CN103518601B - A kind of method being applicable to tea tree fringe bar incubator and cultivating - Google Patents
A kind of method being applicable to tea tree fringe bar incubator and cultivating Download PDFInfo
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- CN103518601B CN103518601B CN201310486894.5A CN201310486894A CN103518601B CN 103518601 B CN103518601 B CN 103518601B CN 201310486894 A CN201310486894 A CN 201310486894A CN 103518601 B CN103518601 B CN 103518601B
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Abstract
Be applicable to the method that tea tree fringe bar incubator is cultivated, belong to technical field of tea tree cultivation.It is characterized in that comprising the following steps: 1) incubator prepares: arrange illumination box, light application time is 14-18h/ days, and intensity of illumination is 90-100%; Humidity 70-85%, incubator temperature is 5-20 DEG C of constant temperature; 2) nutrient solution: culture fluid adopts 1/2MS medium, cultivates for tea tree fringe bar after high-temperature sterilization cooling; 3) fringe bar prepares: be inserted in slot culture in glassware after cutting the process of tea tree fringe bar; 4) incubation: often change culture fluid once in 2-3 days, namely can be observed the impact that temperature is sprouted tea shoot after 4-5 days.A kind of above-mentioned method being applicable to tea tree fringe bar incubator and cultivating, having convenient, fast, reproducible, reliability high, is the Perfected process that research environment, nutrient etc. affect tea leaf quality.
Description
Technical field
The invention belongs to technical field of tea tree cultivation, be specially a kind of method being applicable to tea tree fringe bar incubator and cultivating.
Background technology
The features such as tea tree is a kind of perennial woody plant, has body large, indoor cultivation difficulty.Therefore the impact of the environmental factors such as temp. and humidity on tea tree sprouting, bud-leaf quality is difficult to make first hand study.Mostly past correlative study is that for many years, how the meteorological data of quality comparison data and the correspondence utilizing tealeaves goes to infer that environment is on the impact of tea leaf quality, but this round-about way exists features such as influence factor is many, reliability is not strong.Utilize incubator directly to carry out tea tree basin alms bowl to cultivate and then there is incubator finite volume, test process is few, and tea tree individual difference is large, the problems such as wattles difference is large in same strain tea tree, and therefore repeatability, reliability are also all poor.
Summary of the invention
For the above-mentioned problems in the prior art, the object of the invention is to design provides a kind of technical scheme being applicable to the method that tea tree fringe bar incubator is cultivated, having convenient, fast, reproducible, reliability high, is the Perfected process that research environment, nutrient etc. affect tea leaf quality.
A kind of described method being applicable to tea tree fringe bar incubator and cultivating, is characterized in that comprising the following steps:
1) incubator prepares: arrange illumination box, light application time is 14-18h/ days, and intensity of illumination is 90-100%; Humidity 70-85%, incubator temperature is 5-20 DEG C of constant temperature;
2) nutrient solution: adopt jug to load culture fluid, culture fluid adopts 1/2MS medium, and the concrete composition of culture fluid is NH
4nO
3825mg/L, KNO
3950mg/L, CaCl
22H
2o220mg/L, MgSO
47H
2o185mg/L, KH
2pO
4350mg/L, KI0.4mg/L, H
3bO
33.1mg/L, MnSO
44H
2o11.2mg/L, ZnSO
47H
2o4.3mg/L, Na
2mnO
42H
2o0.13mg/L, CuSO
45H
2o0.013mg/L, CoCl
26H
2o0.013mg/L, FeSO
47H
2o13.9mg/L, Na
2-EDTA2H
2o19.7mg/L, after culture fluid prepares, 110 degree of-130 degree high-temperature sterilization, cultivates for tea tree fringe bar after cooling completely;
3) fringe bar prepare: spring tea shoot by the sprouting stage, clip growth conditions uniformity, healthy, without the tea tree fringe bar of damage by disease and insect, length is about 40-50cm, extract the female leaf of 3-4 sheet bottom fringe bar and corresponding bud, fringe bar base portion 70-75% alcohol washes 2-3 time, then clean 2-3 time with distilled water, fringe bar is inserted in jug, each jug cuttings bar 8-12 branch;
4) incubation: often change culture fluid once in 2-3 days, namely can be observed the impact that temperature is sprouted tea shoot after 4-5 days, takes tea shoot in test process, takes fresh weight, dry weight, mensuration catechin component respectively for 4-5 days and 8-10 days respectively.
