CN103553817A - Efficient microbial fertilizer as well as preparation method thereof - Google Patents
Efficient microbial fertilizer as well as preparation method thereof Download PDFInfo
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- CN103553817A CN103553817A CN201310550387.3A CN201310550387A CN103553817A CN 103553817 A CN103553817 A CN 103553817A CN 201310550387 A CN201310550387 A CN 201310550387A CN 103553817 A CN103553817 A CN 103553817A
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Abstract
The invention discloses a microbial fertilizer as well as a preparation method thereof. The microbial fertilizer comprises bran, humic acid, black humic acid, fulvic acid, ulmic acid, molasses, sugarcane filter mud, tobacco powder, medical stone, nitrogen fixation strain, bentonite and ferment bacterium. The preparation method of the microbial fertilizer comprises the following steps: (1) adding the ferment bacterium into bran culture for culturing, drying the cultured bran under the normal temperature until moisture content is 10% for future use; (2) stirring; (3) fermenting the nitrogen fixation strain; (4) mixing the cultured bran, the material obtained by mixing in the step (2), the fermented nitrogen fixation strain, the black humic acid, the humic acid, the fulvic acid and the ulmic acid, adjusting a pH value to 8, controlling a temperature of incubator at 70 DEG C, shading and fermenting for 50 hours; (5) colleting bacteria; (6) sterilizing; and (7) pelletizing. The efficient microbial fertilizer disclosed by the invention is high in fertilizer effect, obvious in yield-increasing and quality-improving effect, reduces environmental pollution, and improves soil environment; and moreover, the preparation method is simple.
Description
technical field:
The present invention relates to a kind of microbial fertilizer and preparation method thereof, belong to bio-feritlizer technical field.
background technology:
In recent decades, in agriculture production, use in a large number the chemical substances such as chemical fertilizer, chemical pesticide, chemical herbicide.Make soil physical chemistry performance variation, soil compaction, vigor reduction, contaminate environment, crops quality declines, residual exceeding standard, and the utilization ratio reduced year by year of chemical fertilizer, agricultural chemicals, its major cause is exactly that soil with organic matter constantly reduces, can stimulate plant growth and the beneficial microorganism that can improve crop disease-resistant ability to reduce in a large number, change this present situation, just need in soil, add organic matter and beneficial microorganism, therefore microorganism fertilizer just arises at the historic moment, but existing microbial fertilizer fertilizer efficiency is low, increasing yield and improving quality DeGrain.
And enzymatic microorganism has extremely strong aerobic fermentation capacity of decomposition.Can decompose organic matter, remains of pesticide, fertilizer.Prevent the soil salinization, can dissolve the nutrition being cured in soil again, increase soil permeability and preserve moisture and fertility ability, kill pathogenic bacteria in soil, increase soil probiotics content, improve Soil structure, improve the quality and yield of farm crop.
summary of the invention:
The present invention seeks to, be used for making up the deficiencies in the prior art, and microbial fertilizer of a kind of high fertilizer efficiency, increasing yield and improving quality successful and preparation method thereof is provided, reduced environmental pollution, improved edatope, and preparation method is simple.
To achieve these goals, technical scheme of the present invention is as follows:
A kind of high fertilizer efficiency microbial fertilizer of the present invention, comprises wheat bran, humic acids, Ulmic acids, xanthohumic acid, ulmic acid, molasses, filter mud of sugarcane, tobacco powder, medical stone, fixed nitrogen bacterial classification, wilkinite, enzymatic microorganism; Each component concentration is respectively by massfraction: wheat bran 55-65 part, humic acids 18-25 part, Ulmic acids 10-18 part, xanthohumic acid 5-8 part, ulmic acid 16-22 part, molasses 30-50 part, filter mud of sugarcane 100-138 part, tobacco powder 50-75 part, medical stone 40-70 part, fixed nitrogen bacterial classification 20-30 part, wilkinite 35-65 part, enzymatic microorganism 25-45 part.
58 parts, above-mentioned wheat bran, 20 parts of humic acidss, 15 parts of Ulmic acidss, 7 parts of xanthohumic acids, 20 parts of ulmic acids, 40 parts, molasses, 128 parts of filter mud of sugarcanes, 60 parts, tobacco powder, 50 parts of medical stones, 25 parts of fixed nitrogen bacterial classifications, 50 parts of wilkinites, 35 parts of enzymatic microorganism.
