CN104382951A - Extracting method for algal polyphenols - Google Patents

Extracting method for algal polyphenols Download PDF

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Publication number
CN104382951A
CN104382951A CN201410732016.1A CN201410732016A CN104382951A CN 104382951 A CN104382951 A CN 104382951A CN 201410732016 A CN201410732016 A CN 201410732016A CN 104382951 A CN104382951 A CN 104382951A
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Prior art keywords
extracting method
euglena
kinds
polyphenol
microwave
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CN201410732016.1A
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Inventor
陈喜军
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Heilongjiang Zhongsheng Biological Engineering Co Ltd
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Heilongjiang Zhongsheng Biological Engineering Co Ltd
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Priority to CN201410732016.1A priority Critical patent/CN104382951A/en
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/02—Algae
    • A61K36/05—Chlorophycota or chlorophyta (green algae), e.g. Chlorella
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/02—Algae
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Chemical & Material Sciences (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Botany (AREA)
  • Medical Informatics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention discloses an extracting method for algal polyphenols. Three kinds of algal polyphenols are jointly extracted by taking three kinds of algae including chlorella pyrenoidosa, euglena and dunaliella salina as raw materials and ethanol as a solvent by virtue of microwaves. The extracting method for algal polyphenols, disclosed by the invention, has the advantages that (1) the process is simple and practical, and the process flow is safe; (2) no large equipment or instruments are needed, so that the production cost is low; and (3) the algal polyphenols in the three kinds of raw materials including the chlorella pyrenoidosa, the euglena and the dunaliella salina are simultaneously extracted, and the extracting method is suitable for treating a great number of samples.

Description

A kind of extracting method of seeweed polyphenol
Technical field
the present invention relates to a kind of extracting method, particularly a kind of method simultaneously extracting seeweed polyphenol from Chlorella pyrenoidesa, Euglena, salt alga.
Background technology
seeweed polyphenol (algae polyphenols, AP) is the polyphenol compound general name extracted from Sargassum, the unique physiological function had because of it and become the study hotspot in phenols field.Polyphenol is a large class formation difference, containing polyhydric compound general name, is extensively present in terrestrial plant.But along with modern analysis and isolation technics are applied to the exploitation of marine algae resource progressively deeply, seeweed polyphenol causes the concern of people day by day.From existing research, seeweed polyphenol content in the Sargassums such as Alga Sgrgassi Enerves, Fucus Vesiculosus, Ectocarpus is abundanter, but along with people use analysis of modernization, isolation technics is studied Sargassum deepens continuously, in increasing algae, extract polyphenol substance.
seeweed polyphenol is the different compound of a large class formation, and research finds that brown algae polyphenols has anti-tumor activity, antibacterial, antiviral activity, antioxidant activity, chemical defence activity, deodorant activities etc.Seeweed polyphenol is a kind of safety non-toxic, has the native chemical product of unique bioactive.China's marine algae resource enriches, and should utilize this resources advantage, and in conjunction with the biological characteristics of seeweed polyphenol uniqueness, research and development novel sea medicine and Biofunctional goods.
Summary of the invention
the object of the present invention is to provide a kind of simple, fast and efficient while in Chlorella pyrenoidesa, Euglena, salt alga, extract the method for polyphenol substance.
object of the present invention is achieved through the following technical solutions: a kind of extracting method of seeweed polyphenol, with Chlorella pyrenoidesa, Euglena, salt alga three kinds of algae for raw material, is solvent, adopts the polyphenol of microwave combined extraction three kinds of algae with ethanol.
the extracting method of described a kind of seeweed polyphenol, the key step of its extracting method is:
(1) gather Chlorella pyrenoidesa, Euglena, salt alga three kinds of raw materials respectively, cleaning, drying, then pulverized 40 mesh sieves;
(2) take the Chlorella pyrenoidesa after pulverizing and sieving, Euglena, salt alga, 1:1:1 mixing in mass ratio, then add the alcoholic solution that concentration is 10-80%, solid-liquid ratio is 20-100ml/g, and agitation as appropriate is even, obtains material liquid mixture;
(3) material liquid mixture of step 2 being placed in microwave power is that the microwave field of 500-1500W extracts, and microwave treatment time is 20-100s, obtains mixed extract;
(4) filtered by mixed extract, filtrate lyophilization, obtains seeweed polyphenol extract.
beneficial effect of the present invention:
(1) features simple and practical process, technological process safety.
(2) do not need main equipment instrument, production cost is low.
(3) realize simultaneously to the extraction of Chlorella pyrenoidesa, Euglena, salt alga three kinds of raw material seeweed polyphenols, and be applicable to the process of a large amount of sample.
embodiment 1
seeweed polyphenol is extracted with following steps:
(1) gather Chlorella pyrenoidesa, Euglena, salt alga three kinds of raw materials respectively, cleaning, drying, as conglomeration can pulverize 40 mesh sieves;
(2) take the 1:1:1 mixing in mass ratio of Chlorella pyrenoidesa, Euglena, salt alga, then add the alcoholic solution that concentration is 30%, solid-liquid ratio is 10ml/g, and agitation as appropriate is even.
(3) mixture of step 2 being placed in microwave power is that the microwave field of 800W extracts, and microwave treatment time is 20s.
(3) filter, filtrate lyophilization obtains polyphenol extract.
seeweed polyphenol extraction ratio is 2.51%.
embodiment 2
seeweed polyphenol is extracted with following steps:
(1) gather Chlorella pyrenoidesa, Euglena, salt alga three kinds of raw materials respectively, cleaning, drying, as conglomeration can pulverize 40 mesh sieves;
(2) take the 1:1:1 mixing in mass ratio of Chlorella pyrenoidesa, Euglena, salt alga, then add the alcoholic solution that concentration is 20%, solid-liquid ratio is 20ml/g, and agitation as appropriate is even.
(3) mixture of step 2 being placed in microwave power is that the microwave field of 800W extracts, and microwave treatment time is 40s.
(3) filter, filtrate lyophilization obtains polyphenol extract.
seeweed polyphenol extraction ratio is 3.08%.
embodiment 3
seeweed polyphenol is extracted with following steps:
(1) gather Chlorella pyrenoidesa, Euglena, salt alga three kinds of raw materials respectively, cleaning, drying, as conglomeration can pulverize 40 mesh sieves;
(2) take the 1:1:1 mixing in mass ratio of Chlorella pyrenoidesa, Euglena, salt alga, then add the alcoholic solution that concentration is 10%, solid-liquid ratio is 30ml/g, and agitation as appropriate is even.
(3) mixture of step 2 being placed in microwave power is that the microwave field of 800W extracts, and microwave treatment time is 60s.
(3) filter, filtrate lyophilization obtains polyphenol extract.
seeweed polyphenol extraction ratio is 2.11%.

