poison: gather black streak dwarf diseased plant, concentrate cultivation, collect on sufficient small brown rice planthopper access black streak dwarf diseased plant, keep the environment of black streak dwarf diseased plant and small brown rice planthopper normal growth, the small brown rice planthopper 6-8 days that feeds continuously;
(2) pass poison: the Glass tubing of selecting both ends open, Glass tubing mouth of pipe one end covers with gauze, the three leaf one heart stage rice seedlings that health is anosis are inserted in Glass tubing, then access the small brown rice planthopper of feeding through raising poison in step (1), every strain rice seedling connects a small brown rice planthopper, rice seedling has accessed the rear the other end with gauze cover upper glass pipe, keeps the normal growth of small brown rice planthopper and rice seedling, and rice seedling takes food 45-50 hour continuously;
(3) detect: get the rice seedling being taken food in step (2) and plant to observing garden, observe rice plant incidence in observation garden, until stable disease, record time, continuous days that morbidity occurs, statistics show that small brown rice planthopper is to the biography poison rate of black streak dwarf and viral latent period in plant body.
2. small brown rice planthopper according to claim 1 passes the biological detection method of malicious rate to black-streaked dwarf virus, it is characterized in that: the black streak dwarf diseased plant gathering in described step (1) is concentrated the Turnover Box of cultivation in insect-proof net chamber, each Turnover Box is planted 9 strain black streak dwarf diseased plants, the fly net specification of insect-proof net chamber is 80 orders, and insect-proof net chamber length * wide * height is 50cm * 45cm * 70cm.
3. small brown rice planthopper according to claim 1 passes the biological detection method of malicious rate to black-streaked dwarf virus, it is characterized in that: the Glass tubing after described step (2) has accessed is vertically put into the Turnover Box that fills clear water, and rice seedling root is immersed in the water 1-2cm down.
4. small brown rice planthopper according to claim 1 passes the biological detection method of malicious rate to black-streaked dwarf virus, it is characterized in that: described observation garden is isolated from the outside with insect-proof net chamber, and use the medicament of control small brown rice planthopper in observation garden, the fly net specification of insect-proof net chamber is 80 orders.
5. according to the small brown rice planthopper described in claim 2 or 3, black-streaked dwarf virus is passed the biological detection method of malicious rate, it is characterized in that: described Turnover Box is long * wide * high is 50cm * 45cm * 30cm.
6. small brown rice planthopper according to claim 1 passes the biological detection method of malicious rate to black-streaked dwarf virus, it is characterized in that: described Glass tubing length is 15cm, and described diameter glass tube is 3.5cm.
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| CN201310605649.1A CN103642938A (en) | 2013-11-26 | 2013-11-26 | Biological detection method for black-streaked dwarf virus transmission rate of laodelphax striatellus |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104830998A (en) * | 2015-05-20 | 2015-08-12 | 宋立胜 | Molecular identification method for resistance of rice black streaked dwarf virus (RBSDV) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101633908A (en) * | 2009-09-01 | 2010-01-27 | 江苏省农业科学院 | Method for laodelphax striatellus to obtain rice black-streaked dwarf virus (RBSDV) from refrigerated diseased leaves |
| CN102533664A (en) * | 2012-01-09 | 2012-07-04 | 浙江大学 | Hybridoma cell strain excreting monoclonal antibody (MAb) resisting rice blackstreaked dwarf virus (RBSDV) and application of MAb |
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- 2013-11-26 CN CN201310605649.1A patent/CN103642938A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101633908A (en) * | 2009-09-01 | 2010-01-27 | 江苏省农业科学院 | Method for laodelphax striatellus to obtain rice black-streaked dwarf virus (RBSDV) from refrigerated diseased leaves |
| CN102533664A (en) * | 2012-01-09 | 2012-07-04 | 浙江大学 | Hybridoma cell strain excreting monoclonal antibody (MAb) resisting rice blackstreaked dwarf virus (RBSDV) and application of MAb |
Non-Patent Citations (1)
| Title |
|---|
| 王华弟等: "《水稻黑条矮缩病发生流行规律、监测预警与防控关键技术》", 《浙江农业学报》, vol. 19, no. 3, 31 December 2007 (2007-12-31), pages 141 - 146 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104830998A (en) * | 2015-05-20 | 2015-08-12 | 宋立胜 | Molecular identification method for resistance of rice black streaked dwarf virus (RBSDV) |
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