The preparation method of the high fertilizer efficiency microbial fertilizer of the present invention, comprises following step:
(1) described enzymatic microorganism is added in wheat bran culture and cultivated, it is 10% standby that the wheat bran normal temperature after cultivating is dried in the air to water content, and the useful viable count of described enzymatic microorganism is 100～20,000,000,000/g;
(2) stir: send the molasses that prepare, filter mud of sugarcane, tobacco powder, medical stone, wilkinite to stirred pot the inside, under the uniform stirring speed turning, be uniformly mixed at per minute 50～55, temperature is controlled at 20～35 degree;
(3) fermentation fixed nitrogen bacterial classification: first, will proceed to sterilized liquid seed culture medium after described fixed nitrogen actication of culture, regulating PH is 6.5, cultivates 5 hours at 30 ℃; Then, by 8% inoculum size, proceed in the large tank substratum of sterilizing, in described large tank substratum, with the environment of 30 ℃, cultivate 5 hours;
(4) Secondary Fermentation: after the wheat bran after cultivating, material, the fixed nitrogen bacterial classification after fermentation, Ulmic acids, humic acids, xanthohumic acid, ulmic acid that step (2) is mixed to get are mixed, regulating pH value is 8, control incubator temperature 70 C, 100 ℃ of humidity, oxygen is appropriate, shading fermentation 50h;
(5) microorganism collection: by the overdue fermented product of above-mentioned fermentation, after pure bacterium experimental verification is without living contaminants, the centrifugal supernatant that goes, obtains concentrated wet thallus;
(6) sterilizing: wet thallus is heated to sterilizing in 3～4 hours at 100 ℃～120 ℃;
(7) granulation: the raw material that step (6) is obtained is sent tablets press to, the granular substance after granulator granulation, sends Temperature Setting to and is dry 25 minutes of the loft drier the inside of 140 ℃, obtains microbial fertilizer particle.
In step (3), the formula of aforesaid liquid seed culture medium comprises glucose, peptone and mineral substance.
In step (3), the formula of above-mentioned large tank substratum comprises sucrose, Semen Maydis powder, yeast extract paste, peptone and nutritive salt.
Wheat bran in fertilizer of the present invention can be bonded together the amine in compound listed above, amine surrogate, ammonium iron, thereby when being applied in soil, product of the present invention can make the nitrogenous compound forming in dividing produce the phenomenon slowly discharging, can not pollute because of loss water and soil, thereby improve edatope; Method disclosed by the invention is simple, energy-conserving and environment-protective, and the microorganism fertilizer fertilizer efficiency of producing is high, has the effect of increasing yield and improving quality clearly.
embodiment:
For technique means, creation characteristic that the present invention is realized, reach object and effect is easy to understand, further set forth the present invention.
Embodiment 1:
A kind of microbial fertilizer of the present invention, comprises wheat bran, humic acids, Ulmic acids, xanthohumic acid, ulmic acid, molasses, filter mud of sugarcane, tobacco powder, medical stone, fixed nitrogen bacterial classification, wilkinite, enzymatic microorganism; Each component concentration is respectively by massfraction: 55 parts, wheat bran, 18 parts of humic acidss, 10 parts of Ulmic acidss, 5 parts of xanthohumic acids, 16 parts of ulmic acids, 30 parts, molasses, 100 parts of filter mud of sugarcanes, 50 parts, tobacco powder, 40 parts of medical stones, 20 parts of fixed nitrogen bacterial classifications, 35 parts of wilkinites, 25 parts of enzymatic microorganism.
Wheat bran in fertilizer of the present invention can be bonded together the amine in compound listed above, amine surrogate, ammonium iron, thereby when being applied in soil, product of the present invention can make the nitrogenous compound forming in dividing produce the phenomenon slowly discharging, can not pollute because of loss water and soil, thereby improve edatope.
The preparation method of microbial fertilizer of the present invention, comprises following step:
(1) enzymatic microorganism is added in wheat bran culture and cultivated, it is 10% standby that the wheat bran normal temperature after cultivating is dried in the air to water content, and the useful viable count of described enzymatic microorganism is 10,000,000,000/g;
(2) stir: send the molasses that prepare, filter mud of sugarcane, tobacco powder, medical stone, wilkinite to stirred pot the inside, under the uniform stirring speed turning, be uniformly mixed at per minute 50, temperature is controlled at 20 degree;
(3) fermentation fixed nitrogen bacterial classification: first, will proceed to sterilized liquid seed culture medium after described fixed nitrogen actication of culture, regulating PH is 6.5, cultivates 5 hours at 30 ℃; Then, by 8% inoculum size, proceed in the large tank substratum of sterilizing, in described large tank substratum, with the environment of 30 ℃, cultivate 5 hours; The formula of liquid seed culture medium comprises glucose, peptone and mineral substance; The formula of large tank substratum comprises sucrose, Semen Maydis powder, yeast extract paste, peptone and nutritive salt.