Claims (2)

1. an extracting method for seeweed polyphenol, is characterized in that: with Chlorella pyrenoidesa, Euglena, salt alga three kinds of algae for raw material, be solvent, adopt the polyphenol of microwave combined extraction three kinds of algae with ethanol.
2. the extracting method of a kind of seeweed polyphenol according to claim 1, is characterized in that: the key step of its extracting method is:
(1) gather Chlorella pyrenoidesa, Euglena, salt alga three kinds of raw materials respectively, cleaning, drying, then pulverized 40 mesh sieves;
(2) take the Chlorella pyrenoidesa after pulverizing and sieving, Euglena, salt alga, 1:1:1 mixing in mass ratio, then add the alcoholic solution that concentration is 10-80%, solid-liquid ratio is 20-100ml/g, and agitation as appropriate is even, obtains material liquid mixture;
(3) material liquid mixture of step 2 being placed in microwave power is that the microwave field of 500-1500W extracts, and microwave treatment time is 20-100s, obtains mixed extract;
(4) filtered by mixed extract, filtrate lyophilization, obtains seeweed polyphenol extract.
CN201410732016.1A 2014-12-06 2014-12-06 Extracting method for algal polyphenols Pending CN104382951A (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105193863A (en) * 2015-10-09 2015-12-30 集美大学 Preparation method of high-purity algal polyphenol
CN107397189A (en) * 2017-07-20 2017-11-28 浙江省海洋开发研究院 A kind of fresh shrimp taste instant noodles seasoning powder and preparation method thereof
CN107960629A (en) * 2017-07-20 2018-04-27 浙江省海洋开发研究院 A kind of seafood shrimp taste soup jelly and preparation method thereof
CN108434177A (en) * 2018-03-30 2018-08-24 中国农业科学院烟草研究所 A kind of activity inhibitor and preparation method thereof and its in application hypoglycemic, in reducing blood lipid
CN108669555A (en) * 2018-04-17 2018-10-19 北京中和鸿业医药科技有限公司 A kind of Algal Assemblages object, preparation, preparation method and applications
CN108697741A (en) * 2016-03-04 2018-10-23 悠绿那股份有限公司 Antivirotic and antiviral use food
CN116458542A (en) * 2023-03-23 2023-07-21 大连工业大学 Alga polyphenol extract capable of inhibiting autolysis of sea cucumbers, and preparation method and application thereof

Citations (3)

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Publication number Priority date Publication date Assignee Title
CN102166230A (en) * 2011-04-14 2011-08-31 大连民族学院 Microwave-assisted extraction method of seaweed polyphenol
CN102935093A (en) * 2012-12-10 2013-02-20 青岛海尔软件有限公司 Extraction method of seaweed polyphenols
WO2014050390A1 (en) * 2012-09-25 2014-04-03 富士フイルム株式会社 Composition for food and fat absorption inhibitor

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102166230A (en) * 2011-04-14 2011-08-31 大连民族学院 Microwave-assisted extraction method of seaweed polyphenol
WO2014050390A1 (en) * 2012-09-25 2014-04-03 富士フイルム株式会社 Composition for food and fat absorption inhibitor
CN102935093A (en) * 2012-12-10 2013-02-20 青岛海尔软件有限公司 Extraction method of seaweed polyphenols

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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105193863A (en) * 2015-10-09 2015-12-30 集美大学 Preparation method of high-purity algal polyphenol
CN105193863B (en) * 2015-10-09 2018-10-16 集美大学 A kind of preparation method of high-purity seeweed polyphenol
CN108697741A (en) * 2016-03-04 2018-10-23 悠绿那股份有限公司 Antivirotic and antiviral use food
CN107397189A (en) * 2017-07-20 2017-11-28 浙江省海洋开发研究院 A kind of fresh shrimp taste instant noodles seasoning powder and preparation method thereof
CN107960629A (en) * 2017-07-20 2018-04-27 浙江省海洋开发研究院 A kind of seafood shrimp taste soup jelly and preparation method thereof
CN107960629B (en) * 2017-07-20 2021-01-15 浙江省海洋开发研究院 Seafood and shrimp-flavor soup jelly and preparation method thereof
CN108434177A (en) * 2018-03-30 2018-08-24 中国农业科学院烟草研究所 A kind of activity inhibitor and preparation method thereof and its in application hypoglycemic, in reducing blood lipid
CN108669555A (en) * 2018-04-17 2018-10-19 北京中和鸿业医药科技有限公司 A kind of Algal Assemblages object, preparation, preparation method and applications
CN116458542A (en) * 2023-03-23 2023-07-21 大连工业大学 Alga polyphenol extract capable of inhibiting autolysis of sea cucumbers, and preparation method and application thereof

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