CN103582805B - 可缩放光谱检测和测量 - Google Patents

可缩放光谱检测和测量 Download PDF

Info

Publication number
CN103582805B
CN103582805B CN201280018232.9A CN201280018232A CN103582805B CN 103582805 B CN103582805 B CN 103582805B CN 201280018232 A CN201280018232 A CN 201280018232A CN 103582805 B CN103582805 B CN 103582805B
Authority
CN
China
Prior art keywords
light
sample
light source
image sensor
sensor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201280018232.9A
Other languages
English (en)
Chinese (zh)
Other versions
CN103582805A (zh
Inventor
D·特蕾西
P·L·斯托克斯
S·科伊阿尼
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bio Rad Laboratories Inc
Original Assignee
Gnubio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gnubio Inc filed Critical Gnubio Inc
Publication of CN103582805A publication Critical patent/CN103582805A/zh
Application granted granted Critical
Publication of CN103582805B publication Critical patent/CN103582805B/zh
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/42—Absorption spectrometry; Double beam spectrometry; Flicker spectrometry; Reflection spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02—Details
    • G01J3/0205—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
    • G01J3/0208—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows using focussing or collimating elements, e.g. lenses or mirrors; performing aberration correction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02—Details
    • G01J3/0205—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
    • G01J3/021—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows using plane or convex mirrors, parallel phase plates, or particular reflectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02—Details
    • G01J3/0205—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
    • G01J3/0216—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows using light concentrators or collectors or condensers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02—Details
    • G01J3/0205—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
    • G01J3/0248—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows using a sighting port, e.g. camera or human eye
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02—Details
    • G01J3/0291—Housings; Spectrometer accessories; Spatial arrangement of elements, e.g. folded path arrangements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02—Details
    • G01J3/06—Scanning arrangements arrangements for order-selection
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02—Details
    • G01J3/08—Beam switching arrangements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02—Details
    • G01J3/10—Arrangements of light sources specially adapted for spectrometry or colorimetry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/12—Generating the spectrum; Monochromators
    • G01J3/14—Generating the spectrum; Monochromators using refracting elements, e.g. prisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/12—Generating the spectrum; Monochromators
    • G01J3/18—Generating the spectrum; Monochromators using diffraction elements, e.g. grating
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/2803—Investigating the spectrum using photoelectric array detector
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G01J3/4406—Fluorescence spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging

Landscapes

  • Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Dispersion Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
CN201280018232.9A 2011-03-31 2012-03-27 可缩放光谱检测和测量 Expired - Fee Related CN103582805B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161469889P 2011-03-31 2011-03-31
US61/469,889 2011-03-31
PCT/US2012/030716 WO2012135201A1 (en) 2011-03-31 2012-03-27 Scalable spectroscopic detection and measurement

Publications (2)

Publication Number Publication Date
CN103582805A CN103582805A (zh) 2014-02-12
CN103582805B true CN103582805B (zh) 2016-08-10

Family

ID=46931881

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201280018232.9A Expired - Fee Related CN103582805B (zh) 2011-03-31 2012-03-27 可缩放光谱检测和测量

Country Status (8)

Country Link
US (1) US9228898B2 (https=)
EP (1) EP2691752A4 (https=)
JP (1) JP5986623B2 (https=)
CN (1) CN103582805B (https=)
AU (1) AU2012236748B2 (https=)
CA (1) CA2841425C (https=)
SG (1) SG193437A1 (https=)
WO (1) WO2012135201A1 (https=)