(4) Secondary Fermentation: after the wheat bran after cultivating, material, the fixed nitrogen bacterial classification after fermentation, Ulmic acids, humic acids, xanthohumic acid, ulmic acid that step (2) is mixed to get are mixed, regulating pH value is 8, control incubator temperature 70 C, 100 ℃ of humidity, oxygen is appropriate, shading fermentation 50h;
(5) microorganism collection: by the overdue fermented product of above-mentioned fermentation, after pure bacterium experimental verification is without living contaminants, the centrifugal supernatant that goes, obtains concentrated wet thallus;
(6) sterilizing: wet thallus is heated to sterilizing in 3 hours at 100 ℃;
(7) granulation: the raw material that step (6) is obtained is sent tablets press to, the granular substance after granulator granulation, sends Temperature Setting to and is dry 25 minutes of the loft drier the inside of 140 ℃, obtains microbial fertilizer particle.
Method disclosed by the invention is simple, energy-conserving and environment-protective, and the microorganism fertilizer fertilizer efficiency of producing is high, has the effect of increasing yield and improving quality clearly.
Embodiment 2:
A kind of microbial fertilizer of the present invention, comprises wheat bran, humic acids, Ulmic acids, xanthohumic acid, ulmic acid, molasses, filter mud of sugarcane, tobacco powder, medical stone, fixed nitrogen bacterial classification, wilkinite, enzymatic microorganism; Each component concentration is respectively by massfraction: 65 parts, wheat bran, 25 parts of humic acidss, 18 parts of Ulmic acidss, 8 parts of xanthohumic acids, 22 parts of ulmic acids, 50 parts, molasses, 138 parts of filter mud of sugarcanes, 75 parts, tobacco powder, 70 parts of medical stones, 30 parts of fixed nitrogen bacterial classifications, 65 parts of wilkinites, 45 parts of enzymatic microorganism.
The preparation method of microbial fertilizer of the present invention, comprises following step:
(1) described enzymatic microorganism is added in wheat bran culture and cultivated, it is 10% standby that the wheat bran normal temperature after cultivating is dried in the air to water content, and the useful viable count of described enzymatic microorganism is 20,000,000,000/g;
(2) stir: send the molasses that prepare, filter mud of sugarcane, tobacco powder, medical stone, wilkinite to stirred pot the inside, under the uniform stirring speed turning, be uniformly mixed at per minute 55, temperature is controlled at 35 degree;
(3) fermentation fixed nitrogen bacterial classification: first, will proceed to sterilized liquid seed culture medium after described fixed nitrogen actication of culture, regulating PH is 6.5, cultivates 5 hours at 30 ℃; Then, by 8% inoculum size, proceed in the large tank substratum of sterilizing, in described large tank substratum, with the environment of 30 ℃, cultivate 5 hours; The formula of liquid seed culture medium comprises glucose, peptone and mineral substance; The formula of large tank substratum comprises sucrose, Semen Maydis powder, yeast extract paste, peptone and nutritive salt.
(4) Secondary Fermentation: after the wheat bran after cultivating, material, the fixed nitrogen bacterial classification after fermentation, Ulmic acids, humic acids, xanthohumic acid, ulmic acid that step (2) is mixed to get are mixed, regulating pH value is 8, control incubator temperature 70 C, 100 ℃ of humidity, oxygen is appropriate, shading fermentation 50h;
(5) microorganism collection: by the overdue fermented product of above-mentioned fermentation, after pure bacterium experimental verification is without living contaminants, the centrifugal supernatant that goes, obtains concentrated wet thallus;
(6) sterilizing: wet thallus is heated to sterilizing in 4 hours at 120 ℃;
(7) granulation: the raw material that step (6) is obtained is sent tablets press to, the granular substance after granulator granulation, sends Temperature Setting to and is dry 25 minutes of the loft drier the inside of 140 ℃, obtains microbial fertilizer particle.
More than show and described ultimate principle of the present invention and principal character and advantage of the present invention.The technician of the industry should understand; the present invention is not restricted to the described embodiments; that in above-described embodiment and specification sheets, describes just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.The claimed scope of the present invention is defined by appending claims and equivalent thereof
.
