CN103623370A - Medicine for treating pseudodactylogrus anguillae parasitism and preparation method thereof - Google Patents
Medicine for treating pseudodactylogrus anguillae parasitism and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a medicine for treating pseudodactylogrus anguillae parasitism and a preparation method thereof. The medicine is prepared from the following medicinal materials: 10-30 parts of root of Chinese pulsatilla, 3-9 parts of asarum, 6-15 parts of cassia twig, 3-12 parts of radix sophorae flavescentis, 1-10 parts of processed radix aconiti lateralis, 10-30 parts of dried ginger, 3-12 parts of pericarpium zanthoxyli, 5-15 parts of rheum officinale, 10-30 parts of areca-nut, 10-30 parts of amargosa bark, 5-25 parts of edible tulip, 20-50 parts of quispualis indica and 10-30 parts of fructus cnidii. As the Chinese pulsatilla has the effects of clearing away heat and toxic materials, cooling blood, stopping dysentery, removing dampness and destroying parasites, the asarum has the effects of activating blood circulation, eliminating toxicants and relieving pain, and the cassia twig has the effects of supporting yang to transform qi, calming qi uprush and depressing qi, the medicine is capable of effectively eliminating hyperaemia and edema of eel gill filament, mucus increasing and decreasing and gill lamella cell proliferation which are caused by parasitism pseudodactylogyrus, and preventing the swelling and bleeding of liver and gall which is caused by secondary bacterial infection. The medicine has the characteristics of being reasonable in formula, safe, non-toxic and stable in curative effect, quickly taking effect and the like.
Description
Technical field
The present invention relates to a kind of fisheries drug, particularly relate to a kind of treat genus Pseudodactylogrus from Anguilla anguilla parasitism cause the congested edema of the anguilla japonica gill filament, mucus increase come off, the hyperplasia of gill small pieces, abnormal and dyspnea move about, the infection of Secondary cases antibacterial, loss of appetite, sting food or go on a hunger strike, and then causing anguilla japonica genus Pseudodactylogrus from Anguilla anguilla parasitic disease medicine of extensive fulminant disease and preparation method thereof.
Background technology
Genus Pseudodactylogrus from Anguilla anguilla is monogentic trematode class parasite the most common in anguilla japonica cultivation, that Epidemic Scope is wide, harm is larger, main parasitic is in the gill portion of European eel, Japanese eel and eel, anniversary occurs, on the gill, move, adhere to, and the chitin hook on holdfast inserts gill tissue thereafter, destroy the epidermis cell of the gill, stimulate the mucous secretion of gill epithelial cell to increase, impact is breathed, cause gill filament hyperemia and Secondary cases antibacterial to infect, especially in anguilla japonica high-density breeding situation, very easily cause large-scale fulminant disease.Ill anguilla japonica moves about extremely, dyspnea, loss of appetite, sting food or go on a hunger strike, once morbidity, easily repeated infection, causes mortality, and economic loss is heavy.
In anguilla japonica genus Pseudodactylogrus from Anguilla anguilla parasitic disease control, adopted organic phosphates can effectively drive away anguilla japonica genus Pseudodactylogrus from Anguilla anguilla as praziquantel etc. as mebendazole, zionoes as metrifonate, imidazoles in the past.But along with the life-time service of similar drugs, cause genus Pseudodactylogrus from Anguilla anguilla such medicine to be produced to stronger drug resistance, even if improve Drug level, be also difficult to drive away.Eel field is in order to reduce economic loss, and Drug abuse or continue to increase dosage causes water environment severe contamination, drug residue to exceed standard, and forms vicious cycle.And strictly limit in the world the chemicals residual quantity in anguilla japonica product at present, cause the present situation that in anguilla japonica breeding production, appearance can be used without medicine, once genus Pseudodactylogrus from Anguilla anguilla is parasitic in a large number, grievous injury anguilla japonica gill tissue, and then pathogen invasion, will cause anguilla japonica mortality, economic loss is huge.Therefore find the novel Aquatic product insecticide of killing genus Pseudodactylogrus from Anguilla anguilla, seem and be even more important.The natural botanical insecticide with advantages such as low toxicity, safety, environmental protections is best selections, but very few at Aquatic product area research report at present.On current fisheries drug market, have not yet to see the safe drugs of low residual, the environmental protection of efficient, the low toxicity of killing anguilla japonica genus Pseudodactylogrus from Anguilla anguilla of having developed.
Summary of the invention
One object of the present invention is to provide a kind of medicinal efficacy to bring into play the compound medicine of killing anguilla japonica genus Pseudodactylogrus from Anguilla anguilla abundant, rapid-action, low toxicity.
