CN103548853B - Application of stephanine in preparation of plant source molluscacide - Google Patents

Application of stephanine in preparation of plant source molluscacide Download PDF

Info

Publication number
CN103548853B
CN103548853B CN201310571603.2A CN201310571603A CN103548853B CN 103548853 B CN103548853 B CN 103548853B CN 201310571603 A CN201310571603 A CN 201310571603A CN 103548853 B CN103548853 B CN 103548853B
Authority
CN
China
Prior art keywords
stephanine
molluscacide
application
oncomelania
test
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201310571603.2A
Other languages
Chinese (zh)
Other versions
CN103548853A (en
Inventor
方从兵
宁井铭
王智慧
方访
汪小钢
孙启祥
彭镇华
韦朝领
黄成林
张倩
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Anhui Agricultural University AHAU
Original Assignee
Anhui Agricultural University AHAU
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Anhui Agricultural University AHAU filed Critical Anhui Agricultural University AHAU
Priority to CN201310571603.2A priority Critical patent/CN103548853B/en
Publication of CN103548853A publication Critical patent/CN103548853A/en
Application granted granted Critical
Publication of CN103548853B publication Critical patent/CN103548853B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Abstract

The invention discloses an application of stephanine in preparation of a plant source molluscacide. Thus, the application of the molluscacide is expanded; a new path is supplied for material selection of the plant source molluscacide.

Description

A kind of stephanine is preparing the application in plant resource molluscacide
Technical field
What the present invention relates to is a kind of stephanine, and in particular a kind of stephanine is preparing the application in plant resource molluscacide.
Background technology
Schistosomiasis is one of WTO regulation " six large tropical diseases ", and one of China's " five large parasitosis ", schistosomiasis not only serious harm health, also can cause great harm to domestic animal simultaneously.Oncomelania is the unique vector of Schistosoma japonicum, and willful murder water-snail eradication is the important bad joint that control schistosomiasis is propagated.But various spiral shell method of killing all has deficiency at present, as best in the go out effect of spiral shell of chemicals, but environmental pollution is serious.Niclosamidum is current unique chemical snail killing agent recommended, and because of its shortcoming such as large to fish toxicity, expensive, is restricted in practical application.At present, many experts are just being devoted to efficiently, the exploitation of low toxicity, inexpensive molluscicide and research, wherein plant extracts molluscicide is as an a kind of class material of pure natural, there is effect stability, have no side effect, noresidue, the advantage such as environmentally friendly, from plant, therefore screen the study hotspot that molluscicide becomes people in recent years.
Japanese Stephania Root is root or the cauline leaf of menispermaceous plants Japanese Stephania Root, be a kind of important traditional Chinese medicine, have another name called gold thread and fish tortoise, Fourstamen Stephania Root, public mouse rattan, wild peach grass, firecracker to disappear, have clearing heat and detoxicating, inducing diuresis to reduce edema, the effects such as dispelling, collateral-activating, are widely used in preventing and curing malaria disease, dysentery, arthralgia due to wind-dampness, oedema, in the diseases such as abscess of throat, but its Killing Oncomelania Hupensis, prevention schistosomiasis on application have not been reported.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, provide a kind of stephanine and preparing the application in plant resource molluscacide.
The present invention relates to a kind of stephanine and prepare the application in plant resource molluscacide, find through test, stephanine can effective Killing Oncomelania Hupensis, prevention and cure of schistosomiasis, simultaneously, the efficiency of stephanine Killing Oncomelania Hupensis increases along with the increase of concentration, there is obvious dose effect, when Japanese Stephania Root paper mill wastewater is at more than 12.5mg/L, the efficiency of its Killing Oncomelania Hupensis is 100%, described plant resource molluscacide is that stephanine water directly configures and forms, can only contain stephanine as sole active agent, also can containing other active component except Japanese Stephania Root, as other has the composition of activity against snails.
Preferably, described stephanine has structure as follows:
Preferably, the concentration of described stephanine Killing Oncomelania Hupensis is more than 6.25mg/L, and the time of Killing Oncomelania Hupensis is more than 24 hours.
Preferably, the concentration of described stephanine Killing Oncomelania Hupensis is more than 12.5mg/L, and the time of Killing Oncomelania Hupensis is more than 48 hours.
The present invention has the following advantages compared to existing technology: a kind of stephanine provided by the invention is preparing the application in plant resource molluscacide, has widened the range of application of stephanine, also for the selection of plant resource molluscacide provides a kind of new approach.
Embodiment
Elaborate to embodiments of the invention below, the present embodiment is implemented under premised on technical solution of the present invention, give detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
Embodiment 1
The present embodiment provides a kind of preparation method of stephanine, comprises the following steps:
(1) get Japanese Stephania Root medicinal material, be crushed to 40 orders, adopt supercritical CO 2extraction, collects extract;
(2) by percent by volume be 1% dissolve with hydrochloric acid solution extract, filter, add in cationic exchange resin adsorption post and adsorb, then rinse adsorption column to efflux in neutral with water, finally carry out wash-out with alkaline alcohol, obtain eluent;
(3) eluent is carried out reduced pressure concentration, obtain concentrate, in concentrate, add aluminium oxide, mixing dry after, add volume fraction be 50% methanol solution carry out ultrasonic extraction, obtain extract;
(4) extract is carried out reduced pressure concentration, by ammoniacal liquor adjust ph to pH=10, leave standstill crystallization, leach crystal, then with ethanolic solution recrystallization, obtain stephanine crystal, finally that crystal is dry, obtain described stephanine.
Embodiment 2
The preparation method of the another kind of stephanine that the present embodiment provides, comprises the following steps:
(1) get Japanese Stephania Root medicinal material, be crushed to 80 orders, adopt supercritical CO 2extraction, collects extract;
(2) by percent by volume be 2% sulfuric acid solution dissolution extraction thing, filter, add in cationic exchange resin adsorption post and adsorb, then rinse adsorption column to efflux in neutral with water, finally carry out wash-out with alkaline alcohol, obtain eluent;
(3) eluent is carried out reduced pressure concentration, obtain concentrate, in concentrate, add aluminium oxide, mixing dry after, add volume fraction be 50% methanol solution carry out ultrasonic extraction, obtain extract;
(4) extract is carried out reduced pressure concentration, by ammoniacal liquor adjust ph to pH=12, leave standstill crystallization, leach crystal, then with ethanolic solution recrystallization, obtain stephanine crystal, finally that crystal is dry, obtain described stephanine.
Embodiment 3
The present embodiment is the test of pesticide effectiveness of stephanine as plant resource molluscacide:
1, experimental condition: temperature 26 ± 1 DEG C, relative moisture 65 ± 5%.
2, for examination oncomelania: the oncomelania hupensis of Anhui Province's collection in worksite, the feminine gender that screening 6 ~ 8 is revolved becomes spiral shell as supplying examination oncomelania.
3, Japanese Stephania Root paper mill wastewater: the stephanine dechlorination water that Example 1 prepares is diluted to 66.6mg/L, adds tween and stephanine is dissolved completely in water, is the plant resource molluscacide for examination.
4, test is killed in leaching: put into beaker by 20 for examination oncomelania, get the above-mentioned plant resource molluscacide submergence configured for examination oncomelania, beaker mouth is placed in case oncomelania climbs out of with plastic window screening, establish negative control simultaneously, after leaching kills one day, remove molluscacide, recover raising 48 hours, by the death toll of hammering method qualification for examination oncomelania.
Separately get 20, kill test leaching with above-mentioned leaching and kill 48 hours, renewal cultivation 48 hours, calculate death toll.
5, result of the test:
Result is as shown in table 1 below:
Table 1: the result statistical form of oncomelania test is killed in the leaching of stephanine solution
Process Dosage (mg/L) 24h death toll 24h lethality (%) 48h death toll 48h lethality (%)
Stephanine 66.6 5 25 20 100
Contrast 0 0 0 0 0
Embodiment 4
The present embodiment is the test of pesticide effectiveness of variable concentrations stephanine as plant resource molluscacide:
In the present embodiment, the quantity of test oncomelania is 30, and Japanese Stephania Root paper mill wastewater is 200mg/L, 100mg/L, 50mg/L, 25mg/L, 12.5mg/L, 0mg/L(negative control), it is 48 hours that leaching is killd the time, other step is with embodiment 3, and result of the test is as shown in table 2 below:
Table 2: the result statistical form of oncomelania test is killed in the leaching of variable concentrations stephanine solution
Embodiment 5
The present embodiment is the test of pesticide effectiveness of variable concentrations stephanine as plant resource molluscacide:
In the present embodiment, the quantity of test oncomelania is 30, and Japanese Stephania Root paper mill wastewater is 50mg/L, 25mg/L, 12.5mg/L, 6.25mg/L, 3.1mg/L, 1.5mg/L, 0mg/L(negative control), it is 48 hours that leaching is killd the time, other step is with embodiment 3, and result of the test is as shown in table 3 below:
Table 3: the result statistical form of oncomelania test is killed in the leaching of variable concentrations stephanine solution
The test of pesticide effectiveness result of embodiment 3 ~ 5 shows, Japanese Stephania Root paper mill wastewater is when more than 12.5mg/L, the efficiency of its Killing Oncomelania Hupensis is 100%, it can thus be appreciated that, stephanine can kill oncomelania effectively as plant resource molluscacide, reach the object of prevention and cure of schistosomiasis, simultaneously, it can also be seen that from result of the test, be 6.25mg/L with stephanine as the medial lethal dose LD50 of plant resource molluscacide, the determination of medial lethal dose LD50, for stephanine provides guidance foundation as the safe medication of plant resource molluscacide.

