CN103599438A - Traditional Chinese medicine tincture for treating bone diseases and preparation method thereof - Google Patents
Traditional Chinese medicine tincture for treating bone diseases and preparation method thereof Download PDFInfo
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Abstract
The invention provides a traditional Chinese medicine tincture for treating bone diseases and a preparation method thereof, and relates to a traditional Chinese medicine tincture for treating diseases and a preparation method thereof. The traditional Chinese medicine tincture is used for solving the problems that the treatment and recovery period of fracture patients is long, the patients need to take analgesia anti-inflammatory medicines, gonarthrosis frequently occurs and needs to be controlled and treated as soon as possible, bone fracture medicines slowly take effect, have high toxic and side effects and are high in cost, and medicines for effectively controlling and treating the bone ununion and the bone disappear are not provided at present. The traditional Chinese medicine tincture is prepared from traditional Chinese medicine decoction pieces and an ethanol solution with the volume concentration of 50%, wherein the traditional Chinese medicine decoction pieces comprise drynaria rhizome, caulis spatholobi, smilax glabra, loranthus parasiticus, cortex acanthopanacis, camphor root, radix cudramiae, obscured homalomenarhizome and radix fici simplicissimae. The preparation method comprises the steps of steeping the traditional Chinese medicine decoction pieces in ethanol and sucking liquid supernatant. The traditional Chinese medicine tincture is used for treating the bone diseases.
Description
Technical field
The present invention relates to a kind of middle tincture for the treatment of disease and preparation method thereof.
Background technology
In state-owned sentence common saying " 100 days injured in the sinews or bones ", it is theoretical that modern hospital is also all being followed the bone injury of " Campbell ", " one dispels the swollen phase to surrounding antiinflammatory; Within five to eight weeks, connect the muscle symplectic bone phase; Nine to 12 weeks hard muscle bone strengthening phases "; If patient is worn with age or anemia of pregnant woman needs the time of more growing to recover.And pain is strong in this process, affect patient's sleep and have a rest, cause immunity and system to decline, also easily cause various inflammation.So, at present fracture patient except to take bone-setting medicament and the medicine of replenishing the calcium, also to take or inject analgesia and anti-inflammatory drug.
For comminuted fracture, be mainly to treat by the means of operation, later stage knitting still will be seen patient's self health.In actual therapeutic process, there will be the situation of bone does not connect to occur, What is more there is the situation that atrophic debility of bones loses.At present this is not had to medicine or the treatment means effectively controlling and treat.
Due to its physiological feature, easily there is pathological changes in knee joint, causes patient's inconvenient activity.For gonarthrosis, serious patient can adopt the mode of replacement joint to treat, for example metal joint or Corallium Japonicum Kishinouye joint, the Corallium Japonicum Kishinouye joint low discomfort of intensity is fit to do knee joint, although metal joint intensity is high, but be easily corroded and wear and tear human normal tissue, and also having rejection, service life is also limited.
Because of the general mode that all adopts sealing of pain of collision or contusion initiation, only cure the symptoms, not the disease; And, for non-medical personnel, be difficult to grasp and application.
The medicine for the treatment of osteopathia is many at present, but complicated component, toxic and side effects is large, and patient's internal organs and health easily sustain damage.Run into the patient who is in a bad way, high medical expense makes a lot of patients abandon the hope for the treatment of, rehabilitation.
Summary of the invention
The present invention is in order to solve fracture patient therapy rehabilitation cycle length, also to need use to ease pain and anti-inflammatory drug, gonarthrosis is multiple need control treatment early, bone injury medicine takes effect slowly, toxic and side effects is large, expense is high, and the problem of bone does not connect and the atrophic debility of bones mistake effective control of nothing at present and medicine, and a kind of middle tincture for the treatment of osteopathia providing and preparation method thereof.
The present invention treats the alcoholic solution that the middle tincture of osteopathia is 50% by the prepared slices of Chinese crude drugs and volumetric concentration and makes; The prepared slices of Chinese crude drugs are comprised of the Rhizoma Drynariae of 18～22 parts, the Caulis Spatholobi of 34～38 parts, the Rhizoma Smilacis Glabrae of 38～42 parts, the Herba Taxilli of 30～34 parts, the Cortex Acanthopancis of 22～26 parts, the Radix Cinnamomi Camphorae of 18～22 parts, the Radix Cudraniae of 22～26 parts, the Rhizoma Homalomenae of 18～22 parts and the Herba Fici Simplicissimae of 14～18 parts by weight.
