CN103636798A - Preparation method of cow-goat milk mixed-type infant formula milk powder - Google Patents
Preparation method of cow-goat milk mixed-type infant formula milk powder Download PDFInfo
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Abstract
The invention discloses a preparation method of cow-goat milk mixed-type infant formula milk powder. The preparation method of the cow-goat milk mixed-type infant formula milk powder comprises the following steps: modifying cow milk casein, and adding goat milk beta-casein and demineralized whey powder in proportion of 40:60 according to the ratio of casein to whey protein in human milk. According to the invention, the protein components and proportion are much approximate to those of breast milk, and the immunity and resistance of infant bodies can be improved obviously; the brain, bone, muscle and fat growth and the integral growth and development of infants can be accelerated; the milk powder is easier to digest and absorb; the stomach discomfort cannot be caused, and infants cannot constipate after having the milk powder; the milk power does not contain allergic foreign proteins, cannot lead to milk product allergic symptoms of diarrhea and the like, and is suitable for infants with any corporeity, especially infants who are weak in stomach and intestine and poor in corporeity.
Description
Technical field
The preparation method who the present invention relates to a kind of cattle and sheep breast mixed type baby formula milk powder, belongs to technical field of processing dairy products.
Background technology
Breast milk is the optimal wholefood of neonate, but owing to being subject to the impact of infant or mother's various factors, China still has about 30%-50% baby cannot obtain breast-feeding at present, therefore selects suitable formula milk to grow for it very important.At present commercially available baby formula milk powder is of a great variety, mostly take cow's milk as primary raw material, and the formula milk that the sheep breast of take is primary raw material is less.In fact from the angle of infant nutrient, sheep breast is rich in multiple nutritional components, more approach breast milk, especially in composition and the character of protein, essential amino acids content, nonprotein nitrogen forms, the character of fat and aliphatic acid form, the aspects such as compound sugar kind and content are better than cow's milk, lactoprotein is the most important composition in Ruzhong, lactoprotein mainly contains casein and the large class of lactalbumin 2, in sheep breast and cow's milk, caseic content does not have significant difference, in caseic kind, cow's milk be take α s-CN as main, sheep breast is maximum with β-CN content, human milk is not containing α s-CN, sheep breast is containing less α s-CN and a large amount of β-CN, so sheep breast more approaches human milk than cow's milk.
A lot of characteristics of human milk, as stronger antibacterial activity, the biologically active of defence infection ability, similar hormone function and immunologic function is all closely related with beta-casein, due to cow's milk beta-casein content seldom, the babies ' formula milk powder that the cow's milk of take is primary raw material does not just have some correlation functions of human milk.And sheep breast beta-casein not only content is approaching with human milk, and also similar breast milk of its structure, the calcium in sheep Ruzhong mainly exists with casein form, is easy to be absorbed by the body.Baby drinks and is easy to absorb, and not containing causing anaphylactogen, constipation can not occur simultaneously, and the baby, particularly stomach and intestine of applicable any physique is weak, the poor baby of physique.
Aspect babies ' formula milk powder, the research in past is all the composition that changes merely cow's milk or sheep Ruzhong albumen, or artificial some bioactivators that add, this patent is focused on the basis of original babies ' formula milk powder, invents the novel cattle and sheep breast mixed type babies ' formula milk powder of a kind of 0-3 of being suitable for a month consumption by infants.
Summary of the invention
The preparation method who the present invention relates to a kind of cattle and sheep breast mixed type baby formula milk powder, its technical scheme is as follows:
(1) from fresh milk, isolate bovine casein, obtain bovine casein and parting liquid, recycling rennin is to the bovine casein modification that is hydrolyzed;
(2) in the cow's milk parting liquid obtaining to step (1), add bovine casein, sheep breast beta-casein and desalted whey powder after modification, in adjusting formula milk, the ratio of casein and lactalbumin is 40:60;
(3) the middle gained mixed liquor of step (2) is carried out to homogeneous, sterilization and is dried obtaining Powdered original washing powder;
(4) in the Powdered original washing powder obtaining to step (3), add batching to prepare baby formula milk powder.
