Summary of the invention
Technical problem to be solved by this invention is to provide a kind of for improving Chinese medicine composition feed addictive and the feed of broiler chicken ketoboidies quality and local flavor, and it has low toxicity, has no side effect, the effect of noresidue, can significantly improve the ketoboidies quality of broiler chicken.
For solving the problems of the technologies described above, the invention provides a kind ofly for improving the feed of broiler chicken ketoboidies quality and local flavor, it comprises main feed composition, galactomannan, betaine, nicotinate chromium, soybean lecithin, Bee Pollen, salt, bone meal, sodium bicarbonate, alfalfa meal, amino acid additive, vitamin(e) supplement, Chinese herbal feed additive and microorganism formulation.
Wherein, in described feed, the weight portion of each material composition is respectively main feed composition 90 weight portion~110 weight portions, galactomannan 2 weight portion~4 weight portions, betaine 6 weight portion~8 weight portions, nicotinate chromium 1 weight portion~3 weight portion, soybean lecithin 2 weight portion~4 weight portions, Bee Pollen 3 weight portion~5 weight portions, salt 1 weight portion~3 weight portion, bone meal 3 weight portion~5 weight portions, sodium bicarbonate 1 weight portion~3 weight portion, alfalfa meal 5 weight portion~7 weight portions, amino acid additive 4 weight portion~6 weight portions, vitamin(e) supplement 4 weight portion~6 weight portions, Chinese herbal feed additive 15 weight portion~17 weight portions and microorganism formulation 2 weight portion~4 weight portions.
Wherein, the bulk drug that described Chinese herbal feed additive comprises has the Radix Astragali, the bighead atractylodes rhizome, kuh-seng, the membrane of a chicken's gizzard, hawthorn, the fruit of Cherokee rose, Chinese gall, aleppo avens, wood indigo plant, pilose gerbera herb, Virgin's milk mushroom, galangal, garlic, nutmeg, sea-buckthorn, rhizoma atractylodis, Radix Glycyrrhizae, sealwort, the stem of noble dendrobium, the fruit of glossy privet, giant knotweed, Ligusticum wallichii, west safflower, suchow mosla herb, storax, golden cypress and the banksia rose.
Further, described Chinese herbal feed additive only consists of above-mentioned raw materials medicine.
In described Chinese herbal feed additive, the weight portion of each bulk drug is respectively the Radix Astragali 55 weight portion~65 weight portions, the bighead atractylodes rhizome 35 weight portion~45 weight portions, kuh-seng 20 weight portion~30 weight portions, the membrane of a chicken's gizzard 20 weight portion~30 weight portions, hawthorn 35 weight portion~45 weight portions, the fruit of Cherokee rose 25 weight portion~35 weight portions, Chinese gall 30 weight portion~40 weight portions, aleppo avens 45 weight portion~55 weight portions, blue 20 weight portion~30 weight portions of wood, pilose gerbera herb 25 weight portion~35 weight portions, Virgin's milk mushroom 30 weight portion~40 weight portions, galangal 35 weight portion~45 weight portions, garlic 25 weight portion~35 weight portions, nutmeg 30 weight portion~40 weight portions, sea-buckthorn 25 weight portion~35 weight portions, rhizoma atractylodis 20 weight portion~30 weight portions, Radix Glycyrrhizae 25 weight portion~35 weight portions, sealwort 30 weight portion~40 weight portions, the stem of noble dendrobium 25 weight portion~35 weight portions, the fruit of glossy privet 30 weight portion~40 weight portions, giant knotweed 35 weight portion~45 weight portions, Ligusticum wallichii 20 weight portion~30 weight portions, west safflower 25 weight portion~35 weight portions, suchow mosla herb 30 weight portion~40 weight portions, storax 25 weight portion~35 weight portions, golden cypress 45 weight portion~55 weight portions and the banksia rose 25 weight portion~35 weight portions.
Further preferably, in described Chinese herbal feed additive, the weight portion of each bulk drug is respectively the Radix Astragali 58 weight portion~62 weight portions, the bighead atractylodes rhizome 38 weight portion~42 weight portions, kuh-seng 23 weight portion~27 weight portions, the membrane of a chicken's gizzard 23 weight portion~27 weight portions, hawthorn 38 weight portion~42 weight portions, the fruit of Cherokee rose 28 weight portion~32 weight portions, Chinese gall 33 weight portion~37 weight portions, aleppo avens 48 weight portion~52 weight portions, blue 23 weight portion~27 weight portions of wood, pilose gerbera herb 28 weight portion~32 weight portions, Virgin's milk mushroom 33 weight portion~37 weight portions, galangal 38 weight portion~42 weight portions, garlic 28 weight portion~32 weight portions, nutmeg 33 weight portion~37 weight portions, sea-buckthorn 28 weight portion~32 weight portions, rhizoma atractylodis 23 weight portion~27 weight portions, Radix Glycyrrhizae 28 weight portion~32 weight portions, sealwort 33 weight portion~37 weight portions, the stem of noble dendrobium 28 weight portion~32 weight portions, the fruit of glossy privet 33 weight portion~37 weight portions, giant knotweed 38 weight portion~42 weight portions, Ligusticum wallichii 23 weight portion~27 weight portions, west safflower 28 weight portion~32 weight portions, suchow mosla herb 33 weight portion~37 weight portions, storax 28 weight portion~32 weight portions, golden cypress 48 weight portion~52 weight portions and the banksia rose 28 weight portion~32 weight portions.
Most preferably, in described Chinese herbal feed additive, the weight portion of each bulk drug is respectively the Radix Astragali 60 weight portions, the bighead atractylodes rhizome 40 weight portions, kuh-seng 25 weight portions, the membrane of a chicken's gizzard 25 weight portions, hawthorn 40 weight portions, the fruit of Cherokee rose 30 weight portions, Chinese gall 35 weight portions, aleppo avens 50 weight portions, blue 25 weight portions of wood, pilose gerbera herb 30 weight portions, Virgin's milk mushroom 35 weight portions, galangal 40 weight portions, garlic 30 weight portions, nutmeg 35 weight portions, sea-buckthorn 30 weight portions, rhizoma atractylodis 25 weight portions, Radix Glycyrrhizae 30 weight portions, sealwort 35 weight portions, the stem of noble dendrobium 30 weight portions, the fruit of glossy privet 35 weight portions, giant knotweed 40 weight portions, Ligusticum wallichii 25 weight portions, west safflower 30 weight portions, suchow mosla herb 35 weight portions, storax 30 weight portions, golden cypress 50 weight portions and the banksia rose 30 weight portions.
Wherein, the preparation method of described Chinese herbal feed additive, is specially:
Above-mentioned raw materials medicine component is mixed by weight, obtain mixture, put into reactor, add the water with respect to 3~5 times of mixture qualities, slow fire heating decocts 2~4 hours, and decoction liquor is filtered, filter residue adds the water with respect to 2~4 times of the mixture qualities obtaining above, and slow fire boiling is 1~3 hour again, and decoction liquor is filtered again, filtered fluid is merged, concentrated except anhydrating, obtain lotion, dry, pulverize, obtain Chinese herbal feed additive.
