CN103548840B - Sterilization compound containing benzothiostrobin and difenoconazole and application thereof - Google Patents
Sterilization compound containing benzothiostrobin and difenoconazole and application thereof Download PDFInfo
- Publication number
- CN103548840B CN103548840B CN201310520873.0A CN201310520873A CN103548840B CN 103548840 B CN103548840 B CN 103548840B CN 201310520873 A CN201310520873 A CN 201310520873A CN 103548840 B CN103548840 B CN 103548840B
- Authority
- CN
- China
- Prior art keywords
- difenoconazole
- bacterium ester
- benzene thiophene
- thiophene bacterium
- bactericidal composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention relates to a sterilization compound containing benzothiostrobin and difenoconazole. The sterilization compound comprises active ingredients and pesticide preparation auxiliary components, wherein the active ingredients comprise benzothiostrobin and difenoconazole. The benzothiostrobin and the difenoconazole in the sterilization compound provided by the invention belong to two bactericides with different mechanisms, and can be compounded within a certain proportion range to generate a remarkable synergistic role. The sterilization compound is remarkable in prevention and control effect, is safe to crops without damage risk, is reduced in acute toxicity to mammals, and is improved in safety.
Description
Technical field
The invention belongs to pesticides application technical field, relate to a kind of bactericidal composition that contains benzene thiophene bacterium ester and Difenoconazole, also relate to the application of bactericidal composition.
Background technology
Benzene thiophene bacterium ester belongs to methoxy acrylic bactericide, and the mechanism of action is to suppress mitochondrial respiratory effect, is the environmentally friendly bactericide of a kind of efficient, wide spectrum, low toxicity.In addition benzene thiophene bacterium ester, owing to protecting preferably, treat, rooting out bactericidal effect, has good control efficiency to powdery mildew, yellow rust, downy mildew, leaf spot and rice blast on fruit tree, vegetables and cereal crop.
Difenoconazole belongs to triazole bactericidal agent, synthetic by Antifungi ergosterol, the formation of block cell wall, is the C-14 demethylation enzyme inhibitor in sterol biosynthesis, under recommended dose to crop safety, without poisoning, to underground water and Environmental security.Difenoconazole can be used for cauline leaf spraying and seed treatment on field crop, fruit tree, vegetables and tea tree.It has efficiently, wide spectrum, protection, treat, root out, the multiple effect of interior suction, effectively control the fungal diseases such as scab, anthrachose of grape, white rot, spot defoliation, powdery mildew, brown spot, yellow rust, bar yellow rust, head blight.
In agricultural production, fungal disease is damage to crops growth principal element, and kind is many, host range is wide, harm is serious.Control at present crop fungal disease mainly by chemical control.Long-term chemical control inevitable outcome is that induction pathogen develops immunity to drugs.Drug-fast generation causes medicament to decline to disease-controlling effect, causes the agriculture underproduction.Report that at present the diseases such as wheat powdery mildew, cucumber downy mildew, fruit tree yellow rust have produced pesticide resistance in various degree to many bactericide.
In prior art, two or more agricultural chemicals single dose is used in combination according to a certain percentage to a kind of important way that becomes pesticides application.Good compounded formula not only can expand pesticide control object and range of application, improves drug effect, reduces toxicity, poisoning and residual, can also overcome and slow down pesticide resistance and produce.Yet be not that any two kinds of agricultural chemicals single doses can composite like this use, because of this composite problem that also exists, as although some active component is used problem little separately, but after composite use, may there is bad chemical reaction and cause problem of environmental pollution in the intermediate product producing in their degradation process.Benzene thiophene bacterium ester and Difenoconazole compounded combination there is no report at present.
Summary of the invention
Technical problem to be solved by this invention is to overcome the deficiencies in the prior art, and the bactericidal composition that a kind of control efficiency is good, resistance is low, safety is good is provided.
For solving above technical problem, the present invention adopts following technical scheme:
A bactericidal composition that contains benzene thiophene bacterium ester and Difenoconazole, it is comprised of active component and pesticidal preparations auxiliary element, and described active component is comprised of benzene thiophene bacterium ester and Difenoconazole.
In above-mentioned active component, the mass ratio of benzene thiophene bacterium ester and Difenoconazole is 1:8 to 8:1.
Further, in described active component, the mass ratio of benzene thiophene bacterium ester and Difenoconazole is 1:6 to 6:1
Further, in described active component, the mass ratio of benzene thiophene bacterium ester and Difenoconazole is 1:4 to 4:1.
Further, in described active component, the mass ratio of benzene thiophene bacterium ester and Difenoconazole is 1:2 to 2:1.
Further, in described active component, the mass ratio of benzene thiophene bacterium ester and Difenoconazole is 3:5.
According to the present invention, bactericidal composition can be made different formulations when practical application, comprises missible oil, wetting powder, suspending agent, water dispersible granules.
Further, in above-mentioned formulation, the mass content of the two gross mass of benzene thiophene bacterium ester and Difenoconazole in described bactericidal composition is 10%～80%.
Bactericidal composition of the present invention is remarkable to the control efficiency of the Common Diseases of fruit tree, vegetables and cereal crop, and is of value to and delays the drug-fast generation of germ.Described disease is plant epiphyte venereal disease evil.
For this reason, the present invention is also particularly related to the application of the above-mentioned bactericidal composition that contains benzene thiophene bacterium ester and Difenoconazole in powdery mildew, yellow rust and the anthracnose of control fruit tree, vegetables and cereal crop.
Due to the enforcement of technique scheme, the present invention compared with prior art tool has the following advantages:
The bactericide that composition benzene thiophene bacterium ester in bactericidal composition of the present invention and Difenoconazole belong to two kinds of different mechanism, both are carried out within the scope of certain proportion to composite energy and produce obvious synergistic function, composition for preventing and controlling effect is remarkable, to Crop securify without poisoning, and composite rear to the reduction of mammal acute toxicity, improved safety.