In described preparation method, batching is: carbohydrate, dietary fiber, aliphatic acid, vitamin and mineral matter.
Described preparation method, described batching is: energy 2016kJ/100g, carbohydrate 26.1g/100g, dietary fiber 199mg/100g, linoleic acid 1925mg/100g, alpha-linolenic acid 304mg/100g, DHA 6867 μ g/100g, arachidonic acid 13.5mg/100g, vitamin A 469 μ g/100g, vitamin D 7.1 μ g/100g, vitamin E 4.82mg/100g, vitamin K
125.2 μ g/100g, Cobastab
1355 μ g/100g, Cobastab
2372 μ g/100g, Cobastab
6>=240 μ g/100g, Cobastab
12>=1.25 μ g/100g, vitamin C 45.8mg/100g, nicotinic acid 2785 μ g/100g, folic acid 30 μ g/100g, pantothenic acid 2050 μ g/100g, compound sugar 411mg/100g, lecithin 182mg/100g, nucleotides 15mg/100g, taurine 27mg/100g, inositol 39mg/100g, l-cn 8.6mg/100g, chlorine≤902mg/100g, biotin 14.0 μ g/100g, choline 43.1mg/100g, sodium≤381mg/100g, zinc 3.41mg/100g, selenium 12.0 μ g/100g, iodine 44 μ g/100g, copper 245 μ g/100g, manganese 40～44 μ g/100g, phosphorus 451mg/100g, potassium 461mg/100g, magnesium 40.7mg/100g, calcium 561mg/100g, iron 7.42mg/100g, all the other are cow's milk solid content and fat.
Rennin described in preparation method is calf rennin.
The addition of the breast beta-casein of sheep described in described preparation method's step (2) and desalted whey powder is respectively 20g/100g～30g/100g and 30g/100g～45g/100g.
The preferred addition of described sheep breast β casein is 25g/100g, and the preferred addition of desalted whey powder is 37.5g/100g.
Described preparation method, the homogenization pressure described in step (3) is 15-20MPa, homogenizing temperature is 40-50 ℃.
Described preparation method, the sterilization temperature described in step (3) is 82-90 ℃, sterilizing time is 20-25s.
Described preparation method, concrete steps are as follows:
(1) from fresh milk, isolate bovine casein, obtain bovine casein and parting liquid, recycling rennin is to the bovine casein modification that is hydrolyzed;
(2) in the cow's milk parting liquid obtaining to step (1), add bovine casein, sheep breast beta-casein and desalted whey powder after modification, the caseic addition of described sheep breast β is 20g/100g～30g/100g, the addition of desalted whey powder is 30g/100g～45g/100g, and in adjusting formula milk, the ratio of casein and lactalbumin is 40:60;
(3) utilize high pressure to carry out homogeneous the mixed liquor of step (2) gained, homogenization pressure is 15-20MPa, and homogenizing temperature is 40-50 ℃;
(4) control sterilization temperature at 82-90 ℃, sterilizing time is 20-25s;
(5) adopt many shower nozzles of pressure downflow type drying tower to spray dry, obtain Powdered original washing powder;
(6) carbohydrate, dietary fiber, aliphatic acid, vitamin and mineral matter are mixed with the Powdered original washing powder of step (5) gained, obtain baby formula milk powder;
(7) packing.
Further, above-mentioned preparation method's concrete steps are as follows:
(1) bovine casein modification: isolate bovine casein from fresh milk, obtain bovine casein and parting liquid, recycling rennin is to the casein modification that is hydrolyzed;
(2) adjust protein ratio: in the cow's milk parting liquid obtaining to step (1), add bovine casein, sheep breast beta-casein and desalted whey powder after modification, the caseic addition of described sheep breast β is 25g/100g, the addition of desalted whey powder is 37.5g/100g, and in adjusting formula milk, the ratio of casein and lactalbumin is 40:60;
(3) homogeneous: utilize high pressure to carry out homogeneous the mixed liquor of step (2) gained, homogenization pressure is 16MPa, and homogenizing temperature is 45 ℃;
(4) sterilization: control sterilization temperature at 85 ℃, sterilizing time is 24s;
(5) spraying is dry: adopt many shower nozzles of pressure downflow type drying tower to spray dry, obtain Powdered original washing powder;
(6) be dry mixed: carbohydrate, dietary fiber, aliphatic acid, vitamin, mineral matter and other nutrients are mixed with the Powdered original washing powder of step (5) gained, obtain baby formula milk powder;
(7) packing: after formula milk exhaust thoroughly, tightly seal.
