CN103636798B - A kind of preparation method of cow-goat milk mixed-type infant formula milk powder - Google Patents
A kind of preparation method of cow-goat milk mixed-type infant formula milk powder Download PDFInfo
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Abstract
The invention discloses a kind of preparation method of cow-goat milk mixed-type infant formula milk powder, is that bovine casein is carried out modification, according to the ratio of casein in human milk and lactalbumin, adds sheep breast beta-casein and desalted whey powder according to the ratio of 40:60.Albumen composition of the present invention and ratio are all closer to breast milk, immunity and the resistance of baby's body can be significantly improved, promote growing of the brain of baby, bone, muscle, fat growth and entirety, absorption more easy to digest, can not cause have a stomach upset, can not there is constipation after drinking in baby, containing causing irritated foreign preteins, can not produce the generation of allergic symptom such as dairy products such as diarrhoea grade, be applicable to the baby that baby, particularly stomach and intestine are more weak, physique is poor of any physique.
Description
Technical field
The present invention relates to a kind of preparation method of cow-goat milk mixed-type infant formula milk powder, belong to technical field of processing dairy products.
Background technology
Breast milk is the optimal wholefood of neonate, but due to the impact of the various factors that is subject to infant or mother, China still has the baby of about 30%-50% to obtain breast-feeding at present, therefore selects the formula milk be suitable for grow very important for it.Baby formula milk powder commercially available is at present of a great variety, is mostly primary raw material with cow's milk, with sheep breast for the formula milk of primary raw material is less.In fact from the angle of infant nutrient, sheep breast is rich in multiple nutritional components, closer to breast milk, especially in composition and the character of protein, essential amino acids content, nonprotein nitrogen forms, the character of fat and aliphatic acid composition, the aspects such as compound sugar kind and content are better than cow's milk, lactoprotein is the most important composition in Ruzhong, lactoprotein mainly contains casein and the large class of lactalbumin 2, sheep breast and cow's milk in caseic content there is no significant difference, in caseic kind, cow's milk is based on α s-CN, sheep breast is then maximum with β-CN content, human milk is not containing α s-CN, sheep breast is containing less α s-CN and a large amount of β-CN, so sheep breast than cow's milk closer to human milk.
A lot of characteristics of human milk, as stronger antibacterial activity, the biologically active of defence infection ability, similar hormone function and immunologic function is all closely related with beta-casein, because cow's milk beta-casein content is little, be that the babies ' formula milk powder of primary raw material does not just have some correlation functions of human milk with cow's milk.And sheep breast beta-casein not only content and human milk are close, and its structure also similar breast milk, the calcium in sheep Ruzhong, mainly with the existence of casein form, is easy to be absorbed by the body.Baby drinks and is easy to absorb, and simultaneously not containing causing anaphylactogen, constipation can not occur, be applicable to the baby that baby, particularly stomach and intestine are more weak, physique is poor of any physique.
In babies ' formula milk powder, the research in past is all the composition changing merely cow's milk or sheep Ruzhong albumen, or artificial adds some bioactivators, this patent is focused on the basis of original babies ' formula milk powder, invents a kind of novel cattle and sheep breast mixed type babies ' formula milk powder being suitable for 0-3 month consumption by infants.
Summary of the invention
The present invention relates to a kind of preparation method of cow-goat milk mixed-type infant formula milk powder, its technical scheme is as follows:
(1) from fresh milk, isolate bovine casein, obtain bovine casein and parting liquid, recycling rennin to be hydrolyzed modification to bovine casein;
(2) add modified bovine casein, sheep breast beta-casein and desalted whey powder in the cow's milk parting liquid obtained to step (1), the ratio regulating casein and lactalbumin in formula milk is 40:60;
(3) gained mixed liquor in step (2) is carried out homogeneous, sterilization drying obtains Powdered original washing powder;
(4) add batching in the Powdered original washing powder obtained to step (3) and prepare baby formula milk powder.
In described preparation method, batching is: carbohydrate, dietary fiber, aliphatic acid, vitamin and mineral matter.
