CN103571735A - Pipe wall biofilm growth simulation reactor - Google Patents
Pipe wall biofilm growth simulation reactor Download PDFInfo
- Publication number
- CN103571735A CN103571735A CN201210249593.6A CN201210249593A CN103571735A CN 103571735 A CN103571735 A CN 103571735A CN 201210249593 A CN201210249593 A CN 201210249593A CN 103571735 A CN103571735 A CN 103571735A
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- water
- pipe
- pipeline section
- delivery pipeline
- water delivery
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/06—Tubular
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/58—Reaction vessels connected in series or in parallel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/06—Plates; Walls; Drawers; Multilayer plates
Abstract
The invention relates to a pipe wall biofilm growth simulation reactor, which comprises a water inlet pipe, a water outlet pipe and reactor bodies. The water inlet pipe and the water outlet pipe are connected by a plurality of reactor bodies in series connection. Each reactor body is composed of water pipe sections, threaded caps and fillers. Both ends of the water pipes are provided with dismountable threaded ports, and are connected to the threaded caps through the threaded ports. The fillers are disposed in the water pipes. Water enters from the water inlet pipe, penetrates each reactor body in order, and flows out from the water outlet pipe. The fillers in the water pipe sections are taken out, the biofilms on the fillers are collected, and a water sample is collected at the water outlet pipe to perform analysis. Compared with the prior art, the pipe wall biofilm growth simulation reactor provided by the invention truly simulates the hydraulic conditions and nutritional status of actual pipes, at the same time increases the biofilm attachment area, so that operation of the biofilm collection process can be simple, and no destructive effect is generated to the pipe walls.
Description
Technical field
The present invention relates to analogue reactor, especially relate to a kind of tube wall biofilm development analogue reactor.
Background technology
At present, known feedwater piping simulator has two kinds of designs: acyclic design and cyclic design.Acyclic design system is essentially plug flow reactor, biodegradable organic along with the increase of pipeline distance continuously by microbial consumption.Cyclic design system can be idealized as continuous-stirring reactor internal system mixes completely, and effluent quality and internal system condition of water quality are basic identical.But the surface-area that two kinds of pipeline analogue reactors adhere to for microbial film is less, and the sampling of acyclic simulation pipe wall microbial film is inconvenient, and sampling process has certain destructiveness to tube wall simultaneously, is unfavorable for the long-term continuous and steady operation of simulator.And the hydraulics of circulating analog pipeline and water nutrition situation and actual pipe condition differ larger, referential is less.
Summary of the invention
Object of the present invention is exactly to provide the actual pipeline hydraulic condition of a kind of practical simulation, nutritional status in order to overcome the defect of above-mentioned prior art existence, increase microbial film bond area simultaneously, and make microbial film gatherer process simple to operate, pipe wall be there is no to the feedwater piping biofilm development analogue reactor of destruction.
Object of the present invention can be achieved through the following technical solutions:
A kind of tube wall biofilm development analogue reactor, comprise water inlet pipe, rising pipe and reactor body, between described water inlet pipe and rising pipe, connect a plurality of reactor bodies that are connected in series, described reactor body is by water delivery pipeline section, screw thread block and media assemblage, the two ends of described water delivery pipeline section are provided with dismountable threaded port, by threaded port connecting thread, block a shot, described filler is located at the inside of water delivery pipeline section;
Water enters from water inlet pipe, penetrates successively each reactor body, from rising pipe, flows out, the filler taking out in water delivery pipeline section gathers the microbial film on filler, and analyze at rising pipe place collection water sample, simulative tube road is desirably plug flow reactor, and waterpower and water quality environment situation are with actual seemingly pipeline-like.
Described reactor body is provided with 1~50.Together with the two ends of described water delivery pipeline section are all screwed in screw thread block, wherein first segment water delivery pipeline section is connected with water inlet pipe, the other end of first segment water delivery pipeline section is connected with second section water delivery pipeline section by bend pipe, the other end of second section water delivery pipeline section is connected with the 3rd joint water delivery pipeline section by bend pipe, by that analogy, hydraulic pipe hop count amount is determined by demand.