Bactericidal composition of the present invention and benzene thiophene bacterium ester, Difenoconazole are used separately comparison, and expanding prevention spectrum delays pesticide resistance and produces, and dosage is little, and drug cost is low, better cover crop safety and ecotope.
Embodiment
The toxicity test of bactericidal composition of the present invention to plant powdery mildew, yellow rust, anthracnose
1. the toxicity test of bactericidal composition of the present invention to plant powdery mildew
Test material is powdery mildew of cucumber leaf.With the pure water that is added with a small amount of surfactant (as Tween-80), wash and get the fresh spore covering with on Powdery Mildew cucumber leaves, with double gauze, filter, the spore concentration of making is 1 * 10
5the suspension of individual spore/mL, standby.
Test adopts spore suspension spray inoculation.After the true mounted blade of potted plant cucumber seedling growth to 4, choose healthy and strong healthy plant for connecing bacterium test, every basin is planted 5 strains, and every processing 3 basins repeat for 4 times.Every basin spray bacterium liquid 5mL, inoculation 24h spraying liquid.Liquid is mixed with needed preparation by applicant by former medicine, on pretreatment basis, series concentration is set, and establishes and only have solvent and surfactant and containing the processing of active ingredient, do not make blank.After inoculation, examination material natural air drying, then moves to thermostatic chamber, under the condition of 20 ℃～24 ℃ of temperature, cultivates 10d.Until the blank incidence of disease, reach 80% when above, incidence is managed in classification investigation everywhere, and 30 leaves are at least investigated in every processing, grade scale:
0 grade: anosis;
1 grade: lesion area accounts for below 5% of one-piece blade area;
3 grades: lesion area accounts for the 6%-10% of one-piece blade area;
5 grades: lesion area accounts for the 11%-25% of one-piece blade area;
7 grades: lesion area accounts for the 26%-50% of one-piece blade area;
9 grades: lesion area accounts for the more than 50% of one-piece blade area;
Data analysing method:
Control efficiency is scaled to probability value, drug concentration is converted to logarithm value, with DPS data handling system software, logarithm value and probability value are carried out to regression analysis, calculate the EC50 of each medicament, according to the abundant method of Sun Yun, calculate the mixed co-toxicity coefficient (CTC value) of medicament toxicity index level, CTC value is greater than 100 for synergistic effect, is less than 100 for antagonism.
2. the toxicity test of bactericidal composition of the present invention to plant yellow rust
Test material is stripe rust of wheat cereal cultivars.20 seeds of every basin sowing, after emerging, selected wherein 10 strains, standby while growing to 2 leaf 1 core.The fresh uredospore of the rest fungus producing in 24h on incidence of leaf, with being added with under the pure washing of a small amount of surfactant (as Tween-80), is filtered with double gauze, and the spore concentration of making is 1 * 10
5the suspension of individual spore/mL, standby.
Test adopts spore suspension spray inoculation.Choose the plant of robust growth for connecing bacterium test, every processing 1 basin, repeats for 4 times.Every basin spray bacterium liquid 5mL, spraying liquid after inoculation 24h.Liquid is mixed with needed preparation by applicant by former medicine, on pretreatment basis, series concentration is set, and establishes and only have solvent and surfactant and containing the processing of active ingredient, do not make blank.More than after inoculation, 12h is cultivated in dark moisturizing, the optimum temperature that the moisturizing stage requires is 15 ℃～20 ℃, then moves to thermostatic chamber, cultivates, more than illumination every day 12h under the condition of 18 ℃～24 ℃ of temperature.Until the blank incidence of disease, reach 80% when above, incidence, grade scale are managed in classification investigation everywhere:
0 grade: without sorus;
1 grade: sorus accounts for below 5% of one-piece blade area;
3 grades: sorus accounts for the 6%-10% of one-piece blade area;
5 grades: sorus accounts for the 11%-25% of one-piece blade area;
7 grades: sorus accounts for the 26%-50% of one-piece blade area;
9 grades: sorus accounts for the more than 50% of one-piece blade area;
Data analysing method:
Control efficiency is scaled to probability value, and drug concentration is converted to logarithm value, with DPS data handling system software, logarithm value and probability value is carried out to regression analysis, calculates the EC of each medicament
50, according to the abundant method of Sun Yun, calculate the mixed co-toxicity coefficient (CTC value) of medicament toxicity index level, CTC value is greater than 100 for synergistic effect, is less than 100 for antagonism.
3. the toxicity test of bactericidal composition of the present invention to plant anthracnose
Test material is cucumber anthracnose kind.Be cultured to 2～4 leaf periods standby.Test is cultivated on suitable medium with pathogen, after spore to be generated, with spore under aseptic washing, and filter with double gauze, the spore concentration of making is 1 * 10
5the suspension of individual spore/mL, standby.
Test adopts microscale sampler to draw 10 μ L spore suspensions and is inoculated in face of blade, and every processing is no less than 40 vaccinations.Every basin 5 strains, every processing 2 basins, repeat for 4 times.Spraying liquid after inoculation 24h, liquid is mixed with needed preparation by applicant by former medicine, on pretreatment basis, series concentration is set, liquid medicine jet is imposed on to blade to all wetting, standby after liquid natural air drying, and establish and only have solvent and surfactant and containing the processing of active ingredient, do not make blank.After inoculation, move in humidistat more than (relative moisture 95%～100%) dark moisturizing cultivates 22h, then move to thermostatic chamber, under the condition of 22 ℃～27 ℃ of temperature, intensity of illumination 20000Lux, humidity 80%～90%, cultivate.Until the blank incidence of disease, reach 50% when above, with slide measure with right-angled intersection normal beam technique measure scab diameter each once, average, unit millimeter (mm) meter.