Claims (5)
1. a high fertilizer efficiency microbial fertilizer, is characterized in that, comprises wheat bran, humic acids, Ulmic acids, xanthohumic acid, ulmic acid, molasses, filter mud of sugarcane, tobacco powder, medical stone, fixed nitrogen bacterial classification, wilkinite, enzymatic microorganism;
Each component concentration is respectively by massfraction:
Wheat bran 55-65 part, humic acids 18-25 part, Ulmic acids 10-18 part, xanthohumic acid 5-8 part, ulmic acid 16-22 part, molasses 30-50 part, filter mud of sugarcane 100-138 part, tobacco powder 50-75 part, medical stone 40-70 part, fixed nitrogen bacterial classification 20-30 part, wilkinite 35-65 part, enzymatic microorganism 25-45 part.
2. high fertilizer efficiency microbial fertilizer according to claim 1, is characterized in that,
58 parts, described wheat bran, 20 parts of humic acidss, 15 parts of Ulmic acidss, 7 parts of xanthohumic acids, 20 parts of ulmic acids, 40 parts, molasses, 128 parts of filter mud of sugarcanes, 60 parts, tobacco powder, 50 parts of medical stones, 25 parts of fixed nitrogen bacterial classifications, 50 parts of wilkinites, 35 parts of enzymatic microorganism.
3. the preparation method of high fertilizer efficiency microbial fertilizer according to claim 1, is characterized in that, comprises following step:
(1) described enzymatic microorganism is added in wheat bran culture and cultivated, it is 10% standby that the wheat bran normal temperature after cultivating is dried in the air to water content, and the useful viable count of described enzymatic microorganism is 100～20,000,000,000/g;
(2) stir: send the molasses that prepare, filter mud of sugarcane, tobacco powder, medical stone, wilkinite to stirred pot the inside, under the uniform stirring speed turning, be uniformly mixed at per minute 50～55, temperature is controlled at 20～35 degree;
(3) fermentation fixed nitrogen bacterial classification: first, will proceed to sterilized liquid seed culture medium after described fixed nitrogen actication of culture, regulating PH is 6.5, cultivates 5 hours at 30 ℃; Then, by 8% inoculum size, proceed in the large tank substratum of sterilizing, in described large tank substratum, with the environment of 30 ℃, cultivate 5 hours;
(4) Secondary Fermentation: after the wheat bran after cultivating, material, the fixed nitrogen bacterial classification after fermentation, Ulmic acids, humic acids, xanthohumic acid, ulmic acid that step (2) is mixed to get are mixed, regulating pH value is 8, control incubator temperature 70 C, 100 ℃ of humidity, oxygen is appropriate, shading fermentation 50h;
(5) microorganism collection: by the overdue fermented product of above-mentioned fermentation, after pure bacterium experimental verification is without living contaminants, the centrifugal supernatant that goes, obtains concentrated wet thallus;
(6) sterilizing: wet thallus is heated to sterilizing in 3～4 hours at 100 ℃～120 ℃;
(7) granulation: the raw material that step (6) is obtained is sent tablets press to, the granular substance after granulator granulation, sends Temperature Setting to and is dry 25 minutes of the loft drier the inside of 140 ℃, obtains microbial fertilizer particle.
4. the preparation method of high fertilizer efficiency microbial fertilizer according to claim 3, is characterized in that,
In step (3), the formula of described liquid seed culture medium comprises glucose, peptone and mineral substance.
5. the preparation method of high fertilizer efficiency microbial fertilizer according to claim 4, is characterized in that,
In step (3), the formula of described large tank substratum comprises sucrose, Semen Maydis powder, yeast extract paste, peptone and nutritive salt.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103814649A (en) * | 2014-03-03 | 2014-05-28 | 江苏上田环境修复有限公司 | Method for improving farmland soil environment capacity |
| US10059632B2 (en) | 2015-11-05 | 2018-08-28 | Shandong Tralin Paper Co., Ltd. | Humic acid composition and application thereof |
| CN110386844A (en) * | 2019-07-25 | 2019-10-29 | 王晓雯 | A kind of fulvic acid microbial bacterial agent and preparation method thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103814649A (en) * | 2014-03-03 | 2014-05-28 | 江苏上田环境修复有限公司 | Method for improving farmland soil environment capacity |
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| CN110386844A (en) * | 2019-07-25 | 2019-10-29 | 王晓雯 | A kind of fulvic acid microbial bacterial agent and preparation method thereof |
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Application publication date: 20140205 |