CN113956965A - Device for simulating waterway of oral cavity comprehensive treatment table and biofilm formation method - Google Patents
Device for simulating waterway of oral cavity comprehensive treatment table and biofilm formation method Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
Abstract
The invention provides a device for simulating a waterway of an oral comprehensive treatment table and a biofilm forming method, wherein the device comprises: a water inlet tank; a silica gel connecting pipe; an intelligent timing control box; a peristaltic pump; a straight-through joint made of polypropylene; a plastic waterway pipeline made of polytetrafluoroethylene; a three-way valve made of plastic materials and composed of terpolymers of acrylonitrile, butadiene and styrene; a water discharge tank; an experimental iron stand; 1ml syringe. The biofilm formation method comprises the following steps: s1, respectively introducing water from different water sources into the four water inlet tanks; s2, setting a biofilm observation interval by the small program end; and S3, operating the device. The invention solves the problem that the waterway pipeline of the actual oral comprehensive treatment table is not easy to detach so as to observe the growth cycle and the growth characteristic of the biological membrane, and provides a scientific research method and a disinfection basis for the waterway pollution problem of the oral comprehensive treatment table.
Description
Technical Field
The invention relates to the technical field of bacterial biofilm incubation systems, in particular to a device for simulating a waterway of an oral comprehensive treatment table and a biofilm formation method.
Background
The waterway pollution problem of the oral cavity comprehensive treatment table is ubiquitous in the world, and the health safety of patients and medical staff is seriously threatened. Years of research prove that the waterway of the oral comprehensive treatment table is seriously polluted, the waterway is not treated, and microorganisms in a water sample per milliliter are high up to 108CFU contains conditional pathogenic bacteria such as Pseudomonas aeruginosa, Legionella pneumophila, etc. When the pathogenic microorganisms are used for oral treatment, the pathogenic microorganisms can enter wounds of patients along with the rotation of the mobile phone and water mist sprayed by the three-purpose gun, and hospital infection is caused. The medicine has great influence on people with low immunity, such as pregnant women, children, patients with malignant tumor chemotherapy, people taking immunosuppressive agent and people with immunity deficiency, such as patients with acquired immunodeficiency syndrome. Researches find that the problem of waterway pollution of the oral comprehensive treatment table is difficult to effectively solve and is mainly influenced by a biological film in a waterway pipeline. The biomembrane in the waterway is different from the growth mode of free bacteria, is a complex thallus combination, is a microorganism membranous polymer adhered to the surface of a certain living or non-living carrier, and the main components of the polymer comprise protein, carbohydrate, lipid, extracellular DNA and the like. Because the diameter of the waterway pipeline of the dental chair is small, the liquid flows slowly and stops intermittently, and the pollution is caused by instantaneous negative pressure suck-back of a high-speed mobile phone, a biological film is very easily generated in the pipeline. Once the biofilm is formed, the anchorage cells secrete polysaccharide-protein complex with high viscoelasticity, so that the polysaccharide-protein complex is formedDifficult to remove in the waterway of the dental chair, and has the capacities of resisting dryness and self-protection. In the dental treatment process, the biomembrane adhered to the surface of the pipeline can continuously release conditional pathogenic bacteria to the water path, and the conditional pathogenic bacteria enter a treatment part along with water flow to cause cross infection of dental treatment, so that a serious person can cause shock. Therefore, effective removal of biofilm, particularly biofilm formation in the prevention and control waterway pipes, is critical to controlling the occurrence of infection caused by the waterway of the dental chair. However, the research on the biomembrane is limited due to the high cost and the inconvenience of disassembling the dental chair. The method for simulating the waterway of the oral cavity comprehensive treatment table to form the biofilm mainly takes a static culture method as a main method at present, the waterway of the oral cavity comprehensive treatment table is in a dynamic and static state and is continuously changed, and is influenced by the suck-back of a high-speed mobile phone, and the existing static culture method cannot be close to a real growth environment, so that the judgment is influenced.
At present, the research on the dynamic simulation device and method for the waterway biofilm formation of the oral comprehensive treatment table is almost not available, and the only patent about the waterway pipeline biofilm formation mainly focuses on the aspect of tap water supply.