Another is to provide the preparation method that a kind of preparation manipulation is simple, easily go, be conducive to the anguilla japonica genus Pseudodactylogrus from Anguilla anguilla parasitic disease medicine of large-scale industrialization production object of the present invention.
For achieving the above object, technical solution of the present invention is:
The present invention is a kind of anguilla japonica genus Pseudodactylogrus from Anguilla anguilla parasitic disease medicine, and it comprises the crude drug of following weight portion: 10 parts～30 parts of the Radix Pulsatillaes, 3 parts～9 parts of Herba Asaris, 6 parts～15 parts of Ramulus Cinnamomi, 3 parts～12 parts of Radix Sophorae Flavescentiss, 1 part～10 parts of Radix Aconiti Lateralis Preparata, 10 parts～30 parts of Rhizoma Zingiberiss, 3 parts～12 parts of Fructus Zanthoxylis, 5 parts～15 parts of Radix Et Rhizoma Rhei, 10 parts～30 parts of Semen Arecaes, 10 parts～30 parts of melias, 5 parts～25 parts of Pseudobulbus cremastrae seu pleiones, 20 parts～50 parts of Fructus Quisqualis, 10 parts～30 parts of Fructus Cnidiis.
Optimization formula scope is: 10 parts～30 parts of the Radix Pulsatillaes, 3 parts～6 parts of Herba Asaris, 6 parts～10 parts of Ramulus Cinnamomi, 6 parts～9 parts of Radix Sophorae Flavescentiss, 3 parts～6 parts of Radix Aconiti Lateralis Preparata, 15 parts～25 parts of Rhizoma Zingiberiss, 5 parts～10 parts of Fructus Zanthoxylis, 6 parts～10 parts of Radix Et Rhizoma Rhei, 15 parts～25 parts of Semen Arecaes, 15 parts～20 parts of melias, 10 parts～20 parts of Pseudobulbus cremastrae seu pleiones, 30 parts～50 parts of Fructus Quisqualis, 15 parts～30 parts of Fructus Cnidiis.
Optimization formula is: the Radix Pulsatillae 20, Herba Asari 6, Ramulus Cinnamomi 6, Radix Sophorae Flavescentis 6, Radix Aconiti Lateralis Preparata 3, Rhizoma Zingiberis 12, Fructus Zanthoxyli 6, Radix Et Rhizoma Rhei 10, Semen Arecae 15, melia 15, Pseudobulbus cremastrae seu pleiones 10, Fructus Quisqualis 30,15 parts of Fructus Cnidiis.
The present invention is a kind of preparation method of anguilla japonica genus Pseudodactylogrus from Anguilla anguilla parasitic disease medicine, and it comprises the following steps:
(1) according to amount ratio, the Radix Pulsatillae, Radix Et Rhizoma Rhei, Radix Sophorae Flavescentis are added to 10 ~ 15 times of amount microwaves of water lixiviate twice, be heated to maintain 30 minutes after boiling standing, filtration at every turn, collecting decoction, it is standby that microwave vacuum is concentrated into the clear paste of Baume degrees 1.25～1.28 of 60 ℃ of relative densities;
(2) according to amount ratio, by Herba Asari, Ramulus Cinnamomi, Radix Aconiti Lateralis Preparata, Rhizoma Zingiberis, Fructus Zanthoxyli, Semen Arecae, melia, Pseudobulbus cremastrae seu pleiones, Fructus Quisqualis, Fructus Cnidii, with 10 ~ 15 times of amount reflux, extract, secondaries of percentage by weight 65% ethanol, each microwave heating maintains 30 minutes to boiling, standing, filtration, merge extractive liquid,, reclaims ethanol, and it is standby that filtrate microwave vacuum is concentrated into the clear paste of Baume degrees 1.25～1.28 of 60 ℃ of relative densities;
(3) medicinal residues in step (1) and (2) are added again to 10 ~ 15 times of amount microwave extraction secondaries of 65% ethanol water, be heated to maintain 30 minutes after boiling at every turn, standing, filtration, merge extractive liquid,, reclaim ethanol, it is standby that filtrate is concentrated into the clear paste of Baume degrees 1.25～1.28 of 60 ℃ of relative densities;
(4) three kinds of clear paste of combining step (1), (2) and (3), being concentrated into relative density is the thick paste of the Baume degrees 1.35～1.38 of 60 ℃, microwave vacuum drying, freezing and pulverizing becomes fine powder, is prepared into the pharmaceutical composition for the treatment of anguilla japonica genus Pseudodactylogrus from Anguilla anguilla parasitic disease.