The present invention also provides the preparation method of above-mentioned feed, and it comprises:
The first step, get 3kg microbe additive and join in 50kg~100kg water and mix, obtain zymotic fluid, the zymotic fluid that 100kg is obtained joins in 100kg main feed composition, evenly pack sealing, is placed in the environment of 20 ℃~30 ℃ and obtains fermentation material after 10~20 days;
Second step, prepares Chinese herbal feed additive according to the method described above;
The 3rd step, together with the Chinese herbal feed additive that the fermentation material that the first step is obtained obtains with second step and other components, put into fodder mixing machine, continuous uniform stirred after 1~3 hour, putting into pulverizer tentatively pulverizes, after taking-up, putting into micronizer, to carry out separating twice be fine grained again, the feed that gets product after sterilization, sealing, pack of the fine grained after pulverizing.
It is a kind of for improving the Chinese herbal feed additive of meat quality of table poultry that the present invention also provides, and it bulk drug comprising has the Radix Astragali, the bighead atractylodes rhizome, kuh-seng, the membrane of a chicken's gizzard, hawthorn, the fruit of Cherokee rose, Chinese gall, aleppo avens, wood indigo plant, pilose gerbera herb, Virgin's milk mushroom, galangal, garlic, nutmeg, sea-buckthorn, rhizoma atractylodis, Radix Glycyrrhizae, sealwort, the stem of noble dendrobium, the fruit of glossy privet, giant knotweed, Ligusticum wallichii, west safflower, suchow mosla herb, storax, golden cypress and the banksia rose.
Wherein, in described Chinese herbal feed additive, the weight portion of each bulk drug is respectively the Radix Astragali 55 weight portion~65 weight portions, the bighead atractylodes rhizome 35 weight portion~45 weight portions, kuh-seng 20 weight portion~30 weight portions, the membrane of a chicken's gizzard 20 weight portion~30 weight portions, hawthorn 35 weight portion~45 weight portions, the fruit of Cherokee rose 25 weight portion~35 weight portions, Chinese gall 30 weight portion~40 weight portions, aleppo avens 45 weight portion~55 weight portions, blue 20 weight portion~30 weight portions of wood, pilose gerbera herb 25 weight portion~35 weight portions, Virgin's milk mushroom 30 weight portion~40 weight portions, galangal 35 weight portion~45 weight portions, garlic 25 weight portion~35 weight portions, nutmeg 30 weight portion~40 weight portions, sea-buckthorn 25 weight portion~35 weight portions, rhizoma atractylodis 20 weight portion~30 weight portions, Radix Glycyrrhizae 25 weight portion~35 weight portions, sealwort 30 weight portion~40 weight portions, the stem of noble dendrobium 25 weight portion~35 weight portions, the fruit of glossy privet 30 weight portion~40 weight portions, giant knotweed 35 weight portion~45 weight portions, Ligusticum wallichii 20 weight portion~30 weight portions, west safflower 25 weight portion~35 weight portions, suchow mosla herb 30 weight portion~40 weight portions, storax 25 weight portion~35 weight portions, golden cypress 45 weight portion~55 weight portions and the banksia rose 25 weight portion~35 weight portions.
The preparation method of described Chinese herbal feed additive, is specially:
Above-mentioned raw materials medicine component is mixed by weight, obtain mixture, put into reactor, add the water with respect to 3~5 times of mixture qualities, slow fire heating decocts 2~4 hours, and decoction liquor is filtered, filter residue adds the water with respect to 2~4 times of the mixture qualities obtaining above, and slow fire boiling is 1~3 hour again, and decoction liquor is filtered again, filtered fluid is merged, concentrated except anhydrating, obtain lotion, dry, pulverize, obtain Chinese herbal feed additive.
Beneficial effect of the present invention:
Provided by the invention for improving Chinese medicine composition feed addictive and the feed of broiler chicken ketoboidies quality and local flavor, it has low toxicity, has no side effect, the effect of noresidue, can significantly improve ketoboidies quality and the local flavor of broiler chicken.
The specific embodiment
The trophism of Chinese herbal feed additive is mainly reflected in and in Chinese herbal medicine, contains the necessary various nutriments of animal growth, as protein, amino acid, vitamin, trace element etc., thereby can promote diet nutrient full price, in Chinese herbal medicine, contain various active composition, there is sterilization, antibacterial effect, to Escherichia coli, yellow gold-coloured staphylococci, Diplococcus pneumopniae, streptococcus all has certain inhibitory action, polysaccharide in Chinese herbal medicine, alkaloid, organic acid volatilization wet goods has the immune effect of enhancing, Chinese herbal medicine can also improve collective defense resistance and regulate the sharp former effect that relaxes, body is regulated and improves physiological function, stop stress reaction, play anti-stress effect, natural Chinese medicinal herb itself does not contain vitamin ingredients, but play vitamin function, Chinese herbal medicine contains abundant bioactivator, can regulate the anti-disease physiological mechanism in body, improve the non-specific and specific immune function of livestock and poultry, strengthen on the whole the protection capability of body or there is immunologic enhancement.
The invention provides a kind ofly for improving the feed of broiler chicken ketoboidies quality and local flavor, it comprises main feed composition, galactomannan, betaine, nicotinate chromium, soybean lecithin, Bee Pollen, salt, bone meal, sodium bicarbonate, alfalfa meal, amino acid additive, vitamin(e) supplement, Chinese herbal feed additive and microorganism formulation.
Wherein, in described feed, the weight portion of each material composition is respectively main feed composition 90 weight portion~110 weight portions, galactomannan 2 weight portion~4 weight portions, betaine 6 weight portion~8 weight portions, nicotinate chromium 1 weight portion~3 weight portion, soybean lecithin 2 weight portion~4 weight portions, Bee Pollen 3 weight portion~5 weight portions, salt 1 weight portion~3 weight portion, bone meal 3 weight portion~5 weight portions, sodium bicarbonate 1 weight portion~3 weight portion, alfalfa meal 5 weight portion~7 weight portions, amino acid additive 4 weight portion~6 weight portions, vitamin(e) supplement 4 weight portion~6 weight portions, Chinese herbal feed additive 15 weight portion~17 weight portions and microorganism formulation 2 weight portion~4 weight portions.
Further preferably, in described feed, the weight portion of each material composition is respectively main feed composition 100 weight portions, galactomannan 3 weight portions, betaine 7 weight portions, nicotinate chromium 2 weight portions, soybean lecithin 3 weight portions, Bee Pollen 4 weight portions, salt 2 weight portions, bone meal 4 weight portions, sodium bicarbonate 2 weight portions, alfalfa meal 6 weight portions, amino acid additive 5 weight portions, vitamin(e) supplement 5 weight portions, Chinese herbal feed additive 16 weight portions and microorganism formulation 3 weight portions.
Galactomannans is a kind of biological species animal immunity regulator, has the animal intestinal of adjusting microorganism, improves animal autoimmunity ability, and prevention animal intestinal disease, improves fertility, improves price of deed power, improves the effect of meat.
Betaine can promote fatty decomposition by improving the content of carnitine, thereby reduce accumulating of body fat, improve lean meat percentage, betaine can improve creatine and myoglobin content in muscle, and creatine is the important as precursors thing of chicken flavor, creatinine is most important delicate flavour material in muscle, and myoglobins and muscle color are closely related, therefore, significantly improve the chicken meat quality of broiler chicken.
Nicotinate chromium is that trivalent chromium is combined with nicotinic acid and is formed, and has the physiological action of glucose tolerance factor, can improve the activity of insulin in animal and human body, can improve ketoboidies quality.
