CN103585498A - Method for extracting and detecting polyphenol in bamboo shoots - Google Patents
Method for extracting and detecting polyphenol in bamboo shoots Download PDFInfo
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Abstract
The invention discloses a method for extracting and detecting polyphenol in bamboo shoots. The method comprises the following steps: (1) preparing materials, namely, selecting fresh bamboo shoots, freezing in liquid nitrogen, knocking to shell, and preserving the bamboo shoot meat at minus 70 DEG C for later use; (2) selecting acetone as an extraction solvent, and extracting polyphenol in the bamboo shoots through an ultrasonic low-temperature auxiliary solvent; (3) detecting by using a Folin-ciocalteu method, wherein the detection system comprises 0.25ml of polyphenol extracting solution, 1.75ml of water, 1ml of Folin-ciocalteu color developing agent which is diluted for 6 times and 1ml of 5-15% Na2CO3; the detection condition is that the reaction temperature is 25-35 DEG C in water bath, the reaction time is 0.5-3.5 hours, and the detection wavelength is 755-770nm. Due to adoption of the method, a system for detecting the polyphenol in fresh bamboo shoots and a system for extracting the polyphenol in the bamboo shoots are optimized and established, the detection is rapid and convenient, the extraction efficiency is high, and theoretical foundation can be provided for bamboo shoot development and utilization.
Description
Technical field
The present invention relates to biotechnology, specifically relate to extraction and the detection method of polyphenol in a kind of bamboo sprout.
Background technology
Polyphenol is the compound in plant with the effect of promoting health; Be present in some common plant foods, as cacao bean, tea, Semen sojae atricolor, red wine, vegetable and fruit.Polyphenols has very strong antioxidation.Oxidative damage is to cause many chronic diseases, as cardiovascular diseases, and cancer and old and feeble major reason.The anti-oxidation function of polyphenol can play preventive effect to these chronic diseases.Such as the polyphenol in chocolate is very strong antioxidant, it can suppress the oxidation of low density lipoprotein, LDL (LDL) cholesterol.Polyphenol is except having antioxidation, according in January, 2008 FEDERATION of AMERICAN SOCIETIES for EXPERIMENTAL BIOLOGY (FASEB) magazine, when Israel's research worker is found on the feed high lipid food first, take in polyphenol and can alleviate the threat of high lipid food to health, the derivant that this explanation polyphenol can effectively prevent high lipid food to produce in body metabolism process, as the hazardness of free radical.
Although polyphenol is extensively present in plant food, its active ingredient substance in different plants and structure thereof, content have very big-difference.And the content of polyphenol is subject to many factors in plant, as kind, the course of processing and condition of storage etc.
Bamboo sprout are the tender Seedling of gramineous bamboo department linoleic plant, claim that again bamboo sprouts, bamboo bud.According to one's analysis, the composition such as raw Radix Crotalariae szemoensis rich in proteins, aminoacid, fat, saccharide, calcium, phosphorus, ferrum, carotene and vitamin B1, vitamin B2, vitamin C.Except being rich in multiple nutrients material, bamboo sprout also have higher medical value.The traditional Chinese medical science is thought, bamboo sprout sweet in the mouth, the micro-hardship of part kind are cold in nature, energy eliminating phlegm and keeping the adverse QI downwards, and clearing heat and relieving fidgetness, tonneau two is just; < < Mingyi Bielu > > thinks bamboo sprout " QI invigorating, can eat for a long time for main quenching one's thirst, dredging water passages "; < < A Supplement to the Compendium of Materia Medica > > points out bamboo sprout " saliva that reduces phlegm, helps digestion swollen for sharp nine orifices, promoting blood circulation ", is especially good at Qinghua heat-phlegm.At present, the developmental research about bamboo shoot health effectiveness still belongs to blank; Simultaneously we find that in research repeatedly another kind of important plant health material---flavone content in bamboo sprout is very low, almost examine and do not measure content, so infer that polyphenol is likely the main health substance in bamboo sprout by conventional sense method.In addition, extraction, the detection method about bamboo sprout polyphenol also lacks systematic study report.So we are studied in detail the extraction of bamboo sprout polyphenol and detection method, for the exploitation of bamboo sprout provide theoretical foundation.
Summary of the invention
The technical problem to be solved in the present invention is to lack systematic study for the detection method about bamboo sprout polyphenol in prior art, and extraction and the detection method of polyphenol in a kind of bamboo sprout is provided, and the method extraction efficiency is high, easy to detect.The object of the invention is to: optimize and set up the system of polyphenol content in the new fresh bamboo shoots of detection, and the system of extracting polyphenol in bamboo sprout.