The present invention is a kind of preparation method of anguilla japonica genus Pseudodactylogrus from Anguilla anguilla parasitic disease medicine, and it comprises the following steps:
(1) according to amount ratio, by Ramulus Cinnamomi, Fructus Quisqualis, adopt freezing and micronizing machine to pulverize, be crushed to superfine powder more than 300 orders, standby;
(2) according to amount ratio, by the Radix Pulsatillae, Radix Et Rhizoma Rhei, Radix Sophorae Flavescentis, Herba Asari, Radix Aconiti Lateralis Preparata, Rhizoma Zingiberis, Fructus Zanthoxyli, Semen Arecae, melia, Pseudobulbus cremastrae seu pleiones, Fructus Cnidii, with 10 ~ 15 times of amount reflux, extract, secondaries of percentage by weight 65% ethanol, each microwave heating maintains 30 minutes to boiling, standing, filtration, merge extractive liquid,, reclaims ethanol, and it is standby that filtrate is concentrated into the clear paste of Baume degrees 1.25～1.28 of 60 ℃ of relative densities;
(3) medicinal residues of step (2) are added to 10 ~ 15 times, water amount microwave extraction secondaries again, be heated to maintain 30 minutes after boiling at every turn, standing, filter, collecting decoction, it is standby that microwave vacuum is concentrated into the clear paste of Baume degrees 1.25～1.28 of 60 ℃ of relative densities;
(4) two kinds of clear paste of the superfine powder of combining step (1), step (2) and (3), through microwave vacuum drying, freezing and pulverizing becomes fine powder, is prepared into the pharmaceutical composition for the treatment of anguilla japonica genus Pseudodactylogrus from Anguilla anguilla parasitic disease.
Adopt after such scheme, the present invention has the following advantages:
(1) the present invention be take tcm theory as basis, configures scientifically and rationally each component drug and consumption in raw material of Chinese medicine, and the drug effect of each component can organically be combined, and comprehensively brings into play medicinal effects, and the therapeutic effect of medicine is good, and toxicity is little.We get heat-clearing and toxic substances removing, eliminating pathogenic heat from blood to cure dysentery, the removing dampness and killing parasites of the Radix Pulsatillae; The promoting blood circulation and detoxication of Herba Asari, pain relieving; The supporing yang activating QI of Ramulus Cinnamomi, the flat gas that spins; The heat clearing and damp drying of Radix Sophorae Flavescentis, parasite killing; Radix Aconiti Lateralis Preparata and Rhizoma Zingiberis be with can recuperating depleted YANG and rescuing the patient from collapse, can on help heart-yang, middle warming the spleen sun, lower kidney-replenishing; The warming middle-JIAO of Fructus Zanthoxyli, pain relieving, parasite killing; Radix Et Rhizoma Rhei purging heat and dredging bowels, removing pathogenic heat from blood and toxic substance from the body, removing blood stasis tissue regeneration promoting is strengthened renal metabolism; The expelling intestinal worms and removing food stagnancy of Semen Arecae, promoting the circulation of QI to induce diuresis; Melia has the effect of parasite killing; The detumescence of Pseudobulbus cremastrae seu pleiones, reduce phlegm, detoxify; The anthelmintic of Fructus Quisqualis, hold up taste; The kidney warming of Fructus Cnidii, removing dampness and killing parasites.
(2) pharmaceutical composition of the present invention can effectively eliminate because genus Pseudodactylogrus from Anguilla anguilla parasitizes anguilla japonica gill portion cause the congested edema of the gill filament, mucus to increase coming off, the hyperplasia of gill small pieces, prevent that secondary antibacterial from infecting and liver and gall enlargement, the disease such as hemorrhage, there is formula rationally, rapid-action (the onset time 5-7 days that anti-gill filament unsticking is hemorrhage, cure required time 23 days), the features such as safety non-toxic, stable curative effect.Through cultivation site medication, observe, sick eelfare is moving and breathe and recover normal, the improving situation of ingesting, food ration obviously increases, do not occur antibacterial infect dead, good effect and having no side effect.At 4-9 month morbidity season, mix in feedstuff and feed, can prevent the outburst of genus Pseudodactylogrus from Anguilla anguilla disease.
(3) pharmaceutical composition of the present invention combines microwave lixiviate in preparation process with freezing and micronizing, the effective ingredient of pharmaceutical composition is not destroyed, content is high, and through freezing and micronizing, the effective ingredient of medicine is more conducive to fish body and absorbs, farthest bring into play drug effect, and preparation manipulation is easily capable, is conducive to large-scale industrialization and produces.
Below in conjunction with specific embodiment, the present invention is further illustrated.
