CN102415335B - Manufacture method of angiopteris esculenta tube flowers - Google Patents
Manufacture method of angiopteris esculenta tube flowers Download PDFInfo
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Abstract
The invention relates to a manufacture method of angiopteris esculenta tube flowers. Tubercles of angiopteris esculenta are used as explants, budlets are produced through the induction in a micropropagation culture medium, the budlets are inoculated into a glass bottle with color culture media to be cultured, and the angiopteris esculenta tube flowers with the ornamental value are obtained. The angiopteris esculenta tube flowers have the advantages that the micropropagation effect is good, the ornamental period is long, the environmental-friendly effect is high, and the economic and social benefits are high.
Description
Technical field
The invention belongs to field of plant tissue culture technique, relate to the cultivation method of a kind of kwan-yin seat lotus, particularly a kind of preparation method of angiopteris esculenta tube flowers.
Background technology
Kwan-yin seat lotus (
Sempervivum tectorum), claim again houseleek, Buddhist seat lotus flower etc., be Crassulaceae Sempervivum ornamental foliage plant, plant is the short shape of growing thickly, the blade screw shape is arranged in lotus throne shape leaf dish, its diameter from 3 centimetres until more than 15 centimetres, fleshy leaf cochlear or long inverted ovoid, the top point, leaf surface is had white powder or crinosity, and Ye Seyi kind difference has to russet from grayish green, yellowish green, dark green, and the point on leaf top has green, redness, brown or purple, its size is also different, the sawtooth that the leaf margin tool is fine and closely woven.Its strain shape is dignified, and just as a lotus flower in full bloom, the leaf look is full of variety, and different mauve blade tip is very unique and different according to kind, is fit to do middle-size and small-size potted plant or combined pot culture.Well-developed plant is being understood below large lotus throne and gives birth to one and enclose little lotus throne, and is very lovely.Annual spring end also can be extracted from the leafage bottom redness of similar bracketplant out and be walked stem, and walking stem front end length has the little leafage of rosette-stape, has very high ornamental value.In addition, kwan-yin seat lotus also has higher medical value and health-care effect.Extract is used for the treatment of throat ulcer, mouth disease, and bronchitis, and the disease of skin such as burn, scald, bite by mosquitos, its health-care effect is mainly reflected in its nourishing to skin, liver protection effect and makes tea and drink the effect of refreshing oneself.
The test tube flowers are to utilize plant tissue culture technique, under the manual control condition of laboratory, prior cultured plantlet are planted in the transparent vessel with better sight the general name of a series of mini microphytes of control growth in seal cavity.The test tube flowers are referred to as again " angel's greenhouse " or " mini colored room ".The test tube flowers because of it has need not water and fertilizer management, the advantage such as the time of viewing and admiring is long, be subject in recent years urbanite's favor, thereby brought huge business potential also for the test tube flowers.Although existing many about the report of test tube flower at present, the vegetation type that is applied to the making of test tube flower is also very limited.Because kwan-yin seat lotus has the higher characteristic of viewing and admiring, therefore, the making of carrying out the test tube flowers take kwan-yin seat lotus as material seems necessary.CN1300537A discloses a kind of method for quickly breeding of Alocasia amazonica, its technical essential is that the tubercle of getting Alocasia amazonica is explant, induce the generation callus, induce again Multiple Buds, be cut into simple bud and continue in medium, to enlarge continuously cultivation, just can breed in a short time a large amount of seedling, bottle outlet is transplanted upper basin and is cultivated into commodity.It is the seedling of aroid Alocasia amazonica that the method obtains, and the upper basin of seedling bottle outlet transplanting that need to breeding is good is cultivated, and can not obtain mini miniature test tube flowers.
In sum, the preparation method of angiopteris esculenta tube flowers has no report in the prior art at present.
Summary of the invention
In order to enrich test tube flowers material, the object of the invention is to the best to kwan-yin seat lotus and expand numerous condition and explore, thereby a kind of preparation method of angiopteris esculenta tube flowers is provided.
The objective of the invention is to test like this:
A kind of preparation method of angiopteris esculenta tube flowers take the tubercle of kwan-yin seat lotus as explant, is induced in expanding breeding culture medium and is produced budlet, budlet is connected in the vial that colored medium is housed cultivates the angiopteris esculenta tube flowers that acquisition can Gong view and admire.