Soybean lecithin is the mixture of various phosphoglycerides and derivative thereof in soybean, it is the bioactivator with different physiological roles, mainly comprise lecithin, cephalin, phosphatidylinositols and serinephosphatide, soybean lecithin has the integrality of Cell protection membrane structure and function, anti-oxidant, reducing blood lipid, the effects such as regulation and control fat metabolism, can improve feed intake and the production performance of poultry, improve the health status of poultry, soybean lecithin adds in mixed feed can reduce the dust in mixed feed, thereby reduce the generation of livestock and birds respiratory disease, can prevent the automatic classification of dust from flying and feed, to keeping feed mixture homogeneity to there is good effect, the property balanced in nutrition that has kept daily ration, phosphatide has good lubricity, can reduce equipment attrition, reduce energy consumption simultaneously, and the temperature arrising caused by friction that makes feed reduces to minimum, phospholipid molecule has hydrophilic and two groups of oleophylic, therefore phosphatide is good fat emulsifier, can protect the unrighted acid in feed, improve the Quality and yield of granulating, can save the consumption of poultry to methionine, a large amount of liposoluble vitamins is provided, thereby can improve antioxidant levels fatty in feed.
Bee Pollen contains abundant amino acid, organized enzyme, vitamin, trace element and auxin etc., can supplement and balanced feed in essential nutritional labeling, be conducive to digesting and assimilating of body, promote growth, improve weightening finish, minimizing feedstuff-meat ratio.And that propolis contains corresponding glue source plant is similar, the concentrate of mass efficient active component, can accelerate the metabolic process of body, promotes growing of body.
Microorganism formulation, in the process of fermented feed, generates on the one hand carbohydrate, amino acids, a large number of nutrients such as protein-based, and it can directly increase the nutrition of cultivation body body; On the other hand, enter the dominant population that cultivation body enteron aisle forms useful viable bacteria, can improve the enteric microorganism ecosystem; Each beneficial bacterium can, by a series of biochemical reactions, produce physiological activator again under good condition.As various digestive ferments, metabolic enzyme, endocrine hormone etc., not only strengthen anti pathologic immunity ability, reduce disease, but also accelerate, to the digesting and assimilating of feed nutrient, to promote growth, put on weight, the price of deed improves.In addition, colony house stink alleviates, environmental sanitary quality improves, also be conducive to growing up healthy and sound of livestock, described microorganism formulation can be Lactobacillus casei, lactobacillus lactis, Lactobacillus plantarum, lactoenterococcus, Pediococcus pentosaceus, yeast, candida utili, swamp Rhodopseudomonas, bacillus subtilis, bacillus licheniformis, lactic acid bacteria, one or more combine, as source health precious (Shandong Liuhe Farming Science and Technology Park Co., Ltd provides), this microorganism formulation primary raw material comprises product enzyme bacillus, lactic acid bacteria, saccharomycete, zytase, mannase, glucolase, amylase etc., effective viable bacteria content >=10,000,000,000/gram.
Feed ingredient comprises one or more mixing in corn, jowar, wheat bran, dregs of beans, at least has corn.
Amino acid additive comprises the various conventional amino acid such as serine, glutamic acid, glycine, isoleucine, leucine, alanine, proline, also can adopt amino acid premix, the composite aminophenol powder that as above sea cowry energy Chemical Co., Ltd. produces.
Vitamin(e) supplement comprises the various conventional vitamins such as riboflavin, vitamin C, vitamin E, vitamin A, Cobastab, also can adopt vitamin premix, as plain in the excellent dimension that Beijing You Libao Bioisystech Co., Ltd produces.
Further, described feed only consists of above-mentioned raw materials.
The bulk drug that described Chinese herbal feed additive comprises has the Radix Astragali, the bighead atractylodes rhizome, kuh-seng, the membrane of a chicken's gizzard, hawthorn, the fruit of Cherokee rose, Chinese gall, aleppo avens, wood indigo plant, pilose gerbera herb, Virgin's milk mushroom, galangal, garlic, nutmeg, sea-buckthorn, rhizoma atractylodis, Radix Glycyrrhizae, sealwort, the stem of noble dendrobium, the fruit of glossy privet, giant knotweed, Ligusticum wallichii, west safflower, suchow mosla herb, storax, golden cypress and the banksia rose.
Further, described Chinese herbal feed additive only consists of above-mentioned raw materials medicine.
In described Chinese herbal feed additive, the weight portion of each bulk drug is respectively the Radix Astragali 55 weight portion~65 weight portions, the bighead atractylodes rhizome 35 weight portion~45 weight portions, kuh-seng 20 weight portion~30 weight portions, the membrane of a chicken's gizzard 20 weight portion~30 weight portions, hawthorn 35 weight portion~45 weight portions, the fruit of Cherokee rose 25 weight portion~35 weight portions, Chinese gall 30 weight portion~40 weight portions, aleppo avens 45 weight portion~55 weight portions, blue 20 weight portion~30 weight portions of wood, pilose gerbera herb 25 weight portion~35 weight portions, Virgin's milk mushroom 30 weight portion~40 weight portions, galangal 35 weight portion~45 weight portions, garlic 25 weight portion~35 weight portions, nutmeg 30 weight portion~40 weight portions, sea-buckthorn 25 weight portion~35 weight portions, rhizoma atractylodis 20 weight portion~30 weight portions, Radix Glycyrrhizae 25 weight portion~35 weight portions, sealwort 30 weight portion~40 weight portions, the stem of noble dendrobium 25 weight portion~35 weight portions, the fruit of glossy privet 30 weight portion~40 weight portions, giant knotweed 35 weight portion~45 weight portions, Ligusticum wallichii 20 weight portion~30 weight portions, west safflower 25 weight portion~35 weight portions, suchow mosla herb 30 weight portion~40 weight portions, storax 25 weight portion~35 weight portions, golden cypress 45 weight portion~55 weight portions and the banksia rose 25 weight portion~35 weight portions.
Further preferably, in described Chinese herbal feed additive, the weight portion of each bulk drug is respectively the Radix Astragali 58 weight portion~62 weight portions, the bighead atractylodes rhizome 38 weight portion~42 weight portions, kuh-seng 23 weight portion~27 weight portions, the membrane of a chicken's gizzard 23 weight portion~27 weight portions, hawthorn 38 weight portion~42 weight portions, the fruit of Cherokee rose 28 weight portion~32 weight portions, Chinese gall 33 weight portion~37 weight portions, aleppo avens 48 weight portion~52 weight portions, blue 23 weight portion~27 weight portions of wood, pilose gerbera herb 28 weight portion~32 weight portions, Virgin's milk mushroom 33 weight portion~37 weight portions, galangal 38 weight portion~42 weight portions, garlic 28 weight portion~32 weight portions, nutmeg 33 weight portion~37 weight portions, sea-buckthorn 28 weight portion~32 weight portions, rhizoma atractylodis 23 weight portion~27 weight portions, Radix Glycyrrhizae 28 weight portion~32 weight portions, sealwort 33 weight portion~37 weight portions, the stem of noble dendrobium 28 weight portion~32 weight portions, the fruit of glossy privet 33 weight portion~37 weight portions, giant knotweed 38 weight portion~42 weight portions, Ligusticum wallichii 23 weight portion~27 weight portions, west safflower 28 weight portion~32 weight portions, suchow mosla herb 33 weight portion~37 weight portions, storax 28 weight portion~32 weight portions, golden cypress 48 weight portion~52 weight portions and the banksia rose 28 weight portion~32 weight portions.
