CN103642769B - 一种来源于圆弧青霉的碱性脂肪酶 - Google Patents
一种来源于圆弧青霉的碱性脂肪酶 Download PDFInfo
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- CN103642769B CN103642769B CN201310651601.4A CN201310651601A CN103642769B CN 103642769 B CN103642769 B CN 103642769B CN 201310651601 A CN201310651601 A CN 201310651601A CN 103642769 B CN103642769 B CN 103642769B
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- lipase
- present
- alkaline lipase
- enzyme
- alkaline
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/01—Carboxylic ester hydrolases (3.1.1)
- C12Y301/01003—Triacylglycerol lipase (3.1.1.3)
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
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- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
序号 | 组 分 | 重量份 |
1 | 重组碱性脂肪酶 | 40 – 80 |
2 | 碳酸钙 | 0.1– 0.8 |
3 | 硼酸钠 | 1 – 4 |
4 | 甲酸钠 | 2 – 15 |
5 | 多元醇 | 5 – 20 |
6 | 山梨酸钾 | 0.1– 0.5 |
编号 | 样品名称 | EMP A116 | EMP A117 |
1 | 蓝月亮洗衣液2 ml +1000u/ml 本发明的重组碱性脂肪酶 | 8.25 | 8.36 |
2 | 蓝月亮洗衣液2 ml +1000u/ml 竞争产品碱性脂肪酶 | 6.84 | 7.60 |
4 | 国家标准洗衣液2g(不加脂肪酶) | 2.14 | 1.62 |
Claims (5)
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CN201310651601.4A CN103642769B (zh) | 2013-12-05 | 2013-12-05 | 一种来源于圆弧青霉的碱性脂肪酶 |
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CN201310651601.4A CN103642769B (zh) | 2013-12-05 | 2013-12-05 | 一种来源于圆弧青霉的碱性脂肪酶 |
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CN103642769A CN103642769A (zh) | 2014-03-19 |
CN103642769B true CN103642769B (zh) | 2015-11-25 |
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Families Citing this family (1)
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CN112961871A (zh) * | 2021-02-26 | 2021-06-15 | 源创核新(北京)新材料科技有限公司 | 一种重组质粒、包含其的基因工程菌及其在制备碳酸二甲酯中的应用 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK37195A (da) * | 1995-04-03 | 1995-04-03 | Novo Nordisk As | Alkaline lipase |
WO2008069804A1 (en) * | 2006-12-06 | 2008-06-12 | Bunge Oils, Inc. | A continuous process and apparatus for enzymatic treatment of lipids |
CN102766612A (zh) * | 2012-06-25 | 2012-11-07 | 苏州百趣食品有限公司 | 一种利用圆弧青霉发酵生产碱性脂肪酶的方法 |
CN103387966A (zh) * | 2013-07-16 | 2013-11-13 | 中国农业大学 | 高效异源表达白圆弧青霉脂肪酶的毕赤酵母及产酶培养基 |
-
2013
- 2013-12-05 CN CN201310651601.4A patent/CN103642769B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK37195A (da) * | 1995-04-03 | 1995-04-03 | Novo Nordisk As | Alkaline lipase |
WO2008069804A1 (en) * | 2006-12-06 | 2008-06-12 | Bunge Oils, Inc. | A continuous process and apparatus for enzymatic treatment of lipids |
CN102766612A (zh) * | 2012-06-25 | 2012-11-07 | 苏州百趣食品有限公司 | 一种利用圆弧青霉发酵生产碱性脂肪酶的方法 |
CN103387966A (zh) * | 2013-07-16 | 2013-11-13 | 中国农业大学 | 高效异源表达白圆弧青霉脂肪酶的毕赤酵母及产酶培养基 |
Non-Patent Citations (6)
Title |
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AF274320.1 Penicillium cyclopium alkaline lipase mRNA, complete cds;Wu,M. et al.;《GenBank》;20000711;核苷酸序列和氨基酸序列 * |
High-level heterologous expression of an alkaline lipase gene from Penicillium cyclopium PG37 in Pichia pastoris;Zhongbiao Tan et al.;《World J Microbiol Biotechnol》;20110430;第27卷;第2767-2774页 * |
圆弧青霉PG37碱性脂肪酶的研究;邬敏辰 等;《江苏食品与发酵》;19971231;第2-7页,尤其是摘要、前言部分 * |
圆弧青霉碱性脂肪酶在毕赤酵母中的高效表达;谭中标 等;《中国生物制品学杂志》;20101130;第23卷(第11期);第1185-1189页 * |
圆弧青霉碱性脂肪酶的分离纯化和特性;邬敏辰 等;《生物化学与生物物理学报》;19991231;第31卷(第6期);第664-668页 * |
洗涤剂用酶——碱性脂肪酶的研究概述;郑毅 等;《日用化学工业》;20010228;第35-38页 * |
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