1. The invention discloses a long water pipeline pipe wall biofilm growth simulation experiment device and an experiment method (application number: 202010691936.9). The invention discloses the long water pipeline pipe wall biofilm growth simulation experiment device, although the method can simulate a multi-stage water pipeline network; the method can effectively simulate the growth process of the biomembrane on the wall of the long-distance water pipeline under the conditions of different lengths and different residence times, but because the conditions in the dental treatment process are complex, the method cannot consider the conditions of waterway circulation working time, high-speed handset suck-back and the like, cannot well approach the clinical use conditions, and is different from the research object of the research.
2. The invention discloses a biofilm growth simulation device of a hanging-piece type water supply pipeline in an outer cylinder (application number: 202110108547.3). The device has good stability, more hanging-piece positions and capability of simulating pipeline flow state, but is different from the research in aspects of biofilm formation methods, research objects and the like.
3. The invention discloses a reactor for in-situ culturing a pipeline biological membrane and a method thereof (application number: 202110231901.1). The reactor for in-situ culturing the pipeline biological membrane and the method thereof are disclosed, and the device has short membrane culturing period and high efficiency, but is different from the research on the aspects of a biological membrane forming method, a research object and the like.
Therefore, the invention provides a device for simulating the waterway biofilm formation of an oral comprehensive treatment table and a method for forming the biofilm, aiming at obtaining the biofilm through the construction of the device for simulating the waterway biofilm formation of the oral comprehensive treatment table and the exploration of the method for forming the biofilm, and facilitating the subsequent biofilm formation characteristic research and the removal effect research of different disinfectants on the biofilm.
Disclosure of Invention
The invention aims to provide a device for simulating the formation of a water path biofilm of an oral cavity comprehensive treatment table and a method for forming the biofilm, wherein the flow rate of water flow in a plastic pipeline is intelligently controlled by a peristaltic pump, and meanwhile, liquid in the oral cavity when injected into a patient for dental treatment is injected by a three-way valve to simulate the suck-back of a high-speed mobile phone, so that the simulation device is ensured to be close to the biofilm formation process in an actual water path to the maximum extent; the PLC is used for acquiring the containing quantities of different water sources in the water inlet tank, the working state of the peristaltic pump and the generation time of the biological membrane in real time, and a sampling module is provided. The invention provides a reliable novel device and a method for producing a biological membrane, which solve the problem that the biological membrane is not easily obtained by detaching an actual water channel pipeline. The biological membrane obtained by the device and the method can be used for conveniently researching the removal effect of different disinfectants on the biological membrane, and has high practical value.
The technical scheme of the invention is as follows:
an apparatus for simulating a waterway of an oral complex treatment table, comprising:
a simulation device for simulating the waterway working process of the oral cavity comprehensive treatment table,
a small program end for acquiring the working state of the simulation equipment and the biofilm formation state through the mobile end,
wherein, simulation equipment includes:
a water supply module for simulating different water source water supply functions in treatment table water route, water supply module includes: four water inlet tanks for storing source water, wherein the bottom in each water inlet tank is provided with a pressure sensor, the four water inlet tanks are connected with a peristaltic pump through silica gel connecting pipes, the peristaltic pump is electrically connected with an intelligent timing control box, water outlets of the peristaltic pumps are respectively connected with four plastic waterway pipelines through straight-through joints, the other ends of the four plastic waterway pipelines are respectively connected with three-way valves, the four three-way valves are also respectively connected with a drainage tank and a 1mL injector for imitating a clinical high-speed mobile phone to suck back liquid in the oral cavity of a patient,
four plastics water route pipelines are close to tee bend valve door one end and realize the fixed of horizontal direction through experiment iron stand platform respectively, are equipped with the sampling module who is convenient for take a sample to the biomembrane on the plastics water route pipeline respectively, and the position fixation at one of them experiment iron stand platform middle part has control module, is equipped with in the control module: a PLC controller electrically connected with the intelligent timing control box and the four pressure sensors, a wireless networking submodule electrically connected with the PLC controller and used for connecting the small program end,
the small program end comprises: the system comprises a peristaltic pump state module, a time period setting module, a prompting module and a biomembrane state recording module, wherein the peristaltic pump state module is used for acquiring and displaying the working time and the working state of a peristaltic pump at regular time, the time period setting module is used for setting a biomembrane sampling observation time interval, the prompting module is used for prompting a user to check the biomembrane state of a water inlet tank, the peristaltic pump or different sampling times through ringing or vibration, and the biomembrane state recording module is used for recording the sampling biomembrane state of each time interval.