Most preferably, in described Chinese herbal feed additive, the weight portion of each bulk drug is respectively the Radix Astragali 60 weight portions, the bighead atractylodes rhizome 40 weight portions, kuh-seng 25 weight portions, the membrane of a chicken's gizzard 25 weight portions, hawthorn 40 weight portions, the fruit of Cherokee rose 30 weight portions, Chinese gall 35 weight portions, aleppo avens 50 weight portions, blue 25 weight portions of wood, pilose gerbera herb 30 weight portions, Virgin's milk mushroom 35 weight portions, galangal 40 weight portions, garlic 30 weight portions, nutmeg 35 weight portions, sea-buckthorn 30 weight portions, rhizoma atractylodis 25 weight portions, Radix Glycyrrhizae 30 weight portions, sealwort 35 weight portions, the stem of noble dendrobium 30 weight portions, the fruit of glossy privet 35 weight portions, giant knotweed 40 weight portions, Ligusticum wallichii 25 weight portions, west safflower 30 weight portions, suchow mosla herb 35 weight portions, storax 30 weight portions, golden cypress 50 weight portions and the banksia rose 30 weight portions.
The present invention also provides the preparation method of above-mentioned Chinese herbal feed additive, is specially:
Above-mentioned raw materials medicine component is mixed by weight, obtain mixture, put into reactor, add the water with respect to 3~5 times of mixture qualities, slow fire heating decocts 2~4 hours, and decoction liquor is filtered, filter residue adds the water with respect to 2~4 times of the mixture qualities obtaining above, and slow fire boiling is 1~3 hour again, and decoction liquor is filtered again, filtered fluid is merged, concentrated except anhydrating, obtain lotion, dry, pulverize, obtain Chinese herbal feed additive.
Each bulk drug pharmacology is as follows:
The Radix Astragali: latin name Astragali Radix, taste is sweet, warm in nature, enters spleen, lung channel, there is help spleen soil, elevate a turnable ladder yang-energy, strengthening exterior and reducing sweat, the effect of holder sore myogenic, cures mainly the weak anorexia and loose stool due to spleen-lung Qi deficiency, palpitation, the visceral organ ptosis of the sinking of qi of middle-jiao, Qi dysfunction in blood control uterine bleeding is had blood in stool, and rushes down for a long time prolapse of the anus; The spontaneous sweating of exterior deficiency, the cards such as insufficiency of vital energy and blood.The Radix Astragali strengthens heart contraction ability, can improve the blood circulation of whole body, can coronary artery dilator, kidney blood vessel and adder-wort peripheral vessel, make skin blood overactive circulation; Can improve the nonspecific immunity power of body, protect the liver and fall hypoglycemic effect, staphylococcus, blood dissolubility streptococcus, Diplococcus pneumopniae, shigella dysenteriae etc. are had to inhibitory action.
The bighead atractylodes rhizome: taste is sweet, bitter, warm in nature, enters spleen, stomach warp, has tonifying spleen profit gas, eliminating dampness Li Shui, and strengthening exterior and reducing sweat, the effect that fortune spleen is moisturized, is mainly used in ruffian due to the dysfunction of the spleen in transport, QI failing to transform into body fluid and has one's mind filled with and rush down, phlegm and retained fluid oedema; The vexed lazy speech of the deficiency of qi in middle-jiao, metrorrhagia and metrostaxis, proctoptosis with chronic dysentery; The cards such as solid in the playing of deficiency of the spleen causing weakness of QI, abnormal sweating spontaneous perspiration.
Kuh-seng: latin name Sophorae Flavescentis Radix, bitter, cold in nature, enter the heart, stomach, large intestine, urinary bladder channel, there is the effect of heat-clearing and damp-drying drug, wind dispelling insecticide, be mainly used in damp-heat dysentery, jaundice, stranguria, kuh-seng has obvious inhibitory action to shigella dysenteriae, Escherichia coli, proteus, beta streptococcus and staphylococcus aureus.
The membrane of a chicken's gizzard: taste is sweet, cold in nature, return spleen, stomach, small intestine, urinary bladder channel, there is reinforcing spleen to promote digestion, puckery essence is stopped excessive effect, cures mainly dyspepsia turgor, vomiting gastric disorder causing nausea, rushes down dysentery, and infantile malnutrition due to digestive disturbances or intestinalparasites, quenches one's thirst, and loses and drowns, larynx numbness acute tonsillitis, the cards such as noma aphtha.
Hawthorn: latin name Crataegi Fructus, taste acid, sweet, slightly warm in nature, returns spleen, stomach, Liver Channel, for food digesting stomach fortifying, the promoting the circulation of qi stasis of blood of faling apart, is mainly used to treat accumulation of food in the stomach and intes tine due to indigestion, has loose bowels, dysentery, hawthorn is the staple for the treatment of indigestion, hawthorn can increase the secretion of peptic digest enzyme, promotes digestion; Shigella dysenteriae, Escherichia coli, Pseudomonas aeruginosa, staphylococcus aureus etc. are had to obvious inhibitory action.
The fruit of Cherokee rose: latin name Rosae Laevigatae Fructus, taste acid, sweet, property is puckery, flat, returns kidney, bladder, large intestine channel, has controlling nocturnal emission with astringent drugs contracting urine, curing metrorrhagia and leukorrhagia, the effect of relieving diarrhea with astringents, cures mainly emission and spermatorrhea, and enuresis frequent micturition, under uterine bleeding band, is rushed down the cards such as protracted dysentery for a long time.
Chinese gall: latin name Galla Chinensis, taste acid, puckery, cold in nature, return lung, large intestine, kidney channel, have that convergence is astringent or styptic treatment for spontaneous sweating, an effect of nourishing generate fluid, kidney calming, cure mainly: chronic cough of deficiency lung; Spontaneous sweating; Protracted dysentery is rushed down for a long time; Prolapse of the anus; Seminal emission; Gonorrhoea; Various hemorrhage; The cards such as the swollen sore furuncle of carbuncle, Chinese gall can strengthen the defence capability of body to nonspecific stimulation, can strengthen the immunologic function of cell, and this pathogenic bacteria are had to inhibitory action, and can antivirally grow.
Aleppo avens: latin name Gei Herba, taste is pungent, bitter, and property is flat, returns liver, spleen, lung channel, has analgesia, step-down, menstruation regulating, the effect of dispelling rheumatism, cures mainly high blood pressure, dizziness headache, irregular menstruation, the pain of lower abdomen, leukorrhea, child convulsion, lumbago, skelalgia of rheumatism; Carbuncle furuncle poison, the cards such as traumatic injury are controlled in external application.
Wood is blue: mildly bitter flavor, and cold in nature, have clearing heat and detoxicatingly, the effect of cooling blood and hemostasis, cures mainly the diseases such as encephalitis B, mumps, acute laryngopharyngitis, lymphnoditis, hot eyes, aphtha, erysipelas, worm snake bite, haematemesis.
Pilose gerbera herb: bitter, pungent, property is flat, has the cough-relieving of a surname's lung, clearing heat and detoxicating, dampness removing, the effect of the promoting flow of qi and blood circulation, cures mainly cough due to common cold, and asthma is had loose bowels, dysentery, oedema, stranguria with turbid discharge, epigastric distending pain, treating swelling and pain by traumatic injury, the cards such as venomous snake bite.