The specific embodiment
one, formula:
The present invention is a kind of anguilla japonica genus Pseudodactylogrus from Anguilla anguilla parasitic disease medicine, and it comprises the crude drug of following weight portion: 10 parts～30 parts of the Radix Pulsatillaes, 3 parts～9 parts of Herba Asaris, 6 parts～15 parts of Ramulus Cinnamomi, 3 parts～12 parts of Radix Sophorae Flavescentiss, 1 part～10 parts of Radix Aconiti Lateralis Preparata, 10 parts～30 parts of Rhizoma Zingiberiss, 3 parts～12 parts of Fructus Zanthoxylis, 5 parts～15 parts of Radix Et Rhizoma Rhei, 10 parts～30 parts of Semen Arecaes, 10 parts～30 parts of melias, 5 parts～25 parts of Pseudobulbus cremastrae seu pleiones, 20 parts～50 parts of Fructus Quisqualis, 10 parts～30 parts of Fructus Cnidiis.
embodiment 1
Optimum formula: it is according to parts by weight, the Radix Pulsatillae 20, Herba Asari 6, Ramulus Cinnamomi 6, Radix Sophorae Flavescentis 6, Radix Aconiti Lateralis Preparata 3, Rhizoma Zingiberis 12, Fructus Zanthoxyli 6, Radix Et Rhizoma Rhei 10, Semen Arecae 15, melia 15, Pseudobulbus cremastrae seu pleiones 10, Fructus Quisqualis 30,15 parts of Fructus Cnidiis.
embodiment 2
It is according to parts by weight, the Radix Pulsatillae 20, Herba Asari 3, Ramulus Cinnamomi 9, Radix Sophorae Flavescentis 9, Radix Aconiti Lateralis Preparata 5, Rhizoma Zingiberis 10, Fructus Zanthoxyli 6, Radix Et Rhizoma Rhei 5, Semen Arecae 20, melia 10, Pseudobulbus cremastrae seu pleiones 5, Fructus Quisqualis 30,20 parts of Fructus Cnidiis.
embodiment 3
It is according to parts by weight, the Radix Pulsatillae 15, Herba Asari 6, Ramulus Cinnamomi 15, Radix Sophorae Flavescentis 12, Radix Aconiti Lateralis Preparata 5, Rhizoma Zingiberis 15, Fructus Zanthoxyli 9, Radix Et Rhizoma Rhei 5, Semen Arecae 30, melia 20, Pseudobulbus cremastrae seu pleiones 5, Fructus Quisqualis 25,25 parts of Fructus Cnidiis.
embodiment 4
It is according to parts by weight, the Radix Pulsatillae 10, Herba Asari 9, Ramulus Cinnamomi 15, Radix Sophorae Flavescentis 9, Radix Aconiti Lateralis Preparata 10, Rhizoma Zingiberis 20, Fructus Zanthoxyli 9, Radix Et Rhizoma Rhei 10, Semen Arecae 25, melia 25, Pseudobulbus cremastrae seu pleiones 10, Fructus Quisqualis 50,20 parts of Fructus Cnidiis.
embodiment 5
It is according to parts by weight, the Radix Pulsatillae 25, Herba Asari 6, Ramulus Cinnamomi 12, Radix Sophorae Flavescentis 3, Radix Aconiti Lateralis Preparata 5, Rhizoma Zingiberis 20, Fructus Zanthoxyli 12, Radix Et Rhizoma Rhei 15, Semen Arecae 10, melia 20, Pseudobulbus cremastrae seu pleiones 15, Fructus Quisqualis 30,15 parts of Fructus Cnidiis.
embodiment 6
It is according to parts by weight, the Radix Pulsatillae 30, Herba Asari 6, Ramulus Cinnamomi 9, Radix Sophorae Flavescentis 6, Radix Aconiti Lateralis Preparata 10, Rhizoma Zingiberis 15, Fructus Zanthoxyli 3, Radix Et Rhizoma Rhei 10, Semen Arecae 10, melia 15, Pseudobulbus cremastrae seu pleiones 20, Fructus Quisqualis 20,10 parts of Fructus Cnidiis.
embodiment 7
It is according to parts by weight, the Radix Pulsatillae 25, Herba Asari 3, Ramulus Cinnamomi 6, Radix Sophorae Flavescentis 3, Radix Aconiti Lateralis Preparata 1, Rhizoma Zingiberis 12, Fructus Zanthoxyli 6, Radix Et Rhizoma Rhei 5, Semen Arecae 15, melia 30, Pseudobulbus cremastrae seu pleiones 25, Fructus Quisqualis 25,15 parts of Fructus Cnidiis.