The diameter of described water delivery pipeline section is 20mm~40mm, preferably 20mm, 25mm, 32mm or 40mm; The length of described water delivery pipeline section is 200mm~500mm, preferably 400mm; The tubing of described water delivery pipeline section is selected from cast iron pipe, steel pipe, stainless steel tube or plastics tubing.The tubing of water delivery pipeline section, caliber, pipe range can regulate according to practical situation, water delivery pipeline section is for simulating waterpower and the water quality condition in actual pipeline, dismountable threaded port is all made at the two ends of every section of water delivery pipeline section, for being connected of blocking a shot with screw thread, while being convenient to make every pipe joint section sampling, freely dismantle.
The size of described screw thread block and the size of water delivery pipeline section match, and in described screw thread block, are provided with seal washer.
Filler, for biomembranous enrichment in water delivery pipeline section, is guaranteed to sample and can under destroying, carried out inner-walls of duct, and described filler is plastic filler, and described filler is provided with 10~20.
The annular plastics filler that the preferred diameter of described plastic filler is 10mm, be used for increasing microbial film bond area, the convenient required microbial film of more experiment that obtains, simultaneously from filler, obtain microbial film and can utilize ultrasonator or vortex vibrator vibration wash-out, obtain biomembranous process convenient fully and pipeline section is not had to destruction.
Before simulation conduit running, need carry out high-temperature sterilization to filler, guarantee that filling surface is aseptic, prevent from bringing miscellaneous bacteria into tubing system, guarantee that in microbial film, microorganism all belongs to microorganisms in water.
Described water inlet pipe is provided with flooding velocity variable valve and under meter, and flooding velocity variable valve and under meter not only make simulation of water quality feasible, and can control flow and flow velocity in hydraulic pipe section, can the actual ducted hydraulic condition of optimization simulation.
The feed-water end of described reactor body is provided with inlet valve, described inlet valve is closed at microbial film sampling process sectional, water in can emptying adjacent tubular segments, therefore can not affect the water environment in other pipeline sections, to its inside biofilm development, disturbs less.
Between described reactor body, by bridge piece, connect successively.
The caliber of described bridge piece is less than the caliber of water delivery pipeline section, described bridge piece preferred glass bend pipe, glass bend pipe not only can Real Time Observation hydraulic pipe in streamflow regime, and can investigate biofilm development changing features on pipe wall.
Compared with prior art, the present invention has the following advantages:
1, simulation pipeline is acyclic plug flow reactor, can the actual pipe interior waterpower of real simulation, water quality condition;
2, the plastic filler of placing is easy to the more microbial film of enrichment, gathers microbial film simple to operation from plastic filler simultaneously, and sampling process to inner-walls of duct without destruction;
3, water delivery pipeline section that can free assembly and disassembly makes a whole set of simulator more flexible, can according to requirement of experiment, freely change tubing, pipe range and the caliber of water delivery pipeline section, with the requirement of meeting spatial and research;
While 4, sampling, can dismantle flexibly water delivery pipeline section, being about to this section closes with the upper inlet valve of installing of next joint being connected, more convenient sampling water delivery pipeline section is unloaded, thereby taking out built-in filler is eluted in the microbial film of apposition growth on filler in physiological saline by ultrasonic concussion mode or vortex concussion mode, because inlet valve is closed, the water of sampling process in can other water delivery pipeline sections of emptying, can not affect the water surrounding of other pipeline sections, to its inside bacterium, disturbs less;
5, the microbial film in desirable desired number pipeline section in some cycles, and make a record, the filler of Amoxcillin can be installed afterwards for microorganic adhesion, for subsequent experimental is prepared, give certain growth time, the microbial film in growth cycle in desirable other pipeline sections, simulator continuously-running.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention is described in detail.