Families Citing this family (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9581549B2 (en) 2010-12-07 2017-02-28 Gnubio, Inc. Nucleic acid target detection using a detector, a probe and an inhibitor
AU2012236713B2 (en) 2011-03-30 2016-11-24 Bio-Rad Laboratories, Inc. Injection of multiple volumes into or out of droplets
US9816931B2 (en) 2011-03-31 2017-11-14 Bio-Rad Laboratories, Inc. Managing variation in spectroscopic intensity measurements through the use of a reference component
CA2841425C (en) 2011-03-31 2018-05-01 Gnubio, Inc. Scalable spectroscopic detection and measurement
CN104781386B (zh) 2012-09-12 2018-04-06 基纽拜奥股份有限公司 用于进行试验的集成微流体系统、方法和试剂盒
US9291503B2 (en) * 2012-11-08 2016-03-22 Hitachi, Ltd. Flow type single-particle spectrometer
US9592503B2 (en) 2013-01-25 2017-03-14 Gnubio, Inc. System and method for performing droplet inflation
US9766261B2 (en) 2013-05-29 2017-09-19 Bio-Rad Laboratories, Inc. Low cost optical high speed discrete measurement system
US9809851B2 (en) 2013-05-29 2017-11-07 Bio-Rad Laboratories, Inc. Systems and methods for sequencing in emulsion based microfluidics
EP3039119A4 (en) 2013-08-27 2017-04-05 GnuBIO, Inc. Microfluidic devices and methods of their use
US9555411B2 (en) 2013-09-30 2017-01-31 Gnubio, Inc. Microfluidic cartridge devices and methods of use and assembly
WO2015081102A1 (en) 2013-11-27 2015-06-04 Gnubio, Inc. Microfluidic droplet packing
US9869664B2 (en) * 2013-12-18 2018-01-16 Saudi Arabian Oil Company Method for non-intrusive measurement of low water content in oil
US9683792B2 (en) 2014-06-30 2017-06-20 Bio-Rad Laboratories, Inc. Floating thermal contact enabled PCR
GB201415238D0 (en) * 2014-08-28 2014-10-15 Renishaw Plc Spectroscopy apparatus
TWI544210B (zh) * 2014-10-21 2016-08-01 王基旆 可攜式電子裝置、結合該裝置之光譜儀及利用該裝置檢測物品品質之方法
CN107636472B (zh) 2014-11-06 2020-09-15 基纽拜奥股份有限公司 微滴速度检测
CN104483104B (zh) * 2014-12-25 2018-01-12 中国科学院半导体研究所 一种光电探测器光谱响应分析系统
EP3256850B1 (en) 2015-02-09 2025-07-09 Slingshot Biosciences, Inc. Hydrogel particles with tunable optical properties and methods for using the same
JP6713477B2 (ja) * 2015-03-06 2020-06-24 ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ 集光システムならびにその製造および使用方法
US10809250B2 (en) 2015-08-25 2020-10-20 Bio-Rad Laboratories, Inc. Digital immunoassay
US10386284B2 (en) * 2016-01-29 2019-08-20 JianFeng Zhang Device and method for measurement of dispersed objects using fluorescent and non-fluorescent imaging with laser
WO2017180704A1 (en) 2016-04-14 2017-10-19 Saudi Arabian Oil Company Opto-mechanical part for parabolic mirror fine rotation and on-axis linear positioning