embodiment 8
It is according to parts by weight, the Radix Pulsatillae 10, Herba Asari 9, Ramulus Cinnamomi 12, Radix Sophorae Flavescentis 12, Radix Aconiti Lateralis Preparata 1, Rhizoma Zingiberis 30, Fructus Zanthoxyli 9, Radix Et Rhizoma Rhei 15, Semen Arecae 20, melia 30, Pseudobulbus cremastrae seu pleiones 25, Fructus Quisqualis 40,30 parts of Fructus Cnidiis.
embodiment 9
It is according to parts by weight, the Radix Pulsatillae 15, Herba Asari 3, Ramulus Cinnamomi 6, Radix Sophorae Flavescentis 6, Radix Aconiti Lateralis Preparata 3, Rhizoma Zingiberis 25, Fructus Zanthoxyli 12, Radix Et Rhizoma Rhei 15, Semen Arecae 30, melia 10, Pseudobulbus cremastrae seu pleiones 15, Fructus Quisqualis 25,25 parts of Fructus Cnidiis.
The traditional Chinese medicinal material raw materials that the present invention is used:
The Radix Pulsatillae: this product is the dry root of ranunculaceae plant Radix Pulsatillae Pulsatilla chinensis (Bge.) Regel.
Herba Asari: this product is the dry herb of aristolochiaceae plant Herba Asari Asarum heterotropoides Fr. Schmidt var. mandshuricum (Maxim.) Kitag., Seoul Herba Asari Asarum sieboldii Miq. f.seoulense (Nak.) C.Y. Cheng et C.S. Yang or magnificent Herba Asari Asarum sieboldii Miq..
Ramulus Cinnamomi: this product is the dry twig of canella Cortex Cinnamomi Cinnamomum cassia Presl.
Radix Sophorae Flavescentis: this product is the dry root of leguminous plant Radix Sophorae Flavescentis Sophora flavescens Ait..
Radix Aconiti Lateralis Preparata: this product is the processed goods of the daughter root of ranunculaceae plant Aconitum carmichjaelii Debx. Aconitum carmichaeli Debx..
Rhizoma Zingiberis: this product is the dry rhizome of zingiber Zingiber officinale Rosc..
Fructus Zanthoxyli: this product is the dry mature fruit of Rutaceae Rutaceae Zanthoxylum Zanthoxylum.
Radix Et Rhizoma Rhei: this product is dry root and the rhizome of Polygonaceae Rheum Polygonaceae Rheu plant sorrel, Rheum tanguticum or Rheum officinale etc.
Semen Arecae: this product is the dry mature seed of babassu Semen Arecae Areca catechu L..
Melia: this product is bark and the root bark of Meliaceae plant chinaberry and Fructus Toosendan Melia azedarach L..
Pseudobulbus cremastrae seu pleiones: this product is the dry bulb of liliaceous plant Tulipa edulis and T.iliensis Tulipa iliensis Regel..
Fructus Quisqualis: this product is the dry mature fruit of Combretum Racemosum plant Fructus Quisqualis Quisqualis indica L..
Fructus Cnidii: this product is the dry mature fruit of samphire cnidium monnieri Cnidium monnieri (L.) Cuss..
two, preparation method:
embodiment 1:a kind of preparation method of anguilla japonica genus Pseudodactylogrus from Anguilla anguilla parasitic disease medicine
:
(1) according to amount ratio, the Radix Pulsatillae, Radix Et Rhizoma Rhei, Radix Sophorae Flavescentis are added to 10 ~ 15 times of amount microwaves of water lixiviate twice, be heated to maintain 30 minutes after boiling standing, filtration at every turn, collecting decoction, it is standby that microwave vacuum is concentrated into the clear paste of Baume degrees 1.25～1.28 of 60 ℃ of relative densities;
(2) by described amount ratio, by Herba Asari, Ramulus Cinnamomi, Radix Aconiti Lateralis Preparata, Rhizoma Zingiberis, Fructus Zanthoxyli, Semen Arecae, melia, Pseudobulbus cremastrae seu pleiones, Fructus Quisqualis, Fructus Cnidii, with 10 ~ 15 times of amount reflux, extract, secondaries of percentage by weight 65% ethanol, each microwave heating maintains 30 minutes to boiling, standing, filtration, merge extractive liquid,, reclaims ethanol, and it is standby that filtrate microwave vacuum is concentrated into the clear paste of Baume degrees 1.25～1.28 of 60 ℃ of relative densities;
(3) medicinal residues in step (1) and (2) are added again to 10 ~ 15 times of amount microwave extraction secondaries of 65% ethanol water, be heated to maintain 30 minutes after boiling at every turn, standing, filtration, merge extractive liquid,, reclaim ethanol, it is standby that filtrate is concentrated into the clear paste of Baume degrees 1.25～1.28 of 60 ℃ of relative densities;
(4) three kinds of clear paste of combining step (1), (2) and (3), being concentrated into relative density is the thick paste of the Baume degrees 1.35～1.38 of 60 ℃, microwave vacuum drying, freezing and pulverizing becomes fine powder, is prepared into the pharmaceutical composition for the treatment of anguilla japonica genus Pseudodactylogrus from Anguilla anguilla parasitic disease.