Control efficiency is scaled to probability value, and drug concentration is converted to logarithm value, with DPS data handling system software, logarithm value and probability value is carried out to regression analysis, calculates the EC of each medicament
50, according to the abundant method of Sun Yun, calculate the mixed co-toxicity coefficient (CTC value) of medicament toxicity index level, CTC value is greater than 100 for synergistic effect, is less than 100 for antagonism.
For the measurement result of powdery mildew of cucumber, stripe rust of wheat, cucumber anthracnose respectively in Table 1, table 2 and table 3:
The difference composite toxicity test result to powdery mildew of cucumber of table 1 benzene thiophene bacterium ester and Difenoconazole
| Process | EC 50（mg/L） | Co-toxicity coefficient (CTC) |
| Benzene thiophene bacterium ester 1+ Difenoconazole 8 | 6.02 | 162.38 |
| Benzene thiophene bacterium ester 1+ Difenoconazole 6 | 5.43 | 177.65 |
| Benzene thiophene bacterium ester 3+ Difenoconazole 5 | 2.15 | 279.54 |
| Benzene thiophene bacterium ester 5+ Difenoconazole 3 | 3.02 | 216.51 |
| Benzene thiophene bacterium ester 6+ Difenoconazole 1 | 5.11 | 188.77 |
| Benzene thiophene bacterium ester 8+ Difenoconazole 1 | 4.32 | 170.82 |
| Benzene thiophene bacterium ester | 7.13 | - |
| Phenylate collarium azoles | 10.25 | - |
Table 2 benzene thiophene bacterium ester and Difenoconazole difference composite to stripe rust of wheat toxicity test result
| Process | EC 50（mg/L） | Co-toxicity coefficient (CTC) |
| Benzene thiophene bacterium ester 1+ Difenoconazole 8 | 7.02 | 214.86 |
| Benzene thiophene bacterium ester 1+ Difenoconazole 6 | 6.63 | 225.67 |
| Benzene thiophene bacterium ester 3+ Difenoconazole 5 | 4.35 | 325.00 |
| Benzene thiophene bacterium ester 5+ Difenoconazole 3 | 4.82 | 276.86 |
| Benzene thiophene bacterium ester 6+ Difenoconazole 1 | 6.14 | 243.68 |
| Benzene thiophene bacterium ester 8+ Difenoconazole 1 | 7.32 | 172.12 |
| Benzene thiophene bacterium ester | 12.31 | - |
| Phenylate collarium azoles | 15.52 | - |
Table 3 benzene thiophene bacterium ester and Difenoconazole difference composite to cucumber anthracnose toxicity test result
| Process | EC 50（mg/L） | Co-toxicity coefficient (CTC) |
| Benzene thiophene bacterium ester 1+ Difenoconazole 8 | 3.22 | 173.21 |
| Benzene thiophene bacterium ester 1+ Difenoconazole 6 | 2.53 | 225.84 |
| Benzene thiophene bacterium ester 3+ Difenoconazole 5 | 1.35 | 272.63 |
| Benzene thiophene bacterium ester 5+ Difenoconazole 3 | 3.02 | 210.32 |
| Benzene thiophene bacterium ester 6+ Difenoconazole 1 | 3.44 | 180.05 |
| Benzene thiophene bacterium ester 8+ Difenoconazole 1 | 3.72 | 168.45 |
| Benzene thiophene bacterium ester | 8.31 | - |
| Phenylate collarium azoles | 7.42 | - |
Benzene thiophene bacterium ester and Difenoconazole are composite as seen from Table 1, and powdery mildew of cucumber is had to good synergistic effect, and from the proportioning of 1:8 to 8:1, its co-toxicity coefficient is 162.38～279.54, is all greater than 120, and this shows that the two synergistic effect under this proportioning is obvious.Benzene thiophene bacterium ester and Difenoconazole are composite as can be seen from Table 2, to preventing and treating stripe rust of wheat, also have very high synergistic effect, and from the proportioning of 1:8 to 8:1, its co-toxicity coefficient is 172.12～325.00, and the two is composite with the ratio of 3:5, EC
50be only 4.35mg/L, synergistic effect is obvious.Benzene thiophene bacterium ester and Difenoconazole are composite as shown in Table 3, to preventing and treating stripe rust of wheat, have equally very high synergistic effect, co-toxicity coefficient is all greater than 120, and between 168.45～272.63, by the composite growth that can obviously suppress anthrax scab, reduce medicament usage amount.
Bactericidal composition, when practical application, can be made different application forms according to a conventional method, comprises missible oil, suspending agent, wetting powder, water dispersible granules.In preparation, the mass content of the two gross mass of benzene thiophene bacterium ester and Difenoconazole in bactericidal composition is 10%～80%.
Below in conjunction with specific embodiment, the invention will be further described.
Embodiment 1
This example provides a kind of 45% benzene thiophene bacterium ester difenoconazole suspending agent, content meter by weight, its composition of raw materials is: benzene thiophene bacterium ester 5%, Difenoconazole 40%, wetting agent neopelex 3%, dispersant sodium lignin sulfonate 3%, thickener aluminium-magnesium silicate 1%, antifreeze glycol 5%, defoamer silicone oil 1%, water 42%.
The preparation method of suspending agent is: by formula, each material is weighed up to premixed, then add in sand mill sand milling groove, add the bead with material equivalent simultaneously, with 8000r/min speed speed lapping 2h, obtain preparation.
The technical indicator of gained suspending agent is as follows: viscosity 0.5Pas; Suspensibility 91%; PH value 6.0～8.0; Screen analysis (by 75 μ m hole sizers) >=98%; Lasting foaming characteristic (after 1min)≤20mL; Pourability: topple over rear residue≤2.5%, residue≤0.3% after washing; Preparation stabilization after 54 ± 2 ℃ of heat storage 14d, without layering, precipitation, suspensibility >=90.6%; Preparation stabilization after 0 ± 2 ℃ of cold storage 14d, without layering, precipitation, suspensibility >=90%.