A kind of described method being applicable to tea tree fringe bar incubator and cultivating, is characterized in that in step 1): light application time is 15-16h/ days.
A kind of described method being applicable to tea tree fringe bar incubator and cultivating, is characterized in that in step 1): intensity of illumination is 92-96%.
A kind of described method being applicable to tea tree fringe bar incubator and cultivating, is characterized in that in step 1): humidity 75-80%.
A kind of above-mentioned method being applicable to tea tree fringe bar incubator and cultivating, there is convenient, fast, reproducible, reliability high, the conclusion of correlative study can remove the interference of other influences factor as much as possible, result directly, reliably, is the Perfected process that research environment, nutrient etc. affect tea leaf quality.
Embodiment
Below in conjunction with specific embodiment and corresponding test data, the invention will be further described.
Embodiment 1:
1) incubator prepares: arrange two illumination boxs, light application time is 16h/ days, and intensity is 100%; Humidity 75%, temperature two incubators are respectively 8 DEG C of constant temperature and 18 DEG C of constant temperature culture;
2) nutrient solution: adopt 500ml slot plastic bottle for loading culture fluid, culture fluid adopts 1/2MS medium, and concrete composition is NH
4nO
3825, KNO
3950, CaCl
22H
2o220, MgSO
47H
2o185, KH
2pO
4350, KI0.4, H
3bO
33.1, MnSO
44H
2o11.2, ZnSO
47H
2o4.3, Na
2mnO
42H
2o0.13, CuSO
45H
2o0.013, CoCl
26H
2o0.013, FeSO
47H
2o13.9, Na
2-EDTA2H
2o19.7, unit is mg/L, and each slot plastic bottle puts culture fluid 350ml, 120 degree of high-temperature sterilizations after preparing, and cultivates for tea tree fringe after cooling completely;
3) fringe bar prepare: spring tea shoot by the sprouting stage, clip growth conditions uniformity, healthy, without the tea tree fringe bar of damage by disease and insect, length is about 45cm, extract the female leaf of 3-4 sheet bottom fringe bar and corresponding bud, fringe bar base portion 75% alcohol washes 2 times, then clean 2 times with distilled water, fringe bar is inserted in jug, each jug cuttings bar 10;
4) incubation: change culture fluid once in every 2 days.Namely can be observed in test process the impact that temperature sprouts tea shoot after 4 days, within 4 days and 8 days, respectively tea shoot is taken in test process, take fresh weight and dry weight, mensuration catechin component.
Result of the test finds: process 4 days and 8 days, and cultivate tealeaves fresh weight comparatively 8 DEG C of culture and improvement 76% and 37% for 18 DEG C, dry weight improves 27% and 55%, illustrates that high temperature promotes that tealeaves is sprouted and dry-matter accumulation; Catechin composition analysis finds, 3 kinds of catechin composition content temperature influences are larger, wherein cultivate 4 days and tealeaves EGC content comparatively 8 DEG C of cultivation declines 33% and 71% in 8 days for 18 DEG C, EC content rises 181% and 295%, GCG content have dropped 16% and 86%, illustrates that temperature has remarkable impact to these 3 kinds of catechin compositions, and the coefficient of variation between this research each sample repeats all is less than 10%, having repeatability and reliability preferably, is the Perfected process that research environment, nutrient etc. affect tea leaf quality.
In above-described embodiment 1 step 1): light application time can be 14h/ days, 15h/ days, 18h/ days, and intensity of illumination is 90%, intensity of illumination is 95%, intensity of illumination is 100%; Humidity 70%, humidity 80%, humidity 85%; Incubator temperature can according to studying any one thermostat temperature needing to be set between 5-20 DEG C, and the other the same as in Example 1, also can reach beneficial effect of the present invention.