The middle tincture of above-mentioned treatment osteopathia is prepared according to the following steps:
One, by prepared slices of Chinese crude drugs screenings, the weight ratio of pressing 14～18 parts of 18～22 parts of Rhizoma Drynariae, 34～38 parts of Caulis Spatholobis, 38～42 parts of Rhizoma Smilacis Glabraes, 30～34 parts of Herba Taxillis, 22～26 parts of Cortex Acanthopanciss, 18～22 parts of Radix Cinnamomi Camphoraes, 22～26 parts of Radix Cudraniaes, 18～22 parts of Rhizoma Homalomenaes and Herba Fici Simplicissimae after cleaning, pulverizing mixes, be placed in pot for solvent extraction, and then add the alcoholic solution of 10 times of prepared slices of Chinese crude drugs powder gross weights, sealing dipping after stirring and evenly mixing, total immersion stain 37 days, stirs 1～2 every day;
Two, standing absorption step 1 dipping supernatant, the middle tincture of the osteopathia that obtains medical treatment.
The middle tincture that the present invention treats osteopathia both can take orally, also can external.For oral administration: 10 minutes ante cibum are oral, each about 15ml, three times on the one; External: dip in tincture in the present invention with absorbent cotton and be applied in painful area, to this position dermal sensation heating, every day 3～4 times.
Rhizoma Drynariae---nature and flavor:: hardship, temperature.Function cures mainly: the kidney invigorating bone strengthening, continues and hinder pain relieving; For lumbago due to renal deficiency, Hiccough and deaf, odontoseisis, falling winks frustrates, injured bone; External treatment alopecia areata, vitiligo.Attention: resistance is empty and without the careful clothes of blood stasis person.
Caulis Spatholobi---nature and flavor: bitter, sweet, temperature.Function cures mainly: enrich blood, invigorate blood circulation, dredging collateral.For menoxenia, blood deficiency and yellow complexion, paralysis and numbness, rheumatic arthralgia.Attention: deficiency of YIN fire high person be cautious use of.
Rhizoma Smilacis Glabrae---nature and flavor: sweet, light, flat.Function cures mainly: dehumidifying, removing toxic substances, easing joint movement.For damp and hot stranguria with turbid discharge, leukorrhagia, carbuncle, scrofula, scabies, the limbs contracture due to syphilis and mercurialism, bones and muscles pain.Attention: the careful clothes of hepatic and renal YIN deficiency person.
Herba Taxilli---nature and flavor: bitter, sweet, flat.Function cures mainly: invigorating the liver and kidney, bone and muscle strengthening, wind-damp dispelling, antiabortive unit.For rheumatic arthralgia, soreness of the waist and knees, muscles and bones is unable, and metrorrhagia warp is many, blood leaking in gestation, frequent fetal movement; Hypertension.
Cortex Acanthopancis---nature and flavor: pungent, bitter, temperature.Function cures mainly: wind-damp dispelling, invigorating the liver and kidney, bone and muscle strengthening.For rheumatic arthralgia, muscles and bones flaccidity is soft, children's's walking retardation in children, and body void is weak, edema, beriberi.Attention: the careful clothes of hyperactivity of fire caused by deficiency of YIN person.
Radix Cinnamomi Camphorae---nature and flavor: pungent; Temperature.Function cures mainly: warming middle-JIAO to relieve pain; Ward off dirty and in; Expelling wind and removing dampness.Main gastralgia; Cholera is vomited and diarrhoea; Rheumatic arthralgia; Skin pruritus.Attention: the deficiency of vital energy has interior-heat, and person prohibits clothes.
Radix Cudraniae---nature and flavor: micro-hardship, flat.Function cures mainly: relieving cough and resolving phlegm, expelling wind and removing dampness, eliminating stasis to stop pain.For pulmonary tuberculosis, icterohepatitis, hepatosplenomegaly, Stomach duodenum ulcer, rheumatic lumbago and scelalgia; Fracture is controlled in external, traumatic injury.Attention: the medicine will > > of < < Nanning City contraindication in pregnancy.
Rhizoma Homalomenae---nature and flavor: hardship, pungent, temperature.Function cures mainly: wind-damp dispelling, muscle reinforcing and bone strengthening, promoting blood circulation and stopping pain.For anemofrigid-damp arthralgia, chills and pain of the waist and kness, lower limb contracture is numb.Attention: yin asthenia generating intrinsic heat person avoids use.
Herba Fici Simplicissimae---nature and flavor: sweet, tepor.Function cures mainly: invigorating the spleen for eliminating dampness, promoting the circulation of QI and reducing phlegm, relaxing muscles and tendons and activating QI and blood in the collateral.For pulmonary tuberculosis cough, chronic bronchitis, rheumatic arthritis, lumbago and skelalgia, insufficiency of the spleen edema, night sweat after being ill, leucorrhea.
Radix Flemingiae Philippinensis---nature and flavor: sweet, tepor, flat.Function cures mainly: wind-damp dispelling, strong waist knee joint.For rheumatic arthritis, lumbago and skelalgia, lumbar muscle strain, leucorrhea, traumatic injury.
The middle tincture that the present invention treats osteopathia has dredge the meridian passage, strong muscle symplectic bone, and stasis-dispelling and pain-killing, promotes union of fracture, treatment fracture pain, anti-inflammatory and antalgic, the effect of hemostasis, hemopoietic.Can be used for treating Bone nonunion, miscellaneous fracture, gonarthrosis, cervical spondylosis, lumbar spondylosis, hyperosteogeny, femur head necrosis, athletic injury, lumbar muscle strain, the pain that the diseases such as osteoporosis and osteomyelitis cause and arthralgia.