Embodiment 2
This example provides a kind of 35% benzene thiophene bacterium ester difenoconazole suspending agent, content meter by weight, its composition of raw materials is: benzene thiophene bacterium ester 5%, Difenoconazole 30%, alkyl polyoxyethylene ether sulfonate 2%, dispersant polycarboxylate sodium salt 5%, thickener xanthans 0.5%, antifreezing agent glycerine 5%, defoamer 1%, water 51.5%.The preparation method of suspending agent is with embodiment 1.
The technical indicator of gained suspending agent is as follows: viscosity 0.5Pas; Suspensibility 93%; PH value 6.0～8.0; Screen analysis (by 75 μ m hole sizers) >=98%; Lasting foaming characteristic (after 1min)≤20mL; Pourability: topple over rear residue≤2.5%, residue≤0.2% after washing; Preparation stabilization after 54 ± 2 ℃ of heat storage 14d, without layering, precipitation, suspensibility >=92.4%; Preparation stabilization after 0 ± 2 ℃ of cold storage 14d, without layering, precipitation, suspensibility >=92.3%.
Embodiment 3
This example provides a kind of 10% benzene thiophene bacterium ester difenoconazole suspending agent, content meter by weight, its composition of raw materials is: benzene thiophene bacterium ester 3.75%, Difenoconazole 6.25%, wetting agent agriculture breast 16003%, dispersant EO-PO block copolymer 4%, thickener aluminium-magnesium silicate 1%, antifreeze glycol 5%, defoamer 1%, water 76%.The preparation method of suspending agent is with embodiment 1.
The technical indicator of gained suspending agent is as follows: viscosity 0.5Pas; Suspensibility 93.4%; PH value 6.0～8.0; Screen analysis (by 75 μ m hole sizers) >=98%; Lasting foaming characteristic (after 1min)≤20mL; Pourability: topple over rear residue≤2.5%, residue≤0.3% after washing; Preparation stabilization after 54 ± 2 ℃ of heat storage 14d, without layering, precipitation, suspensibility >=92.5%; Preparation stabilization after 0 ± 2 ℃ of cold storage 14d, without layering, precipitation, suspensibility >=92%.
Embodiment 4
This example provides a kind of 10% benzene thiophene bacterium ester difenoconazole suspending agent, content meter by weight, its composition of raw materials is: benzene thiophene bacterium ester 6.25%, Difenoconazole 3.75%, wetting agent agriculture breast 16003%, dispersant EO-PO block copolymer 4%, thickener aluminium-magnesium silicate 1.5%, antifreeze glycol 4.5%, defoamer 1%, water 76%.Suspending agent preparation method is with embodiment 1.
The technical indicator of gained suspending agent is as follows: viscosity 0.5Pas; Suspensibility 91%; PH value 6.0～8.0; Screen analysis (by 75 μ m hole sizers) >=99%; Lasting foaming characteristic (after 1min)≤20mL; Pourability: topple over rear residue≤2.0%, residue≤0.2% after washing; Preparation stabilization after 54 ± 2 ℃ of heat storage 14d, without layering, precipitation, suspensibility >=91%; Preparation stabilization after 0 ± 2 ℃ of cold storage 14d, without layering, precipitation, suspensibility >=90%.
Embodiment 5
This example provides a kind of 35% benzene thiophene bacterium ester difenoconazole suspending agent, content meter by weight, its composition of raw materials is: benzene thiophene bacterium ester 30%, Difenoconazole 5%, wetting agent succinic acid dioctyl sodium sulfonate 2%, dispersant polycarboxylate sodium salt 5%, thickener xanthans 0.5%, antifreezing agent glycerine 5%, defoamer 1%, water 51.5%.The preparation method of suspending agent is with embodiment 1.
The technical indicator of gained suspending agent is as follows: viscosity 0.5Pas; Suspensibility 93%; PH value 6.0～8.0; Screen analysis (by 75 μ m hole sizers) >=98%; Lasting foaming characteristic (after 1min)≤20mL; Pourability: topple over rear residue≤2.5%, residue≤0.2% after washing; Preparation stabilization after 54 ± 2 ℃ of heat storage 14d, without layering, precipitation, suspensibility >=92%; Preparation stabilization after 0 ± 2 ℃ of cold storage 14d, without layering, precipitation, suspensibility >=92.1%.
Embodiment 6
This example provides a kind of 45% benzene thiophene bacterium ester difenoconazole suspending agent, content meter by weight, its composition of raw materials is: benzene thiophene bacterium ester 40%, Difenoconazole 5%, wetting agent neopelex 3%, dispersant sodium lignin sulfonate 3%, thickener aluminium-magnesium silicate 1%, antifreeze glycol 5%, defoamer silicone oil 1%, water 42%.The preparation method of suspending agent is with embodiment 1.
The technical indicator of gained suspending agent is as follows: viscosity 0.5Pas; Suspensibility 91.4%; PH value 6.0～8.0; Screen analysis (by 75 μ m hole sizers) >=98%; Lasting foaming characteristic (after 1min)≤20mL; Pourability: topple over rear residue≤2.5%, residue≤0.3% after washing; Preparation stabilization after 54 ± 2 ℃ of heat storage 14d, without layering, precipitation, suspensibility >=91%; Preparation stabilization after 0 ± 2 ℃ of cold storage 14d, without layering, precipitation, suspensibility >=91.2%.
Embodiment 7
This example provides a kind of 10% benzene thiophene bacterium ester difenoconazole suspending agent, content meter by weight, its composition of raw materials is: benzene thiophene bacterium ester 1%, Difenoconazole 9%, wetting agent agriculture breast 16003%, dispersant EO-PO block copolymer 4%, thickener aluminium-magnesium silicate 1%, antifreeze glycol 4%, defoamer 2%, water 76%.The preparation method of suspending agent is with embodiment 1.