US10048205B2 (en) 2016-04-14 2018-08-14 Saudi Arabian Oil Company Characterizing petroleum product contamination using fluorescence signal
WO2017189603A1 (en) 2016-04-26 2017-11-02 Saudi Arabian Oil Company Characterizing lubricant oil degradation using fluorescence signals
CN106383083B (zh) * 2016-11-01 2018-10-16 北京信息科技大学 流式细胞仪光谱重叠信号数字处理方法
JP6784603B2 (ja) * 2017-01-05 2020-11-11 東レエンジニアリング株式会社 分光測定方法および分光測定装置
EP3747985A4 (en) * 2018-02-01 2021-10-27 Agc Inc. CELL CULTURE CONTAINER
WO2020142902A1 (zh) 2019-01-08 2020-07-16 京东方科技集团股份有限公司 流体检测面板和流体检测装置
CN109632660B (zh) * 2019-01-17 2022-04-05 京东方科技集团股份有限公司 流体检测面板
AU2020361681A1 (en) 2019-10-10 2022-05-05 1859, Inc. Methods and systems for microfluidic screening
JP7759328B2 (ja) 2020-01-24 2025-10-23 スリングショット バイオサイエンシーズ， インコーポレイテッド 細胞様較正粒子のための組成物および方法
CA3177834A1 (en) 2020-05-04 2021-11-11 Jeffrey Kim Compositions and methods for passive optical barcoding for multiplexed assays
WO2022140291A1 (en) 2020-12-21 2022-06-30 Singular Genomics Systems, Inc. Systems and methods for multicolor imaging
CN113155287B (zh) * 2021-04-27 2022-10-21 江苏汇鼎光学眼镜有限公司 一种眼镜片测色装置
JP2024539956A (ja) 2021-10-29 2024-10-31 スリングショット バイオサイエンシーズ， インコーポレイテッド フィーダー細胞としておよび合成抗原提示細胞としてのヒドロゲル粒子
US12259377B2 (en) 2022-04-05 2025-03-25 Saudi Arabian Oil Company Detecting hydrocarbon fuels in lubrication oils
CN119907921A (zh) 2022-05-05 2025-04-29 弹弓生物科学公司 用于血液学的作为红细胞模拟物的工程化颗粒和含有工程化颗粒的组合物
US12216041B2 (en) 2022-05-31 2025-02-04 Saudi Arabian Oil Company Method to detect diesel in fluid samples using partially dissolvable cuvettes
AU2023280490A1 (en) 2022-06-02 2024-12-05 Slingshot Biosciences, Inc. Apoptotic cell mimic
EP4608872A2 (en) 2022-10-26 2025-09-03 Slingshot Biosciences, Inc. Size-tunable synthetic particles with tunable optical properties and methods for using the same for immune cell activation
US12298266B2 (en) 2023-03-30 2025-05-13 Saudi Arabian Oil Company Changes in resistivity of mesh-patterned polymer thin films coated with conductive traces used to detect hydrocarbon fuels hidden in paraffin-based lubrication oils
US12546759B2 (en) 2023-03-30 2026-02-10 Saudi Arabian Oil Company Thin polymer film dissolved under tension to detect hydrocarbon fuels hidden in paraffin-based lubrication oils
US12517107B2 (en) 2023-04-11 2026-01-06 Saudi Arabian Oil Company Detecting hydrocarbon fuels in lubrication oils
WO2025049609A1 (en) 2023-08-29 2025-03-06 Slingshot Biosciences, Inc. Cd34 stem cell mimics
DE102023131464A1 (de) * 2023-11-13 2025-05-15 Fluidect Gmbh Detektionssystem und Verfahren zum Erfassen einer elektromagnetischen Emission von Mikropartikeln
US12584902B2 (en) 2023-12-10 2026-03-24 Instrumentation Laboratory Company Acoustic separation of a test sample