Virgin's milk mushroom: bitter, pungent, warm in nature, there is expelling wind and clearing away cold, the effect of stimulating the circulation of the blood and cause the muscles and joints to relax, cures mainly lumbocrural pain, numb in every limb, and muscles and bones is not relaxed, the cards such as tic of limbs.
Galangal: taste is pungent, hot in nature, return spleen, stomach warp, there is warming stomach for dispelling cold, the effect of the pain relieving that helps digestion.For coldness and pain in the epigastrium, gastrofrigid vomiting, the cards such as belch acid regurgitation.
Garlic: warm in nature, taste is pungent flat; Enter spleen, stomach, lung channel, have in temperature help digestion, the effect of row stagnant gas, warming spleen and stomach, long-pending, the removing toxic substances of disappearing, desinsection, cure mainly eating accumulation, coldness and pain in the epigastrium, oedema turgor, have loose bowels, the illness such as dysentery, malaria, pertussis, swollen ulcer drug, white bald tinea sore, snake bite and insect sting and hookworm, pinworm.
Nutmeg: latin name Myristicae Semen, taste is pungent, bitter, warm in nature, enters spleen, stomach, large intestine channel, has the effect of puckery intestines, promote qi circulation digestion promoting in temperature, for void rush down, the card such as cold dysentery, abdominal distention, food are vomitted less, place food does not disappear.
Sea-buckthorn: taste acid; Puckery; Warm in nature, have relieving cough and reducing sputum; Stomach strengthening and digestion promoting; The effect of promoting blood circulation to remove blood stasis.Cure mainly coughing with a lot of sputum; Pulmonary abscess; Indigestion; Dyspeptic abdominalgia; Stomachache; Enteritis; Amenorrhoea; Fall and beat the swollen card that waits of the stasis of blood.
Rhizoma atractylodis: taste is pungent, bitter, warm in nature, enter spleen, stomach, Liver Channel, have drying damp and strengthening spleen, expelling wind and eliminating dampness, the effect of improving eyesight, is mainly used in the bellyache and diarrhea dysentery of damp retention in middle-jiao, incoordination between the spleen and the stomach abdominal distension pain, vomits indigestion and loss of appetite; The abdomen side of body distending pain of liver-stomach disharmony, vomiting with acid regurgitation; And the cards such as wind-cold-dampness arthralgia of the outer strongly fragrant channels and collaterals of cold-dampness, rhizoma atractylodis are higher to the clearance rate of the colibacillary R-plasmid of chicken characteristic of disease, thereby can eliminate the colibacillary drug resistance of plasmid antagonism of antibody-resistant bacterium.
Radix Glycyrrhizae: taste is sweet, property is flat, enters 12 warps, has clearing heat and detoxicatingly, is in harmonious proportion the property of medicine, the effect of expelling phlegm and arresting coughing, and can invigorating the spleen and in, relieving spasm to stop pain, is mainly used in weakness of the spleen and the stomach abdominal distension and anorexia, weak; Can alleviate contraction pain in abdomen, relax the strong and toxicity of some drugs, alleviate medicine to the toxic and side effect of body or to GI stimulation, by adding Radix Glycyrrhizae can play tonifying Qi, neutralize, be in harmonious proportion the effect of each component.
Sealwort: taste is sweet, property is flat, enters lung, spleen, kidney channel, has nourshing kidney moistening lung, the effect of invigorating the spleen and benefiting qi, for the lung-dryness syndrome due to yin deficiency, dry cough phlegm is few, and many drinks of quenching one's thirst are weakness of the spleen and the stomach, Spleen-Qi Deficiency or deficiency of spleen-YIN, deficiency of kidney essence, soreness and weakness of waist and knees, the cards such as poliosis.
The stem of noble dendrobium: cold nature, taste is sweet, enters stomach, kidney channel, has reinforcing stomach reg fluid, nourishing Yin and clearing heat effect, for heat consuming body fluid, low-heat polydipsia, the red few tongue of tongue; Deficiency of stomach-Yin, thirsty and dry pharynx, the contrary few food of vomitting, vague epigastralgia, the few tongue of tongue light; The deficiency of the kidney yin, the diseases such as blurring of vision.
The fruit of glossy privet: taste is sweet, bitter, is slightly cold, enters liver, kidney channel, has strong liver kidney-nourishing, and the effect of removing heat to brighten vision, is mainly used in treating liver-kidney Yin deficiency, and the head loses foster dizzy tinnitus, the symptoms such as yin asthenia generating intrinsic heat.The fruit of glossy privet can promote leucocyte, strengthens the effect of immunologic function.
Giant knotweed: mildly bitter flavor, cold nature, returns liver, courage, lung channel, have clearing heat and detoxicating, normalizing gallbladder to cure jaundice, expelling wind and removing dampness, loose stasis of blood analgesic therapy, relieving cough and reducing sputum effect, for joint pain, jaundice with damp-heat pathogen, through closing wei lump in the abdomen, coughing with a lot of sputum, scald, injury from falling down, the cards such as carbuncle sore tumefacting virus.
Ligusticum wallichii: taste is pungent, warm in nature, return liver, courage, pericardium channel, there is blood-activating and qi-promoting, the effect of wind-expelling pain-stopping, cures mainly irregular menstruation; Through closing dysmenorrhoea; The stagnant raw meat pain of the stasis of blood in postpartum; Abdominal mass lump; Pain in chest and hypochondrium; Have a headache dizzy; Wind-cold-dampness arthralgia; Traumatic injury; The cards such as ulcer sores.
West safflower: taste is sweet, property is flat, can be promoting blood circulation and removing blood stasis, loose Yu Kaijie, pain relieving.Be used for the treatment of troubled thoughts pent-up, chest diaphragm ruffian is vexed, spits blood, and typhoid fever is gone mad, and is frightenedly dimly afraid of, and women is through closing, stasis irregular menstruation, and prolonged lochia, hemostasis is had a pain, measles, the cards such as traumatic injury.
Suchow mosla herb: warm in nature, taste is pungent, has dispelling summer-heat to relieve superficies syndrome, the effect of regulating qi-flowing for relieving pain, cures mainly flu, heatstroke, eruptive disease, peratodynia, the cards such as abscess of throat.
Storax: taste is pungent, micro-sweet, bitter, warm in nature, the thoughts of returning home, the spleen channel, having has one's ideas straightened out wards off dirtyly, opens strongly fragrant open-minded phlegm, and the effect of promoting qi circulation and relieving pain, cures mainly apoplexy, coma due to blocking of the respiratory system, the cold card that closes of losing consciousness, the wet turbid cards such as profit of telling.
Golden cypress: bitter, cold in nature, enter kidney, bladder, large intestine channel, heat-clearing and damp-drying drug, purging intense heat and detonicating, except hectic fever due to yin clearind deficient heat.For damp-heat dysentery, jaundice, under band, heat is drenched, beriberi, and impotence is warded off, hectic fever due to yin labor heat, night sweat, seminal emission, sore swollen toxin, eczema itch.
The banksia rose: taste is pungent, hardship, warm in nature, return spleen, stomach, liver, lung channel, has promoting qi circulation and relieving pain, the effect of reinforcing spleen to promote digestion, cure mainly the chest side of body, play abdominal distention and pain, rush down after dysentery heavy, the card such as dyspepsia does not disappear, do not feel like eating.