The technical indicator of gained suspending agent is as follows: viscosity 0.5Pas; Suspensibility 90.6%; PH value 6.0～8.0; Screen analysis (by 75 μ m hole sizers) >=95%; Lasting foaming characteristic (after 1min)≤20mL; Pourability: topple over rear residue≤5.5%, residue≤0.5% after washing; After 54 ± 2 ℃ of heat storage 14d, preparation has slight layering, suspensibility >=90.2%; After 0 ± 2 ℃ of cold storage 14d, preparation has slight layering, suspensibility >=90%.
Embodiment 8
This example provides a kind of 72% benzene thiophene bacterium ester difenoconazole water dispersible granule, content meter by weight, its composition of raw materials is: benzene thiophene bacterium ester 8%, Difenoconazole 64%, wetting agent naphthyl sulfonate 1%, dispersant EO-PO block copolymer 3%, alkylphenol ethoxylate 3%, gum Arabic 2%, kaolin 14%, water 5%.
The preparation method of water dispersible granules is: by formula, each material is weighed up to premixed, then mix, through airslide disintegrating mill, pulverize, then add in kneader and mediate, extruder grain, is drying to obtain.
The technical indicator of gained water dispersible granules is as follows: suspensibility >=91.6%; Wetting time≤50s; Moisture≤3.0%; PH value 6.0～8.0; Screen analysis (by 75 μ m hole sizers) >=98%; Lasting foaming characteristic (after 1min)≤25mL; Residue≤0.3% after washing; Particle size range (0.8mm～1.5mm) >=96%; Preparation stabilization after 54 ± 2 ℃ of heat storage 14d, without layering, precipitation, suspensibility >=91%; Preparation stabilization after 0 ± 2 ℃ of cold storage 14d, without layering, precipitation, suspensibility >=90%.
Embodiment 9
This example provides a kind of 30% benzene thiophene bacterium ester difenoconazole water dispersible granule, content meter by weight, its composition of raw materials is: benzene thiophene bacterium ester 4.3%, Difenoconazole 25.7%, wetting agent lauryl sodium sulfate 2%, dispersant EO-PO block copolymer 5%, alkylphenol ethoxylate 3%, gum Arabic 2%, kaolin 49%, diatomite 5%, water 4%.The preparation method of water dispersible granules is with embodiment 8.
The technical indicator of gained water dispersible granules is as follows: suspensibility >=91.2%; Wetting time≤50s; Moisture≤3.0%; PH value 6.0～8.0; Screen analysis (by 75 μ m hole sizers) >=98%; Lasting foaming characteristic (after 1min)≤25mL; Residue≤0.3% after washing; Particle size range (0.8mm～1.5mm) >=96%; Preparation stabilization after 54 ± 2 ℃ of heat storage 14d, without layering, precipitation, suspensibility >=91%; Preparation stabilization after 0 ± 2 ℃ of cold storage 14d, without layering, precipitation, suspensibility >=90%.
Embodiment 10
This example provides a kind of 30% benzene thiophene bacterium ester difenoconazole water dispersible granule, content meter by weight, its composition of raw materials is: benzene thiophene bacterium ester 4.3%, Difenoconazole 25.7%, octadecyl sodium succinate 1%, dispersant EO-PO block copolymer 3%, alkylphenol ethoxylate 3%, gum Arabic 2%, kaolin 14%, bentonite 42%, water 5%.The preparation method of water dispersible granules is with embodiment 8.
The technical indicator of gained water dispersible granules is as follows: suspensibility >=91.5%; Wetting time≤50s; Moisture≤3.0%; PH value 6.0～8.0; Screen analysis (by 75 μ m hole sizers) >=98%; Lasting foaming characteristic (after 1min)≤25mL; Residue≤0.3% after washing; Particle size range (0.8mm～1.5mm) >=96%; Preparation stabilization after 54 ± 2 ℃ of heat storage 14d, without layering, precipitation, suspensibility >=91.2%; Preparation stabilization after 0 ± 2 ℃ of cold storage 14d, without layering, precipitation, suspensibility >=90%.
Embodiment 11
This example provides a kind of 40% benzene thiophene bacterium ester difenoconazole water dispersible granule, content meter by weight, its composition of raw materials is: benzene thiophene bacterium ester 15%, Difenoconazole 25%, wetting agent lauryl sodium sulfate 1%, lignosulfonates 3%, alkylphenol ethoxylate 3%, gum Arabic 2%, kaolin 14%, bentonite 30%, water 7%.The preparation method of water dispersible granules is with embodiment 8.
The technical indicator of gained water dispersible granules is as follows: suspensibility >=91.6%; Wetting time≤50s; Moisture≤3.0%; PH value 6.0～8.0; Screen analysis (by 75 μ m hole sizers) >=98%; Lasting foaming characteristic (after 1min)≤25mL; Residue≤0.3% after washing; Particle size range (0.8mm～1.5mm) >=96%; Preparation stabilization after 54 ± 2 ℃ of heat storage 14d, without layering, precipitation, suspensibility >=91.1%; Preparation stabilization after 0 ± 2 ℃ of cold storage 14d, without layering, precipitation, suspensibility >=90%.
Embodiment 12
This example provides a kind of 40% benzene thiophene bacterium ester difenoconazole water dispersible granule, content meter by weight, its composition of raw materials is: benzene thiophene bacterium ester 25%, Difenoconazole 15%, wetting agent naphthyl sulfonate 3%, dispersant sodium naphthalene sulfonate formaldehyde 7%, alkylphenol ethoxylate 3%, gum Arabic 2%, kaolin 14%, bentonite 25%, water 6%.The preparation method of water dispersible granules is with embodiment 8.
The technical indicator of gained water dispersible granules is as follows: suspensibility >=92.2%; Wetting time≤50s; Moisture≤3.0%; PH value 6.0～8.0; Screen analysis (by 75 μ m hole sizers) >=98%; Lasting foaming characteristic (after 1min)≤25mL; Residue≤0.3% after washing; Particle size range (0.8mm～1.5mm) >=96%; Preparation stabilization after 54 ± 2 ℃ of heat storage 14d, without layering, precipitation, suspensibility >=91.2%; Preparation stabilization after 0 ± 2 ℃ of cold storage 14d, without layering, precipitation, suspensibility >=90%.