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5206701A (en) * 1991-09-20 1993-04-27 Amoco Corporation Apparatus for near-infrared spectrophotometric analysis
US20020009741A1 (en) * 1995-05-09 2002-01-24 Curagen Corporation Apparatus and method for the generation, separation, detection, and recognition of biopolymer fragments
US20040197816A1 (en) * 2000-04-06 2004-10-07 Quantum Dot Corporation Two-dimensional spectral imaging system
US20060066837A1 (en) * 1999-01-25 2006-03-30 Amnis Corporation Imaging and analyzing parameters of small moving objects such as cells
CN101023331A (zh) * 2004-06-30 2007-08-22 化学影像公司 生物细胞和其它对象的动态化学成像
CN101473201A (zh) * 2006-06-16 2009-07-01 瑞尼斯豪公司 光谱分析方法

Family Cites Families (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2975669A (en) * 1954-08-26 1961-03-21 Jarrell Ash Company Crossed dispersion photographic spectrometer
US5159199A (en) 1991-08-12 1992-10-27 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Integrated filter and detector array for spectral imaging
US5434629A (en) 1993-12-20 1995-07-18 Focus Automation Systems Inc. Real-time line scan processor
US5610771A (en) 1994-08-01 1997-03-11 Lockheed Missiles & Space Company, Inc. Non-deviating prism with continuously variable dispersion
US6982431B2 (en) * 1998-08-31 2006-01-03 Molecular Devices Corporation Sample analysis systems
US6043881A (en) * 1998-03-06 2000-03-28 Praxair Technology Inc Sample cell for gaseous emission spectroscopy
US6875973B2 (en) * 2000-08-25 2005-04-05 Amnis Corporation Auto focus for a flow imaging system
WO2002101339A2 (en) * 2000-10-12 2002-12-19 Amnis Corporation Imaging and analyzing parameters of small moving objects such as cells
CA2453390A1 (en) * 2001-07-25 2003-02-06 Applera Corporation Time-delay integration in electrophoretic detection systems
US6831688B2 (en) * 2002-04-08 2004-12-14 Recon/Optical, Inc. Multispectral or hyperspectral imaging system and method for tactical reconnaissance
WO2004081549A1 (en) 2003-03-11 2004-09-23 Koninklijke Philips Electronics N.V. Spectroscopic analysis apparatus and method with excitation system and focus monitoring system
CN104069784B (zh) 2003-08-27 2017-01-11 哈佛大学 流体物种的电子控制
CA2504817A1 (en) 2004-04-21 2005-10-21 University Technologies International Inc. Synchronization of time delay and integration cameras
EP1844426A4 (en) * 2005-02-01 2016-09-07 Amnis Corp Blood analysis using a flow imaging cytometer
EP1866613A4 (en) * 2005-04-08 2009-06-03 Chemimage Corp System and method for chemical imaging of microarrays
JP2009536313A (ja) 2006-01-11 2009-10-08 レインダンス テクノロジーズ， インコーポレイテッド ナノリアクターの形成および制御において使用するマイクロ流体デバイスおよび方法
US7815871B2 (en) * 2006-04-18 2010-10-19 Advanced Liquid Logic, Inc. Droplet microactuator system
ATE540750T1 (de) 2006-05-11 2012-01-15 Raindance Technologies Inc Mikrofluidische vorrichtung und verfahren
US8179526B2 (en) * 2007-01-25 2012-05-15 Renishaw Plc Spectroscopic apparatus with dispersive device for collecting sample data in synchronism with relative movement of a focus
JP5738597B2 (ja) 2007-12-21 2015-06-24 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸の配列決定のためのシステムおよび方法
US9358539B2 (en) 2008-05-16 2016-06-07 President And Fellows Of Harvard College Valves and other flow control in fluidic systems including microfluidic systems
US20110218123A1 (en) 2008-09-19 2011-09-08 President And Fellows Of Harvard College Creation of libraries of droplets and related species
WO2010080134A1 (en) 2008-12-19 2010-07-15 President And Fellows Of Harvard College Particle-assisted nucleic acid sequencing
US8047829B1 (en) 2009-01-26 2011-11-01 Sandia Corporation Method for forming polymerized microfluidic devices
EP3415235B1 (en) 2009-03-23 2025-11-12 Bio-Rad Laboratories, Inc. Manipulation of microfluidic droplets
JP5841937B2 (ja) 2009-06-26 2016-01-13 プレジデント アンド フェローズ オブ ハーバード カレッジ 流体注入
CN102648053B (zh) 2009-10-27 2016-04-27 哈佛学院院长等 液滴生成技术
CA2789425C (en) 2010-02-12 2020-04-28 Raindance Technologies, Inc. Digital analyte analysis with polymerase error correction
US9581549B2 (en) 2010-12-07 2017-02-28 Gnubio, Inc. Nucleic acid target detection using a detector, a probe and an inhibitor
EP3859011A1 (en) 2011-02-11 2021-08-04 Bio-Rad Laboratories, Inc. Methods for forming mixed droplets
EP3736281A1 (en) 2011-02-18 2020-11-11 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
AU2012236713B2 (en) 2011-03-30 2016-11-24 Bio-Rad Laboratories, Inc. Injection of multiple volumes into or out of droplets
US9816931B2 (en) 2011-03-31 2017-11-14 Bio-Rad Laboratories, Inc. Managing variation in spectroscopic intensity measurements through the use of a reference component
CA2841425C (en) 2011-03-31 2018-05-01 Gnubio, Inc. Scalable spectroscopic detection and measurement
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
WO2013095737A2 (en) 2011-09-28 2013-06-27 President And Fellows Of Harvard College Systems and methods for droplet production and/or fluidic manipulation
WO2013122826A1 (en) 2012-02-14 2013-08-22 Gnubio, Inc. Cascaded addition of target specific universal adapters to nucleic acids
EP3524693A1 (en) 2012-04-30 2019-08-14 Raindance Technologies, Inc. Digital analyte analysis
CN104781386B (zh) 2012-09-12 2018-04-06 基纽拜奥股份有限公司 用于进行试验的集成微流体系统、方法和试剂盒
CN105050583A (zh) 2012-12-14 2015-11-11 基纽拜奥股份有限公司 在乳液滴中维持分子的不均匀浓度的方法
US9592503B2 (en) 2013-01-25 2017-03-14 Gnubio, Inc. System and method for performing droplet inflation
EP2989213B1 (en) 2013-04-26 2019-11-06 Bio-Rad Laboratories, Inc. A method for blocking polymerase extension of 3 prime dna ends by stem-loop structure
US9809851B2 (en) 2013-05-29 2017-11-07 Bio-Rad Laboratories, Inc. Systems and methods for sequencing in emulsion based microfluidics