Described explant obtains as follows: kwan-yin seat lotus is cut off from the bottom, remove stem blade on every side, make it expose one section stem, keep terminal bud 6-8 blade on every side, walk stem and also do similar processing; With flowing water flushing, wash dust off and be clipped in impurity in the blade, with distillation washing 2-3 time, use again 70% alcohol-pickled 2 minutes, constantly shake; Outwell alcohol after two minutes, with 30% 84 thimerosals sterilization 30 minutes, also constantly shake during sterilizing with 84 thimerosals; Outwell 84 thimerosals after 30 minutes, use sterile water wash 3-4 time; The stem section of turning white in the budlet bottom of cleaning is cut, and sagging leaf is cut.
The preparation method of described angiopteris esculenta tube flowers, wherein expand breeding culture medium and be: MS+0.6 mg/L NAA, agar 8 g/L, sugared 40 g/L, pH value are 5.8-6.2.Sugar is preferably white granulated sugar as the carbon source in the medium.
The preparation method of described angiopteris esculenta tube flowers, wherein induce the condition of cultivation to be in expanding breeding culture medium: temperature is 22 ± 1 ℃, illumination every day 10 hours, 40 days subcultures are once.
The preparation method of described angiopteris esculenta tube flowers, wherein colored medium is: MS+ agar 8 g/L, sugared 40 g/L, pigment 0.2-25g/L, pH value are 5.8-6.2.
Described pigment is preferably lemon yellow, greyish purple, apple green or carmine.
The present invention is Anywhere in the described medium, wherein:
MS is international medium, and its composition and compound method are referring to document (Tan Wencheng, Dai Cegang chief editor, ornamental plants tissue culture technique, Beijing: Chinese state forestry publishing house, 1991)
BA also is abbreviated as 6-BA, and chemical composition is 6-benzyl aminopurine, is configured to the high concentration mother liquor during use, i.e. 0.5mg/mL.Concrete configuration method: take by weighing 50 milligrams of 6-BA, with a small amount of 95% alcohol dissolving, then be settled to 100 milliliters with distilled water first, can use.
The chemical composition of NAA is methyl α-naphthyl acetate, is configured to the mother liquor of 0.5mg/mL during use.Concrete configuration method: take by weighing 50 milligrams of NAA, with a small amount of 95% alcohol dissolving, then be settled to 100 milliliters with distilled water first, can use.
The preparation method of the angiopteris esculenta tube flowers that compared with prior art, the present invention relates to has following advantage and significant progressive:
(1) expansion is numerous effective.Kwan-yin seat lotus explant adds NAA in MS obtains and expands preferably numerous effect, expands numerous multiple and reaches 3.81.
(2) angiopteris esculenta tube flowers viewing period is longer.Because kwan-yin seat lotus growth fraction is slower, it can be survived more than 4 months in little blake bottle, if culture vessel increases, medium content increases, and will prolong viewing and admiring the time of kwan-yin seat lotus.
(3) feature of environmental protection is strong.The sterilization of 84 thimerosals is adopted in the sterilization of kwan-yin seat lotus stem section, medium all is medium commonly used, and the color of colored medium all is with the food colour allotment, also can not bring the pollutions such as earth insect to environment, can beautify indoor environment, bring again the enjoyment of natural beauty to people.
(4) economic benefit and social benefit are high.The test tube flowers manufacture craft of kwan-yin seat lotus is simple, and the space that takies is little, and the time of viewing and admiring is long, and the test tube flowers all grow under aseptic condition, need not to water, the management such as fertilising, has economic benefit and the social benefit of highly significant.
Embodiment
Below be the specific embodiment that the present invention relates to, technical scheme of the present invention is done further the description, but protection scope of the present invention is not limited to these embodiment.Every do not deviate from the change of the present invention design or be equal to substitute include within protection scope of the present invention.
The best breeding culture medium that expands of this experimental exploring kwan-yin seat lotus obtains a large amount of kwan-yin seat lotus aseptic seedling, and makes the test tube flowers as material, and further developing of the horn of plenty test tube industry of flowers and plants lays the foundation.
1 materials and methods
1.1 experiment material
Kwan-yin seat lotus: buy in Wuhan, Hubei the large market of male Chu Huahui.