The middle tincture that the present invention treats osteopathia takes effect to the general external of the treatment of diseases of knee joint for 30 minutes, and oral and external can be cured for 12 weeks.
It is monarch drug that the middle tincture that the present invention treats osteopathia be take Rhizoma Drynariae and Herba Taxilli, and both play invigorating the liver and kidney altogether, bone and muscle strengthening, the continuous wound of invigorating blood circulation.Blood stored in the liver, kidney governing bones, Liver and kidney must be good for, and QI and blood biochemistry is active, the healing of skeleton self energy.Caulis Spatholobi and Radix Cudraniae, as the ministerial drug continuous muscle of can principal drug assistance invigorating blood circulation, can remove again congestion and tissue regeneration promoting blood, make blood stasis dispelling blood and just do not hinder.Rhizoma Smilacis Glabrae, Rhizoma Homalomenae, Herba Fici Simplicissimae, Radix Cinnamomi Camphorae and Cortex Acanthopancis are adjuvant drug, help all medicines of the monarch and his subjects.The middle tincture lid Liver and kidney that the present invention treats osteopathia fills, and QI and blood is smooth, and congestion goes, and fresh blood is raw, and meridians are unobstructed, and pain and swelling is from disappearing, and skeleton obtains QI and blood nutrition, and healing is from speed.Simultaneously because the medicines such as Rhizoma Smilacis Glabrae, Herba Taxilli, Cortex Acanthopancis, Radix Cinnamomi Camphorae have wind-damp dispelling concurrently, the merit of stopping numbness pain, so the middle tincture that the present invention treats osteopathia is to insensitive impediment, disease belongs to wind-cold damp pathogen seasonal menstruation network of a specified duration joint, with the passing of time becomes the disease of silts, energy giving consideration to both the incidental and fundamental.
The present invention treats the middle tincture of osteopathia can human activin stem cell, thereby promotes tissue regeneration, skeleton, cartilage, soft tissue etc. is had to the function of comprehensive reparation, can strengthen bone density.To improving body immunity, also there is certain effect.And in the present invention tincture nontoxic, harmless, have no side effect, safe and reliable.
Accompanying drawing explanation
Fig. 1 be in embodiment 2 23 years old bone does not connect male patient fracture after the X-ray in affected part.
Fig. 2 is that in embodiment 2,23 years old bone does not connect male patient takes the X-ray in tincture affected part after 27 days in the present invention.
Fig. 3 is the X-ray in 26 years old bone does not connect male patient fracture surgery affected part in embodiment 2.
Fig. 4 is the X-ray in 38 months affected parts of 26 years old bone does not connect male patient fracture surgery in embodiment 2.
Fig. 5 is the X-ray in 38 months affected parts of 26 years old bone does not connect male patient fracture surgery in embodiment 2.
Fig. 6 is that in embodiment 2,26 years old bone does not connect male patient uses the X-ray in tincture affected part after 4 weeks in the present invention.
Fig. 7 is the X-ray that in embodiment 2,26 years old bone does not connect male patient uses tincture affected part normotopia after 13 weeks in the present invention.
Fig. 8 is that in embodiment 2,26 years old bone does not connect male patient uses the X-ray of tincture side position, affected part after 13 weeks in the present invention.
The specific embodiment
Technical solution of the present invention is not limited to the following cited specific embodiment, also comprises the combination in any between each specific embodiment.
The specific embodiment one: the alcoholic solution that the middle tincture of present embodiment treatment osteopathia is 50% by the prepared slices of Chinese crude drugs and volumetric concentration is made; The prepared slices of Chinese crude drugs are comprised of the Rhizoma Drynariae of 18～22 parts, the Caulis Spatholobi of 34～38 parts, the Rhizoma Smilacis Glabrae of 38～42 parts, the Herba Taxilli of 30～34 parts, the Cortex Acanthopancis of 22～26 parts, the Radix Cinnamomi Camphorae of 18～22 parts, the Radix Cudraniae of 22～26 parts, the Rhizoma Homalomenae of 18～22 parts and the Herba Fici Simplicissimae of 14～18 parts by weight.
In the present invention, tincture has antiinflammatory (removing toxic substances), analgesic effect, so during medication, needn't have an injection and other drug treatment; During medication, patient's pain alleviates gradually, disappears.
The specific embodiment two: the difference of present embodiment and the specific embodiment one is: the prepared slices of Chinese crude drugs are comprised of the Rhizoma Drynariae of 20 parts, the Caulis Spatholobi of 36 parts, the Rhizoma Smilacis Glabrae of 40 parts, the Herba Taxilli of 32 parts, the Cortex Acanthopancis of 24 parts, the Radix Cinnamomi Camphorae of 20 parts, the Radix Cudraniae of 24 parts, the Rhizoma Homalomenae of 20 parts and the Herba Fici Simplicissimae of 16 parts by weight.Other is identical with embodiment one.