Further, the prompt module includes: the peristaltic pump state prompting submodule is used for prompting a user to check and confirm the working state of the peristaltic pump, the biomembrane state prompting submodule is used for setting a time interval according to a time period setting module to prompt the user, the injector prompting submodule is used for prompting the user to sample through a 1mL injector at the time interval of 30min, and the user of the mobile phone end supporting device monitors the simulation equipment in real time and is suitable for more application scenes.
Further, the sampling module includes: cutting second plastics water route pipeline so that the sampling area of turning over the internal surface, sampling area a week is equipped with the sealing washer that is used for preventing to leak, and sampling area one side is connected with the fixture block, and second plastics water route pipeline corresponds the position with the fixture block and is equipped with the draw-in groove, and the design of sampling module is convenient for the device user to the acquisition of biofilm formation state in different time quantum for the device user can acquire the data of biofilm more, convenient to use.
Furthermore, the front surface of the control module is provided with a touch display screen for displaying the type and the residual using amount of the water source arranged in each water inlet tank, recording and displaying the working state and the working time of the peristaltic pump, calculating and displaying the biofilm formation time and calculating and displaying the biofilm sampling times, so that a device user can conveniently observe the state of the simulation equipment.
Furthermore, the bottom end of the water inlet tank is higher than the top of the water discharge tank, so that water flow in the plastic water channel pipeline can be kept in a stagnation state conveniently, and generation of a biological film is facilitated.
Preferably, plastics waterway pipeline is made by the plastics material of polytetrafluoroethylene material, and diameter 3 x 2mm keeps unanimous with actual oral cavity comprehensive treatment platform waterway pipeline material diameter for analog device can accord with actual conditions as far as possible, and plastics waterway pipeline carries out the u type through the experiment iron stand platform and fixes, highly presses close to clinical waterway pipeline's the state of placing.
Preferably, the water sources of the four water inlet tanks are respectively: purified water, domestic tap water, water and sterile water after being processed by a central water supply system, and various water qualities can provide more data for the biofilm formation process, so that the research of a device user is facilitated.
The invention also provides a biofilm forming method for simulating the waterway of the oral comprehensive treatment table based on the device, which comprises the following steps:
s1, purified water, domestic tap water, water treated by a central water supply system and sterile water are respectively introduced into the four water inlet tanks;
s2, logging in the small program end by a user, and setting a sampling observation time interval of the biological membrane through a time period setting module;
s3 and simulation equipment is started, the PLC controls the peristaltic pump to work through the intelligent timing control box, in the working process of the peristaltic pump, a user samples and observes a biological membrane generated by the plastic water channel pipeline through the sampling module according to a set time interval, the observation result is recorded through the biological membrane state recording module, and after observation is finished, all the plastic water channel pipelines are replaced.
Further preferably, the specific working process of the simulation device in step S3 is as follows: the peristaltic pump simultaneously controls liquid in the four water inlet tanks to be sucked out through the silica gel connecting pipes and then flows into the four plastic water channel pipelines, the flow rate of water flow is 60ml/min, the working time is 8: 00-17: 00, the water flow is stopped for 10min after working every half hour, the non-working time keeps the water flow stopped state, and when the working is stopped every half hour, the liquid in 100ul oral cavities of a patient during dental treatment is injected from the three-way valve through a 1ml syringe to simulate the back suction of a high-speed hand phone.
Wherein, four water inlet tanks are 5L sealed and covered opaque glass bottles, the drain tank is 5L sealed and covered opaque glass bottles, the flow of the 24h waterway pipeline can be ensured, the bottom of the water inlet tank is 0.2m higher than the top of the drain tank, and the raw water is replaced once in 24 h.