Be compared to existing bamboo sprout polyphenol extracting method, in the present invention, the preservation of bamboo sprout, processing and leaching process all carry out at low temperature, can effectively protect active substance in polyphenol; And directly adopt acetone can effectively improve polyphenol extraction efficiency as extracting solvent; Outside, hyperacoustic utilization can further improve extraction efficiency.
For realizing object of the present invention, the technical solution used in the present invention is as follows:
In extraction and the detection method of polyphenol, steps of the method are:
(1) get the raw materials ready: choose new fresh bamboo shoots, after liquid nitrogen freezing, break into pieces, Radix Crotalariae szemoensis meat is mixed in to-70 ℃ of preservations, standby;
(2) polyphenol in the auxiliary organic solvent extract at low temperature bamboo sprout of ultrasound wave: during extraction, take out a certain amount of bamboo sprout and be placed in mortar, add liquid nitrogen to grind, choose acetone and make to extract solvent, in bamboo sprout, add acetone, after the abundant homogenate of sample, collect in centrifuge tube, in the 15～60min of low temperature supersound extraction below 10 ℃, centrifuging and taking supernatant, obtains bamboo sprout polyphenol extracting solution;
(3) detection of polyphenol in bamboo sprout:
Folin-ciocalteu method detects: in new fresh bamboo shoots, polyphenol detection system is: polyphenol extracting solution 0.25mL, water 1.75mL, dilute Folin-ciocalteu developer 1mL, the 5%～15%Na of 6 times
2cO
31mL;
Testing conditions: reaction temperature: 20～35 ℃ of water-baths, response time: 0.5～3.5h, detects wavelength: 755nm～770nm.
Further, in step (2), choose 30% acetone and make to extract solvent, by 1g bamboo sprout, add 5～30mL30% acetone, after the abundant homogenate of sample, collect in 50mL centrifuge tube, in the 15～60min of low temperature supersound extraction below 10 ℃, centrifuging and taking supernatant, obtains bamboo sprout polyphenol extracting solution.
Further, by 1g bamboo sprout, preferably add 10mL30% acetone to extract.
Further, supersound extraction frequency is 40kHz, ultrasonic power 150W, and extraction time is 45min.
Further, in step (3), Folin-ciocalteu method detects: in new fresh bamboo shoots, polyphenol detection system is: polyphenol extracting solution 0.25mL, water 1.75mL, dilute Folin-ciocalteu developer 1mL, the 15%Na of 6 times
2cO
31mL;
Testing conditions: reaction temperature: 25 ℃ of water-baths, response time: 1.5h, detects wavelength: 755nm.
The present invention has the following advantages:
The invention provides extraction and the detection method of polyphenol in bamboo sprout.The present invention considers that in plant polyphenol, some active component can be oxidized along with the rising of temperature inactivation, all takes K cryogenic treatment, thereby adequately protected the active component in polyphenol in the processes such as bamboo sprout sample preservation, pulverization process and polyphenol extraction; In bamboo sprout preservation process, directly by complete bamboo sprout liquid nitrogen freezing, break afterwards bamboo shell into pieces just effectively separated with Radix Crotalariae szemoensis meat, need not spend specially labour and shell bamboo shell; The method adopts polyphenol in the auxiliary organic solvent extract at low temperature bamboo sprout of ultrasound wave, and usings acetone as extracting solvent, can effectively improve extraction efficiency, and the polyphenol antioxidant activity extracting is stronger; The present invention adopts Folin-Ciocalteu method to detect polyphenol content in bamboo sprout, and has provided detection system and the testing conditions optimized, has advantages of that equipment requirements is low, simple to operate, efficiency is high, precision is high, stability and favorable repeatability; Adopt polyphenol assay method of the present invention can directly measure the polyphenol content of organic solvent extraction, do not need distillation to remove organic solvent, thereby can be used for the large-scale sample of fast detecting.