Embodiment 1
A kind of tube wall biofilm development analogue reactor, structure as shown in Figure 1, comprise water inlet pipe 1, rising pipe 9 and reactor body, between water inlet pipe 1 and rising pipe 9, connect a plurality of reactor bodies that are connected in series, reactor body is comprised of water delivery pipeline section 6, screw thread block 5 and filler 7, the two ends of water delivery pipeline section 6 are provided with dismountable threaded port, and by threaded port connecting thread block 5, filler 7 is located at the inside of water delivery pipeline section 6;
Water enters from water inlet pipe 1, penetrate successively each reactor body, from rising pipe 9, flow out, take out the filler 7 in water delivery pipeline section 6, gather the microbial film on filler 7, and analyze at rising pipe 9 places' collection water samples, simulative tube road is desirably plug flow reactor, and waterpower and water quality environment situation are with actual seemingly pipeline-like.
Reactor body can be provided with 1~50, and in the present embodiment, the number of reactor body is 20.Together with the two ends of water delivery pipeline section 6 are all screwed in screw thread block 5, wherein first segment water delivery pipeline section 6 is connected with water inlet pipe 1, the other end of first segment water delivery pipeline section 6 is connected with second section water delivery pipeline section 6 by bend pipe, the other end of second section water delivery pipeline section 6 is connected with the 3rd joint water delivery pipeline section 6 by bend pipe, by that analogy, water delivery pipeline section 6 quantity are determined by demand.
The diameter of water delivery pipeline section 6 is 20mm~40mm, in the present embodiment, the diameter of water delivery pipeline section 6 is chosen respectively 20mm, 25mm, 32mm and 40mm, the length of water delivery pipeline section 6 is 200mm~500mm, in the present embodiment, the length of water delivery pipeline section 6 is 400mm, and the tubing of water delivery pipeline section 6 is selected from cast iron pipe, steel pipe, stainless steel tube or plastics tubing, and in the present embodiment, the tubing of water delivery pipeline section 6 is plastics tubing.The tubing of water delivery pipeline section 6, caliber, pipe range can regulate according to practical situation, water delivery pipeline section 6 is for simulating waterpower and the water quality condition in actual pipeline, dismountable threaded port is all made at the two ends of every section of water delivery pipeline section 6, for with being connected of screw thread block 5, while being convenient to make every pipe joint section sampling, freely dismantle.The size of screw thread block 5 and the size of water delivery pipeline section 6 match, and in screw thread block 5, are provided with seal washer.Filler 7, for the interior biomembranous enrichment of water delivery pipeline section 6, is guaranteed to sample and can under destroying, carried out inner-walls of duct, and filler 7 is provided with 10~20.The annular plastics filler 7 that plastic filler 7 is 10mm for diameter, be used for increasing microbial film bond area, the convenient required microbial film of more experiment that obtains, simultaneously from filler 7, obtain microbial film and can utilize ultrasonator or vortex vibrator vibration wash-out, obtain biomembranous process convenient fully and pipeline section is not had to destruction.
Before simulation conduit running, need carry out high-temperature sterilization to filler 7, guarantee filler 7 surface sterile, prevent from bringing miscellaneous bacteria into tubing system, guarantee that in microbial film, microorganism all belongs to microorganisms in water.
Water inlet pipe 1 is provided with flooding velocity variable valve 2 and under meter 3, and flooding velocity variable valve 2 and under meter 3 not only make simulation of water quality feasible, and can control flow and flow velocity in hydraulic pipe section 6, can the actual ducted hydraulic condition of optimization simulation.
The feed-water end of reactor body is provided with inlet valve 4, and inlet valve 4 is closed at microbial film sampling process sectional, and the water in can emptying adjacent tubular segments, therefore can not affect the water environment in other pipeline sections, to its inside biofilm development, disturbs less.
Between reactor body, by bridge piece 8, connect successively.The caliber of bridge piece 8 is less than the caliber of water delivery pipeline section 6, and bridge piece 8 is glass bend pipe, glass bend pipe not only can Real Time Observation hydraulic pipe in streamflow regime, and can investigate biofilm development changing features on pipe wall.