Beneficial effect of the present invention: the albumen of formula milk provided by the invention forms and ratio all more approaches breast milk.Index and spleen index experiment shows that this Novel baby formula milk can significantly improve immunity and the resistance of baby's body.The more common babies ' formula milk powder of this Novel baby formula milk of the demonstration of mouse swimming test data has more anti-fatigue ability.The checking of the bone growth experiment by rat, data show that this Novel baby formula milk has outstanding performance aspect bone and whole growing promoting.By the experiment of hydrolyzed bovine milk solid and interpolation goat milk beta-casein, test, removed Ruzhong and can cause the irritated caseic impact of foreign preteins α s-, experimental result shows that novel infant formula milk powder can effectively avoid diarrhoea to wait the generation of dairy products allergic symptom.
Accompanying drawing explanation
Fig. 1 bovine casein modification electrophoresis comparison diagram.
The tomographic map of Fig. 2 DEAE-Sepharose Fast Flow purifying sheep breast beta-casein.
Fig. 3 sheep breast beta-casein DEAE-Sepharose Fast Flow anion-exchange chromatography eluting peak SDS-PAGE analysis chart; (M:Marker; 1: peak I; 2: peak II sheep breast beta-casein; 3: peak III; 4: peak IV).
The impact of the different milk powder of Fig. 4 on Rat Skeletal growth.
The specific embodiment
Material and reagent
Fresh goat milk, picks up from Suiling County, Heilongjiang Province Fu Wang milch goat plant; Fresh milk, picks up from cattle farm, Song Hua River, Harbin.Healthy female Kunming one-level small white mouse, in 45-60 days ages, 18-20g, is ground and is provided by Kazakhstan beast.Urea, Solarbio company; Sigma company; Trypsase, Sigma company; Sodium chloride, Ke Miou chemical reagent development centre, Tianjin; Hydrochloric acid, Ke Miou chemical reagent development centre, Tianjin; 95% ethanol (v/v), Mei Lai Bioisystech Co., Ltd; Ammonium persulfate (AP), Ke Miou chemical reagent development centre, Tianjin.
Instrument and equipment
Tris, glycine, dodecyl sodium sulfate (SDS), beta-mercaptoethanol (2-ME), N, it is pure that N '-methylene-bisacrylamide, acrylamide, glycerine, Coomassie brilliant blue (CBB) R-250, ammonium persulfate (AP), bromophenol blue, hydrochloric acid, volume fraction are 95% ethanol, methyl alcohol, glacial acetic acid is analysis; Urea, sodium chloride and DEAE-Sepharose Fast Flow; Ultra-pure water.
GL-21M high speed freezing centrifuge; DELTA320pH meter; PL2002 electronic balance; Small frozen drying machine; DYY-10C type electrophoresis apparatus; AKTA purifer100 protein purification instrument; Ziptip C18 chromatographic column.
Embodiment 1: the modification of bovine casein
(1) extraction of rennin and vitality test
Calf abomasum → clean → fritter → lixiviate (dividing three times) → filter → dialysis → dry → rennin rennin vitality test is referring to Wuxi enzyme preparation factory product description.
(2) separation of bovine casein
By the centrifugal degrease of fresh milk, with hydrochloric acid, regulate cow's milk pH to 4.3, collecting precipitation, is casein.
(3) caseic people's emulsification modification and Optimal Experimental
The casein precipitation that previous step is collected is dissolved.
Casein solution → rennin effect → enzyme work → the centrifugation of going out, supernatant is the casein of modification, use SDS-PAGE detected through gel electrophoresis, find to compare with former casein, the electrophoretic band of α s-casein position has weakened many, and the electrophoretic band intensity of β casein position does not reduce relatively.This explanation, the casein after people's emulsification modification has successfully been removed a large amount of α s-caseins, has retained at the same time most β casein.