The specific embodiment
In order to make object of the present invention, technical scheme and advantage clearer, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
The extraction of polyphenol and detection method in the bamboo sprout that the embodiment of the present invention provides, in these bamboo sprout, the extraction of polyphenol and detection method are carried out in accordance with the following steps:
(1) get the raw materials ready: choose new fresh bamboo shoots and shell, after liquid nitrogen freezing, break into pieces, Radix Crotalariae szemoensis meat is mixed in to-70 ℃ of preservations, standby;
(2) polyphenol in the auxiliary organic solvent extract at low temperature bamboo sprout of ultrasound wave: during extraction, take out a certain amount of bamboo sprout and be placed in mortar, add liquid nitrogen to grind, choose acetone and make to extract solvent, in bamboo sprout, add acetone, after the abundant homogenate of sample, collect in centrifuge tube, in the 15～60min of low temperature supersound extraction below 10 ℃, centrifuging and taking supernatant, obtains bamboo sprout polyphenol extracting solution;
(3) detection of polyphenol in bamboo sprout:
Folin-ciocalteu method detects: in new fresh bamboo shoots, polyphenol detection system is: polyphenol extracting solution 0.25mL, water 1.75mL, dilute Folin-ciocalteu developer 1mL, the 5%～15%Na of 6 times
2cO
31mL;
Testing conditions: reaction temperature: 20～35 ℃ of water-baths, response time: 0.5～3.5h, detects wavelength: 755nm～770nm.
A prioritization scheme as the embodiment of the present invention, in step (2), choose 30% acetone and make to extract solvent, by 1g bamboo sprout, add 5～30mL30% acetone, after the abundant homogenate of sample, collect in 50mL centrifuge tube, in the 15～60min of low temperature supersound extraction below 10 ℃, centrifuging and taking supernatant, obtains bamboo sprout polyphenol extracting solution.
As a prioritization scheme of the embodiment of the present invention, by 1g bamboo sprout, preferably add 10mL30% acetone to extract.
As a prioritization scheme of the embodiment of the present invention, supersound extraction frequency is 40kHz, ultrasonic power 150W, and extraction time is 45min.
As a prioritization scheme of the embodiment of the present invention, in step (3), Folin-ciocalteu method detects: in new fresh bamboo shoots, polyphenol detection system is: polyphenol extracting solution 0.25mL, water 1.75mL, dilute Folin-ciocalteu developer 1mL, the 15%Na of 6 times
2cO
31mL;
Testing conditions: reaction temperature: 25 ℃ of water-baths, response time: 1.5h, detects wavelength: 755nm.
Below in conjunction with specific embodiment, application principle of the present invention is further described.
One, optimization and the foundation of polyphenol detection system in new fresh bamboo shoots
Embodiment 1:
The formation of polyphenol detection system comprises: polyphenol extracting solution 0.25mL, water 1.75mL, Folin-ciocalteu developer 1mL, Na
2cO
31mL.Testing conditions comprises reaction temperature, response time, detection wavelength.
The optimization of Na2CO3 concentration and reaction temperature in polyphenol detection system:
The new fresh bamboo shoots of collection Ji Zhu in May and tea stalk bamboo, shreds merging, using acetone as solvent extraction polyphenol is wherein as test material.Folin-ciocalteu developer extension rate is set to 3 times and 4 times, Na
2cO
3concentration is set to 5%, 10% and 15%, and reaction temperature is set to 20 ℃, 25 ℃, 30 ℃, 35 ℃.Experimental results show that and work as Na
2cO
3when concentration is 20% and 30%, after water-bath, form precipitation, so Na
2cO
3in concentration setting, be limited to 15%.
Each response sample was reacted after 1 hour in water-bath, detect the light absorption value of each sample under 765nm wavelength, light absorption value is higher means that detection effect is sensitiveer.The results are shown in Table 1.
The light absorption value of each response sample of table 1 under 765nm wavelength
As can be seen from Table 1, when 3 times of Folin-ciocalteu developer dilutions and 4 times, Na in polyphenol detection system
2cO
3concentration is 15% better, and reaction temperature is better with 25 ℃.
Optimize the extension rate of Folin-ciocalteu developer, simultaneously optimizing reaction time and detection wavelength:
Embodiment 2:
Gather Ji Zhu and tea stalk bamboo bamboo sprout in fresh May, shred, using acetone as solvent extraction polyphenol is wherein as test material.The extension rate of Folin-ciocalteu developer is set to 1 times, 2 times, 3 times, 4 times, 5 times, 6 times, 7 times.Response time is set to 0.5h, 1h, 1.5h, 2h, 2.5h, 3h, 3.5h.Detect wavelength and be set to 755nm, 760nm, 765nm, 770nm.In reaction system with 15%Na
2cO
3for basic component, reaction temperature is 25 ℃ of water-baths.