The specific embodiment three: present embodiment and the specific embodiment one or twos' difference is: also comprise the Radix Flemingiae Philippinensis of 15 parts in the prepared slices of Chinese crude drugs.Other is identical with embodiment one or two.
The specific embodiment four: the middle tincture of present embodiment treatment osteopathia is prepared according to the following steps:
One, by prepared slices of Chinese crude drugs screenings, the weight ratio of pressing 14～18 parts of 18～22 parts of Rhizoma Drynariae, 34～38 parts of Caulis Spatholobis, 38～42 parts of Rhizoma Smilacis Glabraes, 30～34 parts of Herba Taxillis, 22～26 parts of Cortex Acanthopanciss, 18～22 parts of Radix Cinnamomi Camphoraes, 22～26 parts of Radix Cudraniaes, 18～22 parts of Rhizoma Homalomenaes and Herba Fici Simplicissimae after cleaning, pulverizing mixes, be placed in pot for solvent extraction, and then add the alcoholic solution of 10 times of prepared slices of Chinese crude drugs powder gross weights, sealing dipping after stirring and evenly mixing, total immersion stain 37 days, stirs 1～2 every day;
Two, standing absorption step 1 dipping supernatant, the middle tincture of the osteopathia that obtains medical treatment.
The middle tincture of present embodiment treatment osteopathia gets final product independent external or for oral administration, also can external and coupling for oral administration.For the uncomfortable crowd who closes endo-medicine, can adopt the mode of external to treat.
The specific embodiment five: present embodiment with the difference of the specific embodiment four is: mix prepared slices of Chinese crude drugs powder by the weight ratio of 16 parts of 20 parts of Rhizoma Drynariae, 36 parts of Caulis Spatholobis, 40 parts of Rhizoma Smilacis Glabraes, 32 parts of Herba Taxillis, 24 parts of Cortex Acanthopanciss, 20 parts of Radix Cinnamomi Camphoraes, 24 parts of Radix Cudraniaes, 20 parts of Rhizoma Homalomenaes and Herba Fici Simplicissimae in step 1.Other step and parameter are identical with embodiment four.
The specific embodiment six: present embodiment with the specific embodiment four or five difference is: step 1 is mixed and also comprised the Radix Flemingiae Philippinensis of 15 parts in prepared slices of Chinese crude drugs powder.Other step and parameter are identical with embodiment three or four.
The specific embodiment seven: the difference of one of present embodiment and specific embodiment four to six is: keeping impregnation liquid temperature in step 1 dipping process is 20～35 ℃.Other step and parameter are identical with one of embodiment four to six.
The specific embodiment eight: the difference of one of present embodiment and specific embodiment four to seven is: step 1 is selected the Herba Taxilli steaming.Other step and parameter are identical with one of embodiment four to seven.
The Herba Taxilli that present embodiment steams has the effect of strengthening the loins and invigorating the kidney.
The specific embodiment nine: the difference of one of present embodiment and specific embodiment four to eight is: step 1 is selected the Rhizoma Drynariae of the system of scalding.Other step and parameter are identical with one of embodiment four to eight.
The middle tincture for the treatment of osteopathia is prepared according to the following steps:
One, by prepared slices of Chinese crude drugs screenings, the weight ratio of pressing 16 parts of 20 parts of Rhizoma Drynariae, 36 parts of Caulis Spatholobis, 40 parts of Rhizoma Smilacis Glabraes, 32 parts of Herba Taxillis, 24 parts of Cortex Acanthopanciss, 20 parts of Radix Cinnamomi Camphoraes, 24 parts of Radix Cudraniaes, 20 parts of Rhizoma Homalomenaes and Herba Fici Simplicissimae after cleaning, pulverizing mixes, be placed in pot for solvent extraction, and then add the alcoholic solution of 10 times of prepared slices of Chinese crude drugs powder gross weights, sealing dipping after stirring and evenly mixing, total immersion stain 37 days, stirs 1 every day;
Two, standing absorption step 1 dipping supernatant, the middle tincture of the osteopathia that obtains medical treatment.
Getting three batches places 3 months by normally packing to be placed under room temperature condition, detect once every month, detection method is undertaken by the method for drug quality draft standard > > and < < Pharmacopoeia of People's Republic of China > > by < < clinical research, and stability experiment result is as shown in table 1.
Table 1
| ? | 0 month | January | February | March |
| Character | Nothing | Nothing | Nothing | Nothing |
| Rhizoma Drynariae thin layer is identified | ＋ | ＋ | ＋ | ＋ |
| Herba Taxilli thin layer is identified | ＋ | ＋ | ＋ | ＋ |
| Ethanol content (%) | 49 | 49 | 49 | 49 |
| Naringin (mg/ml) | 0.03788 | 0.03512 | 0.03688 | 0.03720 |
Note: " nothing " is unchanged; "+" is positive.
Experimental result shows that in the present embodiment, tincture character is stable.