From the above results, in the cow's milk solid content of modification, by a large amount of in cow's milk and exist hardly in human milk, and can become the α s-casein hydrolysis that baby digests and assimilates cow's milk major obstacle and fallen, and retain the major protein beta-casein in human milk and be not hydrolyzed.
Embodiment 2: the separation and purification of sheep breast beta-casein
(1) degrease
Select fresh goat dairy sample (10mL) at 4 ℃ of centrifugal 30min of 10400g, sample is divided into three layers, and wherein butterfat part is on upper strata, and whey portion is in middle level, and breast grain part, in bottom, is avoided upper strata lipid layer, draws whey portion standby.
(2) separation of beta-casein
Whey pH is adjusted to 4.6, reaches casein isoelectric point, 4 ℃, 40000g, centrifugal 30min, collecting precipitation, claims 100mg casein to be precipitated and dissolved in the level pad (Tris of 20mmol/L, the urea of 3.3mol/L) of 50mL, 1M NaOH regulates pH to 9.5.Sample discontinuity in 20 ℃ of water-baths stirs 0.5～1h.After fully dissolving, 4 ℃, the centrifugal 30min of 10000g, the membrane filtration of 0.45 μ m for supernatant, crosses DEAE-Sepharose Fast Flow ion exchange column.The protein being adsorbed with 0.3mol/L NaCl continuous gradient wash-out, collects A
280nmthe eluting peak at place.By the purity of SDS-PAGE electrophoretic determination eluting peak, according to character and document, determine that peak 2 is for sheep breast beta-casein and to its postlyophilization of dialysing.
From the above results, sheep breast beta-casein can carry out separation in vitro, has removed and has easily caused baby's Disorder of Digestion and A orption and irritated alpha-casein, after baby drinks, constipation can not occur, and is applicable to the baby of any physique.
Embodiment 3: the interpolation of desalted whey powder
The ratio of casein and lactalbumin in human milk simulating, artificially adds desalted whey powder, and making the ratio of casein and lactalbumin in formula milk is 40:60.
Embodiment 4: preparation method
The caseic addition of sheep breast β of take is 25g/100g, and the addition of desalted whey powder is that 37.5g/100g is example, preparation method, and concrete steps are as follows:
(1) from fresh milk, isolate bovine casein, obtain bovine casein and parting liquid, recycling rennin is to the bovine casein modification that is hydrolyzed;
(2) in the cow's milk parting liquid obtaining to step (1), add bovine casein, sheep breast beta-casein and desalted whey powder after modification, the caseic addition of described sheep breast β is 25g/100g, the addition of desalted whey powder is 37.5g/100g, and in adjusting formula milk, the ratio of casein and lactalbumin is 40:60;
(3) utilize high pressure to carry out homogeneous the mixed liquor of step (2) gained, homogenization pressure is 16MPa, and homogenizing temperature is 45 ℃;
(4) control sterilization temperature at 85 ℃, sterilizing time is 24s;
(5) adopt many shower nozzles of pressure downflow type drying tower to spray dry, obtain Powdered original washing powder;
(6) carbohydrate, dietary fiber, aliphatic acid, vitamin and mineral matter are mixed with the Powdered original washing powder of step (5) gained, obtain baby formula milk powder;
(7) packing.
Embodiment 5: a kind of babies ' formula milk powder
A baby formula milk powder carries out modification by bovine casein on the basis of original baby formula, adds the desalted whey powder of sheep breast beta-casein and cow's milk, the content of casein and lactalbumin in human milk simulating, and the ratio that regulates the two is 40:60.