1.2 experimental technique
1.2.1 the sterilization of explant and the acquisition of aseptic seedling
Kwan-yin seat lotus is cut off from the bottom, remove stem blade on every side, make it expose one section stem, keep terminal bud 6-8 blade on every side, walk stem and also do similar processing; With flowing water flushing, wash dust off and be clipped in impurity in the blade, with distillation washing 2-3 time, use again 70% alcohol-pickled 2 minutes, constantly shake; Outwell alcohol after two minutes, half of materials is with 20% 84 thimerosals (commercially available, as to originate in Wenzhou) sterilization 30 minutes, and second half is with 30% 84 thimerosal sterilizations 30 minutes, also constantly shakes during sterilizing with 84 thimerosals; Outwell 84 thimerosals after 30 minutes, use sterile water wash 3-4 time, until do not occur till the foam in the water.
The stem section of turning white in the budlet bottom of cleaning is cut, and sagging leaf is cut, in the access MS medium, and every bottle graft one strain, two kinds of each 15 strains of sterilising conditions are 22 ± 1 ℃ in temperature, cultivate under 10 hours the condition of illumination every day.Observe and record pollution condition after seven days, 40 days subcultures once.
Different white granulated sugar concentration are to kwan-yinSeat
Lotus is expanded the impact of numerous coefficient
With kwan-yin seat lotus aseptic seedling respectively subculture in MS, add in the medium of variable concentrations white granulated sugar, i.e. MS1, white granulated sugar concentration is 30 g/L, MS2 is that 35 g/L and MS3 are 40 g/L, 36 bottles every group, every bottle of 1 strain repeats minutes for three times, concrete prescription sees Table 1.Added up afterwards the budlet number that every strain material newly grows in 40 days, calculate and expand numerous multiple.
Different B A concentration is to kwan-yinSeat
Lotus is expanded the impact of numerous multiple
Add aseptic seedling difference subculture in the 6-BA medium of variable concentrations to the MS medium, be MB1:MS+0.3 mg/L NAA+0.5 mg/L 6-BA, NB2:MS+0.3 mg/L NAA+1.0 mg/L 6-BA, MB3:MS+0.3 mg/L NAA+1.5 mg/L 6-BA, 60 bottles of every winding kinds, every bottle graft kind 1 strain, minute three repetitions, prescription MN1 is control group, and concrete prescription sees Table 3.Added up afterwards the numerous multiple of budlet number (not comprising the budlet that inoculation is entered, i.e. terminal bud) calculating expansion that every strain kwan-yin seat lotus newly grows in 40 days.
Different N AA concentration is to kwan-yinSeat
Lotus is expanded the impact of numerous multiple
Add aseptic seedling difference subculture in the NAA medium of variable concentrations to the MS medium, be MN1:MS+0.3 mg/L NAA, MN2:MS+0.6 mg/L NAA, MN3:MS+0.9 mg/L NAA, each 45 bottles, every bottle graft kind 1 strain, minute three repetitions, concrete prescription sees Table 2, and prescription MS3 is the control group of this experimental design.Added up afterwards the budlet number that every strain material newly grows in 40 days, the numerous multiple of expansion of kwan-yin seat lotus in more every group of medium.
More than three kinds of regulate and control methods when subculture, all to cut first the several blades in kwan-yin seat lotus bottom, expose one section about two millimeters stem, insert again in the above different culture media and cultivate.
Condition of culture all is take MS as minimal medium, and except specific (special) requirements, white granulated sugar concentration is 40 g/L, agar 8 g/L, and the pH value is 5.8-6.2,22 ± 1 ℃ of temperature, the photoperiod is 10 hours.
1.3 the making of test tube flowers
1.3.1 the configuration of colored medium
Adding four kinds of different pigments in medium MS is lemon yellow (0.05 g/mL), greyish purple (0.05 g/ mL), apple green (5 g/ mL) and carmine (0.05 g/mL), every liter of medium addition is respectively 4 mL, 4 mL, 5 mL and 8 mL, acquisition has the colored medium of ornamental value, all pigments all are food additives, and environment is not polluted.
The making of test tube flowers
Colored medium is sub-packed in the vial, and each bottle is poured 8 mL medium into, accounts for 2/5 of culture vessel volume, after the sterilization, kwan-yin seat lotus aseptic seedling of diameter about 1.0 cm is connected in the vial every bottle of strain, totally 30 bottles.Observed and recorded kwan-yin seat lotus viewing and admiring the time in vial.
