CN101843682A - Maxingshigan injecta and preparation method thereof - Google Patents

Abstract

The invention discloses Maxingshigan injecta and a preparation method thereof, and the injecta comprises the following main components in parts by weight: 400-600 parts of gypsum, 400-600 parts of ephedra, 400-600 parts of bitter apricot seed and 400-500 parts of liquorice. The preparation method is implemented as follows: crushing the four medicinal materials, evenly mixing, adding 3000-7000 parts of water for decocting twice, decocting for 2h each time, mixing decocted solutions, filtering, decompressing and concentrating, cooling, adding ethanol to lead the ethanol content to reach 60%, evenly stirring, refrigerating, filtering, concentrating filtrate, recovering ethanol, refrigerating, filtering, adding activated carbon for decoloring, refrigerating, adding Tween 80 under stirring, adding injection water until the volume is 1000ml, encapsulating, and sterilizing to obtain a product.

Description

Maxingshigan injecta and preparation method thereof

Technical field

The present invention relates to the field, antibiotic medicine, be specially and be used for the treatment of chicken bronchitis, pneumonia, upper respiratory tract infection Maxingshigan injecta and preparation method thereof.

Background technology

Present medical treatment chicken bronchitis, pneumonia, upper respiratory tract infection, often effect is undesirable, generally adopts electrolyte, vitamin and glucose can reduce its death, in order to reduce the bacterial infection complication, also can use antibacterials, but these medicine dependencies are more intense, side effect is very big.

Maxingshigan injecta is according to " People's Republic of China's veterinary drug allusion quotation " two the 414th page " MAXINGSHIGANGSAN " sides of 2 years versions and 30 pages of " mahuang xingren gancao shigao decoction " sides of " pharmacology of Chinese medical formulae " (one), makes injection, and this preparation has heat clearing away simultaneously, lung qi dispersing, the effect of relievining asthma, the relieving cough and relieving asthma injection is to attack lung by wind heat, or the wind and cold heat-transformation, stop up and to hold back due to the lung, wind heat is stopped up lung, impairment of dispersing and descending function of the lung, so cough is breathed heavily, heat is stopped up puzzled, consumption impairment of body fluid liquid is so fever of the body is gradually even thirsty.Lung is stopped up QI rising in reverse order, and folding mistake department so antiperspirant or lossless is arranged, controls when the diffusing heresy of lung qi dispersing, clear pathogen, eliminating heat from the lung.The traditional Chinese medical science and herbal medicine be to above-mentioned disease, and it is more to treat effective prescription.

Summary of the invention

Technical problem solved by the invention is to provide a kind of Maxingshigan injecta and preparation method thereof, to solve the problem in the background technology:

Technical problem solved by the invention realizes by the following technical solutions:

1, the key component of Maxingshigan injecta is formed, and counts by weight, and raw material is composed as follows: Gypsum Fibrosum 400--600 part, Herba Ephedrae 400-600 part, Semen Armeniacae Amarum 400--600 part, Radix Glycyrrhizae 400-500 part;

The Gypsum Fibrosum external can reduce the vascular permeability face and play antiinflammation; Herba Ephedrae suffering, little hardship, temperature is returned lung, urinary bladder channel, and diaphoresis is relievingd asthma, diuresis, antiinflammatory, antiallergic, are eliminated the phlegm analgesic, antibiotic, antiviral at antitussive; Antitussive, antiasthmatic effect, antiinflammatory, analgesic activity, antitumor action, the Radix Glycyrrhizae function cures mainly heat-clearing and toxic substances removing, expelling phlegm for arresting cough, gastral cavity abdomen etc.

In the present invention, the optimum weight proportioning is as follows: 500 parts in Gypsum Fibrosum, 500 parts in Herba Ephedrae, 500 parts of Semen Armeniacae Amarums, 500 parts in Radix Glycyrrhizae.

2. and by following processing step and technological parameter prepare:

With Gypsum Fibrosum 400--600 part, Herba Ephedrae 400--600 part, Semen Armeniacae Amarum 400--600 part, Radix Glycyrrhizae 400-500 part; The broken mix homogeneously of 4 flavor medicated powder; Add water 3000--7000 part and decoct twice, for the first time, respectively decocted 2 hours for the second time, collecting decoction filters, concentrating under reduced pressure, and cooling adds ethanol and makes and contain the alcohol amount and reach 60%, stirs evenly, and cold preservation filters, and filtrate concentrates, and reclaims ethanol, cold preservation.Filter, filtrate adds activated carbon decolorizing, and cold preservation under agitation adds Tween 80, adds the injection water to 1000ml, embedding, and sterilization gets final product.

In the present invention, Zui Jia amount of water is 5000 parts.

The present invention is brown clear liquid; PH value is 3.5～5.5, cures mainly chicken bronchitis, pneumonia, upper respiratory tract infection, and reducing heat and dispersing lung-QI is arranged, the effect of relieving cough and relieving asthma, intramuscular injection, the every 1kg body weight of chicken 0.15ml, 2 times on the one.