A kind of formula of baby formula milk powder is: energy 2016kJ/100g, carbohydrate 26.1g/100g, dietary fiber 199mg/100g, linoleic acid 1925mg/100g, alpha-linolenic acid 304mg/100g, DHA 6867 μ g/100g, arachidonic acid 13.5mg/100g, vitamin A 469 μ g/100g, vitamin D 7.1 μ g/100g, vitamin E 4.82mg/100g, vitamin K
125.2 μ g/100g, Cobastab
1355 μ g/100g, Cobastab
2372 μ g/100g, Cobastab
6>=240 μ g/100g, Cobastab
12>=1.25 μ g/100g, vitamin C 45.8mg/100g, nicotinic acid 2785 μ g/100g, folic acid 30 μ g/100g, pantothenic acid 2050 μ g/100g, compound sugar 411mg/100g, lecithin 182mg/100g, nucleotides 15mg/100g, taurine 27mg/100g, inositol 39mg/100g, l-cn 8.6mg/100g, chlorine≤902mg/100g, biotin 14.0 μ g/100g, choline 43.1mg/100g, sodium≤381mg/100g, zinc 3.41mg/100g, selenium 12.0 μ g/100g, iodine 44 μ g/100g, copper 245 μ g/100g, manganese 40～44 μ g/100g, phosphorus 451mg/100g, potassium 461mg/100g, magnesium 40.7mg/100g, calcium 561mg/100g, iron 7.42mg/100g, added again sheep breast beta-casein 20g/100g, desalted whey powder 30g/100g, all the other are milk solids and fat after modification.
Embodiment 6
A kind of formula of baby formula milk powder is: energy 2016kJ/100g, carbohydrate 26.1g/100g, dietary fiber 199mg/100g, linoleic acid 1925mg/100g, alpha-linolenic acid 304mg/100g, DHA 6867 μ g/100g, arachidonic acid 13.5mg/100g, vitamin A 469 μ g/100g, vitamin D 7.1 μ g/100g, vitamin E 4.82mg/100g, vitamin K
125.2 μ g/100g, Cobastab
1355 μ g/100g, Cobastab
2372 μ g/100g, Cobastab
6>=240 μ g/100g, Cobastab
12>=1.25 μ g/100g, vitamin C 45.8mg/100g, nicotinic acid 2785 μ g/100g, folic acid 30 μ g/100g, pantothenic acid 2050 μ g/100g, compound sugar 411mg/100g, lecithin 182mg/100g, nucleotides 15mg/100g, taurine 27mg/100g, inositol 39mg/100g, l-cn 8.6mg/100g, chlorine≤902mg/100g, biotin 14.0 μ g/100g, choline 43.1mg/100g, sodium≤381mg/100g, zinc 3.41mg/100g, selenium 12.0 μ g/100g, iodine 44 μ g/100g, copper 245 μ g/100g, manganese 40～44 μ g/100g, phosphorus 451mg/100g, potassium 461mg/100g, magnesium 40.7mg/100g, calcium 561mg/100g, iron 7.42mg/100g, added again sheep breast beta-casein 22.5g/100g, all the other are milk solids and fat after modification for desalted whey 33.75g/100g.
Embodiment 7
A kind of formula of baby formula milk powder is: energy 2016kJ/100g, carbohydrate 26.1g/100g, dietary fiber 199mg/100g, linoleic acid 1925mg/100g, alpha-linolenic acid 304mg/100g, DHA 6867 μ g/100g, arachidonic acid 13.5mg/100g, vitamin A 469 μ g/100g, vitamin D 7.1 μ g/100g, vitamin E 4.82mg/100g, vitamin K
125.2 μ g/100g, Cobastab
1355 μ g/100g, Cobastab
2372 μ g/100g, Cobastab
6>=240 μ g/100g, Cobastab
12>=1.25 μ g/100g, vitamin C 45.8mg/100g, nicotinic acid 2785 μ g/100g, folic acid 30 μ g/100g, pantothenic acid 2050 μ g/100g, compound sugar 411mg/100g, lecithin 182mg/100g, nucleotides 15mg/100g, taurine 27mg/100g, inositol 39mg/100g, l-cn 8.6mg/100g, chlorine≤902mg/100g, biotin 14.0 μ g/100g, choline 43.1mg/100g, sodium≤381mg/100g, zinc 3.41mg/100g, selenium 12.0 μ g/100g, iodine 44 μ g/100g, copper 245 μ g/100g, manganese 40～44 μ g/100g, phosphorus 451mg/100g, potassium 461mg/100g, magnesium 40.7mg/100g, calcium 561mg/100g, iron 7.42mg/100g, added again sheep breast beta-casein 25g/100g, all the other are milk solids and fat after modification for desalted whey 37.5g/100g.
Embodiment 8
A kind of formula of baby formula milk powder is: energy 2016kJ/100g, carbohydrate 26.1g/100g, dietary fiber 199mg/100g, linoleic acid 1925mg/100g, alpha-linolenic acid 304mg/100g, DHA 6867 μ g/100g, arachidonic acid 13.5mg/100g, vitamin A 469 μ g/100g, vitamin D 7.1 μ g/100g, vitamin E 4.82mg/100g, vitamin K
125.2 μ g/100g, Cobastab
1355 μ g/100g, Cobastab
2372 μ g/100g, Cobastab
6>=240 μ g/100g, Cobastab
12>=1.25 μ g/100g, vitamin C 45.8mg/100g, nicotinic acid 2785 μ g/100g, folic acid 30 μ g/100g, pantothenic acid 2050 μ g/100g, compound sugar 411mg/100g, lecithin 182mg/100g, nucleotides 15mg/100g, taurine 27mg/100g, inositol 39mg/100g, l-cn 8.6mg/100g, chlorine≤902mg/100g, biotin 14.0 μ g/100g, choline 43.1mg/100g, sodium≤381mg/100g, zinc 3.41mg/100g, selenium 12.0 μ g/100g, iodine 44 μ g/100g, copper 245 μ g/100g, manganese 40～44 μ g/100g, phosphorus 451mg/100g, potassium 461mg/100g, magnesium 40.7mg/100g, calcium 561mg/100g, iron 7.42mg/100g, added again sheep breast beta-casein 27.5g/100g, desalted whey powder 41.25g/100g, all the other are milk solids and fat after modification.
Embodiment 9
A kind of formula of baby formula milk powder is: energy 2016kJ/100g, carbohydrate 26.1g/100g, dietary fiber 199mg/100g, linoleic acid 1925mg/100g, alpha-linolenic acid 304mg/100g, DHA 6867 μ g/100g, arachidonic acid 13.5mg/100g, vitamin A 469 μ g/100g, vitamin D 7.1 μ g/100g, vitamin E 4.82mg/100g, vitamin K
125.2 μ g/100g, Cobastab
1355 μ g/100g, Cobastab
2372 μ g/100g, Cobastab
6>=240 μ g/100g, Cobastab
12>=1.25 μ g/100g, vitamin C 45.8mg/100g, nicotinic acid 2785 μ g/100g, folic acid 30 μ g/100g, pantothenic acid 2050 μ g/100g, compound sugar 411mg/100g, lecithin 182mg/100g, nucleotides 15mg/100g, taurine 27mg/100g, inositol 39mg/100g, l-cn 8.6mg/100g, chlorine≤902mg/100g, biotin 14.0 μ g/100g, choline 43.1mg/100g, sodium≤381mg/100g, zinc 3.41mg/100g, selenium 12.0 μ g/100g, iodine 44 μ g/100g, copper 245 μ g/100g, manganese 40～44 μ g/100g, phosphorus 451mg/100g, potassium 461mg/100g, magnesium 40.7mg/100g, calcium 561mg/100g, iron 7.42mg/100g, added again sheep breast beta-casein 30g/100g, desalted whey powder 45g/100g, all the other are milk solids and fat after modification.
Get the small white mouse (entirely female) in 45～60 day age, be divided into control group, test group 1, test 2,3 groups, 10 every group, totally 30, only gavage respectively physiological saline, ordinary powdered milk, embodiment 6 Novel baby formula milk (concentration is 1g/ml) 4mL/, once a day, continuous use 10d, then puts to death small white mouse by control group experimental group 1 experimental group 2 use vertebra dislocation methods, weigh in, take out spleen and weigh.The index and spleen index that is compared to spleen weight/mg and body weight/g.Different preparations are as shown in table 1 on the impact of mouse spleen index.
The impact of the different milk powder of table 1 on mouse spleen index
As shown in table 1, through data analysis, to find, the index and spleen index difference between 2 groups of control group and tests is (p<0.01) extremely significantly; Index and spleen index between control group and test group 1 has significant difference (p<0.05).
From above experiment, add common babies ' formula milk powder in feed after, receive with new formulation milk powder, all can significantly improve the index and spleen index of mouse, but after adding new formulation milk powder, index and spleen index increase is more, illustrates that the more common babies ' formula milk powder of this new formulation milk powder more can improve immunity.
Embodiment 11 new formulation milk powder antifatigue experiments
Swimming test is by small white mouse according to the continuous gavage 10 days of table 1, and 60min after last gavage, weighs in respectively.Then the weight of 2.0g is lain in to small white mouse root of the tail portion, then small white mouse is placed in to the pond went swimming of 18.5 ℃ of 45cm * 30cm * 20cm, water temperature, start timing.When small white mouse head submerged 10s can not emerge, be muscle power exhaustion, timing at once, is small white mouse swimming time, and different preparations are as shown in table 2 on the impact of mouse swimming time.
The impact of the different milk powder of table 2 on mouse swimming time
Above description of test, the more common babies ' formula milk powder of this Novel baby formula milk is significantly improved aspect antifatigue.
Embodiment 12 new formulation milk powder promote skeleton development experiment
Choose 3 week age clean level pure lines male SD rat and feed after 4 weeks with normal diet, fill with by force and feed 0.8g/d, gavage 10 days.Control group is the experimental group of gavage physiological saline, and experimental group 1 and experimental group 2 are respectively the experimental group of gavage regular convention formula milk powder and Novel baby formula milk.Gavage the 6th day, in abdominal cavity, inject fluorescence matrix-tetracycline, within the 8th day, carry out the 2nd injection of fluorescence matrix-tetracycline, within the 10th day, anesthetized rat is lethal, the complete shin bone that separates, by the distance between vernier caliper measurement proximal tibia articular surface and distal joint face, is the shin bone length of feeding 10d, get the mean value of measuring for its 3 times, result as shown in Figure 4.Fig. 4 shows that control group 0 shin bone growth length is 65 μ m, and experimental group 1 shin bone growth length is 75 μ m, and experimental group 2 shin bone growth lengths are 82 μ m, and the performance aspect promotion skeleton development of Novel baby formula milk is remarkable.
Claims (10)
1. a preparation method for cattle and sheep breast mixed type baby formula milk powder, is characterized in that, method is as follows:
(1) from fresh milk, isolate bovine casein, obtain bovine casein and parting liquid, recycling rennin is to the bovine casein modification that is hydrolyzed;
(2) in the cow's milk parting liquid obtaining to step (1), add bovine casein, sheep breast beta-casein and desalted whey powder after modification, in adjusting formula milk, the ratio of casein and lactalbumin is 40:60;
(3) the middle gained mixed liquor of step (2) is carried out to homogeneous, sterilization and is dried obtaining Powdered original washing powder;
(4) in the Powdered original washing powder obtaining to step (3), add batching to prepare baby formula milk powder.
2. preparation method claimed in claim 1, is characterized in that, described batching is: carbohydrate, dietary fiber, aliphatic acid, vitamin and mineral matter.
3. preparation method claimed in claim 1, it is characterized in that, described batching is: energy 2016kJ/100g, carbohydrate 26.1g/100g, dietary fiber 199mg/100g, linoleic acid 1925mg/100g, alpha-linolenic acid 304mg/100g, DHA 6867 μ g/100g, arachidonic acid 13.5mg/100g, vitamin A 469 μ g/100g, vitamin D 7.1 μ g/100g, vitamin E 4.82mg/100g, vitamin K
125.2 μ g/100g, Cobastab
1355 μ g/100g, Cobastab
2372 μ g/100g, Cobastab
6>=240 μ g/100g, Cobastab
12>=1.25 μ g/100g, vitamin C 45.8mg/100g, nicotinic acid 2785 μ g/100g, folic acid 30 μ g/100g, pantothenic acid 2050 μ g/100g, compound sugar 411mg/100g, lecithin 182mg/100g, nucleotides 15mg/100g, taurine 27mg/100g, inositol 39mg/100g, l-cn 8.6mg/100g, chlorine≤902mg/100g, biotin 14.0 μ g/100g, choline 43.1mg/100g, sodium≤381mg/100g, zinc 3.41mg/100g, selenium 12.0 μ g/100g, iodine 44 μ g/100g, copper 245 μ g/100g, manganese 40～44 μ g/100g, phosphorus 451mg/100g, potassium 461mg/100g, magnesium 40.7mg/100g, calcium 561mg/100g, iron 7.42mg/100g, all the other are cow's milk solid content and fat.
4. preparation method claimed in claim 1, is characterized in that, the rennin described in step (1) is calf rennin.
5. preparation method claimed in claim 1, is characterized in that, the addition of the breast beta-casein of sheep described in step (2) and desalted whey powder is respectively 20g/100g～30g/100g and 30g/100g～45g/100g.
6. preparation method claimed in claim 3, is characterized in that, the caseic addition of described sheep breast β is 25g/100g, and the addition of desalted whey powder is 37.5g/100g.
7. preparation method claimed in claim 1, is characterized in that, the homogenization pressure described in step (3) is 15-20MPa, and homogenizing temperature is 40-50 ℃.
8. preparation method claimed in claim 1, is characterized in that, the sterilization temperature described in step (3) is 82-90 ℃, and sterilizing time is 20-25s.
9. preparation method claimed in claim 1, is characterized in that, step is as follows:
(1) from fresh milk, isolate bovine casein, obtain bovine casein and parting liquid, recycling rennin is to the bovine casein modification that is hydrolyzed;
(2) in the cow's milk parting liquid obtaining to step (1), add bovine casein, sheep breast beta-casein and desalted whey powder after modification, the caseic addition of described sheep breast β is 20g/100g～30g/100g, the addition of desalted whey powder is 30g/100g～45g/100g, and in adjusting formula milk, the ratio of casein and lactalbumin is 40:60;
(3) utilize high pressure to carry out homogeneous the mixed liquor of step (2) gained, homogenization pressure is 15-20MPa, and homogenizing temperature is 40-50 ℃;
(4) control sterilization temperature at 82-90 ℃, sterilizing time is 20-25s;
(5) adopt many shower nozzles of pressure downflow type drying tower to spray dry, obtain Powdered original washing powder;
(6) carbohydrate, dietary fiber, aliphatic acid, vitamin and mineral matter are mixed with the Powdered original washing powder of step (5) gained, obtain baby formula milk powder;
(7) packing.
10. preparation method claimed in claim 7, is characterized in that, concrete steps are as follows:
(1) bovine casein modification: isolate bovine casein from fresh milk, obtain bovine casein and parting liquid, recycling rennin is to the casein modification that is hydrolyzed;
(2) adjust protein ratio: in the cow's milk parting liquid obtaining to step (1), add bovine casein, sheep breast beta-casein and desalted whey powder after modification, the caseic addition of described sheep breast β is 25g/100g, the addition of desalted whey powder is 37.5g/100g, and in adjusting formula milk, the ratio of casein and lactalbumin is 40:60;
(3) homogeneous: utilize high pressure to carry out homogeneous the mixed liquor of step (2) gained, homogenization pressure is 16MPa, and homogenizing temperature is 45 ℃;
(4) sterilization: control sterilization temperature at 85 ℃, sterilizing time is 24s;
(5) spraying is dry: adopt many shower nozzles of pressure downflow type drying tower to spray dry, obtain Powdered original washing powder;
(6) be dry mixed: carbohydrate, dietary fiber, aliphatic acid, vitamin, mineral matter and other nutrients are mixed with the Powdered original washing powder of step (5) gained, obtain baby formula milk powder;
(7) packing: after formula milk exhaust thoroughly, tightly seal.
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| CN104012659A (en) * | 2014-06-26 | 2014-09-03 | 中恩（天津）营养科技有限公司 | Formula powder for phenylketonuria children and preparation method thereof |
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