CN102908370A - Method for extracting polyphenolic compounds from seabuckthorn fruit - Google Patents
Method for extracting polyphenolic compounds from seabuckthorn fruit Download PDFInfo
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- CN102908370A CN102908370A CN2011102187979A CN201110218797A CN102908370A CN 102908370 A CN102908370 A CN 102908370A CN 2011102187979 A CN2011102187979 A CN 2011102187979A CN 201110218797 A CN201110218797 A CN 201110218797A CN 102908370 A CN102908370 A CN 102908370A
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Abstract
The invention provides a method for extracting polyphenolic compounds from seabuckthorn fruit. According to the invention, seabuckthorn dried fruit powder or dry seabuckthorn fruit pomace are well mixed with diatomite, and the mixture is subjected to extraction in an extraction tank; filtering is carried out after extraction is finished, such that an extraction liquid is obtained; the liquid is concentrated and lyophilized, such that polyphenolic compounds in seabuckthorn fruit are obtained. The method provided by the invention has the advantages of short extraction time, low solvent dosage, and high extraction efficiency.
Description
Technical field
The invention belongs to the extracted form natural plant technical field, be specifically related to from Fructus Hippophae, extract the method for polyphenol compound.
Background technology
Fructus Hippophae has another name called vinegar willow, Ci Liu, is shrub or the dungarunga of Elaeangnaceae acid thorn genus.Fructus Hippophae is the general designation of plant and its fruit.Fructus Hippophae is medicinal and edible plant.The root of Fructus Hippophae, stem, leaf, flower, really, particularly sea buckthorn fruit contains abundant nutrient substance and bioactive substance, can be widely used in many fields of the national economy such as food, medicine, light industry, space flight, agriculture and animal husbandry fishery.Sea buckthorn fruit be used as medicine have relieving cough and resolving phlegm, the effect of invigorating the stomach and promoting digestion, promoting blood circulation to remove blood stasis.Modern medicine study, sea buckthorn fruit have cough-relieving, relieving asthma and eliminate the phlegm, expand the usefulness of coronary vasodilator, cholesterol reducing, are usually used in treating ischemic heart desease, and treatment angina pectoris effective percentage is reached 94%.
Contain abundant polyphenol compound in the Fructus Hippophae, the major function character of polyphenol compound is as the inhibitor of many enzyme systems or activator, metal-chelator and free radical scavenger, is the very promising class Natural antioxidant and a free radical scavenger in medicine, food, the cosmetic industry.Have important researching value and application prospect widely so from Fructus Hippophae, extract polyphenol compound.
The method of extracting polyphenol compound from Fructus Hippophae that adopts at present has the methods such as microwave extraction, supersound extraction, and (Liu Lingling etc. use chemical industry, 2010,39 (5), 684-686; Fan Jinling etc., researches on natural drugs and exploitation, 2006,18:529-534,554), these methods exist extraction time long, and extracted amount is little, the shortcoming that cost is high.
Fast solvent extraction method (being called for short ASE) is the method that with solvent solid or semi-solid sample is extracted under certain temperature (50 ℃-200 ℃) and pressure (1000-3000psi or 10.3-20.6MPa).Use conventional solvent, utilization increase temperature and improve the efficient that pressure improves extraction, the time that its result has accelerated to extract is greatly also obviously reduced the use amount of extractant.
Quick solvent-extracted outstanding advantages: extract with Soxhlet, ultrasonic, microwave, supercritical compare with the classical traditional methods such as separatory funnel jolting, solvent extraction has following outstanding advantages fast: consumption of organic solvent is few, the 10g sample only needs the 15mL solvent, has reduced the processing of waste liquid; The time of fast, finishing the single extraction overall process generally only needs 15 minutes; Matrix effect is little, can carry out the semisolid extraction of solid (sample is moisture below 75%), can be with identical extraction conditions to different matrix; Because extraction process is vertical static extracting, can when the filling sample, add in advance filter course or adsorbing medium in the bottom; The method development is convenient, and the solvent-extracted method of mature usefulness is available quick solvent extraction all; Automaticity is high, can repeatedly extract the same sample as required, or change solvent extraction, and all these can be by user oneself programming, Automatic Control; Extraction efficiency is high, and selectivity is good; Property easy to use, safe is good.The application of ASE relates to environment, food, pharmacy and polymer arts.
Summary of the invention
The invention provides the method for extracting polyphenol compound from Fructus Hippophae, adopt quick solvent extraction method, the method solvent use amount is few, and extraction efficiency is high.
To achieve these goals, the invention provides the method for from Fructus Hippophae, extracting polyphenol compound, it is characterized in that: get Fructus Hippophae dry fruit powder or dry sea-buckthorn pomace, after processing with the normal hexane removing oil, add the kieselguhr mixing, be loaded on abstraction pool, take the ethanol water of 55-85% as extractant, extraction 10-15min is after extraction finishes, filtration is extracted liquid, and concentrated and lyophilization obtains the polyphenol compound in the sea buckthorn fruit.
Described kieselguhr consumption be Fructus Hippophae dry fruit powder or dry sea-buckthorn pomace quality 1.5-2.5 doubly.
Described extracting pressure is 12-20Mpa, and temperature is 55-75 ℃.
Useful technique effect of the present invention is: the present invention adopts quick solvent extraction, and extraction time is short, uses solvent few, and extract obtained middle polyphenol compound content is higher than traditional method.