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5206701A (en) * 1991-09-20 1993-04-27 Amoco Corporation Apparatus for near-infrared spectrophotometric analysis
US20020009741A1 (en) * 1995-05-09 2002-01-24 Curagen Corporation Apparatus and method for the generation, separation, detection, and recognition of biopolymer fragments
US20060066837A1 (en) * 1999-01-25 2006-03-30 Amnis Corporation Imaging and analyzing parameters of small moving objects such as cells
US20040197816A1 (en) * 2000-04-06 2004-10-07 Quantum Dot Corporation Two-dimensional spectral imaging system
CN101023331A (zh) * 2004-06-30 2007-08-22 化学影像公司 生物细胞和其它对象的动态化学成像
CN101473201A (zh) * 2006-06-16 2009-07-01 瑞尼斯豪公司 光谱分析方法

Also Published As

Publication number Publication date
EP2691752A1 (en) 2014-02-05
US20140198313A1 (en) 2014-07-17
JP2014512532A (ja) 2014-05-22
AU2012236748A1 (en) 2013-10-03
WO2012135201A1 (en) 2012-10-04
JP5986623B2 (ja) 2016-09-06
CA2841425A1 (en) 2012-10-04
CN103582805A (zh) 2014-02-12
AU2012236748B2 (en) 2015-11-26
CA2841425C (en) 2018-05-01
SG193437A1 (en) 2013-10-30
EP2691752A4 (en) 2014-09-17
US9228898B2 (en) 2016-01-05

Similar Documents

Publication Publication Date Title
CN103582805B (zh) 可缩放光谱检测和测量
US10012592B2 (en) Managing variation in spectroscopic intensity measurements through the use of a reference component
US8629981B2 (en) Analyzers with time variation based on color-coded spatial modulation
US8373860B2 (en) Transmitting/reflecting emanating light with time variation
CN107850537B (zh) 辐射载体及其在光学传感器中的使用
US20020146734A1 (en) Method and apparatus for labeling and analyzing cellular components
CN101300478A (zh) 分析多光谱光学探测系统
US20090273774A1 (en) Oil-immersion enhanced imaging flow cytometer
CN116438438A (zh) 用于基于流动的单颗粒和/或单分子分析的方法和设备
US10203283B2 (en) Fluorescent substance detection system
CN105050583A (zh) 在乳液滴中维持分子的不均匀浓度的方法
KR100483706B1 (ko) 레이저 유발 표면형광 검출 장치
JP5634677B2 (ja) 被検体発光経時変化装置及び方法
WO2014134508A1 (en) Monochromatic dot ensembles
AU2004215325B2 (en) Spectrophotometer
HK1125695A (zh) 分析多光谱光学探测系统

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
TR01 Transfer of patent right

Effective date of registration: 20170720

Address after: California, USA

Patentee after: BIO-RAD LABORATORIES, Inc.

Address before: Massachusetts, USA

Patentee before: Nubeo GmbH

TR01 Transfer of patent right
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20160810

CF01 Termination of patent right due to non-payment of annual fee