Acute toxicity test:
Select Kunming mouse, body weight is 18～22g, each serving the middle tincture 15ml that treats osteopathia with the present invention.Heating is concentrated into 4.4 times of original liquid.
Fail to find out 100 animal dead dosage.An oral administration gavage administration, dosage is by geometric progression arrangement, and maximum dose level group is calculated dosage with receptible maximum volume.Give observation post administration 7 days, none animal dead of result, fails to detect LD
50.
Mice is normal to be raised after 2 days, by 0.8ml/20g, gave tincture in the present embodiment, and in 24 hours, successive administration is 3 times, and 5 hours interval times, total is 2.4ml/20g to wanting volume.Therefore Continuous Observation 7 days, has no untoward reaction and death condition, can think the oral avirulence of tincture in the present embodiment.If calculate with adult's oral dose every day (45ml), result is normal dose 700 times, prove very safe.
Long term toxicity test:
Select Wistar germline body weight 170～180g rat to carry out long term toxicity test, low dose group press 1.5ml/kg, middle dosage group by 22.5ml/kg, high dose group by 45ml/kg gastric infusion.Normal group gavage every day 10ml/kg distilled water.Administration time is 60 days, and then then drug withdrawal observes rat main organs as convalescent period in 2 weeks, pathological tissue, and detect physiochemical indice, blood parameters.
Table 2 Rats Organs and Tissues weight
Table 3 Rats Organs and Tissues pathological tissue
Table 4 convalescent period rat body weight
Table 5 convalescent period rat serum is conventional, platelet
| Group | Quantity | Hb(g/L) | RBC(×10 12/L) | WBC(×10 9/L) | PLT(×10 9/L) |
| |
10 | 140.4±21.04 | 8.03±0.96 | 19.7±8.59 | 589.6±139.03 |
| |
10 | 146.7±4.08 | 8.66±0.29 | 21.3±3.18 | 673.9±65.04 |
| |
10 | 144.3±5.17 | 8.69±0.36 | 17.7±4.63 | 651.4±62.37 |
| |
10 | 142.8±11.81 | 8.52±0.44 | 18.9±4.63 | 550.3±233.52 |
WBC differential counting in table 6 convalescent period rat serum
| Group | Quantity | L | S | M | E | |
| Normal control | ||||||
| 10 | 78.9±4.32 | 15.8±3.21 | 5.3±1.45 | 0.07±0.22 | 0.12±0.10 | |
| |
10 | 78.5±6.21 | 15.4±3.34 | 6.2±2.95 | 0 | 0.08±0.10 |
| |
10 | 75.5±4.68 | 17.7±3.11 | 6.8±1.93 | 0 | 0.16±0.08 |
| |
10 | 77.2±4.98 | 16.6±3.48 | 6.2±2.53 | 0 | 0.10±0.11 |
Table 7 convalescent period rat blood biochemical indicator
| Biochemical indicator | Quantity | Normal control | Low dose group | Middle dosage group | High dose group |
| ALT(U/L) | 10 | 65.4±11.35 | 54.6±8.10 | 60.7±12.02 | 74.6±23.10 |
| AST(U/L) | 10 | 208.9±29.11 | 166.3±26.17 | 170.1±29.49 | 202.6±50.37 |
| BUN(mmol/L) | 10 | 6.05±0.98 | 5.78±0.68 | 5.53±0.48 | 5.74±0.53 |
| ALP(U/L) | 10 | 181.1±39.02 | 178.2±76.61 | 169.8±36.94 | 199.7±73.81 |
| Crea(μmol/L) | 10 | 74.6±11.70 | 71.1±11.94 | 67.3±10.59 | 73.2±8.64 |
| T-CHO(mmol/L) | 10 | 1.50±0.23 | 1.68±0.30 | 1.66±0.13 | 1.59±0.17 |
| GLU(mmol/L) | 10 | 7.5±2.02 | 5.9±0.71 | 5.7±0.63 | 6.3±1.79 |
| TP(g/L) | 10 | 75.1±5.83 | 79.2±7.56 | 78.2±3.97 | 77.2±3.33 |
| ALB(g/L) | 10 | 38.4±3.00 | 40.2±4.75 | 38.7±1.92 | 38.8±2.60 |
| T-BIL(μmol/L) | 10 | 4.2±1.88 | 5.6±1.87 | 5.1±1.58 | 5.3±1.84 |
According to above experimental data, can find that the middle tincture that the present invention treats osteopathia changes no significant difference to each group rat body weight; Hematology's indices is affected without significance; In blood parameters, except AST, the GLU of ALT, AST, GLU and the middle dosage group of low dose group is than the obvious reduction of normal control, other indices are had no significant effect; Rat main organs histopathologic examination no abnormality seen.It is little that result shows that the present invention treats the middle tincture prolonged application toxicity of osteopathia, drug safety.
Rhizoma Smilacis Glabrae and Herba Taxilli have toxicity, and Rhizoma Homalomenae is slightly poisonous.But the middle tincture of preparing according to compatibility of the present invention and method is for oral administration safe and reliable according to experimental results show that, there is no toxicity and any untoward reaction.