Each reaction is combined in 25 ℃ of corresponding times of reaction, then in ultraviolet spectrophotometer, measures the light absorption value of 755nm, 760nm, 765nm, 770nm simultaneously.The results are shown in Table 2-table 5.
Detection effect when table 2Folin-ciocalteu developer extension rate is 1 times, 2 times
Detection effect when table 3Folin-ciocalteu developer extension rate is 3 times, 4 times
Detection effect when table 4Folin-ciocalteu developer extension rate is 5 times, 6 times
Detection effect when table 5Folin-ciocalteu developer extension rate is 7 times
From table 2-table 5, can find out that reaction system, 6 times of Folin-ciocalteu developer dilutions are better, when detecting wavelength and being 755nm slightly well; After 25 ℃ of reaction 1.5h of water-bath, light absorption value peaks substantially, and this light absorption value can stably maintain at least 2h.
Conclusion: in new fresh bamboo shoots, polyphenol optimal detection system is: polyphenol extracting solution 0.25mL, water 1.75mL, 6XFolin-ciocalteu developer 1mL, 15%Na
2cO
31mL.Testing conditions comprises that reaction temperature is 25 ℃ of water-baths, response time 1.5h, detection wavelength 755nm.
Two, in new fresh bamboo shoots, polyphenol extracts system optimization and foundation
The optimization that in new fresh bamboo shoots, polyphenol extracts system comprises extracts the selection of solvent, the use amount of solvent (usage ratio), the optimization of extraction time.
The optimization of acetone concentration
Embodiment 3:
The inventor is according to research for many years and through many experiments, find under equal conditions, with the acetone of distilled water, various same concentrations and the polyphenol in ethanol extraction bamboo sprout, the light absorption value of result acetone extract in identical detection system often, higher than the extracting solution of the ethanol of distilled water, same concentrations, illustrates that the extraction effect of acetone is better than distilled water and ethanol.Therefore determine to using acetone as the solvent that extracts bamboo sprout polyphenol.Next suitable acetone concentration is optimized.
Acetone concentration is set to 0%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%.
Collection Ji Zhu in May and the new fresh bamboo shoots of tea stalk bamboo, after liquid nitrogen freezing, break into pieces, Radix Crotalariae szemoensis meat is mixed in to-70 ℃ of preservations, during extraction, take out a certain amount of bamboo sprout and be placed in mortar, add liquid nitrogen to grind, the extraction solvent of various concentration is added to solvent in the ratio of bamboo sprout: solvent=1:10 (g:mL), homogenate, 10 ℃ of following low-temperature ultrasonics extract 15min, and 12000rpm centrifuging and taking supernatant, obtains bamboo sprout polyphenol extracting solution.
With reference to the detection system of having set up, detect the effect of various concentration solvent extraction polyphenol.The results are shown in Table 6.
The 755nm light absorption value of the various concentration solvent extraction of table 6 polyphenol
Experimental result shows that 30% acetone extraction effect is better.Gained bamboo sprout polyphenol extracting solution rotary distillation is removed to acetone, and then lyophilization just can obtain dried frozen aquatic products.
The optimization of acetone usage ratio
Embodiment 4:
Collection Ji Zhu in May and the new fresh bamboo shoots of tea stalk bamboo, after liquid nitrogen freezing, break into pieces, Radix Crotalariae szemoensis meat is mixed in to-70 ℃ of preservations, during extraction, take out a certain amount of bamboo sprout and be placed in mortar, add liquid nitrogen to grind, using 30% acetone as extracting solvent, bamboo sprout: the ratio of solvent (g:mL) is set to 1:5,1:10,1:15,1:20,1:25,1:30.In the broken end of bamboo sprout, add solvent, homogenate, 10 ℃ of following low-temperature ultrasonics extract 15min, 12000rpm centrifuging and taking supernatant.With reference to the detection system of having set up, detect the effect of various concentration solvent extraction polyphenol.Owing to adding the extraction solvent of different volumes in identical weight Folium Bambusae, to cause the few person's polyphenol concentration of volume higher, for polyphenol content in correcting unit solvent, in order to proofread and correct the solvent volume in each sample, in detection system, the consumption of polyphenol extracting solution is followed successively by 0.25mL, 0.5mL, 0.75mL, 1.0mL, 1.25mL, 1.5mL, water polishing is 1.5mL respectively, then adds respectively other composition in detection system.Testing result is in Table 7.
