CN103585409A - Polygonum cuspidatum and glossy privet fruit mixture and preparation method thereof - Google Patents
Polygonum cuspidatum and glossy privet fruit mixture and preparation method thereof Download PDFInfo
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- CN103585409A CN103585409A CN201310575889.1A CN201310575889A CN103585409A CN 103585409 A CN103585409 A CN 103585409A CN 201310575889 A CN201310575889 A CN 201310575889A CN 103585409 A CN103585409 A CN 103585409A
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Abstract
The invention relates to an animal medicine, namely a polygonum cuspidatum and glossy privet fruit mixture. The mixture is characterized in that 100ml mixture is prepared by 15-25g polygonum cuspidatum, 15-18g glossy privet fruit, 2-5g perfoliate knotweed, 2-5g Chinese yam, 1-2g astragalus mongholicus, 2-6g aizoon stonecrop, 3-6g hypericum japonicum, 1-4g sculellaria barbata, 2-5g myrobalan and 2-5g dandelion. A preparation method of the mixture comprises the steps that the ten traditional Chinese medicines in a formula are decocted for three times by water, decocted for 2h for the first time, and decocted for 1h for the second time and the third time respectively; decoctions are merged and filtered; a filtrate is concentrated to be about 90ml, and cooled; acetic acid is added; pH (power of hydrogen) is adjusted to be 3.0-3.5; filtering is performed; a filtrate is diluted to be 100ml by the water; encapsulation is performed; and the mixture is obtained. The prepared polygonum cuspidatum and glossy privet fruit mixture is simple in technology, high in bioavailability, good in effect, low in cost and convenient to use.
Description
Technical field
The present invention relates to medicine field for animals, be specially a kind of for preventing and treating pig breeding and breathing syndrome, circovurus type 2 infection " brave loyal mixture " and preparation method thereof.
Background technology
Fast development along with animal husbandry, being accompanied by poultry breeding introduces, add the impact of the various stress factors such as environmental factors, swine diseases is now complicated, and the diseases such as pig breeding and breathing syndrome (PRRS), circovirus disease (PCV) have become the key factor that affected in recent years animal husbandry economy benefit and restriction animal husbandry sound development.
Breeding and breathing syndrome (PRRS), claim again " reproductive and respiratory syndrome ", a kind of breeding and the respiratory infectious disease ，Bei International Office of Epizootics (OIE) that are the pig that causes of breeding and breathing syndrome virus (PRRSV) by Arteriviridae, Arterivirus list category-B infectious disease in by (PRRSV).Primary disease is mainly through respiratory tract, digestive tract infection, simultaneously also can vertical transmission, and the pig of various ages, sex, kind all can be felt.This disease is mainly encroached on brood sow and piglet, main manifestations anorexia, heating, miscarriage later stage of pregnancy, stillborn fetus and mummy tire; 2～28 age in days Infection in Piglets symptoms are obvious, and mortality rate is up to 80%, especially farrowing of premature pig.Boar infects and causes semen quality to decline, the symptoms such as postponement of oestrusing.For this disease in the market without specific medicament therapy.
Circovirus disease (PCV), claim again pmws, by porcine circovirus, caused a kind of new important immunosuppressant sexually transmitted disease of pig, main infection pig in 8～13 week age, infects that pig performance body constitution declines, becomes thin, pale, jaundice, lymphadenectasis, diarrhoea, dyspnea.Circovirus porcine circovirus section, Circovirus, without cyst membrane, Single-stranded DNA virus, environment and disinfectant resistance are more intense to external world.This disease pathogen is divided into PCV1 and two kinds of serotypes of PCV2, and the generation of PCV2 and various diseases is closely related, comprises the scorching nephrotic syndrome PDNS of ablactational baby pig multisystem wasting syndrome PMWS and Corii Sus domestica etc., and PCV1 no pathogenicity.Primary disease has stronger infectivity, can direct oral cavity, respiratory tract approach infects the swinery of all ages and classes, and can vertical transmission.Simultaneously PCV normal with pig parvoviral (PPV) or pig breeding and respiratory disorder syndrome (PRRS) mixed infection, cause many accessory symptomses, make the diagnosis of disease more be tending towards complicated and diversified.For example, by PCV-2, infected and cause that the probability that PMWS occurs after ablactational baby pig and young pig is 4%～25%, but case fatality rate but reaches more than 90%.It is reported, PCV may be the main cause that causes PMWS, although be not sole cause.Therefore, this disease is brought very large economic loss to pig industry.