Animal effect experiment:
By healthy rat random packet, 10 every group, administration respectively.Normal control: every day, gavage gave normal saline; Model group: give retinoic acid 70mg/kg/ day moulding, do not give medicine; Low dosage administration group: every day, gavage gave retinoic acid 70mg/kg/ day, gavage is treated the middle tincture 0.9ml/kg/ day of osteopathia; Middle dosed administration group: every day, gavage gave retinoic acid 70mg/kg/ day, gavage is treated the middle tincture 1.8ml/kg/ day of osteopathia; High dose administration group: every day, gavage gave retinoic acid 70mg/kg/ day, gavage is treated the middle tincture 4.5ml/kg/ day of osteopathia; Positive control: every day, gavage gave retinoic acid 70mg/kg/ day, gavage gives iliacus red sheet 0.1g/kg/ day.Except normal control, respectively organize retinoic acid administration and after 2 weeks, stop administration moulding, the red sheet of the middle tincture for the treatment of osteopathia and iliacus continues administration 2 weeks.
Endurance experimental result is as shown in table 8:
Table 8
| Group | Quantity | The endurance |
| Normal control | ||
| 10 | 30.2±4.3 | |
| |
10 | 9.9±2.5 |
| Low |
10 | 18.9±1.1 |
| Middle dosed |
10 | 22.8±2.6 |
| High |
10 | 26.2±5.5 |
| |
10 | 17.6±4.1 |
Impact on bone density is as shown in table 9:
Table 9
Experimental data shows to take endurance time significant prolongation after the middle tincture that the present invention treats osteopathia, and bone calcium density increases.
Rat random packet, 10 every group, administration respectively.Normal control: every day, gavage gave normal saline; Low dosage administration group: every day, gavage was treated the middle tincture 0.9ml/kg/ day of osteopathia; Middle dosed administration group: every day, gavage was treated the middle tincture 1.8ml/kg/ day of osteopathia; High dose administration group: every day, gavage was treated the middle tincture 4.5ml/kg/ day of osteopathia; Positive control: every day, gavage gave " three-flower " bone-knitting powder 0.1g/kg/ day.Each organizes rat at first day difference morning and afternoon gastric infusion once.After the next morning administration 30min, with dimethylbenzene cotton balls, contact left ear 5s, the de-neck of rat after 15min and dead.With card punch, the same position of rat ears homalographic is cut, balance is weighed.Take auris dextra weight as 100%, calculate the inflammation weightening finish percentage rate cause scorching ear, result is as shown in table 10, shows that middle tincture that the present invention treats osteopathia has the effect of obvious minimizing inflammation rate of body weight gain.
Table 10
| Group | Quantity | Inflammation rate of body weight gain (%) |
| |
10 | 54.5±9.0 |
| |
10 | 44.8±4.1 |
| High |
10 | 40.2±3.2 |
| Middle dosed |
10 | 40.8±5.1 |
| Low |
10 | 42.2±8.9 |
Rat random packet, 10 every group, administration respectively.Normal control: every day, gavage gave normal saline; Low dosage administration group: every day, gavage was treated the middle tincture 0.9ml/kg/ day of osteopathia; Middle dosed administration group: every day, gavage was treated the middle tincture 1.8ml/kg/ day of osteopathia; High dose administration group: every day, gavage was treated the middle tincture 4.5ml/kg/ day of osteopathia; Positive control: every day, gavage gave " three-flower " bone-knitting powder 0.1g/kg/ day.Administration 3 days, once a day, the acetic acid solution of 30min pneumoretroperitoneum injection 5% after administration in the 3rd day (0.2ml/ only), observes every health twisting number of times in rat 15min, observed result is as shown in table 11, shows that middle tincture that the present invention treats osteopathia has the effect of anti-inflammatory and antalgic.
Table 11
| Group | Quantity | Twisting number of times |
| |
10 | 35.2±5.9 |
| |
10 | 22.3±6.2 |
| High |
10 | 17.6±5.1 |
| Middle dosed |
10 | 19.4±3.2 |
| Low |
10 | 21.3±4.6 |
Experiment also finds that the rat mononuclear phagocyte function of taking tincture in the present invention obviously strengthens, and the weight of immune organ thymus is significantly increased, and phagocytic index and thymus index are apparently higher than blank rat.Illustrate that in the present invention, tincture has the effect that improves immunity.
Embodiment 2
The middle tincture for the treatment of osteopathia is prepared according to the following steps:
One, by prepared slices of Chinese crude drugs screenings, the weight ratio of pressing 16 parts of 20 parts of Rhizoma Drynariae, 36 parts of Caulis Spatholobis, 40 parts of Rhizoma Smilacis Glabraes, 32 parts of Herba Taxillis, 24 parts of Cortex Acanthopanciss, 20 parts of Radix Cinnamomi Camphoraes, 24 parts of Radix Cudraniaes, 20 parts of Rhizoma Homalomenaes and Herba Fici Simplicissimae after cleaning, pulverizing mixes, be placed in pot for solvent extraction, and then add the alcoholic solution of 10 times of prepared slices of Chinese crude drugs powder gross weights, sealing dipping after stirring and evenly mixing, total immersion stain 37 days, stirs 1 every day;
Two, standing absorption step 1 dipping supernatant, the middle tincture of the osteopathia that obtains medical treatment.