The 755nm light absorption value of the various ratio solvent extraction of table 7 polyphenol
When table 7 shows 30% acetone usage ratio by bamboo sprout: solvent=1:10 (g:mL), extract bamboo sprout polyphenol effect better.
The optimization of polyphenol extraction time
Embodiment 5:
In order to protect the quality of polyphenol, we select ultrasonoscope to extract polyphenol, the time of extracting are optimized simultaneously.Collection Ji Zhu in May and the new fresh bamboo shoots of tea stalk bamboo, after liquid nitrogen freezing, break into pieces, Radix Crotalariae szemoensis meat is mixed in to-70 ℃ of preservations, during extraction, take out a certain amount of bamboo sprout and be placed in mortar, add liquid nitrogen to grind, using 30% acetone as extracting solvent, in the ratio of Folium Bambusae: solvent=1:10 (g:mL), in the broken end of bamboo sprout, add solvent, homogenate, 10 ℃ of following low-temperature ultrasonics extract, and extraction time is set to 15min, 30min, 45min and 60min.After ultrasonic extraction finishes, 12000rpm centrifuging and taking supernatant.
With reference to the detection system of having set up, detect the effect of 30% acetone supersound extraction polyphenol within the various time.The results are shown in Table 8.
The various ultrasonic times of table 8 extract the 755nm light absorption value of polyphenol
| Time | 15min | 30min | 45min | 60min |
| λ755 | 0.7685 | 0.7712 | 0.8015 | 0.7809 |
Table 8 shows that ultrasonic 45min extraction effect is better.
Conclusion: in new fresh bamboo shoots, the optimum extraction process (system) of polyphenol is: 30% acetone is in 1:10(g:mL) ratio joins in bamboo sprout, homogenate, low temperature supersound extraction 45min, 12000rpm centrifuging and taking supernatant, the rotary distillation that reduces pressure is afterwards removed acetone, obtains bamboo sprout polyphenol extracting solution.
Three, polyphenol extraction process effect in bamboo sprout
Apply above-mentioned extraction system and the detection system of having set up, take gallic acid as standard specimen, detected the polyphenol content of 18 bamboo sprout kinds.Main process is: by frozen bamboo sprout pulverization under liquid nitrogen condition, take the Radix Crotalariae szemoensis end of 2g left and right, each kind arranges 3 repetitions, by 1:10(g:mL) solid-liquid ratio add 30% acetone of respective volume, homogenate, 10 ℃ of following low temperature supersound extraction 45min, 12000rpm centrifuging and taking supernatant, obtains the polyphenol extracting solution of bamboo sprout.According to following system, detect the polyphenol content in each extracting solution sample afterwards:
Detection system: polyphenol extracting solution 0.25mL(blank replaces extracting solution with 30% acetone), water 1.75mL, 6XFolin-ciocalteu developer 1mL, 15%Na
2cO
31mL.
Testing conditions: reaction temperature is 25 ℃ of water-baths, 1.5 hours response time, detection wavelength 755nm.
In relevant kind bamboo sprout, polyphenol content is in Table 9.
The content (mg/g) of polyphenol in the various new fresh bamboo shoots of table 9
| Bamboo sprout kind | Phyllostachys pubescens Mazei ex H.de Lebaie | Meyer bamboo | Black bamboo | Henon bamboo | Bamboo early | Tea stalk bamboo |
| Polyphenol content | 3.72 | 2.98 | 2.90 | 2.22 | 1.63 | 0.52 |
| Bamboo sprout kind | Red shell lei bamboo | Tip is blue or green | Phyllostachys nigra (lodd.ex lindl.) munro var.henonis (miff.) spapf et rendle | Phyllostachys congesta | Mottled bamboo | Anji gold bamboo |
| Polyphenol content | 1.61 | 1.604 | 1.51 | 1.41 | 1.38 | 1.37 |
| Bamboo sprout kind | Red bamboo | May Ji Zhu | White food in one's mouth chicken bamboo | Gold inlaid jade | Yellow stalk crow feeds chicken bamboo | Ph. bambusoides |
| Polyphenol content | 1.31 | 1.30 | 1.19 | 1.18 | 1.18 | 0.96 |
Note: in new fresh bamboo shoots, dry matter content is about 10%.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any modifications of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in protection scope of the present invention.