The invention has the beneficial effects that:
1. the invention provides a device for simulating the formation of a waterway biomembrane of an oral comprehensive treatment table and a method for forming the biomembrane thereof, which adjust the flow rate of water flow by an intelligent control peristaltic pump so as to simulate the dynamic change state of the waterway of the oral comprehensive treatment table; injecting liquid into the oral cavity of a patient during dental treatment through a three-way valve to simulate high-speed handset suck-back; the U-shaped fixation of the plastic waterway pipeline is realized through the experimental iron support, the placement state of the clinical waterway pipeline is simulated, and the process that the simulation device is close to the biofilm formation in the actual waterway is ensured to the greatest extent;
2. according to the device for simulating the formation of the water channel biomembrane of the oral comprehensive treatment table and the method for forming the biomembrane thereof, provided by the invention, the containing amounts of different water sources in the water inlet tank, the working state of the peristaltic pump and the time for generating the biomembrane are obtained in real time through the PLC, a sampling module is provided, and the problem that the biomembrane is obtained because an actual water channel pipeline is not easy to detach is solved;
3. the device for simulating the waterway biomembrane formation of the oral comprehensive treatment table and the method for forming the biomembrane thereof can sample at any time in the biomembrane forming process, can obtain the biomembranes at different growth stages, and are convenient for the follow-up research on the biomembrane;
4. the device for simulating the formation of the water path biomembrane of the oral comprehensive treatment table and the method for forming the biomembrane thereof can directly place the disinfectant into the water inlet tank, thereby facilitating the subsequent research on the removal effect of the disinfectant on the biomembrane.
Drawings
FIG. 1 is a block diagram of the present invention;
FIG. 2 is a block diagram of a simulation apparatus of the present invention;
FIG. 3 is an enlarged detail view taken at A in FIG. 2;
FIG. 4 is a block diagram of the interior of the control module of the present invention;
FIG. 5 is a diagram of a prompt module architecture according to the present invention;
the device comprises a water inlet tank 1, a silica gel connecting pipe 2, an intelligent timing control box 3, a peristaltic pump 4, a straight-through joint 5, a plastic waterway pipeline 6, an experimental iron support 7, a three-way valve 8, a discharge water tank 9, an injector 10-1ml 10, a sampling module 11, a control module 12, a PLC 13, a wireless networking submodule 14, a sampling area 15, a sealing ring 16, a clamping block 17, a clamping block 18, a clamping groove 19, a touch display screen and a pressure sensor 20.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer, the present invention will be described in further detail with reference to the accompanying drawings, and it is apparent that the described embodiments are only a part of the embodiments of the present invention, not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The terminology used in the embodiments of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used in the examples of the present invention and the appended claims, the singular forms "a", "an", and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise, and "a plurality" typically includes at least two.
Example 1
As shown in fig. 1, the present embodiment is a device for simulating a waterway of an oral cavity complex treatment table, including:
a simulation device for simulating the waterway working process of the oral cavity comprehensive treatment table,
a small program end for acquiring the working state of the simulation equipment and the biofilm formation state through the mobile end,
as shown in fig. 2, wherein the simulation apparatus includes:
a water supply module for simulating different water source water supply functions in treatment table water route, water supply module includes: four water tanks 1 that intake that are used for depositing the source water, four water sources that intake tank 1 are respectively: purified water, domestic tap water, water after being treated by a central water supply system and sterile water, wherein the bottom in the water inlet tanks 1 is provided with a pressure sensor 20, the four water inlet tanks 1 are connected with peristaltic pumps 4 through silica gel connecting pipes 2, the peristaltic pumps 4 are electrically connected with an intelligent timing control box 3, the water outlets of the peristaltic pumps 4 are respectively connected with four plastic water channel pipelines 6 through straight-through joints 5, the other ends of the four plastic water channel pipelines 6 are respectively connected with three-way valves 8, the four three-way valves 8 are also respectively connected with a water discharge tank 9 and a 1mL injector 10 for simulating the oral liquid of a clinical high-speed mobile phone to suck back a patient, the bottom end of the water inlet tank 1 is higher than the top of the water discharge tank 9, and the plastic water channel 6 is made of polytetrafluoroethylene plastic,
four plastics waterway pipeline 6 are close to 8 one ends of three way valve and realize the fixed of horizontal direction through experiment iron stand platform 7 respectively, and the position fixation at 7 middle parts of one of them experiment iron stand platform has control module 12, is equipped with in control module 12: a PLC (programmable logic controller) 13 electrically connected with the intelligent timing control box 3 and the four pressure sensors 20, a wireless networking submodule 14 electrically connected with the PLC 13 and used for connecting a small program end, a touch display screen 19 used for displaying the type and the residual amount of a water source arranged in each water inlet box 1, recording and displaying the working state and the working time of the peristaltic pump 4, calculating and displaying the biofilm formation time and calculating and displaying the biofilm sampling times is arranged on the front surface of the control module 12,
the small program end comprises: the system comprises a peristaltic pump state module, a time period setting module, a prompting module and a biomembrane state recording module, wherein the peristaltic pump state module is used for acquiring and displaying the working time and working state of a peristaltic pump 4 at regular time, the time period setting module is used for setting a biomembrane sampling observation time interval, the prompting module is used for prompting a user to check the biomembrane state of a water inlet tank 1, the peristaltic pump 4 or different sampling times through ringing or vibration, and the biomembrane state recording module is used for recording the sampling biomembrane state of each time interval.
Example 2
The embodiment is a biofilm forming method of a device for simulating a waterway of an oral cavity comprehensive treatment table based on the device in the embodiment 1, and the method comprises the following steps:
s1, purified water, domestic tap water, water treated by a central water supply system and sterile water are respectively introduced into the four water inlet tanks 1;
s2, logging in the small program end by a user, and setting a sampling observation time interval of the biological membrane through a time period setting module;
s3, analog device equipment starts, PLC controller 13 controls peristaltic pump 4 through intelligent timing control box 3 and works, and the in-process of peristaltic pump 4 work, the user passes through sampling module 11 according to the time interval of setting for and takes a sample the observation to the biomembrane that plastics water route pipeline 6 generated to pass through biomembrane state record module with the observation result and carry out the record, after the observation, change all plastics water route pipelines 6.
The specific working process of the simulation device in step S3 is as follows: the peristaltic pump 4 simultaneously controls liquid in the four water inlet tanks 1 to be sucked out through the silica gel connecting pipes 2 and then flows into the four plastic waterway pipelines 6, the flow rate of water flow is 60ml/min, the working time is 8:00 to 17:00, the water flow is stopped for 10min after working every half hour, the non-working time keeps the water flow stopped state, and when the working stops every half hour, the liquid is immediately injected into 100ul oral cavity liquid of a patient during dental treatment through the three-way valve 8 through the 1ml injector 10 so as to simulate the back suction of a high-speed hand phone.
Example 3
As shown in fig. 5, the present embodiment is different from embodiment 1 in that:
the prompt module comprises: the peristaltic pump state prompting submodule is used for prompting a user to check and confirm the working state of the peristaltic pump 4, the biological membrane state prompting submodule is used for setting a time interval according to a time period setting module to prompt the user, and the injector prompting submodule is used for prompting the user to sample through the 1mL injector 10 at the time interval of 30 min.
Example 4
The embodiment is a biofilm formation method of the device for simulating the waterway of the oral comprehensive treatment table based on one of the devices in the embodiment 3, and the method comprises the following steps:
s1, purified water, domestic tap water, water treated by a central water supply system and sterile water are respectively introduced into the four water inlet tanks 1;
s2, logging in the small program end by a user, and setting a sampling observation time interval of the biological membrane through a time period setting module;
s3, starting the simulation equipment, controlling the peristaltic pump 4 to work by the PLC 13 through the intelligent timing control box 3, when the peristaltic pump 4 cannot be controlled by the PLC 13 through the intelligent timing control box 3 in the working process of the peristaltic pump 4, the state of the peristaltic pump 4 is transmitted to the small program end through the wireless networking submodule 14, the peristaltic pump state prompting submodule prompts a device user to overhaul the peristaltic pump 4, when the set biomembrane observation time is reached, the biomembrane state prompting submodule prompts a user to sample and observe the biomembrane through the sampling module 11, the user samples and observes the biomembrane generated by the plastic waterway pipeline 6 through the sampling module 11 according to a set time interval, and the observation result is recorded by the biomembrane state recording module, and all the plastic waterway pipelines 6 are replaced after the observation is finished.
The specific working process of the simulation device in step S3 is as follows: the peristaltic pump 4 simultaneously controls liquid in the four water inlet tanks 1 to be sucked out through the silica gel connecting pipes 2 and then flows into the four plastic water channel pipelines 6, the flow rate of water flow is 60ml/min, the working time is 8: 00-17: 00, the water flow stops for 10min after working every half hour, the non-working time keeps the water flow stop state, and when the work stops every half hour, the injector reminding submodule immediately prompts a user to inject 100ul of liquid in the oral cavity of a patient during dental treatment from the three-way valve 8 through the 1ml injector 10 so as to simulate the high-speed handset suck-back.
Example 5
As shown in fig. 3, the present embodiment is different from embodiment 3 in that:
the sampling module 11 includes: cutting plastics water route pipeline 6 so that turn over the sampling area 15 of internal surface, 15 a week in sampling area are equipped with the sealing washer 16 that is used for preventing to leak, and 15 one side in sampling area are connected with fixture block 17, and plastics water route pipeline 6 corresponds the position with fixture block 17 and is equipped with draw-in groove 18.
Example 6
The embodiment is a biofilm formation method of the device for simulating the waterway of the oral comprehensive treatment table based on the device of the embodiment 5, and the method comprises the following steps:
s1, purified water, domestic tap water, water treated by a central water supply system and sterile water are respectively introduced into the four water inlet tanks 1;
s2, logging in the small program end by a user, and setting a sampling observation time interval of the biological membrane through a time period setting module;
s3, starting the simulation equipment, controlling the peristaltic pump 4 to work by the PLC 13 through the intelligent timing control box 3, when the peristaltic pump 4 cannot be controlled by the PLC 13 through the intelligent timing control box 3 in the working process of the peristaltic pump 4, the state of the peristaltic pump 4 is transmitted to the small program end through the wireless networking submodule 14, the peristaltic pump state prompting submodule prompts a device user to overhaul the peristaltic pump 4, when the set biomembrane observation time is reached, the biomembrane state prompting submodule prompts a user to sample and observe the biomembrane through the sampling module 11, the user samples and observes the biomembrane generated by the plastic waterway pipeline 6 through the sampling module 11 according to a set time interval, and the observation result is recorded by the biomembrane state recording module, and all the plastic waterway pipelines 6 are replaced after the observation is finished.
The specific working process of the simulation device in step S3 is as follows: the peristaltic pump 4 simultaneously controls liquid in the four water inlet tanks 1 to be sucked out through the silica gel connecting pipes 2 and then flows into the four plastic water channel pipelines 6, the flow rate of water flow is 60ml/min, the working time is 8: 00-17: 00, the water flow stops for 10min after working every half hour, the non-working time keeps the water flow stop state, and when the work stops every half hour, the injector reminding submodule immediately prompts a user to inject 100ul of liquid in the oral cavity of a patient during dental treatment from the three-way valve 8 through the 1ml injector 10 so as to simulate the high-speed handset suck-back.
In step S3, the specific process of sampling and observing the biofilm is as follows: the user of the device lifts the sampling area 15 through the latch 17 to show the biofilm formed in the inner surface of the plastic waterway pipe 6, and takes out a part of the biofilm for observation.
Claims (9)
1. A device for simulating a waterway of an oral cavity comprehensive treatment table is characterized by comprising:
a simulation device for simulating the waterway working process of the oral cavity comprehensive treatment table,
a small program end for acquiring the working state of the simulation equipment and the biofilm formation state through a mobile end,
wherein the simulation apparatus comprises:
a water supply module for simulating different water source water supply functions in treatment table water route, water supply module includes: the four water inlet tanks (1) are used for storing source water, the bottom in each water inlet tank (1) is provided with a pressure sensor (20), the four water inlet tanks (1) are connected with peristaltic pumps (4) through silica gel connecting pipes (2), the peristaltic pumps (4) are electrically connected with an intelligent timing control box (3), the water outlets of the peristaltic pumps (4) are respectively connected with four plastic waterway pipelines (6) through straight-through joints (5), the other ends of the four plastic waterway pipelines (6) are respectively connected with three-way valves (8), the four three-way valves (8) are respectively connected with a water discharging tank (9) and a 1mL injector (10) used for simulating the liquid in the oral cavity of a clinical high-speed mobile phone to suck back a patient,
four plastics water route pipeline (6) are close to tee bend valve (8) one end and realize the fixed of horizontal direction through experiment iron stand platform (7) respectively, are equipped with sampling module (11) that are convenient for take a sample the observation to the biomembrane on plastics water route pipeline (6) respectively, one of them the position fixation at experiment iron stand platform (7) middle part has control module (12), be equipped with in control module (12): a PLC (13) electrically connected with the intelligent timing control box (3) and the four pressure sensors (20), and a wireless networking submodule (14) electrically connected with the PLC (13) and used for connecting the small program end,
the applet terminal includes: the system comprises a peristaltic pump state module, a time period setting module, a prompting module, a biofilm state recording module and a water residual amount monitoring module, wherein the peristaltic pump state module is used for acquiring and displaying the working time and the working state of a peristaltic pump (4), the time period setting module is used for setting biofilm sampling observation time intervals, the prompting module is used for prompting a user to check the water inlet tank (1), the peristaltic pump (4) or biofilm states at different sampling times through ringing or vibration, the biofilm state recording module is used for recording the sampling biofilm state at each time interval, and the water residual amount monitoring module is used for displaying and monitoring the water amount in the water inlet tank (1).
2. The device for simulating waterway of an oral complex treatment table of claim 1, wherein the prompt module comprises: the peristaltic pump state prompting submodule is used for prompting a user to check and confirm the working state of the peristaltic pump (4), the biomembrane state prompting submodule is used for prompting the user according to the time interval set by the time period setting module, and the injector prompting submodule is used for prompting the user to sample through the 1mL injector (10) by taking 30min as the time interval.
3. The device for simulating waterway of an oral complex treatment table according to claim 1, wherein the sampling module (11) comprises: will plastics water route pipeline (6) cut so that turn over sampling district (15) of internal surface, sampling district (15) a week is equipped with and is used for preventing sealing washer (16) that leak, sampling district (15) one side is connected with fixture block (17), plastics water route pipeline (6) with fixture block (17) correspond the position and are equipped with draw-in groove (18).
4. The device for simulating the waterway of the comprehensive oral treatment table according to claim 1, wherein the front surface of the control module (12) is provided with a touch display screen (19) for displaying the type and the residual using amount of the water source arranged in each water inlet tank (1), recording and displaying the working state and the working time of the peristaltic pump (4), calculating and displaying the biofilm formation time, and calculating and displaying the biofilm sampling times.
5. The device for simulating the waterway of an oral complex treatment table according to claim 1, wherein the bottom end of the water inlet tank (1) is higher than the top of the water discharge tank (9).
6. The device for simulating the waterway of an oral complex treatment table of claim 1, wherein the plastic waterway conduit (6) is made of a plastic material such as teflon.
7. The device for simulating the waterway of the oral complex treatment table according to claim 1, wherein the water sources of the four water inlet tanks (1) are respectively: purified water, domestic tap water, water treated by a central water supply system and sterile water.
8. The biofilm formation method of the device for simulating the waterway of an oral complex treatment table according to any one of claims 1 to 7, comprising the steps of:
s1, purified water, domestic tap water, water treated by a central water supply system and sterile water are respectively introduced into the four water inlet tanks (1);
s2, logging in the small program end by a user, and setting a sampling observation time interval of the biological membrane through a time period setting module;
s3, analog equipment starts, the PLC (13) controls the peristaltic pump (4) to work through the intelligent timing control box (3), the user samples and observes the biomembrane generated by the plastic water channel pipeline (6) through the sampling module (11) according to the set time interval in the working process of the peristaltic pump (4), the observation result is recorded through the biomembrane state recording module, and after the observation is finished, all the plastic water channel pipelines (6) are replaced.
9. The biofilm formation method for simulating the waterway of the oral complex treatment table as claimed in claim 8, wherein the specific working process of the simulation device in the step S3 is as follows: the peristaltic pump (4) simultaneously controls liquid in the four water inlet tanks (1) to be sucked out through the silica gel connecting pipes (2) and then flows into the four plastic water channel pipelines (6), the flow rate of water flow is 60ml/min, the working time is 8: 00-17: 00, the liquid stops for 10min after working every half hour, the non-working time keeps the state of water flow stopping, and when the working stops every half hour, the liquid is immediately injected into 100ul oral cavity liquid of a patient during dental treatment through the three-way valve (10) through the 1ml injector (12) so as to simulate the high-speed handset suck-back.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114732659A (en) * | 2022-04-01 | 2022-07-12 | 中国人民解放军联勤保障部队第九八0医院 | Method for preventing and treating microbial pollution of waterway pipeline of oral comprehensive treatment table |
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| CN113956965B (en) | 2023-11-21 |
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