embodiment 2:a kind of preparation method of anguilla japonica genus Pseudodactylogrus from Anguilla anguilla parasitic disease medicine
:
(1) according to amount ratio, by Ramulus Cinnamomi, Fructus Quisqualis, adopt freezing and micronizing machine to pulverize, be crushed to superfine powder more than 300 orders, standby;
(2) by described amount ratio, by the Radix Pulsatillae, Radix Et Rhizoma Rhei, Radix Sophorae Flavescentis, Herba Asari, Radix Aconiti Lateralis Preparata, Rhizoma Zingiberis, Fructus Zanthoxyli, Semen Arecae, melia, Pseudobulbus cremastrae seu pleiones, Fructus Cnidii, with 10 ~ 15 times of amount reflux, extract, secondaries of percentage by weight 65% ethanol, each microwave heating maintains 30 minutes to boiling, standing, filtration, merge extractive liquid,, reclaims ethanol, and it is standby that filtrate is concentrated into the clear paste of Baume degrees 1.25～1.28 of 60 ℃ of relative densities;
(3) medicinal residues of step (2) are added to 10 ~ 15 times, water amount microwave extraction secondaries again, be heated to maintain 30 minutes after boiling at every turn, standing, filter, collecting decoction, it is standby that microwave vacuum is concentrated into the clear paste of Baume degrees 1.25～1.28 of 60 ℃ of relative densities;
(4) two kinds of clear paste of the superfine powder of combining step (1), step (2) and (3), through microwave vacuum drying, freezing and pulverizing becomes fine powder, is prepared into the pharmaceutical composition for the treatment of anguilla japonica genus Pseudodactylogrus from Anguilla anguilla parasitic disease.
three, the pharmaceutical composition test of pesticide effectiveness of the present invention
1, long term toxicity test
Test objective: observe healthy anguilla japonica long-term taking and treat toxic reaction and the degree that the pharmaceutical composition of genus Pseudodactylogrus from Anguilla anguilla parasitic disease occurs, for its drug safety of objective evaluation provides scientific basis.
Result of the test:
Give for a long time pharmaceutical composition of the present invention to anguilla japonica behavior outward appearance without obvious toxic effect.
Give for a long time pharmaceutical composition of the present invention, in test on the growth course of the heart of anguilla japonica, liver, spleen, lung, kidney without impact.
histopathology test
Histopathologic examination shows that in test, the parasitic quantity of anguilla japonica gill portion genus Pseudodactylogrus from Anguilla anguilla gradually reduces, and gill filament hyperemia is disappeared gradually, and it is normal that mucous secretion recovers, and impaired gill small pieces are repaired gradually, and the organs and tissues such as Liver and kidney gallbladder change without pathology.
Object: expelling worm, antiinflammatory, the detumescence effect of studying pharmaceutical composition of the present invention.
Method: select parasitic genus Pseudodactylogrus from Anguilla anguilla to cause moving about abnormal and respiratory frequency is accelerated, the sick eel of the symptoms such as mucus increases is raised and takes administration on gill filament hyperemia edema and the gill, studies expelling worm, antiinflammatory, the detumescence effect of pharmaceutical composition of the present invention.
Result: pharmaceutical composition of the present invention can significantly be dispelled gill portion genus Pseudodactylogrus from Anguilla anguilla, greatly reduces the destruction of genus Pseudodactylogrus from Anguilla anguilla zygobranchiate, and gill portion mucous secretion in 5-7 days recovers normal; Gill filament hyperemia in 15-17 days starts to disappear, and antibacterial infection does not occur and fester; 20-23 days severe infections persons' gill small pieces epithelial cell and myxocyte hypertrophy situation are repaired, and gill small pieces are obviously subsided a swelling; Immunoglobulin G level raises to some extent.
Conclusion: pharmaceutical composition of the present invention has obvious expelling worm, antiinflammatory, detumescence effect.
treatment efficiency assay
The pharmaceutical chemistry verification experimental verification of throwing something and feeding in breeding production, successive administration is observed the impact of pharmaceutical composition of the present invention on the sick eel of parasitic genus Pseudodactylogrus from Anguilla anguilla for 30 days afterwards.