Described preparation method, described batching is: energy 2016kJ/100g, carbohydrate 26.1g/100g, dietary fiber 199mg/100g, linoleic acid 1925mg/100g, alpha-linolenic acid 304mg/100g, DHA 6867 μ g/100g, arachidonic acid 13.5mg/100g, vitamin A 469 μ g/100g, vitamin D 7.1 μ g/100g, vitamin E 4.82mg/100g, vitamin K
125.2 μ g/100g, Cobastab
1355 μ g/100g, Cobastab
2372 μ g/100g, Cobastab
6>=240 μ g/100g, Cobastab
12>=1.25 μ g/100g, vitamin C 45.8mg/100g, nicotinic acid 2785 μ g/100g, folic acid 30 μ g/100g, pantothenic acid 2050 μ g/100g, compound sugar 411mg/100g, lecithin 182mg/100g, nucleotides 15mg/100g, taurine 27mg/100g, inositol 39mg/100g, l-cn 8.6mg/100g, chlorine≤902mg/100g, biotin 14.0 μ g/100g, choline 43.1mg/100g, sodium≤381mg/100g, zinc 3.41mg/100g, selenium 12.0 μ g/100g, iodine 44 μ g/100g, copper 245 μ g/100g, manganese 40 ~ 44 μ g/100g, phosphorus 451mg/100g, potassium 461mg/100g, magnesium 40.7mg/100g, calcium 561mg/100g, iron 7.42mg/100g, all the other are cow's milk solid content and fat.
Rennin described in preparation method is calf rennin.
The addition of the breast beta-casein of sheep described in described preparation method's step (2) and desalted whey powder is respectively 20g/100g ~ 30g/100g and 30g/100g ~ 45g/100g.
The preferred addition of described sheep breast β casein is 25g/100g, and the preferred addition of desalted whey powder is 37.5g/100g.
Described preparation method, the homogenization pressure described in step (3) is 15-20MPa, and homogenizing temperature is 40-50 DEG C.
Described preparation method, the sterilization temperature described in step (3) is 82-90 DEG C, and sterilizing time is 20-25s.
Described preparation method, concrete steps are as follows:
(1) from fresh milk, isolate bovine casein, obtain bovine casein and parting liquid, recycling rennin to be hydrolyzed modification to bovine casein;
(2) modified bovine casein, sheep breast beta-casein and desalted whey powder is added in the cow's milk parting liquid obtained to step (1), the caseic addition of described sheep breast β is 20g/100g ~ 30g/100g, the addition of desalted whey powder is 30g/100g ~ 45g/100g, and the ratio regulating casein and lactalbumin in formula milk is 40:60;
(3) utilize high pressure to carry out homogeneous the mixed liquor of step (2) gained, homogenization pressure is 15-20MPa, and homogenizing temperature is 40-50 DEG C;
(4) control sterilization temperature at 82-90 DEG C, sterilizing time is 20-25s;
(5) adopt pressure downflow type many shower nozzles drying tower to carry out spraying dry, obtain Powdered original washing powder;
(6) carbohydrate, dietary fiber, aliphatic acid, vitamin and the mineral matter Powdered original washing powder with step (5) gained is mixed, obtain baby formula milk powder;
(7) pack.
Further, the concrete steps of above-mentioned preparation method are as follows:
(1) bovine casein modification: isolate bovine casein from fresh milk, obtains bovine casein and parting liquid, and recycling rennin to be hydrolyzed modification to casein;
(2) protein ratio is adjusted: in the cow's milk parting liquid that step (1) obtains, add modified bovine casein, sheep breast beta-casein and desalted whey powder, the caseic addition of described sheep breast β is 25g/100g, the addition of desalted whey powder is 37.5g/100g, and the ratio regulating casein and lactalbumin in formula milk is 40:60;
(3) homogeneous: utilize high pressure to carry out homogeneous the mixed liquor of step (2) gained, homogenization pressure is 16MPa, and homogenizing temperature is 45 DEG C;
(4) sterilization: control sterilization temperature at 85 DEG C, sterilizing time is 24s;
(5) spraying dry: adopt pressure downflow type many shower nozzles drying tower to carry out spraying dry, obtain Powdered original washing powder;
(6) be dry mixed: carbohydrate, dietary fiber, aliphatic acid, vitamin, mineral matter and other nutrients Powdered original washing powder with step (5) gained is mixed, obtain baby formula milk powder;
(7) pack: after formula milk exhaust thoroughly, tightly seal.