2 results and analysis
2.1 the interpretation of result that aseptic seedling obtains
Alcohol-pickled 2 min with 70%, the identical time of 84 thimerosals sterilization of variable concentrations, the result shows: the pollution rate after 20% the 84 thimerosals sterilization is 80%, and the survival rate of microbiological contamination plant is not 66.7%; With 30% 84 thimerosals sterilization, pollution rate reduces to 60%, and the survival rate of microbiological contamination plant does not descend to some extent yet, is 60%.Explanation, concentration increase along with 84 thimerosals, can reduce the material contamination rate, simultaneously the injury of plant also strengthened to some extent, causing not, the survival rate of microbiological contamination material descends to some extent, but the ratio that the ratio pollution rate that survival rate descends increases is low, therefore can carry out disinfection with 30% 84 thimerosals, can obtain more aseptic seedling.And during with alcohol and 84 sterilization, must constantly shake liquid, so that the abundant cladding material of liquid surface does not allow its surface form bubble and affect Disinfection Effect.
Different sugar concentration is to kwan-yinSeat
Lotus is expanded the interpretation of result that affects of numerous coefficient
In this experimental design scope, along with increasing of white granulated sugar concentration, kwan-yin seat lotus is expanded numerous multiple and increases gradually (table 1), when white granulated sugar concentration is increased to 40 g/L, expand numerous coefficient and increase to 2.71 from 1.30, aobvious between three groups have significant difference, makes granulated sugar concentration clear and kwan-yin seat lotus is expanded numerous multiple appreciable impact is arranged.This experiment maximum concentration is used 40 g/L, expands numerous multiple and still has significant change, and further improves the white granulated sugar concentration gradient in therefore will testing afterwards, in the hope of obtaining the best white granulated sugar concentration of the higher numerous multiple of expansion.
The different white sugar concentration of table 1 expand numerous multiple on Alocasia amazonica impact
| Formula number | White granulated sugar concentration (g/L) | Inoculation number (individual) | Expand numerous multiple (doubly) |
| MS1 | 30 | 27 | 1.30±0.05c |
| MS2 | 35 | 25 | 2.00±0.00b |
| MS3 | 40 | 25 | 2.71±0.04a |
The different The English alphabet differentials of same column different significantly (P＜0.05).Following table together
2.2 different B A concentration is to kwan-yinSeat
Lotus is expanded the result that affects of numerous multiple
As shown in Table 2, after adding 6-BA in the medium, the easier formation callus of kwan-yin seat lotus seedling, in the experimental design scope, do not add the 6-BA control group and compare, the medium that adds 6-BA all can have the material of significant proportion to form callus, and along with the 6-BA Enrichment, the ratio that forms callus increases, and there were significant differences for the callus ratio that forms between each group.The number that control group forms Multiple Buds reaches 100%, and adding 6-BA concentration is 0.5mg/L, when 1.0 mg/L and 1.5 mg/L, the ratio that forms bud is respectively 57.82%, 38.80%, 21.23%, the ratio that forms bud significantly descends, and the ratio that forms callus but significantly increases (table 2).When adding 6-BA in the medium, kwan-yin seat lotus number that not only forms Multiple Buds reduces, even formed Multiple Buds, the budlet number of generation is also more, but the budlet growth is bad, the same spot of iron rust appears in blade, and wilt easily, along with the growth of incubation time, the material that has formed Multiple Buds still further forms cell mass after the dedifferentiation, can't form normal Multiple Buds, be difficult to carry out further successive propagation.Add in the medium of NAA, have root to produce when cultivating 40 days, make the seedling growth more healthy and stronger, attractive in appearance.Therefore, when kwan-yin seat lotus is expanded numerous cultivation, can only regulate and control to obtain normal numerous offspring of expansion with NAA, and should not add 6-BA.
Kwan-yin seat lotus growing state statistical form of table 2 different B A concentration
2.3
Different N AA concentration is to kwan-yinSeat
Lotus is expanded the result that affects of numerous coefficient
Owing to only add NAA in the medium, it is better to expand numerous effect.Therefore this experimental design the NAA of variable concentrations impact that kwan-yin seat lotus is expanded numerous multiple.As can be seen from Table 3, the amount of NAA is larger on the numerous multiple impact of the expansion of kwan-yin seat lotus.Be in the medium of 0.6 mg/L in NAA concentration, kwan-yin seat lotus is expanded numerous multiple maximum and reaches 3.81, has significant difference with control group, when NAA concentration is increased to 0.9mg/L and is reduced to 0.3mg/L, kwan-yin seat lotus is expanded numerous multiple and all obviously reduces, and expands numerous blade and obviously diminishes, and illustrates to expand numerous budlet poor growth, particularly when adding NAA0.3mg/L, expanding numerous multiple contrast group, not add NAA also low.From experiment overall data analysis and observation, to be that 40 g/L and NAA concentration are that the medium of 0.6 mg/L expands numerous plant out more attractive in appearance for white granulated sugar in the medium, it is high to expand numerous multiple, and the budlet growth rate is also very fast, grow into very soon and parent lotus shape plant of a size, be suitable for doing the test tube flowers.
