CN103548853A - Application of stephanine in preparation of plant source molluscacide - Google Patents
Application of stephanine in preparation of plant source molluscacide Download PDFInfo
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- CN103548853A CN103548853A CN201310571603.2A CN201310571603A CN103548853A CN 103548853 A CN103548853 A CN 103548853A CN 201310571603 A CN201310571603 A CN 201310571603A CN 103548853 A CN103548853 A CN 103548853A
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- stephanine
- molluscacide
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- oncomelania
- plant resource
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Abstract
The invention discloses an application of stephanine in preparation of a plant source molluscacide. Thus, the application of the molluscacide is expanded; a new path is supplied for material selection of the plant source molluscacide.
Description
Technical field
What the present invention relates to is a kind of stephanine, the in particular application of a kind of stephanine in preparing plant resource molluscacide.
Background technology
Schistosomiasis is one of WTO regulation " six large tropical diseases ", one of China's " five large parasitosis ", and not only serious harm health of schistosomiasis also can cause great harm to domestic animal simultaneously.Oncomelania is the unique vector of Schistosoma japonicum, and willful murder water-snail eradication is the important bad joint of controlling schistosomiasis propagation.But at present the various spiral shell methods of killing all have deficiency, as best in the go out effect of spiral shell of chemicals, but environmental pollution is serious.Niclosamidum is the chemical snail killing agent of current unique recommendation, because it is to shortcomings such as fish toxicity are large, expensive, in practical application, is restricted.At present, many experts are just being devoted to efficiently, exploitation and the research of low toxicity, inexpensive molluscicide, wherein plant extracts molluscicide is as an a kind of class material of pure natural, there is effect stability, have no side effect, noresidue, the advantage such as environmentally friendly, therefore from plant, screening molluscicide becomes people's study hotspot in recent years.
Japanese Stephania Root is root or the cauline leaf of menispermaceous plants Japanese Stephania Root, be a kind of important traditional Chinese medicine, have another name called gold thread and angle tortoise, Fourstamen Stephania Root, public mouse rattan, wild peach grass, firecracker to disappear etc., have clearing heat and detoxicating, inducing diuresis to reduce edema, the effects such as dispelling, collateral-activating, are widely used in preventing and curing malaria disease, dysentery, arthralgia due to wind-dampness, oedema, in the diseases such as abscess of throat, but its application in Killing Oncomelania Hupensis, prevention schistosomiasis have not been reported.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, the application of a kind of stephanine in preparing plant resource molluscacide is provided.
The present invention relates to the application of a kind of stephanine in preparing plant resource molluscacide, through test, find, stephanine is Killing Oncomelania Hupensis effectively, prevention and cure of schistosomiasis, simultaneously, the efficiency of stephanine Killing Oncomelania Hupensis increases along with the increase of concentration, there is obvious dose effect, when the concentration of stephanine is when 12.5mg/L is above, the efficiency of its Killing Oncomelania Hupensis is 100%, described plant resource molluscacide is that stephanine water directly configures and forms, can only contain stephanine as unique active component, also can contain other active component except Japanese Stephania Root, as other has the composition of activity against snails.
Preferably, described stephanine has structure as follows:
Preferably, the concentration of described stephanine Killing Oncomelania Hupensis is more than 6.25mg/L, and the time of Killing Oncomelania Hupensis is more than 24 hours.
Preferably, the concentration of described stephanine Killing Oncomelania Hupensis is more than 12.5mg/L, and the time of Killing Oncomelania Hupensis is more than 48 hours.
The present invention has the following advantages compared to existing technology: the application of a kind of stephanine provided by the invention in preparing plant resource molluscacide, widened the range of application of stephanine, and also the selection for plant resource molluscacide provides a kind of new approach.
Embodiment
Below embodiments of the invention are elaborated, the present embodiment is implemented take technical solution of the present invention under prerequisite, provided detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
Embodiment 1
The present embodiment provides a kind of preparation method of stephanine, comprises the following steps:
(1) get Japanese Stephania Root medicinal material, be crushed to 40 orders, adopt supercritical CO
2extraction, collects extract;
(2) the dissolve with hydrochloric acid solution extract that is 1% by percent by volume, filters, and adds in cationic exchange resin adsorption post and adsorbs, and then water flushing adsorption column to efflux is neutral, finally with alkaline alcohol, carries out wash-out, obtains eluent;
(3) eluent is carried out to reduced pressure concentration, obtain concentrate, add aluminium oxide in concentrate, mix after oven dry, adding volume fraction is that 50% methanol solution carries out ultrasonic extraction, obtains extract;
(4) extract is carried out to reduced pressure concentration, with ammoniacal liquor, regulate pH value to pH=10, standing crystallization, leaches crystal, then with ethanolic solution recrystallization, obtains stephanine crystal, finally that crystal is dry, obtains described stephanine.
Embodiment 2
The preparation method of the another kind of stephanine that the present embodiment provides, comprises the following steps:
(1) get Japanese Stephania Root medicinal material, be crushed to 80 orders, adopt supercritical CO
2extraction, collects extract;
(2) the sulfuric acid solution dissolution extraction thing that is 2% by percent by volume, filters, and adds in cationic exchange resin adsorption post and adsorbs, and then water flushing adsorption column to efflux is neutral, finally with alkaline alcohol, carries out wash-out, obtains eluent;
(3) eluent is carried out to reduced pressure concentration, obtain concentrate, add aluminium oxide in concentrate, mix after oven dry, adding volume fraction is that 50% methanol solution carries out ultrasonic extraction, obtains extract;
(4) extract is carried out to reduced pressure concentration, with ammoniacal liquor, regulate pH value to pH=12, standing crystallization, leaches crystal, then with ethanolic solution recrystallization, obtains stephanine crystal, finally that crystal is dry, obtains described stephanine.