The present invention also provides the preparation method of above-mentioned feed, and it comprises:
The first step, get 3kg microbe additive and join in 100kg~120kg water and mix, obtain zymotic fluid, the zymotic fluid that 100kg is obtained joins in 100kg main feed composition, evenly pack sealing, is placed in the environment of 20 ℃~30 ℃ and obtains fermentation material after 10~20 days;
Second step, prepares Chinese herbal feed additive according to the method described above;
The 3rd step, together with the Chinese herbal feed additive that the fermentation material that the first step is obtained obtains with second step and other components, put into fodder mixing machine, continuous uniform stirred after 1~3 hour, putting into pulverizer tentatively pulverizes, after taking-up, putting into micronizer, to carry out separating twice be fine grained again, the feed that gets product after sterilization, sealing, pack of the fine grained after pulverizing.
Below adopt embodiment to describe embodiments of the present invention in detail, to the present invention, how application technology means solve technical problem whereby, and the implementation procedure of reaching technique effect can fully understand and implement according to this.
Embodiment 1 Chinese medicine composition feed addictive 1
By Radix Astragali 60g, bighead atractylodes rhizome 40g, kuh-seng 25g, the membrane of a chicken's gizzard 25g, hawthorn 40g, fruit of Cherokee rose 30g, Chinese gall 35g, aleppo avens 50g, the blue 25g of wood, pilose gerbera herb 30g, Virgin's milk mushroom 35g, galangal 40g, garlic 30g, nutmeg 35g, sea-buckthorn 30g, rhizoma atractylodis 25g, Radix Glycyrrhizae 30g, sealwort 35g, stem of noble dendrobium 30g, fruit of glossy privet 35g, giant knotweed 40g, Ligusticum wallichii 25g, west safflower 30g, suchow mosla herb 35g, storax 30g, golden cypress 50g and banksia rose 30g mix, obtain mixture, put into reactor, the water that adds 3.8kg, slow fire heating decocts 3 hours, decoction liquor is filtered, filter residue adds the water of 2.8kg, slow fire boiling is 2 hours again, decoction liquor is filtered again, filtered fluid is merged, concentrated except anhydrating, obtain lotion, dry, pulverize, obtain Chinese herbal feed additive 1.
Embodiment 2 Chinese medicine composition feed addictives 2
By Radix Astragali 58g, bighead atractylodes rhizome 38g, kuh-seng 23g, the membrane of a chicken's gizzard 23g, hawthorn 38g, fruit of Cherokee rose 28g, Chinese gall 33g, aleppo avens 48g, the blue 23g of wood, pilose gerbera herb 28g, Virgin's milk mushroom 33g, galangal 38g, garlic 28g, nutmeg 33g, sea-buckthorn 28g, rhizoma atractylodis 23g, Radix Glycyrrhizae 28g, sealwort 33g, stem of noble dendrobium 28g, fruit of glossy privet 33g, giant knotweed 38g, Ligusticum wallichii 23g, west safflower 28g, suchow mosla herb 33g, storax 28g, golden cypress 48g and banksia rose 28g mix, obtain mixture, put into reactor, the water that adds 3.2kg, slow fire heating decocts 3 hours, decoction liquor is filtered, filter residue adds the water of 2.5kg, slow fire boiling is 2 hours again, decoction liquor is filtered again, filtered fluid is merged, concentrated except anhydrating, obtain lotion, dry, pulverize, obtain Chinese herbal feed additive 2.
Embodiment 3 Chinese medicine composition feed addictives 3
By Radix Astragali 62g, bighead atractylodes rhizome 42g, kuh-seng 27g, the membrane of a chicken's gizzard 27g, hawthorn 42g, fruit of Cherokee rose 32g, Chinese gall 37g, aleppo avens 52g, the blue 27g of wood, pilose gerbera herb 32g, Virgin's milk mushroom 37g, galangal 42g, garlic 32g, nutmeg 37g, sea-buckthorn 32g, rhizoma atractylodis 27g, Radix Glycyrrhizae 32g, sealwort 37g, stem of noble dendrobium 32g, fruit of glossy privet 37g, giant knotweed 42g, Ligusticum wallichii 27g, west safflower 32g, suchow mosla herb 37g, storax 32g, golden cypress 52g and banksia rose 32g mix, obtain mixture, put into reactor, the water that adds 4kg, slow fire heating decocts 3 hours, decoction liquor is filtered, filter residue adds the water of 3kg, slow fire boiling is 2 hours again, decoction liquor is filtered again, filtered fluid is merged, concentrated except anhydrating, obtain lotion, dry, pulverize, obtain Chinese herbal feed additive 3.
Embodiment 4 pharmacology toxicity tests
Acute toxicity test data
Detect reagent
Serum AST, ALT detection kit are purchased from Rui Sheng bio tech ltd, Qingdao.
Checkout equipment
Micro-200 semiautomatic biochemistry detector, assay balance, disscting instrument, syringe, test tube etc.
Test method
After the adaptability of 3 days are raised, be divided at random 4 groups (male and female half and half), every group each 20; Control group, 3 different pharmaceutical groups (are respectively 300g/kg body weight, 240g/kg body weight, 180g/kg body weight), control group gavage physiological saline, the Chinese herbal feed additives 1 of 3 medicine group gavage embodiment of the present invention, 1 preparation, are dissolved in Chinese herbal feed additive in water, to gavage mouse.
Testing index and method
(1) clinicing symptom observation: every day viewing test mouse the state of mind, diet situation, every day entry Mortality situation.
(2) pathological observation: cut open inspection for dead mouse and observe internal organ variation.
(3) growth indexes is observed: test mouse is carried out to routine weighing; Off-test, slaughters that mouse weighs in, the heavy organ index of internal organs measures: cut open inspection rat, dirty, the spleen of coring, liver, kidney, testis, ovary, weigh, and calculates according to the following formula organ index: organ index=internal organs weight/body weight * 100.
(4) liver function changes observation: after off-test, slaughter mouse, take blood separation of serum, measure AST, ALT.
Data processing and analysis
Each is organized data and all uses mean soil standard deviation
represent, each is organized mean and does test of normality and duplicate measurements homogeneity test of variance, younger check between organizing with one-way analysis of variance), P<0.05 is for differing significantly, and P<0.01 is for differing highly significant.Statistical computation all adopts SPSS12.0 software to complete.
Clinicing symptom observation
Control group and test group mouse, the state of mind and diet are wanted, respond has no significantly abnormal to external world.Each organizes mouse morbidity and death condition as following table 1.
Table 1
Grouping |
Sum |
Dead (only) |
The death rate (%) |
1(300g/kg) |
20 |
0 |
0 |
2(240g/kg) |
20 |
0 |
0 |
3(120g/kg) |
20 |
0 |
0 |
Control group |
20 |
0 |
0 |
Pathological observation: control group and test group, mouse is cutd open after slaughtering to examine to observe to have no and observes the obvious pathological change of internal organs.
Growth indexes is observed: off-test, slaughter that mouse weighs in, internal organs weight analysis is in Table 2.
Table 2
Grouping |
Liver index |
Cardiac index |
Renal index |
Lungs index |
Index and spleen index |
1(300g/kg) |
5.67±0.31 |
0.81±0.03 |
1.26±0.21 |
0.68±0.06 |
0.49±0.05 |
2(240g/kg) |
5.73±0.25 |
0.76±0.05 |
1.27±0.22 |
0.71±0.08 |
0.55±0.09 |
3(120g/kg) |
5.77±0.21 |
0.78±0.09 |
1.23±0.17 |
0.75±0.09 |
0.51±0.05 |
Control group |
5.70±0.31 |
0.79±0.06 |
1.30±0.17 |
0.69±0.11 |
0.53±0.07 |
Liver function changes to be observed
After off-test, slaughter mouse, take blood separation of serum, measure AST (glutamic-oxalacetic transaminease), ALT (glutamic-pyruvic transaminase) in Table 3.Result shows respectively to be organized data and normal healthy controls group to compare difference not remarkable, all in normal range (NR).