Embodiment 13
This example provides a kind of 28% benzene thiophene bacterium ester difenoconazole water dispersible granule, content meter by weight, its composition of raw materials is: benzene thiophene bacterium ester 24%, Difenoconazole 4%, wetting agent lignosulfonates 1%, dispersant polycarboxylate 3%, alkylphenol ethoxylate 3%, gum Arabic 2%, kaolin 20%, bentonite 40%, water 3%.The preparation method of water dispersible granules is with embodiment 8.
The technical indicator of gained water dispersible granules is as follows: suspensibility >=91.6%; Wetting time≤50s; Moisture≤3.0%; PH value 6.0～8.0; Screen analysis (by 75 μ m hole sizers) >=98%; Lasting foaming characteristic (after 1min)≤25mL; Residue≤0.3% after washing; Particle size range (0.8mm～1.5mm) >=96%; Preparation stabilization after 54 ± 2 ℃ of heat storage 14d, without layering, precipitation, suspensibility >=91%; Preparation stabilization after 0 ± 2 ℃ of cold storage 14d, without layering, precipitation, suspensibility >=90%.
Embodiment 14
This example provides a kind of 60% benzene thiophene bacterium ester difenoconazole water dispersible granule, content meter by weight, its composition of raw materials is: benzene thiophene bacterium ester 53.34%, Difenoconazole 6.64%, wetting agent naphthyl sulfonate 1%, dispersant polyaryl phenol ethylization phosphate 3%, alkylphenol ethoxylate 3%, gum Arabic 2%, kaolin 28%, water 3%.The preparation method of water dispersible granules is with embodiment 8.
The technical indicator of water dispersible granules is as follows: suspensibility >=91.5%; Wetting time≤50s; Moisture≤3.0%; PH value 6.0～8.0; Screen analysis (by 75 μ m hole sizers) >=98%; Lasting foaming characteristic (after 1min)≤25mL; Residue≤0.3% after washing; Particle size range (0.8mm～1.5mm) >=96%; Preparation stabilization after 54 ± 2 ℃ of heat storage 14d, without layering, precipitation, suspensibility >=91.1%; Preparation stabilization after 0 ± 2 ℃ of cold storage 14d, without layering, precipitation, suspensibility >=90%.
Embodiment 15
This example provides a kind of 15% benzene thiophene bacterium ester Difenoconazole missible oil, content meter by weight, and its composition of raw materials is: benzene thiophene bacterium ester 13.34%, Difenoconazole 1.64%, emulsifier 602#5%, emulsifier 500#2%, emulsifier EL-3602%, solvent naphtha 150#76%.
The preparation method of missible oil: each component of above-mentioned formula is weighed, benzene thiophene bacterium ester and Difenoconazole are used after solvent naphtha 150%# dissolving, with emulsifier mix and blend.
The technical indicator of gained missible oil is as follows: emulsifying dispersivity: the cloud and mist that can automatically be white in color disperses; Emulsion stability: dilute 200 times, 1h emulsion-stabilizing under 25～30 ℃ of conditions; Preparation stabilization after 54 ± 2 ℃ of heat storage 14d, without layering, precipitation; Preparation stabilization after 0 ± 2 ℃ of cold storage 14d, without layering, crystallization; PH value 6～8.
Embodiment 16
This example provides a kind of 30% benzene thiophene bacterium ester Difenoconazole missible oil, content meter by weight, and its composition of raw materials is: benzene thiophene bacterium ester 25.72%, Difenoconazole 4.28%, emulsifier 600 #5%, emulsifier 500#2%, Emulsifier EL-40 2%, solvent naphtha 150#61%.The preparation method of missible oil is with embodiment 15.
The technical indicator of gained missible oil is as follows: emulsifying dispersivity: the cloud and mist that can automatically be white in color disperses; Emulsion stability: dilute 200 times, 1h emulsion-stabilizing under 25～30 ℃ of conditions; Preparation stabilization after 54 ± 2 ℃ of heat storage 14d, without layering, precipitation; After 0 ± 2 ℃ of cold storage 14d, preparation stabilization is without layering, crystallization; PH value 6～8.
Embodiment 17
This example provides a kind of 40% benzene thiophene bacterium ester Difenoconazole missible oil, content meter by weight, and its composition of raw materials is: benzene thiophene bacterium ester 25%, Difenoconazole 15%, emulsifier 600 #5%, emulsifier 500#2%, emulsifier 600 22%, solvent naphtha 150#51%.The preparation method of missible oil is with embodiment 15.
The technical indicator of gained missible oil is as follows: emulsifying dispersivity: the cloud and mist that can automatically be white in color disperses; Emulsion stability: dilute 200 times, 1h emulsion-stabilizing under 25～30 ℃ of conditions; Preparation stabilization after 54 ± 2 ℃ of heat storage 14d, without layering, precipitation; Preparation stabilization after 0 ± 2 ℃ of cold storage 14d, without layering, crystallization; PH value 6～8.
Embodiment 18
This example provides a kind of 40% benzene thiophene bacterium ester Difenoconazole missible oil, content meter by weight, and its composition of raw materials is: benzene thiophene bacterium ester 15%, Difenoconazole 25%, emulsifier 601#5%, emulsifier 500#2%, Emulsifier O E-62%, solvent naphtha 150#51%.The preparation method of missible oil is with embodiment 15.
The technical indicator of gained missible oil is as follows: emulsifying dispersivity: the cloud and mist that can automatically be white in color disperses; Emulsion stability: dilute 200 times, 1h emulsion-stabilizing under 25～30 ℃ of conditions; Preparation stabilization after 54 ± 2 ℃ of heat storage 14d, without layering, precipitation; Preparation stabilization after 0 ± 2 ℃ of cold storage 14d, without layering, crystallization; PH value 6～8.