The impact that table 3 different N AA concentration expands numerous multiple to kwan-yin seat lotus
| Formula number | NAA concentration (mg/L) | Inoculation number (individual) | Expand numerous multiple (doubly) |
| MS3(CK) | 0.0 | 25 | 2.71±0.04b |
| MN1 | 0.3 | 39 | 1.82±0.03c |
| MN2 | 0.6 | 30 | 3.81±0.11a |
| MN3 | 0.9 | 31 | 2.77±0.04b |
2.4 the test tube flowers view and admire the result
22 ± 1 ℃ of temperature, to cultivate under 10 hours/day the condition of periodicity of illumination, the test-tube plantlet in the vial can be survived more than 4 months, and during this period, aseptic seedling does not have great changes, poor growth, the long-time cultivation has root to grow, and is suitable for its better existence.
Conclusion and discussion
Kwan-yin seat lotus is a kind of xerophytes, and there are one deck glandular hairs on its fleshy leaf surface, also has one deck cuticula, and the explant sterilization is very very difficult.This experiment adopts alcohol and 84 thimerosals to use simultaneously, the surface tension coefficient of 70% alcohol is little, can arrive leaf the surface carry out disinfection, but, because its penetration power is strong, so soak time is unsuitable long, time is oversize, it is dead because of dehydration to make explant, and kwan-yin seat lotus leaf sheet surface has cuticula, therefore can not affect its vitality with alcohol-pickled 2min.With using again 84 medicining liquid dippings after alcohol-pickled, can make the mobile enhancing of 84 thimerosals, must constantly shake in the immersion process, can make 84 thimerosals and leaf surface fully contact to reach the effect of sterilization.This experimental applications 20% and 30% 84 thimerosals carry out the explant sterilization, the result shows that the high Disinfection Effect of 84 thimerosal concentration is better, but also larger to plant injury, the terminal bud explant of kwan-yin seat lotus reduces with 30% 84 thimerosals sterilization after stain rate and also not obvious to the explant injury, therefore can adopt 30% 84 medicining liquid dipping 30min. to carry out disinfection and obtain certain explant.But the pollution rate of this scheme still has 60%.From document as can be known, it is best to use certain density mercuric chloride Disinfection Effect, but considers its serious environment pollution, and this laboratory is stopped to use this reagent and tested.
Plant is cultivated the general sucrose of using as carbon source, in order to reduce test tube flowers cost of manufacture, this experiment replaces sucrose with white granulated sugar, by the variable concentrations white granulated sugar kwan-yin seat lotus explant being expanded numerous multiple affects experimental result and shows, when white granulated sugar concentration is brought up to 40g/L, therefore expand numerous multiple and also be upgraded to the highlyest thereupon, also will continue to explore the concentration that whether increases white granulated sugar, can obtain the numerous multiple of higher expansion.Certainly white granulated sugar excessive concentration also can affect the osmotic pressure of medium, and is unfavorable to plant growth, therefore needs contrived experiment to explore kwan-yin seat lotus and expands the limit that numerous middle white granulated sugar concentration is used.
During the expansion of kwan-yin seat lotus was numerous, the 6-BA that adds variable concentrations in MS did not obtain the numerous seedling of desirable expansion, but obtains more callus, and this point is just the opposite in the black suede Alocasia amazonica Bud induction experimental result of research with Huang Qingfeng.The experimental result of Huang Qingfeng shows that BA concentration is in 4 ~ 10 mg/L scopes, black suede Alocasia amazonica all can induce Multiple Buds and breed, and the raising along with 6-BA concentration in the medium, inducing clumping bud rate and growth rate also improve (Huang Qingfeng thereupon, black suede Alocasia amazonica terminal bud is cultivated and Fast-propagation, Fujian hotwork science and technology, 2002,27 (3): 9-10).But black suede Alocasia amazonica belongs to aroid, and kwan-yin seat lotus is crassulaceae plants, different plants have very large difference to the requirement of hormone, although archusia and mitogen add simultaneously, common coordinate plant growth, though the physiological action of all kinds of plant hormones has relative specificity, but the various physiological effects of plant are general performances of variety classes hormone interaction.