The specific embodiment
Embodiment 1
Get Fructus Hippophae dry fruit powder or dry sea-buckthorn pomace 100g, after processing with the normal hexane removing oil, add 150g kieselguhr mixing, be loaded on abstraction pool, ethanol water take 70% is extractant, and extracting pressure is 15Mpa, and temperature is 60 ℃, extract 1 time, each 15min after extraction finishes, filters and is extracted liquid, concentrated and lyophilization obtains the polyphenol compound in the sea buckthorn fruit, and Determination of Polyphenols is 28.3%.(the sample total phenol content is measured and is adopted the diazo reagent colorimetry)
Embodiment 2
Get Fructus Hippophae dry fruit powder or dry sea-buckthorn pomace 400g, after processing with the normal hexane removing oil, add 1kg kieselguhr mixing, be loaded on abstraction pool, ethanol water take 55% is extractant, and extracting pressure is 12Mpa, and temperature is 75 ℃, extract 1 time, each 10min after extraction finishes, filters and is extracted liquid, concentrated and lyophilization obtains the polyphenol compound in the sea buckthorn fruit, and Determination of Polyphenols is 27.0%.(the sample total phenol content is measured and is adopted the diazo reagent colorimetry)
Embodiment 3
Get Fructus Hippophae dry fruit powder or dry sea-buckthorn pomace 400g, after processing with the normal hexane removing oil, add 800g kieselguhr mixing, be loaded on abstraction pool, ethanol water take 85% is extractant, and extracting pressure is 20Mpa, and temperature is 55 ℃, extract 1 time, each 15min after extraction finishes, filters and is extracted liquid, concentrated and lyophilization obtains the polyphenol compound in the sea buckthorn fruit, and Determination of Polyphenols is 23.8%.(the sample total phenol content is measured and is adopted the diazo reagent colorimetry)
Embodiment 4
Get Fructus Hippophae dry fruit powder or dry sea-buckthorn pomace 500g, after processing with the normal hexane removing oil, add 1kg kieselguhr mixing, be loaded on abstraction pool, ethanol water take 70% is extractant, and extracting pressure is 15Mpa, and temperature is 65 ℃, extract 2 times, each 15min after extraction finishes, filters and is extracted liquid, concentrated and lyophilization obtains the polyphenol compound in the sea buckthorn fruit, and Determination of Polyphenols is 28.1%.(the sample total phenol content is measured and is adopted the diazo reagent colorimetry)
Embodiment 5
Get Fructus Hippophae dry fruit powder or dry sea-buckthorn pomace 1kg, after processing with the normal hexane removing oil, add 1.5kg kieselguhr mixing, be loaded on abstraction pool, ethanol water take 55% is extractant, and extracting pressure is 18Mpa, and temperature is 65 ℃, extract 2 times, each 10min after extraction finishes, filters and is extracted liquid, concentrated and lyophilization obtains the polyphenol compound in the sea buckthorn fruit, and Determination of Polyphenols is 25.4%.(the sample total phenol content is measured and is adopted the diazo reagent colorimetry).
Claims (3)
1. from Fructus Hippophae, extract the method for polyphenol compound, it is characterized in that: get Fructus Hippophae dry fruit powder or dry sea-buckthorn pomace, after normal hexane removing oil processing, add the kieselguhr mixing, be loaded on abstraction pool, take the ethanol water of 55-85% as extractant, extraction 10-15min, after extraction finishes, filter and be extracted liquid, concentrated and lyophilization obtains the polyphenol compound in the sea buckthorn fruit.
2. the method for extracting polyphenol compound from Fructus Hippophae according to claim 1 is characterized in that: described kieselguhr consumption be Fructus Hippophae dry fruit powder or dry sea-buckthorn pomace quality 1.5-2.5 doubly.
3. the method for from Fructus Hippophae, extracting polyphenol compound according to claim 1, it is characterized in that: described extracting pressure is 12-20Mpa, temperature is 55-75 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102187979A CN102908370A (en) | 2011-08-02 | 2011-08-02 | Method for extracting polyphenolic compounds from seabuckthorn fruit |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102187979A CN102908370A (en) | 2011-08-02 | 2011-08-02 | Method for extracting polyphenolic compounds from seabuckthorn fruit |
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| CN102908370A true CN102908370A (en) | 2013-02-06 |
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| CN2011102187979A Pending CN102908370A (en) | 2011-08-02 | 2011-08-02 | Method for extracting polyphenolic compounds from seabuckthorn fruit |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105832801A (en) * | 2016-05-22 | 2016-08-10 | 深圳市先康达生物科技有限公司 | Extraction and separation method for macaenes and maca amides |
| CN107884400A (en) * | 2017-11-09 | 2018-04-06 | 无限极（中国）有限公司 | The quantitative detecting method of total polyphenols in a kind of Purple Perilla Seed Oil |
-
2011
- 2011-08-02 CN CN2011102187979A patent/CN102908370A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| 陈舰: "快速溶剂萃取-高效液相法测定茶叶中的多酚", 《东莞理工学院学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105832801A (en) * | 2016-05-22 | 2016-08-10 | 深圳市先康达生物科技有限公司 | Extraction and separation method for macaenes and maca amides |
| CN105832801B (en) * | 2016-05-22 | 2020-02-04 | 尹梦珍 | Extraction and separation method of macaene and macamide |
| CN107884400A (en) * | 2017-11-09 | 2018-04-06 | 无限极（中国）有限公司 | The quantitative detecting method of total polyphenols in a kind of Purple Perilla Seed Oil |
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Application publication date: 20130206 |