Table 3
Grouping |
Sum |
ALT |
AST |
1(300g/kg) |
20 |
32.93±3.17 |
133.62±8.51 |
2(240g/kg) |
20 |
33.92±2.91 |
131.92±10.27 |
3(120g/kg) |
20 |
33.72±2.73 |
130.62±10.17 |
Control group |
20 |
35.11±3.26 |
131.26±14.11 |
Long term toxicity test data
According to the Chinese herbal feed additive 10g/kg of the present invention of embodiment 1 preparation, 20g/kg, 100g/kg to the continuous gastric infusion of rat 3 months, the fur of animal, the colour of skin, ingest, movable, urine, just etc. all without the main organs index of the blood parameters such as the routine blood test of animal extremely, liver, renal function and animal all in normal range (NR), and normally power indifference histopathologic examination shows, the important organs such as the heart, liver, spleen, lung, kidney, adrenal gland, tracheae, testis, ovary are without pathological change.Drug withdrawal checks after two weeks that above indices, all without overt toxicity reaction, shows without cumulative toxicity.
Embodiment 5 feeds 1
Getting 3kg microbe additive source health precious (Shandong Liuhe Farming Science and Technology Park Co., Ltd provides) joins in 100kg water and mixes, obtain zymotic fluid, the zymotic fluid that 100kg is obtained joins 80kg corn, in the main feed composition that 5kg wheat bran and 15kg dregs of beans form, evenly pack sealing, be placed in the environment of 25 ℃ and obtain fermentation material after 15 days, by Chinese herbal feed additive 16kg and galactomannan (rural area, the Zhengzhou food science and technology Co., Ltd provides) 3kg of the fermentation material obtaining and embodiment 1 acquisition, betaine (Qingdao Lv Cheng Co., Ltd provides) 7kg, nicotinate chromium (in Beijing, source, Haikang Science and Technology Ltd. provides) 2kg, soybean lecithin (source, Beijing magnificent phosphatide Science and Technology Ltd. provides) 3kg, Bee Pollen (Guangzhou Tan Shan apiculture Co., Ltd provides) 4kg, salt 2kg, bone meal 4kg, sodium bicarbonate 2kg, alfalfa meal (Jiahe, Qingdao feed corporation,Ltd provides) 6kg, amino acid additive composite aminophenol powder (above sea cowry energy Chemical Co., Ltd. produces) 5kg, the excellent dimension element of vitamin(e) supplement (production of Beijing You Libao Bioisystech Co., Ltd) 5kg puts into fodder mixing machine together, continuous uniform stirred after 3 hours, putting into pulverizer tentatively pulverizes, after taking-up, putting into micronizer, to carry out separating twice be fine grained again, fine grained after pulverizing is through sterilization, sealing, feed gets product after pack.
Comparative example feed 2
Get 3kg microbe additive source health precious (Shandong Liuhe Farming Science and Technology Park Co., Ltd provides) and join in 100kg water and mix, obtain zymotic fluid, the zymotic fluid that 100kg is obtained joins 80kg corn, in the main feed composition that 5kg wheat bran and 15kg dregs of beans form, evenly pack sealing, is placed in the environment of 25 ℃ and obtains fermentation material after 15 days, by the fermentation material and galactomannan (rural area, the Zhengzhou food science and technology Co., Ltd provides) 3kg that obtain, betaine (Qingdao Lv Cheng Co., Ltd provides) 7kg, nicotinate chromium (in Beijing, source, Haikang Science and Technology Ltd. provides) 2kg, soybean lecithin (source, Beijing magnificent phosphatide Science and Technology Ltd. provides) 3kg, Bee Pollen (Guangzhou Tan Shan apiculture Co., Ltd provides) 4kg, salt 2kg, bone meal 4kg, sodium bicarbonate 2kg, alfalfa meal (Jiahe, Qingdao feed corporation,Ltd provides) 6kg, amino acid additive composite aminophenol powder (above sea cowry energy Chemical Co., Ltd. produces) 5kg, the excellent dimension element of vitamin(e) supplement (production of Beijing You Libao Bioisystech Co., Ltd) 5kg puts into fodder mixing machine together, continuous uniform stirred after 3 hours, putting into pulverizer tentatively pulverizes, after taking-up, putting into micronizer, to carry out separating twice be fine grained again, and the fine grained after pulverizing is through sterilization, sealing, feed 2 gets product after pack.
Embodiment 6 effect datas
Test feed
Test group adopts the feed 1 of embodiment 5 preparations, the feed 2 that control group adopts comparative example to be prepared into.
Experimental animal and feeding and management
Select 20 ages in days to physically well develop, active healthy yellow-feather broiler (hen) ,You Qingdao City Shun Qing Industrial Co., Ltd. experiment chicken house provides.
Test by Stages: middle chicken (21~45 age in days), large chicken (46~78 age in days), test finishes since 21 age in days to 78 ages in days.
Test is raised: Manual material feeding, free choice feeding, freely drinks water.Stocking density is with reference to the feeding and management requirement of test broiler chicken kind.The temperature of hen house, humidity and illumination are determined according to routine.According to the existing immune programme for children of chicken house, carry out, during morbidity, according to conventional treatment.Carry out the record of humiture, chicken morbidity, death and prevention and the medicine for treatment of hen house every day.
Test item and method
1d after off-test, often repeats to choose 1 of the chicken that approaches average weight, and neck bloodletting is butchered, fasting 12h before killing.Get left side chest muscle sample for measuring muscle moisture, thick protein, crude fat, yellowish pink, tender degree.
Moisture
Get and a certain amount ofly smash rear meat sample to pieces and be weighed as A, put into moisture ware and weigh (W), in baking oven, dry after 4h for 250 ℃, insert the cooling 30min of drying basin inner drying, be weighed as B, being calculated as follows is waterpower: moisture=(W-B/A) * 100%.
Thick protein
With tissue mashing machine, smash meat sample to pieces, constant temperature drying, takes 2g and is put in digestion tube, adds antioxidant 2.5g and concentrated sulfuric acid 10mL, is placed in and on High-temperature Digestion stove, carries out Specimen eliminating.According to Kai Shi semimicro nitriding, application kjeldahl apparatus is measured, reduction formula:
Crude protein %=(U-U
0) * 0.014 * 6.25 * 0.1612/W * 100%
In formula, U
0for blank, W is sample heavy (g), and 0.1612 is sulfuric acid concentration, and U is machine-readable number.
Crude fat
Rou Yangyong tissue mashing machine smashs to pieces and mixes, and constant temperature drying takes 1g sample and is placed in filtration paper cylinder; Filtration paper cylinder weighs after carrying out drying for 2 times until constant weight is applied Milko-Tester and measured crude fat.