Kwan-yin seat lotus explant adds NAA and obtains and expand preferably numerous effect in MS.But NAA concentration can not be too low, when only adding NAA0.3mg/L, the numerous multiple of expansion of kwan-yin seat lotus also is lower than control group (not adding NAA), and NAA concentration rises to 0.6 mg/L, expands numerous multiple and reaches the interior maximum 3.81 of this experimental design scope, along with NAA concentration continues to be increased to 0.9 mg/L, expand numerous multiple and be decreased significantly, experimental result obviously shows, when white granulated sugar concentration is 40g/L, kwan-yin seat lotus is expanded numerous multiple when NAA0.6 mg/L, and it is best to expand numerous effect.This experiment is difficult to be understood that adding a small amount of NAA does not promote that not only the explant expansion is numerous, and it is numerous to suppress on the contrary its expansion, and this may be relevant with kwan-yin seat lotus Endogenous Hormone Contents in Vitro.
Because kwan-yin seat lotus growth fraction is slower, it can be survived more than 4 months in little blake bottle, if culture vessel increases, medium content increases, and will prolong viewing and admiring the time of kwan-yin seat lotus.The test tube flowers manufacture craft of kwan-yin seat lotus is simple, the space that takies is little, the time of viewing and admiring is long, and the test tube flowers all grow under aseptic condition, need not to water, the management such as fertilising, can not bring the pollutions such as earth insect to environment yet, can beautify indoor environment, bring again the enjoyment of natural beauty to people, therefore have the very fine prospect of marketing.
Claims (6)
1. the preparation method of an angiopteris esculenta tube flowers, it is characterized in that: take the tubercle of kwan-yin seat lotus as explant, in expanding breeding culture medium, induce and produce budlet, budlet is connected in the vial that colored medium is housed cultivates the angiopteris esculenta tube flowers that acquisition can Gong view and admire; Described expansion breeding culture medium is: MS+0.6 mg/L NAA, agar 8 g/L, sugared 40 g/L, pH value are 5.8-6.2.
2. the preparation method of angiopteris esculenta tube flowers according to claim 1, it is characterized in that: described explant obtains as follows: kwan-yin seat lotus is cut off from the bottom, remove stem blade on every side, make it expose one section stem, keep terminal bud 6-8 blade on every side, walk stem and also do similar processing; With flowing water flushing, wash dust off and be clipped in impurity in the blade, with distillation washing 2-3 time, use again 70% alcohol-pickled 2 minutes, constantly shake; Outwell alcohol after two minutes, with 30% 84 thimerosals sterilization 30 minutes, also constantly shake during sterilizing with 84 thimerosals; Outwell 84 thimerosals after 30 minutes, use sterile water wash 3-4 time; The stem section of turning white in the budlet bottom of cleaning is cut, and sagging leaf is cut.
3. the preparation method of angiopteris esculenta tube flowers according to claim 1, it is characterized in that: the sugar in the described expansion breeding culture medium is white granulated sugar.
4. the preparation method of each described angiopteris esculenta tube flowers according to claim 1-3 is characterized in that: induce the condition of cultivation to be in expanding breeding culture medium: temperature is 22 ± 1 ℃, illumination every day 10 hours, and 40 days subcultures are once.
5. the preparation method of angiopteris esculenta tube flowers according to claim 1, it is characterized in that: described colored medium is: MS+ agar 8 g/L, sugared 40 g/L, pigment 0.2-25g/L, pH value are 5.8-6.2.
6. the preparation method of angiopteris esculenta tube flowers according to claim 5, it is characterized in that: described pigment is lemon yellow, greyish purple, apple green or carmine.
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| CN105900844B (en) * | 2016-05-13 | 2018-07-31 | 大连民族大学 | A kind of miniature ornamental plant and its cultural method |
| CN112293253B (en) * | 2020-10-28 | 2022-02-18 | 中国科学院昆明植物研究所 | Isolated preservation culture medium and isolated preservation method for Adiantum furiosaeanum |
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| 张良波等."屋顶长生花的离体培养和植株再生".《植物生理学通讯》.2005,第41卷(第1期),第56页. |
| 黄浅等."试管花卉的生产与应用".《亚热带农业研究》.2007,第3卷(第4期),第300-304页. |
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