Claims (5)
1. the extraction of polyphenol and a detection method in bamboo sprout, is characterized in that: steps of the method are:
(1) get the raw materials ready: choose new fresh bamboo shoots, after liquid nitrogen freezing, break into pieces and shell, Radix Crotalariae szemoensis meat is in-70 ℃ of preservations, standby;
(2) polyphenol in the auxiliary organic solvent extract at low temperature bamboo sprout of ultrasound wave: during extraction, take out a certain amount of bamboo sprout and be placed in mortar, add liquid nitrogen to grind, choose acetone and make to extract solvent, in bamboo sprout, add acetone, after the abundant homogenate of sample, collect in centrifuge tube, in the 15～60min of low temperature supersound extraction below 10 ℃, centrifuging and taking supernatant, obtains bamboo sprout polyphenol extracting solution;
(3) detection of polyphenol in bamboo sprout:
Folin-ciocalteu method detects: in new fresh bamboo shoots, polyphenol detection system is: polyphenol extracting solution 0.25mL, water 1.75mL, dilute Folin-ciocalteu developer 1mL, the 5%～15%Na of 6 times
2cO
31mL;
Testing conditions: reaction temperature: 20～35 ℃ of water-baths, response time: 0.5～3.5h, detects wavelength: 755nm～770nm.
2. extraction and the detection method of polyphenol in a kind of bamboo sprout according to claim 1, it is characterized in that: in step (2), choose 30% acetone and make to extract solvent, by 1g bamboo sprout, add 5～30mL30% acetone, after the abundant homogenate of sample, collect in 50mL centrifuge tube, in the 15～60min of low temperature supersound extraction below 10 ℃, centrifuging and taking supernatant, obtains bamboo sprout polyphenol extracting solution.
3. extraction and the detection method of polyphenol in a kind of bamboo sprout according to claim 2, is characterized in that: by 1g bamboo sprout, preferably add 10mL30% acetone to extract.
4. extraction and the detection method of polyphenol in a kind of bamboo sprout according to claim 2, is characterized in that: supersound extraction frequency is 40kHz, ultrasonic power 150W, and extraction time is 45min.
5. extraction and the detection method of polyphenol in a kind of bamboo sprout according to claim 1, it is characterized in that: in step (3), Folin-ciocalteu method detects: in new fresh bamboo shoots, polyphenol detection system is: polyphenol extracting solution 0.25mL, water 1.75mL, dilute Folin-ciocalteu developer 1mL, the 15%Na of 6 times
2cO
31mL;
Testing conditions: reaction temperature: 25 ℃ of water-baths, response time: 1.5h, detects wavelength: 755nm.
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| CN104188007A (en) * | 2014-06-24 | 2014-12-10 | 正安县顶箐方竹笋有限公司 | Bamboo-shoot beverage and preparation method thereof |
| CN105021608A (en) * | 2015-08-20 | 2015-11-04 | 昆明理工大学 | Kit for detecting total polyphenol content in beverage, preparation method of kit and method for detecting total polyphenol content in beverage using kit |
| CN106124428A (en) * | 2016-06-14 | 2016-11-16 | 安徽平唐微食疗科技有限公司 | A kind of Determination of Polyphenols detection method |
| CN107121507A (en) * | 2017-04-20 | 2017-09-01 | 浙江大学 | A kind of bamboo shoot shell polyphenol substance assay method |
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| AT520055A1 (en) * | 2017-06-12 | 2018-12-15 | Omnignostica Forschungs Gmbh & Co Kg | Method for the colorimetric determination of polyphenols in biological fluids |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104188007A (en) * | 2014-06-24 | 2014-12-10 | 正安县顶箐方竹笋有限公司 | Bamboo-shoot beverage and preparation method thereof |
| CN104188007B (en) * | 2014-06-24 | 2015-06-24 | 正安县顶箐方竹笋有限公司 | Bamboo-shoot beverage and preparation method thereof |
| CN105021608A (en) * | 2015-08-20 | 2015-11-04 | 昆明理工大学 | Kit for detecting total polyphenol content in beverage, preparation method of kit and method for detecting total polyphenol content in beverage using kit |
| CN106124428A (en) * | 2016-06-14 | 2016-11-16 | 安徽平唐微食疗科技有限公司 | A kind of Determination of Polyphenols detection method |
| CN107121507A (en) * | 2017-04-20 | 2017-09-01 | 浙江大学 | A kind of bamboo shoot shell polyphenol substance assay method |
| CN107121507B (en) * | 2017-04-20 | 2019-08-20 | 浙江大学 | A kind of bamboo shoot shell polyphenol substance measuring method |
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