Embodiment 19
This example provides a kind of 30% benzene thiophene bacterium ester Difenoconazole missible oil, content meter by weight, and its composition of raw materials is: benzene thiophene bacterium ester 4.28%, Difenoconazole 25.72%, emulsifier 600 #5%, emulsifier 500#2%, agriculture breast 30402%, solvent naphtha 150#61%.The preparation method of missible oil is with embodiment 15.
The technical indicator of gained missible oil is as follows: emulsifying dispersivity: the cloud and mist that can automatically be white in color disperses; Emulsion stability: dilute 200 times, 1h emulsion-stabilizing under 25～30 ℃ of conditions; Preparation stabilization after 54 ± 2 ℃ of heat storage 14d, without layering, precipitation; Preparation stabilization after 0 ± 2 ℃ of cold storage 14d, without layering, crystallization; PH value 6～8.
Embodiment 20
This example provides a kind of 15% benzene thiophene bacterium ester Difenoconazole missible oil, content meter by weight, and its composition of raw materials is: benzene thiophene bacterium ester 1.64%, Difenoconazole 13.36%, emulsifier 600 #5%, emulsifier 500#2%, emulsifier op-10 2%, solvent naphtha 150#76%.The preparation method of missible oil is with embodiment 15.
The technical indicator of gained missible oil is as follows: emulsifying dispersivity: the cloud and mist that can automatically be white in color disperses; Emulsion stability: dilute 200 times, 1h emulsion-stabilizing under 25～30 ℃ of conditions; Preparation stabilization after 54 ± 2 ℃ of heat storage 14d, without layering, precipitation; Preparation stabilization after 0 ± 2 ℃ of cold storage 14d, without layering, crystallization; PH value 6～8.
Embodiment 21
This example provides a kind of 60% benzene thiophene bacterium ester Difenoconazole wetting powder, content meter by weight, its composition of raw materials is: benzene thiophene bacterium ester 10%, Difenoconazole 50%, wetting agent calgon 3%, pull open powder 1%, dispersant sodium lignin sulfonate 3%, fill out a potter's clay surplus and supply 100%.
Preparation method: above-mentioned each component is weighed in proportion, be pulverized and mixed and make through mixed airflow cracker.
The technical indicator of gained wetting powder is as follows: fineness (44 μ m) >=95%, and wetability 1min, suspensibility 86%, moisture 1%, 54 ± 2 ℃ of heat storage stabilities, storage 14d, without caking, active ingredient resolution ratio 3%.
Embodiment 22
This example provides a kind of 72% benzene thiophene bacterium ester Difenoconazole wetting powder, content meter by weight, its composition of raw materials is: benzene thiophene bacterium ester 27%, Difenoconazole 45%, wetting agent calgon 4.5%, pull open powder 1.8%, dispersant sodium lignin sulfonate 4%, fill out a potter's clay surplus and supply 100%.Preparation method is with embodiment 21.
The technical indicator of gained wetting powder is as follows: fineness (44 μ m) >=95%, and wetability 1min, suspensibility 86.5%, moisture 1%, 54 ± 2 ℃ of heat storage stabilities, storage 14d, without caking, active ingredient resolution ratio 3%.
the bactericidal composition of embodiment 1～22 is carried out to field trial, observe the control efficiency to watermelon anthrax.
Test site is suburb, Zhangjiagang, Jiangsu Province, and reagent agent is provided by Jiangsu Qizhou Green Chemical Co., Ltd.
Investigation watermelon anthrax disease index before medicine, in their early stage dispenser for the first time, once, dispenser is 2 times altogether in dispenser in every 10 days.After last dispenser, 7d, 14d and 21d investigate respectively disease index and calculate preventive effect.Result of the test is as shown in table 4.
Table 4 bactericidal composition and single dose control watermelon anthrax field control effectiveness test thereof
As shown in Table 4, after benzene thiophene bacterium ester and Difenoconazole are composite, can effectively control watermelon anthrax, after dispenser, 14d preventive effect is greater than 85%, and control efficiency is all better than benzene thiophene bacterium ester and Difenoconazole single dose, and on target plant growth without impact, without poisoning.
the bactericidal composition of embodiment 1～22 and single dose thereof have been carried out to acute toxic test
In Toxicity Determination test and field trial, determine that benzene thiophene bacterium ester Difenoconazole bactericidal composition has on good preventive effect basis powdery mildew, yellow rust and anthracnose, has carried out acute toxic test.Result of the test is as shown in table 5.
The acute toxicity tests of table 5 bactericidal composition and single dose thereof
As shown in Table 5, than benzene thiophene bacterium ester, difenoconazole technical material, the composite rear acute toxicity to small white mouse, rabbit and cavy of benzene thiophene bacterium ester and Difenoconazole all decreases, to small white mouse Oral toxicity LD
50all>=500, percutaneous toxicity all>=2000, is low toxicity compounds.
The present invention adopts above-mentioned formula combination, can produce obvious synergistic function, field trial show this bactericidal composition to crop safety without poisoning, and composite rear mammal acute toxicity is reduced, has improved safety.Use separately comparison with benzene thiophene bacterium ester, Difenoconazole, expanding prevention spectrum, delays pesticide resistance and produces, and has reduced field pesticides consumption and spraying times, has saved agricultural cost, has better protected crop safety and ecotope.
Above the present invention is described in detail; its object is to allow the personage who is familiar with this art can understand content of the present invention and be implemented; can not limit the scope of the invention with this; and the invention is not restricted to the embodiments described; the equivalence that all Spirit Essences according to the present invention are done changes or modifies, within all should being encompassed in protection scope of the present invention.