Yellowish pink
Get green meat sample 25g (treading on and fat without muscle), add immediately water 50mL, in tissue mashing machine, smash making beating to pieces, full dose put into 50mL centrifuge tube carry out the 1st time centrifugal, taking out centrifuge tube is respectively charged into supernatant in 2~3 little centrifuge tubes, to carry out the 2nd time centrifugation (3000r/min, 10min) after taking-up centrifuge tube, with filter paper, the fat of liquid level is removed, supernatant is placed in to cuvette (optical path 1cm), with spectrophotometer, at 540nm wavelength place, measure OD value.
Tender degree
Take 10g meat sample and put into 100mL stainless steel homogenate cup, add cold sucrose (0.24mol/L) simultaneously, potassium chloride (0.2mol/L) equivalent mixed liquor 50mL, after standing 5min, sample stirs at full speed and cuts 40s with refiner.With the aperture of a known weight, be the strainer filtering of 250 μ m, filter screen and residue blot and weigh with blotting paper.Then calculating can not be by broken section of weight of meat sample (being greater than 250 μ m) of filter screen, is converted into the shatter index (FI) that contained residue weight in every 100g meat sample is muscle.FI value is less, shows that meat is more delicate.
Muscle drip loss
1 (W that weighs of sampling position
1) be placed in sealing polybag, put into immediately 4 ℃ of refrigerators, after 24h, with filter paper, wipe meat surface moisture, the 2nd (W that weighs
2).
Muscle drip loss (%)=(W
1-W
2)/W
1* 100%
Cholesterol (Cholester0l) assay: take chest muscle 0.1g, add 5ml chloroform: methyl alcohol=2:1 solution, homogenate.The centrifugal 10min of homogenate 3000r/min, gets supernatant evaporate to dryness under 65 ℃ of water-baths, then with the kit that Ningbo Ci Cheng biochemical reagents length provides, measures cholesterol level on ultraviolet-visible spectrophotometer.
Inosinicacid (Ino sinic acid) assay: sample accurately takes 1g, put in 10mL glass homogenizer, with 5mL3.5% perchloric acid solution, divide 3 homogenate, homogenate proceeds in centrifuge tube, and washs homogenizer with a small amount of 3.5% perchloric acid, and washing lotion is incorporated in centrifuge tube, with the centrifugal 10min of 3500r/min, draw supernatant, sediment with 1.5mL3.5% perchloric acid solution wash again, centrifugal, merge twice supernatant as extract.With 0.5mol/1NaOH solution, adjust extract to p H=6.5, water is settled to 50mL, shakes up, and crosses 0.22 μ m filter membrane.Splitter, after mobile phase balance 1h, is got chromatographic column on each 20 μ L of standard series working solution and sample extracting solution.
With agilent1100 efficient liquid phase chromatographic analysis instrument (HCLP), Alltech C18 splitter (4.6 * 250mm, 5 μ m), eluent flow 1mi/min at 25 ℃, recorder low speed 0.5cm/min, detector wavelength is 254nm, and staking-out work curve, measures imp content by peak area quantification.
Data processing and analysis
Each is organized data and all uses mean soil standard deviation
represent, each is organized mean and does test of normality and duplicate measurements homogeneity test of variance, younger check between organizing with one-way analysis of variance), P<0.05 is for differing significantly, and P<0.01 is for differing highly significant.Statistical computation all adopts SPSS12.0 software to complete.
Result
The impact of Chinese herbal feed additive on yellow-feather broiler meat physicochemical property, in Table 4.
The impact of table 4 Chinese herbal feed additive yellow-feather broiler meat physicochemical property
Project |
Control group |
Test group |
Yellowish pink |
1.27±0.09 |
1.68±0.07 |
Tender degree (g/100g) |
18.29±0.14 |
21.35±0.29 |
Drip loss (%) |
2.11±0.31 |
1.87±0.29 |
Yellowish pink is the important indicator of muscle outward appearance evaluation, and yellowish pink is that embody the outside that physiology, biochemistry and the microbiology of muscle changes, and directly affects consumer's purchase decision.The yellowish pink impact that is mainly subject to myoglobins state, is secondly also subject to the impact of light reflection, oxidation.Myoglobin content has a certain impact to yellowish pink, and more fleshiness look darker for content.But determine that yellowish pink key is the chemical characteristic of myoglobins.The colour generation effect of myoglobins comes from its intramolecular heme.Heme has very strong affinity to oxygen, and when it is not when oxygen is combined, myoglobins is kermesinus; After being combined with oxygen, myoglobins is cerise.If the Fe in heme
2+be oxidized to Fe
3+after, myoglobins becomes metmyoglobin, is crineous, and the different colours that common people observe muscle is relevant with the ratio of oxymyoglobin, myoglobins and 3 kinds of albumen of oxidation myoglobins.As can be seen from Table 4, the yellowish pink of test group is significantly higher than control group, significant difference (P<0.05).
Tender degree is an importance of meat, is the indexs of people to muscle mouthfeel satisfaction, directly affects edibility and the commodity value of meat.It is the reflection of meat internal structure, and has reflected to a certain extent muscle fibril in meat, connective tissue and muscle fat content, distribution and chemical constitution state.Tender degree is subject to various factors, yet in muscle, the content of 3 kinds of protein ingredients such as connective tissue, muscle fibril and sarcoplasm and chemical constitution state are the main matter bases that determines the tender degree of meat.As can be seen from Table 4, the tender degree of test group is significantly higher than control group, significant difference (P<0.05).
Drip loss is the another important indicator of chicken meat quality, is that it is directly connected to quality, local flavor and the structural state of meat for weighing the index of muscular system waterpower.Drip loss is less, and muscle is that waterpower is just larger, and meat is better.Result of the test shows, adding compound Chinese herbal medicinal feed additive has the trend of the drip loss that reduces muscle, percentage of water loss declines, show that moisture is difficult for oozing out, this is very favourable to maintaining the quality of tender degree, succulence and meat products of muscle, as can be seen from Table 4, the tender degree of test group is significantly lower than control group, significant difference (P<0.05).
The impact of Chinese herbal feed additive on yellow-feather broiler muscle conventional nutrient, the results are shown in Table 5.
The impact of table 5 Chinese herbal feed additive on yellow-feather broiler muscle conventional nutrient
Project |
Control group |
Test group |
Moisture (%) |
72.18±3.88 |
72.81±2.91 |
Crude protein (%) |
25.12±2.73 |
31.21±2.11 |
Crude fat (%) |
0.79±0.05 |
0.93±0.06 |
As can be seen from Table 5, the moisture difference in each processed group muscle is remarkable (P>0.05) not, each processed group crude protein and crude fat content significant difference (P<0.05), and test group is apparently higher than control group.
Inosinicacid and cholesterol comparison, the results are shown in Table 6.
The comparison of table 6 inosinicacid and cholesterol
Project |
Control group |
Test group |
Inosinicacid (%) |
2.17±0.31 |
2.53±0.43 |
Cholesterol (%) |
6.17±2.31 |
9.97±1.93 |
All above-mentioned these intellectual properties of primary enforcement, do not set restriction this new product of other forms of enforcement and/or new method.Those skilled in the art will utilize this important information, and foregoing is revised, to realize similar implementation status.But all modifications or transformation belong to the right of reservation based on new product of the present invention.
The above, be only preferred embodiment of the present invention, is not the present invention to be done to the restriction of other form, and any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the equivalent embodiment of equivalent variations.But every technical solution of the present invention content that do not depart from, any simple modification, equivalent variations and the remodeling above embodiment done according to technical spirit of the present invention, still belong to the protection domain of technical solution of the present invention.