A biofilm development analogue reactor, structure as shown in Figure 1.Water inlet pipe 1 is connected with water tap, is installed into Water flow adjusting valve 2 on water inlet pipe, under meter 3.By screw thread, block a shot and 5 water inlet pipe is connected with water delivery pipeline section 6, in water inlet one end of pipeline section, inlet valve 4 is installed.The inner filler 7 of filling of water delivery pipeline section 6, the other end covers tightly sealing by screw thread block 5 again.Two adjacent screw thread blocks 5 are connected by bridge piece 8, can be made into one.By screw thread block 5, connect next pipe joint section again, the inner same plastic filler that loads equivalent amount of pipeline section, assembling by that analogy.Final stage pipeline section is blocked a shot and 5 is connected with rising pipe 9 by screw thread, and rising pipe 9 can be blocked a shot and 5 make one with screw thread.
The caliber of bridge piece 8 is less than the caliber of water delivery pipeline section 6, can prevent that pipeline section inner stuffing from leaking.In pipe thread block 5, seal washer is housed, can prevents that the interior current of water delivery pipeline section 6 from not leaking.The inlet valve 4 of installing at water delivery pipeline section 6 feed-water ends can be closed when sampling, and the pipeline section water that makes to be connected does not leak outside, and assurance sampling process is not disturbed the water body environment in other pipeline sections, makes microbial film continue growth.
On water inlet pipe 1, be installed into Water flow adjusting valve 2 and can regulate as required flooding velocity, by under meter 3, observe fluctuations in discharge, thereby make there is stable flow velocity in pipeline, control waterpower adjustment.
Before simulation conduit running, need carry out high-temperature sterilization to filler 7, guarantee filler 7 surface sterile, prevent from bringing miscellaneous bacteria into tubing system, guarantee that in microbial film, microorganism all belongs to microorganisms in water.
While gathering microbial film, the filler in a joint water delivery pipeline section 67 is taken out, according to experiment, need to take out the filler in more piece pipeline section.The filler of taking-up is placed in to the physiological saline of sterilizing, can microbial film be departed from physiological saline, to carry out follow-up study experiment by ultrasonator or vortex vibrator.
Water sample can gather at rising pipe 9 places, can to water sample, carry out the detection of conventional index, is convenient to study the impact of the variation of conventional water-quality guideline on biofilm development.
Claims (10)
1. a tube wall biofilm development analogue reactor, it is characterized in that, comprise water inlet pipe, rising pipe and reactor body, between described water inlet pipe and rising pipe, connect a plurality of reactor bodies that are connected in series, described reactor body is by water delivery pipeline section, screw thread block and media assemblage, the two ends of described water delivery pipeline section are provided with dismountable threaded port, by threaded port connecting thread, block a shot, and described filler is located at the inside of water delivery pipeline section;
Water enters from water inlet pipe, penetrates successively each reactor body, from rising pipe, flows out, and the filler taking out in water delivery pipeline section gathers the microbial film on filler, and analyzes at rising pipe place collection water sample.
2. a kind of tube wall biofilm development analogue reactor according to claim 1, is characterized in that, described reactor body is provided with 1~50.
3. a kind of tube wall biofilm development analogue reactor according to claim 1, is characterized in that, the diameter of described water delivery pipeline section is 20mm~40mm, preferably 20mm, 25mm, 32mm or 40mm; The length of described water delivery pipeline section is 200mm~500mm, preferably 400mm; The tubing of described water delivery pipeline section is selected from cast iron pipe, steel pipe, stainless steel tube or plastics tubing.
4. a kind of tube wall biofilm development analogue reactor according to claim 1, is characterized in that, the size of described screw thread block and the size of water delivery pipeline section match, and in described screw thread block, is provided with seal washer.
5. a kind of tube wall biofilm development analogue reactor according to claim 1, is characterized in that, described filler is plastic filler, and described filler is provided with 10~20.
6. a kind of tube wall biofilm development analogue reactor according to claim 5, is characterized in that the annular plastics filler that the preferred diameter of described plastic filler is 10mm.
7. a kind of tube wall biofilm development analogue reactor according to claim 1, is characterized in that, described water inlet pipe is provided with flooding velocity variable valve and under meter.