Claims (3)
1. be applicable to the method that tea tree fringe bar incubator is cultivated, it is characterized in that comprising the following steps:
1) incubator prepares: arrange illumination box, light application time is 15-18h/ days, and intensity of illumination is 90-100%; Humidity 70-85%, incubator temperature is 5-20 DEG C of constant temperature;
2) nutrient solution: adopt jug to load culture fluid, culture fluid adopts 1/2MS medium, and the concrete composition of culture fluid is NH
4nO
3825mg/L, KNO
3950mg/L, CaCl
22H
2o220mg/L, MgSO
47H
2o185mg/L, KH
2pO
4350mg/L, KI0.4mg/L, H
3bO
33.1mg/L, MnSO
44H
2o11.2mg/L, ZnSO
47H
2o4.3mg/L, Na
2mnO
42H
2o0.13mg/L, CuSO
45H
2o0.013mg/L, CoCl
26H
2o0.013mg/L, FeSO
47H
2o13.9mg/L, Na
2-EDTA2H
2o19.7mg/L, after culture fluid prepares, 110 degree of-130 degree high-temperature sterilization, cultivates for tea tree fringe bar after cooling completely;
3) fringe bar prepare: spring tea shoot by the sprouting stage, clip growth conditions uniformity, healthy, without the tea tree fringe bar of damage by disease and insect, length is 40-50cm, extract the female leaf of 3-4 sheet bottom fringe bar and corresponding bud, fringe bar base portion 70-75% alcohol washes 2-3 time, then clean 2-3 time with distilled water, fringe bar is inserted in jug, each jug cuttings bar 8-12 branch;
4) incubation: often change culture fluid once in 2-3 days, namely can be observed the impact that temperature is sprouted tea shoot after 4-5 days, takes tea shoot in test process, takes fresh weight, dry weight, mensuration catechin component respectively for 4-5 days and 8-10 days respectively.
2. as claimed in claim 1 a kind of be applicable to tea tree fringe bar incubator cultivate method, it is characterized in that in step 1): intensity of illumination is 92-96%.
3. as claimed in claim 1 a kind of be applicable to tea tree fringe bar incubator cultivate method, it is characterized in that in step 1): humidity 75-80%.
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| CN201310486894.5A CN103518601B (en) | 2013-10-17 | 2013-10-17 | A kind of method being applicable to tea tree fringe bar incubator and cultivating |
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| CN103518601B true CN103518601B (en) | 2015-12-23 |
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| CN101773045A (en) * | 2010-02-26 | 2010-07-14 | 李子辉 | Complete oil-tea camellia wildwood variety improvement method |
| CN101946661A (en) * | 2010-07-30 | 2011-01-19 | 溧阳市天目湖茶叶研究所 | Industrial fast breeding method for white tea |
| CN102696480A (en) * | 2012-05-07 | 2012-10-03 | 广西壮族自治区林业科学研究院 | Method for culturing aseptic oil tea buds in test tube by using sand matrix |
| CN102934603A (en) * | 2012-10-30 | 2013-02-20 | 安徽农业大学 | Asexual rapid propagation method of tea trees and root taking culture solution |
-
2013
- 2013-10-17 CN CN201310486894.5A patent/CN103518601B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101773045A (en) * | 2010-02-26 | 2010-07-14 | 李子辉 | Complete oil-tea camellia wildwood variety improvement method |
| CN101946661A (en) * | 2010-07-30 | 2011-01-19 | 溧阳市天目湖茶叶研究所 | Industrial fast breeding method for white tea |
| CN102696480A (en) * | 2012-05-07 | 2012-10-03 | 广西壮族自治区林业科学研究院 | Method for culturing aseptic oil tea buds in test tube by using sand matrix |
| CN102934603A (en) * | 2012-10-30 | 2013-02-20 | 安徽农业大学 | Asexual rapid propagation method of tea trees and root taking culture solution |
Non-Patent Citations (2)
| Title |
|---|
| 水培茶树对铅的吸收与累积特性研究;赵先明等;《茶叶科学》;20111231;第31卷(第03期);237-246 * |
| 茶树无性系良种"二段法"快繁育苗技术;张强等;《中国茶叶》;20081231(第04期);26-27 * |
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