In the present invention, tincture causes double knee joint disease, synovitis, local joint swelling (comprising gout) handicappedly has an effect taking effect fast.
Sick (comprising rheumatism, rheumatoid etc.) patient's 400 examples of double knee joint, synovitis patient's 200 examples, local joint swelling (comprising gout) patient 100 examples, affected part (having obvious pain to feel) smear middle tincture 30 that the present invention treats osteopathia clearly demarcated after 700 routine patients all feel to smear the present invention in the affected part heating of tincture, pain disappears substantially, and joint motion obviously improves.(for oral administration: 10 minutes ante cibum are oral, each about 15ml, three times on the one by for oral administration and external; External: dip in tincture in the present invention with absorbent cotton and be applied in painful area, to this position dermal sensation heating, every day 3～4 times.) the mode medication simultaneously carried out 12 weeks, 700 routine patients' foci disappearance, joint motions are flexible, and during motion, without sense of discomfort, without pain, and bone density, intensity significantly improve.
In the present invention, tincture has good therapeutic effect to fracture, bone does not connect and soft tissue injury.
Fracture patient 350 examples, soft tissue injury patient 350 examples, according to state of an illness weight, for oral administration and external (is taken orally: 10 minutes ante cibum are oral, each about 15ml, three times on the one; External: dip in tincture in the present invention with absorbent cotton and be applied in painful area, to this position dermal sensation heating, every day 3～4 times.) fracture patient uses tincture treatment fracture in the present invention after resetting, 1～2 course for the treatment of of medication, be 28 days each course for the treatment of, and effective percentage reaches 95%(fracture effectively: Clinical X mating plate has callus to generate, and bone trabecula is by fracture line, the disappearance of swelling and ache, fracture is without tenderness); Cure rate reaches 90%(and cures: patient recovers normal activity).Tincture treatment soft tissue injury patient in the present invention, the medication disappearance of swelling and ache for 1 week, cure rate is more than 90%; 1 course for the treatment of of medication, be 28 days the course for the treatment of, and cure rate reaches 100%.
Bone does not connect patient 10 examples (comprising occurring that skeleton is etched patient's 2 examples after fracture) are for oral administration and external is (for oral administration: 10 minutes ante cibum are oral, each about 15ml, three times on the one; External: dip in tincture in the present invention with absorbent cotton and be applied in painful area, to this position dermal sensation heating, every day 3～4 times.) tincture 10～12 months in the present invention, it is effective that effective percentage reaches 100%(: have callus to generate, osteogenesis is obvious), cure rate reaches 100%(and cures: union of fracture, bone trabecula, by fracture line, has seriality callus to pass through fracture line).
, after fracture, there is bone trabecula misgrowth in bone does not connect male patient 23 years old, Decrease of Bone Mineral Density, and bone fibres can not normally generate (as shown in Figure 1).Take in the present invention tincture after 27 days bone trabecula have obvious generation, bone density increases, bone fibres normally generates (as shown in Figure 2).In proof the present invention, tincture has excellent therapeutic effect to bone does not connect and osseous tissue misgrowth.
Bone does not connect (even occurring that atrophic debility of bones loses) 26 years old male of male patient, fracture surgery is as shown in Figure 3; Broach ruptures afterwards, occurs again bone does not connect, so adopt fixedly affected part of external fixer, and the multiple medical procedure such as bone grafting have been carried out, but the problem of bone does not connect still exists (as shown in Figure 4), fracture face is without growth vestige, and the clinical symptoms of losing with atrophic debility of bones.In order to promote osteogenesis to adopt mechanical stretching to stimulate osteocyte propagation, but the symptom that bone does not connect and atrophic debility of bones lose does not take a turn for the better (as shown in Figure 5).Take in the present invention tincture after 4 weeks, proximal femur union of fracture, bone density strengthens (as shown in Figure 6), takes again afterwards in the present invention tincture 9 weeks, obviously see that growth of spur, bone fibres generate, bone trabecula is connected, and medullary cavity communicates, fracture heals (as shown in Figure 7 and Figure 8) substantially.In proof the present invention tincture to bone does not connect even atrophic debility of bones lose the effect with healing.
The bone injury that in the present invention, tincture causes lumbar muscle strain, neuritis, muscle injury and a variety of causes is all effective in cure.Particularly collide, dampen the bone injury (except fracture) causing and be coated with outward with tincture in the present invention, 30min takes effect, and pain disappears substantially, can reactivate fast.
According to bone does not connect patient's treatment situation, can determine in the present invention that tincture can human activin stem cell, promote osteanagenesis.