Authentication method of the present invention has 2 kinds, and is as follows:

(1) gets this product 2ml, add 1～2 of water 5ml and dilute hydrochloric acid, boiled 2～3 minutes, filter.Filtrate is put in the separatory funnel, adds the ammonia solution number and droplet makes alkalize, adds chloroform 5ml again, and jolting is extracted.Divide and get chloroform solution, put in two test tubes, each 5 of a pipe ammonification system chlorinated ketone test solution and Carbon bisulfide, jolting is left standstill, and chloroform layer shows buff; Another pipe replaces Carbon bisulfide, the colourless or micro-yellow of chloroform layer after the jolting for blank with 5 of chloroforms.

(2) get this product 10ml, the 20ml that adds diethyl ether put in the water-bath reflux 30 minutes, discard ether solution, residue adds methanol 20ml, puts in the water-bath reflux 1 hour, filter, filtrate evaporate to dryness, residue add water 30ml makes dissolving, aqueous solution n-butanol extraction 3 times, each 15ml merges n-butyl alcohol liquid, with the saturated water washing of n-butyl alcohol 3 times, discard water liquid, n-butyl alcohol liquid is put evaporate to dryness in the water-bath, residue adds methanol 5ml makes dissolving, as need testing solution.Extracting liquorice acid ammonium reference substance adds methanol and makes the solution that every 1ml contains 2mg, in contrast product solution in addition.Test according to thin layer chromatography, draw each 5 μ l of above-mentioned two kinds of solution, put respectively in same be on the silica GF254 lamellae of adhesive with the sodium carboxymethyl cellulose, upper strata liquid with n-butyl alcohol-glacial acetic acid-water (6: 1: 3) is developing solvent, launch, take out, dry, put under the ultra-violet lamp (254nm) and inspect.In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the speckle of same color.

Clinical test results of the present invention is as follows:

(1) chooses the assorted chickling of health-care stone qi of 14 ages in days, totally 180, be divided into 6 groups at random, every group 30, grouping is raised, and free choice feeding drinking-water is by only weighing, be divided into 6 groups at random, and adjust that respectively to organize body weight basic identical, be respectively Maxingshigan injecta (large, medium and small) dosage group, powerful cough with asthma spirit group (routine administration group), infect not administration group (positive controls), do not infect not administration group (negative control group), except that negative control group, give chicken trachea virus inoculation KIB-C1 strain, the medication group medicine of table 1 prescribed dose that begins to feed after 16 hours, successive administration 7 days.Observe every day cough, stridulates, respiratory symptoms such as breathing, gong sound, spiritual, appetite and feces situation of change.Dead chicken is cutd open inspection, determine the cause of the death.And every group of chicken tested front and back weigh, weigh before weighing and stop the feed material one evening, observed for 2 weeks; Draft high, medium and low dosage, wherein low dose is preventive dose (intramuscular injection 0.1ml/mg), and middle dosage is therapeutic dose (intramuscular injection 0.2ml/mg), and heavy dose is (intramuscular injection 0.3ml/mg); Control drug is powerful cough with asthma spirit, by its description, and the administration of every chicken 1ml every day drinking-water.

Design experiment, route of administration and therapeutic dose are made table 1, table 2:

Table 1 test grouping and processing

Table 2 interpretation is compared with the infection matched group: ^{△ △}P＜0.01; Compare with powerful antasthmatic: ^*P＜0.05)

Off-test average body average weight gain is cured the enabledisable total effective rate

Group

Weight/g only ^-1/ g only ^-1(only) (only) (only) (%)

Normal healthy controls group 325.08 ± 25.20 190.71----

Infect matched group 223.37 ± 35.75 100.44--13

Powerful cough with asthma spirit 267.47 ± 33.45 141.44 20 24 6 80.0%

The relieving cough and relieving asthma injection

269.71±49.36 148.12 24 29 3 96.7％ ^△△*

Liquid (heavy dose)

The relieving cough and relieving asthma injection

263.15±24.74 142.75 26 29 29 6.7％ ^△△*

Liquid (middle dosage)

The relieving cough and relieving asthma injection

245.83±47.05 125.15 20 23 7 76.7％ ^△△

Liquid (low dose)

By experimental result as can be known, respiratory symptoms such as gong sound appearred coughing, stridulating, breathe in back 16 hours respectively in each counteracting toxic substances group in infection, but appetite is normal with drinking-water; Purging pathogenic fire of the lung color loose stool after negative control group 4-5 days, part chicken occur spirit depressed, stay minimizings of standing, search for food, foot is dried, chicken began death in the 6th day, stopped after dead 6 days continuously; Respiratory symptoms such as each administration group is coughed, stridulated, gong sound begin after 3 days to alleviate in administration, compare with negative control group, and less appearances spirit is depressed, stay the minimizing of standing, search for food, the dried symptom of foot.Except that dead 7 of small dose group, all the other each groups are dead less.The chicken that dies of illness is carried out pathological anatomy, serosity or cheesy exudate are arranged in trachea, bronchus, nasal meatus and the hole as a result.The enlargement of part chicken kidney, pale, ureter is full of urate, Maxingshigan injecta has the curative effect of highly significant to the chicken bronchitis disease, also bronchial smooth muscle had tangible relexation, reducing heat and dispersing lung-QI, the effect of relieving cough and asthma, and the toxin that pathogenic microorganism is produced has Detoxication.Through safety test research, show that said preparation is safe and reliable, have no side effect and other untoward reaction, said preparation convenient drug administration, curative effect are better than control drug to chicken bronchial treatment of diseases effect really.