Embodiment 3
The present embodiment is stephanine as the test of pesticide effectiveness of plant resource molluscacide:
1, experimental condition: 26 ± 1 ℃ of temperature, relative moisture 65 ± 5%.
2, for examination oncomelania: the oncomelania hupensis of Anhui Province's collection in worksite, screens 6~8 feminine genders of revolving and becomes spiral shell as supplying examination oncomelania.
3, the concentration of stephanine: get the stephanine that embodiment 1 prepares and be diluted to 66.6mg/L with dechlorination water, add tween that stephanine is dissolved completely in water, be the plant resource molluscacide for examination.
4, soak and kill test: by 20, for examination oncomelania, put into beaker, get the above-mentioned plant resource molluscacide submergence configuring for examination oncomelania, with plastic window screening, be placed on beaker mouth in case oncomelania climbs out of, establish negative control simultaneously, soak and kill after one day, remove molluscacide, recover to raise 48 hours, with hammering method, identify the death toll for examination oncomelania.
Separately get 20, with above-mentioned soaking, kill test and soak and kill 48 hours, renewal cultivation 48 hours, calculates death toll.
5, result of the test:
Result is as shown in table 1 below:
Table 1: stephanine solution soaks the result statistical form that kills oncomelania test
Process | Dosage (mg/L) | 24h death toll | 24h lethality (%) | 48h death toll | 48h lethality (%) |
Stephanine | 66.6 | 5 | 25 | 20 | 100 |
Contrast | 0 | 0 | 0 | 0 | 0 |
Embodiment 4
The present embodiment is variable concentrations stephanine as the test of pesticide effectiveness of plant resource molluscacide:
In the present embodiment, the quantity of test oncomelania is 30, and the concentration of stephanine is 200mg/L, 100mg/L, 50mg/L, 25mg/L, 12.5mg/L, 0mg/L(negative control), soaking kills the time is 48 hours, other step is with embodiment 3, and result of the test is as shown in table 2 below:
Table 2: variable concentrations stephanine solution soaks the result statistical form that kills oncomelania test
Embodiment 5
The present embodiment is variable concentrations stephanine as the test of pesticide effectiveness of plant resource molluscacide:
In the present embodiment, the quantity of test oncomelania is 30, and the concentration of stephanine is 50mg/L, 25mg/L, 12.5mg/L, 6.25mg/L, 3.1mg/L, 1.5mg/L, 0mg/L(negative control), soaking kills the time is 48 hours, other step is with embodiment 3, and result of the test is as shown in table 3 below:
Table 3: variable concentrations stephanine solution soaks the result statistical form that kills oncomelania test
The test of pesticide effectiveness result of embodiment 3~5 shows, the concentration of stephanine is when 12.5mg/L is above, the efficiency of its Killing Oncomelania Hupensis is 100%, hence one can see that, stephanine can kill oncomelania effectively as plant resource molluscacide, reach the object of prevention and cure of schistosomiasis, simultaneously, from result of the test, it can also be seen that, with stephanine, as the medial lethal dose LD50 of plant resource molluscacide, be 6.25mg/L, medial lethal dose LD50 determines, for stephanine provides guidance foundation as the safe medication of plant resource molluscacide.
Claims (4)
1. the stephanine application in preparing plant resource molluscacide.
3. the application of a kind of stephanine as claimed in claim 1 in preparing plant resource molluscacide, is characterized in that, the concentration of described stephanine Killing Oncomelania Hupensis is more than 6.25mg/L, and the time of Killing Oncomelania Hupensis is more than 24 hours.
4. the application of a kind of stephanine as claimed in claim 3 in preparing plant resource molluscacide, is characterized in that, the concentration of described stephanine Killing Oncomelania Hupensis is more than 12.5mg/L, and the time of Killing Oncomelania Hupensis is more than 48 hours.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107736362A (en) * | 2017-11-03 | 2018-02-27 | 安徽农业大学 | A kind of method that sodium alginate investment prepares flora-originated molluscicide |
CN110590463A (en) * | 2019-10-25 | 2019-12-20 | 湖北大学 | Fertilizer and molluscicide double-effect organic fertilizer molluscicide, preparation method and application thereof |
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JP2011105602A (en) * | 2009-11-12 | 2011-06-02 | Ohta Oil Mill Co Ltd | Repellent against mollusk belonging to gastropod and manufacturing method of the same |
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CN101518250A (en) * | 2009-04-10 | 2009-09-02 | 湖北大学 | Camptothecin plant molluscacide and preparation and application method thereof |
JP2011105602A (en) * | 2009-11-12 | 2011-06-02 | Ohta Oil Mill Co Ltd | Repellent against mollusk belonging to gastropod and manufacturing method of the same |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107736362A (en) * | 2017-11-03 | 2018-02-27 | 安徽农业大学 | A kind of method that sodium alginate investment prepares flora-originated molluscicide |
CN107736362B (en) * | 2017-11-03 | 2020-11-06 | 安徽农业大学 | Method for preparing botanical molluscicide by sodium alginate embedding method |
CN110590463A (en) * | 2019-10-25 | 2019-12-20 | 湖北大学 | Fertilizer and molluscicide double-effect organic fertilizer molluscicide, preparation method and application thereof |
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