CN103608674A - 一种配位金属的气味化合物的探测方法 - Google Patents

一种配位金属的气味化合物的探测方法 Download PDF

Info

Publication number
CN103608674A
CN103608674A CN201280018147.2A CN201280018147A CN103608674A CN 103608674 A CN103608674 A CN 103608674A CN 201280018147 A CN201280018147 A CN 201280018147A CN 103608674 A CN103608674 A CN 103608674A
Authority
CN
China
Prior art keywords
olfactory receptor
copper ions
metal
receptor
olfactory
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201280018147.2A
Other languages
English (en)
Other versions
CN103608674B (zh
Inventor
庄寒异
松波宏明
艾力克·布劳克
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shanghai Jiaotong University School of Medicine
Research Foundation of State University of New York
Duke University
Original Assignee
Shanghai Jiaotong University School of Medicine
Research Foundation of State University of New York
Duke University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shanghai Jiaotong University School of Medicine, Research Foundation of State University of New York, Duke University filed Critical Shanghai Jiaotong University School of Medicine
Publication of CN103608674A publication Critical patent/CN103608674A/zh
Application granted granted Critical
Publication of CN103608674B publication Critical patent/CN103608674B/zh
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

本发明公开一种配位金属的气味化合物的探测方法，利用以HEK293T以及其衍生细胞系为基础的哺乳动物嗅觉受体的异源表达系统，高效表达诸如小鼠嗅觉受体MOR244-3等一序列嗅觉受体，在加入气味化合物的同时在体系中另外加入铜离子，利用双荧光酶或Glosensor等测量受体活化后所产生的cAMP的方法检测嗅觉受体的激活，铜离子可以特异性地增强嗅觉受体的反应，达到高效的受体活化，且这种增强作用未见于其它金属离子。本发明中利用结合铜离子的细胞及其它平台可达到高灵敏度的探测及鉴别硫类及其它可螯合金属的化合物的目的，可被应用到工业及农业的一些领域，比如天然气泄漏检测、动物跟踪等。

Description

PCT国内申请，说明书已公开。

Claims (1)

  1. PCT国内申请，权利要求书已公开。
CN201280018147.2A 2011-04-14 2012-04-13 一种配位金属的气味化合物的探测方法 Expired - Fee Related CN103608674B (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201161475386P 2011-04-14 2011-04-14
US61/475386 2011-04-14
US61/475,386 2011-04-14
PCT/CN2012/074025 WO2012139526A1 (zh) 2011-04-14 2012-04-13 一种配位金属的气味化合物的探测方法

Publications (2)

Publication Number Publication Date
CN103608674A true CN103608674A (zh) 2014-02-26
CN103608674B CN103608674B (zh) 2015-04-22

Family

ID=47008829

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201280018147.2A Expired - Fee Related CN103608674B (zh) 2011-04-14 2012-04-13 一种配位金属的气味化合物的探测方法

Country Status (2)

Country Link
CN (1) CN103608674B (zh)
WO (1) WO2012139526A1 (zh)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116200456A (zh) * 2023-04-27 2023-06-02 汉王科技股份有限公司 利用嗅觉受体检测食品变质的方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1882689A (zh) * 2003-11-21 2006-12-20 艾尼纳制药公司 产生嗅觉gpcr的方法
CN101087879A (zh) * 2004-06-18 2007-12-12 杜克大学 气味受体的调控剂
WO2009136742A1 (en) * 2008-05-07 2009-11-12 Seoul National University Industry Foundation Olfactory receptor-functionalized transistors for highly selective bioelectronic nose and biosensor using the same

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1882689A (zh) * 2003-11-21 2006-12-20 艾尼纳制药公司 产生嗅觉gpcr的方法
CN101087879A (zh) * 2004-06-18 2007-12-12 杜克大学 气味受体的调控剂
WO2009136742A1 (en) * 2008-05-07 2009-11-12 Seoul National University Industry Foundation Olfactory receptor-functionalized transistors for highly selective bioelectronic nose and biosensor using the same

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
JIANGYUN WANG ET AL: "Is the olfactory receptor a metalloprotein?", 《PNAS》, vol. 100, no. 6, 18 March 2003 (2003-03-18) *
NILMINI VISWAPRAKASH ET AL: "Enhancement of Odorant-Induced Responses in Olfactory Receptor Neuron by Zinc Nanoparticles", 《CHEMICAL SENSES》, vol. 34, no. 7, 30 September 2009 (2009-09-30) *
XUFANG DUAN ET AL: "Crucial role of copper in detection of metal-coordinating odorants", 《PNAS》, vol. 109, no. 9, 28 February 2012 (2012-02-28) *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116200456A (zh) * 2023-04-27 2023-06-02 汉王科技股份有限公司 利用嗅觉受体检测食品变质的方法
CN116200456B (zh) * 2023-04-27 2023-07-28 汉王科技股份有限公司 利用嗅觉受体检测食品变质的方法

Also Published As

Publication number Publication date
CN103608674B (zh) 2015-04-22
WO2012139526A1 (zh) 2012-10-18

Similar Documents

Publication Publication Date Title
WO2014097286A8 (en) Systems and methods for determining a chemical state
NZ615154A (en) Micro-organism threat detection
WO2012075506A3 (en) Methods for predicting and treating infection-induced illnesses and predicting the severity of infection-induced illnesses
WO2012154794A3 (en) Enzyme concentration and assays
NZ629703A (en) Methods and compositions for detecting multiple analytes with a single signal
MY160455A (en) Detection and enumeration of microorganisms
MX354465B (es) Deteccion de variacion del nucleotido en la secuencia de acido nucleico de objetivo por ensayo de desdoblamiento y extension de pto.
BR112015000911A2 (pt) iniciadores cooperativos, sondas e suas respectivas aplicações
WO2013106844A3 (en) Methods and compositions for the treatment and diaginosis of pancreatic cancer
MX2014010068A (es) Metodos y sistemas para amplificacion de señal de bioensayos.
WO2009057829A3 (en) Nucleic acid primer set for detection of drug-resistant strain of hepatitis b virus, assay kit, and method of detecting drug-resistant strain of hepatitis b virus
WO2011127001A3 (en) Quantum dot-based optical sensors for rapid detection and quantitative analysis of biomolecules and biological materials
NZ615098A (en) Genetic markers for myb28
GB201021499D0 (en) Detection of quantative genetic differnces
EA201291224A1 (ru) Способ извлечения бетаина из мелассы
WO2012034130A3 (en) Methods and compositions for nucleic acid detection
CN103608674A (zh) 一种配位金属的气味化合物的探测方法
MY176888A (en) Method for detection of target analyte(s)
WO2013029631A3 (en) Enzyme detection by microfluidics
MX337137B (es) Anticuerpos monoclonales y metodos de deteccion de enzimas que confieren resistencia a acido 2, 4-diclorofenoxiacetico.
WO2012011798A3 (en) Detection of formaldehyde
MX2018012485A (es) Sistemas y metodos para ensayos para trigliceridos electroquimicos.
Vogler et al. The impact of submarine ground water discharge on a coastal ecosystem of the southern Baltic Sea: Results from the BONUS+ project AMBER
Bae et al. Development of causal map for greenhouse gas emission in construction
Horvat et al. HCN Producing Bacteria Enable Sensing Of Non-Bioavailable Hg Species by the Whole Cell Biosensor

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20150422

Termination date: 20200413

CF01 Termination of patent right due to non-payment of annual fee