Summary of the invention
Technical problem solved by the invention is to provide a kind of " brave loyal mixture " and preparation method thereof, to solve the problem in background technology.
Technical problem to be solved by this invention realizes by the following technical solutions: " brave loyal mixture " by weight, raw material is composed as follows: Rhizoma Polygoni Cuspidati 15～25g, Fructus Ligustri Lucidi 15～18g, Herba Polygoni cymosi 2～5g, Rhizoma Dioscoreae 2～5g, the Radix Astragali 1～2g, Herba Sedi Aizoon 2～6g, Herba Hyperici Japonici 3～6g, Herba Scutellariae Barbatae 1～4g, Fructus Chebulae 2～5g and Herba Taraxaci 2～5g.
By following processing step and technological parameter, prepare: above ten tastes, decoct with water 2 hours for the first time three times, second and third time each 1 hour, collecting decoction, filters, and filtrate is concentrated into approximately 90 ml, let cool, with vinegar acid for adjusting pH, 3.0～3.5, filter, filtrate is diluted with water to 100ml, embedding, obtains.
In the present invention, raw material composition is preferably by weight: Rhizoma Polygoni Cuspidati 20g, and Fructus Ligustri Lucidi 16g, Herba Polygoni cymosi 4g, Rhizoma Dioscoreae 3g, Radix Astragali 1g, Herba Sedi Aizoon 3g, Herba Hyperici Japonici 4g, Herba Scutellariae Barbatae 3g, Fructus Chebulae 3g and Herba Taraxaci 3g, make " brave loyal mixture " 100ml.
The present invention is sepia thick liquid, and gas is fragrant.There is heat-clearing and toxic substances removing, benefiting QI for strengthening the superficies, removing dampness sore effect, for preventing and treating pig breeding and breathing syndrome, circovurus type 2 infection.
Authentication method of the present invention is as follows: (1) gets this product 2ml, adds water to 10ml, puts in round-bottomed flask, add 1ml hydrochloric acid, reflux 30 minutes, adds chloroform 20ml, continue reflux 30 minutes, take out, let cool, put in separatory funnel, divide and get chloroform layer, evaporate to dryness, residue dissolves with chloroform, and be settled to 1ml, as need testing solution.Get Rhizoma Polygoni Cuspidati control medicinal material 0.2g, add water 20ml, be made in the same way of control medicinal material solution.Another get respectively emodin, physcione adds chloroform and makes every 1ml containing the solution of 1mg, in contrast product solution.According to thin layer chromatography (two appendix of < < Chinese veterinary pharmacopoeia > > version in 2010), test, draw each 5 μ l of above-mentioned solution, put respectively on same silica gel g thin-layer plate, toluene-ethyl acetate-36% acetic acid (15:0.5:0.1) of take is developing solvent, launch, take out, dry, put under ultra-violet lamp (365nm) and inspect.In test sample chromatograph, with the corresponding position of reference substance chromatograph on, the fluorescence speckle of aobvious same color.
(2) get this product 10ml, with sodium hydroxide test solution, regulate pH value to 7.0, add chloroform 30ml, heating in water bath refluxes 30 minutes, move in separatory funnel, divide and get evaporate to dryness in the water-bath of chloroform stratification, residue adds dehydrated alcohol-chloroform (3:2) mixed liquor 1ml to be made to dissolve, as need testing solution.Separately get Fructus Ligustri Lucidi control medicinal material 1g, add dehydrated alcohol supersound process 20 minutes, filter, filtrate water bath method, residue adds dehydrated alcohol-chloroform (3:2) mixed liquor 1ml to be made to dissolve, in contrast medical material solution.According to thin layer chromatography (two appendix of < < Chinese veterinary pharmacopoeia > > version in 2010), test, draw each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, thiacyclohexane-the acetone and ethyl acetate (5:2:1) of take is developing solvent, launch, take out, dry, spray is with 1.8mol/L ethanol solution of sulfuric acid, and 110 ℃ to be dried to speckle colour developing clear.In test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, the speckle of aobvious same color.
(3) get this product 20ml, salt adding acid for adjusting pH value to 2.0, lets cool, and is extracted with ethyl acetate twice, each 20ml, and combined ethyl acetate liquid, evaporate to dryness, residue dissolves with ethyl acetate 1ml, as need testing solution.Get again gallic acid reference substance, add ethyl acetate and make every 1ml containing the solution of 1mg, in contrast product solution.According to thin layer chromatography (two appendix of < < Chinese veterinary pharmacopoeia > > version in 2010), test, draw each 5 μ l of above-mentioned three kinds of solution, put respectively on same silica gel g thin-layer plate, toluene-ethyl acetate-formic acid-water (5:7:2:2.9) upper strata liquid of take is developing solvent, launch, take out, dry, spray is with 1% ferric chloride test solution.In test sample chromatograph, with the corresponding position of reference substance chromatograph on, the speckle of aobvious same color.
Clinical test results of the present invention is as follows: (1) has selected 130 of the sick pigs of natural infection reproductive and respiratory syndrome, the high, medium and low dosage group, western medicine group and the blank group that are divided at random Chinese medicine, " brave loyal mixture " spray delivery for Chinese drug-treated group, No. 1, western medicine group intramuscular injection ampicillin and antiviral, equal every day 2 times, be used in conjunction 3 days; The not administration of blank group, 1 week statistics curative effect after processing.Therapeutic outcome shows, the cure rate of high, the middle dosage group of Chinese medicine and total effective rate be apparently higher than blank group and low dose group, with western medicine group without significant difference.Prompting " brave loyal mixture " has good curative effect to Porcine reproductive and respiratory syndrome.
(2) select natural infection circovurus type 2 and infected 56 of disease pigs, be divided at random high, medium and low dosage group, treatment matched group and the blank group of Chinese medicine." brave loyal mixture " spray delivery for Chinese drug-treated group, administration every day 2 times, is used in conjunction 3 days; No. 1, treatment matched group intramuscular injection ceftiofur and antiviral, administration every day 2 times, is used in conjunction 3 days; The not administration of blank group; Each group is 1 week statistics curative effect after processing.Therapeutic outcome shows, cure rate and the total effective rate of high, the middle dosage group of Chinese medicine are significantly higher than blank group and low dose group, with treatment matched group without significant difference.Prompting " brave loyal mixture " has good curative effect to porcine circovirus type 2 infection.
By " test of the test of brave loyal Mixture in treatment Porcine reproductive and respiratory syndrome and the porcine circovirus type 2 infection for the treatment of proves that the present invention can effectively prevent and treat the diseases such as porcine circovirus infection of Porcine reproductive and respiratory syndrome and pig.
the specific embodiment:
Be below embodiments of the invention, actual use of the present invention is not limited to embodiment.
Rhizoma Polygoni Cuspidati 100kg, Fructus Ligustri Lucidi 80kg, Herba Polygoni cymosi 20kg, Rhizoma Dioscoreae 15kg, Radix Astragali 5kg, Herba Sedi Aizoon 15kg, Herba Hyperici Japonici 20kg, Herba Scutellariae Barbatae 15kg, Fructus Chebulae 15kg and Herba Taraxaci 15kg.
Preparation process is as follows: above ten tastes, and add for the first time water 3000kg and decoct 2 hours, filter, filtering residue adds respectively water 1500kg, 1000kg and decocts twice again, each 1 hour, merges decocting liquid three times, filter, filtrate is concentrated into about 450L, lets cool, with vinegar acid for adjusting pH 3.0～3.5, filter, filtrate is diluted with water to 500L, and fill is to the brown medicinal glass bottle of 100ml, sealing, obtains.
Above specific embodiments of the invention be have been described in detail, but it is just as example, the present invention is not restricted to specific embodiment described above.To those skilled in the art, any equivalent modifications that the present invention is carried out and alternative also all among category of the present invention.Therefore,, not departing from the equalization conversion of doing under spirit of the present invention and category and revising, all should contain within the scope of the invention.
Claims (3)
1. medicine for animals " brave loyal mixture ", by weight, raw material is composed as follows: Rhizoma Polygoni Cuspidati 15～25g, Fructus Ligustri Lucidi 15～18g, Herba Polygoni cymosi 2～5g, Rhizoma Dioscoreae 2～5g, the Radix Astragali 1～2g, Herba Sedi Aizoon 2～6g, Herba Hyperici Japonici 3～6g, Herba Scutellariae Barbatae 1～4g, Fructus Chebulae 2～5g and Herba Taraxaci 2～5g.
2. " brave loyal mixture " as claimed in claim 1, its method for making is: Ten Herbs in general side, decocts with water three times, 2 hours for the first time, second and third time each 1 hour, collecting decoction, filter, filtrate is concentrated into about 90ml, let cool, with vinegar acid for adjusting pH 3.0～3.5, filter, filtrate is diluted with water to 100ml, and embedding obtains.
3. " the brave loyal mixture " as described in claim 1～2, its formula optimization is: every 100ml is containing Rhizoma Polygoni Cuspidati 20g, Fructus Ligustri Lucidi 16g, Herba Polygoni cymosi 4g, Rhizoma Dioscoreae 3g, Radix Astragali 1g, Herba Sedi Aizoon 3g, Herba Hyperici Japonici 4g, Herba Scutellariae Barbatae 3g, Fructus Chebulae 3g and Herba Taraxaci 3g.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105726654A (en) * | 2014-12-12 | 2016-07-06 | 北京大北农动物保健科技有限责任公司 | Anti-viral traditional Chinese medicine composition, premix, concentrate, complex material and application |
| CN107998332A (en) * | 2018-02-05 | 2018-05-08 | 河南翔大生物科技有限公司 | A kind of Chinese medicine composition for treating pig blue-ear disease and its application |
| CN108037234A (en) * | 2017-12-05 | 2018-05-15 | 广东益和堂制药有限公司 | A kind of quality determining method of Guanule contg. ' Jigucao particle |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1965978A (en) * | 2006-11-09 | 2007-05-23 | 扬中牧乐药业有限公司 | Natural botanical extraction liquid for preventing and treating epidemic febrile disease of birds and livestock and method for preparing same |
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2013
- 2013-11-18 CN CN201310575889.1A patent/CN103585409A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1965978A (en) * | 2006-11-09 | 2007-05-23 | 扬中牧乐药业有限公司 | Natural botanical extraction liquid for preventing and treating epidemic febrile disease of birds and livestock and method for preparing same |
Non-Patent Citations (1)
| Title |
|---|
| 利奕成等: "虎贞痛风胶囊对家兔急性关节炎的影响", 《中国病理生理杂》, vol. 25, no. 6, 31 December 2009 (2009-12-31), pages 1175 - 1180 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105726654A (en) * | 2014-12-12 | 2016-07-06 | 北京大北农动物保健科技有限责任公司 | Anti-viral traditional Chinese medicine composition, premix, concentrate, complex material and application |
| CN108037234A (en) * | 2017-12-05 | 2018-05-15 | 广东益和堂制药有限公司 | A kind of quality determining method of Guanule contg. ' Jigucao particle |
| CN107998332A (en) * | 2018-02-05 | 2018-05-08 | 河南翔大生物科技有限公司 | A kind of Chinese medicine composition for treating pig blue-ear disease and its application |
| CN107998332B (en) * | 2018-02-05 | 2021-02-19 | 河南翔大生物科技有限公司 | Traditional Chinese medicine composition for treating porcine reproductive and respiratory syndrome and application thereof |
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Application publication date: 20140219 |