Beneficial effect:

The Maxingshigan injecta flavor compatibility that the present invention makes is rigorous, and clear a surname lays equal stress on, and dispersing accompanied by descending is played hot cool lung qi dispersing altogether, and the merit of clearing heat and relieving dyspnea is used for the treatment of poultry lung-heat cough with asthma, and body is brimful of tears not to be understood, and cough with dyspnea nose out of breath is stir-fried before stewing etc.

The specific embodiment

Best embodiment of the present invention divides other scheme according to integral body, implements respectively, below by embodiment the present invention is specifically described:

Embodiment 1:

1, the key component of Maxingshigan injecta is formed, and counts by weight, and raw material is composed as follows: 500 parts in Gypsum Fibrosum, 500 parts in Herba Ephedrae, 500 parts of Semen Armeniacae Amarums, 500 parts in Radix Glycyrrhizae;

2. and by following processing step and technological parameter prepare:

With 500 parts in Gypsum Fibrosum, 500 parts in Herba Ephedrae, 500 parts of Semen Armeniacae Amarums, 500 parts in Radix Glycyrrhizae, the broken mix homogeneously of 4 flavor medicated powder; Add 4000 parts in water and decoct twice, for the first time, respectively decocted 2 hours for the second time, collecting decoction filters, concentrating under reduced pressure, and cooling adds ethanol and makes and contain the alcohol amount and reach 60%, stirs evenly, and cold preservation filters, and filtrate concentrates, and reclaims ethanol, cold preservation.Filter, filtrate adds activated carbon decolorizing, and cold preservation under agitation adds Tween 80, adds the injection water to 1000ml, embedding, and sterilization gets final product.

With the Maxingshigan injecta intramuscular injection of producing, suffer from 30 of bronchitic chickling, every injection 0.15ml, 2 times on the one, administration 6 days, 29 chickling recoveries from illness.

Embodiment 2:

1, the key component of Maxingshigan injecta is formed, and counts by weight, and raw material is composed as follows: 450 parts in Gypsum Fibrosum, 480 parts in Herba Ephedrae, 450 parts of Semen Armeniacae Amarums, 480 parts in Radix Glycyrrhizae;

2. and by following processing step and technological parameter prepare:

With 450 parts in Gypsum Fibrosum, 480 parts in Herba Ephedrae, 450 parts of Semen Armeniacae Amarums, 480 parts in Radix Glycyrrhizae, the broken mix homogeneously of 4 flavor medicated powder; Add 4000 parts in water and decoct twice, for the first time, respectively decocted 2 hours for the second time, collecting decoction filters, concentrating under reduced pressure, and cooling adds ethanol and makes and contain the alcohol amount and reach 60%, stirs evenly, and cold preservation filters, and filtrate concentrates, and reclaims ethanol, cold preservation.Filter, filtrate adds activated carbon decolorizing, and cold preservation under agitation adds Tween 80, adds the injection water to 1000ml, embedding, and sterilization gets final product.

With the Maxingshigan injecta intramuscular injection of producing, 30 of the chickling that suffers from an inflammation of the lungs, every injection 0.15ml, 2 times on the one, administration 6 days, 27 chickling recoveries from illness.

More than show and described ultimate principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; that describes in the foregoing description and the description just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.The claimed scope of the present invention is defined by appending claims and equivalent thereof.

Claims (3)

1. the key component of Maxingshigan injecta is formed, and it is characterized in that, and count by weight that raw material is composed as follows: Gypsum Fibrosum 400--600 part, Herba Ephedrae 400--600 part, Semen Armeniacae Amarum 400-600 part, Radix Glycyrrhizae 400-500 part.

2. Maxingshigan injecta according to claim 1 is characterized in that, the optimum weight proportioning is as follows: 500 parts in Gypsum Fibrosum, 500 parts in Herba Ephedrae, 500 parts of Semen Armeniacae Amarums, 500 parts in Radix Glycyrrhizae.

3. the preparation method of Maxingshigan injecta is characterized in that, and prepares by following processing step and technological parameter:

With Gypsum Fibrosum 400--600 part, Herba Ephedrae 400--600 part, Semen Armeniacae Amarum 400--600 part, Radix Glycyrrhizae 400-500 part; The broken mix homogeneously of 4 flavor medicated powder; Add water 3000--7000 part and decoct twice, for the first time, respectively decocted 2 hours for the second time, collecting decoction filters, concentrating under reduced pressure, and cooling adds ethanol and makes and contain the alcohol amount and reach 60%, stirs evenly, and cold preservation filters, and filtrate concentrates, and reclaims ethanol, cold preservation.Filter, filtrate adds activated carbon decolorizing, and cold preservation under agitation adds Tween 80, adds the injection water to 1000ml, embedding, and sterilization gets final product.