Claims (3)

1. stephanine is preparing the application in plant resource molluscacide, and described stephanine has structure as follows:
2. a kind of stephanine as claimed in claim 1 is preparing the application in plant resource molluscacide, it is characterized in that, the concentration of described stephanine Killing Oncomelania Hupensis is more than 6.25mg/L, and the time of Killing Oncomelania Hupensis is more than 24 hours.
3. a kind of stephanine as claimed in claim 1 is preparing the application in plant resource molluscacide, it is characterized in that, the concentration of described stephanine Killing Oncomelania Hupensis is more than 12.5mg/L, and the time of Killing Oncomelania Hupensis is more than 48 hours.
CN201310571603.2A 2013-11-13 2013-11-13 Application of stephanine in preparation of plant source molluscacide Expired - Fee Related CN103548853B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201310571603.2A CN103548853B (en) 2013-11-13 2013-11-13 Application of stephanine in preparation of plant source molluscacide

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201310571603.2A CN103548853B (en) 2013-11-13 2013-11-13 Application of stephanine in preparation of plant source molluscacide

Publications (2)

Publication Number Publication Date
CN103548853A CN103548853A (en) 2014-02-05
CN103548853B true CN103548853B (en) 2015-06-03

Family

ID=50003362

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201310571603.2A Expired - Fee Related CN103548853B (en) 2013-11-13 2013-11-13 Application of stephanine in preparation of plant source molluscacide

Country Status (1)

Country Link
CN (1) CN103548853B (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107736362B (en) * 2017-11-03 2020-11-06 安徽农业大学 Method for preparing botanical molluscicide by sodium alginate embedding method
CN110590463B (en) * 2019-10-25 2021-10-15 湖北大学 Fertilizer and molluscicide double-effect organic fertilizer molluscicide, preparation method and application thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101518250A (en) * 2009-04-10 2009-09-02 湖北大学 Camptothecin plant molluscacide and preparation and application method thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011105602A (en) * 2009-11-12 2011-06-02 Ohta Oil Mill Co Ltd Repellent against mollusk belonging to gastropod and manufacturing method of the same

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101518250A (en) * 2009-04-10 2009-09-02 湖北大学 Camptothecin plant molluscacide and preparation and application method thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
92种野生和栽培植物或中草药的提取物杀灭钉螺筛选试验;冯新港 等;《中国血吸虫病防治杂志》;20021231;第14卷(第6期);第412-417页 *
季宇彬.千金藤碱.《中药有效成分药理与应用》.黑龙江科学技术出版社,1995,第442页. *
汪鋆植 等.千金藤碱.《抗病毒中药学》.化学工业出版社,2007,第128页. *

Also Published As

Publication number Publication date
CN103548853A (en) 2014-02-05

Similar Documents

Publication Publication Date Title
CN104098405B (en) Micro-fertilizer of Chelating state having desinsection concurrently and preparation method thereof
CN103548853B (en) Application of stephanine in preparation of plant source molluscacide
CN103356789A (en) Zanthoxylum armatum total alkaloids extract, and preparation method and application thereof
CN103461397B (en) A kind of natural pesticide for gingko and preparation method thereof
CN104855382A (en) Application of Pedunsaponin A and Pedunsaponin C in preparation of molluscacide
CN105248489A (en) Biological agent for preventing pests and diseases in tea garden and preparation method thereof
CN105746592A (en) Biogenic herbicide and preparation method thereof
Rahman et al. Analgesic and anti-inflammatory effects of Crinum asiaticum leaf alcoholic extract in animal models
CN104396957A (en) Microemulsion for controlling tea-tree tea geometrid
CN102524314B (en) Euphorbia esula plant pest-killing emulsion in water
CN100431424C (en) Preparation method and use of a fish toxic made of tea saponin
CN1830263A (en) Biological pesticide composition and its preparation method and use
CN103948583B (en) Morindone G and/or morindone O is preparing the application killed in eliminating fish parasites medicine
CN103877155A (en) Chinese herbal preparation for treating trichodiniasis of fishes
CN101422178B (en) Berberine imidacloprid water agent
CN103948616B (en) Upright Radix Cynanchi Atrati glycosides C kills the application of the ectoparasite ciliate medicine of Fish in preparation
CN207235918U (en) A kind of device being administered at regular time and quantity
CN105076234B (en) A kind of its preparation technology of the plant insecticide of preventing and treating sitophilus zea-mais
CN105230623A (en) Application of scoparone as acaricide
CN105016475A (en) Synthetic method for biological preparation capable of enriching content of heavy metal mercury in paddy field
CN101088339B (en) Composite molluscacide containing ginkgolic acid and its preparation process
CN106577844B (en) Prevent and treat capsicum epidemic disease preparation, its preparation and application
CN1788572A (en) Method for preparing aqueous formulation for controlling melon powdery mildew using prostrate spurge extract
CN100356854C (en) Plant agent for killing snails, prepn. method and use method thereof
CN104206392A (en) Complex resistance inducer for prevention of tobacco plant virus and fungal disease, and preparation method and application thereof

Legal Events

Date Code Title Description
PB01 Publication
C06 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
C14 Grant of patent or utility model
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20150603

Termination date: 20151113

EXPY Termination of patent right or utility model