Claims (9)
1. a bactericidal composition that contains benzene thiophene bacterium ester and Difenoconazole, it is comprised of active component and pesticidal preparations auxiliary element, it is characterized in that, described active component is comprised of benzene thiophene bacterium ester and Difenoconazole, and the mass ratio of described benzene thiophene bacterium ester and Difenoconazole is 1:8 to 8:1.
2. the bactericidal composition that contains benzene thiophene bacterium ester and Difenoconazole according to claim 1, is characterized in that, the mass ratio of described benzene thiophene bacterium ester and Difenoconazole is 1:6 to 6:1.
3. the bactericidal composition that contains benzene thiophene bacterium ester and Difenoconazole according to claim 2, is characterized in that, the mass ratio of described benzene thiophene bacterium ester and Difenoconazole is 1:4 to 4:1.
4. the bactericidal composition that contains benzene thiophene bacterium ester and Difenoconazole according to claim 3, is characterized in that, the mass ratio of described benzene thiophene bacterium ester and Difenoconazole is 1:2 to 2:1.
5. the bactericidal composition that contains benzene thiophene bacterium ester and Difenoconazole according to claim 4, is characterized in that, the mass ratio of described benzene thiophene bacterium ester and Difenoconazole is 3:5.
6. the bactericidal composition that contains benzene thiophene bacterium ester and Difenoconazole according to claim 1, is characterized in that, the formulation of described bactericidal composition is missible oil, wetting powder, suspending agent or water dispersible granules.
7. the bactericidal composition that contains benzene thiophene bacterium ester and Difenoconazole according to claim 6, is characterized in that, in described formulation, the mass content of the two gross mass of benzene thiophene bacterium ester and Difenoconazole in described bactericidal composition is 10% ~ 80%.
8. the application of the bactericidal composition that contains benzene thiophene bacterium ester and Difenoconazole described in arbitrary claim in the disease of control fruit tree, vegetables and cereal crop in claim 1 to 7.
9. application according to claim 8, is characterized in that, described disease is powdery mildew, yellow rust, anthracnose.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310520873.0A CN103548840B (en) | 2013-10-29 | 2013-10-29 | Sterilization compound containing benzothiostrobin and difenoconazole and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310520873.0A CN103548840B (en) | 2013-10-29 | 2013-10-29 | Sterilization compound containing benzothiostrobin and difenoconazole and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103548840A CN103548840A (en) | 2014-02-05 |
| CN103548840B true CN103548840B (en) | 2014-09-10 |
Family
ID=50003355
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310520873.0A Active CN103548840B (en) | 2013-10-29 | 2013-10-29 | Sterilization compound containing benzothiostrobin and difenoconazole and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103548840B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104054718B (en) * | 2014-06-12 | 2015-06-17 | 南京农业大学 | Method for treating insecticide resistance of sclerotiniose of oilseed rapes |
| CN108064855A (en) * | 2016-11-11 | 2018-05-25 | 华中师范大学 | A kind of composition pesticide and its application containing benzothiostrobin and prothioconazoles |
| CN107094774A (en) * | 2017-05-06 | 2017-08-29 | 佛山市瑞生通科技有限公司 | Synergistic composition containing benzothiostrobin |
| CN107136097A (en) * | 2017-06-07 | 2017-09-08 | 广西南宁乐蕊生物科技有限责任公司 | A kind of bactericidal composition |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102005015677A1 (en) * | 2005-04-06 | 2006-10-12 | Bayer Cropscience Ag | Synergistic fungicidal drug combinations |
| CN103027054B (en) * | 2011-12-27 | 2014-05-28 | 湖北仙隆化工股份有限公司 | Composition containing benzothiostrobin and azoxystrobin and having bactericidal effect |
-
2013
- 2013-10-29 CN CN201310520873.0A patent/CN103548840B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN103548840A (en) | 2014-02-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101715777B (en) | Pesticide composition containing ethirimol and thiophanate-methyl, and application thereof | |
| CN102165956B (en) | Pesticide composite containing bispyribac-sodium and penoxsulam and application thereof | |
| CN101999356B (en) | Sterilization composition containing fluopicolide and metalaxyl and application thereof | |
| CN101595883A (en) | A kind of composition pesticide and application thereof | |
| CN103548840B (en) | Sterilization compound containing benzothiostrobin and difenoconazole and application thereof | |
| CN103598198B (en) | A kind of triazole type and nicotine compound compound seed treating agent and preparation method thereof | |
| CN104054712B (en) | Sterilization composition containing boscalid | |
| CN103355309B (en) | Sterilization composition and preparation thereof | |
| CN101697722B (en) | Famoxadone-containing bactericidal composition | |
| CN102835399B (en) | Metrafenone-containing bactericidal composition | |
| CN105230642A (en) | Bactericidal composition containing isopyrazam and application thereof | |
| CN106135232B (en) | bactericidal composition | |
| CN112602720A (en) | Bactericide composition containing triphenyltin acetate and difenoconazole and preparation method thereof | |
| CN101632374B (en) | Pesticide composition and application thereof | |
| CN104068033B (en) | Fungicidal composition containing boscalid | |
| CN109526958A (en) | Microbicide compositions containing rheum emodin and metconazole | |
| CN114190392B (en) | Sterilization composition containing difenoconazole and ketoconazole and application thereof | |
| CN104542626B (en) | A kind of containing methylsulfonyl bacterium azoles and the complex composition of CGA-173506 and preparation | |
| CN107853309A (en) | A kind of composition pesticide comprising folpet and dimethomorph | |
| CN104705344A (en) | Sulfur-containing bactericidal agent composition and application thereof | |
| CN106719647A (en) | A kind of microbicide compositions comprising anthraquinone analog compound and metrafenone | |
| CN101755766A (en) | Bactericidal composition having active ester | |
| CN104904731B (en) | Sterilization composition containing ZJ5337 | |
| CN104488887A (en) | Bactericidal composition containing famoxadone and boscalid | |
| CN108684676A (en) | A kind of bactericidal composition containing volution bacterium amine and fludioxonil |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |