CN103571735B - A kind of tube wall biofilm development analogue reactor - Google Patents
A kind of tube wall biofilm development analogue reactor Download PDFInfo
- Publication number
- CN103571735B CN103571735B CN201210249593.6A CN201210249593A CN103571735B CN 103571735 B CN103571735 B CN 103571735B CN 201210249593 A CN201210249593 A CN 201210249593A CN 103571735 B CN103571735 B CN 103571735B
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- pipeline section
- water
- delivery pipeline
- water delivery
- pipe
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/06—Tubular
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/58—Reaction vessels connected in series or in parallel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/06—Plates; Walls; Drawers; Multilayer plates
Abstract
The present invention relates to a kind of tube wall biofilm development analogue reactor, comprise water inlet pipe, rising pipe and reactor body, multiple reactor body be connected in series is connected between water inlet pipe and rising pipe, reactor body is blocked a shot by water delivery pipeline section, screw thread and filler forms, the two ends of water delivery pipeline section are provided with dismountable threaded port, blocked a shot by threaded port connecting thread, filler is located at the inside of water delivery pipeline section; Water enters from water inlet pipe, penetrates each reactor body successively, flows out from rising pipe, and the filler taken out in water delivery pipeline section gathers the microbial film on filler, and analyzes at water outlet pipe part collection water sample.Compared with prior art, the present invention simulates actual pipeline hydraulic condition, nutritional status conscientiously, increases microbial film bond area simultaneously, makes microbial film gatherer process simple to operate, to pipe wall without destruction.
Description
Technical field
The present invention relates to analogue reactor, especially relate to a kind of tube wall biofilm development analogue reactor.
Background technology
At present, known feedwater piping simulator has two kinds of designs: acyclic design and cyclic design.Acyclic design system is essentially plug flow reactor, biodegradable organic along with the increase of pipeline distance continuously by microbial consumption.Cyclic design system can be idealized as continuous-stirring reactor internal system and mix completely, and effluent quality is substantially identical with internal system condition of water quality.But two kinds of pipeline A reactors are less for the surface-area of microbial film attachment, and the sampling of acyclic simulation pipe wall microbial film is inconvenient, and sampling process has certain destructiveness to tube wall, is unfavorable for the long-term continuous and steady operation of simulator simultaneously.And the hydraulics of circulating analog pipeline and water nutrition situation differ comparatively large with actual pipe condition, referential is less.
Summary of the invention
Object of the present invention is exactly provide a kind of simulation conscientiously actual pipeline hydraulic condition, nutritional status to overcome defect that above-mentioned prior art exists, increase microbial film bond area simultaneously, and make microbial film gatherer process simple to operate, pipe wall be there is no to the feedwater piping biofilm development analogue reactor of destruction.
Object of the present invention can be achieved through the following technical solutions:
A kind of tube wall biofilm development analogue reactor, comprise water inlet pipe, rising pipe and reactor body, multiple reactor body be connected in series is connected between described water inlet pipe and rising pipe, described reactor body is blocked a shot by water delivery pipeline section, screw thread and filler forms, the two ends of described water delivery pipeline section are provided with dismountable threaded port, blocked a shot by threaded port connecting thread, described filler is located at the inside of water delivery pipeline section;
Water enters from water inlet pipe, penetrates each reactor body successively, flows out from rising pipe, the filler taken out in water delivery pipeline section gathers the microbial film on filler, and gather water sample in water outlet pipe part and analyze, simulative tube road is desirably plug flow reactor, waterpower and water quality environment situation and reality seemingly pipeline-like.
Described reactor body is provided with 1 ~ 50.Together with all blocking a shot with screw thread and be screwed in the two ends of described water delivery pipeline section, wherein first segment water delivery pipeline section is connected with water inlet pipe, the other end of first segment water delivery pipeline section is connected with second section water delivery pipeline section by bend pipe, the other end of second section water delivery pipeline section is connected with Section of three water delivery pipeline section by bend pipe, by that analogy, hydraulic pipe hop count amount is determined by demand.
The diameter of described water delivery pipeline section is 20mm ~ 40mm, preferred 20mm, 25mm, 32mm or 40mm; The length of described water delivery pipeline section is 200mm ~ 500mm, preferred 400mm; The tubing of described water delivery pipeline section is selected from cast iron pipe, steel pipe, stainless steel tube or plastics tubing.The tubing of water delivery pipeline section, caliber, pipe range can regulate according to practical situation, water delivery pipeline section is for simulating waterpower in actual pipeline and water quality condition, dismountable threaded port is all made at the two ends of every section of water delivery pipeline section, for the connection of blocking a shot with screw thread, be convenient to freely dismantle when every pipe joint section is sampled.
The described size of screw thread block and the size of water delivery pipeline section match, and are provided with seal washer in described screw thread block.
Filler is used for biomembranous enrichment in water delivery pipeline section, and guarantee that sampling can carried out under destroying inner-walls of duct, described filler is plastic filler, and described filler is provided with 10 ~ 20.
The preferred diameter of described plastic filler is the annular plastics filler of 10mm, for increasing microbial film bond area, the convenient microbial film obtained needed for more experiment, simultaneously obtain microbial film from filler and can utilize ultrasonator or turbula shaker vibration wash-out, obtain biomembranous process convenient fully and destruction is not had to pipeline section.
Before simulation conduit running, high-temperature sterilization need be carried out to filler, ensure that filling surface is aseptic, prevent from bringing miscellaneous bacteria into tubing system, ensure that in microbial film, microorganism all belongs to microorganisms in water.
Described water inlet pipe is provided with flooding velocity variable valve and under meter, and flooding velocity variable valve and under meter not only make simulation of water quality feasible, and the flow that can control in hydraulic pipe section and flow velocity, can the actual ducted hydraulic condition of optimization simulation.
The feed-water end of described reactor body is provided with inlet valve, described inlet valve is closed at microbial film sampling process sectional, can not water in emptying adjacent tubular segments, therefore can not affect the water environment in other pipeline sections, less to its Internal biological film growth interference.
Connected successively by bridge piece between described reactor body.
The caliber of described bridge piece is less than the caliber of water delivery pipeline section, described bridge piece preferred glass bend pipe, and glass bend pipe not only can streamflow regime in Real Time Observation hydraulic pipe, and can investigate biofilm development changing features on pipe wall.
Compared with prior art, the present invention has the following advantages:
1, simulating pipeline is acyclic plug flow reactor, can the actual pipe interior waterpower of real simulation, water quality condition;
2, the plastic filler placed is easy to the more microbial film of enrichment, and it is simple to operation simultaneously to gather microbial film from plastic filler, and sampling process to inner-walls of duct without destruction;
3, can the water delivery pipeline section of free assembly and disassembly make a whole set of simulator more flexible, experimentally can require freely to change the tubing of water delivery pipeline section, pipe range and caliber, with the requirement of meeting spatial and research;
4, water delivery pipeline section can be dismantled flexibly during sampling, by this section be connected next save the inlet valve installed and close, more convenient sampling water delivery pipeline section to be unloaded, take out built-in filler thus the microbial film of apposition growth on filler is eluted in physiological saline by ultrasonic vibration mode or vortex concussion mode, because inlet valve is closed, sampling process can not water in other water delivery pipeline sections of emptying, can not affect the water surrounding of other pipeline sections, less to its inside bacterium interference;
5, the microbial film in some cycles in desirable desired number pipeline section, and make a record, the filler of Amoxcillin can be installed afterwards for microorganic adhesion, for subsequent experimental prepares, give certain growth time, the microbial film in growth cycle in other pipeline sections desirable, simulator continuously-running.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention is described in detail.
Embodiment 1
A kind of tube wall biofilm development analogue reactor, structure as shown in Figure 1, comprise water inlet pipe 1, rising pipe 9 and reactor body, multiple reactor body be connected in series is connected between water inlet pipe 1 and rising pipe 9, reactor body is made up of water delivery pipeline section 6, screw thread block 5 and filler 7, the two ends of water delivery pipeline section 6 are provided with dismountable threaded port, and by threaded port connecting thread block 5, filler 7 is located at the inside of water delivery pipeline section 6;
Water enters from water inlet pipe 1, penetrate each reactor body successively, flow out from rising pipe 9, take out the filler 7 in water delivery pipeline section 6, gather the microbial film on filler 7, and gather water sample at rising pipe 9 place and analyze, simulative tube road is desirably plug flow reactor, waterpower and water quality environment situation and reality seemingly pipeline-like.
Reactor body can be provided with 1 ~ 50, and in the present embodiment, the number of reactor body is 20.The two ends of water delivery pipeline section 6 all block a shot with screw thread 5 be screwed in together with, wherein first segment water delivery pipeline section 6 is connected with water inlet pipe 1, the other end of first segment water delivery pipeline section 6 is connected with second section water delivery pipeline section 6 by bend pipe, the other end of second section water delivery pipeline section 6 is connected with Section of three water delivery pipeline section 6 by bend pipe, by that analogy, water delivery pipeline section 6 quantity is determined by demand.
The diameter of water delivery pipeline section 6 is 20mm ~ 40mm, in the present embodiment, the diameter of water delivery pipeline section 6 chooses 20mm, 25mm, 32mm and 40mm respectively, the length of water delivery pipeline section 6 is 200mm ~ 500mm, in the present embodiment, the length of water delivery pipeline section 6 is 400mm, and the tubing of water delivery pipeline section 6 is selected from cast iron pipe, steel pipe, stainless steel tube or plastics tubing, and in the present embodiment, the tubing of water delivery pipeline section 6 is plastics tubing.The tubing of water delivery pipeline section 6, caliber, pipe range can regulate according to practical situation, water delivery pipeline section 6 is for simulating waterpower in actual pipeline and water quality condition, dismountable threaded port is all made at the two ends of every section of water delivery pipeline section 6, for with screw thread block a shot 5 connection, be convenient to freely dismantle when every pipe joint section is sampled.The screw thread block size of 5 and the size of water delivery pipeline section 6 match, and are provided with seal washer in screw thread block 5.Filler 7 is for enrichment biomembranous in water delivery pipeline section 6, and guarantee that sampling can carried out under destroying inner-walls of duct, filler 7 is provided with 10 ~ 20.Plastic filler 7 for diameter be the annular plastics filler 7 of 10mm, for increasing microbial film bond area, the convenient microbial film obtained needed for more experiment, simultaneously obtain microbial film from filler 7 and can utilize ultrasonator or turbula shaker vibration wash-out, obtain biomembranous process convenient fully and destruction is not had to pipeline section.
Before simulation conduit running, high-temperature sterilization need be carried out to filler 7, ensure filler 7 surface sterile, prevent from bringing miscellaneous bacteria into tubing system, ensure that in microbial film, microorganism all belongs to microorganisms in water.
Water inlet pipe 1 is provided with flooding velocity variable valve 2 and under meter 3, and flooding velocity variable valve 2 and under meter 3 not only make simulation of water quality feasible, and the flow that can control in hydraulic pipe section 6 and flow velocity, can the actual ducted hydraulic condition of optimization simulation.
The feed-water end of reactor body is provided with inlet valve 4, and inlet valve 4 is closed at microbial film sampling process sectional, can not water in emptying adjacent tubular segments, therefore can not affect the water environment in other pipeline sections, less to its Internal biological film growth interference.
Connected successively by bridge piece 8 between reactor body.The caliber of bridge piece 8 is less than the caliber of water delivery pipeline section 6, and bridge piece 8 is glass bend pipe, and glass bend pipe not only can streamflow regime in Real Time Observation hydraulic pipe, and can investigate biofilm development changing features on pipe wall.
Embodiment 2
A kind of tube wall biofilm development analogue reactor, structure as shown in Figure 1.Water inlet pipe 1 is connected with water tap, water inlet pipe is installed into Water flow adjusting valve 2, under meter 3.By screw thread block 5, water inlet pipe is connected with water delivery pipeline section 6, in water inlet one end of pipeline section, inlet valve 4 is installed.Filler 7 is filled in water delivery pipeline section 6 inside, and the other end covers tightly sealing by screw thread block 5 again.Two adjacent screw thread blocks 5 are connected by bridge piece 8, can be made into one.Connect next pipe joint section by screw thread block 5 again, inner tube segment loads the plastic filler of equivalent amount equally, assembles by that analogy.Final stage pipeline section 5 to be connected with rising pipe 9 by screw thread block, and rising pipe 9 can be blocked a shot with screw thread and 5 to be made integrally.
The caliber of bridge piece 8 is less than the caliber of water delivery pipeline section 6, can prevent inner tube segment packing leaking.Pipe thread block 5, built with seal washer, can prevent current in water delivery pipeline section 6 from not leaking.Can close when sampling at the inlet valve 4 that water delivery pipeline section 6 feed-water end is installed, connected pipeline section water is not leaked outside, ensure that sampling process does not disturb the water body environment in other pipeline sections, make microbial film continued propagation.
Water inlet pipe 1 is installed into Water flow adjusting valve 2 and can regulates flooding velocity as required, observe fluctuations in discharge by under meter 3, thus make in pipeline, there is stable flow velocity, control waterpower adjustment.
Before simulation conduit running, high-temperature sterilization need be carried out to filler 7, ensure filler 7 surface sterile, prevent from bringing miscellaneous bacteria into tubing system, ensure that in microbial film, microorganism all belongs to microorganisms in water.
When gathering microbial film, the filler 7 in a joint water delivery pipeline section 6 is taken out, experimentally needs to take out the filler in more piece pipeline section.The filler of taking-up is placed in the physiological saline of sterilizing, by ultrasonator or turbula shaker, microbial film is departed from physiological saline, to carry out follow-up study experiment.