8. a kind of tube wall biofilm development analogue reactor according to claim 1, is characterized in that, the feed-water end of described reactor body is provided with inlet valve.
9. a kind of tube wall biofilm development analogue reactor according to claim 1, is characterized in that, between described reactor body, by bridge piece, connects successively.
10. a kind of tube wall biofilm development analogue reactor according to claim 9, is characterized in that, the caliber of described bridge piece is less than the caliber of water delivery pipeline section, described bridge piece preferred glass bend pipe.
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CN201210249593.6A CN103571735B (en) | 2012-07-18 | 2012-07-18 | A kind of tube wall biofilm development analogue reactor |
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CN201210249593.6A CN103571735B (en) | 2012-07-18 | 2012-07-18 | A kind of tube wall biofilm development analogue reactor |
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CN103571735B CN103571735B (en) | 2015-07-29 |
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103937660A (en) * | 2014-04-28 | 2014-07-23 | 同济大学 | Bench-scale device of water supply pipe network microbiological test |
CN111785117A (en) * | 2020-07-17 | 2020-10-16 | 盐城工学院 | Simulation experiment device and experiment method for attaching biological membrane to multi-material water delivery pipeline |
CN111826275A (en) * | 2020-07-17 | 2020-10-27 | 盐城工学院 | Simulation experiment device and experiment method for growth of biological membrane on pipe wall of long water conveying pipeline |
CN112978901A (en) * | 2021-03-02 | 2021-06-18 | 南开大学 | Reactor and method for in-situ culture of pipeline biofilm |
CN113956965A (en) * | 2021-09-13 | 2022-01-21 | 北京大学口腔医学院 | Device for simulating waterway of oral cavity comprehensive treatment table and biofilm formation method |
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CN101053705A (en) * | 2007-05-24 | 2007-10-17 | 江苏汉邦科技有限公司 | Five-zoning simulated movable bed chromatography device |
CN201313853Y (en) * | 2008-11-10 | 2009-09-23 | 重庆大学 | Biofilm reaction equipment for wastewater treatment |
CN102020392A (en) * | 2009-09-17 | 2011-04-20 | 朱华土 | Dyeing reclaimed water recycling process and equipment |
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2012
- 2012-07-18 CN CN201210249593.6A patent/CN103571735B/en not_active Expired - Fee Related
Patent Citations (3)
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CN101053705A (en) * | 2007-05-24 | 2007-10-17 | 江苏汉邦科技有限公司 | Five-zoning simulated movable bed chromatography device |
CN201313853Y (en) * | 2008-11-10 | 2009-09-23 | 重庆大学 | Biofilm reaction equipment for wastewater treatment |
CN102020392A (en) * | 2009-09-17 | 2011-04-20 | 朱华土 | Dyeing reclaimed water recycling process and equipment |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103937660A (en) * | 2014-04-28 | 2014-07-23 | 同济大学 | Bench-scale device of water supply pipe network microbiological test |
CN103937660B (en) * | 2014-04-28 | 2016-02-17 | 同济大学 | A kind of water supply network microbiological test small testing device |
CN111785117A (en) * | 2020-07-17 | 2020-10-16 | 盐城工学院 | Simulation experiment device and experiment method for attaching biological membrane to multi-material water delivery pipeline |
CN111826275A (en) * | 2020-07-17 | 2020-10-27 | 盐城工学院 | Simulation experiment device and experiment method for growth of biological membrane on pipe wall of long water conveying pipeline |
CN112978901A (en) * | 2021-03-02 | 2021-06-18 | 南开大学 | Reactor and method for in-situ culture of pipeline biofilm |
CN113956965A (en) * | 2021-09-13 | 2022-01-21 | 北京大学口腔医学院 | Device for simulating waterway of oral cavity comprehensive treatment table and biofilm formation method |
CN113956965B (en) * | 2021-09-13 | 2023-11-21 | 北京大学口腔医学院 | Device for simulating waterway of oral cavity comprehensive treatment table and biofilm forming method |
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