Conclusion: to three kinds of anguilla japonicas pharmaceutical chemistry test group of throwing something and feeding, the moving about and breathe and recover gradually normal of sick eel, the situation of ingesting progressively takes a turn for the better, and every tail average weight is all significantly increased, and survival rate is also significantly higher than matched group.
To the curative effect total effective rate of eel, be 54.53%.
To the curative effect total effective rate of European eel, be 49.66%.
To the curative effect total effective rate of Japanese eel, be 65.38%.
The above, be only preferred embodiment of the present invention, it is multiple that the combining form of formula can have, therefore can not limit scope of the invention process with this, the equivalence of doing according to the present patent application the scope of the claims and description changes and modifies, and all should still remain within the scope of the patent.
Claims (5)
1. an anguilla japonica genus Pseudodactylogrus from Anguilla anguilla parasitic disease medicine, is characterized in that: it comprises the crude drug of following weight portion: 10 parts～30 parts of the Radix Pulsatillaes, 3 parts～9 parts of Herba Asaris, 6 parts～15 parts of Ramulus Cinnamomi, 3 parts～12 parts of Radix Sophorae Flavescentiss, 1 part～10 parts of Radix Aconiti Lateralis Preparata, 10 parts～30 parts of Rhizoma Zingiberiss, 3 parts～12 parts of Fructus Zanthoxylis, 5 parts～15 parts of Radix Et Rhizoma Rhei, 10 parts～30 parts of Semen Arecaes, 10 parts～30 parts of melias, 5 parts～25 parts of Pseudobulbus cremastrae seu pleiones, 20 parts～50 parts of Fructus Quisqualis, 10 parts～30 parts of Fructus Cnidiis.
2. anguilla japonica genus Pseudodactylogrus from Anguilla anguilla parasitic disease medicine according to claim 1, is characterized in that: it comprises the crude drug of following weight portion: 10 parts～30 parts of the Radix Pulsatillaes, 3 parts～6 parts of Herba Asaris, 6 parts～10 parts of Ramulus Cinnamomi, 6 parts～9 parts of Radix Sophorae Flavescentiss, 3 parts～6 parts of Radix Aconiti Lateralis Preparata, 15 parts～25 parts of Rhizoma Zingiberiss, 5 parts～10 parts of Fructus Zanthoxylis, 6 parts～10 parts of Radix Et Rhizoma Rhei, 15 parts～25 parts of Semen Arecaes, 15 parts～20 parts of melias, 10 parts～20 parts of Pseudobulbus cremastrae seu pleiones, 30 parts～50 parts of Fructus Quisqualis, 15 parts～30 parts of Fructus Cnidiis.
3. anguilla japonica genus Pseudodactylogrus from Anguilla anguilla parasitic disease medicine according to claim 1 and 2, is characterized in that: it comprises the crude drug of following weight portion: the Radix Pulsatillae 30, Herba Asari 6, Ramulus Cinnamomi 6, Radix Sophorae Flavescentis 6, Radix Aconiti Lateralis Preparata 3, Rhizoma Zingiberis 12, Fructus Zanthoxyli 6, Radix Et Rhizoma Rhei 10, Semen Arecae 15, melia 15, Pseudobulbus cremastrae seu pleiones 10, Fructus Quisqualis 30,15 parts of Fructus Cnidiis.
4. a preparation method for anguilla japonica genus Pseudodactylogrus from Anguilla anguilla parasitic disease medicine according to claim 1, is characterized in that: it comprises the following steps:
(1) according to amount ratio, the Radix Pulsatillae, Radix Et Rhizoma Rhei, Radix Sophorae Flavescentis are added to 10 ~ 15 times of amount microwaves of water lixiviate twice, be heated to maintain 30 minutes after boiling standing, filtration at every turn, collecting decoction, it is standby that microwave vacuum is concentrated into the clear paste of Baume degrees 1.25～1.28 of 60 ℃ of relative densities;
(2) according to amount ratio, by Herba Asari, Ramulus Cinnamomi, Radix Aconiti Lateralis Preparata, Rhizoma Zingiberis, Fructus Zanthoxyli, Semen Arecae, melia, Pseudobulbus cremastrae seu pleiones, Fructus Quisqualis, Fructus Cnidii, with 10 ~ 15 times of amount reflux, extract, secondaries of percentage by weight 65% ethanol, each microwave heating maintains 30 minutes to boiling, standing, filtration, merge extractive liquid,, reclaims ethanol, and it is standby that filtrate microwave vacuum is concentrated into the clear paste of Baume degrees 1.25～1.28 of 60 ℃ of relative densities;
(3) medicinal residues in step (1) and (2) are added again to 10 ~ 15 times of amount microwave extraction secondaries of 65% ethanol water, be heated to maintain 30 minutes after boiling at every turn, standing, filtration, merge extractive liquid,, reclaim ethanol, it is standby that filtrate is concentrated into the clear paste of Baume degrees 1.25～1.28 of 60 ℃ of relative densities;
(4) three kinds of clear paste of combining step (1), (2) and (3), being concentrated into relative density is the thick paste of the Baume degrees 1.35～1.38 of 60 ℃, microwave vacuum drying, freezing and pulverizing becomes fine powder, is prepared into the pharmaceutical composition for the treatment of anguilla japonica genus Pseudodactylogrus from Anguilla anguilla parasitic disease.