Water sample can gather at rising pipe 9 place, can carry out the detection of conventional index to water sample, is convenient to study the change of conventional water-quality guideline to the impact of biofilm development.
Claims (6)
1. a tube wall biofilm development analogue reactor, it is characterized in that, comprise water inlet pipe, rising pipe and reactor body, multiple reactor body be connected in series is connected between described water inlet pipe and rising pipe, described reactor body is blocked a shot by water delivery pipeline section, screw thread and filler forms, the two ends of described water delivery pipeline section are provided with dismountable threaded port, are blocked a shot by threaded port connecting thread, and described filler is located at the inside of water delivery pipeline section;
Described water inlet pipe is provided with flooding velocity variable valve and under meter;
The feed-water end of described reactor body is provided with inlet valve;
Described Fillers selection diameter is the annular plastics filler of 10mm;
Water enters from water inlet pipe, penetrates each reactor body successively, flows out from rising pipe, and the filler taken out in water delivery pipeline section gathers the microbial film on filler, and analyzes at water outlet pipe part collection water sample.
2. a kind of tube wall biofilm development analogue reactor according to claim 1, it is characterized in that, described reactor body is provided with 1 ~ 50.
3. a kind of tube wall biofilm development analogue reactor according to claim 1, is characterized in that, the diameter of described water delivery pipeline section is 20mm ~ 40mm; The length of described water delivery pipeline section is 200mm ~ 500mm; The tubing of described water delivery pipeline section is selected from cast iron pipe, steel pipe or plastics tubing.
4. a kind of tube wall biofilm development analogue reactor according to claim 1, is characterized in that, the described size of screw thread block and the size of water delivery pipeline section match, and is provided with seal washer in described screw thread block.
5. a kind of tube wall biofilm development analogue reactor according to claim 1, be is characterized in that, connected successively between described reactor body by bridge piece.
6. a kind of tube wall biofilm development analogue reactor according to claim 5, it is characterized in that, the caliber of described bridge piece is less than the caliber of water delivery pipeline section, and described bridge piece selects glass bend pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210249593.6A CN103571735B (en) | 2012-07-18 | 2012-07-18 | A kind of tube wall biofilm development analogue reactor |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210249593.6A CN103571735B (en) | 2012-07-18 | 2012-07-18 | A kind of tube wall biofilm development analogue reactor |
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| CN103571735A CN103571735A (en) | 2014-02-12 |
| CN103571735B true CN103571735B (en) | 2015-07-29 |
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| CN201210249593.6A Expired - Fee Related CN103571735B (en) | 2012-07-18 | 2012-07-18 | A kind of tube wall biofilm development analogue reactor |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103937660B (en) * | 2014-04-28 | 2016-02-17 | 同济大学 | A kind of water supply network microbiological test small testing device |
| CN111826275A (en) * | 2020-07-17 | 2020-10-27 | 盐城工学院 | Simulation experiment device and experiment method for growth of biological membrane on pipe wall of long water conveying pipeline |
| CN111785117A (en) * | 2020-07-17 | 2020-10-16 | 盐城工学院 | Simulation experiment device and experiment method for attaching biological membrane to multi-material water delivery pipeline |
| CN112978901A (en) * | 2021-03-02 | 2021-06-18 | 南开大学 | Reactor and method for in-situ culture of pipeline biofilm |
| CN113956965B (en) * | 2021-09-13 | 2023-11-21 | 北京大学口腔医学院 | Device for simulating waterway of oral cavity comprehensive treatment table and biofilm forming method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101053705A (en) * | 2007-05-24 | 2007-10-17 | 江苏汉邦科技有限公司 | Five-zoning simulated movable bed chromatography device |
| CN201313853Y (en) * | 2008-11-10 | 2009-09-23 | 重庆大学 | Biofilm reaction equipment for wastewater treatment |
| CN102020392A (en) * | 2009-09-17 | 2011-04-20 | 朱华土 | Dyeing reclaimed water recycling process and equipment |
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2012
- 2012-07-18 CN CN201210249593.6A patent/CN103571735B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101053705A (en) * | 2007-05-24 | 2007-10-17 | 江苏汉邦科技有限公司 | Five-zoning simulated movable bed chromatography device |
| CN201313853Y (en) * | 2008-11-10 | 2009-09-23 | 重庆大学 | Biofilm reaction equipment for wastewater treatment |
| CN102020392A (en) * | 2009-09-17 | 2011-04-20 | 朱华土 | Dyeing reclaimed water recycling process and equipment |
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Granted publication date: 20150729 Termination date: 20180718 |