Beneficial effect of the present invention: the albumen composition of formula milk provided by the invention and ratio are all closer to breast milk.Index and spleen index experiment shows that this Novel baby formula milk can significantly improve immunity and the resistance of baby's body.The more common babies ' formula milk powder of this Novel baby formula milk of the display of mouse swimming test data has more anti-fatigue ability.By the checking that the bone growth of rat is tested, data show that this Novel baby formula milk is promoting to have outstanding performance in the growing of bone and entirety.Tested by the experiment of hydrolyzed bovine milk solid and interpolation goat milk beta-casein, eliminate Ruzhong and can cause the irritated caseic impact of foreign preteins α s-, experimental result shows that novel infant formula milk powder can effectively avoid diarrhoea to wait the generation of dairy products allergic symptom.
Accompanying drawing explanation
Fig. 1 bovine casein modification electrophoresis comparison diagram.
The tomographic map of Fig. 2 DEAE-Sepharose Fast Flow purifying sheep breast beta-casein.
Fig. 3 sheep breast beta-casein DEAE-Sepharose Fast Flow anion-exchange chromatography eluting peak SDS-PAGE analysis chart; (M:Marker; 1: peak I; 2: peak II sheep breast beta-casein; 3: peak III; 4: peak IV).
The impact that the different milk powder of Fig. 4 grows Rat Skeletal.
Detailed description of the invention
Material and reagent
Fresh goat milk, picks up from Suiling County, Heilongjiang Province Fu Wang milch goat plant; Fresh milk, picks up from cattle farm, Song Hua River, Harbin.Healthy female Kunming one-level small white mouse, in 45-60 days ages, 18-20g, is ground by Kazakhstan beast and provides.Urea, Solarbio company; Sigma company; Trypsase, Sigma company; Sodium chloride, Ke Miou chemical reagent development centre, Tianjin; Hydrochloric acid, Ke Miou chemical reagent development centre, Tianjin; 95% ethanol (v/v), Mei Lai Bioisystech Co., Ltd; Ammonium persulfate (AP), Ke Miou chemical reagent development centre, Tianjin.
Instrument and equipment
Tris, glycine, dodecyl sodium sulfate (SDS), beta-mercaptoethanol (2-ME), N, N '-methylene-bisacrylamide, acrylamide, glycerine, Coomassie brilliant blue (CBB) R-250, ammonium persulfate (AP), bromophenol blue, hydrochloric acid, volume fraction are 95% ethanol, methyl alcohol, glacial acetic acid be analyze pure; Urea, sodium chloride and DEAE-Sepharose Fast Flow; Ultra-pure water.
GL-21M high speed freezing centrifuge; DELTA320pH counts; PL2002 electronic balance; Small frozen drying machine; DYY-10C type electrophoresis apparatus; AKTA purifer100 protein purification instrument; Ziptip C18 chromatographic column.
Embodiment 1: the modification of bovine casein
(1) extraction of rennin and vitality test
Calf abomasum → clean → fritter → lixiviate (dividing three times) → filtration → dialysis → drying → rennin rennin vitality test is see Wuxi enzyme preparation factory product description.
(2) separation of bovine casein
By centrifugal for fresh milk degrease, regulate cow's milk pH to 4.3 with hydrochloric acid, collecting precipitation, is casein.
(3) caseic humanized modification and Optimal Experimental
Casein precipitate previous step collected dissolves.
Casein solution → rennin effect → enzyme work → centrifugation of going out, supernatant is the casein of modification, use SDS-PAGE detected through gel electrophoresis, find to compare with former casein, the electrophoretic band of α s-casein position reduces many, and the electrophoretic band intensity of β casein position does not reduce relatively.This illustrates, successfully eliminates a large amount of α s-caseins, remain most β casein at the same time through humanized modified casein.
From the above results, in the cow's milk solid content of modification, by a large amount of in cow's milk and exist hardly in human milk, and the α s-casein hydrolysis that baby digests and assimilates cow's milk major obstacle can be become fallen, and the major protein beta-casein remained in human milk is not hydrolyzed.
Embodiment 2: the separation and purification of sheep breast beta-casein
(1) degrease
Select fresh goat milk sample (10mL) at 4 DEG C of centrifugal 30min of 10400g, sample is divided into three layers, and wherein butterfat part is on upper strata, and whey portion is in middle level, and newborn grain part, in bottom, avoids upper strata lipid layer, draws whey portion for subsequent use.
(2) separation of beta-casein
Whey pH is adjusted to 4.6, reaches casein isoelectric point, 4 DEG C, 40000g, centrifugal 30min, collecting precipitation, claim 100mg casein precipitate to be dissolved in the level pad (urea of the Tris of 20mmol/L, 3.3mol/L) of 50mL, 1M NaOH regulates pH to 9.5.Sample discontinuity in 20 DEG C of water-baths stirs 0.5 ~ 1h.After abundant dissolving, 4 DEG C, the centrifugal 30min of 10000g, the supernatant membrane filtration of 0.45 μm, crosses DEAE-Sepharose Fast Flow ion exchange column.With 0.3mol/L NaCl continuous gradient wash-out by the protein adsorbed, collect A
280nmthe eluting peak at place.By the purity of SDS-PAGE electrophoretic determination eluting peak, according to character and document, determine that peak 2 is sheep breast beta-casein and carries out dialysis postlyophilization to it.
From the above results, sheep breast beta-casein can be separated in vitro, eliminates the alpha-casein easily causing baby's Disorder of Digestion and A orption and allergy, constipation can not occur after baby drinks, be applicable to the baby of any physique.
Embodiment 3: the interpolation of desalted whey powder
In human milk simulating, the ratio of casein and lactalbumin, artificially adds desalted whey powder, makes the ratio of casein and lactalbumin in formula milk be 40:60.
Embodiment 4: preparation method
With the caseic addition of sheep breast β for 25g/100g, the addition of desalted whey powder is 37.5g/100g is example, preparation method, and concrete steps are as follows:
(1) from fresh milk, isolate bovine casein, obtain bovine casein and parting liquid, recycling rennin to be hydrolyzed modification to bovine casein;
(2) modified bovine casein, sheep breast beta-casein and desalted whey powder is added in the cow's milk parting liquid obtained to step (1), the caseic addition of described sheep breast β is 25g/100g, the addition of desalted whey powder is 37.5g/100g, and the ratio regulating casein and lactalbumin in formula milk is 40:60;
(3) utilize high pressure to carry out homogeneous the mixed liquor of step (2) gained, homogenization pressure is 16MPa, and homogenizing temperature is 45 DEG C;
(4) control sterilization temperature at 85 DEG C, sterilizing time is 24s;
(5) adopt pressure downflow type many shower nozzles drying tower to carry out spraying dry, obtain Powdered original washing powder;
(6) carbohydrate, dietary fiber, aliphatic acid, vitamin and the mineral matter Powdered original washing powder with step (5) gained is mixed, obtain baby formula milk powder;
(7) pack.
Embodiment 5: a kind of babies ' formula milk powder
A kind of baby formula milk powder, bovine casein is carried out modification by the basis of original baby formula, and add the desalted whey powder of sheep breast beta-casein and cow's milk, the content of casein and lactalbumin in human milk simulating, the ratio of both adjustments is 40:60.
A kind of formula of baby formula milk powder is: energy 2016kJ/100g, carbohydrate 26.1g/100g, dietary fiber 199mg/100g, linoleic acid 1925mg/100g, alpha-linolenic acid 304mg/100g, DHA 6867 μ g/100g, arachidonic acid 13.5mg/100g, vitamin A 469 μ g/100g, vitamin D 7.1 μ g/100g, vitamin E 4.82mg/100g, vitamin K
125.2 μ g/100g, Cobastab
1355 μ g/100g, Cobastab
2372 μ g/100g, Cobastab
6>=240 μ g/100g, Cobastab
12>=1.25 μ g/100g, vitamin C 45.8mg/100g, nicotinic acid 2785 μ g/100g, folic acid 30 μ g/100g, pantothenic acid 2050 μ g/100g, compound sugar 411mg/100g, lecithin 182mg/100g, nucleotides 15mg/100g, taurine 27mg/100g, inositol 39mg/100g, l-cn 8.6mg/100g, chlorine≤902mg/100g, biotin 14.0 μ g/100g, choline 43.1mg/100g, sodium≤381mg/100g, zinc 3.41mg/100g, selenium 12.0 μ g/100g, iodine 44 μ g/100g, copper 245 μ g/100g, manganese 40 ~ 44 μ g/100g, phosphorus 451mg/100g, potassium 461mg/100g, magnesium 40.7mg/100g, calcium 561mg/100g, iron 7.42mg/100g, with the addition of again sheep breast beta-casein 20g/100g, desalted whey powder 30g/100g, all the other are modified milk solids and fat.
Embodiment 6
A kind of formula of baby formula milk powder is: energy 2016kJ/100g, carbohydrate 26.1g/100g, dietary fiber 199mg/100g, linoleic acid 1925mg/100g, alpha-linolenic acid 304mg/100g, DHA 6867 μ g/100g, arachidonic acid 13.5mg/100g, vitamin A 469 μ g/100g, vitamin D 7.1 μ g/100g, vitamin E 4.82mg/100g, vitamin K
125.2 μ g/100g, Cobastab
1355 μ g/100g, Cobastab
2372 μ g/100g, Cobastab
6>=240 μ g/100g, Cobastab
12>=1.25 μ g/100g, vitamin C 45.8mg/100g, nicotinic acid 2785 μ g/100g, folic acid 30 μ g/100g, pantothenic acid 2050 μ g/100g, compound sugar 411mg/100g, lecithin 182mg/100g, nucleotides 15mg/100g, taurine 27mg/100g, inositol 39mg/100g, l-cn 8.6mg/100g, chlorine≤902mg/100g, biotin 14.0 μ g/100g, choline 43.1mg/100g, sodium≤381mg/100g, zinc 3.41mg/100g, selenium 12.0 μ g/100g, iodine 44 μ g/100g, copper 245 μ g/100g, manganese 40 ~ 44 μ g/100g, phosphorus 451mg/100g, potassium 461mg/100g, magnesium 40.7mg/100g, calcium 561mg/100g, iron 7.42mg/100g, with the addition of again sheep breast beta-casein 22.5g/100g, desalted whey 33.75g/100g all the other be modified milk solids and fat.
Embodiment 7
A kind of formula of baby formula milk powder is: energy 2016kJ/100g, carbohydrate 26.1g/100g, dietary fiber 199mg/100g, linoleic acid 1925mg/100g, alpha-linolenic acid 304mg/100g, DHA 6867 μ g/100g, arachidonic acid 13.5mg/100g, vitamin A 469 μ g/100g, vitamin D 7.1 μ g/100g, vitamin E 4.82mg/100g, vitamin K
125.2 μ g/100g, Cobastab
1355 μ g/100g, Cobastab
2372 μ g/100g, Cobastab
6>=240 μ g/100g, Cobastab
12>=1.25 μ g/100g, vitamin C 45.8mg/100g, nicotinic acid 2785 μ g/100g, folic acid 30 μ g/100g, pantothenic acid 2050 μ g/100g, compound sugar 411mg/100g, lecithin 182mg/100g, nucleotides 15mg/100g, taurine 27mg/100g, inositol 39mg/100g, l-cn 8.6mg/100g, chlorine≤902mg/100g, biotin 14.0 μ g/100g, choline 43.1mg/100g, sodium≤381mg/100g, zinc 3.41mg/100g, selenium 12.0 μ g/100g, iodine 44 μ g/100g, copper 245 μ g/100g, manganese 40 ~ 44 μ g/100g, phosphorus 451mg/100g, potassium 461mg/100g, magnesium 40.7mg/100g, calcium 561mg/100g, iron 7.42mg/100g, with the addition of again sheep breast beta-casein 25g/100g, desalted whey 37.5g/100g all the other be modified milk solids and fat.
Embodiment 8
A kind of formula of baby formula milk powder is: energy 2016kJ/100g, carbohydrate 26.1g/100g, dietary fiber 199mg/100g, linoleic acid 1925mg/100g, alpha-linolenic acid 304mg/100g, DHA 6867 μ g/100g, arachidonic acid 13.5mg/100g, vitamin A 469 μ g/100g, vitamin D 7.1 μ g/100g, vitamin E 4.82mg/100g, vitamin K
125.2 μ g/100g, Cobastab
1355 μ g/100g, Cobastab
2372 μ g/100g, Cobastab
6>=240 μ g/100g, Cobastab
12>=1.25 μ g/100g, vitamin C 45.8mg/100g, nicotinic acid 2785 μ g/100g, folic acid 30 μ g/100g, pantothenic acid 2050 μ g/100g, compound sugar 411mg/100g, lecithin 182mg/100g, nucleotides 15mg/100g, taurine 27mg/100g, inositol 39mg/100g, l-cn 8.6mg/100g, chlorine≤902mg/100g, biotin 14.0 μ g/100g, choline 43.1mg/100g, sodium≤381mg/100g, zinc 3.41mg/100g, selenium 12.0 μ g/100g, iodine 44 μ g/100g, copper 245 μ g/100g, manganese 40 ~ 44 μ g/100g, phosphorus 451mg/100g, potassium 461mg/100g, magnesium 40.7mg/100g, calcium 561mg/100g, iron 7.42mg/100g, with the addition of again sheep breast beta-casein 27.5g/100g, desalted whey powder 41.25g/100g, all the other are modified milk solids and fat.
Embodiment 9
A kind of formula of baby formula milk powder is: energy 2016kJ/100g, carbohydrate 26.1g/100g, dietary fiber 199mg/100g, linoleic acid 1925mg/100g, alpha-linolenic acid 304mg/100g, DHA 6867 μ g/100g, arachidonic acid 13.5mg/100g, vitamin A 469 μ g/100g, vitamin D 7.1 μ g/100g, vitamin E 4.82mg/100g, vitamin K
125.2 μ g/100g, Cobastab
1355 μ g/100g, Cobastab
2372 μ g/100g, Cobastab
6>=240 μ g/100g, Cobastab
12>=1.25 μ g/100g, vitamin C 45.8mg/100g, nicotinic acid 2785 μ g/100g, folic acid 30 μ g/100g, pantothenic acid 2050 μ g/100g, compound sugar 411mg/100g, lecithin 182mg/100g, nucleotides 15mg/100g, taurine 27mg/100g, inositol 39mg/100g, l-cn 8.6mg/100g, chlorine≤902mg/100g, biotin 14.0 μ g/100g, choline 43.1mg/100g, sodium≤381mg/100g, zinc 3.41mg/100g, selenium 12.0 μ g/100g, iodine 44 μ g/100g, copper 245 μ g/100g, manganese 40 ~ 44 μ g/100g, phosphorus 451mg/100g, potassium 461mg/100g, magnesium 40.7mg/100g, calcium 561mg/100g, iron 7.42mg/100g, with the addition of again sheep breast beta-casein 30g/100g, desalted whey powder 45g/100g, all the other are modified milk solids and fat.
Embodiment 10 new formulation milk powder improves immunity experiment
Get the small white mouse (entirely female) in 45 ~ 60 day age, be divided into control group, test group 1, test 2,3 groups, often organize 10, totally 30, only gavage physiological saline, ordinary powdered milk, embodiment 6 Novel baby formula milk (concentration is 1g/ml) 4mL/ respectively, once a day, continuous use 10d, then puts to death small white mouse by control group experimental group 1 experimental group 2 vertebra dislocation method, weigh in, take out spleen and weigh.Using the ratio of spleen weight/mg and body weight/g as index and spleen index.The impact of different preparations on mouse spleen index is as shown in table 1.
The different milk powder of table 1 is on the impact of mouse spleen index
As shown in table 1, find through data analysis, the index and spleen index difference between control group and test 2 groups extremely significantly (p<0.01); Index and spleen index between control group and test group 1 has significant difference (p<0.05).
From above experiment, receive with new formulation milk powder add common babies ' formula milk powder in feed after, all can significantly improve the index and spleen index of mouse, but after adding new formulation milk powder, index and spleen index increase is more, illustrates that the more common babies ' formula milk powder of this new formulation milk powder more can improve immunity.
Embodiment 11 new formulation milk powder antifatigue is tested
Swimming test is by small white mouse according to table 1 continuously gavage 10 days, and 60min after last gavage, weighs in respectively.Then the weight of 2.0g is lain in small white mouse root of the tail portion, then small white mouse is placed in the pond went swimming of 45cm × 30cm × 20cm, water temperature 18.5 DEG C, start timing.Be muscle power exhaustion when small white mouse head submerged 10s can not emerge, timing at once, be small white mouse swimming time, the impact of different preparation on mouse swimming time is as shown in table 2.
The different milk powder of table 2 is on the impact of mouse swimming time
Above description of test, the more common babies ' formula milk powder of this Novel baby formula milk is significantly improved in antifatigue.
Embodiment 12 new formulation milk powder promotes skeleton development experiment
Choose after 3 week age, cleaning grade pure lines male SD rat normal diet fed 4 weeks, fill with by force and feed 0.8g/d, gavage 10 days.Control group is the experimental group of gavage physiological saline, and experimental group 1 and experimental group 2 are respectively the experimental group of gavage regular convention formula milk powder and Novel baby formula milk.Gavage the 6th day, fluorescence matrix-tetracycline is injected in abdominal cavity, within 8th day, carry out the 2nd injection of fluorescence matrix-tetracycline, within 10th day, anesthetized rat is lethal, completely separate shin bone, by the distance between vernier caliper measurement proximal tibia articular surface and distal joint face, be the tibia length of feeding 10d, get the mean value measured for its 3 times, result as shown in Figure 4.It is 65 μm that Fig. 4 shows control group 0 tibia growth length, and experimental group 1 tibia growth length is 75 μm, and experimental group 2 tibia growth length is 82 μm, and the performance in promotion skeleton development of Novel baby formula milk is remarkable.
Claims (4)
1. a preparation method for cow-goat milk mixed-type infant formula milk powder, is characterized in that, method is as follows:
(1) from fresh milk, isolate bovine casein, obtain bovine casein and parting liquid, recycling rennin to be hydrolyzed modification to bovine casein;
(2) modified bovine casein, sheep breast beta-casein and desalted whey powder is added in the cow's milk parting liquid obtained to step (1), the caseic addition of described sheep breast β is 20g/100g ~ 30g/100g, the addition of desalted whey powder is 30g/100g ~ 45g/100g, and the ratio regulating casein and lactalbumin in formula milk is 40:60;
(3) utilize high pressure to carry out homogeneous the mixed liquor of step (2) gained, homogenization pressure is 15-20MPa, and homogenizing temperature is 40-50 DEG C;
(4) control sterilization temperature at 82-90 DEG C, sterilizing time is 20-25s;
(5) adopt pressure downflow type many shower nozzles drying tower to carry out spraying dry, obtain Powdered original washing powder;
(6) the Powdered original washing powder of preparing burden with step (5) gained is mixed, obtain baby formula milk powder;
Described batching is: energy 2016kJ/100g, carbohydrate 26.1g/100g, dietary fiber 199mg/100g, linoleic acid 1925mg/100g, alpha-linolenic acid 304mg/100g, DHA 6867 μ g/100g, arachidonic acid 13.5mg/100g, vitamin A 469 μ g/100g, vitamin D 7.1 μ g/100g, vitamin E 4.82mg/100g, vitamin K
125.2 μ g/100g, Cobastab
1355 μ g/100g, Cobastab
2372 μ g/100g, Cobastab
6>=240 μ g/100g, Cobastab
12>=1.25 μ g/100g, vitamin C 45.8mg/100g, nicotinic acid 2785 μ g/100g, folic acid 30 μ g/100g, pantothenic acid 2050 μ g/100g, compound sugar 411mg/100g, lecithin 182mg/100g, nucleotides 15mg/100g, taurine 27mg/100g, inositol 39mg/100g, l-cn 8.6mg/100g, chlorine≤902mg/100g, biotin 14.0 μ g/100g, choline 43.1mg/100g, sodium≤381mg/100g, zinc 3.41mg/100g, selenium 12.0 μ g/100g, iodine 44 μ g/100g, copper 245 μ g/100g, manganese 40 ~ 44 μ g/100g, phosphorus 451mg/100g, potassium 461mg/100g, magnesium 40.7mg/100g, calcium 561mg/100g, iron 7.42mg/100g, all the other are cow's milk solid content and fat,
(7) pack.
2. preparation method according to claim 1, is characterized in that, the rennin described in step (1) is calf rennin.
3. preparation method according to claim 1, is characterized in that, the caseic addition of described sheep breast β is 25g/100g, and the addition of desalted whey powder is 37.5g/100g.
4. preparation method according to claim 1, is characterized in that, concrete steps are as follows:
(1) bovine casein modification: isolate bovine casein from fresh milk, obtains bovine casein and parting liquid, and recycling rennin to be hydrolyzed modification to casein;
(2) protein ratio is adjusted: in the cow's milk parting liquid that step (1) obtains, add modified bovine casein, sheep breast beta-casein and desalted whey powder, the caseic addition of described sheep breast β is 25g/100g, the addition of desalted whey powder is 37.5g/100g, and the ratio regulating casein and lactalbumin in formula milk is 40:60;
(3) homogeneous: utilize high pressure to carry out homogeneous the mixed liquor of step (2) gained, homogenization pressure is 16MPa, and homogenizing temperature is 45 DEG C;
(4) sterilization: control sterilization temperature at 85 DEG C, sterilizing time is 24s;
(5) spraying dry: adopt pressure downflow type many shower nozzles drying tower to carry out spraying dry, obtain Powdered original washing powder;
(6) be dry mixed: the Powdered original washing powder of preparing burden with step (5) gained is mixed, obtain baby formula milk powder;
Described batching is: energy 2016kJ/100g, carbohydrate 26.1g/100g, dietary fiber 199mg/100g, linoleic acid 1925mg/100g, alpha-linolenic acid 304mg/100g, DHA 6867 μ g/100g, arachidonic acid 13.5mg/100g, vitamin A 469 μ g/100g, vitamin D 7.1 μ g/100g, vitamin E 4.82mg/100g, vitamin K
125.2 μ g/100g, Cobastab
1355 μ g/100g, Cobastab
2372 μ g/100g, Cobastab
6>=240 μ g/100g, Cobastab
12>=1.25 μ g/100g, vitamin C 45.8mg/100g, nicotinic acid 2785 μ g/100g, folic acid 30 μ g/100g, pantothenic acid 2050 μ g/100g, compound sugar 411mg/100g, lecithin 182mg/100g, nucleotides 15mg/100g, taurine 27mg/100g, inositol 39mg/100g, l-cn 8.6mg/100g, chlorine≤902mg/100g, biotin 14.0 μ g/100g, choline 43.1mg/100g, sodium≤381mg/100g, zinc 3.41mg/100g, selenium 12.0 μ g/100g, iodine 44 μ g/100g, copper 245 μ g/100g, manganese 40 ~ 44 μ g/100g, phosphorus 451mg/100g, potassium 461mg/100g, magnesium 40.7mg/100g, calcium 561mg/100g, iron 7.42mg/100g, all the other are cow's milk solid content and fat,
(7) pack: after formula milk exhaust thoroughly, tightly seal.
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| CN105053204A (en) * | 2015-07-24 | 2015-11-18 | 林晓萍 | Administration method of premature infant milk powder for promoting bone growth and development of premature infant |
| CN105076425A (en) * | 2015-09-17 | 2015-11-25 | 许德建 | Yak, cow and ewe three-in-one composite infant formula milk powder |
| CN107397230A (en) * | 2017-08-21 | 2017-11-28 | 杭州特悘衡康生物科技有限公司 | Alimentation composition, the formula food for promoting Post operation muscle recovery and protein synthesis and its application |
| CN111989570A (en) * | 2018-04-19 | 2020-11-24 | 埃利有限责任公司 | Sample clarification and background fluorescence reduction for analyte fluorescence detection |
| WO2021003741A1 (en) * | 2019-07-11 | 2021-01-14 | 北京三元食品股份有限公司 | APPLICATION OF β-CASEIN A2 AND COMPOSITION THEREOF IN PROMOTING PROLIFERATION OF BIFIDOBACTERIUM |
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| CN1656906A (en) * | 2005-03-16 | 2005-08-24 | 石家庄三鹿集团股份有限公司 | Formulated milk powder for children and its preparation method |
| CN102283289A (en) * | 2011-09-15 | 2011-12-21 | 澳优乳业（中国）有限公司 | Anti-inflaming infant formula milk powder and preparation process thereof |
| CN102763727A (en) * | 2012-07-02 | 2012-11-07 | 李晓林 | Dairy cattle and goat milk composite infant formula milk powder |
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| CN1656906A (en) * | 2005-03-16 | 2005-08-24 | 石家庄三鹿集团股份有限公司 | Formulated milk powder for children and its preparation method |
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