Claims (9)
1. treat a middle tincture for osteopathia, the alcoholic solution that the middle tincture that it is characterized in that treating osteopathia is 50% by the prepared slices of Chinese crude drugs and volumetric concentration is made; The prepared slices of Chinese crude drugs are mainly comprised of the Rhizoma Drynariae of 18～22 parts, the Caulis Spatholobi of 34～38 parts, the Rhizoma Smilacis Glabrae of 38～42 parts, the Herba Taxilli of 30～34 parts, the Cortex Acanthopancis of 22～26 parts, the Radix Cinnamomi Camphorae of 18～22 parts, the Radix Cudraniae of 22～26 parts, the Rhizoma Homalomenae of 18～22 parts and the Herba Fici Simplicissimae of 14～18 parts by weight.
2. a kind of middle tincture for the treatment of osteopathia according to claim 1, is characterized in that the prepared slices of Chinese crude drugs are comprised of the Rhizoma Drynariae of 20 parts, the Caulis Spatholobi of 36 parts, the Rhizoma Smilacis Glabrae of 40 parts, the Herba Taxilli of 32 parts, the Cortex Acanthopancis of 24 parts, the Radix Cinnamomi Camphorae of 20 parts, the Radix Cudraniae of 24 parts, the Rhizoma Homalomenae of 20 parts and the Herba Fici Simplicissimae of 16 parts by weight.
3. a kind of middle tincture for the treatment of osteopathia according to claim 1 and 2, is characterized in that also comprising in the prepared slices of Chinese crude drugs Radix Flemingiae Philippinensis of 15 parts.
4. described in preparation claim 1, treat the method for the middle tincture of osteopathia, it is characterized in that the middle tincture for the treatment of osteopathia is prepared according to the following steps:
One, by prepared slices of Chinese crude drugs screenings, the weight ratio of pressing 14～18 parts of 18～22 parts of Rhizoma Drynariae, 34～38 parts of Caulis Spatholobis, 38～42 parts of Rhizoma Smilacis Glabraes, 30～34 parts of Herba Taxillis, 22～26 parts of Cortex Acanthopanciss, 18～22 parts of Radix Cinnamomi Camphoraes, 22～26 parts of Radix Cudraniaes, 18～22 parts of Rhizoma Homalomenaes and Herba Fici Simplicissimae after cleaning, pulverizing mixes, be placed in pot for solvent extraction, and then add the alcoholic solution of 10 times of prepared slices of Chinese crude drugs powder gross weights, sealing dipping after stirring and evenly mixing, total immersion stain 37 days, stirs 1～2 every day;
Two, standing absorption step 1 dipping supernatant, the middle tincture of the osteopathia that obtains medical treatment.
5. the preparation method of the middle tincture for the treatment of osteopathia according to claim 4, is characterized in that in step 1 mixing prepared slices of Chinese crude drugs powder by the weight ratio of 16 parts of 20 parts of Rhizoma Drynariae, 36 parts of Caulis Spatholobis, 40 parts of Rhizoma Smilacis Glabraes, 32 parts of Herba Taxillis, 24 parts of Cortex Acanthopanciss, 20 parts of Radix Cinnamomi Camphoraes, 24 parts of Radix Cudraniaes, 20 parts of Rhizoma Homalomenaes and Herba Fici Simplicissimae.
6. the preparation method of the middle tincture for the treatment of osteopathia according to claim 4, is characterized in that in step 1 dipping process, keeping impregnation liquid temperature is 20～35 ℃.
7. the preparation method of the middle tincture for the treatment of osteopathia according to claim 4, is characterized in that step 1 selects the Herba Taxilli steaming.
8. the preparation method of the middle tincture for the treatment of osteopathia according to claim 4, is characterized in that step 1 selects the Rhizoma Drynariae of the system of scalding.
9. the preparation method of the middle tincture for the treatment of osteopathia according to claim 4, is characterized in that also comprising the Radix Flemingiae Philippinensis of 15 parts in step 1 mixing prepared slices of Chinese crude drugs powder.
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| CN104547794A (en) * | 2015-02-13 | 2015-04-29 | 刘正东 | Traditional Chinese medicine composition for treating bone fracture |
| CN105250896A (en) * | 2015-10-15 | 2016-01-20 | 王仕珍 | Hyperostosis medicinal liquor and preparation method thereof |
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| CN101185672A (en) * | 2007-12-19 | 2008-05-28 | 崔月清 | Orally-administered traditional Chinese medicine decoction for treating lumbar hyperosteogeny |
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| CN101185672A (en) * | 2007-12-19 | 2008-05-28 | 崔月清 | Orally-administered traditional Chinese medicine decoction for treating lumbar hyperosteogeny |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104547794A (en) * | 2015-02-13 | 2015-04-29 | 刘正东 | Traditional Chinese medicine composition for treating bone fracture |
| CN105250896A (en) * | 2015-10-15 | 2016-01-20 | 王仕珍 | Hyperostosis medicinal liquor and preparation method thereof |
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