5. a preparation method for anguilla japonica genus Pseudodactylogrus from Anguilla anguilla parasitic disease medicine according to claim 1, is characterized in that: it comprises the following steps:
(1) according to amount ratio, by Ramulus Cinnamomi, Fructus Quisqualis, adopt freezing and micronizing machine to pulverize, be crushed to superfine powder more than 300 orders, standby;
(2) according to amount ratio, by the Radix Pulsatillae, Radix Et Rhizoma Rhei, Radix Sophorae Flavescentis, Herba Asari, Radix Aconiti Lateralis Preparata, Rhizoma Zingiberis, Fructus Zanthoxyli, Semen Arecae, melia, Pseudobulbus cremastrae seu pleiones, Fructus Cnidii, with 10 ~ 15 times of amount reflux, extract, secondaries of percentage by weight 65% ethanol, each microwave heating maintains 30 minutes to boiling, standing, filtration, merge extractive liquid,, reclaims ethanol, and it is standby that filtrate is concentrated into the clear paste of Baume degrees 1.25～1.28 of 60 ℃ of relative densities;
(3) medicinal residues of step (2) are added to 10 ~ 15 times, water amount microwave extraction secondaries again, be heated to maintain 30 minutes after boiling at every turn, standing, filter, collecting decoction, it is standby that microwave vacuum is concentrated into the clear paste of Baume degrees 1.25～1.28 of 60 ℃ of relative densities;
(4) two kinds of clear paste of the superfine powder of combining step (1), step (2) and (3), through microwave vacuum drying, freezing and pulverizing becomes fine powder, is prepared into the pharmaceutical composition for the treatment of anguilla japonica genus Pseudodactylogrus from Anguilla anguilla parasitic disease.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106563059A (en) * | 2016-09-29 | 2017-04-19 | 厦门华鼎动物保健品有限公司 | Insecticide for aquaculture animals |
| CN108976386A (en) * | 2018-06-04 | 2018-12-11 | 张家港市顾乐仕生活家居科技有限公司 | One kind containing natural plants antibacterial and mouldproof compound polyurethane material and preparation method thereof |
| CN110623886A (en) * | 2019-10-31 | 2019-12-31 | 山东花物堂生物科技有限公司 | Preparation method of cortex meliae extract |
| CN112294889A (en) * | 2020-11-03 | 2021-02-02 | 集美大学 | Medicine for preventing and treating eel Edwardsiellosis and preparation method thereof |
-
2013
- 2013-11-27 CN CN201310609762.7A patent/CN103623370B/en active Active
Non-Patent Citations (2)
| Title |
|---|
| 志凡等: "常用畜禽中草药驱虫保健剂的选择和配伍", 《今日科技》, 30 June 1997 (1997-06-30) * |
| 王桂宏等: "纯中药制剂鱼用快杀灵的研制", 《畜牧与兽医》, vol. 32, no. 6, 30 June 2000 (2000-06-30) * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106563059A (en) * | 2016-09-29 | 2017-04-19 | 厦门华鼎动物保健品有限公司 | Insecticide for aquaculture animals |
| CN108976386A (en) * | 2018-06-04 | 2018-12-11 | 张家港市顾乐仕生活家居科技有限公司 | One kind containing natural plants antibacterial and mouldproof compound polyurethane material and preparation method thereof |
| CN110623886A (en) * | 2019-10-31 | 2019-12-31 | 山东花物堂生物科技有限公司 | Preparation method of cortex meliae extract |
| CN112294889A (en) * | 2020-11-03 | 2021-02-02 | 集美大学 | Medicine for preventing and treating eel Edwardsiellosis and preparation method thereof |
| CN112294889B (en) * | 2020-11-03 | 2021-12-14 | 集美大学 | Medicine for preventing and treating eel Edwardsiellosis and preparation method thereof |
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